ライフサイエンスコーパス: cosubstrate

1 using 2-propanol (30%, v/v) as cosolvent and cosubstrate.

2 Both enzymes require dioxygen as a cosubstrate.

3 me by addition of the substrate prior to the cosubstrate.

4 resence of recombinant G9a enzyme and AdoMet cosubstrate.

5 cleaver identified, is used as the reducing cosubstrate.

6 or Ca(2+) in its second role as a nucleotide cosubstrate.

7 that LPMOs can use H2O2, instead of O2, as a cosubstrate.

8 DP donor to the beta-OHTyr-6 of the aglycone cosubstrate.

9 ar substrates with TcdB yet uses a different cosubstrate.

10 ge that reduces ALKBH7's ability to bind its cosubstrate.

11 te substitute poorly for 2-oxoglutarate as a cosubstrate.

12 rved rate-limiting step involving release of cosubstrate.

13 y a Pictet-Spengler reaction with a carbonyl cosubstrate.

14 accommodates the adenosyl moiety of the NAD+ cosubstrate.

15 vated by a human enzyme in the presence of a cosubstrate.

16 dependent on the concentration of the oxygen cosubstrate.

17 ltetrahydropterin, respectively, was used as cosubstrate.

18 corresponding sulfinic acids using O(2) as a cosubstrate.

19 mption of the metabolically valuable alphaKG cosubstrate.

20 O chitin-binding protein CBP21 using H2O2 as cosubstrate.

21 PylB catalysis rather than canonically as a cosubstrate.

22 lize either oxygen or hydrogen peroxide as a cosubstrate.

23 lize either oxygen or hydrogen peroxide as a cosubstrate.

24 xidase utilizing NO as an electron donor and cosubstrate.

25 erized to date require molecular oxygen as a cosubstrate.

26 oxidoreductase (EmoB) to provide FMNH2 as a cosubstrate.

27 duces dehydroalanine to D-Ala using NAPDH as cosubstrate.

28 erminal alkenes using hydrogen peroxide as a cosubstrate.

29 otinamide adenine dinucleotide (NAD(+)) as a cosubstrate.

30 ced holomycin to holomycin utilizing O(2) as cosubstrate.

31 o depend upon the concentration of the other cosubstrate.

32 ncentrations of S-alkylglutathiones serve as cosubstrates.

33 between the reductant and oxygen-containing cosubstrates.

34 ate, and cytidine triphosphate were suitable cosubstrates.

35 ch as l-cysteine and beta-mercaptoethanol as cosubstrates.

36 ormula: see text] and [Formula: see text] as cosubstrates.

37 g) and then at P(gamma) (ADP-forming) of ATP cosubstrates.

38 avin reductase), chloride ion, and oxygen as cosubstrates.

39 n utilizing oxygen or hydrogen peroxide as a cosubstrate?

40 ared to Km(1a) 3.4 +/- 0.4 mM when 2a is the cosubstrate].

41 finity for the substrate GSH but not for the cosubstrate 1-chloro-2,4-dinitrobenzene (CDNB).

42 rand jellyroll core with both Fe(II) and the cosubstrate 2-oxoglutarate bound in the active site.

43 t requires the dioxygenase cofactor iron and cosubstrate 2-oxoglutarate.

44 N-oxalylglycine, which is an analogue of the cosubstrate 2-oxoglutarate.

45 rather than utilizing the typical keto-acid cosubstrates, 2-oxoglutarate, pyruvate, and oxaloacetate

46 efficient versus the original dipolarophile cosubstrate (2m and 2a, respectively), reveals that whil

47 ture of Teg12, Teg12 bound to the desulfated cosubstrate 3'-phosphoadenosine 5'-phosphate, and Teg12

48 18-kDa PCP fragment and by radioassay using cosubstrate [3H] pantetheinyl-coenzyme A for all PCP-con

49 ((carboxylmethyl)amino)butanoic acid (CABA), cosubstrate a-ketoglutarate, and an Fe(IV)=O mimic oxova

50 In addition to substrate and cosubstrate, ACCO requires the activator CO(2) for conti

51 tyltransferase catalytic domain bound to its cosubstrate acetyl-CoA at 1.05 A resolution.

52 Excitingly, the ternary complex of HygX with cosubstrate alpha-ketoglutarate and putative product hyg

53 e results, in Fe(II)-reconstituted TfdA, the cosubstrate alpha-KG chelates to the metal via a C-1 car

54 coupled to the oxidative decarboxylation of cosubstrate, alpha-ketoglutarate (alpha-KG).

55 e conducted on TauD samples treated with NO, cosubstrate alphaKG, and either protonated or specifical

56 heir substrates and decarboxylation of their cosubstrate, alphaKG, to CO(2) and succinate.

57 11.6 muM for dimethylallyl diphosphate as a cosubstrate and 24.3 muM for GDP.

58 complex with the 10-formyl-5,8-dideazafolate cosubstrate and a glycinamide ribonucleotide analogue, h

59 also known as 2-oxoglutarate) as an obligate cosubstrate and are inhibited by succinate, fumarate, an

60 also known as 2-oxoglutarate) as an obligate cosubstrate and are inhibited by succinate, fumarate, an

61 The role of natural DOM as potential cosubstrate and detoxification reactant may improve futu

62 An absolute requirement for dioxygen as a cosubstrate and iron as cofactor suggests that HIF-PH fu

63 otinic acid adenine dinucleotide (NAAD) as a cosubstrate and is inhibited by nicotinic acid.

64 irrespective of which aminoglycoside is the cosubstrate and occurs without a significant change in t

65 s as well as compounds that bind across both cosubstrate and primary substrate binding sites.

66 ration of the Fe(II) in the presence of both cosubstrate and substrate appears to be critical for the

67 The enzyme requires molecular oxygen as a cosubstrate and utilizes a noncovalently bound flavin co

68 Kinetic parameters of peptide and NAD(+) cosubstrates and IC(50) values of standard reference inh

69 vity in the presence of a small DNA oligomer cosubstrate, and chi-cutting activity.

70 P4H uses alpha-ketoglutarate and O2 as cosubstrates, and forms succinate and CO2 as well as Hyp

71 They use only NADPH and oxygen as cosubstrates, and produce only NADP(+) and water as bypr

72 ant forms the five-coordinate site when both cosubstrates are bound, the Fe(II) site of the Glu280Lys

73 lii lethal toxin (TcsL), which uses the same cosubstrate as TcdB but shares Rac only as a common subs

74 PvdA uses the same cosubstrates as several flavin-dependent hydroxylases th

75 te site only when both the substrate ACC and cosubstrate ascorbate are bound to the enzyme.

76 ous enzyme that couples the oxidation of the cosubstrate ascorbate to the oxidation of substrate ACC

77 12) by transferring the adenosyl group from cosubstrate ATP to a transient Co (1+)corrinoid species

78 In the presence of cosubstrate ATP, ACATs raise the Co(II)/Co(I) reduction

79 The cosubstrate, ATP, enforces a four-coordinate cob(II)alam

80 strates indirectly via the regulation of ADO cosubstrate availability.

81 presence of cobalt and vice versa, with the cosubstrates being transported in a 1:1 ratio.

82 res and had features consistent with Cbl and cosubstrate binding capabilities.

83 tural differences that reflect substrate and cosubstrate binding effects and implies roles for the in

84 ancer-associated mutation affects productive cosubstrate binding in ALKBH7.

85 antly decreases V(max) with little effect on cosubstrate binding in G117C, G117S, and G117N mutants.

86 mutant exhibited little difference in either cosubstrate binding or stimulation of melibiose transpor

87 elical 'lid' region that gates access to the cosubstrate binding pocket and an active center that con

88 sferase displays a relatively well-conserved cosubstrate binding pocket but is unique in its active s

89 red in the apo form but becomes ordered upon cosubstrate binding.

90 ly conserved Thr-467, which is implicated in cosubstrate binding.

91 e, resulting in the reorientation of several cosubstrate-binding residues.

92 Both cosubstrate-binding sites are mainly contributed by the

93 Thus it seems that oxygen (the cosubstrate) binds in a different site to that observed

94 ET efficiency compared to the enzyme with no cosubstrate bound.

95 Thus NADPH is not only crucial as a cosubstrate, but can also act as a molecular chaperone t

96 3-MeBFPP, is a substrate with three peptide cosubstrates, but is not an effective substrate with a f

97 as substrates for the alkylation of peptide cosubstrates by yeast protein farnesyl transferase.

98 TS that does not require CH(2)H(4)folate as cosubstrate, by mutant TSs was analyzed and showed that

99 gards to kcat and Km values for dUMP and the cosubstrate CH2H4-folate.

100 ored by 1.7-10.0-fold in the presence of the cosubstrate CoASH, while the entropy becomes 2.0-22.5-fo

101 Cosubstrate coenzyme A (CoA), the phosphopantetheinyl gr

102 This emergent behavior, which we named cosubstrate compensation (CSC), enables the ETC to maint

103 trates under conditions with a subsaturating cosubstrate concentration and uncompetitive against preQ

104 LacY cause effects on sugar recognition and cosubstrate coupling that are stereotypical of neutral s

105 ffects on melibiose transport and binding of cosubstrates depended on the physical-chemical propertie

106 RoxA incorporates both oxygen atoms of its cosubstrate dioxygen into the rubber cleavage product OD

107 d holo forms of AKR11A demonstrates that the cosubstrate does not play the dramatic role in active si

108 The addition of reducing cosubstrate during reaction of PGHS-1 with peroxide prot

109 2 ideal for studying redox-state cofactor or cosubstrate dynamics.

110 e reactivities of nonnative organic peroxide cosubstrates effectively exclude the possibility of a pi

111 his corrects a longstanding misperception of cosubstrate effects, provides more realistic kinetic con

112 In the absence of cosubstrate, enzyme catalyzed the enolization/proton exc

113 reducing equivalents for its healthy growth; cosubstrate experiments support this finding.

114 Its cosubstrate, FADH(2), is supplied by HpaC, an NAD(P)H-fl

115 Microsomal uptake of the cosubstrate for all glucuronidation reactions, UDP-glucu

116 mes responsible for producing the obligatory cosubstrate for all sulfotransferases.

117 NADPH is an obligatory cosubstrate for iNOS synthesis of NO.

118 ,200-Da homodimer, and FMNH2 is an essential cosubstrate for its activity.

119 S-adenosyl-l-methionine (SAM) is a necessary cosubstrate for numerous essential enzymatic reactions i

120 trons from one reaction to another, and as a cosubstrate for other enzymes such as the sirtuins and p

121 sphate-modified ATP analog, ATP-biotin, as a cosubstrate for phosphorylbiotinylation of NME1 and NME2

122 thanol (FcMeOH/FcMeOH(+)), which acts as the cosubstrate for the enzymatic process.

123 ld be expected if Ca(2+) were only used as a cosubstrate for the nucleotide substrate, but rather fol

124 Hydrogen peroxide is a cosubstrate for the oxidative cleavage of saccharidic su

125 3'-phosphoadenosine-5 '-phosphosulfate, the cosubstrate for the sulfonation reaction.

126 Many cosubstrates for the peroxidase activity of prostaglandi

127 dinitrosyliron complex (DNIC) and preventing cosubstrates from binding.

128 g enzyme (k(cat) = 250 s(-)1) that binds the cosubstrate fructose 6-phosphate (Fru-6-P) with relative

129 es not alter the apparent affinities for the cosubstrates glutamate or sodium.

130 affinity (KD =130 nm) in the presence of the cosubstrate glutathione (GSH).

131 m transfer and halogenation reactions (using cosubstrates guaiacol, thioanisole, and monochlorodimedo

132 the normal order of addition of the oxidant cosubstrate (H(2)O(2)) before organic substrate [2,4,6-t

133 peroxidase order of addition of the oxidant cosubstrate (H(2)O(2)) followed by organic substrate (TC

134 nucleotide (NAD(+)) is an enzyme cofactor or cosubstrate in many essential biological pathways.

135 chain that blocks the site for a nucleophile cosubstrate in PRTases.

136 , 13-diacetylbaccatin III and benzoyl-CoA as cosubstrates in the corresponding cell-free extract.

137 evel activity is observed with H(2)O(2) as a cosubstrate indicating an important role in ET for O(2)

138 ormation about the substrate specificity and cosubstrate inhibition of the enzyme.

139 We present evidence that an organic cosubstrate inhibits NDFO, likely by keeping periplasmic

140 pport the view that translocation of the UGT cosubstrate is a rate-limiting step of the glucuronidati

141 he less reactive LThDP intermediate when its cosubstrate is absent.

142 We show here that the preferred cosubstrate is actually H2O2 and that HppE therefore alm

143 strongly on whether the succinyl CoA (SCoA) cosubstrate is bound at the MmOGOR active site.

144 d in conditions under which the substrate or cosubstrate is consumed in a multielectron redox process

145 with NADPH, in which the redox state of the cosubstrate is directly validated by single-crystal spec

146 The loop that folds over the NAD(P)H cosubstrate is disordered in the apo form but becomes or

147 oxidatic activity with different peroxidatic cosubstrates is comparable for KatA and KatB, but KatA i

148 In previous studies, using ATP as the cosubstrate, it was reported that the APH(2'')-Ia domain

149 tude of that observed with its natural thiol cosubstrate, L-homocysteine.

150 PEP appears to depend on the presence of the cosubstrate MgATP.

151 ctor, are used to deliver electrons from the cosubstrate NADPH to molecular oxygen, analogous to othe

152 l reductase domain binds FAD and FMN and the cosubstrate NADPH.

153 method, the kinetics of the reaction of the cosubstrate nicotinamide adenine dinucleotide and the co

154 H(2)O(2) inhibition by converting it to the cosubstrate O(2).

155 roxide (H2O2) and bromide (Br-), a preferred cosubstrate of EPO, to generate the cytotoxic oxidant hy

156 t conjugates to a bovine serum albumin (BSA) cosubstrate of larger hydrodynamic mass.

157 duction of alpha-ketoglutarate (alpha-KG), a cosubstrate of TATase required to regenerate the pyridox

158 of GOx was not oxidized by O(2), the natural cosubstrate of the enzyme.

159 ated that GTP, and not ATP, is the preferred cosubstrate of this enzyme.

160 Because key metabolites are cofactors or cosubstrates of chromatin-modifying enzymes, changes in

161 ty can seriously overestimate the effects of cosubstrate on cyclooxygenase velocity.

162 e effect of the concentration of the various cosubstrates on the POD reaction rate demonstrated that

163 llographic analyses reveal inhibition by 2OG cosubstrate or primary substrate competitors as well as

164 zyme to the mixed-valent form, and O(2), the cosubstrate, oxidizes the fully reduced form to the mixe

165 the addition of substrate, TBP, prior to the cosubstrate, peroxide.

166 pted re-examination of the effect of several cosubstrates (phenol, acetaminophen, N,N,N',N'-tetrameth

167 tion consists of the formation of a cofactor(cosubstrate)-PKMT-substrate complex, lysine deprotonatio

168 Addition of SAM tightened up the cosubstrate pocket in both proteins and prevented the al

169 hibitors spanning both the substrate and the cosubstrate pockets.

170 e measurements with a chromogenic peroxidase cosubstrate previously were used to evaluate the kinetic

171 The cosubstrates produced only a slight (10-60%) stimulation

172 We recently demonstrated the substrate and cosubstrate promiscuity of the wild-type human ChAT (hCh

173 milarly, targeting TET1 activity by removing cosubstrate promoted apoptosis and DNA damage.

174 (NO) interactions as they may relate to the cosubstrate, pseudo halide thiocyanate (SCN(-)), and sub

175 nding mode spanning the binding sites of the cosubstrate pterin and the substrate tryptophan and by c

176 ant for catalysis, specifically those of the cosubstrate pterin, the substrate tryptophan as well as

177 e presence of O2, chloride ion, and L-Trp as cosubstrates, purified RebH displays robust regiospecifi

178 conventional conformation of the bound NADP+ cosubstrate, raising the possibility that it donates the

179 on than the WT enzyme in the presence of the cosubstrate/reductant ascorbate and that this process is

180 flavoprotein monooxygenases and their common cosubstrate requirements, the reaction of FADH(2) and O(

181 mall molecule PRMT5 inhibitors engage either cosubstrate S-adenosyl methionine (SAM) or peptide-subst

182 s as a binary complex with its desmethylated cosubstrate S-adenosyl-l-homocysteine.

183 The cosubstrate S-adenosylmethionine (SAM) stabilizes the ac

184 productive CO(2) loss in the presence of the cosubstrate S-adenosylmethionine (SAM), with S-adenosylh

185 Addition of the cosubstrate, S-adenosylmethionine (SAM), prevents degrad

186 controls reactivity by interactions with the cosubstrate, SAM, which is bound to the catalytic iron-s

187 (-) or Cl(-) is oxidized in the absence of a cosubstrate, show that only modest tuning is required to

188 l Cbl analogues using glutathione or NADH as cosubstrates, similarly to previously characterized CblC

189 domain; however, glycerol is not acting as a cosubstrate since no glycerophosphate product was detect

190 , with a completely unoccupied substrate and cosubstrate site, while the ternary complex is the first

191 by simultaneously blocking the substrate and cosubstrate sites, as revealed by structural analyses.

192 main that is bound at both the substrate and cosubstrate sites.

193 n between MTS derivatives and A395C, and the cosubstrate, sodium, slows reaction of MTSEA with Y405C

194 Using lipid II precursor as cosubstrate, sortase A catalyzes the amide linkage betwe

195 ere previously shown to dictate the acyl-CoA cosubstrate specificity in the carnitine series.

196 Similar alterations in nucleophile cosubstrate specificity were also observed.

197 Screening of potential 2OG cosubstrate substitutes reveals that certain 2-oxoacids,

198 ently distinct molecular mechanisms coupling cosubstrate/ substrate binding to catalytic activity.

199 nt on the concentrations of their respective cosubstrates, suggesting that both ligands bind randomly

200 and dioxygen for catalysis with the alpha-KG cosubstrate supplying the additional reducing equivalent

201 might regenerate 2-oxoglutarate, which is a cosubstrate that facilitates the breakdown of alanine by

202 NAD(+)) is an endogenous enzyme cofactor and cosubstrate that has effects on diverse cellular and phy

203 PAPS is the obligate cosubstrate that is synthesized in tissues to make avail

204 te conformational equilibrium in the alphaKG cosubstrate that modulates the O(2) accessibility of the

205 Furthermore, when 2m is the cosubstrate, the affinity of 29G12 for its dipole 1a is

206 Besides the H(2)O(2) cosubstrate, the cleavage of glycosidic bonds by LPMOs d

207 yrrolyl-2-carboxyl-thioester of CouN1 is the cosubstrate, the in vitro product differs from clorobioc

208 ibition using dihydrofolate and NADPH as the cosubstrates, the previously unreported compounds 2 and

209 Peroxidase cosubstrates thus have a much smaller stimulatory effect

210 fectively uses plasma levels of bromide as a cosubstrate to brominate bases in nucleotides and double

211 nergy from the downhill translocation of one cosubstrate to catalyze the accumulation of the other.

212 r non-heme-iron(II) cofactor and dioxygen as cosubstrate to cleave these C-H bonds and direct the rin

213 , the AcuC protein did not require NAD(+) as cosubstrate to deacetylate AcsA(Ac).

214 ses iron as its cofactor and dioxygen as its cosubstrate to effect the unique, ring-cleaving, four-el

215 H2O2 by utilizing plasma levels of Br- as a cosubstrate to form potent brominating agents.

216 strates while decomposing the 2-oxoglutarate cosubstrate to form succinate and CO(2).

217 r hydrogen peroxide or molecular oxygen as a cosubstrate to generate a reactive halogen atom for cata

218 e, and the ferrocenium methanol acted as the cosubstrate to GOx to catalyze the oxidation of glucose

219 tion catalyst, using coenzyme A (CoASH) as a cosubstrate to phosphopantetheinylate Ser880 of the 155-

220 have generally employed dithiothreitol as a cosubstrate to reductively cleave the persulfide interme

221 four electrons per turnover to reduce the O2 cosubstrate to the oxidation state of water and incorpor

222 ation number change with the binding of both cosubstrates to the enzyme, and determines this to be du

223 eficient in binding a particular cofactor or cosubstrate, to complement each other by generating NO u

224 In complex with the cosubstrate UDP-N-acetylglucosamine (UDP-GlcNAc),O-linke

225 The IPP analogue (3-ClIPP) was a cosubstrate when incubated with dimethylallyl diphosphat

226 enzymes is their utilization of NAD(+) as a cosubstrate, which has led to the suggestion that Sir2 a

227 In the absence of added reducing cosubstrates, which maximizes the levels of oxidized enz

228 rationale, catalysis by CD-0387 with 2-ME as cosubstrate, while less efficient, is not subject to pot

229 (CT)) have been successfully employed as the cosubstrate with S. lentus in the biodegradation of an a

230 12 accepts a bulky hydrophobic dipolarophile cosubstrate, with rates of product formation up to 70-fo

231 three enzymes efficiently used H(2)O(2) as a cosubstrate, yielding product profiles identical to thos