ライフサイエンスコーパス: conserved residue

1 tion on lysine 403 (K403), an evolutionarily conserved residue.

2 oskeletal GTPase Septin7, at an evolutionary conserved residue.

3 fibronectin (FN1) variants affecting highly conserved residues.

4 bridges, or hydrophobic interactions between conserved residues.

5 ds the top face or to be at buried or highly conserved residues.

6 She2p binding pocket are composed of highly conserved residues.

7 ubules by using small groups of evolutionary conserved residues.

8 two families displaying MFSD2A mutations in conserved residues.

9 ally interacts with COQ7 through a series of conserved residues.

10 ysis reveals that prolines constitute 17% of conserved residues.

11 to a wide range of mutations, even in highly conserved residues.

12 to the anticodon domain, and disrupt highly conserved residues.

13 da motor and to investigate the roles of the conserved residues.

14 ion in xanthophores, phosphorylates Plin6 on conserved residues.

15 trinuclear zinc center, coordinated by nine conserved residues.

16 tinguish regulatory sites from nonfunctional conserved residues.

17 8A and K505A R508A, alanine substitutions at conserved residues abolished GP processing and membrane

18 Mutation of these conserved residues abolishes GluN2C binding and has no f

19 The variants disrupt highly conserved residues across the protein.

20 of hydrogen bond interactions between highly conserved residues across the RNase dimer interface that

21 and bi-allelic missense variants localize to conserved residues; all but one such variant occur withi

22 es seems to be mediated by cation binding to conserved residues along the three-fold axis.

23 The missense mutation concerns a highly conserved residue among species, located in the region i

24 ine functional roles for each of these eight conserved residues and allow us to propose a sequential,

25 : NM_001273.3), affect evolutionarily highly conserved residues and are predicted to be deleterious.

26 ed missense mutations in PADI3 affect highly conserved residues and are predicted to be pathogenic; p

27 in binding produce different arrangements of conserved residues and customized interfaces on the hydr

28 ar head of H3 HA that is comprised of highly conserved residues and is distinct from the receptor bin

29 ent of the H-bonding network associated with conserved residues and structural water molecules.

30 up the recombinant protein when four highly conserved residues and the 70-amino acid small alpha+bet

31 ifest as superfamily and functional-subgroup conserved residues, and as pairwise correlations.

32 amino acid residues of the active quintet of conserved residues, and of surface-exposed residues for

33 Both mutations affected conserved residues, and R291Q is orthologous to R294, wh

34 Tyr676Cys]) result in substitution of highly conserved residues, and six of seven cluster within EC d

35 We hypothesize that deleterious variants at conserved residues are enriched in severely affected pat

36 Mutational analysis demonstrates that two conserved residues are essential for Pit2 function.

37 d that DinB variants with mutations in these conserved residues are less effective at stabilizing Rec

38 These conserved residues are likely to perform the same critic

39 Highly conserved residues are located in the extensive interfac

40 A highly conserved residue Arg(97) in the CDR3alpha loop played a

41 rent de novo mutations, affecting the highly conserved residue Arg138 of P5CS, cause an autosomal-dom

42 amino acid substitutions of the same highly conserved residue, Arg138 in P5CS.

43 Mutations of conserved residues around this pocket affect activity of

44 However, the non-conserved residues Asn-120, Arg-357, and Asn-373 form di

45 Site-directed mutagenesis of these highly conserved residues (Asp-192, Arg-258, Phe-272, Glu-295,

46 rvation of another LBHB (2.47 A) between two conserved residues, Asp233 and Asp246, suggests that LBH

47 structures suggest the involvement of highly conserved residues at the active site.

48 nine substitutions introduced to four highly conserved residues at the C terminus and one at the N te

49 that cdG binds to FliI in a pocket of highly conserved residues at the interface between two FliI sub

50 Of interest, the evolutionarily conserved residues at the luminal interface of the third

51 and found that they contained nearly all the conserved residues characteristic of SPaseII family memb

52 to identify signatures involving the role of conserved residues, conserved contacts, and downstream s

53 ovel molecular switch involving the strictly conserved residue D74(2.50).

54 This tight coordination by a network of conserved residues defines a sequential, around-the-ring

55 ore sequences, we were able to highlight key conserved residues driving essential elements of TCR rec

56 revealed dramatic changes to the exposure of conserved residues during virus maturation.

57 The five missense mutations affect highly conserved residues either in the sixth repeat of the RCC

58 Previous studies identified five conserved residues (ELEFN(50-54)) in the N-terminal doma

59 ialized active site consisting of two highly conserved residues equivalent to SOD5 Glu-110 and Asp-11

60 of these mutations were localized near or at conserved residues essential for diiron ion coordination

61 While this mechanistic step is driven by conserved residues, evolutionarily variable residues are

62 a conformational epitope composed of highly conserved residues, explaining its broad neutralization

63 st, most missense mutations affecting highly conserved residues failed to eliminate ACC2 function.

64 heet within an alpha+beta-fold to coordinate conserved residues for catalysis.

65 xed to the KDEL receptor binds cargo via its conserved residues for transport to the ER.

66 ar beta-helical (solenoid) conformation with conserved residues forming the tightly packed core and p

67 Conserved residues from a diverse set of BAM2 orthologs

68 ried in the cytosolic domain and chelated by conserved residues from CTD and the His-Cys-His (HCH) mo

69 Conserved residues from the signal peptide and residues

70 croscopic analyses to demonstrate that these conserved residues function after assembling HIV-1 Gag h

71 Unexpectedly, when substituting the conserved residues Gln(758) (Q motif) or Lys(785) (I mot

72 onstriction point consisting of three highly conserved residues - Gln(232/585)-Asp(262)/Asn(623)-Tyr(

73 rg62 forms a salt bridge with another highly conserved residue, Glu38.

74 The highly conserved residue H75 is located at the protomer interfa

75 Alanine substitution of the conserved residue H98 in prM disrupts the switch by inhi

76 individual BTHS mutations at evolutionarily conserved residues has identified seven distinct loss-of

77 A lipid binding groove and clusters of conserved residues highlight potential functional sites.

78 f Vibrio cholerae WT ApbE and mutants of the conserved residue His-257, to understand its role in sub

79 sfer coupled with a proton transfer from the conserved residue, His548.

80 on other NAOX-nucleic acid complexes reveals conserved residues important for recognition and demethy

81 The de novo variants affect a single conserved residue in a zinc finger motif crucial for DNA

82 by the orientation of arginine 66, a highly conserved residue in Anthozoan PCFPs.

83 se mutation c.591C>A p.Glu197Asp in a highly conserved residue in exon 4 of ACTA1.

84 In this study, we identified a conserved residue in Lpro that is involved in its intera

85 uenced by Polo-mediated phosphorylation of a conserved residue in Miro, which positively regulates Mi

86 We speculate a conserved residue in S5 (S412 in Shaker), within van der

87 Surprisingly, mutation of the conserved residue in Set2 (R195C) similarly resulted in

88 ral studies, we hypothesized that Asn-169, a conserved residue in the AAG active-site pocket, contrib

89 mical mechanism: direct phosphorylation of a conserved residue in the activation loop (Cdr2-T166 and

90 l analyses reveal that a single, differently conserved residue in the cap domain of either AUM or chr

91 .Thr270Ala missense variant affects a highly conserved residue in the DBL homology domain, which is r

92 C479 is a highly conserved residue in the extracellular domain of ENaC an

93 utagenic screen we identified K165, a highly conserved residue in the extracellular vestibule of the

94 I109 is a highly conserved residue in the forkhead box (Fox) domain of FO

95 T835M alters a conserved residue in the hinge region of UNC5C, and in v

96 ors, one of which (Arg303Cys) is at a highly conserved residue in the N/D loop.

97 Finally, we show that an evolutionarily conserved residue in the PLL domain is critical for olig

98 A missense mutation in a highly conserved residue in the RacGEF domain results in normal

99 Further, our results show that changes to a conserved residue in the RdRp from different species gro

100 This A178D missense mutation, affecting a conserved residue in the second immunoglobulin-like doma

101 both in vivo and in vitro on an evolutionary conserved residue in the switch II domain.

102 phosphorylates a subset of Rab GTPases on a conserved residue in their switch-II domains (PMID: 2682

103 erone-client interactions by changing highly conserved residues in a putative client-binding groove.

104 y more new damaging and missense variants at conserved residues in cases than in controls (P = 1.6 x

105 propose that the amino acid patch of highly conserved residues in DinB-like proteins provides a mech

106 Interestingly, there are six differentially conserved residues in DPs affording either the (+)- or (

107 Disease-associated point mutations of conserved residues in either the Rab3GAP1 (T18P and E24V

108 By targeting conserved residues in Escherichia coli RNAP, we are able

109 BL patients, are either truncating or affect conserved residues in functional domains, thus supportin

110 functional differences between positionally conserved residues in how they influence recombinase-tar

111 We show that ERK phosphorylates Dicer on two conserved residues in its RNase IIIb and double-stranded

112 With conserved residues in mammalian Hig1 proteins and Cox3/C

113 ys, we performed an in vivo analysis of well-conserved residues in Msh3 that are hypothesized to be r

114 d a comprehensive mutational analysis of the conserved residues in ORF18, which is a highly interconn

115 Alteration of highly conserved residues in PhoU by site-directed mutagenesis

116 erform structure-function analysis of highly conserved residues in Porcn and Wnt3a.

117 Many cohesinopathy mutations target conserved residues in Scc2 and diminish Ct Scc2 binding

118 y)-both affecting functionally important and conserved residues in SEC61.

119 unctional significance of two evolutionarily conserved residues in SETD2 that are recurrently mutated

120 We show that conserved residues in site alpha influence enzyme proces

121 s O-linked mannose (O-Man) glycans at highly conserved residues in specific extracellular cadherin do

122 Mutagenesis of conserved residues in TglB identified several key residu

123 showing its architecture and the position of conserved residues in the active site.

124 By mutating conserved residues in the beta-barrel domain of this pro

125 Conserved residues in the C-terminal segment interact wi

126 erpesviruses revealed the presence of highly conserved residues in the central portion of the UL37 pr

127 We report that mutation of three strongly conserved residues in the ectodomain has no effect on ta

128 The variants were located at highly conserved residues in the FHF2A inactivation particle th

129 We focused on two highly conserved residues in the HA stem region: HA2 position 5

130 Previous studies have shown that highly conserved residues in the inner domain of gp120 are requ

131 NCT variants that harbor mutations at highly conserved residues in the lid region inNCT-deficient cel

132 By revealing precisely when conserved residues in the major homology region are requ

133 Highly conserved residues in the major homology region are requ

134 Herein, we targeted conserved residues in the MeaB switch I motif to interro

135 Collectively, our results identify conserved residues in the membrane-proximal region of CN

136 mmunoelectron microscopy to demonstrate that conserved residues in the MHR are required after assembl

137 Using yeast coa6Delta cells, we show that conserved residues in the motif, including the residue m

138 s X-ray crystal structures, we mutated three conserved residues in the Nef dimer interface (Leu(112),

139 Variants affected highly conserved residues in the Popeye (p.Leu155 and p.Leu217)

140 These amino acid substitutions affect well-conserved residues in the prodomain and in the peptidase

141 Additionally, we found that mutating several conserved residues in the putative client-binding groove

142 esis-based approach, we demonstrate that the conserved residues in the putative cyclin-binding motif

143 that the intermolecular interactions between conserved residues in the RNase domain are required for

144 Substitution of conserved residues in the SAM binding pocket reveals a f

145 the mechanism of Erv14 function, we identify conserved residues in the second transmembrane domain of

146 Mutations of the conserved residues in the WY domain did not affect the s

147 Accordingly, single mutation of specific conserved residues in these motifs, whilst irrelevant in

148 The strictly and strongly conserved residues in this domain cluster in a single ar

149 The presence of several conserved residues in this region together with these st

150 formation by demonstrating that defined non-conserved residues in TM3 and ECL2 of classic claudins c

151 Mutation of highly conserved residues in transcription factors may affect p

152 Mutation of the conserved residues in two members of this family, PpPSR1

153 imer interface and involves several strictly conserved residues, including Arg-60.

154 Mutation of any of the conserved residues increases nuclear accumulation of Htt

155 can be suppressed by phosphorylation of core conserved residues inside the SNARE domain.

156 y defined Munc13-1 C2A domain dimer revealed conserved residues involved in CAPS dimerization.

157 Substitution of conserved residues involved in either metal or nucleotid

158 Moreover, we identified conserved residues involved in the binding of complex ol

159 The third conserved residue is a Cys within the beta-chain (betaCy

160 Conserved residues just past the recoding site are impor

161 electrostatic interactions with clusters of conserved residues, K326, K328, and R147, on actin.

162 2.Pi state, in which D714 interacts with the conserved residue K693, which possibly stimulates Zn(2+)

163 Most changes in the predicted conserved residues K76, R79, G81, and S83 produce no det

164 n amino acid structurally different from the conserved residue leads to the degradation of RT and, in

165 tion of these interaction sites and of other conserved residues leads to decreased DnaB helicase load

166 SAR studies on conserved residues (Leu25, His26, Val29, Pro30, Phe31, P

167 Substituting the ubiquitously conserved residue leucine 29 to alanine in the pore-form

168 A missense substitution of a highly conserved residue likely to affect the interaction of eI

169 Mutations at non-conserved residues lining the putative binding pocket of

170 le PASTA domain or by mutation of individual conserved residues lining the putative ligand-binding su

171 the NS-causing RRAS2 variants affect highly conserved residues localized around the nucleotide bindi

172 The role of eight invariant and highly conserved residues localized to the active site was inve

173 able LQTS-susceptibility gene and involves a conserved residue localizing to the proline, gltamic aci

174 Moreover, W69, a highly conserved residue located at the interface between layer

175 This mutation affects a highly conserved residue located in the second zinc finger doma

176 t PKD1 phosphorylation at Ser(203), a highly conserved residue located within the PKD1 N-terminal dom

177 type specificity because they bind to highly conserved residues located in the channel's central cavi

178 Here we identified highly conserved residues located on this amino acid loop criti

179 Mutations affect conserved residues located within Ca(2+)-binding loops I

180 The role and function of the conserved residue Lys 73 in the catalytic mechanism of c

181 suggesting that Exo1 uses an evolutionarily conserved residue, Lys(185) This residue interacted with

182 Conserved residues map to the unstructured ERS, loops on

183 Mutating these highly conserved residues markedly reduces c-di-GMP binding and

184 lower magnitude compared with CNIH-3, these conserved residues mediate a direct interaction between

185 A pivotal role for the conserved residue N133 is suggested and further supporte

186 genomics and substituted with evolutionarily conserved residues (N251D, A263S, I299L, F387L, I476V, a

187 d to form a new H-bond with another strictly conserved residue, N46(1.50).

188 These results indicate that a highly conserved residue of an ABC transporter plays an importa

189 a single novel missense variant in a highly conserved residue of FLNC (filamin C; p.V2297M).

190 13 that are all phosphorylated at the highly conserved residue of serine 111 (Ser(111)) in response t

191 variant, c.112G>C (p.Gly38Arg), affecting a conserved residue of SLC39A8.

192 el with an RNase H2 AGS mutation in a highly conserved residue of the catalytic subunit, Rnaseh2a(G37

193 while the missense variant affected a highly conserved residue of the homeobox domain, was consistent

194 Previous studies identified a highly conserved residue of the inner domain, W69, as being inv

195 mTOR are phosphorylated on an evolutionarily conserved residue of their ATP-binding domain.

196 rected mutagenesis, we demonstrated that the conserved residues of CBX2 within the intrinsically diso

197 introduced mutations in noncanonical and in conserved residues of either of the two nucleotide bindi

198 nt in soybeans with deleterious mutations at conserved residues of GmSACPD-C.

199 milar studies of mutagenesis of structurally conserved residues of other tryptophylquinone enzymes.

200 Both DNA variants affect highly conserved residues of S1PR2 and are predicted to be dama

201 he APC/C requires its coactivator as well as conserved residues of the E2 and ubiquitin.

202 fferent de novo missense mutations involving conserved residues of the four GSK3 phosphorylation moti

203 The structural analysis unveils the role of conserved residues of the G-patch in the dynamic interac

204 to the identification of coding mutations in conserved residues of the melanocortin 4 receptor (MC4R)

205 dorferi clones containing point mutations in conserved residues of the putative TPR motif of BB0238 d

206 The mutation alters several conserved residues of the third intracellular loop, hamp

207 fied in these individuals localize to highly conserved residues of this WD-40-repeat-containing prote

208 A conformational switch of highly conserved residues on a separate Wnt hairpin might contr

209 eraction interface maps to a patch of highly conserved residues on B', which when mutated render B' i

210 screen, we have identified a group of highly conserved residues on ProQ's NTD as the primary face for

211 -directed mutagenesis to identify a patch of conserved residues on the pentraxin/laminin-neurexin-sex

212 Mutagenesis of conserved residues on two remote surfaces of the NEL dom

213 Additionally, mapping of conserved residues onto the HAstV CP core and spike doma

214 Furthermore, mapping of phylogenetically conserved residues onto the WD40 domain structure reveal

215 ified a missense mutation affecting a highly conserved residue (p.F90L) in the CALM1 gene encoding ca

216 homologous protein models identified highly conserved residues, particularly at the azole binding si

217 long to the Ig-fold superfamily, the sets of conserved residue positions and identities differ betwee

218 l-shift perturbation experiments, identified conserved residues potentially involved in Cu(+) coordin

219 posttranslational hydroxylation of a highly conserved residue (Pro-62) in the small ribosomal protei

220 eta-hairpin with a turn formed by the highly conserved residues Pro76 and Gly77.

221 Conserved residue proline 25 is involved in sequential u

222 Moreover, substitution of His-223, a conserved residue proposed to activate water in other am

223 her with biochemical data, indicate that the conserved residue Q146 in the flexible loop of HIV-1 int

224 Here, we identified two conserved residues (R151, I155) in the syntaxin-1 linker

225 This conserved residue represents a site of TARP action, inde

226 Mutation of a conserved residue required for sialic acid binding by ot

227 ubulavirus RBPs lack many of the stringently conserved residues required for sialic acid recognition

228 Biochemical and structural studies identify conserved residues required for this interaction and tra

229 odel that exploits knowledge of structurally conserved residue-residue interactions in the coiled-coi

230 with genome maintenance proteins identifies conserved residues responsible for Ts OLD ATPase activit

231 her DEG/ENaC channels, and a mutation of one conserved residue (S353A) associated with Ca(2+) block i

232 Furthermore, mutation of the conserved residues S60 and D318 led to alterations in P2

233 hosphorylation by CSNK1A1 on a set of highly conserved residues (S824-S834), followed by rapid dephos

234 l fractions and is not phosphorylated at two conserved residues (Ser(338) or Thr(197)).

235 PHLPP1 dephosphorylated SGT1 at four conserved residues (Ser-17, Ser-249, Ser-289, and Thr-23

236 nction of P-gp, we substituted a group of 14 conserved residues (seven in both TMHs 6 and 12) with al

237 Soybeans carrying Gmsacpd-c mutations at conserved residues showed the highest stearic acid conte

238 ies of the autotransporter Pet show that the conserved residues significantly quicken completion of t

239 nstrate that DoS form sparse networks of non-conserved residues spanning distant regions.

240 anating from the core scaffold often housing conserved residues specific to individual families.

241 cade of interactions predominantly involving conserved residues such as V139, D148, R167 and K155.

242 uence alignments and the locations of highly conserved residues suggest the presence of a dynamic lat

243 ultiple BMC-H paralogs, each with distinctly conserved residues surrounding the pore, which are assum

244 missense mutation (p.Arg2024Gln) in a highly conserved residue that is essential for carbamoyl-phosph

245 enzyme is compared with mutants at Tyr68, a conserved residue that is located behind the reactive su

246 We also identify a conserved residue that is not required for ATPase activi

247 Mutation of a conserved residue that selectively coordinates the putat

248 stent behaviour is conferred by a network of conserved residues that act as hinge and anchor points t

249 We conducted mutagenesis studies on several conserved residues that are considered critical for chlo

250 tures of NavAb, with helix bending involving conserved residues that are critical for slow inactivati

251 he binding affinity of ACK relies on several conserved residues that are critical for stabilizing the

252 V), we show that the GP2 CT contains certain conserved residues that are essential for virus replicat

253 We performed site-directed mutagenesis of conserved residues that are located in exposed regions o

254 favorable interactions involve seven highly conserved residues that can be used to guide further inh

255 Mutations to several highly conserved residues that can take part in metal recogniti

256 We report the importance of conserved residues that contribute to the overall charge

257 and-binding site and bounded at both ends by conserved residues that coordinate to both calcium and l

258 ere used to define the functions of the five conserved residues that define the FakB protein family (

259 ngs, is facilitated by an insertion loop and conserved residues that hold the 3' primer terminus, and

260 d Thg1-tRNA complex structures have revealed conserved residues that interact with anticodon nucleoti

261 onal assays and site-directed mutagenesis of conserved residues to illuminate principles of ligand-de

262 This study investigates the role of a highly conserved residue, tryptophan residue 420, of the viral

263 Mutation of conserved residues typically involved in glucosyltransfe

264 phosphate additionally required active site conserved residues Tyr(40), Asp(181), and Arg(100)and a

265 transmembrane domains are composed of highly conserved residues, underlining their functional relevan

266 rm10 and variants with alterations in highly conserved residues, using crystal structures solved in t

267 f apelin-36 variants in which evolutionarily conserved residues were mutated, and evaluated their abi

268 erm lineage provided evidence that W324 is a conserved residue, whereas the position equivalent to H5

269 onsible encodes the substitution of a highly conserved residue, which lies outside the benzamide-bind

270 nel of the 40S subunit and contacts mRNA via conserved residues whose functional importance was unkno

271 orters and from the analysis of mutations of conserved residues will improve the understanding of the

272 findings identify a discrete pair of highly conserved residues with an essential role for controllin

273 Mutagenesis of conserved residues with potential catalytic function ide

274 stablish that Cys306 of yeast Gpd1, a highly conserved residue within the active site, is the key tar

275 p.Val404Met is novel and occurs at a highly conserved residue within the C-terminal end of the trans

276 ense substitution (c.5560G>A; p.D1854N) at a conserved residue within the catalytic domain.

277 The mutation is at a highly conserved residue within the DNA binding domain.

278 mutation, c.1532G>A (p.Arg511His), alters a conserved residue within the TBC1 domain.

279 ctionally define the roles of several highly-conserved residues within and near the GfTNMT-active sit

280 n stress-dependent phosphorylation of highly conserved residues within its AAD, and that the stimulat

281 omatic NEK9 mutations, each affecting highly conserved residues within its kinase or RCC1 domains, in

282 Mutation of conserved residues within Rrp6 and Mtr4 at the structura

283 ecedented substituents able to interact with conserved residues within the ATP-binding site.

284 and three non-synonymous variants affecting conserved residues within the C terminus of the VAMP2 SN

285 ids from the carboxyl tail, including highly conserved residues within the catalytic domain, plus a c

286 tructural and mutational analyses identified conserved residues within the CCD of ATG16L1 that mediat

287 in sequences preceding the first C of highly conserved residues within the CX5C or CX3H regions or wi

288 al of the C-tail alone or mutation of highly conserved residues within the domain still allows signif

289 lly characterized the functional roles of 12 conserved residues within the GP2 CT in GP processing, t

290 ractions with ubiquitin may have equivalent, conserved residues within the H(abc) domain of Stx3.

291 We identify conserved residues within the LRRK2 ankyrin domain that

292 after the SP (HRASP), glycosylation and the conserved residues within the N-terminus in GLP-1R traff

293 hermore, site-directed mutagenesis of highly conserved residues within the PepSY domains resulted in

294 We show that mutation of conserved residues within the Rcf1 QRRQ motif alters the

295 In vitro Pkc1 phosphorylates conserved residues within the RING-H2 domains of the sca

296 the effects of alanine substitutions at many conserved residues within the SSP on viral replication i

297 d a systematic mutagenesis at the flavivirus conserved residues within the TMDs of NS2B.

298 We conclude that, although the majority of conserved residues within the TZF domain of TTP are requ

299 lographic and mutational studies reveal that conserved residues within the UBN1-HRD and H3.3 G90 as k

300 Site-specific mutational analyses of the conserved residues within WRDPLVDID indicated that Trp-6