ライフサイエンスコーパス: dATP

1 dATP insertion opposite M(1)dG was the most favored inse

2 dATP levels were normalized by treatment with either car

3 dATP was highly elevated in ADA-deficient cultures, and

4 sed with the dATP/ATP ratio (p < 0.01 at 10% dATP).

5 F (12a, 12b), and beta,gamma-CHCl (13a, 13b) dATP diastereomers as documented here, but the reductive

6 yl conformation (versus 3'-exo in 3'-MANT-2'-dATP).

7 near, with the nonlinearity evident at 2-20% dATP; force increased significantly with only 10% of sub

8 PAPgamma bound to cordycepin triphosphate (3'dATP) and Ca(2+).

9 pre-steady-state kinetic analysis using a 3'-dATP analogue in place of ATP shows that removal of this

10 ibitor (Ki, 10.7 nM), followed by 2'-MANT-3'-dATP [2'-O-(N-methylanthraniloyl)-3'-deoxy-ATP] (Ki, 16.

11 Reasonable binding modes of 2'-MANT-3'-dATP and bis-(M)ANT-nucleotides at sGC alpha1beta1 requi

12 amma-CXN(3) dGTP (5-6) and alpha,beta-CXN(3) dATP (7-8) analogues are described.

13 Labeling with [alpha-32P]dATP followed by a cold dATP chase has demonstrated that

14 eoxyadenosine-5'-O-(1-thiotriphosphate) as a dATP alternative during DNA strand extension.

15 t bases ranges from a low of 25 microM for a dATP:A mispair to a high of 360 microM for a dCTP:T misp

16 des including A:dCTP, A:dGTP, A(syn):dGTP, A:dATP, A(syn):dATP, T:dCTP, and T:dGTP to study the struc

17 syn):dATP<T:dCTP<A:dGTP<A(syn):dGTP<A:dCTP<A:dATP.

18 y which pol X can preferentially accommodate dATP opposite template oxoG further underscores the role

19 uently elongated by DNA polymerase and added dATP.

20 transcription completely dependent on added dATP.

21 ican swine fever virus incorporates adenine (dATP) opposite to oxoG with higher efficiency than the n

22 That hpol eta discriminates against dATP exclusively at the insertion stage is confirmed by

23 ion between active-site residue Tyr(271) and dATP that causes an anti to syn change in the 8-oxoG (sy

24 mutant RecA protein also hydrolyzes ATP and dATP at rates up to 10-fold higher than either single mu

25 Moreover, ATP and dATP bind at an additional allosteric site 40 A away fro

26 We determined that ATP and dATP bind to NrdR in a negatively cooperative fashion, s

27 ity of the enzyme was only fueled by ATP and dATP.

28 In the presence of cytochrome c and dATP, Apaf-1 assembles into an oligomeric apoptosome, wh

29 Upon binding to cytochrome c and dATP, Apaf-1 oligomerizes into a heptameric complex know

30 the molecular mechanism of cytochrome c- and dATP-mediated activation of Apaf-1.

31 had 100-fold higher dATP concentrations and dATP/TFV-DP ratios, likely reflecting the activated stat

32 h on the incorporation of dCTP (correct) and dATP (incorrect) opposite the G (normal) or 8-oxoG(damag

33 ations in association with elevated dAdo and dATP levels and increased apoptosis in the lung.

34 similar insertion efficiencies for dCTP and dATP.

35 NSAH depresses dGTP and dATP levels in the dNTP pool causing S-phase arrest, pro

36 F1143 and the protein bound to both dGTP and dATP suggested allosteric regulation of its enzymatic ac

37 d that the Dpo4 polymerase inserted dGTP and dATP when challenged by the PdG adduct.

38 polymerases efficiently polymerize dGTP and dATP when tC and tCo are in the template strand.

39 e-primer DNA reveal non-productive (dGTP and dATP) alignments of incoming nucleotide and 8-oxoG.

40 els of DNA precursors, particularly dGTP and dATP.

41 Native human DNA polymerase eta, DNA and dATP were co-crystallized at pH 6.0 without Mg(2+).

42 erent rotameric conformations in TFV-DP- and dATP-bound structures that may help explain how K65R RT

43 Levels of dTTP and dATP were significantly reduced in cls8.

44 decreased dGTP and increased dCTP, dTTP, and dATP pools; aberrant DNA replication; defective expressi

45 ansfer other nucleotides (UTP, CTP, GTP, and dATP) to the acceptor RNA in the absence or presence of

46 n to incorporate both dCTP (no mutation) and dATP (G-->T substitution) opposite 8-oxo-G.

47 heir 2'-OH termini using the enzyme OAS1 and dATP.

48 ation of a Hoogsteen pair between 8-oxoG and dATP less favorable.

49 , dATP insertion opposite an abasic site and dATP misinsertions have common features.

50 ined by reduced levels of the R2 subunit and dATP in p53-deficient cancer cells.

51 gen-bonding interactions between the TTD and dATP than between the TTD and dGTP.

52 ty than ATP, PSTK utilizes GTP, CTP, UTP and dATP as phosphate-donors.

53 oG:anti-dCTP) and Hoogsteen (syn-8-oxoG:anti-dATP) base pairing were clearly visible and were maintai

54 present at fertilization and is activated as dATP is depleted via DNA polymerization.

55 -diphosphate shows a similar binding mode as dATP in the nucleotide-binding site.

56 significantly with only 10% of substrate as dATP.

57 ted that cardiac myosin can use 2-deoxy-ATP (dATP) as an energy substrate, that it enhances contracti

58 Replacing ATP with 2 deoxy-ATP (dATP) increased F-actin speed for both groups by a simil

59 naturally occurring nucleotide 2-deoxy-ATP (dATP) is a myosin activator that enhances cross-bridge b

60 l with elevated skeletal muscle 2-deoxy-ATP (dATP) was used to study how myosin activators may affect

61 Here we show that 2'-deoxy-ATP (dATP), but not 3'-deoxy-ATP, increases the activity of G

62 eoxyribose leads to ligands (mant-deoxy-ATP [dATP], mant-deoxy-ADP) with inverse agonist activity.

63 monstrate that purified cryopyrin binds ATP, dATP, and ATP-agarose, but not CTP, GTP, or UTP, and exh

64 tch, whereas the composite s-site binds ATP, dATP, dTTP, or dGTP and determines which substrate to re

65 igD and its complexes with manganese and ATP/dATP substrates, which reveal a minimized polymerase wit

66 nzyme inhibition based on differences in ATP/dATP consumption.

67 although the Q24A mutant still relied on ATP/dATP for duplex unwinding.

68 P) from the analogous 5'-triphosphate (2'-Az-dATP) onto the tyrosine hydroxyl group of a peptide, whi

69 ight into the discrimination by Dpo4 between dATP and dGTP opposite DFT and its inability to extend b

70 on the go and that negative feedback between dATP and ribonucleotide reductase ensures tight control

71 2+) directly bridges the interaction between dATP and HBV-RT and the other serves as a coordinator to

72 ed protein lacking this cone could only bind dATP to its s-site.

73 in an N-terminal ATP cone domain that binds dATP or ATP and functions as an on/off switch, whereas t

74 Chromatin contacts are captured by biotin-dATP incorporation and proximity ligation, followed by g

75 ed 2'-deoxyadenosine 5'-triphosphate (biotin-dATP) by terminal deoxynucleotidyl transferase (TdT).

76 in close proximity to the sugar of the bound dATP, whereas Arg-504 makes a hydrogen bridge with the b

77 uctures of the T7 helicase domain with bound dATP or dTTP identified Arg-363 and Arg-504 as potential

78 ive of non-productive complex formation, but dATP:8-oxoG insertion events that do occur are 2-fold mo

79 ts by enhancing allosteric RNR inhibition by dATP.

80 tly compelling to consider elevated cardiac [dATP] as a therapeutic option to treat systolic dysfunct

81 g-term (at least 5-mo) elevation of cardiac [dATP] results in sustained elevation of basal left ventr

82 strate/specificity effector-pairs bound (CDP/dATP, UDP/dATP, ADP/dGTP, GDP/TTP) that reveal the confo

83 ccurs with only minor elevation of cellular [dATP].

84 ling with [alpha-32P]dATP followed by a cold dATP chase has demonstrated that newly synthesized DNA o

85 ease in the specificity constant for correct dATP:T incorporation.

86 300-fold slower incorporation of the correct dATP:T relative to that of the wild type led to a 130-fo

87 The method employs Cy5-dATP incorporation into a DNA primer that has been prela

88 However, RecA protein is also a dATPase; dATP supports RecA-RFP nucleoprotein filament formation

89 results suggest that activating the dAdo-DCK-dATP pathway directly results in increased apoptosis in

90 te base opposite to the incoming dNTP (dCTP, dATP, dGTP) is oxoG.

91 used as substitutes for natural dTTP, dCTP, dATP, and dGTP in PCR.

92 ry hpol eta-DNA complexes and incoming dCTP, dATP, or dGTP opposite 8-oxoG reveal that an arginine fr

93 dition, the rate of incorporation of 1-deaza-dATP opposite guanine was decreased 5-fold.

94 Eliminating N(6) from 3-deaza-dATP now greatly reduced incorporation, suggesting that

95 repared by thiol addition to 7-vinyl-7-deaza-dATP.

96 inhibited by endogenous levels of deoxyATP (dATP) present at fertilization and is activated as dATP

97 second, and among natural deoxynucleotides, dATP was the preferred substrate due to its stronger int

98 with mixtures of nucleotides, including dGTP/dATP, dGTP/dCTP, dGTP/dTTP, and dGTP/dUTP.

99 DNA polymerase beta (pol beta) discriminates dATP from dCTP when processing 8-oxoguanine (8-oxoG), we

100 e we report crystal structures of RT-RNA/DNA-dATP and RT-RNA/DNA-nevirapine (NVP) ternary complexes a

101 The polymerase region of RT-RNA/DNA-dATP complex resembles DNA/DNA ternary complexes apart f

102 , and GDP, C site) as well as ATP and dNTPs (dATP, dGTP, TTP) allosteric effectors that control enzym

103 LS process indicated that deoxypurines (i.e. dATP and dGTP) are inserted predominantly opposite 1,N (

104 e presence of the negative activity effector dATP and provide structural support for an active alpha(

105 characterized RNRs, the binding of effector dATP alters the active site to select for pyrimidines ov

106 Enzyme hydrolyzed all rNTPs efficiently, dATP and dCTP with moderate efficiency, while it showed

107 diffraction data indicate that with elevated dATP, myosin heads were extended closer to actin in rela

108 action evidence indicates that with elevated dATP, myosin heads were extended closer to actin in rest

109 c studies indicated that the first blunt-end dATP incorporation was 80-fold more efficient than the s

110 we report the effect of chronically enhanced dATP concentration on cardiac function using a transgeni

111 In nearly 10% of events, dATP would be incorporated, and once incorporated dA (op

112 Exploiting dATP as a molecular probe, we assess how small changes i

113 bound nucleotide (ATP and to a lesser extent dATP) might dictate the requirement for accessory factor

114 the presence of ATP and, to a lesser extent, dATP.

115 ed dGTP, whereas Taq demonstrated a bias for dATP.

116 state kinetics shows diminished "bursts" for dATP:8-oxoG and dCTP:8-oxoG incorporation, indicative of

117 tudies, abasic sites preferentially code for dATP insertion (the "A rule").

118 but with a nearly 2-fold longer duration for dATP or dTTP incorporation than for dCTP or dGTP into co

119 The apparent Km for dATP at site 2 is approximately 10 mum for mouse and 1 m

120 an abasic site, with kinetic preference for dATP as the substrate.

121 260-2900-fold and exhibited a preference for dATP incorporation.

122 suggest that the significant preference for dATP insertion observed experimentally can result from t

123 ts affinity for dsDNA and its preference for dATP over ATP.

124 results show that Arg-363 is responsible for dATP, dCTP, and dGTP hydrolysis, whereas Arg-504 and Ser

125 otential determinants of the specificity for dATP and dTTP.

126 ited incorporation, whereas removing N3 from dATP dramatically increased incorporation (32-fold).

127 Similarly, syn-Fapy.G:dATP pairing showed greater stacking in the 5'-TGT seque

128 In pol beta, the syn-8-oxo-G:dATP pair showed greater stacking with the 3'-T:A base p

129 catalytic core tetramer in complex with GTP/dATP, revealing the structural basis of GTP-mediated SAM

130 with 1 mm deoxyadenosine accumulates higher dATP levels than mammalian cells but that this effect di

131 ssue, rectal lymphocytes had 100-fold higher dATP concentrations and dATP/TFV-DP ratios, likely refle

132 lity and obtain greater understanding of how dATP substitution results in contractile enhancement, we

133 rison with autoinhibited Apaf-1 revealed how dATP binding triggers a set of conformational changes th

134 complexes revealed incoming non-hydrolyzable dATP or dGTP analogs not pairing with but instead in a s

135 Our results identify dATP/TFV-DP ratios as a possible correlate of protection

136 alog combining the chemical modifications in dATP and P-ATP.

137 -3', a diffracting ternary complex including dATP was obtained.

138 aged in a nascent base pair with an incoming dATP analog.

139 - N (2)-dG ([BP]G*), reveal that an incoming dATP is significantly preferred over the other three dNT

140 complex of the R283K mutant with an incoming dATP-analogue and templating 8-oxoG resembles a G-A mism

141 rming a Hoogstein base pair with an incoming dATP.

142 preventing Gh from pairing with the incoming dATP or dGTP base.

143 ue interaction between 5ClC and the incoming dATP, which would facilitate mutagenic lesion bypass.

144 analysis, hpol eta preferred to incorporate dATP and dGTP, compared with dTTP.

145 BF incorporates dATP opposite 8-oxoG less efficiently, and modifying N1,

146 NA synthesis by preferentially incorporating dATP opposite the noninstructional lesion.

147 corporation opposite the adduct by inserting dATP or dTTP.

148 mpanied by the accumulation of intracellular dATP.

149 hen only 10% of the contractile substrate is dATP.

150 ploits tailed primers and ferrocene labelled dATP analogue to produce PCR products that can be direct

151 s efficient base stacking between the lesion:dATP pair and the 3'-A:T base pair in the 5'-TGA sequenc

152 f the cell cycle and preferentially lowering dATP levels.

153 n an error-prone fashion by inserting mainly dATP.

154 Inverse agonist activity of mant-dATP was found at the WT human P2Y(12) and half of the c

155 Thus, N-MC-dATP corrects the error-prone nature of hpol iota by pre

156 ternary complex of hpol iota inserting N-MC-dATP opposite dT reveals that the adenine ring is stabil

157 nocarba-2'-deoxyadenosine triphosphate (N-MC-dATP).

158 less catalytically favorable than anti N-MC-dATP.

159 Unmodified dATP and South-MC-dATP each adopt syn glycosyl orientations to form Hoogst

160 with the natural dATP substrate, we measured dATP contents in peripheral lymphocytes, lymphoid tissue

161 N6-methyl-dATP activity is present in MTH1 homologues from distant

162 Of note, N6-methyl-dATP activity is unique to MTH1 among related NUDIX hydr

163 that modified nucleotides such as N6-methyl-dATP and O6-methyl-dGTP are incorporated opposite an aba

164 zebrafish eggs microinjected with N6-methyl-dATP compared with noninjected embryos.

165 tive-site subpocket explaining why N6-methyl-dATP is a good MTH1 substrate.

166 We show that N6-methyl-dATP is incorporated into DNA in vivo, as indicated by i

167 iently catalyzes the hydrolysis of N6-methyl-dATP to N6-methyl-dAMP and further report that N6-methyl

168 incorporation of matched dCTP or mismatched dATP.

169 olymerization product with the thio-modified dATP as a substrate.

170 transcription by competing with the natural dATP substrate, we measured dATP contents in peripheral

171 he applicability of the N(6)-(2-nitrobenzyl)-dATP as an ideal RT for CRT sequencing.

172 The 3'-OH group of the N(6)-(2-nitrobenzyl)-dATP remains unblocked, providing favorable incorporatio

173 omplex) and with an incoming nonhydrolyzable dATP analog (ternary complex) were solved.

174 ry is hampered when the incorrect nucleotide dATP is bound opposite 8-oxoG; the unfavorable interacti

175 depending on the availability of nucleotide dATP/ATP.

176 vely), and competing endogenous nucleotides (dATP and dCTP) in 47 healthy women.

177 In the absence of dATP or ATP, Dronc zymogen potently induces formation of

178 ytidine kinase prevented the accumulation of dATP and restored thymocyte differentiation and prolifer

179 osis as a consequence of the accumulation of dATP derived from thymocytes failing the positive/negati

180 showed that hpol eta favors the addition of dATP and dGTP opposite 1,N (6)-erA.

181 ers and hexamers, was induced by addition of dATP or dGTP, but not of dTTP or dCTP.

182 f hpol eta with a non-hydrolyzable analog of dATP or dGTP opposite an abasic site, H-bonding was obse

183 f the R2 subunit extends the availability of dATP in the G(2)/M phase to promote the repair of NER-me

184 he calculations indicate that the binding of dATP to the enzyme-DNA complex is thermodynamically favo

185 dimer successfully acts as a combination of dATP and dTTP in primer extension reactions, and the dGp

186 osphate to mimic the active-site contacts of dATP may explain its effective inhibition of RT and main

187 s an effector, activating the degradation of dATP and dCTP with dGTP also being consumed in the react

188 es the energy derived from the hydrolysis of dATP in addition to dTTP for mediating DNA unwinding.

189 orporation, suggesting that incorporation of dATP (analogues) opposite 8-oxoguanine proceeds via a Ho

190 -diphosphate, inhibited the incorporation of dATP into a primed DNA template by the EBV DNA polymeras

191 o the reduced efficiency of incorporation of dATP opposite T, and that the autosomal dominant phenoty

192 pol/ K d (dNTP) values for the insertion of dATP and dGTP opposite 7-deazaadenine and 7-deazaguanine

193 bserved for hpol iota-catalyzed insertion of dATP opposite dT.

194 of deoxyadenosine accumulates high levels of dATP in an adenosine kinase-dependent process and dies w

195 ely small (but supraphysiological) levels of dATP increase the number of strongly attached, force-pro

196 MP and further report that N6-methylation of dATP drastically increases the MTH1 activity.

197 oxanthine base pairs by pol alpha, and N3 of dATP again helps prevent polymerization opposite a templ

198 imately 50% conversion) by polymerization of dATP using yeast PaP, and the short DNA strand appended

199 inhibited R1-R2 octamers in the presence of dATP but, unlike the E. coli enzyme, cannot be turned of

200 In the presence of dATP, glycerol, and Tris buffer, the DNA primase isolate

201 olysis of dTTP without affecting the rate of dATP hydrolysis.

202 To determine the minimal ratio of dATP/ATP (constant total NTP) that significantly enhance

203 in of Y115 more than does the ribose ring of dATP, possibly picking up a favorable pi-pi interaction.

204 We used a series of dATP and dGTP analogues to determine how DNA polymerase

205 r NTPs, resulting in the preferential use of dATP and the use of dCTP, a nucleotide not normally used

206 ues elongation after inserting oxidized 2-OH-dATP or therapeutic 6-thio-dGTP, but insertion disrupts

207 Delivery of 8-oxo-dGTP and 2-OH-dATP to zebrafish embryos was highly toxic in the absenc

208 At pH 6.5, only dATP was inserted, but as the pH increased to 9.0, the a

209 ite 2 and activate SAMHD1, but in cells only dATP or dTTP are present at sufficient concentrations.

210 nzymes, that monitors the decrease of ATP or dATP in real time, allowing detection of enzyme inhibiti

211 in vitro with the addition of CC and ATP or dATP to cell lysates.

212 he enzyme on or off by the binding of ATP or dATP, respectively.

213 ed that Mtr4p can, in the presence of ATP or dATP, unwind the duplex region of a partial duplex RNA s

214 nt protein can bind but not hydrolyze ATP or dATP.

215 ty was dependent upon the presence of ATP or dATP.

216 g DNA helicase activity that required ATP or dATP.

217 rotein filaments but do not hydrolyze ATP or dATP.

218 presence of adenosine triphosphate (ATP) or dATP whereas the non-hydrolyzable gamma-S-ATP does not s

219 not modulate a preference for either dCTP or dATP when opposite 8-oxodG in single-nucleotide gapped D

220 g sites, site 1 binding dGTP, site 2 dGTP or dATP.

221 n orientation whether GS-9148-diphosphate or dATP is bound.

222 The binding of either GS-9148-diphosphate or dATP to the binary RT-DNA complex resulted in the finger

223 lexes with tenofovir diphosphate (TFV-DP) or dATP.

224 Dpo4 capable of incorporating dCTP, dTTP or dATP opposite the adduct reasonably well.

225 ATP but not ADP, AMP, GTP, or dATP specifically protected Hbr1p from proteolysis by tr

226 e beta preferentially incorporated dCTP over dATP, DNA polymerase lambda did not modulate a preferenc

227 ATP, while polymerase alpha favors dGTP over dATP by a factor of 30-65.

228 cture of the L561A variant forming an 8-oxoG.dATP mispair and show that the propensity for forming th

229 The new analog P-dATP can be not only an invaluable tool to study CFTR ga

230 hesized N(6)-(2-phenylethyl)-2'-deoxy-ATP (P-dATP), an analog combining the chemical modifications in

231 analogue, N6-(2-phenylethyl)-2'-deoxy-ATP (P-dATP), can increase the open probability (Po) to approxi

232 Interestingly, P-dATP completely rectified the gating abnormality of Delt

233 tion effect, suggesting that the action of P-dATP takes place at the interface of both NBDs.

234 We show that P-dATP binds to ABP1 to potentiate the activity of G551D,

235 ctivation of RNR upon commitment to S phase, dATP feedback inhibition ensures that the dNTP concentra

236 strongly discriminated against polymerizing dATP opposite 8-oxoG, and removing N1, N(6), or N7 furth

237 experimentally can result from two possible dATP incorporation modes.

238 it is noteworthy that Dpo4 strongly prefers dATP opposite DFT over dGTP (approximately 200-fold) and

239 TP activates the enzyme solely by preventing dATP from binding.

240 In principle, dATP feedback inhibition should be sufficient to couple

241 In contrast, RecA . dATP efficiently catalyzes strand exchange even in the a

242 cleotide excision repair (NER) and a reduced dATP level in the G(2)/M phase of the cell cycle.

243 48R and E96D single mutant proteins restores dATP hydrolysis to 25% of the wild type rate, with maxim

244 l the atomistic structures of HBV-RT/DNA-RNA/dATP and HBV-RT/DNA-RNA/TFV-DP (tenofovir diphosphate) c

245 dynamics (MD) simulations of HBV-RT/DNA-RNA/dATP complexes revealed strong coupling of the natural n

246 ukaryotic and Escherichia coli class I RNRs, dATP inhibits enzyme activity through the formation of i

247 dependent complex with RNR, which stabilizes dATP in the activity site of RNR and thus inhibits the e

248 more efficiently than the natural substrate, dATP.

249 oupling of the natural nucleotide substrate, dATP, to the active site of the RT, and the differential

250 e system insertions by pol mu: T:dGTP<A(syn):dATP<T:dCTP<A:dGTP<A(syn):dGTP<A:dCTP<A:dATP.

251 A:dCTP, A:dGTP, A(syn):dGTP, A:dATP, A(syn):dATP, T:dCTP, and T:dGTP to study the structure-function

252 es an anti to syn change in the 8-oxoG (syn):dATP complex explains this slow motion, in contrast to t

253 eading to predominantly single non-templated dATP incorporation.

254 dGTP with a 4-9-fold higher probability than dATP, while polymerase alpha favors dGTP over dATP by a

255 The calculations also illustrate that dATP binding is thermodynamically favored over dGTP bind

256 Furthermore, the simulations indicate that dATP and dTTP are better incorporated in the damaged sys

257 bserved, steady-state kinetic data show that dATP insertion opposite DFT is greatly inhibited relativ

258 We and others have previously shown that dATP increases contractile force in normal hearts and mo

259 ions of post-powerstroke myosin suggest that dATP induces structural changes in myosin heads that inc

260 ions of post-powerstroke myosin suggest that dATP induces structural changes in myosin that increase

261 The dATP can be inserted opposite the T on the 5' side of th

262 The dATP did not pair with PdG, but instead with the 5'-neig

263 The dATP-induced inactive form is an alpha4 complex, which c

264 The dATP-mediated structural alterations in myosin reported

265 The dATP/dGTP insertion ratio opposite the dGh/dIa site as a

266 r ATPase activity than that of wt ABCG2, the dATP-dependent methotrexate transport activities of thes

267 In addition, the dATP can be misincorporated opposite the adduct.

268 These structures reveal that binding of the dATP analog induces a closed polymerase conformation, an

269 This study of the dATP-induced changes in myosin may be instructive for de

270 Examination of the active site with the dATP substrate suggests an in-line nucleophilic attack o

271 in sliding speed linearly increased with the dATP/ATP ratio (p < 0.01 at 10% dATP).

272 Each alpha polypeptide binds three dATP molecules, and the N-terminal ATP cone is critical

273 Thus, dATP insertion opposite an abasic site and dATP misinser

274 tive concentration (EC90) ratios of TFVdp to dATP and FTCtp to dCTP (alone and in combination) for pr

275 ucleotide 2-deoxy-adenosine 5'-triphosphate (dATP) can be used by cardiac muscle as an alternative en

276 th incorrect deoxyadenosine 5'-triphosphate (dATP).

277 to incorporate deoxyadenosine triphosphate (dATP) at the 3'-OH of an RNA molecule, followed by termi

278 phosphate or 2'-deoxyadenosine triphosphate (dATP), and a post-incorporation structure with GS-9148 t

279 -position of 2'-deoxyadenosine triphosphate (dATP), which, upon incorporation, terminates DNA synthes

280 n IRBIT forms a deoxyadenosine triphosphate (dATP)-dependent complex with RNR, which stabilizes dATP

281 osphorylated to deoxyadenosine triphosphate (dATP).

282 a free deoxyribose nucleotide triphosphate, dATP or dGTP, to Pol eta complexed with undamaged or dam

283 cificity effector-pairs bound (CDP/dATP, UDP/dATP, ADP/dGTP, GDP/TTP) that reveal the conformational

284 Unmodified dATP and South-MC-dATP each adopt syn glycosyl orientati

285 esults in contractile enhancement, we varied dATP/ATP ratio in porcine cardiac muscle preparations.

286 When coupled with various dATP analogs (e.g., radioactive, fluorescent, affinity t

287 de conformations and contacts for ATP versus dATP are observed in the cocrystals, the functional anal

288 t form in the presence of ATP but do so when dATP is provided.

289 With dATP, myosin heads may remain in an activated state near

290 Hoogsteen edge, which can hydrogen-bond with dATP or dGTP.

291 crystals permit substitution of TFV-DP with dATP at the dNTP-binding site.

292 P is proficient for DNA strand exchange with dATP or at lower pH.

293 is on a dT template) that were extended with dATP by Escherichia coli DNA polymerase I.

294 tension redevelopment (k(TR)) increased with dATP at all Ca(2+) levels.

295 k(TR) increased linearly with dATP/ATP ratio at pCa 4.5 and 5.5.

296 .5), isometric force increased linearly with dATP/ATP ratio, but at submaximal Ca(2+) (pCa 5.5) this

297 nsertion complexes for the Y567A mutant with dATP and dCTP opposite a templating 8-oxoG in a 13/18mer

298 einsertion complex for the Y567A mutant with dATP opposite a templating Gh (R-configuration) in a 13/

299 GTP also being consumed in the reaction with dATP.

300 ions before and after the chemical step with dATP and dCTP opposite an 8-oxoG template started from p