ライフサイエンスコーパス: culture

1 13.8% of enrolled inpatients with a positive culture).

2 tocyte quantity and quality in P. falciparum culture.

3 efficient drug liberation in plasma and cell culture.

4 terial targets compared to routine bacterial culture.

5 mples was tested for Borrelia burgdorferi by culture.

6 O differentiation that declines over time in culture.

7 igodendrocytes, and can form neurospheres in culture.

8 o not compromise replicative fitness in cell culture.

9 profiling of small exRNAs isolated from cell culture.

10 ectrical and synaptic activity of neurons in culture.

11 ormed virus-like particles when expressed in culture.

12 ic constructs of the evolutionary origins of culture.

13 th of both F. graminearum and P. pastoris in culture.

14 outcome of time to positivity (TTP) of blood culture.

15 ission of models of cumulative technological culture.

16 (BDNF)-induced pTRKB in cortical neurons in culture.

17 hic and cultural associations to the Yamnaya culture.

18 low BATF binding in both mouse and human Th9 cultures.

19 EGFR-TKIs show enhanced efficacy in spheroid cultures.

20 mon features of lullabies found in different cultures.

21 o classical two-dimensional endothelial cell cultures.

22 throid cell line and primary human erythroid cultures.

23 n ectopically expressed in tobacco BY-2 cell cultures.

24 tro osteoclastogenesis in murine bone marrow cultures.

25 n hepatocytes formed spheroids in suspension cultures.

26 mor cells in different in vitro 2D and 3D co-cultures.

27 ion is often preceded by inappropriate urine culturing.

28 resent Candida growth in all 3 sets of blood cultures (15.4% vs 45.1%; P = .005) and had less severe

29 Among these positive cultures, 49 (53.3%), 29 (31.5%), and 13 (14.1%) were co

30 ategories can facilitate the transmission of culture-a feature that songbirds and humans share.

31 The addition of IL22 to cultured acinar cells increased their expression of mark

32 spanning several cells, are abundant both in cultured aggregates and in MPM surgical specimens.

33 ed Il25, Il33, and muc5ac mRNA expression in cultured airway epithelial cells.

34 Prolonged culture also revealed that purified subpopulations of MD

35 to accommodate the growing body size during culture and emulate the body gait and locomotion of anim

36 can be used for applications (such as T-cell culture and expansion) requiring high-avidity molecular

37 ureteric buds branched in three-dimensional culture and expressed Hoxb7, a transcription factor that

38 ires basic molecular biology, mammalian cell culture and fluorescence microscopy skills.

39 tide-decorated nanoparticles was shown in 2D culture and further demonstrated in spheroids.

40 Cytokine concentrations in supernatants of culture and in cell extracts were measured using Illumin

41 e function of a broad range of stem cells in culture and in tissue.

42 hese effects are rapidly compensated both in culture and in vivo by lipidome-wide remodeling, most no

43 e Kunjin virus helicase on infection in cell culture and in vivo This work provides new insight into

44 promote toxicity against mammalian cells in culture and lethality during mouse bacteremia.

45 examined by using human keratinocyte tissue culture and mouse models.

46 le strategies for the fabrication, perfusion culture and volumetric analysis of large tissue-like con

47 xpression and reduced growth of HCC cells in culture and xenografts of HCC tumors, suggesting that in

48 The EGR 1 KO cells were then cultured and stimulated with VEGF A and FGF 2.

49 resistant Tau aggregates extracted from cell cultures and human AD brain tissues, demonstrating the a

50 The sensor is applied to neuron-glial cultures and macrophage under the stimulation of lipopol

51 We used rat hippocampal cultures and their acute cholesterol depletion by methyl

52 substrate is proposed for in-situ HeLa cell culturing and real-time detection of the released H(2)O(

53 he complex relationship between European UP "cultures" and past population dynamics, they leave open

54 . gingivalis (ATCC 33277) was grown in broth culture, and lipids were extracted and fractionated by h

55 richia coli, mouse embryonic fibroblast cell cultures, and Arabidopsis thaliana leaves.

56 entrations (5-40 ug/ml) were added to neuron cultures, and cells were plated either onto well plates

57 y to confocal microscopy in primary cortical cultures, and from acute hippocampal and cortical slices

58 abarcoding analyses with mid/high-throughput culturing approaches.

59 -specific sensitivity and specificity, using culture as a reference, were similar with the Xpert-only

60 ative monolayer over the shaped scaffold and cultured as stem/proliferative cells to expand them and

61 We recently developed an organotypic slice culture assay for sensitive detection of scrapie prions

62 When E681 was cultured at 20 degrees C or lower, it exhibited no varia

63 When cultured at air-liquid interface, it was possible to fol

64 xposure and the results of the gold-standard culture-based AST measured over days.

65 ores to complement the conventional lengthy, culture-based surface sterility validation, which is cri

66 Using culture-based testing as the standard, the negative pred

67 es adjusted for age, sex, >=3 positive blood culture bottles, native valve disease, prosthetic valve,

68 ously, we analyzed protein turnover rates in cultured brain cells under basal neuronal activity and f

69 oning is crucial to cumulative technological culture but it fails to discuss the implications for the

70 C3aR1 responses were only present in primary cultures but not in situ, suggesting that the expression

71 hours to complete, excluding the time spent culturing C. elegans, and includes (i) experimental desi

72 in- and loss-of-function studies of HDAC7 in cultured cardiomyocytes implicated HDAC7 as a prohypertr

73 Cultured cell lines are widely used for research in the

74 e novel missense NOTCH1 and DLL4 variants in cultured cells demonstrate reduced signalling activity,

75 hat conclusions based on studies of Doc2b in cultured cells do not necessarily generalize to mature s

76 D), are inhibited in c9ALS/FTD brains and in cultured cells expressing either of two arginine-rich di

77 cit insulin-like signaling by mutant INSR in cultured cells, but whether this translates into meaning

78 elta39) exhibited decreased viral fitness in cultured cells, suggesting the feasibility of using this

79 e lipolytic effect of GH in humans, mice and cultured cells.

80 on of neuronal culture networks and neuronal culture cluster networks by estimating the importance of

81 imental effects of Fe(0) and bioaugmentation cultures coinjection for in situ treatment of chlorinate

82 el and mycolytic/fungal (myco/f lytic) blood culture collected simultaneously during hospitalizations

83 the laboratory-adapted strain American Type Culture Collection 17978.

84 ly used to extrapolate from the nominal cell culture concentration to PCB tissue levels and vice vers

85 ibrosis in Grp78 KO mouse and IPF lung slice cultures.Conclusions: These results support a causal rol

86 We observe that standard culture conditions are associated with a rapid and selec

87 degradation of WGA-labeled GCX under UF cell culture conditions or in Balb/C mice and led to an over

88 characteristics relative to those of static culture conditions under both baseline conditions and wi

89 analyze MS datasets from various strains and culture conditions.

90 leatum viability under standard aerobic cell culture conditions.

91 ancer-related genes and implicated selective culture conditions.

92 os, and in both primed and naive pluripotent culture conditions.

93 Our statistical model estimates that culture-confirmed cases of typhoid fever lead to an exce

94 ver information for 1029 patients with blood culture-confirmed enteric fever or with a nontraumatic t

95 ree buccal swab samples from 123 adults with culture-confirmed TB in Lima, Peru.

96 patients > 18 years receiving treatment for culture-confirmed, drug-sensitive TB from 2000 to 2016 a

97 Our data shows that tumor-stroma co-cultures consisting of aligned extracellular matrix (ECM

98 lus Amoebocyte lysate assay in real bacteria culture containing naturally occurring LPS, with similar

99 Culture contamination remained more frequent in the cont

100 udy documents the end-of-treatment outcomes, culture-conversion rates, and serious adverse events (SA

101 In cultured coronary arterial smooth muscle cells (CASMCs)

102 cesses essential to cumulative technological culture (CTC).

103 ely diluted intact bacterial cells from pure cultures, culture-spiked cattle feces, and culture-spike

104 The major dechlorinating species in the seed culture, Dehalococcoides, were not responsible for the d

105 Conversely, the monolayer differentiation culture derived a mixture of Tbr1 and Ctip2 mature neuro

106 we investigated, in rat hippocampal neuronal cultures derived from embryos of unknown sex, whether BD

107 conditions to produce intracellular HEV cell culture-derived particles (HEVcc) with viral titers betw

108 l hospitalized patients who received a blood culture during hospitalization.

109 These cultures effectively recreate many of the physiological

110 n contrast to the general belief that modern culture evolves very quickly, we show that rates of mode

111 Blood cultures experimentally inoculated with Enterobacteriace

112 ed claudin-8 expression, confirming the cell culture experiments' findings.

113 With variations among single and binary cultures, fermented-AP counteracted the inflammatory pro

114 ication in mice, it was not observed in cell culture, ferrets or human challenge participants.

115 itional 138 inpatients (0.4%) had a positive culture for typhoidal Salmonella.

116 res for S. Typhi and 297 (1.4%) had positive cultures for S. Paratyphi A.

117 nts, of whom 2116 (10.1%) had positive blood cultures for S. Typhi and 297 (1.4%) had positive cultur

118 Viral culture from respiratory samples was positive for 19 of

119 ma virus matched replication-competent virus cultured from CD4+ T cells.

120 D1 on LTB4-induced responses of goblet cells cultured from rat conjunctiva.

121 s attenuated in weakly IB4+ and IB4- neurons cultured from rats whose OIHP was reversed in vivo Thus,

122 d antibiotic resistance results of bacterial cultures from hospitalized patients, alongside their ele

123 can explain the activity against planktonic cultures, garlic has no activity against biofilms.

124 ion, we also profiled the heterogeneous cell cultures generated from multiple human pluripotent stem

125 Grafts with all cells cultured >=96 hours did not contain more beta cells but

126 Although Treg stability during ex vivo culture has improved, methods to assess Treg stability s

127 spects on the safety assessment of microbial cultures have been suggested, no methodological detail n

128 o effectively inhibited NAPE-PLD activity in cultured HEK293 cells.

129 and cellular stress-gated switch function in cultured hippocampal neurons.

130 e-stop microfluidic platform to assemble and culture human cerebral organoids from human embryonic st

131 Treatment of cultured human adipocytes with troglitazone increased PP

132 P4 was more active in supporting adhesion of cultured human and mouse macrophages in experiments usin

133 ACRG also affected these pathways in various cultured human cell lines and in mouse embryonic fibrobl

134 Addition of purified LOXCAT to the medium of cultured human cells with a defective electron transport

135 ression by ascorbic acid was accomplished in cultured human keratinocyte stem cells to show similar C

136 Here we describe an ex vivo cultured human skin explant model in which we have chara

137 ciliate infestation of a larval geoduck clam culture in a commercial hatchery to investigate the mole

138 Akt and p90-RSK pathways specifically in ULA culture in ILC cells.

139 acental villous cytotrophoblasts followed by culture in TSC medium to maintain cellular proliferation

140 ramatically increased in TNFR2pos Treg after culture in vitro with S. aureus.

141 and primiR-199a2 mRNA levels in mouse islets cultured in 10 mm glucose compared with 5.5 mm glucose.

142 GBM cells and patient-derived GBM cells cultured in 3D microwells were co-treated with BAY 11-70

143 histology processing for the entire samples cultured in a multi-well plate.

144 the cellular level, Opn3-KO brown adipocytes cultured in darkness had decreased glucose uptake and lo

145 Our studies on several meningioma cell lines cultured in hypoxic conditions validated the association

146 Human subcutaneous fat was cultured in vitro to promote blood vessel outgrowth prio

147 CRISPR-Cas technology in granulosa cells and cultured in vitro with BMP-4 stimulation for three diffe

148 om those isolated from blood and bone marrow cultures in southern India, over 26 years (1991-2016).

149 across a variety of different countries and cultures in two of the most commonly used designs in exp

150 has challenged the primary role assigned to culture, in determining this spatial numerical associati

151 tially enriched in DCB versus TCE enrichment cultures, indicating that they may play a role in dechlo

152 We also demonstrate that this co-culture-induced chemoresistance is abrogated by inhibiti

153 overwhelmed the capacity of automated blood culture instruments.

154 Blood culture is not widely available in endemic settings and,

155 The creation of edited plants through tissue culture is often inefficient, time-consuming, works for

156 n of over 10,000 bacterial genomes from both cultured isolates and metagenomic datasets, revealing th

157 Using 3D cell culture, it is shown that drug release is commensurate w

158 Using a cultured keratinocyte model system, we show that depleti

159 ogenesis of the DUB mutant virus (DUBmut) in cultured macrophages and in mice.

160 Early cultures maintain the clonal heterogeneity seen in PDAC

161 Despite the difficulty in culturing many microbes from an environment, we can stil

162 Standard microbiological culture may fail to identify unusual or fastidious organ

163 In cultured MDCK cells, CRB2S associates with apical membra

164 Using the culture media of human embryos with normal morphologic f

165 d potential mispaired side products, in cell culture media, or other complex matrices.

166 PCBs were present in the cell culture medium (60.7-88.8%), cells (8.0-14.6%), and dish

167 and placed in a well plate containing organ culture medium 1 without dextran.

168 SP and IL-1beta levels were assayed in the culture medium by ELISA.

169 We elucidate the significance of this new culture medium for chronic disease modeling of IL-13-ind

170 ly, we generate a minimal chemically-defined culture medium in which IGF1 together with Activin maint

171 Pure L. murinus conditioned culture medium inhibited growth and reduced the extensio

172 ineage was significantly altered by both the culture method [air-liquid interface (ALI) vs submerged]

173 In conclusion, this robust cell culture model of HEV infection provides a powerful tool

174 Finally, overexpression of ROBO2 in cultured mouse podocytes compromises cell adhesion.

175 For culture-negative samples, mean cost-per-positive 16S PCR

176 four cases were culture-positive and 26 were culture-negative.

177 gate the structure and evolution of neuronal culture networks and neuronal culture cluster networks b

178 cological elevation of membrane PI(4,5)P2 in cultured neurons impairs SV endocytosis, specifically in

179 Ps had no effect on synaptic connectivity in cultured neurons or in vivo, but impaired NMDA-receptor-

180 atic prion-infected mice are highly toxic to cultured neurons, exceptionally pure intact high-titer i

181 Exposure of cultured NPCs to hypoxia and sovateltide also showed hig

182 tories and is designed to cultivate a campus culture of ethical science and engineering research in t

183 This platform could support long-term culture of intestinal organoids, potentially replacing t

184 In contrast, co-culture of LVS-infected macrophages with LVS-immune lymp

185 Culture of MAIT cell supernatants with B cells led to gr

186 growth dependent on Mn(II) oxidation to a co-culture of two microbial species.

187 Efm isolates from gastrointestinal and blood cultures of 24 pediatric patients undergoing chemotherap

188 lar delivery was achieved in all cells in 2D cultures of a human bladder epithelial progenitor cell l

189 radation of trifluralin does occur, and pure cultures of bacteria and fungi capable of partially degr

190 of a gp41 CT truncation mutant to spread in cultures of MT-4 cells.

191 ents with murine xenografts and 2D and 3D co-cultures of NHFs and PDAC cells revealed that older NHFs

192 When primary cultures of Pam (0-Cre-cKO/cKO) atrial myocytes (no Cre

193 In cultures of striatum or ventral midbrain, CHL1 was also

194 The absence of prophages in cultures of the dominant lineages of marine bacteria has

195 y, we demonstrate effects of ISEMF and EC co-culture on patient-derived human intestinal epithelial c

196 When cells were cultured on a topography that mimics the epidermal-derma

197 d osteoclasts, enhanced bone resorption when cultured on bone slices, and altered mRNA expression of

198 Keratocytes cultured on stiff (10 kPa) gels or collagen-coated glass

199 ondrogenic differentiation compared to BMSCs cultured on the assemblies with larger pore size.

200 0 [96-98%]) among participants with negative cultures on raw sputa.

201 Osteomyelitis was confirmed by bone culture or histopathology.

202 ologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or bl

203 ches for high-level Taxol production in cell cultures or microbial hosts.

204 The argument that cumulative technological culture originates in technical-reasoning skills is not

205 In this study, we cultured ovarian cancer cell lines in adherent and nonad

206 MP significantly enhanced Ryr2 expression in cultured photoreceptor-derived Weri-Rb1 cells.

207 exin 43 (CX43) gap junction communication in cultured pluripotent cells from human dental follicles (

208 Twenty-four cases were culture-positive and 26 were culture-negative.

209 Change in clinical management for culture-positive eyes was based on declining vision (3 e

210 19 HIV-seronegative inpatients, 46 (39%) had culture-positive pulmonary TB.

211 ter cataract surgery, of which 57 (51%) were culture-positive.

212 ts with drug-resistant TB were cough aerosol culture-positive.

213 s well as sputum-smear microscopy and sputum-culture positivity at 2 and 6 months.

214 Culture positivity at day 3 conferred a hazard ratio of

215 Blood culture positivity results for true pathogens and contam

216 cin) had 1.32-fold increased odds of 24-hour culture positivity, although this was not statistically

217 ve treatment largely abrogated cough aerosol culture positivity; however, this was not always rapid.

218 n the tissue bulk severely limits the tissue culturing potential of many bioreactors.

219 Positive bacterial respiratory culture predicted pulmonary dysfunction at discharge (od

220 By using bacterial cultures present in the surroundings or raw materials, d

221 In cultured primary astrocytes stimulated with lipopolysacc

222 rent genomic break clusters, termed RDCs, in cultured primary mouse neural stem and progenitor cells

223 r Ca(2+) and neurotoxicity in Drosophila and cultured primary mouse neurons.

224 Using host cell-free culture, progesterone was determined to have a direct in

225 for cryptococcal disease with LPs and blood cultures, prompt ART initiation, and more intensive anti

226 Culture-proven aspiration pneumonia and early-onset vent

227 Five of 6 cases (83%) had culture-proven infectious endophthalmitis (2 Candida gla

228 to dendritic spines and synaptic deficits in cultured rat hippocampal neurons.

229 Nursing homes have highly divergent urine culturing rates; this variability is associated with hig

230 In organotypic slice cultures, repeated NPY treatment reduces the complexity

231 More than 70% of the UHGG species lack cultured representatives, and 40% of the UHGP lack funct

232 Annexin A5 addition to choroid plexus cell cultures restored the Abeta-induced impairments on autop

233 ce of select clinical features against blood culture results among outpatients using mixed-effect reg

234 r pressure (IOP), clinical presentation, eye culture results, and treatments performed.

235 5%) PVC results and 173 (75%) negative valve culture results.

236 fermenting, gram-negative bacteria in blood culture(s) if they were afebrile for 24 hours without ev

237 directional PCR/sequencing of residual blood culture samples.

238 nd viral culture; the limit of detection for culturing SARS-CoV-2 from surfaces was determined.

239 1 diabetic (T1DM) mouse models together with cultured Schwann cells (SCs) and skin biopsies from pati

240 regulated LTRs into recipient human cells in culture showed robust and heterogenous activation of six

241 tions and functional assays of cSCC cells in culture showed that LINC00346 expression is down-regulat

242 On the other hand, bioaugmented culture showed the lowest caproate production in the sec

243 hanogenic cultures, whereas nitrate-reducing cultures showed no SMX transformation.

244 Pulsed Stable Isotope Labeling in Cell culture (SILAC) approaches allow measurement of protein

245 anels directly applicable to blood and blood culture specimens, next-generation metagenomics, and gas

246 d intact bacterial cells from pure cultures, culture-spiked cattle feces, and culture-spiked ground b

247 e cultures, culture-spiked cattle feces, and culture-spiked ground beef.

248 We also determined that breast cancer co-culture stimulated lymphangiogenic signaling in LECs, ye

249 Human cultures store memories in large distributed assemblies

250 n mice, animal behavioural analysis and cell culture studies to identify a highly conserved regulator

251 ors with acute HIV-1 infection and one viral culture supernatant were serially diluted into 25-ml sam

252 onitors the protein size and quantity in the culture supernatant.

253 identified the major proteins present in the culture supernatant.

254 The culture system affords 236- to 899-fold expansion over t

255 nstrate that click-ExM is applicable in cell culture systems and for tissue imaging.

256 of ZNF410 in adult-type human erythroid cell culture systems and xenotransplantation settings diminis

257 Three-dimensional (3D) culture systems have fueled hopes to bring about the nex

258 er redox regulatory genes in both 2D- and 3D-culture systems, uncovering a vulnerability of spheroid

259 acerbated by limitations of traditional cell culture techniques, which fail to recapitulate mechanobi

260 Here we refine an enrichment culture that exhibits exponential growth dependent on Mn

261 shed to campaign for reforms of the research culture that has led to shortcomings in rigor.

262 enteroids (HIEs) are primary epithelial cell cultures that can provide insights into the biology of t

263 d subpopulations giving rise to heterogenous cultures that represented the entire hierarchy of MDSC p

264 s due in part to an inability to efficiently culture the virus in vitro for neutralization assays.

265 For Gram-negative cultures, the Verigene result correlated with unnecessar

266 SARS-CoV-2 was detected by RT-qPCR and viral culture; the limit of detection for culturing SARS-CoV-2

267 ons detach from the chip after a few days of culture; this process is mediated by the upregulation of

268 ls can be magneto-patterned in hydrogels and cultured to generate heterogeneous tissues.

269 Following IVF embryos were cultured to the blastocyst stage in vitro or transferred

270 the remarkable self-assembly capacity of 3D cultures to form functional circuits that could be used

271 s highlight the importance of considering 3D cultures to model host-pathogen interaction.IMPORTANCE T

272 ulation of T cells in ex vivo vaginal tissue cultures triggered antiviral responses in myeloid and CD

273 Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells +

274 of motile cell populations, both primary and cultured, typically reveals an intercellular variability

275 HGPS-iPSC SMCs cultured under arterial flow conditions detach from the

276 hat does not meet the sufficient dose can be cultured until this is reached after combination with su

277 rate GBOs and 5-7 d to perform CAR T cell co-culture using this protocol.

278 were collected from the headspace above the cultures using solid phase microextraction (SPME) and we

279 March 2020 led to a sharp increase in blood culture utilization, which overwhelmed the capacity of a

280 , AST within 7 days of initial positive MSSA culture was associated with survival.

281 Under this umbrella definition, culture was for many decades the exclusive province of t

282 r 2018, from patients >=18 years of age, and culture was performed as part of standard-of-care testin

283 ified adult retinal ganglion cells (RGCs) in culture, we demonstrated here that VEGF is released by R

284 osed by microscopy, PCR-based detections and culture: we showed M. smithii microscopically and by a s

285 interface on a stainless-steel grid in cell culture wells containing a nutrient solution.

286 od was created; patients with positive urine cultures were excluded.

287 OvC-PDE cultures were exposed to standard-of-care chemotherapeut

288 aired T2Candida panel and myco/f lytic blood cultures were identified.

289 The tissue cultures were incubated at 37 degrees C in a 5% CO(2) en

290 Cell lines and acini cultures were incubated with IL22 and pharmacologic inhi

291 ateral eye and their outgrowths after 2-week cultures were transplanted on the affected eye after pan

292 should use this panel as an adjunct to blood cultures when making a definitive diagnosis of candidemi

293 assays with SH-SY5Y human neuroblastoma cell cultures, where Abeta42 aggregation into large insoluble

294 ed in both sulfate-reducing and methanogenic cultures, whereas nitrate-reducing cultures showed no SM

295 male mice maintained migratory capacity when cultured with FFA, whereas female macrophages failed to

296 e was reproduced in vitro when cells were co-cultured with IL4-polarized MPhi.

297 Mouse embryonic stem cells (mESCs) cultured with MEK/ERK and GSK3beta (2i) inhibitors trans

298 Treatment of CS-exposed ALI cultures with interferon beta-1 abrogated the viral infe

299 ace, facilitating infection of AT2 and basal cultures with SARS-CoV-2 and identifying club cells as a

300 fabifermentans ALI6 used singly or as binary cultures with the selected Saccharomyces cerevisiae AYI7