ライフサイエンスコーパス: coronin

1 an ezrin-radixin-moesin-family protein, and coronin.

2 but no genetic interaction with deletion of coronin.

3 hieved by cofilin, assisted by Aip1/Wdr1 and coronins.

4 t actin confinement is facilitated by type I coronins.

5 e generated coronin 1-/- mice and found that coronin 1 exerted an inhibitory effect on cellular stead

6 ive role of the WD repeat containing protein coronin 1 in the maintenance of naive T cells in periphe

7 We show that coronin 1 is dispensable for thymocyte survival and deve

8 Interestingly, disruption of coronin 1 promotes allograft tolerance while immunity to

9 thway driven by the immunoregulatory protein coronin 1 that regulates the phosphodiesterase/cAMP path

10 thway driven by the immunoregulatory protein coronin 1 that regulates the phosphodiesterase/cyclic ad

11 Whereas coronin 1 was required for chemokine-mediated migration,

12 We found that mice deficient in coronin 1, a regulator of naive T cell homeostasis, full

13 We generated coronin 1-/- mice and found that coronin 1 exerted an in

14 Mechanistically, coronin 1-deficiency increased cyclic adenosine monophos

15 Importantly, coronin 1-deficient mice possessed comparable levels of

16 ation of this pathway or a prior transfer of coronin 1-deficient T cells actively suppressed allograf

17 est the existence of a hitherto unrecognized coronin 1-dependent decision switch early during life th

18 These results define a coronin 1-dependent regulatory axis in T cells important

19 cell survival was found to be independent of coronin 1.

20 cruitment of a new effector protein known as Coronin-1 (also called Coro1a).

21 In contrast, the presence of Coronin-1 (wild-type neurons) suppresses but does not ha

22 These results establish Coronin-1 as an essential component of a feedback loop t

23 at periods before and after NGF-TrkA-induced Coronin-1 expression (and likely other factors) defines

24 ogether with previous work demonstrating the Coronin-1 expression is NGF dependent, this work suggest

25 Beyond influencing endosomal trafficking, Coronin-1 is also required for several NGF-TrkA-dependen

26 ysosomes sixfold to tenfold faster than when Coronin-1 is intact.

27 In the absence of Coronin-1 we find that NGF-TrkA-PI3K signaling drives ro

28 e the role of the NGF-TrkA effector protein, Coronin-1, on postganglionic sympathetic neuron final ta

29 In the absence of Coronin-1, the NGF-TrkA signaling endosome fuses to lyso

30 ppression in axon growth behaviors is due to Coronin-1-dependent calcium release via PLC-gamma1 signa

31 We also define a new Coronin-1-dependent trafficking event in which signaling

32 (2019) identify a coronin-1-PDE4-cAMP axis that, when perturbed, results i

33 Here, we identify coronin 1A (Coro1A) as a novel regulator of beta2 integr

34 be a novel role of the actin binding protein coronin 1A (Coro1A) in neuronal morphogenesis, where it

35 Coronin 1A (Coro1A) is involved in cytoskeletal and sign

36 Coronin 1A (Coro1A) is the hematopoietic-specific member

37 lack this posttranslational modification of coronin 1a and exhibit defective TCR-induced actin polar

38 Thus, the phenotype of T cell-specific Coronin 1a deletion resembles the phenotype observed wit

39 subsets were not affected by the deletion of Coronin 1a Furthermore, T cell-specific Coronin 1a knock

40 Our findings establish a function for coronin 1A in T cell egress, identify a surface of coron

41 Nevertheless, because Coronin 1A is strongly expressed in all leukocyte subset

42 Studies analyzing complete Coronin 1a knock-out mice have shown that this molecule

43 n of Coronin 1a Furthermore, T cell-specific Coronin 1a knock-out mice were largely resistant to the

44 type observed with conventional (whole body) Coronin 1a knock-out mice.

45 uestion, we have generated a T cell-specific Coronin 1a knock-out mouse (Coro1a(fl/fl) x Cd4[Cre]).

46 The discovery of another coronin 1A mutant during an N-ethyl-N-nitrosourea-mutage

47 iated phosphorylation of the actin modulator coronin 1a on threonine 418.

48 Deletion of Coronin 1A specifically in T cells led to a strong reduc

49 c acid at position 26 in the actin regulator coronin 1A that enhanced its inhibition of the actin reg

50 he negative regulator of F-actin generation, Coronin 1A, at the center of the T cell interface with t

51 depletion of the main coronin of leukocytes, coronin 1A, fully restores the cortical location of F-ac

52 of the predominant T cell-intrinsic role of Coronin 1A.

53 ared with Cd4[Cre] mice expressing wild-type Coronin 1A.

54 attributed to a T cell-intrinsic function of Coronin 1A.

55 rited missense mutation in the gene encoding Coronin-1A (CORO1A) in the 3 siblings.

56 Coronin-1A (CORO1A) is a regulator of actin dynamics imp

57 Coronin-1A (Coro1A) was identified as a differentially e

58 ree-dimensional reconstruction, we show that coronin-1A binds to three protomers in F-actin simultane

59 In this issue of Immunity, the Coronin-1A gene Coro1a, which regulates cytoskeletal str

60 mutation of the filamentous actin-inhibiting Coronin-1A gene.

61 Our results demonstrate that Coronin-1A is required for the development of systemic l

62 tion in patients associated with hypomorphic Coronin-1A mutation.

63 ctin may participate in the interaction with coronin-1A.

64 Coronin 1B (Coro1B) and 1C (Coro1C) were both found to b

65 ore, we identified the actin-binding protein Coronin 1B (Coro1B) as a substrate of USP45.

66 Coronin 1B also directs SSH1L to lamellipodia where SSH1

67 Coronin 1b and Rab13 proteins were expressed in cultured

68 Finally, Coronin 1b and Rab13 were induced in the injured spinal

69 xperiments show that the interaction between Coronin 1B and the Arp2/3 complex is regulated by protei

70 esis, we have identified serine 2 (Ser-2) on Coronin 1B as the major residue phosphorylated by PKC in

71 hibition is attenuated by phosphorylation of Coronin 1B at Serine 2, a site targeted by SSH1L.

72 e assay to identify cells with an absence of Coronin 1B brought about by the successful infection of

73 We report that Coronin 1B disassembles Arp2/3-containing actin filament

74 Accordingly, depleting Coronin 1B increases phospho-Cofilin levels, and alters

75 Coronin 1B inhibits filament nucleation by Arp2/3 comple

76 We report that Coronin 1B interacts simultaneously with Arp2/3 complex

77 Our results show that Coronin 1B is a ubiquitously expressed member of the mam

78 go apoptosis within an epithelial monolayer, coronin 1B is also recruited to the junctional cortex at

79 Coronin 1B is phosphorylated by PKC both in vitro and in

80 In the absence of coronin 1B or cofilin, Tpm1.8/9 protein levels are reduc

81 Moreover, RNAi gene silencing for Coronin 1b or Rab13 in NGF-treated PC-12 cells markedly

82 Furthermore, Coronin 1B phosphorylation is responsible for a signific

83 These data demonstrate that Coronin 1B regulates leading edge dynamics and cell moti

84 We conclude that Coronin 1B replaces the Arp2/3 complex at actin filament

85 Rat2 fibroblasts expressing the Coronin 1B S2A mutant show enhanced ruffling in response

86 Cells expressing the Coronin 1B S2D mutant have attenuated PMA-induced ruffli

87 Coronin 1B targets actin branches in a manner that is mu

88 hanges could be rescued by overexpression of Coronin 1B together with a constitutively active Cofilin

89 y, clonal cell lines with siRNA knockdown of Coronin 1B were generated using the arrays and compared

90 We conclude that Coronin 1B's coordination of filament formation by Arp2/

91 preferentially interacts with phosphorylated Coronin 1B, allowing it to complex with Slingshot phosph

92 conclude that WISp39 associates with Hsp90, Coronin 1B, and SSH to regulate Cofilin activation and A

93 WISp39 binds Coronin 1B, known to regulate the Arp2/3 complex and Cof

94 small GTPase Rab13 and actin-binding protein Coronin 1b, showed significantly increased mRNA expressi

95 cks the actin-Arp2/3 interaction [4, 5], and coronin 1B, which acts by directing SSH1L to the lamelli

96 Reorganization requires coronin 1B, which is recruited to junctions by E-cadheri

97 , a phenotype consistent with cortactin- and coronin 1B-deficient cells [2, 7].

98 n combined with actin disassembly co-factors Coronin-1B and actin-interacting protein 1 (AIP1), and t

99 s the inhibitory effect of nonphosphorylated coronin-1b on actin nucleation.

100 In addition, METH-induced coronin-1b phosphorylation diminishes the inhibitory eff

101 SMTNL2 binds to coronin-1B through its N-terminal coiled-coil region and

102 Although coronin-1B-mediated actin dynamics are required for earl

103 s its functions partly through inhibition of coronin-1B.

104 Loss of Coronin 1C in this model increases both primary tumor gr

105 Coronin 1C is overexpressed in multiple tumors, leading

106 Here, we combined a conditional knockout of Coronin 1C with a genetically engineered mouse model of

107 Our observations demonstrate that coronin 1C, cortactin, and MICAL2 act together to promot

108 ecific mechanism of EV release, regulated by Coronin 1C, that contributes to the high rates of metast

109 Coronin 1C-null cells isolated from this model are more

110 tatic lesions in orthotopic transplants than Coronin 1C-reexpressing cells due to the shedding of ext

111 , which is recruited to the actin network by coronin 1C.

112 We discover that both coronin-1C and caveolin retrieve Rac1 from similar locat

113 In the absence of coronin-1C, the effect of caveolin-mediated endocytosis,

114 We find that coronin-1C-mediated extraction, which is responsible for

115 Unlike constitutive coronin-1C-mediated trafficking, caveolin-mediated Rac1

116 Here we provide evidence that coronin 2A (CORO2A), a component of the NCoR complex of

117 al microtubules become enriched in actin and coronin [6].

118 degradable, K33-linked polyubiquitination on coronin 7 (Crn7), which facilitates Crn7 targeting to TG

119 the TGN through K33-linked ubiquitination of coronin 7.

120 ole for the evolutionarily conserved protein coronin A in the initiation of the cAMP response.

121 These results suggest that coronin A is dispensable for cAMP sensing, chemotaxis, a

122 On starvation, coronin A-deficient cells failed to up-regulate the expr

123 Of importance, external addition of cAMP to coronin A-deficient cells resulted in normal chemotaxis

124 ng a functional cAMP relay in the absence of coronin A.

125 Coronins, a family of seven proteins in humans, inhibit

126 tions, some phagosomes showed persistence of coronin about the surface of the compartment, but coloca

127 We show that coronin accelerates the release of Pi from actin filamen

128 nin isoforms suggests that the nature of the coronin-actin association may be similar in other family

129 proteins (cofilin, GMF, twinfilin, Srv2/CAP, coronin, AIP1, capping protein, and profilin) work in co

130 We find that the cooperative binding of coronin allosterically promotes inorganic phosphate rele

131 Depletion of type I coronins allows actin to extend along the length of the

132 s that express GFP-tagged fusion proteins of Coronin and actin-interacting protein 1, as well as knoc

133 ein 1, as well as knockout mutants that lack Coronin and actin-interacting protein 1.

134 Together, coronin and Aip1 lower the amount of cofilin required to

135 Coronin and Aip1 promote cofilin-mediated actin filament

136 n assays revealed an association of Ndm with coronin and F-actin.

137 ve inhibitory effects on Arp2/3 complex with Coronin and GMF.

138 s, a localization pattern that is similar to coronin and partly dependent on RacE.

139 of the biochemical and cellular functions of coronin and that the beta-propeller domain mediates addi

140 xture of actin disassembly factors (cofilin, coronin, and actin-interacting protein 1 is sufficient t

141 sembly of single actin filaments by cofilin, coronin, and actin-interacting protein 1, a purified pro

142 or by the collaborative effects of Cofilin, Coronin, and AIP1 (CCA).

143 el proposed that the combination of cofilin, coronin, and Aip1 disassembled filaments in bursts.

144 The cooperative activities of cofilin, coronin, and Aip1 should provide a biochemical basis for

145 ith the Dictyostelium actin-binding protein, coronin, and approximately 67% homology with the previou

146 itory ligands: glia maturation factor (GMF), Coronin, and Arpin.

147 osin II and globally distributed dynacortin, coronin, and RacE.

148 Coronins are a conserved family of WD repeat-containing,

149 Coronins are conserved actin-binding proteins that regul

150 Together, our observations reveal that coronins are critical for proper turnover of branched ac

151 Coronins are evolutionarily conserved proteins that were

152 Coronins are F-actin-binding proteins that are involved,

153 Although coronins are known to play a role in controlling actin d

154 Coronin arrives last as all other components disperse up

155 chemical fractionation, we identify Aip1 and coronin as two proteins present in thymus extract that f

156 mutational analysis of surfaces on the yeast coronin beta-propeller domain, which binds to F-actin an

157 to pointed ends, while not interfering with Coronin binding to filament sides.

158 and Rac-interactive binding motif (CRIB) of coronin binds to Rho GTPases with a preference for GDP-l

159 Such a mode of binding explains how coronin can stabilize actin filaments in vitro.

160 It is rescued by wild-type coronin, whereas coronin carrying a mutated Cdc42- and Rac-interactive bi

161 tin networks have remained elusive; however, Coronin, Cofilin and AIP1 have been implicated in this p

162 The conserved actin-binding proteins coronin, cofilin, and actin-interacting protein 1 (AIP1)

163 e uncover the concerted molecular actions of coronin, cofilin, and AIP1 that lead to actin filament a

164 t is inconsistent with the current models of coronin-cofilin functional interaction.

165 tional knockout cell line for two ubiquitous coronins, Coro1B and Coro1C.

166 ve investigated the mechanism by which yeast coronin (Crn1) enhances F-actin turnover.

167 Here, we show that yeast coronin (Crn1) makes a unique contribution to this proce

168 of Arp2/3 complex, Saccharomyces cerevisiae coronin (Crn1), enhances Gmf1-mediated debranching by 8-

169 tailed biochemical analysis of budding yeast coronin, Crn1, and found that it not only inhibits Arp2/

170 aphy, we coisolated actin and a homologue of coronin, Crn1p, from Saccharomyces cerevisiae cell extra

171 ults in increased 1evels of activated Rac in coronin-deficient Dictyostelium cells (corA(-)), which i

172 and cell adhesion components (e.g., catenin, coronin, dystrobrevin, and syndecan), consistent with it

173 In other cell types, coronins exert their effects on lamellipodia dynamics by

174 ich binds to F-actin and is conserved in all coronin family members.

175 is the hematopoietic-specific member of the Coronin family of actin regulators that promote F-actin

176 this protein, POD-1, as a new member of the coronin family of actin-binding proteins.

177 Cofilin then displaces coronin from the filament via a strand-restricted cooper

178 An understanding of coronin function has been hampered by the absence of any

179 equired for continued cortical growth, while Coronin functions in both growth and intensity and is re

180 iquitously expressed member of the mammalian Coronin gene family that co-localizes with the Arp2/3 co

181 FP-dynacortin and the actin bundling protein coronin-GFP are seen to concentrate in highly dynamic co

182 We therefore examined whether yeast lacking coronin had defects in the microtubule cytoskeleton.

183 nts that lack coronin, yeast strains lacking coronin had no detectable defects in actin-based process

184 rved disassembly-promoting factors including coronin have remained more obscure.

185 that contains sequences (not found in other coronins) homologous to the microtubule binding region o

186 Despite emerging roles for coronin in a range of physiological processes and diseas

187 Furthermore, GMF synergized with Coronin in inhibiting actin nucleation by Arp2/3 complex

188 twist in the interplay of cofilin, Wdr1, and coronin in regulating F-actin dynamics.

189 be the identification of a single homolog of coronin in Saccharomyces cerevisiae, which we show local

190 However, the existence and role of coronins in vascular smooth muscle cell (VSMC) migration

191 Lastly, we demonstrate that the loss of coronins increases accompanied by an increase in cellula

192 n 1A in T cell egress, identify a surface of coronin involved in Arp2/3 regulation and demonstrate th

193 er, these results suggest that S. cerevisiae coronin is a component of the actin cytoskeleton that ma

194 Coronin is a conserved actin-binding protein that co-fun

195 Coronin is a highly conserved actin-associated protein t

196 More recent studies have revealed that coronin is involved in actin-based motility, cytokinesis

197 y during the initiation of the response, and coronin is recruited as the actin filaments are disassem

198 icating that activation of Arp2/3 complex by coronin is required for normal actin dynamics in vivo.

199 nt species and in all three major classes of coronin isoforms suggests that the nature of the coronin

200 with the previously cloned human and bovine coronin-like homologue, p57.

201 ing proteins and the F-actin-binding protein coronin localize to its leading edge, but in an shk1 nul

202 Coronins make up a large class of actin-binding proteins

203 The coronin mutant and the myoA/B double myosin I mutant wer

204 n mouse macrophages, a defined D. discoideum coronin mutant was analyzed.

205 ud neck, failed to reveal any defects in the coronin mutant.

206 Coronin null cells have altered lamellipodial protrusion

207 nt with coronins playing a pro-cofilin role, coronin null cells have increased F-actin levels.

208 rprisingly, excessive cofilin accumulates in coronin null lamellipodia, a result that is inconsistent

209 Strikingly, depletion of the main coronin of leukocytes, coronin 1A, fully restores the co

210 wed normal actin binding but failed to cause coronin overexpression defects.

211 ion to actin structures in vivo, and loss of coronin overexpression growth defects.

212 However, consistent with coronins playing a pro-cofilin role, coronin null cells

213 s (2016) show that the actin binding protein Coronin plays a critical role in actin cytoskeleton reor

214 We conclude that, analogous to coronin, POD-1 plays an important role in intracellular

215 We conclude that coronin polarizes the spatial distribution and activity

216 with the N-terminal beta-propeller domain of Coronin positioned near the p35/ARPC2 subunit of Arp2/3

217 Coronin promoted a standard (previously described) open

218 t study was to define the mechanism by which coronins regulate platelet-derived growth factor (PDGF)-

219 Surprisingly, the absence of coronin resulted in enhanced intracellular replication o

220 ude that in D. discoideum factors other than coronin support intracellular replication of M. marinum.

221 We propose that coronin through its affinity for GDP-Rac regulates the a

222 Coronin was originally identified as a cortical protein

223 It is rescued by wild-type coronin, whereas coronin carrying a mutated Cdc42- and R

224 To test the role of host coronin, which was previously hypothesized to positively

225 These coronins, which strongly co-localize with Arp2/3-branche

226 derstanding of the molecular interactions of coronin with actin and other binding partners has been l

227 Unlike Dictyostelium mutants that lack coronin, yeast strains lacking coronin had no detectable