ライフサイエンスコーパス: corrinoid

1 in the presence of distinct B(1)(2) analogs (corrinoids).

2 12 but does so without adenosylation of this corrinoid.

3 eine or dithiothreitol, resulting in a Co(I) corrinoid.

4 ltransferases catalyze the activation of the corrinoid.

5 parameters that are unprecedented for Co(2+)corrinoids.

6 radation, debromination by Zn(0) and reduced corrinoids.

7 Several known enzymes adenosylate corrinoids.

8 5.0-5.3 for PceA enzyme and 3.7-4.5 for the corrinoids.

9 ll structure but a different selectivity for corrinoids.

10 grow on acetate in the absence of exogenous corrinoids.

11 erent compared to dehalogenation mediated by corrinoids (4.6-7.0).

12 --> pi transitions, which dominate the Co(2+)corrinoid Abs spectra, are essentially insulated from pe

13 potassium borohydride in in vitro assays for corrinoid adenosylation catalyzed by the ATP:co(I)rrinoi

14 The specificity of the ATP:corrinoid adenosyltransferase (CobA) enzyme of Salmonell

15 d as a substrate for EutT, the ATP-dependent corrinoid adenosyltransferase and for the EutA ethanolam

16 n the active site of PduO-type ATP-dependent corrinoid adenosyltransferase enzymes.

17 The PduO-type ATP:corrinoid adenosyltransferase from Lactobacillus reuteri

18 mensional crystal structure of the PduO-type corrinoid adenosyltransferase from Lactobacillus reuteri

19 The functional assignment of LrPduO as a corrinoid adenosyltransferase was confirmed by in vivo a

20 ially replaced by the cobA gene (a known ATP:corrinoid adenosyltransferase) but that optimal growth o

21 ATP:Corrinoid adenosyltransferases (ACAs) catalyze the trans

22 Three distinct families of ATP:corrinoid adenosyltransferases (ACATs) exist that are ca

23 robic conditions, but it can form "complete" corrinoids aerobically by importing an "incomplete" corr

24 as well as the exchange and modification of corrinoids among community members have not been well st

25 view reaction mechanisms and the role of the corrinoid and Fe-S cluster cofactors and discuss physiol

26 mes of such anaerobic catabolic pathways are corrinoid and Fe-S cluster-containing, membrane-associat

27 Reductive dehalogenases are corrinoid and iron-sulfur cluster-containing enzymes tha

28 wed a DMB-off/His-on interaction between the corrinoid and the enzyme, whose catalytic efficiency was

29 ssesses a high-affinity transport system for corrinoids and that this archaeon can synthesize cobamid

30 be the interaction between Co(3+)- and Co(2+)corrinoids and the enzyme active site.

31 for dibromoelimination pathway with reduced corrinoids and Zn(0) particles; EDB biodegradation by An

32 Cobalamin and other corrinoids are essential cofactors for many organisms.

33 in vivo, nonadenosylated cobalamin and other corrinoids are maintained as co(II)rrinoids by reduced f

34 Corrinoids are the vitamin B(12) (cobalamin) family of o

35 s that support Dehalococcoides growth (e.g., corrinoid assembly, and production of intermediates requ

36 The corrinoid B(12) is synthesized only by prokaryotes yet i

37 The methylation of Hg(II) (SCH3 )2 by corrinoid-based methyl donors proceeds in a concerted ma

38 ntial variations in ATP binding and probably corrinoid binding between CobA(Se) and CobA(Mm) enzymes.

39 ons of these homologies for the mechanism of corrinoid binding by proteins involved in methylotrophic

40 MtmC possesses the corrinoid binding motif found in corrinoid proteins invo

41 The structures define the corrinoid binding site and provide visual evidence for a

42 n y-butyrobetaine-grown cells: MtyB, MtqC (a corrinoid-binding protein), and MtqA (a corrinoid:tetrah

43 hains of the corrin ring and is conserved in corrinoid-binding proteins of other species.

44 Knowledge about corrinoid biology at each scale informs and reinforces a

45 harboring reductive dehalogenase and de novo corrinoid biosynthesis operons, functions critical to or

46 MtbB1 methylated either corrinoid bound to MtbC or free cob(I)alamin with dimeth

47 se in which MtsA mediates the methylation of corrinoid bound to MtsB with dimethylsulfide and subsequ

48 The endogenous methylated corrinoid bound to the beta subunit of the 480-kDa prote

49 -visible spectra showed that enzyme Fe-S and corrinoid centers were already fully reduced at levels o

50 PduO can in fact also utilize the incomplete corrinoid Co (1+)cobinamide (Co (1+)Cbi) as an alternati

51 pha-R, allowing conversion of the incomplete corrinoid cobinamide (Cbi) into AdoCbl.

52 d freshwater habitats require for growth the corrinoid cofactor B12, which is synthesized de novo onl

53 ansferase II which remains firmly bound to a corrinoid cofactor binding subunit during isolation.

54 ine methyltransferase (MMAMT) methylates the corrinoid cofactor bound to a second polypeptide, monome

55 The role of the corrinoid cofactor in reductive dehalogenation catalysis

56 iguously establish the binding scheme of the corrinoid cofactor in the CFeSP, we have combined resona

57 s in equimolar concentrations which bind one corrinoid cofactor per alphabeta dimer.

58 l carrier and an electron donor required for corrinoid cofactor reduction, respectively.

59 small subunit (33 kDa) contains cobalt in a corrinoid cofactor, and the large subunit (55 kDa) conta

60 , iron, acid-labile sulfide, and cobalt in a corrinoid cofactor.

61 bound one zinc atom per polypeptide, but no corrinoid cofactor.

62 ate, and DMS and MMPA could remethylate, the corrinoid cofactor.

63 MtbC binds 1.0 mol of corrinoid cofactor/mol of 24-kDa polypeptide and stimula

64 Also, dcpA, a gene described to encode a corrinoid-containing 1,2-DCP reductive dehalogenase was

65 idazolylcobamide methyltransferase (MtaB), a corrinoid-containing methyl-accepting protein (MtaC) and

66 V-visible spectrum was typical of methylated corrinoid-containing proteins, with absorbance maxima at

67 The Co(I) corrinoid could be remethylated by methyl iodide, and th

68 h corrinoid-dependent enzymes do not produce corrinoids de novo, and instead must acquire corrinoids

69 aerobic conditions, S. enterica performs the corrinoid-dependent degradation of ethanolamine if given

70 The majority of microbes with corrinoid-dependent enzymes do not produce corrinoids de

71 can use pseudo-vitamin B12 for all of their corrinoid-dependent enzymes.

72 terial genomes, often located near genes for corrinoid-dependent methyltransferases.

73 wever, the profile of corrinoids produced in corrinoid-dependent microbial communities, as well as th

74 es and, together with two recently described corrinoid-dependent reductive dehalogenases, constitute

75 hromatography tandem mass spectrometry-based corrinoid detection method to examine relationships amon

76 computational methodology for studying Co(2+)corrinoid/enzyme active site interactions is demonstrate

77 logenetic groups in corrinoid production and corrinoid exchange within microbial communities.

78 ate requirement for benzimidazole-containing corrinoids for trichloroethene respiration.

79 lamin and to salvage incomplete and complete corrinoids from the environment of this bacterium.

80 In vitro experiments with reduced corrinoid (i.e., vitamin B(12)) mediated reductive dechl

81 ould be initiated only with the enzyme-bound corrinoid in the methylated state.

82 re]Cba) and cobalamin were the most abundant corrinoids in the communities.

83 umably due to the absence of bound, inactive corrinoids in the recombinant enzyme.

84 rate mechanisms to capture and differentiate corrinoids in vivo and that apparent redundancies observ

85 isms (e.g., production of H(2), acetate, and corrinoids) in PCB-contaminated sediments, metagenomic a

86 e signature residues involved in binding the corrinoid, including a histidyl residue which ligates co

87 Our data reveal that while Co(3+)corrinoids interact only weakly with CobA, Co(2+)corrino

88 olves the formation of an "activated" Co (2+)corrinoid intermediate that lacks any significant axial

89 Two new cobalt corrinoid intermediates, cobalt-precorrin 5A and cobalt-

90 In corrinoid iron-sulfur methyltransferases from acetogenic

91 hat nitrate also decreased the levels of the corrinoid iron-sulfur protein (60%) and methyltransferas

92 actions between methyltransferase (MeTr) and corrinoid iron-sulfur protein (CFeSP) are required for t

93 The corrinoid iron-sulfur protein (CFeSP) from Clostridium t

94 Here we have studied the axial ligation of a corrinoid iron-sulfur protein (CFeSP) that plays a key r

95 thyl-cobalt(III) species on one protein (the corrinoid iron-sulfur protein (CFeSP)) to a nickel iron-

96 CoA synthesis from CoA, CO, and a methylated corrinoid iron-sulfur protein (CFeSP).

97 thyl group from the CH(3)-Co(3+) site in the corrinoid iron-sulfur protein (CFeSP).

98 odels and experiments identified T7RdhA as a corrinoid iron-sulfur protein (CoFeSP) which uses a norp

99 The gamma and delta subunits constitute a corrinoid iron-sulfur protein that is involved in the tr

100 CoA synthesis from CO, CoA, and a methylated corrinoid iron-sulfur protein, which acts as a methyl do

101 B(12)-dependent methyl transfer, namely the corrinoid iron-sulphur protein and its methyltransferase

102 uctants), CoA, and a methyl group bound to a corrinoid-iron sulfur protein (CoFeSP).

103 he methyltetrahydrofolate (CH(3)-H(4)folate) corrinoid-iron-sulfur protein (CFeSP) methyltransferase

104 CO, CoA, and a methyl group donated from the corrinoid-iron-sulfur protein (CoFeSP).

105 group is transferred onto the enzyme from a corrinoid-iron-sulfur protein (CoFeSP).

106 The corrinoid-iron/sulfur protein (CoFeSP) operates within t

107 The corrinoid/iron-sulfur component contained 0.7 Co, 0.7 fa

108 ible spectroscopy indicated that the reduced corrinoid/iron-sulfur component could be methylated with

109 oxidized CO and transferred electrons to the corrinoid/iron-sulfur component, reducing the iron-sulfu

110 4Fe-4S cluster previously identified in the corrinoid/iron-sulfur enzyme by electron paramagnetic re

111 x from Methanosarcina thermophila contains a corrinoid/iron-sulfur enzyme composed of two subunits (d

112 encoding the beta and alpha subunits of the corrinoid/iron-sulfur enzyme from Clostridium thermoacet

113 hermoaceticum catalyzes the methylation of a corrinoid/iron-sulfur protein (C/Fe-SP) by the N5 methyl

114 CH3-H4folate) to the cob(I)amide center of a corrinoid/iron-sulfur protein (CFeSP), forming H4folate

115 ate (CH3-H4folate) to the cobalt center of a corrinoid/iron-sulfur protein (CFeSP), forming methylcob

116 The methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase (MeTr) f

117 The methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase (MeTr) f

118 The methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase (MeTr) f

119 -kDa (alpha2epsilonx) subunits and a 100-kDa corrinoid/iron-sulfur protein with the 60- and 58-kDa su

120 s, initially annotated as hypothetical fused corrinoid/methyl transfer proteins, which are highly ele

121 d protein, but no obvious means to carry out corrinoid methylation with glycine betaine.

122 ive site for dimethylamine demethylation and corrinoid methylation.

123 wledge, DSY3156 is the first glycine betaine:corrinoid methyltransferase described, and a designation

124 ould be accounted for by the activity of the corrinoid methyltransferase enzyme, suggesting fractiona

125 ere used to assay and purify a dimethylamine:corrinoid methyltransferase, MtbB1.

126 atures are the presence of a monomethylamine:corrinoid methyltransferase, the first time that this en

127 proteins related to MttB, the trimethylamine:corrinoid methyltransferase.

128 of the Mts system, a set of three homologous corrinoid/methyltransferase fusion protein isoforms invo

129 , mtsF and mtsH genes, which encode putative corrinoid/methyltransferase isozymes involved in methyls

130 In this review, we describe the corrinoid model for investigating microbial community in

131 dehalogenation with the respective purified corrinoids (norpseudovitamin B12 and norvitamin B12), as

132 processes across all domains of life require corrinoids, only a fraction of bacterial and archaeal sp

133 t interactions allowing for stabilization of corrinoids other than Cbl.

134 transferase that catalyze both ATP-dependent corrinoid phosphorylation and GTP-dependent guanylylatio

135 lamin in a chemically defined medium lacking corrinoid precursors.

136 corrinoids de novo, and instead must acquire corrinoids produced by other organisms in their environm

137 However, the profile of corrinoids produced in corrinoid-dependent microbial com

138 2 MAGs were assigned functional roles (e.g., corrinoid producers, acetate/H(2) producers, etc.).

139 -DFE was also demonstrated in vivo using the corrinoid-producing homoacetogen Sporomusa ovata, sugges

140 ssay data established adenosylcobyric as the corrinoid product of the CbiZ-catalyzed reaction.

141 s played by different phylogenetic groups in corrinoid production and corrinoid exchange within micro

142 imethylamine and specifically methylates the corrinoid prosthetic group of MtbC, which is subsequentl

143 sed of 51-kDa subunits that do not possess a corrinoid prosthetic group.

144 h three purified proteins: a monomethylamine corrinoid protein (MMCP), the "A" isozyme of methylcobam

145 und to a second polypeptide, monomethylamine corrinoid protein (MMCP).

146 The monomethylamine corrinoid protein and the A isozyme of methylcobamide:Co

147 These results show that MMCP is the major corrinoid protein for methanogenesis from monomethylamin

148 These results indicate that the 480-kDa corrinoid protein functions as a CoM methylase during me

149 These data indicate that the 480-kDa corrinoid protein is composed of a novel isozyme of meth

150 d in TMA-dependent methanogenesis, that this corrinoid protein is methylated by the substrate and dem

151 barkeri included the previously unidentified corrinoid protein MtbC, which copurified with MtbA, the

152 MtcB, along with the corrinoid protein MtqC and the methylcorrinoid:tetrahydr

153 encodes a bona fide methanol-utilizing MtaB/corrinoid protein pair.

154 This methyltransferase is the 480-kDa corrinoid protein previously identified by its methylati

155 Methylation of the corrinoid protein requires reduction of the central coba

156 MtpB along with MtqC (a corrinoid protein) and MtqA (a methylcorrinoid:tetrahydr

157 a methyl group originating from a methylated corrinoid protein, but no obvious means to carry out cor

158 he synthesis of acetyl-CoA from a methylated corrinoid protein, CO, and coenzyme A and (ii) the oxida

159 The genes encode a putative corrinoid protein, HgcA, and a 2[4Fe-4S] ferredoxin, Hgc

160 d to the Co(I) state for the monomethylamine corrinoid protein, MtmC.

161 polypeptide was therefore designated the TMA corrinoid protein, or TCP.

162 A 480-kDa corrinoid protein, previously demonstrated to be a CoM m

163 amine methyltransferase methylates a cognate corrinoid protein, which is subsequently demethylated by

164 lmethylether to the cobalt center of MtvC, a corrinoid protein.

165 t that RamA mediates reductive activation of corrinoid proteins and that it is the first functional a

166 ssesses the corrinoid binding motif found in corrinoid proteins involved in dimethylsulfide- and meth

167 nsferase) and MtsB (homologous to a class of corrinoid proteins involved in methanogenesis).

168 encoding putative methanol-specific MtaB and corrinoid proteins: mtaCB1, mtaCB2 and mtaCB3.

169 The corrinoid remained associated with the 26-kDa polypeptid

170 orrinoid requirements in a community through corrinoid remodelling, in this case, by importing extrac

171 oduced in all of the studied communities, in corrinoid remodelling.

172 (1.4%) and cobinamide (1.8%) (an incomplete corrinoid) represented a small portion of the total amou

173 hat D. mccartyi is capable of fulfilling its corrinoid requirements in a community through corrinoid

174 ring-contraction of the porphyrinoid to the corrinoid ring system.

175 ompleting biosynthesis from an intermediate (corrinoid salvaging).

176 from a molecule of ATP to a transient Co(1+)corrinoid species generated in the enzyme active site.

177 p from cosubstrate ATP to a transient Co (1+)corrinoid species generated in the enzyme active site.

178 promote the formation of the four-coordinate corrinoid species needed for activity.

179 computations reveals that this unique Co(2+)corrinoid species possesses an essentially square-planar

180 t between the Abs spectra of these two Co(2+)corrinoid species, MCD data reveal that substitution of

181 ive than that of the Co(2+/1+) couple of the corrinoid substrate.

182 r GTP than MjCobY(WT) but failed to bind the corrinoid substrate.

183 ropriate mimic of the physiological base-off corrinoid substrate.

184 amically challenging reduction of its Co (2+)corrinoid substrates, we have examined how the enzyme ac

185 ids aerobically by importing an "incomplete" corrinoid, such as cobinamide (Cbi), and adding appropri

186 and production of intermediates required for corrinoid synthesis) displayed significant coexpression

187 C (a corrinoid-binding protein), and MtqA (a corrinoid:tetrahydrofolate methyltransferase).

188 ection method to examine relationships among corrinoids, their lower ligand bases and specific microb

189 ATP-dependent reductive activation of Co(II) corrinoid to the Co(I) state for the monomethylamine cor

190 also provide evidence for the regulation of corrinoid transport and biosynthesis.

191 is the first genetic analysis of an archaeal corrinoid transport system.

192 The total number of distinct corrinoid transporter families in the human gut microbio

193 We show that BtuB, the outer membrane corrinoid transporter, mediates the uptake of all three

194 epsilonCl of PCE depended in addition on the corrinoid type: epsilonC/epsilonCl values of 4.6 and 5.0

195 that was preincubated with ATP, both Co (2+)corrinoids undergo a partial ( approximately 40-50%) con

196 inoids interact only weakly with CobA, Co(2+)corrinoids undergo partial conversion to a new paramagne

197 existence of a putative ABC transporter for corrinoid utilization in the extremely halophilic archae

198 Corrinoid (vitamin B12-like) cofactors contain various a

199 PCE dehalogenation by enzymes and different corrinoids, whereas such differences were not observed f

200 ide and subsequently demethylates MtsB-bound corrinoid with coenzyme M, possibly employing elements o