ライフサイエンスコーパス: culture method

1 ensional (3D) Madin-Darby canine kidney cell culture method.

2 im broth was compared to the CDC-recommended culture method.

3 limited if keratinocytes get altered by the culture method.

4 ly diverse subtype population than the solid culture method.

5 ty and 98.1% specificity versus those of the culture method.

6 n comparison to that of the semiquantitative culture method.

7 xcellent concordance with a more established culture method.

8 antigenemia assay and the conventional tube culture method.

9 as equivalent in sensitivity to our standard culture method.

10 well-characterised and functionally relevant culture method.

11 inguished from non-pathogenic strains by the culture method.

12 th culture using a standard automated liquid culture method.

13 h is much faster than the conventional viral culture method.

14 ar to that of the hornworm by patterned cell culture method.

15 NoVs remains lacking due to a lack of a cell culture method.

16 30) of which were also negative based on the culture method.

17 xamined for NTM using a membrane filtration, culture method.

18 the results of standard smear microscopy and culture methods.

19 ing on-site for Campylobacter, 97% used only culture methods.

20 method, a combination of direct and enriched culture methods.

21 (54.7% [95% CI, 42.7%-66.2%]) compared with culture methods.

22 re assessed with respect to case finding and culture methods.

23 time of MTBC identification of gold standard culture methods.

24 colonization was determined by conventional culture methods.

25 incidence was reported when using automated culture methods.

26 bacterial pathogens that are undetectable by culture methods.

27 ganisms that remain undetectable by standard culture methods.

28 because it cannot be recovered using routine culture methods.

29 ine utilization of the relatively slow viral culture methods.

30 arized scaffolds, a co-culture, and a sphere culture methods.

31 numbers by standard clinical microbiological culture methods.

32 ecially difficult to identify using standard culture methods.

33 A and BDGO assays when they were compared to culture methods.

34 l epithelial cells cultured with organotypic culture methods.

35 lid fecal culture or automated liquid medium culture methods.

36 olonization by lactobacilli were assessed by culture methods.

37 cellular products, remains essential to the culture methods.

38 Tissue banks should validate processes and culture methods.

39 direct plating or enrichment broth selective culture methods.

40 and susceptibility testing with traditional culture methods.

41 Each patient was screened by 32 culture methods.

42 it (2-5% of the microbiota) of non-selective culture methods.

43 tudies using conventional in vitro bacterial culture methods.

44 ith pulmonary tuberculosis than conventional culture methods.

45 are unrecognized by conventional laboratory culture methods.

46 = MM-3) cell lines were grown using standard culture methods.

47 enhanced recovery characteristics of fungal culture methods.

48 an be detected by either autofluorescence or culture methods.

49 ial diversity that is unattainable with most culture methods.

50 roducts such as meat through scalable tissue culture methods.

51 85 (52.1%) patients by a combination of all culture methods.

52 sing flow cytometry and cell differentiation culture methods.

53 the highest sensitivity [64% (35-87)] among culture methods.

54 ses of Salmonella spp. were identified using culture methods.

55 ng immunohistochemistry and 3D in-vitro cell culture methods.

56 t our laboratory's contribution to promoting culture methods.

57 an mucosa for microbiological screening with culture methods.

58 is also cost-saving compared to conventional culture methods.

59 ked by conventional acid-fast bacillus (AFB) culture methods.

60 rched for such factors using newly developed culture methods.

61 versatile and compares well to C. difficile culture methods.

62 aginal swabs were used for the wet mount and culture methods.

63 ier using the BC-GP test compared to routine culture methods.

64 interactions, overcoming the limitations of culturing methods.

65 f the limits of current metagenomic and cell culturing methods.

66 that HPIV-1 may be underdiagnosed by routine culturing methods.

67 or Shigella entero-pathogens in traditional culturing methods.

68 ed RNA virus inactivation without relying on culturing methods.

69 BIA-ALCL patients (n = 7), and controls via culturing methods, 16S rRNA microbiome sequencing, and i

70 Of 3,923 samples tested by both DFA and culture methods, 22 (3 by DFA testing and 21 by culture)

71 Of 1,426 samples tested by both DFA and culture methods, 48 (44 by DFA testing and 15 by culture

72 detected more true-positive results than the culture method (58 versus 55 swabs); however, this diffe

73 of 16s rRNA sequencing replacing traditional culture methods, a strong association between the presen

74 Compared to passive screening using culture methods, active screening resulted in discontinu

75 ed from investigations utilizing traditional culturing methods, advances in sequencing technologies h

76 ly induced DNA damage in the nematode or how culturing methods affect DNA damage levels.

77 ineage was significantly altered by both the culture method [air-liquid interface (ALI) vs submerged]

78 nient tool that can be embedded into routine culture methods, allowing the culture of all sputum samp

79 This two-step culture method allows for the production of large number

80 was significantly more sensitive than either culture method alone and was also more sensitive than th

81 Primary tissue culture methods also are described, and their value in u

82 ystem compared favorably to the CDC enriched-culture method and to the BD MAX GBS assay.

83 ct these growth components by comparing cell culture methods and identifying the benefits and pitfall

84 ul additions to traditional preclinical cell culture methods and in vivo animal studies in the near t

85 er, conventional tests including traditional culture methods and nucleic acid amplification tests hav

86 This approach is compared with conventional culture methods and PCR-based assay, as well as with imm

87 easured before and during gull control using culture methods and quantitative polymerase chain reacti

88 of the biopsy specimens was performed using culture methods and real-time polymerase chain reaction

89 etection sensitivity than conventional urine culture methods and resulted in typical colony growth at

90 tanding microalgal heterogeneity, optimizing culture methods and screening mutant microalgae.

91 the first attempts were made to develop mass culture methods and to formulate artificial diets that w

92 Diverse culturing methods and detection of various combinations

93 .5-100% accuracy, which were superior to the culture method, and comparable to PCR but without requir

94 to study MSCI ex vivo, based on a short-term culture method, and demonstrate that active DDR signalin

95 specimen collection in pregnancy, selective culture methods, and study sample size did not explain t

96 lt, and potentially greater sensitivity than culture methods, and they should be considered the new g

97 relationships within ecosystems, develop new culturing methods, and discover new products and process

98 Furthermore, the liquid culture method appeared to select for a more genotypical

99 Sputum induction was offered, and different culture methods applied to a subset of Cohort B tongue s

100 l Africa was investigated with molecular and culture methods applied to preserved core samples.

101 n of antecedent antimicrobial therapy as SMT culture methods are dependent on the recovery of viable

102 Culture methods are not useful in characterisation of po

103 Standard culture methods are preferred for hospital diagnostic mi

104 Reliable, scalable and time-efficient culture methods are required to fully realize the clinic

105 in the United States, and traditional organ culture methods are still used in Europe.

106 Enriched culture methods are the current standard for prenatal GB

107 Conventional culture methods are time-consuming and associated with a

108 However, culture methods are time-consuming and need at least 24

109 Costly, genotype-dependent tissue culture methods are used in many crops, while seed-based

110 Three-dimensional cell culture methods are viable in vitro approaches that faci

111 ical utility of rapid detection (vs standard culture methods) are scant.

112 rrent diagnostic methods, heavily reliant on culture methods, are slow and often inefficient.

113 nt the often-inadequate yield of traditional culture methods as a substantial percentage of orthopedi

114 ble to those of standard and/or single rapid-culture methods as shown by parallel testing of 590 fres

115 nscription-PCR and was detected later by the culture method at the time of moribund necropsy.

116 the MS5 coculture system or a novel defined culture method based on pharmacological inhibition of bo

117 owth in vitro will not only allow for better culture methods but also advance the field towards provi

118 o this end, we compared two established CEnC culture methods by assessing the transcriptomic changes

119 This clonal culture method can be scaled up to produce NSCs for diff

120 Whole-culture methods cannot produce a set of cells that refle

121 There is a need for cell culture methods capable of dissecting the intricate regu

122 cterial pathogen recovery using conventional culture methods (CCMs), while PCR-based diagnostics are

123 The modified culture method could be utilized to improve screening fo

124 This vOrganoid culture method could not only serve as a model to study

125 rom the same fecal samples suggests that the culture method could provide a "microbiological" bias an

126 The isolation and co-culture methods could provide a stable platform for crea

127 ectant, but the conventional microbiological culture methods currently used target only a very small

128 While the culture method detected 10(4) CFU/g in ground pork and 1

129 Overall, the standard culture methods detected Shigella spp., EPEC, ETEC, and

130 Using tissue culture methods developed to study high-risk HPV types,

131 he tumor microenvironment; however, standard culture methods did not yield readily cultivable microbi

132 sure drugs, whereas conventional bone marrow culture methods do not.

133 ouse gene-targeting protocol include ES cell culture methods, drug selection scheme, colony picking a

134 Bacterial culture methods, e.g. Plate Counting Method (PCM), are a

135 , we introduce the extended endothelial cell culture method (EECM), which differentiates hiPSC-derive

136 This may partly be due to the culturing methods employed in hospital laboratories, whi

137 This stable isotope labeling in cell culture method enables the unequivocal identification of

138 on of mycobacteria, but as with other liquid culture methods, ESP II should be used in combination wi

139 they were not detected by standard clinical culture methods, especially for low-prevalence or fastid

140 Our perfusion culture method extends tumor slice viability, maintainin

141 syndrome is often empirical because clinical culture methods fail to detect prostate-associated patho

142 Here, we describe an organoid culture method for cultivating patient-derived lymphoma

143 onsiderably more sensitive than the standard culture method for detecting VRE directly from perianal

144 nostic Systems, Sparks, Md.), and a standard culture method for detection of group A streptococci (GA

145 late method combined with a broth enrichment culture method for detection of group B streptococcus co

146 d a robust, reliable, three-dimensional (3D) culture method for medulloblastoma able to recapitulate

147 Previous studies describe a unique culture method for the commitment of murine embryonic st

148 CSA) and a standard acid-fast bacillus (AFB) culture method for the isolation of nontuberculous mycob

149 (BCSA) has been recommended as an expedient culture method for the isolation of rapidly growing NTM

150 known, mainly due to the lack of a long-term culture method for this parasite.

151 were evaluated by comparison to traditional culture methods for detecting Chlamydia trachomatis and

152 t the MAGI assay is more sensitive than PBMC culture methods for detecting infectious virus in female

153 arker gene-selection combinations and tissue culture methods for efficient regeneration of transplast

154 PCR-hybridization was compared to culture methods for evaluating suspected blood infection

155 essment of HIV-cure strategies using ex vivo culture methods for further understanding of the diversi

156 results demonstrate the utility of rapid DC culture methods for high efficiency in vitro T cell sens

157 aches since the isolation and development of culture methods for human pluripotent stem cells, as wel

158 This paper establishes culture methods for immature sheep cardiomyocytes and re

159 We sought to develop culture methods for immature sheep cardiomyocytes in ord

160 remains difficult to study in the absence of culture methods for luxuriant growth.

161 With the recent FDA approval of culture methods for platelet bacterial testing and the p

162 WGS is a promising alternative to culture methods for resistance prediction in S. aureus a

163 Studies have demonstrated that large-volume culture methods for sterile body fluids other than blood

164 nsitivity as an exemplar to (i) develop cell culture methods for the detection of drug-specific B-cel

165 However, most 3D culturing methods generate either brain or retinal organ

166 stem cells (BMSCs) using the in vitro direct culture method, greater cell adhesion densities were obs

167 Current cell culture methods, however, cannot cost-effectively produc

168 A recently developed culture method identified glial cell line-derived neurot

169 specificity (96.7%) compared to those of the culture method in this multisite evaluation.

170 combined direct and broth-enriched toxigenic culture methods in a large, multicenter clinical trial.

171 and displayed similar diagnostic accuracy as culture methods in children with suspected tuberculosis.

172 re as sensitive and specific as conventional culture methods in detecting Salmonella spp. from feces.

173 ues during the development of general tissue culture methods in the 1950s to 1970s or from specimens

174 een numerous proposals suggesting that whole-culture methods - in which all cells in a growing cultur

175 lymerase chain reaction (PCR)-based tests to culture methods, in patients with postoperative endophth

176 The culture method included direct inoculation of a MacConke

177 However, most culture methods involve the use of animal serum to estab

178 and further validation of the proposed novel culture method is required.

179 A major challenge in the current culturing methods is the lack of accurately capturing mu

180 ell purification techniques and quantitative culture methods, it was found that IFN-alpha directly in

181 Culture methods lack the capacity to detect VBNC cells,

182 o study due to the lack of a robust in vitro culture method, low parasite densities in peripheral cir

183 Improvements in stem cell culture methods, materials and biophysical tools that as

184 Current culture methods may fail to detect non-O157 STEC.

185 ignals from relatively rare cells while cell culture methods may significantly alter cellular phenoty

186 remia (57 versus 37%; P = 0.004), making any culture method more likely to identify them.

187 Plant tissue culture methods need optimization and simplification for

188 owever, newly developed 3D in vitro organoid culture methods now allow the routine culture of primary

189 We have developed a purification and cell culture method of BFCN in order to examine the regulatio

190 Here, we develop a simple and stable culture method of mouse glial cells, termed mixed-glial

191 ome this limitation, we developed an ex vivo culture method of the mammary gland where the direct act

192 ular panel to routine quantitative bacterial culture methods on remnant BALF.

193 tobacco (Nicotiana tabacum L.) and in vitro culture methods optimised for their maturation to fully

194 significant difference (P > 0.1) between the culture methods or the ciprofloxacin concentrations in t

195 bility to be detected by standard laboratory culturing methods, or a lack of adherence and hence rapi

196 l (p < 0.001 and p = 0.012, respectively for culture methods; p = 0.012 and p = 0.034, respectively f

197 tent stem cells (hPSCs)-based 2D and 3D cell culture methods, particularly advancements in brain orga

198 This time-efficient and simplified culture method paves the way for large-scale, high-throu

199 New in vitro cell culture methods permitted isolated mouse islet progenito

200 However, traditional culture methods present a potential delay in treatment d

201 hese species remain recalcitrant to standard culture methods, prohibiting their use as sources of uni

202 Present-day islet culture methods provide short-term maintenance of cell v

203 This culture method provides a platform for studying the BCR

204 The described culture method provides quantitation of the cell produci

205 Typical sputum culture methods quantitate the relative amounts of each

206 l depend on enhancing the sensitivity of the culture method rather than increasing the volume of mate

207 S LB assays, to that of the standard of care culture method recommended for GBS screening using 500 v

208 g the combined direct and enriched toxigenic culture method (reference method), for a prevalence rate

209 When the PCR-hybridization and culture method results were compared, the positive and n

210 assessed by the IB method and the reference culture method (RM).

211 rs at five US cystic fibrosis centres, using culture methods sensitive for small-colony variants.

212 carried out days or even weeks before other culture methods, significantly reducing associated labor

213 nd genome-engineered therapies, yet existing culture methods still allow substantial HSC loss.

214 Recently, 3D cell culture methods, such as stem cell-based spheroids and o

215 The current gold standard based on culture methods takes up to 10 days to show positive res

216 We have developed a microfluidic cell culture method that allows for the formation of linear i

217 a simple, scalable, and repeatable organoid culture method that can be used not only for human brain

218 of Cell Stem Cell, Csaszar et al. develop a culture method that overcomes current limitations in ex

219 ity, and mortality emphasizes the need for a culture method that permits simultaneous isolation and d

220 In this study, we describe a novel culture method that uses human brain endothelial cells (

221 We describe here primary culture methods that allow C. elegans embryonic cells to

222 rally involves empirically determined tissue culture methods that are based on the principle of induc

223 plication of hESC-derived cells will require culture methods that are low-cost, robust, scalable and

224 ial, however, depends on the availability of culture methods that are robust, scalable, and use chemi

225 developing both specific assays and new cell culture methods that enabled them to report, in the acco

226 e successfully analyzed by MALDI-TOF MS when culture methods that suppress pigment expression are use

227 s (49%) or from frozen feces (40%) than from culture methods that used HEp-2 adherence assays (13%) (

228 Using our recently established culturing method that allows propagation of normal and c

229 We demonstrate, using two culture methods, that EMT does not underlie the appearan

230 ing, single-cell RNA sequencing and organoid culture methods, that have provided important insights i

231 ribe here a novel selective and differential culture method, the Cdifftox plate assay, which combines

232 omogenic medium was compared to the standard culture method, the sensitivity and specificity, respect

233 For direct culture methods, the agreement between MS and CA was 98.

234 f NA function because, unlike available cell culture methods, the results of such assays are predicti

235 Compared to the results of traditional culture methods, the sensitivities of a rapid chromatogr

236 mental cues that are missing in conventional culturing method, this approach did not require any gene

237 Application of this culture method to a human iPS cell line also generated r

238 We developed an in vitro culture method to characterize the expression of bacteri

239 In the present study, we use a two-step culture method to demonstrate efficient generation of fu

240 ethod which combines a wet preparation and a culture method to detect Trichomonas vaginalis.

241 Here we introduce an ex vivo feeder-free culture method to differentiate gene-engineered hematopo

242 We used a novel microfluidic single-cell culture method to directly observe the differentiation c

243 Here we develop an ex vivo co-culture method to grow cancer cells in mouse bones to as

244 rpose of this study was to use the two-layer culture method to improve donor-organ use from marginal

245 We used a high-throughput B-cell culture method to isolate neutralizing antibodies from a

246 support the development of a potential cell culture method to maintain ESR1 expression through REST

247 ic bottle may be useful as a selective blood culture method to optimize the recovery of fungi and myc

248 We used a stroma-supported culture method to study the prevalence and growth charac

249 We developed a culture method to support the efficient activation and p

250 Cell culture methods to assess CMV drug resistance in vivo wi

251 We used continuous culture methods to avoid potential confounding variables

252 diphtheria laboratory confirmation requires culture methods to determine toxigenicity, real-time PCR

253 ds, PCR could serve as an adjunct to current culture methods to facilitate early detection of bloodst

254 een impeded by the inability of conventional culture methods to interrogate microenvironments of comp

255 The facility of organoid culture methods to maintain luminal prostate epithelia,

256 Failure of current culture methods to mimic the physiologic temperature and

257 ation that researchers adopt serum-free cell culture methods to reduce animal use in this area.

258 red interest in using three-dimensional (3D) culture methods to study biology, model disease and pers

259 production of HAV antigen by standard tissue culture methods to the production of HAV antigen with th

260 le isotope labeling with amino acids in cell culture) method to monitor three conditions simultaneous

261 y metagenomic DNA sequencing and traditional culturing methods to characterize the composition and di

262 aged, highlighting the need for standardized culturing methods to improve test reliability.

263 d from 80.6%, compared to the less sensitive culture method, to 97.0%, compared to PCR.

264 one and was also more sensitive than the two culture methods used in tandem (the tandem culture sensi

265 health problem in part because the standard culture methods used to determine the appropriate treatm

266 n vivo led us to develop an efficient tissue culture method using LNSCs to generate and expand CI-T(r

267 y up to 16 weeks), (iii) liquid-solid double culture method using modified 7H9 broth (8 weeks) follow

268 immunofluorescence assay and in vitro organ culture method using NHC were used.

269 Low-cost, robust, scalable culture methods using chemically defined materials need

270 compared to those from direct and extracted culture methods using Gram staining and a GAS-specific l

271 ematopoietic stem and progenitor cell (HSPC) culture methods using polyvinyl alcohol-based media now

272 The sensitivities of culture methods using self- and clinician-collected spec

273 cant difference between the sensitivities of culture methods using self- and clinician-collected vagi

274 A culture method utilizing quantitative plating on antibio

275 A novel semisolid culture method was developed to support the formation of

276 A two-step culture method was devised to generate large numbers of

277 id antigen H (ipaH) gene by PCR and standard culture methods was used to identify Shigella species or

278 In order to improve the culture method, we investigated the effects of four cult

279 To identify the most optimal culturing method, we compared various techniques that co

280 compared to the combined direct and enriched culture method were 92.9% (131/141; 95% confidence inter

281 The sensitivities of five culture methods were determined for patients known to ha

282 y identified by conventional microbiological culture methods were identified to the species level by

283 nd Prevention guidelines that used selective culture methods were included in the analyses.

284 ficult to study because until recently blood culture methods were too insensitive to detect spirochet

285 Culture methods were used to isolate E. coli O157:H7 fro

286 Optimal clinical and culture methods were used to select 135 men with chronic

287 Here we describe a three-dimensional (3D) co-culture method whereby human muscle progenitors mixed wi

288 ol and Prevention (CDC)-recommended enriched-culture method, which served as the gold standard refere

289 lysis in ascitic fluid is currently based on culture methods, which are time-consuming and laborious.

290 identification approaches include bacterial culturing method, which takes several days.

291 film-associated infections relies heavily on culturing methods, which fail to detect nonculturable ba

292 currently available detection tools rely on culturing methods, which take more than 48 hours to dete

293 The suspended BME hydrogel culture method will allow intestinal organoids, and pote

294 We believe that the new culture method will enable inquiries into the antimicrob

295 e of this study was to compare this standard culture method with the detection of GBS directly from a

296 We compared a rigorous culture method with the Gen-Probe AccuProbe Group B Stre

297 an effectively replace the traditional batch culture methods with nanoliter volumes of bacterial cult

298 y to cancer cell signals depending on the co-culture method, with intensified transcriptome changes w

299 dividual genera, PCR was as sensitive as the culture method, with the exception of Salmonella culture

300 Here we used a single air-liquid interface culture method without modification to engineer oncogeni