ライフサイエンスコーパス: crude extract

1 late remained stable for 60 days, unlike the crude extract.

2 binding protein), and an adenovirus-infected crude extract.

3 ndem affinity purified RNA polymerase I from crude extract.

4 , purified TBP, or with protein from a yeast crude extract.

5 placed from its binding site by H4TF1 in the crude extract.

6 sence of yet unidentified factors present in crude extract.

7 DCM was collected and evaporated to yield crude extract.

8 he improved purification of Cy3-gal from the crude extract.

9 A-S) was only the active one followed by the crude extract.

10 ivity about 60 times higher than that of the crude extract.

11 d directly in plants expressing it or from a crude extract.

12 estion decreased 51-78% when compared to the crude extract.

13 orm, and n-butanol fractions, as well as the crude extract.

14 ciscana, across a range of concentrations of crude extract.

15 onal effect on transcriptional initiation in crude extracts.

16 lase activity for both purified proteins and crude extracts.

17 d to specifically bind biotinated Cin8p from crude extracts.

18 cificity as that seen intracellularly and in crude extracts.

19 erized after stabilization to proteolysis in crude extracts.

20 eacted with a 65-kDa protein in M. bovis BCG crude extracts.

21 wild type, and is catalytically inactive in crude extracts.

22 A1 led to an increase in the Vmax of CPA2 in crude extracts.

23 d a loss of Pab1p-stimulated PAN activity in crude extracts.

24 indicated the same molecular mass as that in crude extracts.

25 roducibility and standardization compared to crude extracts.

26 st with bioassay-guided fractionation of the crude extracts.

27 opies) with no negative interference by host crude extracts.

28 ed rapid DNA extraction methods that produce crude extracts.

29 ut none of them had higher activity than the crude extracts.

30 or detecting these low-abundance proteins in crude extracts.

31 fically retrieved PflB from Escherichia coli crude extracts.

32 ed with three different solvents to yield 72 crude extracts.

33 nsive spectral information of metabolites in crude extracts.

34 fenugreek and 13 from bitter melon in active crude extracts.

35 ty [0.072 (0.007) mg.mL(-1)] compared to the crude extract [0.026 (0.003) mg.mL(-1)].

36 dney bean) lectins, were coprecipitated from crude extracts, 0.05 to 0.4% crude protein, in a single

37 CdtA was present in two forms in crude extracts, 25 and 18 kDa; only the 18-kDa fragment

38 acid (EE-SA) (31.53%) and lower than in the crude extract (44%).

39 The ACs content reduced to 86.4% (crude extract), 60.9% (ACs-EEX), 36.0% (ACs-EES), 64.8%

40 per mille and -2.3 +/- 0.03 per mille) over crude extracts (-7.7 +/- 0.4 per mille and -3.4 +/- 0.02

41 s with the different components of a natural crude extract after being separated by a coupled HPLC co

42 es the feasibility of bioprofiling a natural crude extract after being separated in HPLC using microf

43 ative estimation of triterpenic acids in the crude extracts an NMR based methodology was used and com

44 opy was applied for metabolites profiling of crude extract and active fractions.

45 ificant seasonal and solvent effects for the crude extract and all the fractions except for the polar

46 docking studies of compounds observed in the crude extract and bioactive fractions had significant bi

47 ometry (LC-MS/MS) analysis of ethanolic seed crude extract and fraction M4 showed the presence of var

48 Crude extract and fractions were evaluated for their pot

49 s, antioxidant activities, and PPC among the crude extract and fractions, albeit to different extends

50 after in vitro digestion of a wheat gliadins crude extract and further characterized by LC-ESI-MS/MS.

51 The crude extract and its fractions were determined by measu

52 s; and (3) co-incubation of LPS with a pecan crude extract and its fractions.

53 haride (LPS); (2) pre-treatment with a pecan crude extract and its fractions; and (3) co-incubation o

54 Yields were measured for the crude extract and its neutral, organic, basic, polar and

55 In crude extract and partially purified preparations from E

56 In DNase I footprinting with both crude extract and purified protein, Mor protected Pm seq

57 ity of partially purified AtPAP15 from plant crude extract and recombinant AtPAP15 expressed in bacte

58 The antibacterial activity of crude extract and the separated metabolites were checked

59 The Kd values of SA binding to crude extract and to recombinant BiP were 45.2 and 54.6

60 The toxicity of the crude extract and two isolated components was evaluated

61 This enzyme was purified 90-fold from crude extracts and characterized.

62 specific binding properties as observed with crude extracts and correlated with the elution of a 36-k

63 Crude extracts and fractions were tested for elastase an

64 hondria, and showed comparable activities in crude extracts and in mitochondria isolated from transfe

65 cond, Bur1 and Bur2 coimmunoprecipitate from crude extracts and interact in the two-hybrid system; an

66 ith the purified enzyme and also tested with crude extracts and membrane fractions from bacteria and

67 Analysis of the reaction with crude extracts and purified components demonstrated that

68 ication, we fractionated adenovirus-infected crude extracts and tested them in an in vitro replicatio

69 iously described assays, one employing moeA- crude extracts and the other utilizing a defined system.

70 r DH had positive proliferative responses to crude extracts and two purified proteins, protein IV (83

71 +/- 0.03 (cell suspensions), -0.60 +/- 0.05 (crude extracts), and 1 (LinA1) or -1 (LinA2).

72 lations of alkaloid content, OMT activity in crude extracts, and OMT gene expression supported physio

73 ays were performed on whole tissue segments, crude extracts, and purified extracts.

74 SMG-1 and SMG-2 coimmunoprecipitate from crude extracts, and this interaction is maintained in sm

75 The antioxidant capacities of the crude extract, aqueous and ethyl acetate partitions of L

76 lic content and partition coefficient of the crude extracts are important parameters to control lipid

77 rk cartilage may work as a cancer retardant, crude extracts are ineffective.

78 or serum testing for allergen-specific IgE; crude extracts are the basis for most evaluations.

79 2 gene abolished an STRE-binding activity in crude extracts as judged by both gel mobility-shift and

80 detected in the stem (1105.14+/-243.10 mug/g crude extract), as SF was lower than the detection limit

81 on plants exposed to male fly emissions (or crude extracts), as well as enhanced induction of the ke

82 gels produced, after 2h, by chymosin and the crude extract at pH 3.

83 on of miraculin using IMAC was achieved with crude extract at pH 7, Tris-HCl binding buffer at pH 7 a

84 bserved compounds in both purified forms and crude extracts at an accuracy above 99%, while also corr

85 Crude extract based cell-free protein synthesis (CFPS) h

86 s that had been completely suppressed in the crude extract became readily detectable and quantifiable

87 Packaging events are as efficient as with crude extracts, but only if purified E. coli integration

88 NG protein inhibited base excision repair in crude extract by at least 90%.

89 apidly fractionate a multigram quantity of a crude extract by centrifugal partition extraction (CPE).

90 of CPC involves a multistep treatment of the crude extract by precipitation with ammonium sulphate, f

91 entiation of the antioxidant activity of the crude extract by up to six times (p < 0.05).

92 approximately 90%, only suppressed repair in crude extracts by a maximum of approximately 70%.

93 ta, ddCTP, decreased base excision repair in crude extracts by approximately 50%, whereas the Polalph

94 particles formed in E. coli were examined in crude extracts by electron microscopy.

95 d information on the chemical composition of crude extracts can be generated.

96 ivity and less odour, compared with those of crude extract (CE).

97 ied from Escherichia coli was incubated with crude extracts (CE) from strains RN6390 (rsbU) and SH100

98 me as well as the aminopeptidases present in crude extract cleaved preferentially Phe-pNA.

99 ductase were two to threefold lower in MP101 crude extracts compared with the BW25113 wild-type strai

100 e isolation of bioactive constituents from a crude extract containing close structural analogues rema

101 ed GDI as well as Escherichia coli (E. coli) crude extract containing GDI, suggest that this analogue

102 the L1 larvae of this nematode, exposure to crude extracts containing 2.5 muM avocadene, 4.3 muM per

103 Supershift analysis of EMSAs using crude extracts containing mycMga2p indicated that Mga2p

104 Crude extracts containing PceA--harboring either a nativ

105 P extract by i.p.injection indicate that the crude extract daidzin has approximately 10 times greater

106 es REV7 and, using immunoprecipitations from crude extracts, demonstrate that, in addition to the pol

107 stern blots with the recombinant proteins in crude extracts demonstrated that the monoclonal antibodi

108 the binding of RPA to single-stranded DNA in crude extracts derived from both C.B-17 and SCID cells.

109 le from that of the enzyme in a freshly made crude extract, even after storage of the pure sample for

110 Ethanolic seed crude extract exhibited the strongest radical scavenging

111 Crude extracts exhibited a high level of antimicrobial a

112 The fenugreek ethyl acetate crude extract (FGE3) demonstrated the highest antioxidan

113 Biochemical assay of crude extracts for 6-phosphogluconolactonase enzymatic a

114 Furthermore, enzyme in freshly-prepared crude extracts forms only very small amounts of GS-TriCH

115 ion in foods, confirmed that the brown algae crude extracts, fractions and pure components are compar

116 All crudes extracts, fractions as well as isolated compounds

117 ird-stage larvae (L3), molting L3 (mL3), and crude extract from adult males (M-OvAg) were compared to

118 Crude extract from applewood was obtained using ultrason

119 e residues, reaching 68.5 lipase U/g for the crude extract from fractions called frit.

120 tro with two different luminal proteins in a crude extract from sheep pancreas microsomes.

121 gents from natural products, we identified a crude extract from Tacca chantrieri that initiated Taxol

122 -chloro-l-tryptophan after reactivation with crude extract from the host strain, suggesting that an a

123 es of vhs protein were used in these assays: crude extract from virions or protein translated in a re

124 otein synthesis (CFPS) platform that employs crude extracts from a genomically recoded strain of Esch

125 A RDRP activity was identified in crude extracts from C. parvum sporozoites and products o

126 the addition of molybdenum in an assay using crude extracts from E. coli moeA(-) cells.

127 Crude extracts from Escherichia coli expressing AtzD hyd

128 medical practitioners began to believe that crude extracts from glands or other organs, when prescri

129 PPDs) exhibit multiple limitations: they are crude extracts from mycobacterial cultures with largely

130 in sensitivity allows 230 peaks detected in crude extracts from only a few pooled neuronal tissues a

131 ing lipid oxidation of an O/W emulsion, with crude extracts from overripe fruit and bush pruning resi

132 isotope dilution) of carotenoids present in crude extracts from plant tissues and whole cells; (iii)

133 ein reacted with proteins of similar size in crude extracts from Pseudomonas putida and Pseudomonas f

134 etabolism is established using LC-MS data of crude extracts from shaking flask fermentations.

135 c standards and mixtures thereof, as well as crude extracts from the known antibiotic producer Saccha

136 mRNA translation in crude extracts from the yeast Saccharomyces cerevisiae i

137 greater than 90% of the activity detected in crude extracts from wild-type yeast cells.

138 While the particle morphology visualized in crude extracts generally was the same as that visualized

139 her stability (half-life = 55 d) than in the crude extract (half-life = 43 d) and their stability inc

140 Since phosphatidylcholine species in crude extracts have been shown to cause ion suppression

141 riments using both purified RAG proteins and crude extracts have failed to detect trans cleavage of p

142 n of multiple antimicrobial compounds out of crude extracts, highlighting the practicality and high-t

143 Fractionation of these crude extracts identified replication factor C (RFC), pr

144 fluke-induced CCA, and out-performs parasite crude extract-IgG ICTs.

145 Encapsulated crude extract in porcine gelatin presented the smallest

146 arge numbers of VUS using small cultures and crude extracts in 96-well plates.

147 The changes in the composition of crude extracts in GID aliquots were followed by analysis

148 ation step is always performed in vivo or in crude extracts in the face of competition from natural a

149 that the Fe-S cluster synthesis observed in crude extracts in vitro may involve some of the componen

150 tify the possible bioactivity in the several crude extracts is highlighted.

151 The crude extract, its fractions, and compounds exhibited lo

152 ins, and antioxidant activities, followed by crude extract, LWM-FP, and LMW, respectively.

153 f both MgATP and wild-type Fe protein to the crude extracts made by A. vinelandii UW97.

154 rify naturally abundant metalloproteins from crude extracts mainly from plants but also from bacteria

155 Compared with crude extract, normal hexane fractions (NHFs) have a rem

156 In this work crude extracts obtained from the edible part of Chamelea

157 uring in vitro physiological metabolism in a crude extract of bacteriophage T7-infected cells.

158 Wistar rats received a single dose of crude extract of blueberry obtained using NADES (CE-NADE

159 activated in vitro by factors present in the crude extract of E. coli and to a much smaller extent in

160 Four enzymatic activities in the crude extract of E. coli were found that can provide sul

161 The crude extract of Eremophila rugosa was subjected to PLMN

162 and OAG) were tentatively identified in the crude extract of grapefruit seeds by ESI mass spectromet

163 The crude extract of Hemimycale sp. marine sponge was evalua

164 ydrophobic interaction chromatography of the crude extract of mucoid P. aeruginosa 8821, a CF isolate

165 ocess for the purification of miraculin from crude extract of S. dulcificum.

166 A crude extract of Schinus terebinthifolia leaves exhibite

167 Using a crude extract of Streptomyces collinus, we have resolved

168 (1), trinactin (2), and tetranactin (3) in a crude extract of Streptomyces sp. AMC 23 in the precurso

169 55 and 585 helped to identify nigericin in a crude extract of Streptomyces sp. Eucal-26 by means of p

170 In an assay for cytotoxicity, we found the crude extract of the cyanobacterium to be much more pote

171 This insertion reaction required crude extract of the DeltanifHDK A. vinelandii strain CA

172 as the microtubule-active constituent in the crude extract of the Mountain torchwood, Amyris madrensi

173 ectrum of recombinant PR1 was similar to the crude extract of the native luciferase, suggesting that

174 Bioassays with the crude extract of the pigmented trichome and a three-comp

175 ference standards, and (iii) LC-QTOF data of crude extracts of 10 strains of laboratory grown culture

176 Crude extracts of a PHO13-overexpressing strain showed a

177 tion factor was detected on the PC arrays in crude extracts of all stages of the seedling cotyledons,

178 Crude extracts of Ataulfo exhibited polyphenol oxidase (

179 f hMS holoenzyme also were examined by using crude extracts of baculovirus-infected insect cells cont

180 erall, the findings provide information that crude extracts of brown edible seaweeds, phenolic compou

181 uctase activity (2 to 39 nmol min-1 mg-1) in crude extracts of C. thermoaceticum.

182 stingly, the carbonyl content of proteins in crude extracts of cells harvested after 48 h of stationa

183 al RNA processing in immunoprecipitates from crude extracts of cells.

184 e, RNase MRP RNA, in immunoprecipitates from crude extracts of cells.

185 s capable of reliably detecting the virus in crude extracts of CPMV-infected leaves and can therefore

186 Crude extracts of cultured parasites, prepared simply by

187 Crude extracts of defatted seeds were analysed by means

188 In contrast, deacetylase activity in crude extracts of E. coli was insensitive to EDTA, and t

189 of sensitivity employed Eu3 was detected in crude extracts of embryos but not non-embryonic tissues

190 When crude extracts of French-pressed or osmotically shocked

191 Crude extracts of Geobacter sulfurreducens catalyzed the

192 teins, pyronin-modified Rnase A was added to crude extracts of human HeLa cells.

193 or have only a subarthritogenic effect, and crude extracts of human osteoarthritic cartilage induced

194 pullulanase or D-enzyme could be detected in crude extracts of leaves of the mutant.

195 bstrates were also efficiently methylated in crude extracts of loblolly pine secondary xylem.

196 Km(app) values obtained in crude extracts of male or female rat liver and post-benz

197 rified Sco1p sediments identical to Sco1p in crude extracts of mitochondria from wild type yeast or f

198 The CO oxidation activity in crude extracts of nitrate (30 mM)-supplemented cultures

199 n of excess heavy metal, as was the case for crude extracts of P. aeruginosa.

200 A screening of marine sponges revealed that crude extracts of Psammocinia sp. exhibited potent 15-hL

201 the cbb(I) promoter region were detected in crude extracts of R. sphaeroides.

202 ) but also the major ecdysteroids present in crude extracts of Silene otites, Silene nutans, and Sile

203 The enzyme was purified from crude extracts of soybean root nodules approximately 100

204 ic activity could be detected in vitro using crude extracts of stationary phase cultures, but was abs

205 In the present report, we show that crude extracts of Streptococcus agalactiae catalyze the

206 lel to these experiments, in vitro assays on crude extracts of T. pseudonana demonstrated mean inhibi

207 ration of intact allergens, often comprising crude extracts of the allergen.

208 Comparison of the protein profile of crude extracts of the B. fragilis strains revealed that

209 As isolated and as present in crude extracts of the files, this xanthine dehydrogenase

210 In addition, crude extracts of the mutant failed to convert 4-hydroxy

211 In addition, Sll1951 was prominent in crude extracts of the wild type, indicating that it is a

212 Crude extracts of this strain showed no aspartyl peptida

213 Incubation of CGA with crude extracts of tomato fruits led to the formation of

214 Immunoblotting analysis of crude extracts of transfected fibres demonstrated the sy

215 ever, hydroxylase activity was detectable in crude extracts of vegetative tissues.

216 luated the antimicrobial capabilities of the crude extracts of wild ferns and the sun protection fact

217 When crude extracts of wt, HypA:kan and HypB:kan were separat

218 Analyses of crude extracts of yeast expressing L183P-hGALE demonstra

219 , we propose (i) a one-step fractionation of crude extracts on P11 phosphocellulose, followed by (ii)

220 Previously, intensive in vitro studies with crude extract or purified enzyme concluded that the atta

221 riments employing (i) cell suspensions, (ii) crude extracts, or (iii) LinA1 and LinA2 enzymes of stra

222 Increased levels of molecular chaperones in crude extracts, particularly DnaJ, indicated a rather in

223 n, where the influence of binding buffer pH, crude extract pH and imidazole concentration in elution

224 All investigations were performed using crude extracts possessing hundreds of phyto-substances.

225 izable and easily accessible high-throughput crude extract preparation method for CFPS based on sonic

226 geting vankyrin detected a 19-kDa protein in crude extracts prepared from the 3 days p.p. fat body.

227 Electrophoretic mobility shift assays using crude extracts prepared from wild-type and argP-defectiv

228 Substances, or crude extracts, produced by worms and responsible for th

229 c preparations from black chokeberry fruits: crude extract, purified extract standardized to 20% and

230 ng purification it was observed that NifW in crude extracts ran above the predicted molecular weight

231 ation of a chemically complex Rhodiola rosea crude extract (RCE).

232 The soluble pool of FP21 from crude extracts resolves chromatographically into two fra

233 .05+/-246.18 and 111.94+/-16.49 mug/g in the crude extract, respectively), while only SE was detected

234 Its ethyl acetate crude extract showed moderate antibacterial activity aga

235 Plant crude extracts showed 6-7 fold higher enzyme activity th

236 It was found that 30 out of 106 crude extracts showed more than 80% inhibition of the al

237 Preliminary screening in Escherichia coli crude extracts showed that their presence during protein

238 Such covalent labeling is not suitable for crude extracts such as native nanodiscs directly obtaine

239 , exhibited 12% of the wild-type activity in crude extracts, suggesting that Mn remains bound; howeve

240 tro was primed by DnaG primase, whereas in a crude extract system that had not been fractionated, it

241 The optimal conditions were: a ratio of crude extract/t-butanol of 0.87 (v/v), saturation in amm

242 a Doehlert design with 3 variables (ratio of crude extract/t-butanol, the ammonium sulphate saturatio

243 e have detected several proteins in S. pombe crude extracts that bind to the oligonucleotide and ars3

244 er to evaluate the assay for glycoprotein in crude extract, the glycoprotein was separated by SDS-PAG

245 In crude extracts, the hydroxylaminobenzene mutase was stab

246 he precursor ions of all other lipids in the crude extracts, thereby enabling their unambiguous assig

247 ene-free approach by applying TRV-containing crude extracts through foliar spraying, eliminating the

248 (30 % (w/v) ammonium sulfate, 1.0:1.5 (v/v), crude extract to t-butanol ratio, pH 9.0, and 30 degrees

249 he ability of both purified RAG proteins and crude extracts to cleave DNA substrates in trans is a fu

250 d adsorption of the biotinylated module from crude extracts to immobilized streptavidin.

251 of the native PKA has also been detected in crude extracts using kemptide as a substrate.

252 emical profile, was produced from grape seed crude extract ( Vitis vinifera; enriched grape seed extr

253 onger isotope fractionation was observed for crude extracts vs intact cells of Sulfurospirillum multi

254 with a methanol/chloroform solution, and the crude extract was directly analyzed by DESI-MS, with a t

255 The crude extract was evaluated for the content and profile

256 Additionally, the cytotoxic activity of the crude extract was explained on the molecular level, wher

257 The crude extract was first separated by low pressure liquid

258 Crude extract was obtained from intestines of fish Nile

259 Moreover, a skin prick test using the crude extract was positive for A. simplex but negative f

260 A phenolic crude extract was prepared from pecans and separated by

261 The crude extract was prepared with acetone (60% v/v) and pu

262 The bioactive fraction in the crude extract was revealed by TLC-bioautography at R(f)

263 Neither of the crude extracts was able to inhibit lipid oxidation of th

264 on with homodimeric Fe proteins contained in crude extracts was accomplished by construction of a sev

265 Also, the antioxidant activity of crude extracts was assessed by in-vitro determinations:

266 Crude extracts were prepared from residues of Rubus glau

267 Fruit pomace crude extracts were the best controlling lipid oxidation

268 Crude extracts were used for characterization of enzyme

269 i challenged with 41 known antibiotics and 9 crude extracts while depositing 122 transcriptomes uniqu

270 This method enables clean-up of crude extracts within 18min and screening and confirmati