ライフサイエンスコーパス: crystallin

1 subpopulation marked by the expression of mu-crystallin.

2 treated with the heat shock protein alpha-B crystallin.

3 t shock protein (HSP) homolog of human alpha-crystallin.

4 duction was inhibited by knockdown of alphaB-crystallin.

5 n but was inhibited by suppression of alphaB-crystallin.

6 ficient to inhibit aggregation of lens gamma-crystallin.

7 ch as p62, heat shock protein B8, and alphaB-crystallin.

8 merization was effectively reduced by alphaB-crystallin.

9 ract in a nonspecific manner with the alphaB-crystallin.

10 ses aggregation of the lens chaperone alphaB crystallin.

11 iciently in the presence of chaperone alphaB-crystallin.

12 c-Maf, Prox1, and alphaA-, alphaB-, and beta-crystallins.

13 rkers Lhx2, Pax6, Jag1, E-cadherin and gamma-crystallins.

14 ression of the lens structural proteins, the crystallins.

15 eased levels of the stress-responsive alphaB-crystallins.

16 gregation of various cataract-causing mutant crystallins.

17 h to treating cataracts by stabilizing alpha-crystallins.

18 of the major chaperones, alphaA- and alphaB-crystallins.

19 es for good predictive binding to the alphaB-crystallins.

20 MS that these peptides are from a variety of crystallins.

21 as been shown to be extensive throughout the crystallins.

22 l experiments on in vitro alphaB- and gammaD-crystallin, 2D IR spectroscopy can identify the highly o

23 tide from water-insoluble aggregates, alphaA crystallin (66)SDRDKFVIFL(isoAsp)VKHF(80), results in en

24 codon variants in the gene encoding gamma-B crystallin, a mammalian eye-lens protein, modulate the r

25 idual unfolding pathways of the human gammaD-crystallin, a multidomain protein that must remain corre

26 pairs exosome secretion by decreasing alphaB-crystallin, a protein that is expressed mainly in glial

27 strocytes decreased the expression of alphaB-crystallin, a small heat shock protein that is enriched

28 st abundant sHSPs in human tissue are alphaB-crystallin (ABC) and HSP27; here we present high-resolut

29 Both alphaA- and alphaB-crystallin act as chaperones and anti-apoptotic proteins

30 yed by luciferase refolding and human gammaD-crystallin aggregation suppression and refolding.

31 orescence intensity of Smad7 and the corneal crystallin aldehyde dehydrogenase 3A1.

32 dopsis reductase ornithine cyclodeaminase/mu-crystallin, alias SYSTEMIC ACQUIRED RESISTANCE-DEFICIENT

33 small heat shock proteins alphaA- and alphaB-crystallin (alphaA/alphaB(-/-)) develop cataracts.

34 alphaB-Crystallin (alphaB) and HSP27 are the two most widely di

35 sly that the small heat shock protein alphaB-crystallin (alphaB) is exported out of the adult human r

36 chanism by which two canonical sHsps, alphaB-crystallin (alphaB-c) and Hsp27, interact with aggregati

37 Crystallin-alphaB (CryAB) is a small heat shock protein

38 AlphaB-crystallin (alphaBC) is a small heat shock protein that

39 alphaB-crystallin (alphaBc) or HspB5 is a well-characterized me

40 observe the chaperone action of human alphaB-crystallin (alphaBc, HSPB5).

41 alphaB-crystallin also prevented the unfolding and nonfibrillar

42 teins HSP27 (also known as HSPB1) and alphaB-crystallin (also known as HSPB5), which stabilize nuclea

43 The chaperone proteins, alpha-crystallins, also possess antiapoptotic properties.

44 Here we report that alphaB-crystallin, an antiapoptotic molecular chaperone implica

45 gomers, which are larger than reduced alphaA-crystallin and destabilized against unfolding, are activ

46 in age-related cataracts are bound by alpha-crystallin and form light-scattering HMW aggregates.

47 alphaB-crystallin and HSP27 are mammalian intracellular small h

48 eptide (70)KFVIFLDVKHFSPEDLTVK(88) in alphaA-crystallin and the peptide (73)DRFSVNLDVKHFSPEELKVK(92)

49 ividually for the expression of all known 17 crystallins and 78 other relevant genes using a Biomark

50 olecular mechanisms are applicable for other crystallins and genes highly expressed in terminally dif

51 been shown that HspB1 (Hsp27), HspB5 (alphaB-crystallin), and HspB6 (Hsp20) can form hetero-oligomers

52 isoforms, phospholamban, dystrophin, alphaB-crystallin, and calsequestrin 2 as novel maturation-asso

53 oligomers (HspB1/Hsp27, HspB3, HspB4/alphaA-crystallin, and HspB5/alphaB-crystallin) are promiscuous

54 morphogenetic protein 4 up-regulated alphaB-crystallin, and its EMT induction was inhibited by knock

55 ptide ratios of 248 lens proteins, including Crystallin, Aquaporin, Collagen and enzymes that catalyz

56 of Hsp27 and the terminal regions of alphaB-crystallin are important for delaying amyloid fibril nuc

57 gammaD- and gammaS-crystallin are two major monomeric crystallins of the hu

58 n vivo and their subsequent interaction with crystallins are responsible, in part, for protein aggreg

59 3, HspB4/alphaA-crystallin, and HspB5/alphaB-crystallin) are promiscuous chaperones, whereas the chap

60 Taken together, our findings point to alphaB-crystallin as a novel regulator of anoikis resistance th

61 Despite its oligomeric nature, Rh of alphaB-crystallin as derived from both NMR methods is found to

62 ptide (73)DRFSVNLDVKHFSPEELKVK(92) in alphaB-crystallin as mini-chaperones.

63 We show here that HSP27 and alphaB-crystallin associated with immunoglobulin-like (Ig) doma

64 ation in Cryba1 (the gene encoding betaA3/A1-crystallin), astrocytes exhibit decreased Notch signalin

65 ised of nine protein spots containing betaB2-crystallin at 10-40-fold higher abundance and three prot

66 ents on lens tissue show colloidal gels of S-crystallins at all radial positions.

67 signaling up-regulates expression of alphaA-crystallin both directly and indirectly via up-regulatio

68 further enhanced by overexpression of alphaB-crystallin but was inhibited by suppression of alphaB-cr

69 he disorder "solid" carbon nanofibers-->well crystallined carbon nanofibers-->bent graphitic sheets--

70 c of the desmin-related cardiomyopathies and crystallin cardiomyopathic diseases.

71 egates as a result of a mutant CryAB (alphaB-crystallin) causative for human desmin-related cardiomyo

72 The results suggest that the alpha-crystallin chaperone peptides could be used as therapeut

73 erol can restore vision by binding to alphaB-crystallin chaperone protein to dissolve or disaggregate

74 est that AGE-mediated cross-linking of alpha-crystallin-client complexes could contribute to lens agi

75 he stability of the alphaA-crystallin-gammaD-crystallin complex for up to 12 days and observed that t

76 sses and pathways in the lens including lens crystallins, connexins, growth factors, membrane protein

77 Human alphaA-crystallin contains two cysteines, which can form an int

78 e to aggregation, which, in the case of lens crystallin, contributes to cataract formation.

79 Overexpression of alphaB-crystallin could alleviate the deficient exosome release

80 ncogene c-Maf regulates expression of alphaA-crystallin (Cryaa) through binding to its promoter and d

81 ntified a class of molecules that bind alpha-crystallins (cryAA and cryAB) and reversed their aggrega

82 Mutated alphaB-crystallin (CryAB(R120G)), when expressed only in cardio

83 The small heat shock protein alphaB-crystallin (CRYAB) has been implicated in multiple scler

84 ted with increased expression in alpha-basic crystallin (CRYAB), which has previously bound VEGF.

85 cyte-specific expression of a mutated alphaB crystallin, CryAB(R120G).

86 ssion of the schizophrenia-related gene beta crystallin (Crybb1).

87 Here, we identify and validate the mu-crystallin (crym) gene as a high-fidelity marker of the

88 Nanodelivery of alphaB-crystallin-derived mini-chaperone peptide offers an alte

89 as to investigate whether 19 to 20-mer alpha-crystallin-derived mini-chaperone peptides (alpha-crysta

90 ellular uptake of fluorescein-labeled, alpha-crystallin-derived mini-peptides and recombinant full-le

91 e presence of 19 to 20-mer alphaA- or alphaB-crystallin-derived or scrambled peptides.

92 imal while a time-dependent uptake of alphaB-crystallin-derived peptide was observed.

93 rat, mutation in the gene encoding betaA3/A1-crystallin disrupts both Notch signalling in astrocytes

94 tures, the phase-separated droplets of gamma-crystallin dissolve into a homogeneous solution at as lo

95 n binding groove within the structured alpha-crystallin domain (ACD) and to sites within the enigmati

96 While the alpha-crystallin domain (ACD) dimer of sHSPs is the universal

97 ylation motif around Ser16, and a core alpha-crystallin domain (ACD) responsible for dimerisation.

98 he structured core of the protein, the alpha-crystallin domain (ACD), forms dimers and can prevent th

99 t sHSP structure beyond its structured alpha-crystallin domain (ACD), which is flanked by disordered

100 nd function of HSP27 and its conserved alpha-crystallin domain (ACD).

101 sHSPs comprise a conserved alpha-crystallin domain flanked by variable N- and C-terminal

102 also identified a novel C-terminal betagamma-crystallin domain in FgFCO1 devoid of calcium binding mo

103 A betagamma-crystallin domain is formed of two Greek key motifs, acc

104 ts strongly suggest that Hspb8 and its alpha-crystallin domain might act as pleiotropic prosurvival f

105 so discovered that the truncated form of the crystallin domain of Hspb8 was sufficient to affect cell

106 total, these data imply that the core alpha-crystallin domain of the sHSPs is a platform for flexibl

107 IDM2 encodes an alpha-crystallin domain protein in the nucleus.

108 histone acetyltransferase IDM1 and the alpha-crystallin domain proteins IDM2 and IDM3.

109 We conclude that although the isolated alpha-crystallin domain retains some chaperone activity agains

110 but also tethered by contacts with the alpha-crystallin domain shell.

111 sHSPs share an 'alpha-crystallin domain' with a beta-sandwich structure and a

112 e defined by a conserved beta-sandwich alpha-crystallin domain, flanked by variable N- and C-terminal

113 verse chaperone family that shares the alpha-crystallin domain, which is flanked by variable, disorde

114 Paralleling the diversity in betagamma-crystallin domains, these motifs also show great diversi

115 uctures for the P23T + R36S mutant of gammaD-crystallin, each with opposite solubility behavior: one

116 Furthermore, inhibition of alphaB-crystallin enhanced monotetraubiquitination of SMAD4, wh

117 Overexpression of alphaB-crystallin enhanced nuclear translocation and accumulati

118 ing motifs are necessary for Ciona betagamma-crystallin expression, and narrow down the likely factor

119 ell proliferation and uniquely affected beta-crystallin expression.

120 d with SB, altered LECs' morphology and beta-crystallin expression.

121 Chemical damage to proteins of the crystallin family, accumulated over a lifetime, leads to

122 y taking advantage of its unique IgE-binding crystallin fold, activates basophils by a novel, cross-l

123 le loop, which is unique to the IPSE/alpha-1 crystallin fold.

124 he oxidation-mimicking W42Q mutant of gammad-crystallin formed non-native polymers starting from a na

125 will enable in vivo testing of the roles of crystallin fragments in protein aggregation.

126 The accumulation of crystallin fragments in vivo and their subsequent intera

127 tions of the bovine eye lens protein beta(H) crystallin from dilute conditions up to dynamical arrest

128 Comparison of crystallins from five species: two aquatic and three ter

129 Additionally, crystallin-gamma subunits and serotonin-related genes we

130 say, we examined the stability of the alphaA-crystallin-gammaD-crystallin complex for up to 12 days a

131 The fish eye lens protein gammaM7-crystallin (gammaM7) is found in vivo at extremely high

132 In the human eye lens, gammaS-crystallin (gammaS-WT) forms a densely packed transparen

133 p300, and recruited a repressor, Sp3 to beta-crystallin gene promoters, to negatively regulate their

134 ver, a direct link between FGF signaling and crystallin gene transcriptional machinery remains to be

135 ctivity for the Ciona intestinalis betagamma-crystallin gene, which drives expression in the lens of

136 ation of Sp1 with the enhancer of the alphaB-crystallin gene.

137 ystem to specifically disrupt the two alphaB-crystallin genes, alphaBa and alphaBb We observed lens a

138 e in abundance after deletion of these alpha-crystallin genes.

139 The alpha-crystallin glass transition could have implications for

140 Overexpression of alphaA-crystallin had no significant effects on the penetrance

141 Therefore, enhanced alphaB-crystallin has an essential role in TSC1/2 complex defic

142 in the human small heat shock protein alphaB-crystallin have been implicated in autosomal cataracts a

143 A number of mutations of lens crystallins have been associated with dominant cataracts

144 The crystallins have relatively high refractive increments c

145 C-terminal region is a determinant of alphaA-crystallin heterogeneity.

146 oblot analyses revealed decreases in beta-B1-crystallin, Hsp-90, spectrin and filensin.

147 vities of various constructs of human alphaB-crystallin (HSPB5) and heat-shock 27-kDa protein (Hsp27,

148 pared the chaperone activity of human alphaB-crystallin (HSPB5) with HSPB5 variants that mimic states

149 with sHsps, including Hsp27 (HspB1), alphaB-crystallin (HspB5), Hsp22 (HspB8), and Hsp20 (HspB6).

150 Here we show that loss of betaA3/A1-crystallin in astrocytes does not impede Notch ligand bi

151 e that mHtt reduces the expression of alphaB-crystallin in astrocytes to decrease exosome secretion i

152 In addition, silencing alphaB-crystallin in metastatic carcinoma cells reduced the num

153 highlight the potent effectiveness of alphaB-crystallin in preventing beta2m aggregation at the vario

154 e uncovered another critical role for alphaB-crystallin in providing stress tolerance to the heart.

155 r work illuminates the involvement of alphaB-crystallin in stress tolerance of the heart presumably t

156 However, the role of alphaB-crystallin in subretinal EMT and fibrosis is unknown.

157 , confocal laser microscopy revealed alpha-B crystallin in subsarcolemmal aggresomes.

158 The core function of gammaS-crystallin in the eye lens may be precisely its capacity

159 alpha-Crystallin in the lens binds to other proteins and preve

160 ty in vitro, the in vivo functions of alphaB-crystallin in the maintenance of both lens transparency

161 cal role of the chaperone activity of alphaB-crystallin in the maintenance of lens transparency.

162 , we investigate the eye-lens protein gammaB-crystallin in the ribosomal exit tunnel.

163 ved a restoration of the normal level alphaB-crystallin in the striatum of the BACHD/GFAP-CreERT2 mic

164 Overexpression of betaA3/A1-crystallin in those same astrocytes restored V-ATPase ac

165 uction in anoikis, we stably silenced alphaB-crystallin in two different metastatic carcinoma cell li

166 eak affinity to the resident chaperone alpha-crystallin in vitro To better understand the mechanism o

167 By contrast, denatured crystallins in age-related cataracts are bound by alpha-

168 dhesion proteins, cytoskeletal proteins, and crystallins in lens opacities caused by the absence of t

169 implications of Ca(2+) binding to betagamma-crystallins in mediating biological processes are yet to

170 folding rate constants and half-lives of the crystallins in the absence of the denaturant.

171 Amorphous aggregation of gamma-crystallins in the eye lens causes cataract, a widesprea

172 the accumulation of UV-damaged human gammaD-crystallins in the eye lens.

173 ndent on the stability and solubility of the crystallins in the fiber cells.

174 Strikingly, silencing alphaB-crystallin increased matrix detachment-induced caspase a

175 n induced EMT, whereas suppression of alphaB-crystallin induced a mesenchymal-epithelial transition.

176 In vitro, overexpression of alphaB-crystallin induced EMT, whereas suppression of alphaB-cr

177 is both necessary and sufficient for alphaB-crystallin induction by matrix detachment.

178 onstitutive ERK activation suppresses alphaB-crystallin induction during matrix detachment.

179 xamine the functional consequences of alphaB-crystallin induction in anoikis, we stably silenced alph

180 Silencing of alphaB-crystallin inhibited multiple fibrotic processes, includ

181 ed granules, we find the lens protein Omega- crystallin interfacing tightly with pigment molecules.

182 alpha-Crystallin is a member of the small heat-shock protein (

183 The small heat shock protein alphaA-crystallin is a molecular chaperone important for the op

184 alphaB-Crystallin is a protein chaperone with anti-apoptotic an

185 Thus, alphaB-crystallin is an important regulator of EMT, acting as a

186 The small heat shock protein alphaB-crystallin is an oligomeric molecular chaperone that bin

187 The V75D mutant of gammaD-crystallin is associated with congenital cataract in mic

188 gammaD-crystallin is concentrated in the oldest lens fiber cell

189 iber cells, the lens nucleus, whereas gammaS-crystallin is concentrated in the younger cells of the l

190 ics simulations that the stability of gammaD-crystallin is greatly reduced by the conversion of trypt

191 Quite unexpectedly, the LLPS of gamma-crystallin is much more sensitive to pressure than folde

192 .e., in the cytosol of astrocytes, betaA3/A1-crystallin is necessary for the phosphorylation of STAT3

193 king finding was a cohort of lens-associated crystallin isoform mRNAs lost from the eif3ha morphant p

194 of a phenotype previously reported in alphaB-crystallin knock-out mice and suggests that the elevated

195 l retinal pigment epithelium cells in alphaB-crystallin knockout mice compared with wild-type mice.

196 ch is equivalent to a mutant of human alphaB-crystallin linked to cardiomyopathy.

197 betagamma-Crystallins make a separate class of Ca(2+)-binding prot

198 Unexpectedly, like Crystallins, many enzymes with relatively high abundance

199 We show that STAT3 and betaA3/A1-crystallin may co-regulate each other in astrocytes.

200 ated tumorigenesis, and inhibition of alphaB-crystallin may complement the current therapy for TSC.

201 ransparency, our results suggest that alphaA-crystallin may not be efficient to inhibit aggregation o

202 lins suggesting that the primary sequence of crystallins may be specifically enriched with amino acid

203 n two-domain architecture of vertebrate lens crystallins may be the origin of their high kinetic as w

204 cantly lower concentrations than free alphaB-crystallin mini-chaperone peptide.

205 ctone (PCL) nanoparticle encapsulated alphaB-crystallin mini-chaperone peptides from H2O2-induced cel

206 PCL nanoparticles containing alphaB-crystallin mini-chaperone were also taken up and protect

207 allin-derived mini-chaperone peptides (alpha-crystallin mini-chaperone) are antiapoptotic, and to ide

208 alphaA- and alphaB-crystallin mini-chaperones offer protection to hfRPE cel

209 xidative stress and either alphaA- or alphaB-crystallin mini-chaperones remained viable and showed ma

210 Crystallin modifications and cleavage were quite dramati

211 tra collected at various R3Abeta2m to alphaB-crystallin molar subunit ratios, it is concluded that th

212 yproteins containing two neighboring HgammaD-crystallin monomers, we captured an anomalous misfolded

213 f aggregates formed by the P23T human gammaD-crystallin mutant associated with congenital cataracts.

214 cyte-specific expression of a mutant alpha-B-crystallin, mutant CryAB (CryAB(R120G)), which shows imp

215 The alphaB-crystallin mutants exhibited hypersusceptibility to deve

216 we transgenically expressed different gammaD-crystallin mutants in the zebrafish lens and observed a

217 particular interest were gammaB- and gammaD-crystallin mutants linked to dominant cataracts in mouse

218 y the mechanism of aggregation of two gammaD-crystallin mutants, W42R and W42Q: the former a congenit

219 ted formation of amorphous deposits by other crystallin mutants.

220 The gamma-crystallins of the eye lens nucleus are among the longes

221 nd gammaS-crystallin are two major monomeric crystallins of the human lens.

222 We determined the structures of human alphaA-crystallin oligomers by combining cryo-electron microsco

223 We also generated complexes of human alphaA-crystallin or alphaB-crystallin with alcohol dehydrogena

224 uired the downstream expression of the alpha-crystallin ortholog HSP-16.48 Using a combination of pha

225 A scrambled alphaA-crystallin peptide produced no such effects.

226 To achieve this goal, the alphaB-crystallin peptide was re-engineered into a protein poly

227 te isomerization, we investigated two alphaA crystallin peptides (52)LFRTVLDSGISEVR(65) and (89)VQDDF

228 n the isomerization of aspartate residues in crystallin peptides differentially results in peptides a

229 water-soluble protein extracts primarily to crystallin peptides in high-molecular weight aggregates

230 The alphaA- and alphaB-crystallin peptides inhibited stress-induced aggregation

231 Both the acetyl and native crystallin peptides inhibited stress-induced apoptosis i

232 To determine what role this crystallin plays after peripheral nerve damage, we found

233 alphaB-crystallin plays multiple roles in age-related macular d

234 ined the ability to differentiate into gamma-crystallin-positive lentoids by high-dosage bFGF treatme

235 entially under acidic conditions, and alphaB-crystallin prevented this aggregation.

236 iomyocyte stiffness was corrected by alpha-B crystallin probably through relief of titin aggregation.

237 Gamma-B crystallins produced from mRNAs with changed codon bias

238 Surprisingly, wild-type gammad-crystallin promoted W42Q polymerization in a catalytic m

239 eptide derived from residues 73-92 of alphaB-crystallin protects human retinal pigment epithelial (RP

240 d kinase (ERK) activity and increases alphaB-crystallin protein and messenger RNA (mRNA) levels.

241 imics matrix detachment by increasing alphaB-crystallin protein and mRNA levels, whereas constitutive

242 eus and increased expression of a particular crystallin protein group.

243 ynamics of a model calcium-binding betagamma-crystallin protein, Protein S, and elaborate on its inte

244 The multidomain calcium-binding betagamma-crystallin proteins are particularly important because t

245 The human lens is comprised largely of crystallin proteins assembled into a highly ordered, int

246 cts are caused when damage to the major lens crystallin proteins causes their misfolding and aggregat

247 in this fashion by examination of long-lived crystallin proteins extracted from a sheep eye lens.

248 ng and short-range order interactions of the crystallin proteins in fiber cells lacking organelles.

249 UV light and other factors damage crystallin proteins in the eye lens, resulting in catara

250 Cataracts are formed by the aggregation of crystallin proteins in the eye lens.

251 lear eye lens brought about by deposition of crystallin proteins in the lens fiber cells.

252 Many in vitro studies have established that crystallin proteins precipitate into aggregates that con

253 Crystallin proteins, the dominant constituents of the ey

254 For alpha-crystallin proteins, the sites that undergo the greatest

255 mparative discussion with results from other crystallin proteins, we suggest an interesting common pa

256 g common pathway for dynamical arrest in all crystallin proteins, with potential implications for the

257 from an evolutionary radiation of globular S-crystallin proteins.

258 ntiation characterized by high expression of crystallin proteins.

259 ht into the structure and function of alphaA-crystallin provides a basis for understanding its role i

260 Our results also suggest that alphaB-crystallin represents a promising molecular target for a

261 Importantly, overexpression of alphaB-crystallin rescues defective exosome release from HD ast

262 gment might exert catalytic activity against crystallins, resulting in the accumulation of distinct L

263 The application to chicken gammaS-crystallin reveals weak monomer-dimer self-association w

264 Furthermore, and most importantly, alphaB-crystallin reversibly dissociated beta2m oligomers forme

265 alpha-B crystallin shifted the Fpassive-sarcomere length relatio

266 nd analysis show that aqueous eye lens alpha-crystallin solutions exhibit a glass transition at high

267 existence curves for lysozyme and gamma IIIa-crystallin solutions in respective buffers.

268 scattering liquid structure data from alpha-crystallin solutions over an extended range of protein c

269 ilute and concentrated bovine eye lens alpha-crystallin solutions, using small-angle X-ray scattering

270 These trends are also seen in S-crystallins suggesting that the primary sequence of crys

271 at the same time as Hsp22, Hsp27, or alphaB-crystallin, suggesting that it might physically bring th

272 PSE/alpha-1 is a new member of the betagamma-crystallin superfamily.

273 ion of and refold the model substrate gammad-crystallin, suppress aggregation of mutant huntingtin, a

274 ess under acidic stress, but HSP27 or alphaB-crystallin suppressed this stiffening.

275 Disruption of alphaB-crystallin suppressed Tsc2-null cell proliferation and t

276 n of recombinant forms of alphaA- and alphaB-crystallin that mimic isomerized residues deviated from

277 Quiescent keratocytes are thought to produce crystallins that match the refractive index of their cyt

278 meric interfaces in human alphaA- and alphaB-crystallin, the most abundant chaperone proteins in the

279 diators that could be regulated by betaA3/A1-crystallin to modulate both GFAP and VEGF.

280 betagamma-Crystallin-type double clamp (N/D)(N/D)XX(S/T)S motif is

281 re on the LLPS of the eye-lens protein gamma-crystallin using UV/vis and IR absorption, fluorescence

282 quences of amino acid isomerization in alpha-crystallins using mass spectrometry, molecular dynamics

283 and 2KLR) and one R120G mutant (2Y1Z) alphaB-crystallins using standard MOE(TM) (Molecular Operating

284 approach toward a glass transition at alpha-crystallin volume fractions near 58%.

285 The augmented alphaB-crystallin was critical for the migration, invasion and

286 -peptides and recombinant full-length alphaB-crystallin was determined in confluent hfRPE.

287 (beta2m) and the molecular chaperone alphaB-crystallin was investigated by thioflavin T fluorescence

288 Uptake of full-length alphaB-crystallin was minimal while a time-dependent uptake of

289 alphaB-crystallin was prominently expressed in subretinal fibro

290 alphaB-crystallin was transcriptionally activated by mTOR compl

291 We report here that expression of alphaB-crystallin was upregulated in Tsc1-/- or Tsc2-/- mouse e

292 The Greek key motif in betagamma-crystallins was compared with that in other proteins, us

293 nsequently, expression of alphaB- and alphaA-crystallins was not initiated.

294 pression of lens-specific genes such as beta-crystallins, was positively regulated by SUMO1 but negat

295 better define the functional roles of alphaB-crystallin, we generated loss-of-function zebrafish muta

296 The chaperone crystallin, which is a mild modulator of the lag phase o

297 This holds in particular for human alphaB-crystallin, which is strongly crowded in vivo and inter

298 vely monomeric human eye lens protein gammad-crystallin, whose aggregation leads to cataract disease.

299 mplexes of human alphaA-crystallin or alphaB-crystallin with alcohol dehydrogenase or citrate synthas

300 Crystallins with Greek keys motifs have significantly hi