ライフサイエンスコーパス: cps

1 cps of serotypes 6A/6B have wciNalpha, encoding alpha-1,

2 cps-6 encodes a homologue of human mitochondrial endonuc

3 A baseline viral load >=100'000 cps/mL (multivariable Hazard Ratio (mHR): 2.2, 95% CI: 1

4 and 250- to 750-keV energy windows was 30.03 cps/kBq (3.3%) and 49.11 cps/kBq (5.4%), respectively.

5 The peak sensitivity reached 1.3% (13,080 cps/MBq).

6 zed to KD (0.3 g/kg vegetable proteins and 1 cps/5 kg ketoanalogues per day) or continue LPD (0.6 g/k

7 luded patients treated with Colicron((R)) (1 cps/day for 8 weeks).

8 utations (DRMs), 46 (43.4%) with VL >/= 1000 cps/mL and >/=1 DRMs; no HIV RNA data was available for

9 VL < 1000 cps/mL, 7 (6.6%) with VL >/= 1000 cps/mL and no drug resistance mutations (DRMs), 46 (43.4

10 Among participants with VLs >/=1000 cps/mL, 22 of 32 (69%) harbored drug resistance mutation

11 If VL was >/=1000 cps/mL, genotyping was performed.

12 cted in 22% of participants with VLs >/=1000 cps/mL.

13 ctively performed if viral load (VL) >/=1000 cps/mL.

14 , of whom 18 (17.0%) switched with VL < 1000 cps/mL, 7 (6.6%) with VL >/= 1000 cps/mL and no drug res

15 y windows was 30.03 cps/kBq (3.3%) and 49.11 cps/kBq (5.4%), respectively.

16 three isogenic pneumococcal strains with 11A cps loci containing wcrL encoding Ser-112 (MBO128) or Al

17 he in vivo node counts ranged from 0 to 1228 cps, while ex vivo counts ranged from 0 to 1516 cps.

18 (-4) cps per detector (corresponding to 0.13 cps over the whole array).

19 idence time resolution and sensitivity of 14 cps/kBq.

20 , while ex vivo counts ranged from 0 to 1516 cps.

21 rcially available ultra-high-resolution (171 cps/kBq) total-body digital PET/CT device with a 194-cm

22 MBq for mouse collimators and from 53 to 175 cps/MBq for rat collimators.

23 ensitivity was 83 cps/kBq for MRD 85 and 176 cps/kBq for MRD 322.

24 ensitivities (180-500 cps/ppb versus 1.6-3.2 cps/ppb) for F detection.

25 The average sensitivity was 5.2 cps/kBq.

26 rected cdb3-specific SPR signal was 98 +/- 2 cps microCi-1 [mumol band 3]-1.

27 cond per square millimetre (cps mm(-2)) to 2 cps mm(-2) at room temperature, enabling much better res

28 participants on ART with HIV-1 RNA below 20 cps/ml.

29 howcases the high sensitivity of 0.13%~0.21% cps/Bq.

30 ture with 1.5-mm spatial resolution and 13.3 cps/microCi (0.359 cps/kBq) sensitivity.

31 D and 3D was measured as 1.7 cps/kBq and 7.3 cps/kBq, respectively.

32 over 10 seconds] ranged from 3346 to 47,300 cps and was highly dose-dependent (r = 0.90, P = 0.0002)

33 atial resolution and 13.3 cps/microCi (0.359 cps/kBq) sensitivity.

34 -acetyltransferase genes in the serotype 35C cps locus suggested that it could be incomplete, as the

35 In contrast, the HS:23, HS:36 and HS:23/36 cps sequences were highly conserved.

36 asured with a 70-cm-long line source is 4.36 cps/kBq, whereas the scatter fraction is 40% with a 20 x

37 phylogenetically distant serogroup 9 and 36 cps loci, which all encode this distinguishing glycosidi

38 s) and had a dark-count rate of 1.0 x 10(-4) cps per detector (corresponding to 0.13 cps over the who

39 pants, 14.6%, 15.2% and 11.1% had VLs >/=400 cps/mL at 12, 24, and 36 months, respectively.

40 r having a detectable viral load (VL, >/=400 cps/mL) using Cox models.

41 The system sensitivity varies from 29 to 404 cps/MBq for mouse collimators and from 53 to 175 cps/MBq

42 lts from the commercial probe (8.75 +/- 0.47 cps/kBq).

43 by v2.0 with an HIV-1 RNA mean value of 3.5 cps/ml.

44 g a high Cs sensitivity of more than 1.10(5) cps/(ng g(-1)) and low background levels at m/z 135 and

45 of magnitude improved sensitivities (180-500 cps/ppb versus 1.6-3.2 cps/ppb) for F detection.

46 und levels at m/z 135 and 137 lower than 0.6 cps.

47 easured with a 70-cm-long line source is 6.6 cps/kBq, whereas scatter fraction is 27% measured with a

48 sensitivity in 2D and 3D was measured as 1.7 cps/kBq and 7.3 cps/kBq, respectively.

49 NU2-1994 analysis, the sensitivity was 12.7 cps/Bq/mL (444 kcps/microCi/mL), the scatter fraction wa

50 The averaged sensitivity was 13.7 cps/kBq at the center of the field of view.

51 R-TOF 10k achieves sensitivities up to 80000 cps ppbV(-1) and detection limits down to 0.5 pptV for a

52 The sensitivity was 83 cps/kBq for MRD 85 and 176 cps/kBq for MRD 322.

53 evels of MDOs act to signal RcsC to activate cps expression is proposed.

54 igh sequence homology between the cps-6B and cps-6A loci, serotypes 6A and 6B cannot be differentiate

55 observations demonstrated that cps(Ia)H and cps(III)H encoded the type Ia and III CPS polymerases, r

56 occurring in only two nonclade isolates and cps in four.

57 ronmental isolates revealed that the prp and cps amplicons were detected only in clinical isolates id

58 Oligonucleotide primers targeting prp and cps were combined in a multiplex PCR method that defines

59 ion did not increase drought resistance, and cps mutants in other cultivars did not alter susceptibil

60 RcsF in the activation of both the rprA and cps promoters.

61 In this study we sequenced biosynthetic cps regions, ranging in size from 15 to 34 kb, from sele

62 o high-level constitutive expression of both cps and rprA suggests that the response regulator domain

63 nd low Mg2+, resulting in expression of both cps and ugd genes.

64 Tumor-associated CD11c(+) cells invaded by cps were converted to immunostimulatory phenotypes, whic

65 This is the largest known Campylobacter cps cluster (38 kb excluding flanking kps regions), whic

66 Capsule (cps) gene expression in Escherichia coli is controlled b

67 were characterized for one of nine capsule (cps) genotypes.

68 The capsule (cps) locus of Streptococcus pneumoniae is flanked by the

69 In the capsule gene cluster (cps) of Nm, region A contains the genetic information fo

70 ete sequence of the respective gene cluster (cps).

71 equence motif, conserved between the E. coli cps promoter and the Erwinia amylovora ams promoter and

72 Lastly, common cps gene (cps2ABCD) mutants did not show significant abn

73 by deletion of wcrD from each corresponding cps locus.

74 ed with specific genes in each corresponding cps locus.

75 Correspondingly, cps treatment markedly increased tumor antigen-specific

76 liquid, decrease biofilm formation, decrease cps gene expression, and suppress the DeltascrABC phenot

77 nsive biofilms and aggregates than a defined cps mutant, suggesting that additional factor(s) contrib

78 cross-reactive IgG in sera from TIGR4 Delta cps-colonized mice, with a modest contribution from PpmA

79 Comparison of the determined cps sequences of the HS:1, HS:19 and HS:41 strains with

80 ants with cpsS defects have greatly elevated cps transcription; their high level of cpsA expression w

81 a 35.5-kb DNA fragment containing the entire cps operon.

82 ver, unlike VpsR, CpsR was not essential for cps expression.

83 ovel genomic organizations within functional cps loci, consisting of insertion sequence (IS) elements

84 Capsule gene (cps) expression, which normally occurs at low levels in

85 d polysaccharide capsule biosynthetic genes (cps genes) are primarily clustered at one site located a

86 ivator of the capsular polysaccharide genes (cps), has been identified in Escherichia coli.

87 of capsular polysaccharide synthesis genes (cps) and restriction enzyme digestion.

88 The genes (cps) involved in the synthesis of the colanic acid capsu

89 equencing of nine additional adjacent genes, cps(III)FGHIJKL, neu(III)B, and neu(III)C.

90 f an attenuated strain of Toxoplasma gondii (cps) that cannot replicate in vivo and therefore is not

91 th distinct serologic properties, homologous cps loci, and structurally similar PSs.

92 lar to those of some of the other identified cps loci.

93 The present study identifies cps genes and examines their regulation.

94 ants did not show significant abnormality in cps transcription, although they produced significantly

95 Single amino acid changes in cps genes encoding glycosyltransferases can alter substr

96 "up" mutation (i.e., leading to increase in cps transcription) that normally results in constitutive

97 litates the detection of DNA polymorphism in cps genes and correlates well with serotyping.

98 e between the operons was found to reside in cps(III)H, the putative CPS polymerase gene.

99 ns FWHM in mm (point source sensitivities in cps-MBq) for half-cone beam, fan-beam and parallel-beam

100 e with phase variations or internal stops in cps loci, which warrant further characterization by addi

101 pathway; CpsR was required for the increased cps expression observed in scrA deltaopaR strains.

102 ular pneumococcus virulent, and interspecies cps transfer should be considered a potential mechanism

103 Indeed, intraperitoneal cps treatment triggered rejection of established ID8-Veg

104 e acquired a 24 kb capsule synthesis island (cps) by horizontal gene transfer which consists of a syn

105 d in nature, if pneumococcus can replace its cps with oral streptococcal cps, it may increase its ser

106 vasive E. coli isolates, we show that the K1-cps locus is present in a quarter of bloodstream infecti

107 Strikingly, all of the cv Kitaake cps mutants exhibit significantly increased susceptibili

108 The molecular interplay of additional known cps regulators was defined by establishing that CpsS, an

109 eumococcal strains carried pneumococcus-like cps loci (approximate size, 18-25 kb) that showed >70% n

110 The treatment required live cps that could invade cells and also required CD8(+) T c

111 capsular polysaccharide (PS) synthesis loci (cps).

112 e 1 (86%) with variations in capsular locus (cps) and prophage content.

113 eloped to interrogate the capsulation locus (cps) of vaccine serotypes to locate primer pairs in cons

114 , and (v) the capsular polysaccharide locus (cps) did not amplify during the initial MP-PCR but was p

115 Previous work on the meningococcal cps complex in Escherichia coli K-12 indicated that dele

116 000 counts per second per square millimetre (cps mm(-2)) to 2 cps mm(-2) at room temperature, enablin

117 Among the non-cps genes identified as conditionally essential was relA

118 /RcsB-mediated transcriptional activation of cps genes.

119 to an inhibitor of swarming and activator of cps expression.

120 In silico analysis of cps from 92 serotypes indicated that a primer pair spann

121 Immunochemotherapeutic applications of cps might be broadly useful to reawaken natural immunity

122 The therapeutic benefit of cps treatment relied on expression of IL-12, but it was

123 Identifying functional determinants of cps-encoded enzymes and their impact on capsule PS struc

124 Expression of cps(III)H in a type Ia strain resulted in suppression of

125 were associated with increased expression of cps-encoded proteins.

126 ne betaine enhanced the osmotic induction of cps::lacZ by both sucrose and NaCl but had no effect alo

127 Direct intratumoral injection of cps has efficacy against an inducible genetic melanoma m

128 Loss of cps-6 delays breakdown of mitochondrial inner membranes,

129 Reduction of cps-6 activity caused by a genetic mutation or RNA-media

130 In conclusion, although having no impact on cps transcription or the synthesis of the basal repeatin

131 putative E. faecium capsular polysaccharide (cps) biosynthetic locus, with different mutations arisin

132 ranscription of the capsular polysaccharide (cps) locus is not well understood.

133 of the biosynthetic capsular polysaccharide (cps) locus.

134 were members of the capsular polysaccharide (cps) operon.

135 lence determinants: capsular polysaccharide (cps), pneumolysin (ply), and pneumococcal surface protei

136 tentially conferring O-antigenic properties (cps and flp).

137 of diverse lytic viruses targeting relevant cps variants and allowing for variable prophage content.

138 d serotype 42 was unclear, as their reported cps loci are nearly identical.

139 ay versions, but average values of HIV-1 RNA cps/ml were 2.7-fold higher for v2.0 than v1.0.

140 c Nicotiana benthamiana plants expressing Rs-cps dsRNA were obtained and studied.

141 The transcript abundance of Rs-cps dsRNA appeared to be diverse in the different transg

142 R. similis and the transcription level of Rs-cps in R. similis was drastically decreased.

143 ment from the protease gene, cathepsin S (Rs-cps), was cloned into the binary vector pFGC5941 in the

144 Our results suggest that Rs-cps is essential for the reproduction and pathogenicity

145 eover, the bioassay results revealed that Rs-cps transgenic N. benthamiana plants were resistant to R

146 loy in planta RNAi approach to target the Rs-cps gene for the control of plant parasitic nematodes.

147 hing rate of R. similis isolated from the Rs-cps transgenic plants were also significantly reduced.

148 at a rate of 1.1 x 10(5) counts per second (cps) and had a dark-count rate of 1.0 x 10(-4) cps per d

149 the tumor injection site [counts per second (cps) averaged over 10 seconds] ranged from 3346 to 47,30

150 long line source is 4.79 counts per second (cps)/kBq.

151 gamma mode (5.59 +/- 0.41 counts per second [cps]/kBq) compared surprisingly well with the results fr

152 We also show that transferring SK95 cps into noncapsulated, avirulent pneumococcus gave it t

153 sular pneumococcus was transformed with SK95 cps, the transformant became virulent and killed all mic

154 g avirulent uracil auxotroph vaccine strain (cps) of Toxoplasma triggers novel innate immune response

155 can replace its cps with oral streptococcal cps, it may increase its serotype repertoire.

156 tivated genes, such as the capsule synthesis cps operon, requires the co-activator protein RcsA, wher

157 csA-dependent colanic acid capsule synthesis cps operon.

158 analysis of pneumococcal capsule synthesis (cps) loci suggested the existence of capsule subtypes am

159 ranscription of genes for capsule synthesis (cps) requires both RcsA and RcsB; transcription of other

160 on of the capsular polysaccharide synthesis (cps) loci of the 2 subtypes identified disruption of the

161 terize the capsule polysaccharide synthesis (cps) locus, classify N. meningitidis serogroups, and ide

162 es in the capsular polysaccharide synthesis (cps) locus.

163 on of the capsular polysaccharide synthesis (cps) operon responsible for CA biosynthesis in commensal

164 etic variation of the capsular PS synthesis (cps) locus is the molecular basis for structural and ant

165 capsules, our observations demonstrated that cps(Ia)H and cps(III)H encoded the type Ia and III CPS p

166 Taken together, our results establish that cps preferentially invades tumor-associated antigen-pres

167 The cps loci of 11A and 11D differ by one codon (N112S) in w

168 The cps monotherapy rapidly modified the tumor microenvironm

169 The cps treatment stimulated a strong CD8(+) T cell-mediated

170 encoding adenylsuccinate synthetase, and the cps operon required for capsule production.

171 es, are in a chromosomal region known as the cps locus.

172 would then involve PBP genes, as well as the cps operon, and would change both the serotype and the r

173 ue to the high sequence homology between the cps-6B and cps-6A loci, serotypes 6A and 6B cannot be di

174 Both the cps and ply/pspA mutants of a virulent type 6A isolate w

175 We identified mutations in the cps capsule gene cluster, previously unidentified transc

176 Transposon insertions in the cps locus, which contains 11 genes, abolished opacity.

177 nstrated that seven of the nine genes in the cps operon are essential for capsule production, indicat

178 he conserved proteins CpsABCD encoded in the cps operon, by developing knock-out and functional mutan

179 with the identity of four amino acids in the cps-encoded glycosyltransferase WcjA.

180 insertion of virulence factor pspC into the cps locus.

181 ed large recombination events, involving the cps IV operon and resulting in the expansion of serotype

182 of an approximately 20-kb sequence near the cps locus.

183 , that showed homology with the genes of the cps cluster, involved in polysaccharide biosynthesis, in

184 we showed that the full transcription of the cps genes not only depends on the core promoter immediat

185 o activator proteins and the promoter of the cps genes.

186 truncated galE2 locus and the capture of the cps island en bloc.

187 which the illegitimate recombination of the cps island into the galE allele of the cnl locus results

188 itative analysis showed that transfer of the cps locus had occurred at an elevated rate in beta-lacta

189 n the transcriptional characteristics of the cps locus in S. pneumoniae.

190 termined the transcriptional features of the cps locus in the type 2 virulent strain D39.

191 nucleotide polymorphism at codon 195 of the cps locus wciP gene.

192 by which EsaR governs the expression of the cps locus, which encodes functions required for stewarta

193 Transcription of the cps operon is controlled by at least two positive regula

194 minus of the first open reading frame of the cps operon was found to be homologous to proteins encode

195 ensing in the differential expression of the cps operon.

196 Here we report the characterization of the cps-6 gene, which appears to function downstream of, or

197 c rearrangements both within and outside the cps gene cluster, a mechanism which may be responsible f

198 It is likely that the cps genes are arranged in a single long operon that is a

199 The initial analysis revealed that the cps genes are cotranscribed from a major transcription s

200 estingly, promoter proximal genes within the cps cluster are significantly more acyl-homoserine lacto

201 inversion of the synthetic locus within the cps island during bacterial growth.

202 iously reported that cpsK, a gene within the cps locus of type III GBS, could complement a sialyltran

203 Thus, cps from oral Streptococcus strains can make acapsular p

204 In response to cps treatment of the immunosuppressive ovarian tumor env

205 re, we show this goal can be addressed using cps, an avirulent, nonreplicating uracil auxotroph strai

206 ion (BopD), adherence (Epb pili), virulence (cps loci, gelatinase, SprE) and antibiotic resistances (

207 nts per second/parts per billion and volume (cps/ppbv) at a mass resolution of >8000 m/Deltam (fwhm).

208 However, whereas cps transcription remained RcsA-dependent, ugd transcrip

209 Immunization with cps mutants demonstrated cross-protective immunity follo

210 Isolates with cps mutations showed improved survival following exposur

211 rts showing that intratumoral treatment with cps activated immune-mediated regression of established