ライフサイエンスコーパス: definitive endoderm

1 fects indicating that Hex is required in the definitive endoderm.

2 s not displaced proximally in the absence of definitive endoderm.

3 terior visceral endoderm and in the anterior definitive endoderm.

4 in interactions during differentiation into definitive endoderm.

5 cs during the first two divisions leading to definitive endoderm.

6 ropensity toward neuroectoderm and D2 toward definitive endoderm.

7 g embryonic and extra-embryonic mesoderm and definitive endoderm.

8 sential role for Nodal in development of the definitive endoderm.

9 e developmental ontology of the limb bud, or definitive endoderm.

10 nd gene expression pattern characteristic of definitive endoderm.

11 terning of mesoderm, and localization of the definitive endoderm.

12 he displacement of the primitive endoderm by definitive endoderm.

13 namely the node, notochord and the emerging definitive endoderm.

14 itant with the emergence of epiblast-derived definitive endoderm.

15 ning the embryonic axis and specification of definitive endoderm.

16 ic phenotypes are both due to defects in the definitive endoderm.

17 uch as prechordal plate, node, notochord and definitive endoderm.

18 tion of the AP axis and specification of the definitive endoderm.

19 s migrate out laterally to form mesoderm and definitive endoderm across the entire embryonic cylinder

20 a failure to correctly specify the anterior definitive endoderm (ADE) and prechordal plate (PCP) pro

21 a clear role for the hypoblast and anterior definitive endoderm (ADE) in patterning the overlying ec

22 nce of the forebrain, including the anterior definitive endoderm (ADE), anterior mesendoderm (AME) an

23 erm (AVE) and, subsequently, in the anterior definitive endoderm (ADE), anterior neuroectoderm (ANE),

24 thways required for gastrulation are active, definitive endoderm and all classes of mesoderm are spec

25 ing program differences are observed between definitive endoderm and cardiac mesoderm.

26 rotocols for rapid generation of nearly pure definitive endoderm and forebrain-patterned neural organ

27 The definitive endoderm and hepatic endoderm cells were char

28 Otherwise, lower Wnt signals lead to definitive endoderm and higher Wnt signals induce presom

29 tions indirectly in the survival of anterior definitive endoderm and in the maintenance of the anteri

30 gut spheroids, the intermediate step between definitive endoderm and mature organoids.

31 gel in promoting hPS cell differentiation to definitive endoderm and mesoderm.

32 eproducibly from mouse embryonic stem cells: definitive endoderm and neural organoids.

33 for GATA6 and GATA4 in the formation of both definitive endoderm and pancreatic progenitor cells.

34 nvolved in head patterning, such as anterior definitive endoderm and prechordal mesendoderm.

35 al foregut is derived from the most anterior definitive endoderm and that the liver is probably the m

36 ng gastrulation in the anterior visceral and definitive endoderm and the cephalic neural plate.

37 Helobdella embryo prevents the formation of definitive endoderm and the expression of Lox3 RNA and l

38 Prechordal plate, early definitive endoderm, and anterior visceral endoderm appe

39 ctly specify the anterior-posterior axis and definitive endoderm, and are viable and fertile.

40 ac haemogenic endothelium, cardiac mesoderm, definitive endoderm, and axial mesoderm progenitors duri

41 rt period of time as they contributed to the definitive endoderm, and combining this with in situ hyb

42 y specifying human pluripotent stem cells to definitive endoderm, anterior foregut endoderm, ventral

43 We find that definitive endoderm arises from lineage convergence: a d

44 ve endoderm lineage and will be displaced by definitive endoderm arising from the primitive streak du

45 marks the floor plate of the neural tube and definitive endoderm, as well as the node and notochord,

46 e both expressed prominently within anterior definitive endoderm at the time when removal of this tis

47 d 5 results in a permanent window denuded of definitive endoderm, beneath prechordal mesoderm and a v

48 This protocol first induces definitive endoderm by treatment with Activin A and CHIR

49 man embryonic stem cells (hESCs) and derived definitive endoderm, cardiac mesoderm, and ectoderm cell

50 The acceleration effects were observed in definitive endoderm, cardiomyocyte and neuronal differen

51 induction step allows for more efficient and definitive endoderm cell formation.

52 ed poor formation and abnormal allocation of definitive endoderm cells on embryonic day 7.5.

53 c stem cells and embryonic stem cell-derived definitive endoderm cells, screening 7905 distinct DNA s

54 um produced cultures consisting of up to 80% definitive endoderm cells.

55 Migrating cells (mesoderm and definitive endoderm) contain CXCR4 message while embryon

56 + /TBXT- embryonic disc cells directly form definitive endoderm, contrasting later-emerging FOXA2/TB

57 series of inductive interactions between the definitive endoderm (DE) and the surrounding splanchnic

58 K signalling antagonizes the Activin-induced definitive endoderm (DE) differentiation of human embryo

59 cale CRISPR screens to uncover regulators of definitive endoderm (DE) differentiation, which unexpect

60 culum (ER) stress and other stressors during definitive endoderm (DE) differentiation.

61 al endoderm (AVE), primitive streak (PS) and definitive endoderm (DE) have yet to be defined.

62 First, definitive endoderm (DE) is induced in the presence of h

63 nduced pluripotent stem cells stalled at the definitive endoderm (DE) stage.

64 We identify a definitive endoderm (DE) transcriptomic signature that l

65 begin with the differentiation of hPSCs into definitive endoderm (DE) using activin A, followed by th

66 ted differentiation of iPS and ES cells into definitive endoderm (DE) would allow the derivation of o

67 cts their differentiation efficiency towards definitive endoderm (DE).

68 ssed first in primitive endoderm and then in definitive endoderm derivatives, including glandular sto

69 traembryonic and precardiac mesoderm, and in definitive endoderm derivatives.

70 Definitive endoderm derived from mouse embryonic stem ce

71 anterior visceral endoderm and the anterior definitive endoderm, develop normally in nehe mutants.

72 ional inactivation of Lhx1 disrupts anterior definitive endoderm development and impedes node and mid

73 e functional copy of GATA6 is sufficient for definitive endoderm development and pancreas formation,

74 Sox17 is a key transcriptional regulator of definitive endoderm development, and yet, its genomic ta

75 Using human embryonic stem cell definitive endoderm differentiation as a dynamic transit

76 al enhancer for FAM189A2, thereby inhibiting definitive endoderm differentiation.

77 ed by a marked delay in the expansion of the definitive endoderm during gastrulation.

78 ta4 generates similar amounts of epCam+Dpp4- definitive endoderm enriched for Cxcr4, FoxA2, FoxA3, Dl

79 emonstrate that Hex function is essential in definitive endoderm for normal development of the forebr

80 ly rescues anterior-posterior patterning and definitive endoderm formation and results in adult viabi

81 The process of definitive endoderm formation in differentiating hES cel

82 rior movements associated with the epiblast, definitive endoderm formation, and anterior specificatio

83 type correlates with defects in mesoderm and definitive endoderm formation, as well as abnormal Nodal

84 This involved activin-induced definitive endoderm formation, FGF/Wnt-induced posterior

85 otein (BMP) signaling after specification of definitive endoderm from pluripotent cells results in a

86 olecular machinery that directs formation of definitive endoderm from pluripotent stem cells is not w

87 Previously we reported that definitive endoderm from which liver was derived, expres

88 by directing cells through stages resembling definitive endoderm, gut-tube endoderm, pancreatic endod

89 any regulatory networks involved in defining definitive endoderm have been identified, the mechanisms

90 ells are able to directly differentiate into definitive endoderm, hepatic progenitors, and mature hep

91 piblast stem cells to study specification of definitive endoderm in vitro.

92 (ES) cells to contribute descendants to the definitive endoderm in wild-type host embryos.

93 Similarly, in avians the underlying definitive endoderm induces cardiogenesis in precardiac

94 rectly positioned but development of rostral definitive endoderm is greatly disturbed in Hex(-/-) emb

95 of the later lulu embryo are also disrupted: definitive endoderm is specified but does not form a gut

96 combination of soluble molecules to generate definitive endoderm-like cells that did not pass through

97 e have focused on directing hES cells to the definitive endoderm lineage as this step is a prerequisi

98 development, but are not recruited into the definitive endoderm lineage during gastrulation.

99 thways are required for specification of the definitive endoderm lineage in mammals and identify Smad

100 expression in the epiblast marks the entire definitive endoderm lineage, the anterior mesendoderm, a

101 drive stem cell differentiation towards the definitive endoderm lineage.

102 t Eomes is required for specification of the definitive endoderm lineage.

103 Conversely, they were negative for a definitive endoderm marker (Sox17) and did not generate

104 x1 target genes, including numerous anterior definitive endoderm markers and components of the Wnt si

105 embryos display defects in headfold fusion, definitive endoderm migration and a failure of the later

106 It is expressed in the definitive endoderm, notochord, and neural tube in embry

107 anterior visceral endoderm (AVE) and rostral definitive endoderm of early mouse embryos.

108 Morphometric analysis revealed that the definitive endoderm of mouse wild-type embryos undergoes

109 to the primitive streak, and subsequently to definitive endoderm of the area pellucida.

110 he visceral endoderm of the yolk sac and the definitive endoderm of the embryo.

111 ferentiation of hESC cultures toward neural, definitive endoderm/pancreatic and early cardiac muscle

112 HDE1 marks a definitive endoderm population with high hepatic potenti

113 ne microenvironments codifying hPSCs towards definitive endoderm, precardiac or presomitic mesoderm w

114 ibiting glycogen synthase kinase 3 (GSK3) on definitive endoderm production.

115 fibroblasts as early as 2.5 weeks; and human definitive endoderm progenitors can be differentiated in

116 2-5 weeks; expandable neural stem cells and definitive endoderm progenitors can be obtained from hum

117 etween stages 3a/b and 4, the intraembryonic definitive endoderm receives contributions mainly from t

118 directed differentiation results in abnormal definitive endoderm specification in mouse and human, ch

119 r of anteroposterior axis formation, EMT and definitive endoderm specification in the mouse.

120 atial, molecular, and temporal events during definitive endoderm specification.

121 and stage specific, with many primed at the definitive endoderm stage.

122 Of interest are organs derived from the definitive endoderm, such as the pancreas and liver, and

123 Of great interest are organs derived from definitive endoderm, such as the pancreas.

124 ventral region of mutant embryos within the definitive endoderm, suggesting an important role of thi

125 in the mutants in several tissues including definitive endoderm, suggesting that a deficiency in ves

126 factor, Sox17, is required for formation of definitive endoderm that gives rise to various organs, i

127 Anterior definitive endoderm, the future pharynx and foregut lini

128 suggest Otx2 as a candidate regulator of the definitive endoderm, the precursor of all gut-derived or

129 required to specify hepatic fate within the definitive endoderm through activation of the FGF recept

130 at the primitive streak to form mesoderm and definitive endoderm, through an epithelial-mesenchymal t

131 then displaced away from the epiblast by the definitive endoderm to become associated with the extra-

132 bsequently, Hex is expressed in the earliest definitive endoderm to emerge from the streak and its ex

133 duction and anterior-posterior patterning of definitive endoderm to generate a coherent roadmap for e

134 We also show that overexpression of two definitive endoderm transcription factors, T and Foxa2,

135 e relationship between the hypoblast and the definitive endoderm was defined by following labeled ros

136 Sox17 is required for Nepn expression in the definitive endoderm, while RA signaling restricts expres

137 es against human embryonic stem cell-derived definitive endoderm with the goal of identifying cell su