ライフサイエンスコーパス: deletional

1 ong- standing model that U-insertional and U-deletional activities are separated within the editing c

2 RBCs are distinctly overhydrated relative to deletional alpha-thalassemia variants, and the derangeme

3 erations we analyzed membranes from HbCS and deletional alpha-thalassemic variants and found that in

4 Over three decades, transgenic and deletional analyses have demonstrated both the necessity

5 Deletional analyses localized the residues comprising th

6 Deletional analyses of hVps35 demonstrate that amino aci

7 Deletional analyses of the Npr1 promoter along with ecto

8 Deletional analyses of the proximal promoter and the ent

9 Deletional analyses reveal that for each element multipl

10 Progressive deletional analyses together with site-specific mutagene

11 Deletional analyses using heterologous promoter construc

12 o characterize the Xic further, we performed deletional analysis across the 450-kb region by yeast-ar

13 Deletional analysis also suggested that the entire C-ter

14 Reporter gene deletional analysis and EMSAs indicate an IL-2-regulated

15 Deletional analysis and reporter expression assays ident

16 y dependent on the C1 domain, as revealed by deletional analysis and site-directed mutagenesis.

17 An OSM response element was mapped by deletional analysis between base pairs (bp) -148 and -10

18 Deletional analysis carried out in the absence of the c-

19 Deletional analysis confirmed that some of these novel s

20 Deletional analysis confirmed that specific sequences wi

21 Additionally, deletional analysis demonstrated a significant IS contri

22 A comparative deletional analysis demonstrated that a shorter region e

23 The results of the deletional analysis demonstrated that constructs capable

24 Deletional analysis demonstrated that residues present w

25 Deletional analysis demonstrated that the -1098 to -93 r

26 A comprehensive deletional analysis established two regions rich in Sp1

27 tween the two proteins were characterized by deletional analysis in both yeast two-hybrid and gel shi

28 Deletional analysis in human CETP transgenic mice locali

29 Deletional analysis localized a major regulatory region

30 Deletional analysis localized the mSin3A-interacting dom

31 We have performed a detailed deletional analysis of DNA ligase IV to define its XRCC4

32 Deletional analysis of human pIgR revealed that the lack

33 ized to endosomal and vacuolar membranes and deletional analysis of Pib1p domains indicated that loca

34 Data from comparative structural and deletional analysis of PTB are consistent with the prese

35 Initial deletional analysis of the CbpA protein in strain D39 (c

36 Deletional analysis of the EMX2 5' regulatory region rev

37 Deletional analysis of the enhancer identified several s

38 Deletional analysis of the hAhR transactivation domain i

39 Deletional analysis of the human homologue of Skn-1a wit

40 To identify the minimal sequence, a deletional analysis of the late region was performed by

41 Deletional analysis of the murine 5'-flanking region rev

42 Deletional analysis of the promoter indicated that a pro

43 5' deletional analysis of the promoter indicated that the r

44 Deletional analysis of the Q-rich subdomain identified a

45 Fine deletional analysis of this region revealed that a four-

46 Deletional analysis revealed several consensus sequences

47 Deletional analysis revealed that a minimal sequence of

48 Finally, bidirectional deletional analysis revealed that multiple elements are

49 A deletional analysis revealed that the C1 domain in chima

50 Deletional analysis reveals that an AP-1-binding site (5

51 Further deletional analysis showed that a 220-bp region of the h

52 Deletional analysis showed that ADA3 directly associates

53 Additional deletional analysis showed that the SH3 domain was not r

54 In addition, deletional analysis suggested that a core 91-residue seq

55 We demonstrated by deletional analysis that Rad-50/Mre-11 and Ku86 were rec

56 Deletional analysis using epitope-tagged VGF suggested t

57 Deletional analysis was used to generate a beta-catenin

58 582 and amino acids 775-826) were defined by deletional analysis, each of which could act independent

59 Deletional analysis, using both in vitro protocols and c

60 Using deletional analysis, we confirmed that inhibitory activi

61 Using deletional analysis, we have identified in the carboxyl-

62 determine the CbpA-binding motif on pIgR by deletional analysis.

63 Further deletional and alanine mutagenesis analyses and comparis

64 In the present study, we have utilized deletional and alanine substitution mutagenesis within t

65 y NT by using fibroblasts that had undergone deletional and crossover/gene conversion events, respect

66 Deletional and GAL4-fusion gene analyses now reveal repr

67 In this model, both deletional and immunoregulatory mechanisms are active du

68 gene segments across a 3.2 Mb region in both deletional and inversional orientations.

69 alleles remain partially functional for both deletional and inversional recombination and can hence e

70 Deletional and mutational analyses identified that Plk1

71 Deletional and mutational analyses indicated that the pu

72 ranscriptional activator for CYP7A1, we used deletional and mutational analyses of CYP7A1 promoter se

73 Using deletional and mutational analysis of the cyclin A promo

74 Deletional and mutational studies revealed that C/EBP bi

75 Based on deletional and mutational studies, the sequence selectiv

76 s and cells isolated from patients with both deletional and nondeletional HbH disease, demonstrating

77 elopment of immunological tolerance via both deletional and regulatory mechanisms further adds to thi

78 To address this issue, we used deletional and site-directed mutagenesis approaches to s

79 Deletional and site-directed mutagenesis localized the h

80 Deletional and site-directed mutagenesis revealed the pr

81 In this study, we used deletional and site-directed mutagenesis to identify por

82 the activity of RpCAT in response to Rta by deletional and site-directed mutagenesis.

83 tes in the M(3)-MR i3 loop via a strategy of deletional and site-directed mutagenesis.

84 l basis of their heterotypic interactions by deletional and site-specific mutagenesis.

85 Serial deletional and targeted mutational analysis of cis-eleme

86 TbMP18 is critical for the U-deletional and U-insertional cleavages and for integrity

87 Both U-deletional and U-insertional cleavages are reasonably ac

88 kappa joining, RAG utilizes Vkappa-RSSs from deletional- and inversional-oriented clusters(8), incons

89 anism of inhibition was not dependent upon a deletional, apoptotic pathway, but it was reversed by an

90 es in gamma-globin levels seen in some human deletional beta-thalassemias and hereditary persistence

91 Here we discuss the evidence showing that deletional bias is a major force that shapes bacterial g

92 on--above a maximum threshold--will engage a deletional checkpoint for removal of self-reactive B cel

93 e-B cell receptor signaling, which engaged a deletional checkpoint for the removal of autoreactive B

94 y interconverted editing sites reveal that U-deletional cleavage is inefficient without and activated

95 Analysis of various 3'alpha E deletional constructs indicates that sequences flanking

96 By utilizing a series of mutational and deletional constructs of LDLR promoter luciferase report

97 Transient transfection assays of a series of deletional constructs show that the first hNET intron co

98 Deletional constructs were expressed in IMR32 cells.

99 er, the mechanism by which 53BP1 facilitates deletional CSR and inhibits inversional switching events

100 ated S regions routinely participate in such deletional CSR joining(11).

101 ion transcription, CSR center formation, and deletional CSR joining.

102 an acceptor S-region-downstream DSB-end for deletional CSR(12).

103 1) by which IgH organization in cis promotes deletional CSR.

104 ation for the link with 53BP1 enforcement of deletional CSR.

105 lly align a downstream S region with Smu for deletional CSR.

106 itiated donor and acceptor S-region DSBs for deletional CSR.

107 Interestingly, the thymic deletional effect of sHEL was superior to that of mHEL i

108 Ku80-dependent NHEJ repair, thus preventing deletional end-joining of chromosomal DSBs during G1.

109 J-mediated DSB repair and prevents mutagenic deletional end-joining through interaction with canonica

110 egions and functions via an intrachromosomal deletional event between the donor Smicro region and a d

111 ne segments and orchestrates their fusion as deletional events that assemble a V(D)J exon in the same

112 rearrangement generates more diversity than deletional events, and (c) fine differences exist in the

113 Through deletional experiments, we showed that the inhibitor of

114 Mice deletional for either MCP-1 or CCR2 had suppressed cell-

115 clude that Th2 cells do not undergo an early deletional form of tolerance after PIT.

116 These deletional forms of HPFH are poorly understood and are t

117 rames, implicated in the HPFH-1/2 and HPFH-6 deletional forms of the human syndrome, hereditary persi

118 A series of deletional fusion proteins of triadin 1 was generated, a

119 Among the patients with deletional hemoglobin H disease, 15% had one or both par

120 Both noncrossover and deletional homologous repair products are detected, at a

121 carrying an exact beta locus replicate of a deletional HPFH mutation, HPFH 2.

122 n (Apc(Min/+)) mouse model, as mutational or deletional inactivation of APC is a frequent early event

123 Deletional inactivation of the gene encoding d-serine de

124 With deletional, insertional and alanine-scanning mutagenesis

125 T cells transit through a CD8lowV beta 5low deletional intermediate during tolerance induction by a

126 5+ T cells transit through a CD8lowVbeta5low deletional intermediate during tolerance induction.

127 Using multiple deletional isoforms of PARP-1, lacking one or another of

128 y oriented D-12RSSs and, thereby, to mediate deletional joining of all D segments except RC-based DQ5

129 wnstream and upstream by 12RSSs that mediate deletional joining with convergently oriented J(H)-23RSS

130 Transient transfection studies using deletional M-CSF promoter constructs demonstrated that T

131 is of these results, we performed a detailed deletional map and identified two distinct regions of de

132 A refined deletional map for 15 VH gene families and an individual

133 ontaining this gene, we performed fine-scale deletional mapping of chromosome 6q in 22 acute/lymphoma

134 a putative tumor suppressor gene may reside, deletional mapping was performed using 33 microsatellite

135 o each other in vitro, and using progressive deletional mapping, we further uncovered the required do

136 bodies were detectable, suggesting a central deletional mechanism for tolerance.

137 ic tolerance is due mainly to an intrathymic deletional mechanism in these mixed chimeras.

138 Consistent with a requirement for a deletional mechanism in this IL-2Rbeta(-/-) model, a sma

139 e of single-strand annealing, an error-prone deletional mechanism of DSB repair.

140 ance is achieved primarily through a central deletional mechanism when peripheral and intrathymic hos

141 fore, the loss of one copy of PU.1 through a deletional mechanism, plus down-regulation of the residu

142 n of an alloreactive T-cell population via a deletional mechanism.

143 self-reactive T cells that have escaped the deletional mechanisms of central tolerance.

144 e receiving BALB/c hearts is not mediated by deletional mechanisms, but by peripheral regulatory mech

145 fic tolerance through peripheral and central deletional mechanisms.

146 cell repertoire in chimeras indicates T-cell deletional mechanisms.

147 Furthermore, CD154 deletional mice irradiated with 200 cGy irradiation were

148 deletional mice, while IL-4 was absent from deletional mice, but up-regulated in controls.

149 s severity was found in MOG(35-55)-fed MCP-1 deletional mice, indicating induction of oral tolerance.

150 F-beta was up-regulated in fed and immunized deletional mice, while IL-4 was absent from deletional m

151 Deletional mutagenesis also indicate a biological role f

152 variant, we constructed C2delta(17) cDNA by deletional mutagenesis and expressed it transiently in C

153 Deletional mutagenesis localized these residues to a str

154 Deletional mutagenesis maps the RNA sequence causing agg

155 Deletional mutagenesis of individual SRE-like sequences

156 Deletional mutagenesis of the cytoplasmic domain of CD12

157 Deletional mutagenesis shows that the C-terminal 78 amin

158 PhoA fusions, deletional mutagenesis, and N-terminal amino acid sequen

159 By deletional mutagenesis, we found that deleting up to fou

160 Second, CTLA-4 deletional mutant (-/-) bone marrow chimeras were used a

161 L-60/Bcl-xLdelta cells that express the loop-deletional mutant cDNA constructs p19Bcl-2delta32-80 and

162 train of S. cerevisiae, human Prk, but not a deletional mutant protein, fully rescues the temperature

163 stably expressed the c-Jun dominant-negative deletional mutant TAM67, which lacks the NH(2)-terminal

164 amino acid residues in these two regions and deletional mutants missing residues 84-86 and 84-90 impl

165 Deletional mutants of HSPDE4B2B, which have been shown t

166 inducible cis-acting DNA elements, nested 5' deletional mutants of the icIL-1Ra promoter were constru

167 erminants of these differences, chimeric and deletional mutants of the N-terminal domain and the F re

168 Analysis of 7-19 amino acid deletional mutants that spanned the "regulatory module"

169 sicles of A549 cells while all COOH-terminal deletional mutants were restricted to an endoplasmic ret

170 Deletional mutation analysis confirmed that this locus i

171 chain using site-directed mutagenesis and/or deletional mutation.

172 Deletional mutations of HSL localized the region of HSL

173 Deletional mutations show that HSL interacts with the N-

174 crohomology and can join ends precisely; and deletional NHEJ (D-NHEJ), which utilizes microhomology t

175 nogenicity, focal expression of uUtro in the deletional-null German shorthaired pointer model produce

176 Transgenic Xenopus laevis were created with deletional or mutated promoter fragments driving an enha

177 The editing can be insertional, deletional, or substitutional and has been found in all

178 t CSR is programmed to occur in a productive deletional orientation and does so via an unprecedented

179 Productive CSR must occur in a deletional orientation by joining the upstream end of an

180 izes only D-RSSs or V(H)-RSSs in convergent (deletional) orientation with J(H)-RSSs(4-7).

181 the editing complex than the corresponding U deletional portion.

182 bination (CSR) occurs by an intrachromosomal deletional process between switch (S) regions in B cells

183 ccurs by a B cell-specific, intrachromosomal deletional process between switch regions.

184 In addition, the actual deletional process in thymi of the double-Tg mice was vi

185 sting that an immunoregulatory rather than a deletional process underlies this operational tolerance

186 y, we present the first analytical model for deletional rearrangement and show that it is consistent

187 i-CD152 (anti-CTLA-4) mAb or the use of CD28 deletional recipients abrogated engraftment in anti-CD15

188 Class switching occurs by a deletional recombination between two switch regions, eac

189 eavy (H) chain class switch is mediated by a deletional recombination event between micro and gamma,

190 ive switch (S) regions, with CSR involving a deletional recombination event between the donor Smu reg

191 occurs by a unique type of intrachromosomal deletional recombination within special G-rich tandem re

192 CSR generally occurs by an intrachromosomal deletional recombination within switch (S) region sequen

193 ype switch, where RNA processing may precede deletional recombination.

194 ocytes, and that it induces antigen-specific deletional responses in CD4(+) and CD8(+) T cells.

195 her and from previously characterized (early deletional) responses of naive CD4(+) T cells to PIT.

196 ival of transferred T-cells despite powerful deletional signals that mediate Bim (BCL2L11)-dependent

197 target effects from antibody-based Treg cell deletional strategies or small molecule drugging, we inv

198 id structures, which target nucleases in for deletional switch recombination.

199 to clinical use for the induction of central deletional T-cell tolerance.

200 However, the relative frequency of deletional to inversional CSR junctions has not been mea

201 medullary epithelial cells (MECs) to mediate deletional tolerance and thereby prevent autoimmunity.

202 del for the induction of mixed chimerism and deletional tolerance and thus further decreases the toxi

203 arrier for establishing chimerism, implicate deletional tolerance as the mechanism for total body irr

204 response to soluble Ags generally results in deletional tolerance following transient, costimulation-

205 s, but the role of CD28 in peripheral T cell deletional tolerance has not been investigated.

206 tigens that are not prominently presented in deletional tolerance likewise retain a high relative imm

207 Vbeta analysis suggested a central deletional tolerance mechanism.

208 ells are much less influenced by central and deletional tolerance mechanisms, and instead display a p

209 dendritic cells in a pathway culminating in deletional tolerance of peripheral alloreactive CD8 cell

210 , escape of retrovirus-specific B cells from deletional tolerance offers the opportunity to induce pr

211 Non-deletional tolerance resulting in the generation of regu

212 cell engraftment, a robust state of central deletional tolerance to allogeneic or xenogeneic donors

213 derscore the importance of peripheral T cell deletional tolerance to AQP4, which may be relevant to u

214 onses to liver-expressed antigens range from deletional tolerance to full effector differentiation re

215 we investigate a role for the Fas pathway in deletional tolerance to high dose bone marrow.

216 omal cells (LNSC) are important mediators of deletional tolerance to peripheral tissue-restricted ant

217 Evidence for deletional tolerance was obtained in mixed chimeras prep

218 by 3-Gy WBI allows the induction of central deletional tolerance while minimizing the toxicity of th

219 tant role for the thymus not only in central deletional tolerance, but also in the induction of perip

220 chimerism such protocols can permit central deletional tolerance, but with a significant risk of gra

221 n and suggest that in the absence of central deletional tolerance, peripheral tolerance mechanisms ar

222 Despite this apparent lack of deletional tolerance, repertoire inventories uncovered t

223 typically not presented during induction of deletional tolerance, retain a high degree of relative i

224 Unlike deletional tolerance, Treg cell-mediated tolerance was b

225 patibility complex antigen to further assess deletional tolerance.

226 pheral tissue antigens and induce CD8 T-cell deletional tolerance.

227 veloped durable multilineage MC and apparent deletional tolerance.

228 a CD4 T-cell response, indicating a lack of deletional tolerance.

229 finity tilted our system in the direction of deletional tolerance.

230 lopment of mixed hematopoietic chimerism and deletional tolerance.

231 syngeneic BMC, results in part from durable deletional unresponsiveness of graft-specific alloreacti

232 Transient transfection of deletional VCAM-1 promoter constructs and electrophoreti