ライフサイエンスコーパス: dT

1 dT formed no cross-links, nor did Arg, Gln, Tyr, or Asn.

2 assessed two therapies in Tk2(-/-) mice: (1) dT+dC and (2) coadministration of the deaminase inhibito

3 ded DNA conjugates with the sequence (dA)10.(dT)10 and hexaethylene glycol linkers at one end (hairpi

4 ntervening residues in the loop), dT, dT(2), dT(3), dTTA, and dT(4)] were considered through MD simul

5 e ( approximately 7-7.5) with poly(dA).2poly(dT) as compared to neomycin ( approximately 6.5).

6 perature (T(m3-->2)) at which poly(dA).2poly(dT) dissociated into poly(dA).poly(dT) and poly(dT) incr

7 y(dT) DNA duplex than for the poly(dA).2poly(dT) DNA triplex.

8 ty of all tested ligands with poly(dA).2poly(dT) increased in the following order: neomycin < 1 < 3 <

9 lator-neomycin conjugates for poly(dA).2poly(dT) increases as a function of the surface area of the i

10 the increment of T(m3-->2) of poly(dA).2poly(dT) induced by neomycin was negligible under the same co

11 r conjugates (1-4) stabilized poly(dA).2poly(dT) much more than its parent compound, neomycin.

12 y to neomycin, 3 destabilizes poly(dA).2poly(dT) triplex but stabilizes poly(dA).poly(dT) duplex, sug

13 -neomycin conjugates (1-4) to poly(dA).2poly(dT) was also confirmed by competition dialysis and a flu

14 binding of compounds 1-4 with poly(dA).2poly(dT) was mostly enthalpy-driven and gave negative DeltaC(

15 0.3) x 10(8) M(-1)] of 2 with poly(dA).2poly(dT) was the highest, almost 1000-fold greater than that

16 ator-neomycin conjugates with poly(dA).2poly(dT) were derived from an integrated van't Hoff equation

17 a DNA polynucleotide triplex [poly(dA).2poly(dT)] were conducted.

18 tL dimer binds to an 18 bp duplex with a 3'-(dT(20)) single-stranded flanking region, with apparent a

19 ing noncomplementary twin 5'-(dT)(6) and 3'-(dT)(6) tails, and unwinding can be described by a simple

20 il is extended to 10 nt [5'-(dT)(10) and 3'-(dT)(6)], the DNA end structure for which RecBCD displays

21 DNA, RecBCD unwinding of DNA possessing 3'-(dT)(6) and 5'-(dT)(6) noncomplementary ssDNA tails is we

22 ementary single-stranded (ss) DNA tails [3'-(dT)(6) and 5'-(dT)(6) or 5'-(dT)(10)] on the mechanism o

23 ults showed that BrdU replaced more than 30% dT in genomic DNA after the cells were treated with 10 m

24 reases polymerase activity of gp5 on dA(350)/dT(25).

25 the HB pattern and base pair geometry of 3DA/dT is exactly the same as those of dA/dT, which makes 3D

26 d selectively to the PPT near the 5'(rA)(4):(dT)(4) tract and the 3' PPT-U3 junction.

27 winding of DNA possessing 3'-(dT)(6) and 5'-(dT)(6) noncomplementary ssDNA tails is well described by

28 -stranded (ss) DNA tails [3'-(dT)(6) and 5'-(dT)(6) or 5'-(dT)(10)] on the mechanism of RecBCD and Re

29 tary 5' ssDNA tail is extended to 10 nt [5'-(dT)(10) and 3'-(dT)(6)], the DNA end structure for which

30 DNA tails [3'-(dT)(6) and 5'-(dT)(6) or 5'-(dT)(10)] on the mechanism of RecBCD and RecBC unwinding

31 y of RecBC for DNA ends possessing 3' or 5'-(dT)(n) ssDNA tails with n<6 nt, with the relative enhanc

32 DNA end possessing noncomplementary twin 5'-(dT)(6) and 3'-(dT)(6) tails, and unwinding can be descri

33 After the addition of a dT residue to the DNA primer, which is specified by the

34 le in bypassing alpha-dC, alpha-dG and alpha-dT in vivo.

35 ha-dC and triggered T-->A mutation for alpha-dT.

36 One of these dNTP analogs (dT) was demonstrated to fit into DNA polymerase beta (DN

37 es has revealed that the insertion of dA and dT was catalyzed by different polymerases in cells.

38 d tendency of hpol eta to insert both dC and dT opposite the O(6)-MeG lesion with similar efficiencie

39 X-ray crystal structures of both dC and dT paired with O(6)-MeG were solved in both insertion an

40 ed as a contaminant in the commercial dG and dT 3'-monophosphate samples.

41 During preparation of the dG and dT derivatives of alpha,beta-methylene diphosphate, we a

42 es in the loop), dT, dT(2), dT(3), dTTA, and dT(4)] were considered through MD simulation and free en

43 l conduction to this model altered %dLV and %dT to 13.1% and 10.1%, respectively.

44 lar-paced canine model resulted in %dLV and %dT values of -7.1% and 1.5%, in contrast to the experime

45 cardiac anatomy is responsible for %dLV and %dT values of 7.4% and 5.5%, respectively.

46 d as %dLV=100x(LVLATep-LVLATen)/LVLATep and %dT=100x(TLATep-TLATen)/TLATep, respectively).

47 nd between binding modes using (dT)(70) and (dT)(140).

48 showed a substantial proportion of apoptotic dT cells.

49 n of gal1 as well as periglandular apoptotic dT foci that colocalized with dNKs.

50 pha, beta)-imido]triphosphate (approximating dT) at both the insertion and extension stages with form

51 ave a more stable N-glycosidic bond (such as dT).

52 antly different from that of the cognate ATP/dT scaffold ( approximately 130-fold decrease in kpol).

53 e for UTP/dT incorporation compared with ATP/dT incorporation.

54 ino(bc-araT)-version of bicyclothymidine (bc-dT) has been achieved.

55 ine) and/or have a stable N-glycosylic bond (dT).

56 bstrates that are larger (bromodeoxyuridine, dT) or have a more stable N-glycosidic bond (such as dT)

57 When the opposing dA is replaced by dT, the activity of the adenine can be rescued by adding

58 The pressure dependence of T(c) (dT(c)/dP) becomes 0.91 K/GPa and 0.75 K/GPa from magneti

59 ed to values expected from the slopes dB(c2)/dT approximately 2 T K(-1) near T(c), particularly at lo

60 an incorporate A, G, C, or T opposite the C5-dT-conjugated DNA-peptide conjugates, whereas human poly

61 rstanding how decidual CD8(+) T cell (CD8(+) dT) cytotoxicity is regulated and how these cells integr

62 However, CD8(+) dT degranulated, proliferated, and produced IFN-gamma, T

63 xpression analysis of effector-memory CD8(+) dT demonstrated a mixed transcriptional signature of T c

64 ance between transient dysfunction of CD8(+) dT that are permissive of placental and fetal developmen

65 the decidual microenvironment reduces CD8(+) dT effector responses to maintain tolerance to fetal ant

66 vitro stimulation, demonstrating that CD8(+) dT are not permanently suppressed and retain the capacit

67 mmortalized human endometrial stromal cells (dT-HESCs) following infection with GBS strains from sept

68 form two hydrogen bonds with a complementary dT residue, is reported.

69 rature for the EXT monolayer, with dPi(crit)/dT approximately 1.5 mN/m/ degrees C, but the mixing pre

70 amounts of 3,5'-cyclo-dG (16) and 2,5'-cyclo-dT (17), respectively.

71 2x binding site (O1) on attL as well as a dA+dT-rich upstream element that is required for Orf2x inte

72 orporation of dCTP with templating bases dA, dT, and dC over correct dNTPs.

73 phabet, comprised of just two base pairs (dA-dT and dG-dC), is conserved throughout all life, and its

74 The poly (dA-dT) tracts affect but do not deplete nucleosomes in S. p

75 le-stranded DNA, such as poly(dA-dT)*poly(dA-dT) [poly(dA-dT)], can also induce IFN-beta, but the und

76 Here, we show that the cytosolic poly(dA-dT) DNA is converted into 5'-ppp RNA to induce IFN-beta

77 for synthesizing 5'-ppp RNA from the poly(dA-dT) template.

78 finity where the binding of HMGA2 to poly(dA-dT)(2) is enthalpy-driven and to poly(dA)poly(dT) is ent

79 B form double-stranded DNA, such as poly(dA-dT)*poly(dA-dT) [poly(dA-dT)], can also induce IFN-beta,

80 Biochemical analyses showed that poly(dA-dT)-activated AIM2 inflammasomes induce autophagy and IL

81 NA, such as poly(dA-dT)*poly(dA-dT) [poly(dA-dT)], can also induce IFN-beta, but the underlying mecha

82 i4) reveal that external binding to [poly(dA-dT)]2 becomes important when a fourth meso substituent i

83 uted porphyrin H2Tri4 combines with [poly(dA-dT)]2, [poly(dG-dC)]2, or salmon testes DNA.

84 ompetitive for a flexible host like [poly(dA-dT)]2.

85 natural base pair, and when combined with dA-dT and dG-dC, it provides a fully functional six-letter

86 of 3DA/dT is exactly the same as those of dA/dT, which makes 3DA an optimal analogue for probing mino

87 The opening and the stability of each rU-dA/dT-dA base pair in the two structures are characterized

88 These results suggest that the central rU-dA/dT-dA base pairs in the adenine tract make the largest e

89 high transcription and long homopolymeric dA:dT tracts.

90 of a hydrogen bond for a halogen bond in dA:dT and dG:dC base pairs, which allows 1 or 2 hydrogen bo

91 production of IFN-beta triggered by poly (dA:dT) or HSV-1 requires IFNAR signaling.

92 t is particularly strong at repeated poly(dA:dT) and poly(dC:dG) tracts.

93 RSC clears promoters by translating poly(dA:dT) into directional nucleosome removal.

94 omplexes loaded onto the locus via a poly(dA:dT) tract in the gene promoter and mediated cohesion bef

95 f the PHO5 promoter that introduce a poly(dA:dT) tract-stimulated gene expression under nonpermissive

96 First, poly(dA:dT) tracts are localized in a strand-dependent manner, w

97 Second, poly(dA:dT) tracts are preferentially "capped" by G:C residues o

98 ng deserves to be singled-out: short poly(dA:dT) tracts are reported in the literature as fundamental

99 cross-linking, we show that internal poly(dA:dT) tracts do not block the engagement of the ATPase mot

100 el in which localized and G:C-capped poly(dA:dT) tracts initiate or facilitate the formation of NFRs

101 nd equilibrium experiments show that poly(dA:dT) tracts perturb remodeling reactions if within one an

102 racterized by positioned patterns of poly(dA:dT) tracts with several novel features.

103 /MARs are preferentially enriched in poly(dA:dT) tracts, sequences that resist nucleosome formation,

104 a closely knit relationship between poly(dA:dT) tracts, their capping patterns, and the central coor

105 tion at promoters is maintained over poly(dA:dT) tracts, whereas internucleosome spacing and all othe

106 , including lipid A, LPS, poly(I:C), poly(dA:dT), and cGAMP, induce cGAS expression in an IFN-I-depen

107 analyzing the dynamic expression of poly(dA:dT)-induced IFN-beta and cGAS transcripts, we have found

108 xpressed DNA sensor DDX41 attenuates poly(dA:dT)-triggered IFN-beta production and cGAS induction.

109 cients (using McMillan-Mayer theory), and dB/dT parameters have also been determined and discussed in

110 The dB/dT values suggest that L-glycine and beta-alanine act as

111 We observed that dC+dT delayed disease onset, prolonged life span of Tk2-def

112 al structures of N7mdG or dG paired with dC, dT, dG, and dA.

113 usly recognizes two decadeoxyoligothymidine (dT(10)) tracts to form triplexes with a peptide-DNA stra

114 DNA containing a template 2'-deoxythymidine (dT) paired with an incoming dNTP or modified nucleotide

115 nucleoside monophosphates to deoxythymidine (dT) and deoxycytidine (dC), we hypothesized that: (1) de

116 , deoxyuridylate (dU), and deoxythymidylate (dT), and have found that the macroscopic rate of translo

117 parison between 40-mers of deoxythymidylate (dT(40)) and uridylate (rU(40)) measured using the powerf

118 tion dispersion, we show here that wobble dG*dT and rG*rU mispairs in DNA and RNA duplexes exist in d

119 on dispersion recently showed that wobble dG.dT and rG.rU mismatches in DNA and RNA duplexes transien

120 in the order M(1)dG:dC > M(1)dG:dG > M(1)dG:dT approximately M(1)dG:dA, but neither hPol iota nor Re

121 not extend well past an N (7)-CH(3) 2'-F dG:dT mispair.

122 precisely the mutagenic arrangement of dGTP:dT normally preferred by hpol iota.

123 the rates of interannual tropical-mean dOLR/dTs and global-mean dP/dTs, consistent with the muted tr

124 sabling PVC for 4 weeks, LVEF (p = 0.01), dP/dT (p = 0.047), and resting VNA (p = 0.03) recovered to

125 er 12 weeks of PVCs, LVEF (p = 0.006) and dP/dT (p = 0.007) decreased.

126 e eclogite facies boundary has a positive dP/dT, cooling from supra-solidus conditions (T > 950 mascu

127 lue of the polarization/temperature ratio dP/dT at the phase transformation is increased by 10%.

128 ee with the observation-based interannual dP/dTs all predict dP/dTs under global warming higher than

129 bal warming higher than the ensemble mean dP/dTs from the approximately 20 models analysed in this st

130 al tropical-mean dOLR/dTs and global-mean dP/dTs, consistent with the muted tropical high cloud shrin

131 tion-based interannual dP/dTs all predict dP/dTs under global warming higher than the ensemble mean d

132 n precipitation per unit surface warming (dP/dTs) for both interannual variability and global warming

133 ., no intervening residues in the loop), dT, dT(2), dT(3), dTTA, and dT(4)] were considered through M

134 the MM > PM probes had either a dThymidine (dT) or a dCytidine (dC) at the 13th position of the prob

135 trate a 50% reduction in action potential dV/dT, a 50-75% reduction in SCN5A, KCNJ2, and CACNA1C ion

136 ells could reflect a considerable endogenous dT source, making the resistance phase a delay of acute

137 t the 4' carbon (4'C-methyl dT and 4'C-ethyl dT).

138 ucleotide with 5-OH-dC, 5-propyne-dC, furano-dT, 1-(2'-deoxy-beta- d-ribofuranosyl)-2-oxo-7-deaza-8-m

139 tes were dC(6) approximately dA(6) > dG(6) > dT(6), correlating with the gas-phase acidities of nucle

140 atal macaque monkeys exposed to either [(3)H]dT or BrdU as embryos.

141 ers of DNA synthesis, [(3)H]thymidine ([(3)H]dT) and the later developed analog bromodeoxyuridine (Br

142 eplication marker tritiated thymidine ([(3)H]dT, or TdR) at early embryonic ages were killed at diffe

143 ore natural DNA constituent nucleotide [(3)H]dT.

144 3)H]-, [2'S-(3)H]-, [4'-(3)H]-, and [5'-(3)H]dTs provided values of 1.033 +/- 0.002, 1.004 +/- 0.002,

145 led that p38 promotes programmed necrosis in dT-HESCs.

146 d eccentric remodeled anatomies resulted in %dT values of 15.6% and 1.3%, respectively.

147 d the glycosidic bond to syn and incorporate dT via a Hoogsteen pair.

148 wever, pol gamma preferentially incorporated dT opposite the gamma-HOPdA adduct and efficiently exten

149 insensitive to temperature (|depsilon(int) /dT| ~ 10(-4) degrees C(-1) ).

150 anner near room temperature (|depsilon(int) /dT| ~ 8 x 10(-3) degrees C(-1) ), enabling unprecedented

151 ment of Arg104 by a smaller residue allows L-dT to bind deeper into the active site, making space for

152 arget nucleotides that are relatively large (dT and bromodeoxyuridine) and/or have a stable N-glycosy

153 ing a negative slope of their melting lines (dT/dP|coexist<0).

154 e identify such a low-molecular-weight (LMW)-dT source as mostly dTDP-glucose and its derivatives, us

155 cked by the rfbA rffH mutations, lacks a LMW-dT pool, the initial DNA synthesis during T-starvation a

156 to the rffC defect, maintains a regular LMW-dT pool, but cannot recover dTTP from it, and thus suffe

157 ligate poly-dT single-stranded DNA and long dT overhangs in a Ku- and XLF-independent manner, but no

158 (i.e., no intervening residues in the loop), dT, dT(2), dT(3), dTTA, and dT(4)] were considered throu

159 urations with either O(6)-MeG:dC or O(6)-MeG:dT bound compared with the corresponding situations in s

160 bose sugar ring at the 4' carbon (4'C-methyl dT and 4'C-ethyl dT).

161 uctures that form readily in unlinked (dA)n.(dT)n sequences, allowing the excited-state dynamics of s

162 On the other hand, the structure of N7mdG:dT shows that the mispair forms three hydrogen bonds and

163 an intercalated conformer, whereas the NarI-dT/-2 deletion duplex exists as multiple conformers.

164 lving the modified S-cdG.dC and 3'- neighbor dT.dA base pairs.

165 ne abolished TLS associated with dA, but not dT, insertion.

166 Arsenolysis of dT by hTP permits kinetic isotope effect (KIE) analysis

167 TDG cleaves the N-glycosylic bond of dT and some other nucleotides, including 5-substituted 2

168 alyzes a slow hydrolytic depyrimidination of dT yielding thymine and 2-deoxyribose (dRib).

169 almost exclusively directed incorporation of dT and dA.

170 This facilitates correct incorporation of dT via a Watson-Crick pair.

171 While insertion of dT can be catalyzed by polymerase eta, kappa, and iota,

172 ults also suggest that the high stability of dT-dA base pairs in the DNA provides a signal for the pa

173 Purification protocols using oligo dT's, locked nucleic acid substituted dT's, and tetramet

174 ated spermatogonia were captured with oligo (dT)-conjugated beads after UV-crosslinking and profiled

175 pic translocation rates of 3 nt s(-1) (oligo(dT)), 35 nt s(-1) (oligo(dU)), and 42 nt s(-1) (oligo(rU

176 titis C virus polymerase using poly(A)/oligo(dT(12)) that were or were not preannealed.

177 atures (T(m) values), however, poly(A)/oligo(dT(12-18)) is not expected to form stable duplexes.

178 cted in reactions containing either an oligo(dT) primer alone, or in reactions supplemented with a DE

179 nizes and pauses at its terminator, an oligo(dT) tract in non-template DNA, terminates 3' oligo(rU) s

180 preannealing reactions for poly(A) and oligo(dT(12)), making it possible to characterize mechanism of

181 f locked nucleic acid (LNA)-containing oligo(dT) probes to poly(A) tails to maximize RNA capture sele

182 re (RIC) uses in vivo UV crosslinking, oligo(dT) capture, and proteomics to identify RNA-binding prot

183 it enzyme retains activity on poly(dA)/oligo(dT) templates but is impaired in its ability to extend s

184 ) lengths or in the elution profile of oligo(dT)-bound targets.

185 We show that sequencing of oligo(dT)-selected polyadenylated mRNAs, without considering t

186 Analyses of the protein composition of oligo(dT)-selected UV photocross-linked ER protein-RNA adducts

187 ency of the thymidine oligonucleotide, oligo(dT)25, and providing a reliable surface for the amplific

188 either template RNA poly(A) or primer oligo(dT(12)) independently.

189 n vivo UV-crosslinking of RNA to RBPs, oligo(dT) capture and mass spectrometry yielded 1,145 differen

190 RNase H can even effectively replace oligo(dT)-based methods for standard RNA-seq.

191 supernatant are separated by a second oligo(dT) selection.

192 h precision and efficiency on a simple oligo(dT) tract, independent of other cis-elements or trans-fa

193 These results demonstrate that oligo(dT) primed 5' capture scRNAseq can detect DENV RNA and q

194 ing ITC, we find that DrSSB binding to oligo(dT)s with lengths close to the determined site size (50-

195 he isolation of polyadenylated RNA via oligo(dT), it will not provide RNA-binding information on prot

196 rotein-RNA complexes are purified with oligo(dT) magnetic beads.

197 An oligo-dT adapter incorporating a dUTP-containing PCR primer pr

198 First, an oligo-dT linked to an adaptor sequence is used to prime cDNA s

199 entify 3' ends, most techniques use an oligo-dT primer to construct deep sequencing libraries.

200 Polyadenylated mRNAs were captured by oligo-dT primers and processed into adapter-ligated cDNA libra

201 features flanking 3' ends derived from oligo-dT-based sequencing, we developed a naive Bayes classifi

202 better identify mis-priming events in oligo-dT primed sequences.

203 mRNA from genomic DNA using a modified oligo-dT bead capture and the respective whole-transcriptome a

204 The first using oligo-dT primers after polyadenylation of the bacterial RNA, t

205 ification, for the cDNA generated with oligo-dT and/or random oligonucleotide primers.

206 Ps were attached with 5'-NH(2)-tagged oligo-(dT)(25) primer and were used to isolate mRNA from breast

207 e dimers in the all-thymine oligonucleotide (dT)(18) are fully formed in <1 ps after ultraviolet exci

208 Using a set of oligonucleotides (dT) with varying lengths as a molecular ruler and also a

209 binding, (SSB)(35) and (SSB)(65) formed on (dT)(70), with rates of interconversion on time scales th

210 he incorporation efficiency of dAMP opposite dT decreased 10(2)-10(3)-fold even when only one minor g

211 arameters for the insertion of dAMP opposite dT using primer/templates (P/T)-containing 3DA.

212 ex of hpol iota inserting N-MC-dATP opposite dT reveals that the adenine ring is stabilized in the an

213 ol iota-catalyzed insertion of dATP opposite dT.

214 propensity for GTP misincorporation opposite dT, predicting frequent A-->G errors in RNA with rates o

215 ion stage, with either an incorporated dA or dT opposite 1,N(6)-dA and 2'-deoxythymidine-5'-[(alpha,b

216 laminofluorene (FAAF), and N is either dA or dT.

217 nto template-primers containing either dC or dT residues 5' to the adduct, and the template-primers w

218 CG3*CNATC-3')(5'-GATNCGGCCGAG-3'), N = dC or dT] and -2 deletion [(5'-CTCG1G2CG3*CNATC-3')(5'-GATNGCC

219 G2CG3*CNATC-3')(5'-GATNGCCGAG-3'), N = dC or dT] duplexes, in which G* was either AF [N-(2'-deoxyguan

220 favorably with dC and dA but not with dG or dT.

221 complexes as well as heterodimeric peptide.[dT(10)C(10)T(10)] hairpin structures with triplex stems.

222 and drives the formation of ternary peptide.[dT(10)](2) complexes as well as heterodimeric peptide.[d

223 ntaining juxtaposed dC and 5'-phosphorylated dT deoxynucleotides (substrate 1) yielded kcat and kcat/

224 g this combined approach to poly dA and poly dT, we find that the global properties of these sequence

225 xperiments using streptavidin blocks or poly dT sequences located at either end of the ssDNA substrat

226 nwind double-stranded DNA that had a 3'-poly(dT) overhang as compared with double-stranded DNA with a

227 ared with double-stranded DNA with a 5'-poly(dT) or lacking a poly(dT) tail.

228 d single-stranded DNA (ssDNA) but not a poly(dT) oligonucleotide and using purified recombinant Exo1

229 ded DNA with a 5'-poly(dT) or lacking a poly(dT) tail.

230 oligoriboadenylate is synthesized on a poly(dT) template by a recombinant form of the PRI1 protein a

231 g miRNA-DNA heteroduplexes, antipoly(A)-poly(dT) and anti-S9.6, were used.

232 dissociated into poly(dA).poly(dT) and poly(dT) increased dramatically (>12 degrees C) in the presen

233 imal to transcriptional start sites and poly(dT) tracts lying distal, and collectively define a symme

234 of ssDNA, which binds one tetramer, and poly(dT), which could bind several.

235 T)(2) is enthalpy-driven and to poly(dA)poly(dT) is entropy-driven.

236 ively bind to the triplex DNA poly(dA)-[poly(dT)](2).

237 dA).2poly(dT) dissociated into poly(dA).poly(dT) and poly(dT) increased dramatically (>12 degrees C)

238 has a higher affinity for the poly(dA).poly(dT) DNA duplex than for the poly(dA).2poly(dT) DNA tripl

239 oly(dT) triplex but stabilizes poly(dA).poly(dT) duplex, suggesting the major groove as the binding s

240 -stranded DNA hairpin with 4-20-nt-long poly(dT) loops, with DeltaG(nWC) approximately -2.4 kcal/mol

241 22 genes were upregulated for LTA, LPS, Poly(dT), and Poly(I:C), and 12, 142, 249, and 16 genes were

242 genes were downregulated for LTA, LPS, Poly(dT), and Poly(I:C), respectively, with at least a 1-fold

243 s follows: 3.3+/-0.4 microM nucleotide (poly(dT)), 27+/-2 microM nucleotide (poly(dU)), and 36+/-2 mi

244 -Ag OBD crystallized in the presence of poly(dT)(12) is also reported.

245 lymer phosphorothioate oligonucleotide [Poly(dT)], and polyinosinic-polycytidylic acid [Poly(I:C)].

246 that the occluded site size of DrSSB on poly(dT) is approximately 45 nucleotides under low-salt condi

247 y(A)(+) RNAs from cellular lysates onto poly(dT)-coated sequencing surfaces, followed by on-surface r

248 evisiae RNA using a surface coated with poly(dT) oligonucleotides to capture the RNAs at their natura

249 V has the distinctive ability to ligate poly-dT single-stranded DNA and long dT overhangs in a Ku- an

250 CEL-Seq2, in contrast, makes use of poly-dT primers to reverse transcribe mRNA, followed by linea

251 the reverse transcription (RT) reaction-poly-dT priming, random priming and pooled exon-specific prim

252 gle internal mismatch is rC.dG > rG.dC >> rA.dT > rU.dA.

253 ngle internal mismatch is rG.dC > rC.dG > rA.dT > rU.dA.

254 n specific dynamic properties of the poly(rA/dT) segment and help advance our understanding of the me

255 latter results from rigidity of the poly(rA/dT) tract and leads to base-pair slippage of this sequen

256 reference and incompatibility of the poly(rA/dT) tract of the PPT with the nucleic acid conformation

257 boxamide]-2'-deoxyuridine (Nap-dU) replacing dT.

258 contributions of rA.dA, rC.dC, rG.dG and rU.dT single internal mismatches were measured for 54 RNA/D

259 the single internal mismatches is rG.dG > rU.dT > rA.dA > rC.dC.

260 ative cooperativity for binding of a second (dT)(35) molecule that is evident in Ec-SSB.

261 A similar dT-induced conformational heterogeneity was observed for

262 re-formed 3' and/or 5' single-stranded (ss)-(dT)(n) flanking regions (tails) (n ranging from zero to

263 results are obtained for the single strand (dT)(20) by steady-state and time-resolved optical spectr

264 oligo dT's, locked nucleic acid substituted dT's, and tetramethylammonium chloride salts were charac

265 Decidual T (dT) cells but not peripheral T (pT) cells bound gal1 and

266 is nonlinear with a crossover from dsigma(T)/dT > 0 to dsigma(T)/dT approximately 0 as a function of

267 crossover from dsigma(T)/dT > 0 to dsigma(T)/dT approximately 0 as a function of the source-drain vol

268 rrent was the rate of change of temperature (dT/dt heat shock), not temperature itself.

269 t differ from those formed with a templating dT.

270 ntext, dA-rU base pairs are less stable than dT-rA base pairs.

271 We find that dT nucleotide flipping depends on DNA context and is eff

272 The dT preference of the ligase is interesting given the seq

273 In the template DNA-DNA duplex, the dT-dA base pairs are more stable than the corresponding

274 calated and a carcinogen-exposed SMI for the dT/-2 duplex.

275 Irradiation of the dT-1 and dC-1 cross-linked products at 254 nm leads to a

276 d lethal replication stress after thymidine (dT)-induced inhibition of DNP dCTP synthesis by switchin

277 horylase (hTP) is responsible for thymidine (dT) homeostasis, and its action promotes angiogenesis.

278 horylase (hTP) is responsible for thymidine (dT) homeostasis, promotes angiogenesis, and is involved

279 mutants growing in the absence of thymidine (dT) is preceded by a substantial resistance phase, durin

280 polymerase eta bypass may lead to M(1)dG to dT and frameshift but likely not M(1)dG to dA mutations

281 ding to base substitutions (mostly M(1)dG to dT and M(1)dG to dA) and frameshift mutations.

282 mutational pattern is consistent with dG to dT transversions in the repetitive guanine tracts.

283 9), whereas 17 was hydrolyzed exclusively to dT.

284 initiate unwinding of blunt-ended and twin (dT)(6)-tailed DNA reflect processes needed to engage the

285 olution of the Pf-SSB tetramer bound to two (dT)(35) molecules.

286 Removal of the wobble hydrogen bonds in U:dT recovers a strong response to methylene substitution

287 inding mode and between binding modes using (dT)(70) and (dT)(140).

288 ethylene substitution on the non-cognate UTP/dT scaffold ( approximately 3-fold decrease in kpol) is

289 tate for bond formation and cleavage for UTP/dT incorporation compared with ATP/dT incorporation.

290 roportion of -1 deletions were observed when dT was positioned 5' of M1dG.

291 pressure leads to the increase in T(c) with dT(c)/dP = +1.82 K.GPa(-1) while the resistance decrease

292 Pharmacological co-targeting of the DNP with dT and the NSP with DI-39 was efficacious against ALL mo

293 A much higher reactivity was observed with dT than dC or dA.

294 the highest aptamer signal was obtained with dT 11mers, with shorter aptamer linkers significantly re

295 ghest hybridization signal was obtained with dT 5mer linkers, and the highest aptamer signal was obta

296 ing patterns of the guanine when paired with dT or dA and suggest that N7 alkylation may alter the ba

297 m of N7mdG involves in its base pairing with dT.

298 ientations to form Hoogsteen base pairs with dT.

299 e dissociation constant for labeled SSB(2) x dT(70) to be 1.1 microM at a high ionic strength (200 mM

300 ctures of the 20 kDa apo-YdbC dimer and YdbC:dT(19)G(1) complex were determined.