ライフサイエンスコーパス: copurification

1 y their interaction in vitro by coexpression/copurification.

2 Using copurification analysis and far-Western blotting, we fou

3 xistence of heterotrimers is implicated from copurification and crosslinking studies carried out in v

4 Copurification and Cys matching analyses suggest that se

5 hoproteomics and bioinformatics, followed by copurification and dephosphorylation assays.

6 d proteasome-interacting proteins by reverse copurification and immunoblotting experiments with and w

7 Based on their copurification and intracellular distribution, vaults ma

8 Copurification and pull-down experiments showed that the

9 Copurification and two-hybrid studies showed that Scm3 a

10 ability to interact with UL5 as indicated by copurification and with UL8 as indicated by a supershift

11 Chemical cross-linking in vivo and copurification approaches established that YknX interact

12 Using both yeast two-hybrid and copurification approaches, we identified the protein-pro

13 and EpsL by functional characterization and copurification as well as coimmunoprecipitation.

14 We developed a novel Escherichia coli copurification assay to map the domain on the Drosophila

15 Using the copurification assay, we scanned this 28-amino-acid inte

16 A combination of in vitro (copurification assays) and in vivo (bimolecular fluoresc

17 to the tail domain of KHC in two-hybrid and copurification assays, indicating that kinesin and UNC-7

18 By two-hybrid and copurification assays, the DM3 mutant was found to be im

19 itro glutathione S-transferase pulldown, and copurification assays.

20 This cross-linking event enables subsequent copurification by a tag on the recombinant protein.

21 en the full-length proteins, suggesting that copurification can serve as a surrogate measure of inter

22 Coimmunoprecipitation and chromatographic copurification data suggest that pVHL-Cul2 complexes exi

23 Copurification experiments demonstrated that Ssa1p bound

24 required for cytochrome bd oxidase activity, copurification experiments indicate that CydX interacts

25 Copurification experiments indicated that the interactio

26 e DNA ratio for the two subunits was varied, copurification experiments indicated that the subunit st

27 modulin and TMEM16A could not be detected in copurification experiments or in functional assays.

28 Copurification experiments suggested that the MBD binds

29 To test this prediction, we used copurification experiments to identify five distinct and

30 h a WD40 repeat-containing protein, Cdh1, by copurification experiments.

31 A56/K2 and the EFC was demonstrated by their copurification from detergent-treated lysates of infecte

32 hypothesis, we describe herein the specific copurification from human placenta of 46- and 14-kDa pro

33 Copurification is enhanced by HTT polyQ expansion and re

34 Here, a copurification-mass spectrometry approach was taken usin

35 oped in H. volcanii, allowing the successful copurification of (i) Hv7Sh with a histidine-tagged Hv54

36 a-sheet conformation can in part explain the copurification of A beta and apoE from AD amyloid plaque

37 These results demonstrate copurification of a channel-associated G-protein that is

38 tors via a FLAG-tagged G subunit resulted in copurification of a Myc-tagged G subunit, implying more

39 Such an interaction was established by the copurification of A16 and G9 from infected cells under c

40 n vivo Reg1-Bmh interaction was confirmed by copurification of Bmh proteins with HA(3)-TAP-tagged Reg

41 t the N terminus, I5A, significantly reduced copurification of both ACBD3 and PI4KIIIbeta.

42 The copurification of calreticulin and endomannosidase from

43 rophyll recycling in a Ycf39-null mutant and copurification of chlorophyll synthase and unassembled D

44 s support this conclusion as demonstrated by copurification of coexpressed WT and L103A proteins in E

45 on affecting the T-loop of NifI(2) prevented copurification of dinitrogenase but did not affect copur

46 Copurification of eNOS with mitochondria was observed in

47 The identification was confirmed by copurification of enzyme activities, by similarities in

48 prevent deubiquitination after lysis or the copurification of interacting cofactors, this procedure

49 found that gE/gI are necessary for efficient copurification of KIF1A with Us9, especially at early ti

50 complex by using chemical cross-linking and copurification of LcrG and LcrV.

51 Copurification of MdtB and MdtC under these conditions s

52 rification of His-tagged NifI(2) resulted in copurification of NifI(1) and the dinitrogenase subunits

53 fication of dinitrogenase but did not affect copurification of NifI(1).

54 sion and affinity purification revealed that copurification of PI4KIIIbeta could be eliminated by mut

55 odepletion of the moesin kinase activity and copurification of PKC-theta with the enzymic activity.

56 hBH and UBC9 interacted in vivo by affinity copurification of proteins overexpressed in mammalian ce

57 nd absence of intact capsids and by affinity copurification of pU(L)17 and VP13/14 from lysates of ce

58 Interestingly, we find copurification of Rad2 protein with TFIIH, such that TFI

59 Notably, copurification of RecA with DinB is somewhat enhanced in

60 Copurification of subunits II and III with aposubunit I

61 Copurification of the Aac2 protein with the TIM23 machin

62 the alpha(1)beta-proteasome resulted in the copurification of the alpha(2) protein with the alpha(1)

63 This strong association confers an effective copurification of the antigen with the starch fraction o

64 rnover of s-ODN in the brain, we studied the copurification of the immunoreactivity of biotin with bi

65 n between PKI and pPKR is indicated by their copurification on dsRNA agarose, despite evidence showin

66 dence for an interaction was provided by the copurification on immunoglobulin G-Sepharose of Nip7p wi

67 Copurification studies also identified physical interact

68 Copurification studies show that although Cpc2 and Ran1

69 Copurification studies show that unphospho-PtsN specific

70 Such copurification studies will provide insight into the sig

71 n vitro binding, as well as coexpression and copurification studies, gave evidence of CheA/CheS compl

72 We found using affinity copurifications that Ddi2 binds Ub conjugates through it

73 and ColE1 by using cross-linking followed by copurification via histidine-tagged TolC.

74 y 600 kDa composed of six polypeptides whose copurification was completely dependent on the polyhisti

75 Here, using AlphaFold2 modeling and copurification, we show how a conserved signature motif

76 ing the in situ proximity ligation assay and copurifications, we show that the binding of Nck to the

77 On the basis of its copurification with an SRB-containing RNA polymerase II

78 to identify the direct substrates of ClpC by copurification with ClpC(trap) followed by gel electroph

79 itin conjugation, polyubiquitin binding, and copurification with deubiquitinating enzymes.

80 combinant BHMT-2 that has been stabilized by copurification with human recombinant BHMT.

81 ith the cytosolic fraction, as documented by copurification with lactate dehydrogenase activity.

82 idoreductase activity of both enzymes, their copurification with menaquinone, and the distinctive det

83 o controls, and EXOSC4-L187P shows decreased copurification with other RNA exosome subunits.

84 We isolated Tic56 by copurification with Tandem Affinity Purification-tagged

85 egulator, a protein called CoaX, through its copurification with the depupylase Dop.

86 ed protein because of its colocalization and copurification with the IF proteins desmin and vimentin

87 s as polymerase complex components (based on copurification with the polymerase activity and by coimm

88 n with the TBSV p33 replication protein, its copurification with the tombusvirus replicase, and its p

89 For RNA polymerase, reduced copurification with Tnp is observed in extracts from a t

90 Vimentin copurification with UPase was confirmed using both Weste

91 ochore localization, similar phenotypes, and copurification with xNdc80 and xNuf2.