ライフサイエンスコーパス: conjugation

1 eversibility and Tub-tag for versatile probe conjugation).

2 d periodic frameworks with high in-plane pai-conjugation.

3 t of alternating alkene and phenyl groups in conjugation.

4 umab, used for antibody engineering and drug conjugation.

5 e uptake to immune evasion and subversion to conjugation.

6 through addition reactions that disrupt dye conjugation.

7 PDE6 as a retina-specific substrate of FAT10 conjugation.

8 has a crucial role in the regulation of IncC conjugation.

9 rial transglutaminase-mediated site-specific conjugation.

10 ge transfer and intramolecular through-space conjugation.

11 d represents a powerful strategy for polymer conjugation.

12 ar orbital energy levels and enhance the pai-conjugation.

13 ype IV secretion systems (T4SSs) can mediate conjugation.

14 8 as the E3 ligase that catalyzes SUMO2-PCNA conjugation.

15 ctive and irreversible scaffold for cysteine conjugation.

16 ity and safety, based on streptavidin-biotin conjugation.

17 m of the formation of MAC chromosomes during conjugation.

18 and ease of transfer to diverse bacteria by conjugation.

19 , possessing a heteroallylic B...Ge...Si pai-conjugation.

20 n the BPA metabolism, especially the sulfate conjugation.

21 cle was nonaromatic in nature due to lack of conjugation.

22 lized to the microcarriers surface for BMP-2 conjugation.

23 n a plasmid when it acquires another through conjugation.

24 disordered library peptide before and after conjugation.

25 with a maleimide terminal group for protein conjugation.

26 DC) and eight not previously associated with conjugation.

27 determine the set of genes required for IncC conjugation.

28 Rather than facilitating lipoate conjugation, ABHD11 associates with the OGDHc and mainta

29 ai* <- pai transitions arising from extended conjugation across MACR-oxide with overlapping contribut

30 SUMO conjugation activity alone was sufficient to target the

31 However, how this SUMO conjugation activity concentrates the proteins involved

32 enry reactions, as well as alkynylations and conjugation additions.

33 combined peptide stapling and PEG-fatty acid conjugation affords potent PYY analogs with >14 h rat ha

34 These linkers possess extended conjugation along one molecular axis, engendering low op

35 The 2D lattice enables full pai conjugation along x and y directions as well as pai cond

36 Neurotensin conjugation also improved the potency of morpholino ASO

37 e dipeptide linkers via heterogeneous lysine conjugation and bacterial transglutaminase-mediated site

38 anohoops allow studying the effect of cyclic conjugation and bending on the incorporated pai-systems.

39 branched carrier has multiple sites for drug conjugation and can reduce the need for multiple injecti

40 including surface functionalization, polymer conjugation and cross-linking, and biomolecular labeling

41 of horizontal gene transfer (HGT), including conjugation and cytoplasmic exchange through nanotubes.

42 erstanding of interactions between ubiquitin conjugation and deconjugation machineries and we examine

43 hed, and functionalized polymers for protein conjugation and drug delivery.

44 SpyCatcher003 enabled efficient VLP conjugation and extreme thermal resilience.

45 electronic and steric effect, including pai-conjugation and H-bonding.

46 Conjugation and hyperconjugation are closely related ste

47 d thermolysin, His-1084 was required for the conjugation and inhibition of acetylated thermolysin, wh

48 lkenals, which lead to MACs upon glutathione conjugation and metabolization.

49 macrocyclic compounds, consisting of diverse conjugation and metal chelation systems and imparting an

50 analytical methods to measure the extent of conjugation and modification of VLP based therapeutics.

51 ental interactions under the combined charge-conjugation and parity transformations, known as CP viol

52 into a helicene framework disrupts aromatic conjugation and provides a site for selective deprotonat

53 nt of glycopeptides, (4) solid-phase peptide conjugation and release of O-GalNAc glycopeptides using

54 This effect is largely independent of ISG15 conjugation and relies on ISG15 functional interaction w

55 demonstrates the critical role of continuous conjugation and strong electronic coupling between the G

56 as better associated with particle size than conjugation and was lowest for PPI-stabilized emulsions.

57 ned with oxygenation, cleavage combined with conjugation, and other uncategorized alterations.

58 is associated with improved selectivity, pai-conjugation, and reactivity at multiple points in the sy

59 lesterol metabolism and bile acid synthesis, conjugation, and transport, resulting in cholestasis, in

60 In the "extended conjugation" approach, the metals and entire ligands for

61 lizability, as numerous probes with multiple conjugations are needed to quantify a wide range of biom

62 challenging due to the heterogeneity of drug conjugation as well as the numerous post-translational m

63 We describe the development of a new "thiol conjugation assay" for identification of novel small mol

64 carried out, providing high yields of 2'-OH conjugation at predetermined sites.

65 lecule allows to rapidly confirm the Fc-drug conjugation at the chromatographic level.

66 not only through-bond but also through-space conjugation between imide groups, which leads to distinc

67 Here, we introduce conjugation between platinum(IV) prodrugs of cisplatin a

68 ty of the molecular analogues and continuous conjugation between the acid and the graphite surface.

69 corporating and combining a bridge for sigma-conjugation between the D and A units and a structure-di

70 extract, either unchanged or transformed by conjugation/binding.

71 chnically challenging, and in vivo bacterial conjugations (bioconjugations) have emerged as manufactu

72 T6SS and plasmid conjugation both involve cell-to-cell contact.

73 teracting protein RECQ5, promotes SUMO2-PCNA conjugation but inhibits SUMO1-PCNA formation.

74 ctively, these data demonstrate that protein conjugation by SUMO2 is critically involved in cognitive

75 te, the palladium(II)-aryl group facilitates conjugation by undergoing reaction with a second, cystei

76 e PEG-haloperidol conjugate, suggesting that conjugation can effectively prevent unwanted central eff

77 Avidin (mAv) with 28 sites for covalent drug conjugation can rapidly penetrate through the full thick

78 The ubiquitin (Ub) and Ub-like (Ubl) protein-conjugation cascade is initiated by E1 enzymes that cata

79 d copper-assisted azide-alkyne cycloaddition conjugation chemistries were employed to generate hetero

80 To gain a better understanding of the conjugation chemistry taking place at the interface of t

81 lectively functionalized using bioorthogonal conjugation chemistry.

82 This study suggests that Maillard conjugation could improve PPI emulsification properties.

83 Our results highlight the importance of gold conjugation decisions and imaging parameters to high-res

84 Predictive models were developed for conjugation efficiency and emulsification performance.

85 SpyCatcher003 conjugation enables nanodisplay of diverse symmetries to

86 o Ub's C terminus for Ub transfer reactions, conjugation enzymes often bind noncovalently and weakly

87 Ubiquitin conjugation enzymes promote ubiquitinylation and, thus,

88 these 2 properties are often amalgamated in conjugation enzymes.

89 this partially fused nanoring show that pai-conjugation extends around the whole macrocycle, and tha

90 The conjugation extent was negatively affected by the protei

91 ADC independent of antibody type and site of conjugation (Fab and Fc) in preclinical studies.

92 albumin, and other plasma proteins by lipid-conjugation facilitates ASO transport across endothelial

93 tokine release, suggesting that polypeptidic conjugation fails to affect the anti-inflammatory activi

94 l significance of the architecture of GalNAc conjugation for efficient uptake by target liver cells a

95 However, the costs and benefits of conjugation for plasmid and bacterial fitness are not we

96 rials with the emerging properties of radial conjugation found in cycloparaphenylenes (CPPs) and othe

97 An increase in conjugation further led to higher binding probability ow

98 n have been well studied(4-9), many roles of conjugation genes have been assigned based solely on seq

99 synthesizing the regulatory proteins of the conjugation genes, and constructed an in vivo system unc

100 We identified 27 conjugation genes, comprising 19 that were previously id

101 um sensing to determine when to activate the conjugation genes.

102 n, AcaDC activates the transcription of IncC conjugation genes.

103 Surprisingly, conjugation had little impact on overall frequencies, al

104 Although aspects of IncC plasmid conjugation have been well studied(4-9), many roles of c

105 Conjugation improved the physical stability (droplet siz

106 he resultant M/COF-DB exhibited extended pai-conjugation, improved crystallinity, enhanced stability,

107 polysaccharides (PPS) and modulation of the conjugation in order to obtain desired functional ingred

108 rovides substrate specificity for SUMO2-PCNA conjugation in response to TRC remains unknown.

109 Conjugation in the cytosol was more efficient when SpyCa

110 w that RappLS20 is sufficient for activating conjugation in vivo.

111 tance genes in bacterial populations through conjugation, in the absence of direct selection pressure

112 to an intermolecular path when the degree of conjugation increased.

113 While lipid conjugation increases tissue exposure in mice and reduce

114 mpetence and competence development inhibits conjugation, indicating that both processes are mutually

115 our previous results show that activation of conjugation inhibits competence and competence developme

116 lar weight multifunctionalized PEG-maleimide conjugation intermediate.

117 ing cross-conjugated, half cross-conjugated, conjugation-interrupted and linearly conjugated systems

118 Because Ub-conjugation is a reversible process, the aim of this stu

119 oparticles of these systems before and after conjugation is also confirmed by quantum mechanical mode

120 The utility of the TzB-mediated NCys conjugation is demonstrated by rapid and non-disruptive

121 The conjugation is efficient, does not compromise the affini

122 The calculations illustrate that this conjugation is interrupted in a GCC acetic acid system,

123 Optimal conjugation is of critical importance, as the Ab-AuNP bi

124 n anionic cyclopentadienyl unit, switches on conjugation, leads to a >200 nm red-shift in the absorba

125 bly minor role of the change in pai-electron conjugation length (and therefore conjugated backbone pl

126 The carbon-carbon double bond (C=C) conjugation length of carotenoids (N), generally between

127 which are nearly circular with high aromatic conjugation lengths for a given aromatic ring count.

128 of aromatic poly(carboxylic acid)s with high conjugation lengths.

129 and small non-coding RNAs requiring the LC3-conjugation machinery for packaging and secretion.

130 RNAs against all components of the ubiquitin-conjugation machinery using high-content microscopy.

131 urthermore, their secretion requires the LC3-conjugation machinery, neutral sphingomyelinase 2 (nSMas

132 Here we report a new chemical conjugation method which photocages highly reactive but

133 the fundamentals of biopolymers and chemical conjugation methods to introduce cross-linking groups ar

134 lable with traditional nucleophilicity-based conjugation methods.

135 ing issues associated with existing chemical conjugation methods.

136 ing ADCs are lacking as they are a different conjugation modality.

137 udy provides an understanding the underlying conjugation nature of both the Aptamer-SWNT and the Apta

138 Glucose conjugation nevertheless increases nuclear delivery ca 2

139 Quantitative conjugation occurs over the course of a few hours, at am

140 Conjugation of 2-cyanobenzothiazole (CBT) with N-termina

141 In Enterococcus faecalis, conjugation of a Cas9-targeted plasmid was enhanced by a

142 sults: We successfully synthesized a DML for conjugation of a fluorescent dye and (18)F.

143 The probe design involves conjugation of a seminaphthorhodafluor dye with a disele

144 This conjugation of a small nanoparticle with chiral recognit

145 s that allow site-specific and multiple-site conjugation of a wide assortment of molecules on both th

146 the key elements allowing for mastering the conjugation of Ab to AuNP and rationalizing, at the mole

147 first enzymes of translation, catalysing the conjugation of amino acids to cognate tRNAs for protein

148 Click chemistry enables conjugation of an azide molecule tagged with a fluoresce

149 Conjugation of antisense oligonucleotide (ASO) with a va

150 into two site-specific anchor points for the conjugation of any alkyne-containing payload of choice.

151 linositol-3 kinase complex I (PI3KC3-C1) and conjugation of ATG8/LC3 proteins to phagophore membranes

152 The conjugation of azido moiety to chitin backbone strongly

153 unctionalization, and this makes the uniform conjugation of bioactive ligands very challenging.

154 Conjugation of ciprofloxacin to lectin probes enabled bi

155 This work demonstrates that the conjugation of CpGODN to GBS67 in conjunction with adsor

156 These include conjugation of CpGODN with proteins or encapsulation/ads

157 he biosensors were constructed by sequential conjugation of CPNTs and aptamer molecules on the IMEs,

158 M(2)R-selective MR antagonists based on the conjugation of di- or tripeptides to M(2)R-preferring di

159 Ub-conjugation of human NHE3 by Nedd4-2 regulates endocytos

160 ulation, catalyzes ATP- and Mg(2+)-dependent conjugation of L-glutamate primarily to the 8-carboxyl o

161 in base (LCB) that is normally formed by the conjugation of l-serine and palmitoyl-CoA.

162 Conjugation of laminarin to ovalbumin reduced its IgE bi

163 This design approach, dubbed "conjugation of ligands and antibodies for membrane prote

164 We conclude that while conjugation of long-chain Vi generates T-dependent antig

165 So conjugation of mass spectrometry and scFv antibody provi

166 s with chemical tags for subsequent targeted conjugation of molecular cargos via efficient chemistrie

167 We report a novel conjugation of N-terminal cysteines (NCys) that proceeds

168 t (k(2) : ~5000 m(-1) s(-1) ) and reversible conjugation of NCys with 2-formylphenylboronic acid (FPB

169 short oligonucleotides and does not require conjugation of organic dyes with nucleic acids.

170 Conjugation of ovalbumin to laminarin increased its immu

171 The location/site of conjugation of payload-linker can have an effect on ADC

172 Conjugation of peptides or whole proteins to VLPs can be

173 Conjugation of pleuromutilin is an attractive strategy f

174 ed on the investigation of laccase-catalyzed conjugation of potato protein (PPT) with selected pectic

175 xhibited the highest specificity towards the conjugation of PPT/sugar beet pectin.

176 We hypothesize that the conjugation of quinidine to a polymer will permit its us

177 Conjugation of recombinant human deoxyribonuclease I (rh

178 and cost saving post-translational, covalent conjugation of recombinant proteins in plants.

179 Conjugation of shishijimicin A enediyne payloads through

180 associated with the production and chemical conjugation of signal-amplifying enzymes.

181 We demonstrate that the multiple-site conjugation of ssODN to Cas9 significantly increases the

182 Here we investigate the conjugation of tandem Hepatitis B core (tHBcAg) VLPs and

183 The simplest interface relied on direct conjugation of the antibody to the polymer membrane and

184 ed aromatic ring to enhance the rigidity and conjugation of the dye moieties.

185 -chair conformation due to the disruption of conjugation of the macrocycle because of the presence of

186 Conjugation of the moderate bone-binding drug risedronat

187 tic stability, dephosphorylation, and biotin conjugation of the peptides are indispensable for formin

188 The conjugation of the polyethylene glycol-coated Si nanopar

189 Inhibition of neddylation, the conjugation of the small ubiquitin-like protein NEDD8 to

190 =2.3 eV), unambiguously proving the superior conjugation of the vinylene-linked 2D CPs using the HWE

191 ide building blocks of medium-to-large size, conjugation of these subunits, and final assembly were a

192 Conjugation of this backbone-modified peptide to a camel

193 lso covered the design and chemistry for the conjugation of TLR2 agonists to antigens, carbohydrates,

194 e applied our protocol for the site-specific conjugation of two proteins with unique cysteine residue

195 Few chemical methods exist for the covalent conjugation of two proteins.

196 effectors, such as SdeA, that catalyzes the conjugation of Ub to a serine residue of target proteins

197 itin interaction surface is required for the conjugation of ubiquitin to K644.

198 PS are E3 ubiquitin ligases, responsible for conjugation of ubiquitin to specific cognate substrates.

199 Here, we demonstrated that with the conjugation of zwitterionic poly(carboxybetaine) (pCB) p

200 This included the amino acid conjugations of host bile acids that were used to produc

201 iyaura cross-coupling is useful for multiple conjugations of peptides in conjunction with conventiona

202 lyzed alkyne-azide [3 + 2] cycloaddition for conjugation onto pleuromutilin.

203 iption and translation of long virulence and conjugation operons in many Gram-negative bacterial path

204 hindering its potential to rival widely used conjugation pairs, such as streptavidin-biotin.

205 the therapeutic potential of modulating PAR conjugation (PARylation): PAR polymerase (PARP) inhibito

206 Hence, the LC3-conjugation pathway controls EV cargo loading and secret

207 of the SUMO (small ubiquitin-like modifier) conjugation pathway.

208 ymers that combine the linear intramolecular conjugation pathways commonly associated with organic el

209 stituents allow a welcoming extension of the conjugation pathways.

210 By leveraging the conjugation platform, we successfully engineer INS-1E, a

211 ish-peroxidase-labeled nanobody using a mild conjugation procedure as a detection antibody throughout

212 This new NCys conjugation proceeds via a thiazolidine boronate (TzB) i

213 Here, we evaluated the conjugation process by comparing trastuzumab and trastuz

214 om the PEG-maleimide polymers throughout the conjugation process.

215 uperfamily use thiol esters to form covalent conjugation products upon their proteolytic activation.

216 The main conjugation promoter, Pc, is by default repressed by a r

217 This improved understanding of IncC conjugation provides a basis for the development of new

218 Zwitterionic polymer conjugation provides the possibility for a favorable, no

219 M(PEG)2 linker but combinations of carriers, conjugation ratio of peptide to carrier, or choice of ad

220 We have developed a conjugation reaction based on the thia-Michael addition

221 ed to the understanding of laccase-catalyzed conjugation reaction for the controlled synthesis of con

222 alactan, were evaluated as substrates in the conjugation reaction-catalyzed by laccases (Trametes ver

223 n a structural transformation related to the conjugation reaction.

224 of peptides in conjunction with conventional conjugation reactions performed in sequence.

225 demonstrated that oxidation, reduction, and conjugation reactions were the most important routes of

226 has been developed for the synthesis of the conjugation-ready ligand as well as the connection of it

227 We here introduce a conjugation-ready TLR4 ligand, CRX-527, a potent powerfu

228 , we report the first total synthesis of the conjugation-ready trisaccharide repeating unit of Pseudo

229 stability compared to conventional cysteine conjugation reagents, increasing the reaction rate by >3

230 but these strategies use either nonspecific conjugation resulting in mixtures, or site-specific meth

231 ormation that inherently yields poor lateral conjugation, resulting in 2D materials that are weakly c

232 peptide sequence dependence of this amidine conjugation reveals a tripeptide tag CIS that allows fac

233 Suitable platforms and conjugation routes are urgently needed to convert the pr

234 imary sequence, and localization of the drug conjugation site of the ADC using high-resolution Fourie

235 The conjugation site of the linker-drug on the antibody was

236 roteolysis or deconjugation at the fusion or conjugation site present further issues.

237 actional solvent accessibility, pK(a) of the conjugation site, surrounding residue's pI, and electros

238 s, including their large size, array of drug conjugation sites, and heterogeneous compositions contai

239 nation of different SSTR2-targeting ligands, conjugation sites, and payloads led to the discovery of

240 ttributes including DAR, positional isomers, conjugation sites, occupancy, and microvariants, which p

241 entarity-determining regions and the payload conjugation sites.

242 apeutic efficacy because of the variation in conjugation stability, and thus they need to be well cha

243 on as well as the identification of multiple conjugation states.

244 ns, overcoming the limitation of traditional conjugation strategies that rely on the number of innate

245 biocatalyst enables the development of novel conjugation strategies to prepare antibody-TNM conjugate

246 e report an efficient MTM 2'-oxime (MTM(ox)) conjugation strategy for rapid MTM diversification.

247 al employ an elegant microRNA (miRNA or miR) conjugation strategy to unleash the potential of targete

248 ated to hundreds of proteins and targets its conjugation substrates for proteasomal degradation, but

249 Small ubiquitin-like modifier (SUMO1-3) conjugation (SUMOylation), a posttranslational modificat

250 tion is dependent on the function of the ATG conjugation system, which mediates LC3 lipidation.

251 Bacterial conjugation systems are members of the large type IV sec

252 ype IV secretion systems (T4SSs), termed the conjugation systems, transmit mobile genetic elements (M

253 Nevertheless, ADC conjugation techniques and processes may introduce intri

254 e theoretical calculations showed that hyper-conjugation, the main underlying factor hindering the ae

255 versible multimerization and versatile probe conjugation through a novel double tag (Strep-tag for re

256 With a surface area of 801 m(2) g(-1) , full conjugation throughout the carbon backbone, and an elect

257 linker (DML) that facilitates site-specific conjugation to a cysteine residue-bearing antibody fragm

258 f talazoparib (TLZ) was achieved by covalent conjugation to a PEG(40kDa) carrier via a beta-eliminati

259 e formation occurred, including LC3-membrane conjugation to autophagic precursors.

260 ly reactive lysine residue for site-specific conjugation to beta-lactam linker-functionalized siRNA.

261 r proof of concept, we demonstrate effective conjugation to biotin as a model for flexible co-targeti

262 ed from simple binding to direct cleavage by conjugation to bleomycin A5 in a manner that imparts RNA

263 2 from C. jejuni WP2-202 was transferred via conjugation to C. jejuni NCTC11168 Nal(+), transconconju

264 cGMP also enhanced within minutes ubiquitin conjugation to cell proteins.

265 by a conserved histidine residue and allows conjugation to cell surface glycans.

266 ross endothelial or epithelial monolayers is conjugation to cell-penetrating peptides (CPPs); however

267 nts the benefits of cell-penetrating peptide conjugation to enhance the potential of peptide aldehyde

268 ess carboxylic acid functionality for simple conjugation to flexible tethers.

269 nse against these electrophilic molecules is conjugation to glutathione, then metabolization into mer

270 amides have attracted attention due to prior conjugation to nonsteroidal anti-inflammatory drugs (NSA

271 n our laboratory demonstrated that ubiquitin conjugation to OAT1 leads to OAT1 internalization from t

272 hains could be used for peptide extension or conjugation to other molecules (e.g., by olefin metathes

273 TLR2 agonists such as lipidation of antigen, conjugation to polymers, phosphonic acids, and other lin

274 novel delivery system of CpGODN based on its conjugation to serve as anchor for liposomes.

275 Their conjugation to the chitosan chain saves their antibacter

276 assay, that drug activity is retained after conjugation to the polymer, supportive of retention of e

277 Conjugation to the small ubiquitin-like modifier (SUMO)

278 tudy reports a synthetic bacterium that uses conjugation to transfer toxic genes that selectively kil

279 SO(2)R) and a primary amino group at C-7 for conjugation via reductive amination.

280 sily customized (e.g., PEGylation and ligand conjugation) via various functional groups incorporated

281 eport an exhaustive survey of the methods of conjugation, via strategies of physisorption and chemiso

282 imultaneous (89)Zr-radiolabeling and protein conjugation was performed in one-pot reactions containin

283 To resolve drawbacks related to cysteine conjugation, we have invented a phage-display technique

284 The peptide conjugations were carried out in high yield (90%) with t

285 al communication including in the process of conjugation, which has large economical and health-relat

286 class of materials that combine extended pai-conjugation with a permanently microporous skeleton.

287 putative ion channel pore, for site-specific conjugation with a photoswitchable pore blocker.

288 CNC scaffold, which was further modified by conjugation with a protein - transferrin (Tf) for target

289 oxytocin derivatives were engineered through conjugation with fatty acid moieties that are known to e

290 d in a PDMS microfluidic channel, is used in conjugation with immuno-magnetic separation of cancer ce

291 characteristics like on-target delivery, and conjugation with immunotherapeutic reagents enhances imm

292 in LC3/GABARAP lipidation, as one target of conjugation with multiple copies of LC3/GABARAP.

293 ion efficiency (i.e., CR energy transfer) by conjugation with multiple CR-absorbing (water-soluble) a

294 d distribution and are adaptable to covalent conjugation with multiple substrates.

295 roblems with their use have been overcome by conjugation with N-acetylgalactosamine, an adduct that t

296 They readily undergo conjugation with reducing sugars at the desired 1:1 stoi

297 , an underutilized natural polysaccharide in conjugation with soy protein isolate (SPI) as an encapsu

298 After conjugation with three Alexa Fluor 680 (AF680) fluoropho

299 tensively in bioelectronic devices, often in conjugation with viologen moieties which act as electron

300 Bleb formation is independent of ubiquitin conjugation within the bleb, but strictly dependent on P