ライフサイエンスコーパス: competition assay

1 acterial quorum sensing, is detected using a competition assay.

2 MRT, and STAMP) using our recently developed competition assay.

3 VS in human macrophage-like THP-1 cells in a competition assay.

4 hya site bases shown to be important in the competition assay.

5 The top hits were assayed in an in vitro competition assay.

6 devised a high-throughput fluorescence-based competition assay.

7 s, we have developed a functional antagonist competition assay.

8 binding enriched BMV replicase in a template competition assay.

9 o inhibited AA activation of PLC delta1 in a competition assay.

10 the values obtained by a fluorescence-based competition assay.

11 te 4-methylumbelliferyl sulfate (4-MUS) in a competition assay.

12 alian or avian viruses using a plasmid-based competition assay.

13 Center-of-Tree (COT) protein through fitness competition assays.

14 s were identified using ex vivo head-to-head competition assays.

15 neutralizing antibody were tested in direct competition assays.

16 and lysozyme than the wild type in in vitro competition assays.

17 ic acids were able to displace the tracer in competition assays.

18 ith Als2cr4 recombinant proteins and peptide competition assays.

19 ges at 37 degrees C measured by fluorescence competition assays.

20 n blotting, solid-phase epitope mapping, and competition assays.

21 based on coimmunoprecipitation and in vitro competition assays.

22 sing escape mutants, structure analyses, and competition assays.

23 ntially diminished viral fitness in in vitro competition assays.

24 ent to direct IAB5 to an ectopic promoter in competition assays.

25 has been difficult to define by conventional competition assays.

26 by using methylation protection and binding/competition assays.

27 ogies are generally applicable to any growth competition assays.

28 s confirmed by cell staining, knockdown, and competition assays.

29 pitopes were identified by ELISA and binding competition assays.

30 periments with deletion mutants, and peptide competition assays.

31 nd fitness during infection were measured by competition assays.

32 rom RAW264.7 cells by performing a series of competition assays.

33 F resistance mutations was studied in growth competition assays.

34 ion inhibitor as measured by exchange factor competition assays.

35 ability to grow in the lung, as measured by competition assays.

36 aller plaques and impaired fitness in direct competition assays.

37 M(-1)s(-1) at pH 7.4 through two independent competition assays.

38 nzyme-linked immunosorbent assay (ELISA) and competition assays.

39 ) coexpressing HER2 and EGFR was assessed in competition assays.

40 tested against wild-type pfcrt in co-culture competition assays.

41 ng, we used mutagenesis coupled with acetate competition assays.

42 was true for infectious virus, including in competition assays.

43 ld decreased GAS recovery in isogenic strain competition assays.

44 Using an in vitro RNA-binding competition assay, a unique cell-free assembly assay, an

45 d and changes in fitness were assessed using competition assays against genetically marked, surrogate

46 When the prsW mutant was tested in competition assays against its isogenic parent in the ha

47 irmed each of these phenotypes in one-on-one competition assays against otherwise-wild-type lacZ muta

48 In peptide competition assays, all HR-B mutants at residue 462 reve

49 lasmon resonance based vitronectin-PAI-1-SMB competition assay allowed us to conclude that 1) only th

50 yses,trans-protection analyses, and in vitro competition assays, allowed us to identify novel effecto

51 Intestinal persistence competition assays also demonstrated that the rpoN mutan

52 Competition assays among FBABIRT and BIRT derivatives de

53 mutagenesis, as shown by both a fluorescent competition assay and a polarity sensitive dye, badan.

54 Completion of the competition assay and complementation rescue system take

55 n binding assay, gel electrophoresis binding competition assay and confocal microscopy.

56 is dysfunctional in an Escherichia coli CRP competition assay and has been proposed as a potential s

57 Herein, the development of a TLR8 antagonist competition assay and its application for hit generation

58 n by the determination of IC(50) values in a competition assay and surface dissociation constants (K(

59 ceptor were measured using a (3)H-Mibolerone competition assay and varied from 18% of nilutamide bind

60 esponsive mRNA target candidates in both RIP competition assays and expression profiling experiments

61 Competition assays and molecular docking simulations sug

62 Guanosine triphosphate analog competition assays and mutagenesis analysis, performed t

63 Cross-competition assays and mutational analyses showed eviden

64 Using peptide competition assays and NMR spectroscopy, we conclude tha

65 Competition assays and structural mapping revealed two n

66 ads to reduced plaquing, fitness defect in a competition assay, and reduced tissue cyst formation in

67 determined using a fluorescence polarization competition assay, and the GFP expression in engineered

68 PP-stabilized microtubules was assessed by a competition assay, and their influence on microtubule po

69 inity of amychelin was determined using EDTA competition assays, and a biosynthetic cluster was ident

70 adhesin-deficient H pylori strains, chemical competition assays, and epithelial glycosylation affecte

71 m kinetic analyses, microtubule (MT) binding competition assays, and hydrogen/deuterium-exchange stud

72 In vitro gel shift assays, competition assays, and immunoprecipitations (IPs) revea

73 plex, the relative loop stability known from competition assays, and the relative loop size known fro

74 g, fitness was evaluated by growth curve and competition assays, and vanA presence was determined by

75 etection limits for unmodified toxins in the competition assay are comparable to values reported prev

76 ding affinity with lectins by a fluorescence competition assay are determined.

77 as characterized by kinetic, saturation, and competition assays at membrane preparations of Chinese h

78 ngth of the polyphenols was also tested in a competition assay based on the fluorescence quenching of

79 A competition assay, based on a validated chemical kinetic

80 Here we show, by using a direct competition assay between doxycycline-resistant and doxy

81 A competition assay between GDP and GDP analogues in the G

82 Additionally, data from a competition assay between SLA and single-stranded RNA (s

83 A competition assay between the DeltatoxRS and wild-type V

84 Results from an in vitro binding competition assay between the wild-type FlhD(4)C(2)-prot

85 We now show, using competition assays between ATP and GTP, that GTP is the

86 Furthermore, transfer rates and competition assays between ICESh95 and ICESh392, an SXT-

87 Analysis of competition assays between PA1 and TIF2 with an exogenou

88 We demonstrated that in in vivo competition assays between the rpoE mutant and the WT ma

89 In in vivo competition assays between the rpoN mutant and a wild-ty

90 Site-directed mutagenesis, competition assays between wild-type and mutant NDK prot

91 r dominance has repeatedly been supported in competition assays between Xenopus laevis and X. boreali

92 In a competition assay, both soluble CD163 and Fn14/TweakR we

93 within its central hydrophobic patch, and in competition assays, both CSP-1 and CSP-2 interacted with

94 Finally, we show how a competition assay can be set up to perform focused libra

95 We show that this general ELISA-based competition assay can be used to quantify small molecule

96 directed mutagenesis and sucrose octasulfate competition assays collectively indicate that the negati

97 An in vivo competition assay compared the colonization levels of th

98 ase in the competitive index in colonization-competition assays comparing the mutant strain with both

99 Peptide competition assays confirm that a candidate clathrin box

100 Competition assays confirmed that RSV MA binds inositol

101 Neutralization competition assays confirmed that the peptide NWFDITNWLW

102 Competition assays, cross-linking experiments, limited p

103 IC(50) (10.9 nM) derived from a biochemical competition assay (DELFIA) that measures the level of in

104 Efflux and substrate competition assays demonstrate that NAD is a preferred s

105 In vivo competition assays demonstrate that noninducing flavonoi

106 A tissue culture-based competition assay demonstrated that the HBD effectively

107 An HIF-1 binding and competition assay demonstrated that the HRE sequence fro

108 Competition assays demonstrated a marked advantage to be

109 Competition assays demonstrated that accumulation and lo

110 Competition assays demonstrated that Ad/GFP-ABP-PEG-HCBP

111 Competition assays demonstrated that both immunophilins

112 the direct assay is superior to that of the competition assay, detection limits for unmodified toxin

113 7)-10(8) M(-1)s(-1) range as determined by a competition assay developed here.

114 Data from molecular docking and substrate competition assays established that the primary molecula

115 Using a reporter-based high-throughput competition assay, followed by deep sequencing, we measu

116 mycin derivatives, which matched well with a competition assay for Hsp90alpha.

117 ng to target sites on immobilized DNA, and a competition assay for inhibition of the HMGA2-DNA comple

118 describe a novel, patient serum-independent competition assay for pathogenicity screening of FH19-20

119 We describe a supramolecular surface competition assay for quantifying glutamine uptake from

120 In addition, we developed a competition assay for the oligonucleotides between the s

121 As a demonstration of the potential of the competition assay for unlabeled toxin detection, we conc

122 tant analysis, a high-throughput subtractive competition assay, for genotypically defined M. tubercul

123 Solid-phase binding competition assays, glycoprotein blotting experiments, a

124 A fluorescence polarization competition assay has been developed to screen for inhib

125 Growth competition assays have been developed to quantify the r

126 Pooled mutant competition assays have shown that the Mycobacterium tub

127 Using an FA competition assay, host ACSLs were found to activate Ct

128 er analysis using lectin staining and lectin competition assays identified that AAV1 and AAV6 use eit

129 screening pluripotency-associated factors by competition assay; (ii) setting up an inducible compleme

130 Finally, we demonstrate an Affimer-based competition assay, illustrating the potential of Affimer

131 In an in vitro growth competition assay in mouse intestinal mucus, the rpoN mu

132 An SSA competition assay in which either diverged or identical

133 were similar to those obtained with a growth competition assay in which the relative proportion of ea

134 Oat1, mOat3, and mOat6, a fluorescence-based competition assay in Xenopus oocytes as well as wild-typ

135 their DeltasfaA and DeltasbnD mutants using competition assays in a murine abscess model and invasio

136 Competition assays in BALB/c mice revealed that mutants

137 By using antibody and oligonucleotide competition assays in electrophoretic mobility shift ana

138 s determined by yeast two-hybrid and in vivo competition assays in H. pylori cell-culture infection s

139 Consequently, competition assays in laboratory conditions may not refl

140 tro binding of biotinylated Cry proteins and competition assays in midgut protein vesicles showed wea

141 Finally, we show using competition assays in suckling mice that inhibition of m

142 -kappaB "isogenic" viral strains in pairwise competition assays in T-cell lines, primary cells, and t

143 ributes to pellicle formation and fitness in competition assays in the Gram-negative Pseudomonas aeru

144 During competition assays in vitro, an irs4-null (Deltairs4) mu

145 Using competition assays in vivo, we show that each of the hil

146 Competition assays indicate that although both substrate

147 Our competition assays indicate that dextran sulfate (8 kDa)

148 Substrate competition assays indicate that template misalignment,

149 Heparin competition assays indicated that this may be a result o

150 t most perform better than parental lines in competition assays, indicating that there is a trade-off

151 Previous bulk competition assays indirectly measured the loop lifetime

152 In a competition assay, inhibition of the megalin receptors r

153 age its incorporation, together with the RIP competition assay, into existing target prediction and v

154 The calorimetric competition assay introduced here overcomes this limitat

155 Pollen competition assays involving various combinations of AGL

156 The model-based competition assay is also unique in being able to order,

157 Using fluorescence anisotropy competition assays it is shown that PEA-15 competes for

158 With dialysis and competition assays, it was unambiguously demonstrated th

159 affinity was too weak to measure in the dye competition assays (Kd > 55 microM).

160 Using a new competition assay, NELF-A and NELF-B are each shown to a

161 In direct competition assays, no specificity for pregenomic RNA wa

162 ation required prior to analysis because the competition assay obviates the need to fluorescently lab

163 This flow cytometry-based growth competition assay offers advantages over current assays

164 We developed an ELISA-based competition assay on these air plasma-treated plates and

165 Head-to-head competition assays on agar plates indicate that two-dime

166 automated, and allows one to perform F-actin competition assays on similar sized proteins.

167 ptors using this method without performing a competition assay or increasing the protein concentratio

168 the paternal signal was not visible in sperm competition assays or as allelic imbalance in sperm.

169 d transurethrally either at an ~1 : 1 ratio (competition assay) or individually (monoinfection).

170 A significant advantage of the competition assay over reported profiling assays is the

171 Competition assays provide a means to assessing possible

172 Competition assays provide evidence that DNA strand sepa

173 Therefore, an equilibrium competition assay provides a quick and facile method to

174 e mixture of the two competing ligands, this competition assay provides accurate and precise values f

175 inant human MMP20 were compared by substrate competition assay, pull-down assay, and surface plasmon

176 ce resonance energy transfer and an in vitro competition assay revealed that activated GRs competed f

177 Moreover, an on-microarray competition assay revealed that the capture of the PPI b

178 Competition assays revealed a difference in the specific

179 In vitro growth competition assays revealed a fitness cost associated wi

180 Competition assays revealed excellent binding and select

181 In contrast to (Hs)CRM1, competition assays revealed that a human adapted mutant

182 Peptide competition assays revealed that the DEF2 peptide could

183 Competition assays revealed that the dominant HGM commen

184 , employing a fluorescence polarization (FP) competition assay, revealed that among several alpha/bet

185 Studies on sugar uptake, including competition assays, revealed that MRT was a sucrose and

186 By using a competition assay, several peptides derived from the BH3

187 Additional competition assays show that compounds were non 2-OG com

188 Substrate competition assays show that in solution zebularine is r

189 Coimmunoprecipitation and competition assays show that the trans-sialidase/parasit

190 In silico modeling and FRET-based competition assay showed that EGCG physically interacts

191 Direct in vivo competition assays showed a 5-fold competitive advantage

192 ELISA-based competition assays showed altered capacities of the aged

193 lysis of mutations in the hya site using DNA competition assays showed that apo-IscR most likely reco

194 Competition assays showed that both cleaved products are

195 Competition assays showed that PEDF can bind heparin and

196 Competition assays showed that the decrease in activity

197 Competition assays showed that the rate of binding (t((1

198 Competition assays showed that TIA-1 apparently binds wi

199 Competition assays showed that VP35 interacted specifica

200 CMP-Kdo synthetase inhibition and competition assays showing that Kdo and D-Ara, a precurs

201 es CLEC18A-1 binding to GLPS-F3, and a sugar competition assay shows that CLEC18 preferentially binds

202 ore allowed a highly robust and miniaturized competition assay sufficient for high-throughput screeni

203 Competition assays suggest that FH and C4BP have distinc

204 ut all phases of the cell cycle, and subunit competition assays suggested that hTERT-hTR interaction

205 ellency than single antagonists, and binding competition assays suggested that this enhanced repellen

206 Competition assays suggests that COBRA binds individual

207 We developed a mechanical single-molecule competition assay that allows online observation of bind

208 ed directly by the antibody array and (ii) a competition assay that employed unlabeled toxins as repo

209 comparably stable, as confirmed by a direct competition assay that established an equilibrium 55:45

210 a homogeneous fluorescence polarization (FP) competition assay that requires neither labeled primers/

211 We have developed a novel competition assay that uses a gold nanoparticle (Au NP)-

212 ence by flow cytometry allowed us to perform competition assays that demonstrated the high probabilit

213 Using a template competition assay, the core promoter of ca. 20 nucleotid

214 In a competition assay, the presence of purified rHagB decrea

215 rough the use of single-hit and multiple-hit competition assays, the catalytic core of pol eta was fo

216 oretic mobility gel shift assay (EMSA)-based competition assays, the kl-TSS was found to occupy a dif

217 egion 17-31 of PTH-(1-31) and assessing, via competition assays, their effects on binding to the wild

218 In dansylamide-competition assays, these designs showed 46-64-fold impr

219 (Con A) was determined using a fluorescence competition assay to be 0.289 +/- 0.003 muM, which repre

220 e (WT) virus were competed in a head-to-head competition assay to determine how different clones grew

221 We used a biosensor competition assay to determine whether there were corres

222 We have developed a novel, biosensor-based competition assay to directly address this important que

223 Here, we employ a competition assay to evaluate the role of this region in

224 essential genes and a high-throughput growth competition assay to measure fitness with unprecedented

225 We have developed a multiple-cycle growth competition assay to measure HIV-1 replication efficienc

226 Using a competition assay to measure relative affinities of puri

227 uently developed a photoaffinity probe-based competition assay to profile CDK inhibitors.

228 In this work, we used a competition assay to study how variations in the bound n

229 We used peptide competition assays to demonstrate that Cdh1p interacts d

230 c-specific fluorophore, Zinpyr-1, is used in competition assays to determine the kinetic and thermody

231 ase activity of I-AniI in vitro and utilized competition assays to probe the relationship between the

232 ite and complement these data with gel-based competition assays to provide a detailed structural unde

233 fluorescence complementation and two-hybrid competition assays to show that PTS represses KNOX1 prot

234 de-affinity profiling and nucleotide-binding competition assays, to characterize comprehensively (S)G

235 rescent probe binding has been assessed in a competition assay using the natural LFA-1 ligand ICAM-1

236 oinsulin (PPI), GAD65, and IA-2, followed by competition assays using a newly established "epitope-st

237 HB synthesis exist in agreement with in vivo competition assays using Acanthamoeba castellannii or hu

238 tant (Ki) of 0.02 nM measured in radioligand competition assays using cloned human receptors.

239 We then performed competition assays using clones of both morphs from diff

240 Moreover, in vitro competition assays using Cr-DFO and FO clearly demonstra

241 Moreover, competition assays using HS or removal of HS from the ho

242 as characterized by kinetic, saturation, and competition assays using membranes of Chinese hamster ov

243 Competition assays using nitrocefin efflux and covalent

244 In direct competition assays using pooled human urine inoculated w

245 Competition assays using purified molecules demonstrated

246 Competition assays using recombinant human NK(2) recepto

247 Moreover, an alternative competition assay utilizing Trp --> DNQX quenching for d

248 A competition assay was designed using TREX1 dominant muta

249 A fluorescence anisotropy-based coactivator competition assay was developed to measure the specific

250 efine the receptor binding domain of LsbB, a competition assay was performed in which a systematic co

251 Using DNA binding competition assays we found that Dna2 has substrate stru

252 tomic force microscopy, confocal images, and competition assays) we characterized this reagent and de

253 Using a competition assay, we demonstrated that a pyelonephritis

254 Using a plasmid competition assay, we have measured the stability of ori

255 Here, using a sensitive pairwise replication competition assay, we show that Vpr endows HIV-1 with a

256 NA affinity chromatography and cross-linking competition assays, we also demonstrate that the heterog

257 Using in vitro liposome binding and competition assays, we demonstrate that mVps24p selectiv

258 Using competition assays, we demonstrate that overexpression o

259 Using in vitro pulldown and competition assays, we demonstrate that this motif binds

260 Using gel-retardation and RNA/DNA competition assays, we found that RPA binds RNA with an

261 c cleft in the binding pocket, and using SPR competition assays, we observed that heme does not direc

262 Furthermore, a series of ligand competition assays were carried out on a single lectin s

263 ted by subtype C HIV-1 were constructed, and competition assays were carried out to compare the fitne

264 Flow cytometry-based receptor competition assays were developed to monitor concentrati

265 Saturation binding and competition assays were performed to determine equilibri

266 In this report, in vivo and in vitro competition assays were used to test whether females hav

267 The technique consists of a competition assay where the toxins in solution compete w

268 We test this prediction using an optogenetic competition assay whereby two targeted light beams indep

269 We also performed competition assays, which suggested that CaM and S100A1

270 monoclonal antibodies were used to develop a competition assay with a six-channel portable SPR biosen

271 face plasmon resonance-enhanced fluorescence competition assay with a surface-bound antibody is descr

272 involved in binding to cells, we performed a competition assay with C3 and RGD peptide or with a mono

273 tty acid to the antibody, and to implement a competition assay with free fatty acid (undecanoic acid)

274 e-binding site in tubulin, as confirmed by a competition assay with N,N'-ethylenebis(iodoacetamide) a

275 In a direct competition assay with palmitate, all the polyunsaturate

276 We also undertook a novel transfection competition assay with ZnT cDNAs to confirm that the dri

277 Using ligand competition assays with (125)I-labeled GH-RH antagonist

278 e found on MX-1 tumor membranes using ligand competition assays with (125)I-labeled GHRH antagonist J

279 Using competition assays with a selective MMP-12 inhibitor as

280 Binding competition assays with anti-CXCR4 antibodies show that

281 Competition assays with ApoE4, ApoE3, and Tau revealed t

282 Competition assays with CD4 and mAbs F105 and b12 sugges

283 ce of domain II also provides selectivity in competition assays with genomes from related phages.

284 Competition assays with hTSP-1 and Vn revealed the R1ab-

285 Competition assays with human kinesin-5 (Eg5) only parti

286 its were validated by RT-qPCR and in CRISPRi competition assays with individual GFP-expressing sgRNA

287 ll as in hypernucleation and paclitaxel site competition assays with isolated tubulin than the other

288 emolysis of chicken red blood cells (cRBCs), competition assays with monoclonal antibody (MAb) C179,

289 Competition assays with nucleotide analogs showed a rema

290 Competition assays with peptide-epoxide scanning librari

291 hat it confers a fitness advantage in growth competition assays with Pseudomonas putida.

292 PtdE-binding activities was confirmed using competition assays with PtdE-containing liposomes.

293 Through competition assays with sarcoplasmic reticulum vesicles

294 To investigate FVIII endocytosis, competition assays with soluble receptor ligands, bindin

295 Competition assays with the heterologous E. coli system

296 sRICs to bind HER2 or HER3 was determined in competition assays with unlabeled Fab or HRG on cells ex

297 he presence of deoxycholate and bile, and in competition assays with wild-type cells both in vitro an

298 However, in competition assays, with 6 of the 7 pairs, clade B strai

299 The utility of this competition assay would be greatly increased if the posi

300 Extending from the direct assay, the competition assay yields information on the presence, id