ライフサイエンスコーパス: deglycosylated

1 hERG protein accumulated in the cytosol was deglycosylated.

2 Deglycosylated [(89)Zr]Zr-DFO-alpha-miSOD1 results in hi

3 ith an excess of alpha-miSOD1 or imaged with deglycosylated [(89)Zr]Zr-DFO-alpha-miSOD1 to assess tar

4 2D)beta2gamma2S receptors, endoglycosidase H deglycosylated 91 +/- 4% of alpha1(A322D) subunits, cons

5 In particular, ITs constructed with the deglycosylated A chain prepared from native ricin were t

6 together with estimation of the size of the deglycosylated A subunit relative to a series of C-termi

7 ase or decrease in C3b deposition on the two deglycosylated Abs was magnified when complement activat

8 irst glycosylated in the ER and subsequently deglycosylated after retrotranslocation into the cytosol

9 ition experiments conducted using native and deglycosylated aggrecan provided direct evidence for int

10 Apoptolidin H, the C27 deglycosylated analogue of apoptolidin A, displayed sub-

11 ased on the experimental retention values of deglycosylated analogues (HI(glyco) = HI(deglyco) + dHI,

12 ed for 602 glycopeptides versus 123 of their deglycosylated analogues.

13 red with the hydrophobicity indices of their deglycosylated analogues.

14 tructures of a PME from Aspergillus niger in deglycosylated and Asn-linked N-acetylglucosamine-stub f

15 Juniperus ashei pollen extract was deglycosylated and characterised using SDS-PAGE and immu

16 ELISAs with deglycosylated and desialated Spike S1 subunits revealed

17 vity for glucose oxidation compared with the deglycosylated and glycosylated (gPDH) forms of PDH.

18 dem mass spectrometry (LC/MS/MS) analysis of deglycosylated and intact glycopeptides from tryptic dig

19 NGaseF, and RPLC-MS/MS analysis of combined (deglycosylated and intact) peptide mixtures.

20 e wild type US2 and US11 proteins as well as deglycosylated and polyubiquitinated class I degradation

21 a dimeric oxidized complex and then becomes deglycosylated and reduced to monomers.

22 d from the ER to the cytosol, where they are deglycosylated and subsequently degraded by the proteaso

23 sgenic mouse hearts produced both the 35-kDa deglycosylated and the 40-kDa glycosylated mobility form

24 s were observed with both intact gp120 and a deglycosylated and truncated form of gp120 protein that

25 ze the LC binding site on THP, human THP was deglycosylated and underwent limited trypsin digestion i

26 rst generated hypersialylated, desialylated, deglycosylated and untreated serum IgG and found that th

27 To prolong avidin clearance, avidin was deglycosylated and/or neutralized by acetylation of its

28 14 is endocytosed, extracted from membranes, deglycosylated, and degraded by proteasomes.

29 om the endoplasmic reticulum to the cytosol, deglycosylated, and degraded by the proteasome.

30 polonica converted stilbenes to ring-opened, deglycosylated, and dimeric products.

31 Our data for glycosylated, partially deglycosylated, and fully deglycosylated molecules furth

32 Ephrin-A1 was enzymatically deglycosylated, and its activity was evaluated in severa

33 SO-treated, and the protein was pulled down, deglycosylated, and resolved by electrophoresis.

34 ic mice receiving either anti-Abeta (2H6) or deglycosylated anti-Abeta (de-2H6) showed significant im

35 Deglycosylated anti-Abeta antibodies may be preferable t

36 Humanized, deglycosylated anti-HPA-1a mAbs may represent a novel tr

37 tilage (without additional purification), if deglycosylated appropriately, can be used as arthritogen

38 Previous studies suggested that deglycosylated beta1-LAP can not form this latent comple

39 s, and PS.beta2GPI binding experiments using deglycosylated beta2GPI, suggested that carbohydrate res

40 experiments conducted with an enzymatically deglycosylated Bla g 2 and a N268Q mutant showed that th

41 A deglycosylated breakdown intermediate of the heavy chain

42 In vitro phosphorylation of native and deglycosylated BSPs by casein kinase II identified seven

43 During this process, the Class I MHC HCs are deglycosylated by an N-glycanase-type activity.

44 lycans, or membrane-associated and partially deglycosylated by cytosolic N-glycanase.

45 rprised to find both molecules that had been deglycosylated by peptide N-glycanase and a large number

46 Glycopeptides thus selected were mixed, deglycosylated by PNGase F, and fractionated by reversed

47 N-deglycosylated C1INH also failed to suppress fluorescein

48 an enzyme linked immunosorbent assay, the N-deglycosylated C1INH bound to LPS very poorly.

49 veral in vitro and in vivo models, whereas N-deglycosylated C1INH loses such activities.

50 As with intact LPS, binding of N-deglycosylated C1INH to dLPA and mLPA was diminished in

51 We analyzed both native and enzymatically deglycosylated C4b2 and C2 and showed that the resulting

52 imilar at 4.1 to 4.2 nm for glycosylated and deglycosylated C5-Fc.

53 Purified deglycosylated CD147 failed to induce MMP-1 or MMP-2, bu

54 Difference maps between the glycosylated and deglycosylated CD155 complexes determined the sites of t

55 Both glycosylated and fully deglycosylated CD155 exhibited similar binding sites and

56 etry on purified and peptide N-glycosidase F-deglycosylated CD36 and also by comparing the electropho

57 enhanced binding; (c) O-linked glycans on N-deglycosylated CD44 enhanced binding; and (d) N-acetylga

58 Our data show that glycoforms of CD66e and deglycosylated CD66e are recognized by gonococci in an O

59 Analysis of deglycosylated cell lysates indicated that up to 40 kDa

60 g deglycosylation of cell membrane proteins (deglycosylated cells Bmax: 6.83 Hz; glycosylated cells B

61 Both the level and stability of cytosolic, deglycosylated CFTR are increased by proteasome blockade

62 (4 microM, 1 h) stabilizes the presence of a deglycosylated CFTR intermediate for up to 5 h without i

63 Deglycosylated CFTR is present under the same conditions

64 The mass differences between the deglycosylated chain a (17, 524.0) and a1 to a4 correspo

65 nt and the single-channel conductance of the deglycosylated channels was slightly reduced relative to

66 Deglycosylated channels were significantly more active (

67 spectral differences between the native and deglycosylated channels, thereby indicating that no net

68 differences in stability between native and deglycosylated channels.

69 olated, purified by gradient centrifugation, deglycosylated, characterized, and tested for arthritis

70 he 2.9-A-resolution structure of a partially deglycosylated complex of human FSH bound to the extrace

71 n at their predicted molecular masses in the deglycosylated complex.

72 the existence of several naturally occurring deglycosylated components of DLIF in human serum.

73 The presence of deglycosylated congeners of DLIF in human serum, includi

74 ortion of these molecules shows DLIF and its deglycosylated congeners to be 10(3)-fold less reactive

75 beled that molecule within its M(r) = 45,000 deglycosylated core.

76 roversial folded-back crystal structures for deglycosylated CR2 SCR 1-2.

77 he integrity of the protein, as truncated or deglycosylated Cripto-1 lacked BMP-4 binding activity.

78 in are upregulated after SCI and degrade the deglycosylated CSPG proteins.

79 Proteasome inhibitors can yield deglycosylated cytoplasmic intermediates that are the re

80 esized (AO)7 and anhydrous hydrogen fluoride-deglycosylated d(AO)51.

81 ) acceptor and a transgenically produced and deglycosylated d[AO](51) acceptor.

82 Oleuropein and its deglycosylated derivatives have high pharmaceutical inte

83 tion of the existence of naturally occurring deglycosylated derivatives of DLIF and establishes the l

84 subunit preparations and their alphaL2 loop-deglycosylated derivatives were combined with either eLH

85 uantified in all samples and might have been deglycosylated during digestion, increasing the bioacces

86 to-apyrase, in contrast to the enzymatically deglycosylated ecto-apyrase and the tunicamycin-treated

87 c SIVmac239 and a live-attenuated, quintuple deglycosylated Env mutant (Delta5G) virus target CD4(+)

88 mented deglycosylated PDH is formed from the deglycosylated enzyme (dgPDH) when it spontaneously lose

89 ized glucose-oxidizing anode, prepared using deglycosylated enzyme coimmobilized with [Os(4,4'-dimeth

90 All the glycosylated and deglycosylated enzymes show direct electron transfer (DE

91 d by surface plasmon resonance confirms that deglycosylated ephrin-A1 does not bind EphA2 with high a

92 We found that deglycosylated ephrin-A1 does not efficiently induce Eph

93 doglycosidase-catalyzed glycosylation of the deglycosylated Fc domain (GlcNAc-Fc homodimer) with the

94 ce reducing CGE based on its tracking of the deglycosylated Fc species.

95 Similar studies of partially deglycosylated FH suggested that oligosaccharides were n

96 ndoplasmic reticulum and can be recovered as deglycosylated fluorescent intermediates in the cytosol.

97 Moreover, the deglycosylated form of both wild type and DeltaF508 CFTR

98 s in complex with an enzymatically partially deglycosylated form of the 3-domain ectodomain of Pvr at

99 tained from enzyme electrodes containing the deglycosylated form of the enzyme.

100 cells comigrates with both the native and a deglycosylated form of the radiolabeled receptor.

101 treated or pretreated with gp120 or with the deglycosylated form of this protein.

102 this study, we describe an endoglycosidase H-deglycosylated form of TPP1 containing four Asn-linked N

103 pastoris and at 1.40 A for the mutagenically deglycosylated form produced from Sf9 cells, show that t

104 The recombinant enzyme and a deglycosylated form, both expressed in Pichia pastoris,

105 coprotein coagulation factor XII and not its deglycosylated form, but the mechanism for this specific

106 injection of soluble egg Ag, but not by its deglycosylated form, suggesting a role for carbohydrates

107 itical since gangliosides are unable to bind deglycosylated forms of alpha(5)beta(1) from SCC12 and i

108 netic studies with native, desialylated, and deglycosylated forms of both enzymes indicate that the m

109 ized the detection and quantification of the deglycosylated forms of full-length PrP(C) and its C-ter

110 ity and recognized the glycosylated and both deglycosylated forms of gamma-glutamyl hydrolase.

111 zes the gI protein directly since completely deglycosylated forms of gI are efficiently recognized.

112 comparing exchange into the glycosylated and deglycosylated forms of the antibody.

113 tricted epitope, but not the glycosylated or deglycosylated forms.

114 insect cells) < pTF for the glycosylated and deglycosylated forms.

115 model predicts that FSHR binds Asnalpha(52)-deglycosylated FSH at a 3-fold higher capacity than full

116 rs, the binding of glycosylated FSH, but not deglycosylated FSH, would increase 3-fold, and that the

117 yses interaction showed decreased binding of deglycosylated full-length CFH to immobilized C3b, showi

118 We also show that deglycosylated, full-length allergen is not activated ef

119 M) and that it did not bind to enzymatically deglycosylated gB2.

120 Deglycosylated Gc protein cannot be converted to macroph

121 Deglycosylated glycodelin was equipotent to intact glyco

122 ecific monoclonal antibodies with intact and deglycosylated glycoproteins, hemocyte extracts, and int

123 One PEG variant was created by modifying deglycosylated GO with short PEG chains (average of 350

124 udies have focused on the heavily truncated, deglycosylated gp120 core, leaving fundamental aspects o

125 lved structure of a mutant truncated, highly deglycosylated gp120 to propose a structural model for t

126 Pretreatment of CNS cultures with deglycosylated gp120, anti-T4A or anti-GC antibodies did

127 was displaced by unlabeled gp120 but not by deglycosylated gp120, suggesting that the binding was sp

128 the crystal structure of CD4 with truncated deglycosylated gp120.

129 omplexes were also produced of enzymatically deglycosylated gp160e and of gp160e variants with deleti

130 ered a strategic thrombin cleavage site into deglycosylated, HA-tagged human VMAT2.

131 - 2.0 Da) are isoforms of L1 (25, 837.5 in N-deglycosylated Hb) with glycan side-chains (GlcNAc)2 (Ma

132 with Au-hCG, LH receptors labeled with gold-deglycosylated hCG, an hCG antagonist, also exhibit rest

133 Recombinant and deglycosylated HCII bound heparin with dissociation cons

134 Binding of recombinant and deglycosylated HCII to DS, both with K(D) 4+/-1 microM,

135 for thrombin inactivation by recombinant and deglycosylated HCII were comparable, at optimal GAG conc

136 ound that compared with native hemocyanins N-deglycosylated hemocyanins elicited reduced antibody tit

137 Evaluating the function of N-deglycosylated hemocyanins in the humoral immune respons

138 al analyses indicated decreased binding of N-deglycosylated hemocyanins to the MR and MGL receptors a

139 gG(1-4)), and recognize hIgG(1-4) as well as deglycosylated hIgG in western blotting assays.

140 recombinantly expressed human OGA, yielding deglycosylated histone H3 peptides.

141 46 kDa), similar to that of enzymatically N-deglycosylated hNET protein, which confirms the use of c

142 species, HO2-Co(III)-PEP (denoted CoPEP) and deglycosylated HO2-Co(III)-PEP (denoted CodPEP) have bee

143 these issues, we subjected glycosylated and deglycosylated human myeloma IgG3 to multidisciplinary s

144 we report the three-dimensional structure of deglycosylated, human erythrocyte AQP1, determined at 7

145 sent a three-dimensional (3D) density map of deglycosylated, human erythrocyte aquaporin 1 (AQP1) det

146 cR recognition, the receptor affinities of a deglycosylated IgG1 and its Fc fragment were determined

147 ntaining 135,135 and 73,905 glycosylated and deglycosylated IgG3 structures, respectively.

148 of 5.82 to 6.29 S for both glycosylated and deglycosylated IgG3.

149 n the impaired in vitro FcgammaRI binding of deglycosylated immunoconjugates and significant decrease

150 complex mixture from human plasma were fully deglycosylated in 20 min, without any apparent adverse a

151 glycosylated SKN-1A/Nrf1 exits the ER and is deglycosylated in the cytosol by the PNG-1/NGLY1 peptide

152 om corresponding glycan oxazolines to the Fc-deglycosylated intact IgGs without product hydrolysis.

153 asome-inhibited US11 cells demonstrated that deglycosylated intermediates continued to be integrated

154 b ions and is supported by data acquired for deglycosylated KiH bsAb and mAb constructs, each of whic

155 pecific immunoreactivity of glycosylated and deglycosylated LeAGP-1 with an antibody developed agains

156 Unexpectedly, these deglycosylated Lfs exhibited no loss in their ability to

157 dditionally, in cells, calreticulin binds to deglycosylated major histocompatibility complex class I

158 In addition, the deglycosylated MAL-bound proteins still interact with Om

159 N-linked carbohydrates and had an estimated deglycosylated mass of 64,000 Da.

160 correlated with enhanced calnexin binding to deglycosylated MHC class I heavy chains.

161 hogenic IgGs bound recombinant mouse MOG and deglycosylated MOG in myelin (tested by Western blot), b

162 sylated, partially deglycosylated, and fully deglycosylated molecules further revealed the criticalit

163 mulate in the cytosol as already reduced and deglycosylated molecules.

164 genic SIVmac239 virus and a live-attenuated, deglycosylated mutant Delta5G virus infected distinct CD

165 on their surfaces, the SF162-derived V2 loop-deglycosylated mutant viruses replicate as efficiently a

166 A deglycosylated mutant, beta(2-DeltaN), had no effect on

167 Molecular mass determinations of deglycosylated mZP2 and mZP3 suggest that their polypept

168 on differential HX-MS measurements, while 5% deglycosylated NIST mAb was not statistically equivalent

169 Both of the deglycosylated, nonreduced HA receptor proteins still bi

170 ha1beta2gamma2S receptors, endoglycosidase H deglycosylated only 26 +/- 5% of alpha1 subunits, consis

171 ntly inhibited if the antigen was chemically deglycosylated or if mannose receptors were blocked with

172 Increased levels of deglycosylated or newly formed metabolites such as kaemp

173 7 (77%) had IgE to both the glycosylated and deglycosylated Ory c 3 heterodimer.

174 Graphite electrodes modified with deglycosylated PcCDH (dPcCDH) and CsCDH (dCsCDH) have a

175 Fragmented deglycosylated PDH is formed from the deglycosylated enz

176 Moreover, deglycosylated PDLIM7 significantly up-regulated the act

177 We found that the aberrantly deglycosylated PDLIM7 suppressed p53 gene expression and

178 d except proline) motif were identified from deglycosylated peptide data as potentially atypical glyc

179 The deglycosylated peptide fractions were interrogated by ES

180 For grape invertase, in parallel with deglycosylated peptides analysis, different enzymatic di

181 ermore, our approach enabled the analysis of deglycosylated peptides and glycans from enriched IGPs f

182 y (MS)-based glycoproteomic approach wherein deglycosylated peptides are analysed by LC-MS/MS, while

183 ion that glycopeptides and the corresponding deglycosylated peptides share very similar MS/MS pattern

184 ternal standard technology, fractionation of deglycosylated peptides with reversed-phase chromatograp

185 alpha1-acid glycoprotein, the corresponding deglycosylated peptides, and the glycans released by the

186 ms of individual N-linked glycopeptides, the deglycosylated peptides, and the released, deutero-reduc

187 e that full length, Endo H in vivo enzymatic deglycosylated Pfs48/45 antigen is produced at a high le

188 Thus, Endo H in vivo enzymatic deglycosylated Pfs48/45 is a promising candidate for the

189 Dephosphorylation of deglycosylated PHF tangles results in increased tau rele

190 ed that recognition was mediated only by the deglycosylated phosphoantigen.

191 K-293/C-21 cells) was similar to that of the deglycosylated photo-cross-linked receptor (obtained by

192 tivities of recombinant HCII, and native and deglycosylated plasma HCII, and their affinities for hep

193 Peanut Ag (PNAg), but not deglycosylated PNAg, activated monocyte-derived dendriti

194 From these digests the completely deglycosylated polypeptide corresponding to RNase A in w

195 The crystal structure of deglycosylated pro-TPP1 was determined at 1.85 angstroms

196 of glycopeptides and glycoproteins and their deglycosylated products.

197 termined at 1.85 A for the endoglycosidase H-deglycosylated protease domain produced from P. pastoris

198 The expressed deglycosylated protein is the same size as SIRPalpha1, b

199 ha, the yield, purity and homogeneity of the deglycosylated protein resulting from this expression sy

200 t the OGA stalk domain mutant preferentially deglycosylated protein substrates with +2 proline in the

201 N-Glycans, deglycosylated proteins, and intact glycoproteins from h

202 followed by separate analysis of glycans and deglycosylated proteins, which limits the ability to der

203 hout one of the carbohydrate chains, and the deglycosylated proteins-are detected by ProteinChip arra

204 tablished that mHR23B acts as a receptor for deglycosylated proteins.

205 o their enzymatic properties and mass of the deglycosylated proteins.

206 lar dynamics simulations of glycosylated and deglycosylated proteins.

207 n of calreticulin contributes to binding non/deglycosylated proteins.

208 ified PMCAb conditions that employ partially deglycosylated PrP(C) (dgPMCAb), an alternative transmis

209 mes and the sizes of the protease-resistant, deglycosylated PrP(Sc) fragments.

210 al rabbit antibodies were raised against the deglycosylated purified protein (dPELP) and purified ant

211 was expressed inPichia pastoris.Mo-MnLOX was deglycosylated, purified to homogeneity, and subjected t

212 The present study focuses on fragmented deglycosylated pyranose dehydrogenase (fdgPDH) from Agar

213 orylation, detected by the mobility shift of deglycosylated receptor, was unaffected by beta-arrestin

214 ple bands between 50 and 75 kDa, whereas the deglycosylated receptors migrated at 32 kDa, correspondi

215 lipoprotein, when comparing glycosylated and deglycosylated receptors.

216 HMW-BNP, when deglycosylated, was similar to deglycosylated recombinant proBNP.

217 With time, N-cadherin in clone-YH becomes deglycosylated, resistant to detergent extraction, and z

218 We also generated enzymatically deglycosylated rhGAA that was shown to be partially acti

219 It allowed us to crystallize a fully deglycosylated rhodopsin (N2C/N15D/D282C).

220 IgG-HD37-SMPT-dgA is a deglycosylated ricin A chain (dgA)-containing immunotoxi

221 l antibody SN7 (IgG1) and its conjugate with deglycosylated ricin A chain (dgRA).

222 MoAb) has been linked chemically with either deglycosylated ricin A chain or truncated versions of Ps

223 ICs containing deglycosylated ricin A chain prepared from ricin toxin e

224 degraded to any significant extent; however, deglycosylated S(3)-RNase is degraded as efficiently as

225 The deglycosylated sample was further fractionated by revers

226 When the AGPs were partly or fully deglycosylated, SE-promoting activity was not diminished

227 lar dichroic spectra of the glycosylated and deglycosylated (Ser-Pro(2))(n), (Ser-Pro(3))(n), and (Se

228 -treated SP-A still enhanced uptake, whereas deglycosylated SP-A had significantly diminished ability

229 Furthermore, incubation of deglycosylated SP-A with M. tuberculosis failed to suppr

230 y recognizes LeAGP-1 in its glycosylated and deglycosylated states, and (4) immunolocalization of a s

231 C5-Fc were removed and the glycosylated and deglycosylated structures were evaluated.

232 ature TC II-R (62 kDa) and the enzymatically deglycosylated TC II-R (45-47 kDa) demonstrated optimal

233 de ((1)H(6)/(2)D(6)) reagents, enzymatically deglycosylated the differentially labeled peptides in th

234 were able to be reduced to a 46-kDa form and deglycosylated to a 38-kDa protein.

235 MS/MS experiment, so the tryptic digest was deglycosylated to confirm the presence of the glycopepti

236 inal subcellular compartments, where Nrf1 is deglycosylated to yield an active 95-kDa isoform.

237 The partially deglycosylated trimers are stable, and neither abnormall

238 on microscopic analysis shows that partially deglycosylated trimers have a structure similar to fully

239 Moreover, the deglycosylated trimers retain or increase their ability

240 Two unique deglycosylated tryptic peptides, (21)EAENITTGCAEHCSLNENI

241 lated and ubiquitinated species as well as a deglycosylated US11 intermediate.

242 y constant for the binding of neutravidin, a deglycosylated variant of avidin, to surface-immobilized

243 ino acid BNP standard, whereas HMW-BNP, when deglycosylated, was similar to deglycosylated recombinan

244 vative Hbs (reduced, carbamidomethylated and deglycosylated) were analysed by electrospray ionization

245 vative Hb (reduced, carbamidomethylated, and deglycosylated) were analyzed by electrospray ionization

246 egrees C for 90min and could then be further deglycosylated when mixed with almond or flax seed.

247 1 interacts with the O-GlcNAcase and becomes deglycosylated, which is likely to be important for expo

248 neously glycosylated IgG N-glycans are first deglycosylated with a wild-type endoglycosidase.

249 NPR-ECD deglycosylated with endoglycosidase F2 and endoglycosida

250 LAP produced in Chinese hamster ovary cells, deglycosylated with N-glycosidase F, forms a 80-90 kDa c

251 The purified enzyme could be deglycosylated with peptide N-glycosidase-F to a core mo

252 were differentially labeled, mixed, and then deglycosylated with the enzyme PNGase F.

253 esized protein into the cytosol, where it is deglycosylated, with conversion of the asparagines to as