ライフサイエンスコーパス: denaturation

1 erature range of 50-55 degrees C (myosin rod denaturation).

2 st inhibitory effect on heat-induced protein denaturation.

3 increased upon frozen storage due to protein denaturation.

4 ctor, but not the ribosome, protects against denaturation.

5 ct the structure of the protein against heat denaturation.

6 imity of structural proteins causing protein denaturation.

7 proteins to minimize protein misfolding and denaturation.

8 teractions relevant in urea-assisted protein denaturation.

9 cording to Privalov's interpretation of cold denaturation.

10 erved that adsorbed proteins undergo greater denaturation.

11 ting of amylose-lipid complexes plus protein denaturation.

12 r or sliding clamp) obtained during chemical denaturation.

13 can lead, possibly irreversibly, to protein denaturation.

14 hat the fluorescence of GFP is quenched upon denaturation.

15 how markedly enhanced sensitivity to thermal denaturation.

16 ation treatment for 10min indicating protein denaturation.

17 from that of Post-F-XC, as shown by chemical denaturation.

18 l conditions (room temperature) without heat denaturation.

19 lower protein yield as well as less protein denaturation.

20 h these epitopes are revealed with guanidine denaturation.

21 n a sequence-specific manner without causing denaturation.

22 enature proteins, i.e., thermal and chemical denaturation.

23 ed by analyzing nanomechanically induced DNA denaturation.

24 t results concerning the effects of chemical denaturation.

25 n-endemic malaria sera was abolished by heat-denaturation.

26 ed for extensive oxidation-dependent protein denaturation.

27 common unfolding event that reflects global denaturation.

28 lpha-Gal IgE epitopes were resistant to heat denaturation.

29 age or stress-induced protein misfolding and denaturation.

30 ater and in influencing chain expansion upon denaturation.

31 et structures, all of which indicate protein denaturation.

32 inhibition activity, which was removed upon denaturation.

33 s of SEE in preventing enzyme aggregation or denaturation.

34 ility of both serum albumins against thermal denaturation.

35 s of samples down to 1.9 mN/m due to protein denaturation.

36 proteins, resulting from stress such as heat denaturation.

37 at is resistant to both pH- and heat-induced denaturation.

38 in proteins not accessible by other means of denaturation.

39 eat treatments that can lead to whey protein denaturation.

40 rovides an excellent model to investigate FA denaturation.

41 tion of alpha-amylase due to ethanol-induced denaturation.

42 rbing treatments such as antibodies and cold denaturation.

43 rRhi o 2 showed partial refolding after heat denaturation.

44 I human leukocyte antigens (HLAs) upon acid denaturation.

45 ould not be refolded after solubilization by denaturation.

46 protect the enzyme's structure from thermal denaturation.

47 ion, which can result in partial or complete denaturation.

48 m aggressive environments, thus delaying the denaturation.

49 east frataxin, a protein that undergoes cold denaturation above zero degrees, because the unfolded fo

50 ability in bulk solution, as well as thermal denaturation, aggregation state, and biological activity

51 After denaturation, all fusions were completely renatured in s

52 ular dichroism spectra in far UV and thermal denaturation analyses demonstrate that axial ligand chan

53 Thermal denaturation analysis and protease-based peptide mass ma

54 SDS denaturation and acid denaturation of pSRII both lead to

55 s binds to other proteins and prevents their denaturation and aggregation through its chaperone-like

56 The patterns of denaturation and aggregation varied with pH.

57 s, among which heavy chain myosin, underwent denaturation and aggregation, as shown by a decrease in

58 e heat stability, and decreased whey protein denaturation and aggregation.

59 PEF) treatments combined or not with heat on denaturation and allergenicity of Pru p 3, the major all

60 cedure included sodium dodecyl sulfate (SDS) denaturation and chemical reduction of serum proteins to

61 ed NIR FPs, miRFP670nano is highly stable to denaturation and degradation and can be used as an inter

62 zwitterionic gel coating protected BChE from denaturation and degradation under harsh conditions.

63 e sensitivity of these constructs to thermal denaturation and demonstrate for the first time the bene

64 ed electrochemistry to monitor its guanidine denaturation and determine its folding free energy.

65 on allows for natural expansion upon protein denaturation and dissociation.

66 gested by the Lumry-Eyring model for protein denaturation and enzyme inactivation.

67 proteolysis of live cells in the absence of denaturation and fixation, heparin-affinity chromatograp

68 In conclusion, protein denaturation and formation of aggregates can be controll

69 reduces disulfide bonds and can drive mucin denaturation and microbial access to the mucus layer.

70 formation using UV- and CD-monitored thermal denaturation and on nucleoside conformation using (1)H N

71 stals growth in NAM to reduce protein freeze-denaturation and oxidation similarly to the commercial c

72 ivo complete protein digestion requires acid denaturation and pepsin, trypsin, and/or chymotrypsin cl

73 H values, and they are resistant to chemical denaturation and proteolysis.

74 xtremely stable against thermal and chemical denaturation and proteolytic degradation.

75 ide mimics can be readily designed to resist denaturation and proteolytic degradation.

76 s trap protons and cause less severe protein denaturation and reduced thaw loss.

77 By digesting proteins directly without denaturation and reduction, the HOS information can be r

78 to the four nsLTPs differed before and after denaturation and reduction, thus suggesting additional c

79 Denaturation and refolding behavior of the allergen conf

80 xide surface, protecting them from potential denaturation and rendering them sufficiently flexible to

81 eas hidden interface regions are revealed by denaturation and sequenced by MS.

82 of the 726 nm species during gentle protein denaturation and simple density functional theory calcul

83 Denaturation and subsequent aggregation of beta-lactoglo

84 ve subcellular structures which are prone to denaturation and subsequently release curcumin.

85 extremely resistant to chemical and thermal denaturation and that resist proteolysis by common prote

86 s were found to protect DNs against low-salt denaturation and to increase nuclease resistance by up t

87 exceptionally stable to thermal and chemical denaturation, and 12 experimentally determined X-ray and

88 nates the need for enzymatic reactions, heat denaturation, and complicated instruments during the det

89 cts such as non-specific adsorption, protein denaturation, and steric hindrance.

90 ultant flours seems to be related to protein denaturation as shown by decrease in nitrogen solubility

91 degrade at room temperature over time due to denaturation, as proteins unfold with loss of secondary

92 exhibit their highest stability against heat denaturation at pH 3.75.

93 Contrary, denaturation at pH 6.8 and 8.0 led to formation of non-n

94 Denaturation at the air-water interface is completely av

95 DeltaC1/2%, we compared relative changes of denaturation between peptides.

96 ueous solution, pushing the solution thermal denaturation beyond the boiling point of water.

97 structures, MANT-GTPgammaS binding, thermal denaturation, biochemical assays and sequence homology a

98 p and retain carbon monoxide, which prevents denaturation both in vitro and in vivo and explains the

99 a novel conformational regime for DNA, where denaturation bubbles form at the tips of plectonemes, an

100 nprotein matrix components, and the thorough denaturation by both surfactant and precipitation enable

101 o display ultra-high thermostability, resist denaturation by chaotropes and organic solvents, and hav

102 Denaturation by contact with air may happen at any stage

103 abilization of the Skp trimer and similar to denaturation by the nonnatural detergent lauryldimethyla

104 The large effect of PEG 20 on cold denaturation can be explained by a change in water activ

105 sothermal titration calorimetry, and thermal denaturation CD spectroscopy, we show that both subdomai

106 ge 20-50%, a sharp increase in the degree of denaturation, compared to the native protein solution, w

107 While denaturation curves obtained after exposure of PrP(Sc) t

108 Two had upshifted thermal denaturation curves when a peptide ligand was present, i

109 ecule force spectroscopy or existing thermal denaturation data.

110 The midpoint of denaturation declined with the removal of repeats.

111 present study demonstrated that serum sample denaturation decreases the test specificity due to relea

112 trophotometric data for determining the heat denaturation degree of bovine serum albumin (BSA) was as

113 well as increased stability against chemical denaturation [DeltaCm (GndHCl) = 0.53 M], without alteri

114 riment also supports the picture of chemical denaturation described in our simulations, driven by wea

115 iciency, which is not alleviated by altering denaturation/digestion stringency and indicates why prot

116 ion times indicated a lower degree of muscle denaturation during ageing in the chitosan dry-ageing be

117 The proteins of the IPPC underwent denaturation during extraction and some of the starch su

118 Its denaturation, either in vivo or in vitro, involves autox

119 00 W/cm(2), 2078 J/mL) significantly reduced denaturation enthalpies, whereas no change in secondary

120 enough to detect changes in the protein heat denaturation even at short temperature-time intervals.

121 y of AR by FoldX and guanidine hydrochloride denaturation experiment, and found that genetic disease-

122 The reason for this shift is that chemical denaturation experiments are labour-intensive, sample-co

123 Use of dynamic light scattering and thermal denaturation experiments delineates the compaction of th

124 This was confirmed with pH and urea denaturation experiments in conjunction with electron mi

125 Consistently, urea denaturation experiments indicate the stability of the W

126 This interpretation is supported by urea denaturation experiments performed on both PrP variants

127 Thermal denaturation experiments revealed that hm(5)rC increases

128 Invader probes are characterized by thermal denaturation experiments, analysis of thermodynamic para

129 tion mechanism of 1-3 has been determined by denaturation experiments, which reveal that the formatio

130 opy in combination with thermal and chemical denaturation experiments.

131 s study investigated the effect of pH on the denaturation extent, the surface chemical composition, t

132 The first strategy, using off-chip heat denaturation followed by sequential on-chip incubation o

133 Thermal denaturation has irreversible destabilizing effects on b

134 iological origin and exposed to instability, denaturation, high cost, and inherently low binding capa

135 Isothermal chemical denaturation (ICD) can be applied to determine chemical

136 ng of 32 chains from 16 heterodimer designs, denaturation in 5 M guanidine hydrochloride and reanneal

137 cence spectra indicated sarcoplasmic protein denaturation in drip due to freezing-thawing.

138 The role of protein denaturation in formation of thaw loss is currently not

139 udy aims at providing new insight on protein denaturation in freezing-thawing.

140 is the primary cause of myofibrillar protein denaturation in frozen-thawed meat.

141 ew of the non-locality of sequence-dependent denaturation in highly bent and supercoiled DNA loops, e

142 s range, which correlated with the extent of denaturation in the adsorbed state.

143 e epitope was sensitive to reduction and SDS denaturation in the isolated ricin domain and the larger

144 amic stability of each domain by equilibrium denaturation in urea.

145 Protein denaturation increased the reaction rate and reduced the

146 proteins decreased in the treated-milk, and denaturation increased with the treatment intensity.

147 both prion types to guanidine hydrochloride denaturation indicated this occurs because NAPA preclude

148 biological fluids can be compromised through denaturation induced by physiological salt concentration

149 led that cellular entrapment limited protein denaturation induced by thermal treatments.

150 efficiency, which is shown to correspond to denaturation-induced looped conformations that remain st

151 Protein denaturation involves a change in the protein structure

152 This low degree of heat denaturation is probably caused by the aggregation of th

153 Although chemical-induced denaturation is the gold-standard method, it has been re

154 f newly synthesized protein or after protein denaturation, is a substrate for binding by many cellula

155 etermine how protein composition affects the denaturation kinetics of the whey proteins within IMFs.

156 for heat stability, rheological properties, denaturation level of beta-lactoglobulin (beta-LG) and a

157 tate has a higher susceptibility to pressure denaturation (lower p1/2 and larger DeltaVu); thus, urea

158 cy therefore challenges our understanding of denaturation mechanism and more generally the accuracy o

159 create a high-throughput label-free chemical denaturation method that is capable of generating replic

160 -controlling effect of CCH on protein freeze-denaturation, NAM samples were subjected to 7 freeze-tha

161 ynamic hypothesis and the process of thermal denaturation, normally thought of as a cooperative two-s

162 By using circular dichroism, thermal denaturation, nuclear magnetic resonance spectroscopy, a

163 rophobicity, suggesting myofibrillar protein denaturation occurred by a comparable mechanism as in fr

164 ulfate (SDS) in situ bacteria lysing and DNA denaturation occurred in the high-temperature zone where

165 al antibodies and to detection of partial pH denaturation of a monoclonal antibody illustrates the pr

166 been shown to physically prevent the thermal denaturation of a number of model proteins.

167 as found to exhibit a maximum degree of heat denaturation of about 17% compared to the complete unfol

168 rmally activated processes contribute to the denaturation of adsorbed proteins.

169 Results showed that the heat-denaturation of alpha-lactalbumin was reduced when beta-

170 The kinetics of heat-induced denaturation of alpha-lactalbumin, beta-lactoglobulin an

171 present fully atomistic simulations of cold denaturation of an alpha-helical protein, the widely stu

172 tical model was developed to explain how the denaturation of an individual, adsorbed protein molecule

173 The reversible thermal denaturation of apo alpha-lactalbumin (alpha-LA) and lys

174 HHT at pH 6.6 resulted in denaturation of approximately 67% of total whey protein,

175 cooking with wood ashes and Ca(OH)2 produced denaturation of bean proteins, decreasing the second tra

176 Hence, in this study total denaturation of beta-lactoglobulin was performed at defi

177 s of moderate electric fields during thermal denaturation of beta-lactoglobulin were examined through

178 the IMFs but its presence enhanced the heat-denaturation of beta-lactoglobulin.

179 hydryl-disulfide interchange reaction during denaturation of beta-LG and alpha-LA.

180 tor the temperature-dependent adsorption and denaturation of bovine serum albumin (BSA) protein onto

181 Complete denaturation of BSA occurred only when the gelation temp

182 The denaturation of collagen causes predictable structural c

183 TSSA-I glycosylation but retained after heat-denaturation of conformation.

184 all fraction of polypeptides could lower the denaturation of crayfish myosin heavy chain when compare

185 tegy that involves (i) initial isolation and denaturation of global DNA using the standard isolation

186 lded (denatured) state, we study the thermal denaturation of hydrated lysozyme that occurs when the t

187 d consequences on the catalytic activity and denaturation of infiltrated enzymes.

188 The heat-denaturation of lactoferrin was not affected by the comp

189 We achieved complete denaturation of mAbs using a straightforward protocol, a

190 Notably, the denaturation of MBD1 disassembled the DMA and accordingl

191 This study investigated denaturation of myofibrillar and sarcoplasmic proteins o

192 e hydrophobicity, indicating more pronounced denaturation of myofibrillar proteins.

193 Ionic-liquid-induced denaturation of one of the enzymes is indicated over the

194 sed in the process of crystallization causes denaturation of pathological tissue proteins (metastasis

195 eratures < 60 degrees C, the greater thermal denaturation of proteins in cutaneous trunci than in mas

196 inization of starch in the flour and partial denaturation of proteins.

197 Denaturation of Pru p 3, determined by ELISA using rabbi

198 SDS denaturation and acid denaturation of pSRII both lead to fraying of helix ends

199 port to describe a temperature threshold for denaturation of rutin-degrading enzymes in any buckwheat

200 However, in vivo degradation or denaturation of surface constituents often limits the lo

201 e complete starch gelatinization and protein denaturation of the bean powders and thus changed their

202 measurement cycles is attributed to partial denaturation of the enzyme.

203 ated brine, increasing the risk of microbial denaturation of the fillets during storage.

204 1 is demonstrated by monitoring the thermal denaturation of the following double and triple helical

205 haracterized by the states of ionization and denaturation of the immobilized urease, as such the mode

206 g test without the need for purification and denaturation of the PCR products.

207 and fluorescence spectroscopy confirmed that denaturation of the proteins during the acid precipitati

208 and 95 degrees C, rutin was retained due to denaturation of the rutin-degrading enzymes during hydro

209 Interestingly, there is no concomitant denaturation of the secondary structural elements of Rsn

210 control at temperatures 60 degrees C caused denaturation of the whey proteins and aggregation of the

211 heat, pH, ultrasonication and ethanol on the denaturation of whey protein isolate.

212 The enthalpy change (DeltaH of denaturation) of whey proteins decreased in the treated-

213 tationary phase interactions, and/or protein denaturation, often is not assessed.

214 dvantage of high-speed DNA hybridization and denaturation on beads under flow conditions, high fideli

215 d conformations showing better resilience to denaturation on cross-linked species using ion mobility.

216 ion of the therapeutic CD38 antibody or CD38 denaturation on reagent red blood cells mitigates daratu

217 the effect of temperature-dependent protein denaturation on the corresponding adsorption kinetics.

218 in and the influence of pH value and protein denaturation on this reaction have been examined in the

219 ion technique without the need of additional denaturation or cross-linking steps for stabilization.

220 , NFs are resistant to nuclease degradation, denaturation or dissociation at extremely low concentrat

221 Food processing causes decomposition, denaturation or polymerization of protein, which may alt

222 sus temperature that is not accounted for by denaturation or unfolding.

223 an interface is an arrested state along the denaturation pathway.

224 We determined this via guanidinium denaturations performed in the presence of varying conce

225 e findings suggest that thermal and chemical denaturation perturb the structure of prion protein aggr

226 her derive from our observations a schematic denaturation phase diagram together with energy landscap

227 In contrast to sequential chemical denaturation, phycocyanin's chromophore and protein stru

228 e which, in conjunction with a short thermal denaturation, prevents renaturation of the duplex nuclei

229 The observed enhancement in the cold denaturation process upon ice formation can be mitigated

230 the unfolding and aggregation phases of the denaturation process, whereas such a discrimination coul

231 d higher resistance to thermal and reductive denaturation processes, and the presence of C-mannoses p

232 gnificant strain-specific differences in the denaturation profile of the protein, treatment with guan

233 The individual fit of the pressure denaturation profiles gives rise to first order, GNMR, a

234 , even though they exhibit identical thermal denaturation profiles.

235 h rasburicase (known to be very sensitive to denaturation), proving the structural stability of the p

236 nvolve bacterial culture, protein isolation, denaturation, purification and finally protein assembly.

237 Based on a model of protein denaturation rate limited by an entropy-related barrier,

238 ing at this pH value, the supplementary heat denaturation rate was lower compared to that at neutral

239 The highest heat denaturation rates were observed at pH values higher tha

240 iched simultaneously, followed by a one-step denaturation, reduction, and alkylation.

241 likely because surfactant permits extensive denaturation/reduction/alkylation of proteins and inacti

242 was confirmed using limited proteolysis and denaturation/refolding.

243 wild-type control samples, subjected to one denaturation/renaturation cycle and products are electro

244 -PCR) strategy by exploiting ion-induced DNA denaturation/renaturation cycles.

245 to thermal cycling, 30 cycles of pH-induced denaturation/renaturation were used to amplify protein D

246 imitations in quantitative protein transfer, denaturation, solubility, or stability.

247 ea that accounts for both the ionization and denaturation states of the urease subject to the environ

248 ons spanning 13 years implicate the alkaline denaturation step as a significant cause for inefficient

249 -splicing at ambient temperatures, without a denaturation step, and in the absence of reducing agents

250 Thermal denaturation studies and footprinting experiments confir

251 Thermal denaturation studies demonstrate that, under reducing co

252 The use of thermal denaturation studies in conjunction with UV spectrophoto

253 Thermal denaturation studies revealed that the hydrophobic appen

254 Maintaining the protein structure without denaturation/substantial conformational change and contr

255 esistant to proteolytic cleavage and thermal denaturation, suggesting that the association of regulat

256 f fat melting (Tmelting) or xanthine oxidase denaturation (Tdenaturation), whereas thermal treatments

257 influence of amino acid additives on thermal denaturation temperature (T(d)) and heat-set gelation of

258 ed SCRII was much more thermostable with its denaturation temperature (Tm) increased to 60 degrees C.

259 ed over a temperature range that spanned the denaturation temperature (Tm) of hybrids by applying a s

260 lly active E. coli DHFR mutant with measured denaturation temperature 7.2 degrees C higher than WT.

261 The denaturation temperature of FPI was 105 degrees C.

262 ing alone decreased the enthalpy and thermal denaturation temperature of the connective tissue protei

263 The connective tissue denaturation temperature was lower for the FP group, coi

264 energy of the native state and experimental denaturation temperature was much weaker, indicating the

265 was not enough for significant alteration of denaturation temperature.

266 alpha1-PI showed high thermal stability with denaturation temperatures of 65.43 and 73.31 degrees C,

267 The reduced thermal denaturation temperatures of the T61A, S93A, and H266A F

268 Higher denaturation temperatures were observed in treated prote

269 cular dichroism spectroscopy to measure half-denaturation temperatures, our results show that protein

270 CPI was more susceptible to thermal denaturation than GCPI as determined by turbidity and su

271 type II fibres is more sensitive to thermal denaturation than myosin in type I fibres and this shoul

272 leavedform possesses more resistance to such denaturation than the intactform.

273 bean protein (TMBP) with favourable partial denaturation, the formation of small aggregates, improve

274 By thermal denaturation, the MOSP(N) and MOSP(C)-like domains of Tp

275 oisture content resulted in complete protein denaturation, the undesirable formation of large aggrega

276 nding but changed the susceptibility to heat denaturation: the thermodynamic stability of A(1)R-G279S

277 with very high firmness without whey protein denaturation; the firmness was similar to gels from heat

278 able with the human eye as a function of the denaturation time.

279 1:2 complexes were determined by UV-vis-NIR denaturation titration, using pyridine as a competing li

280 % feed moisture prompting favourable partial denaturation to produce TMBP with desirable qualities fo

281 in combination with scattering upon thermal denaturation to study the unfolding of integral membrane

282 eriments (ultraviolet absorption for thermal denaturation) to analyze the differential stability of d

283 mounts of isobaric label (levels that induce denaturation) to enhance the sensitivity and resolution

284 ise, not only in protecting the enzymes from denaturation under nonbiological environments, but also,

285 We first calibrated FA denaturation using three small, globular, and acid-resis

286 odifications, reflected in decreased thermal denaturation values (DeltaTm, ca. 2.5-11.5 degrees C).

287 Xanthine oxidase denaturation was approximately 13% less after PEF treatm

288 However, sarcoplasmic protein denaturation was independent of freezing rate.

289 lts seem to follow the Hofmeister series: no denaturation was induced using ammonium acetate (kosmotr

290 ary model based on the mechanisms of protein denaturation was then added to take into account the eff

291 More severe muscle fibre coagulation and denaturation were observed in the shockwave-treated cook

292 extremely stable toward thermal and chemical denaturation: whereas TriEst was unfolded by heating ( T

293 ction takes place without detectable protein denaturation, which might be revealed by heme loss or an

294 es in the treated samples related to protein denaturation, which was probably responsible for the dec

295 , i.e. 2.5, they exhibited considerable cold denaturation, while after heating at this pH value, the

296 thod for estimating the debated end point of denaturation, while already the 1D (1)H spectrum gives a

297 of duplex formation using ITC and UV thermal denaturation with RNA duplexes containing internal s(2)U

298 This method combines protein denaturation with the diffusion filter to produce clean

299 ques to study the sequence-dependence of DNA denaturation within DNA minicircles, we have observed th

300 from DNA brick motifs remain stable against denaturation without any chemical modifications.