ライフサイエンスコーパス: dense granule

1 on of only one major platelet organelle, the dense granule.

2 between proteins secreted from rhoptries and dense granules.

3 f the murine ashen (Rab27a) gene in platelet-dense granules.

4 utive secretory vesicles of tachyzoites, the dense granules.

5 cifically regulates the contents of platelet-dense granules.

6 mal system, such as melanosomes and platelet dense granules.

7 organelles, such as melanosomes and platelet dense granules.

8 organelles such as melanosomes and platelet-dense granules.

9 ined to melanosomes, lysosomes, and platelet dense granules.

10 organelles such as melanosomes and platelet dense granules.

11 organelles such as melanosomes and platelet-dense granules.

12 alized to the Golgi of T. gondii, but not to dense granules.

13 t of the role of TgARF1 in release of intact dense granules.

14 esis of melanosomes, lysosomes, and platelet dense granules.

15 es and a reduction in the number of platelet dense granules.

16 tion of melanosomes, lysosomes, and platelet dense granules.

17 , beta-lactamase, that localizes to parasite dense granules.

18 C) transporter, ABCC4, functions in platelet-dense granules.

19 nous dense granule protein GRA4 localized to dense granules.

20 Toxoplasma gondii are derived from parasite dense granules.

21 rofilaments, some mitochondria, vacuoles and dense granules.

22 man HPS patients in melanosomes and platelet-dense granules.

23 drives host cell remodelling and occurs from dense granules.

24 ncy, reflecting the malformation of platelet dense granules.

25 ultrastructurally as intracellular electron-dense granules.

26 hates are enriched in insoluble and electron-dense granules.

27 lted in a marked dose-dependent secretion of dense granules.

28 BCC4), which facilitates ADP accumulation in dense granules.

29 ared from Day 3 highlighting the presence of dense granules.

30 tein-3 are necessary for normal transport to dense granules.

31 ansky-Pudlak syndrome, ruby-eye, which lacks dense granules.

32 eted effectors rhoptry 16 kinase (ROP16) and dense granule 15 (GRA15) activate the JAK-STAT3/6 and NF

33 Lysosomal and platelet dense granule abnormalities, including hyposecretion of

34 Contents released from dense granules after platelet activation promote coagula

35 Dense granules also are targeted by antiplatelet agents

36 Dense granule and alpha-granule release are reduced in t

37 ral role in amplifying platelet aggregation, dense granule and alpha-granule secretion, P-selectin ex

38 ated transport vesicles, and microdomains of dense granule and endosomal membranes.

39 herefore, the molecular understanding of the dense granule and its biogenesis is of vital importance.

40 were delivered quantitatively into parasite dense granules and efficiently secreted into the vacuola

41 secrete contents of both alpha-granules and dense granules and generate thromboxane A2 (TXA2), but p

42 inorganic phosphate, is present in platelet dense granules and is secreted on platelet activation.

43 lving abnormalities of melanosomes, platelet dense granules and lysosomes.

44 ian organelles such as melanosomes, platelet dense granules and lysosomes.

45 result from defects of melanosomes, platelet dense granules and lysosomes.

46 ytoplasmic organelles: melanosomes, platelet dense granules and lysosomes.

47 organelles, including melanosomes, platelet dense granules and lysosomes.

48 C-2) functions in the biogenesis of platelet dense granules and melanosomes, which like WPBs are lyso

49 cretions of another class of organelles, the dense granules and osmiophilic bodies, are proposed to b

50 The synthesis of dense granules and other lysosome-related organelles is

51 The mass-dense granules and the contractile vacuole appeared to c

52 primarily to the Golgi, although staining of dense granules and the intravacuolar network was also de

53 Targeting of secreted proteins to T. gondii dense granules and the plasma membrane uses general mech

54 lized organelles (rhoptries, micronemes, and dense granules) and the capture of host materials.

55 ggregometry, 16 of 35 had decreased platelet dense granules, and 28 of 55 had abnormal bleeding score

56 s was reduced secretion from alpha-granules, dense granules, and lysosomes following CRP stimulation.

57 cytoplasmic organelles-melanosomes, platelet-dense granules, and lysosomes.

58 cytoplasmic organelles-melanosomes, platelet-dense granules, and lysosomes.

59 ng the apicoplast (ClpB1), rhoptries (RON6), dense granules, and parasitophorous vacuole (EXP2, PTEX1

60 sma apical secretory organelles (micronemes, dense granules, and rhoptries) play key roles in host ce

61 s), involved in the genesis of rhoptries and dense granules, and TgBAR2 found at the parasite cortex.

62 te organelles, namely micronemes, rhoptries, dense granules, and the apicoplast.

63 appearance, contained abundant intracellular dense granules, and were surrounded by a less-dense matr

64 iation to mature Mks synthesizing alpha- and dense-granules, and forming PPTs without exogenous throm

65 mature protease is concentrated in merozoite dense granules, apical secretory organelles involved in

66 s, CgA-expressing A35C cells showed electron-dense granules approximately 180-220 nm in diameter, and

67 Lysosomes, melanosomes and platelet-dense granules are abnormal in the mouse hypopigmentatio

68 Hermansky-Pudlak syndrome in which platelet dense granules are absent.

69 Dense granules are important in platelet aggregation to

70 der the cytoplasmic membrane, a region where dense granules are known to migrate after maturation.

71 Platelet dense granules are lysosome-related organelles which con

72 Platelet dense granules are members of a family of tissue-specifi

73 Toxoplasma gondii dense granules are morphologically similar to dense matr

74 Thus, essentially normal numbers of platelet dense granules are produced but the granule interiors ar

75 rthermore, the presence of numerous electron-dense granules around the phagosomes indicated that neut

76 ne nucleotides though near-normal numbers of dense granules as enumerated by the dense granule-specif

77 organelles such as melanosomes and platelet dense granules as well as to neurotransmitter vesicles.

78 MRP4 redistributed cAMP from the cytosol to dense granules, as confirmed by increased vasodilator-st

79 This work sheds light on the biogenesis of dense granules at the molecular level and opens the poss

80 ranules, MHC class II compartments, platelet-dense granules, basophil granules, azurophil granules, a

81 arly endosomes as MKs mature or functions in dense granule biogenesis directly from early endosomes,

82 rome is now known to be related to defective dense granule biogenesis due to mutations in any of >/=9

83 (MEG-01) as a model system for the study of dense granule biogenesis using a variety of cell biology

84 w that early endosomes play a direct role in dense granule biogenesis.

85 sed Golgi staining and increased delivery to dense granules but blocked delivery to the intravacuolar

86 MP-3, whereas rVAMP-8 inhibited secretion of dense granules but not alpha granules.

87 dom is the presence of perinuclear, electron-dense granules called nuage.

88 rucial for the fusion of vesicles containing dense granule cargo with the maturing organelle.

89 Ap3A is a platelet-dense granule component released into the extracellular

90 n background have greatly reduced amounts of dense granule components such as serotonin and adenine n

91 he effects of the ashen mutation on platelet-dense granule components, platelet aggregation, and blee

92 g that sporozoites have a different electron-dense-granule composition, we have now found that sporoz

93 g that the impairment is secondary to absent dense granule content release.

94 ha-granule proteins and reduced secretion of dense granule contents critical for platelet activation.

95 e secretagogue which promotes the release of dense granule contents; (2) colocalization with ACTH, an

96 rate that platelets release their alpha- and dense-granule contents in both non-severe and severe for

97 we used SLC35D3, mutation of which causes a dense granule defect in mice, to show that early endosom

98 The platelet-dense granule defect is rescued in BAC transgenic mice c

99 tion promote coagulation and hemostasis, and dense granule defects such as those seen in Hermansky-Pu

100 reased bleeding time in mice and humans with dense granule deficiency.

101 ) who are specifically deficient in platelet dense granules (delta-SPD) have suggested a role for den

102 s also observed in mice that lacked platelet-dense granules, dense granule secretion machinery, glyco

103 Centriolar satellites are numerous electron-dense granules dispersed around the centrosome.

104 ing diathesis due to the absence of platelet dense granules, displays extensive locus heterogeneity.

105 equired for efficient secretion of alpha and dense granules downstream of G(13) and G(q).

106 d MAF1 encodes distinct paralogs of secreted dense granule effector proteins, some of which mediate t

107 ) platelets demonstrated a partial defect in dense granule exocytosis and impaired aggregation.

108 unc18c with syntaxin enhanced Ca2+-triggered dense granule exocytosis in permeabilized cells.

109 The site(s) of dense granule exocytosis, however, has been unknown.

110 e to release of agonists other than ADP from dense granules, experiments were performed on murine pla

111 sive bleeding, but little is known about how dense granules form in megakaryocytes (MKs).

112 that SLC35D3 is either delivered to nascent dense granules from contiguous early endosomes as MKs ma

113 The composition of these crystals and the dense granules from which they are derived has remained

114 Platelets lacking ABCC4 have unchanged dense-granule function, number, and volume, but harbor a

115 Serotonin, an abundant component of platelet-dense granules, has an Mr of 176, and fibrinogen isolate

116 l portion of the protein constituents of the dense granules have been identified, and little is known

117 side the parasite that are distinct from the dense granules; however, in the encysted bradyzoite stag

118 Pactolus protein is held within the cell in dense granules in a highly glycosylated form.

119 tron microscopy revealed a reduced number of dense granules in affected patients platelets, correlati

120 tivity localized to the cytoplasmic electron-dense granules in ASPS.

121 nalysis revealed a higher number of electron-dense granules in Ctr2(-/-) mast cells than in wild-type

122 strated a combination of ASPS-like features (dense granules in four cases, rhomboid crystals in two c

123 Rab27b localizes to alpha and dense granules in megakaryocytes.

124 ckout mice showed increased numbers of small dense granules in the granular layer with few or no surr

125 Platelet aggregation and secretion from dense granules induced by CRP and thrombin was slightly

126 TgPI-1(41) are secreted constitutively from dense granules into the excreted/secreted antigen fracti

127 PI1 isoforms, both of which are secreted via dense granules into the parasitophorous vacuole shortly

128 The dense granule localization of the single pass transmembr

129 a length similar to that of GRA4 resulted in dense granule localization, whereas lengthening the GRA4

130 w demonstrate that NO inhibits exocytosis of dense granules, lysosomal granules, and alpha-granules f

131 the related subcellular organelles platelet dense granules, lysosomes, and melanosomes.

132 We compared the secretion of the endogenous dense granule marker GRA3 in Toxoplasma gondii with the

133 platelet membrane, and provide evidence that dense granules may be a major source of ADP which can co

134 uggest a close relationship between platelet dense granules, melanosomes of melanocytes and secretory

135 ndent fashion, inducing release of alpha and dense granules, membrane alterations, aggregation, and f

136 elet releasates (the 'secretome'), alpha and dense granules, membrane and cytoskeletal proteins, plat

137 This is consists of dense granules, mitochondria, and specific localised RNA

138 of orthophosphate moieties released from the dense granules of activated platelets, is a procoagulant

139 ogether, these results suggest that the mass-dense granules of D. discoideum are homologous to the ac

140 The mass-dense granules of Dictyostelium discoideum were shown to

141 invasion these components reside within the dense granules of invasive merozoites.

142 finity method detected histamine in electron-dense granules of mast cells in control and injected ski

143 of plants (tonoplast) and the small electron-dense granules of some parasites (acidocalcisomes) where

144 d to the surface of the apical region and to dense granules of sporozoites and merozoites.

145 eriments revealed that TgPSD1 resides in the dense granules of T. gondii and is also found in the par

146 n the biogenesis of melanosomes and platelet dense granules, often referred to as lysosome-related or

147 from exposure to cell extracts that contain dense granule or micronemal proteins.

148 ator of protein transport between post-Golgi dense granule organelles and the Golgi.

149 BD-12 was localized exclusively to the novel dense granules, organelles that also contain precursors

150 rectly from early endosomes, suggesting that dense granules originate from early endosomes in MKs.

151 Platelet dense granules (PDGs) are acidic calcium stores essentia

152 r, Escherichia coli alkaline phosphatase, to dense granules, precluding an in vivo assessment of the

153 predominantly to early endosomes but not to dense granule precursors.

154 site strains and mouse species, of which the dense granule protein 12 (GRA12) emerged as the most imp

155 Dense granule protein 12 (GRA12) is implicated in a rang

156 mosome X that included the gene encoding the dense granule protein 15 (GRA15).

157 cognize the N-terminal region (aa 41-152) of dense granule protein 6 (GRA6Nt) of the parasite present

158 inases rhoptry proteins 8 and 2 (ROP8/2) and dense granule protein 7 (GRA7).

159 These results show for the first time that a dense granule protein can modulate host signaling pathwa

160 H-2L(d)-restricted T cell epitopes, one from dense granule protein GRA4 and the other from rhoptry pr

161 ain and cytoplasmic tail from the endogenous dense granule protein GRA4 localized to dense granules.

162 We show that one of these genes encodes dense granule protein GRA45, which has a chaperone-like

163 Here we show that the dense granule protein GRA7 is phosphorylated but only in

164 ersion of TgPL1 partially colocalized with a dense granule protein in the parasitophorous vacuole spa

165 Within the vacuole, the 28-kDa dense granule protein known as GRA2 is specifically targ

166 tion status of the NTPase, the only parasite dense granule protein that contains disulfide bonds, is

167 lt of the polymorphic protein GRA15, a novel dense granule protein which T. gondii secretes into the

168 port that Toxoplasma secretes GRA24, a novel dense granule protein which traffics from the vacuole to

169 In this report, we identify a novel secreted dense granule protein, GRA14, and show that it is target

170 rophage gene expression, we identify a novel dense granule protein, GRA25.

171 dominant CD8 T cell response to the parasite dense-granule protein GRA6 cannot be generated, leads to

172 y after merozoite invasion and at least some dense granule proteins also use the alternate pathway.

173 in can modulate host signaling pathways, and dense granule proteins can therefore join rhoptry protei

174 MIC2, the cyst matrix protein MAG1, and the dense granule proteins GRA4 and GRA7, were commonly reco

175 We previously demonstrated that secretion of dense granule proteins in permeabilized parasites was au

176 a GDP-bound (Rab6(T25N)) mutant accumulated dense granule proteins in the Golgi and associated trans

177 ic approaches, GTPgammaS enhanced release of dense granule proteins in the permeabilized cell system.

178 rmore, this integrative analysis divides the dense granule proteins into heterogeneous populations su

179 By mistargeting of mutant dense granule proteins, we demonstrate that sorting sign

180 extran with both mepacrine and transmembrane dense granule proteins.

181 , however, sporozoites did not exocytose the dense-granule proteins GRA1, GRA2, or GRA4 during PV1 fo

182 Other tachyzoite dense-granule proteins, GRA1, GRA2, GRA4, GRA5, and GRA6

183 An analysis of human platelet dense granules, purified using metrizamide gradient cent

184 SFLLRN- or AYPGKF-induced dense granule release and PKCdelta phosphorylation occur

185 imary and secondary waves of aggregation and dense granule release are strongly induced by nanomolar

186 mechanism of such differential regulation of dense granule release by PKC-delta in platelets.

187 rresponsive CRP and CLEC-2-induced alpha and dense granule release compared with wild-type platelets.

188 These results demonstrate the importance of dense granule release even in the earliest phases of thr

189 a time-dependent manner that coincided with dense granule release in response to protease-activated

190 ombin, collagen, and thromboxane A(2), cause dense granule release independently of thromboxane gener

191 soforms play a differential role in platelet dense granule release mediated by protease-activated rec

192 NSF/SNAP/SNARE/Rab machinery participates in dense granule release using parasite protein components

193 Furthermore, AYPGKF and SFLLRN-induced dense granule release was blocked by rottlerin, a PKCdel

194 However, SFLLRN-induced dense granule release was unaffected in the presence of

195 platelet CD62P expression, alpha-granule and dense granule release, and the classical morphological c

196 albicans does not appear to affect alpha or dense granule release, C. albicans exerts a significant

197 hyperresponsiveness to ADP is independent of dense granule release, cyclooxygenase-derived eicosanoid

198 role of individual PKC isoforms in platelet dense granule release.

199 e invasion (named rhoptries, micronemes, and dense granules), remains poorly understood, particularly

200 Specifically, we show that dense granules require these structures for the secretio

201 antibodies against proteins from micronemes, dense granules, rhoptries, and plasma membrane showed th

202 Only agonists that caused platelet dense granule secretion activated PKCdelta.

203 hibited the P2Y(12)-mediated potentiation of dense granule secretion and Akt phosphorylation, and did

204 n of 12-LOX or PKC resulted in inhibition of dense granule secretion and attenuation of both aggregat

205 Aggregation, dense granule secretion and calcium mobilisation were si

206 association with a significant reduction in dense granule secretion and impaired aggregation to a pa

207 STX8 therefore specifically contributes to dense granule secretion and represents another member of

208 UNX1 may be common in patients with platelet dense granule secretion defects and mild thrombocytopeni

209 understanding of the disease and the role of dense granule secretion in platelet function.

210 sociations negatively regulate GPVI-mediated dense granule secretion in platelets.

211 haIIbbeta3 activation, and alpha-granule and dense granule secretion in response to the glycoprotein

212 /-) platelets showed a significant defect in dense granule secretion in response to thrombin and CRP.

213 Defective dense granule secretion in RhoG(-/-) platelets limited r

214 on G3 column and able to bind GTP stimulated dense granule secretion in the permeabilized cell secret

215 embryos in which platelet alpha-granule and dense granule secretion is abolished.

216 in mice that lacked platelet-dense granules, dense granule secretion machinery, glycoprotein (GP) VI,

217 In contrast, convulxin-induced dense granule secretion was potentiated by rottlerin but

218 In comparison, both aggregation and dense granule secretion were normal following activation

219 ated enhanced agonist-dependent aggregation, dense granule secretion, and fibrinogen binding, compare

220 ith excessive bleeding and impaired platelet dense granule secretion, and highlight transcription fac

221 on triggering adhesion, aggregation, massive dense granule secretion, and thromboxane production.

222 functional responses, including aggregation, dense granule secretion, and TXA(2) generation, compared

223 role in protease-activated receptor-mediated dense granule secretion, whereas it functions as a negat

224 PKC-delta positively regulates PAR-mediated dense granule secretion, whereas it negatively regulates

225 For instance, PKCd is known to regulate dense granule secretion, which is important for platelet

226 hereas it negatively regulates GPVI-mediated dense granule secretion.

227 duced alpha-granule secretion but stimulated dense granule secretion.

228 d differentially regulates alpha-granule and dense granule secretion.

229 cretion with comparatively modest effects on dense granule secretion.

230 ted peptide, and GPVI/FcRgamma-chain-induced dense granule secretion.

231 aggregation, and severely reduced alpha and dense granule secretion.

232 ng that they are a consequence of diminished dense granule secretion.

233 receptor signaling, the TxA(2) pathway, and dense granule secretion.

234 egree of lysosomes, is secondary to impaired dense granule secretion; and (3) diminished alpha granul

235 measurement of P-selectin) was blocked, and dense-granule secretion (assessed by release of carbon 1

236 activation-dependent increases of alpha and dense-granule secretion and integrin alphaIIbbeta3 activ

237 ence motifs inhibited both alpha-granule and dense-granule secretion in permeabilized human platelets

238 To examine the role of platelet dense-granule secretion in these processes, atherosclero

239 In atherosclerotic mice, reduced platelet dense-granule secretion is associated with marked protec

240 re, we examined the hypothesis that platelet dense-granule secretion modulates thrombosis, inflammati

241 production, adenosine diphosphate (ADP) and dense-granule secretion, and alpha(IIb)beta(3)-mediated

242 l2(-/-) mice, which lack platelet alpha- and dense-granule secretion, show no signs of hemorrhage in

243 trate that cancer cells can promote platelet dense-granule secretion, which is required to augment pl

244 3 gene (HPS3(-/-)) markedly reduces platelet dense-granule secretion.

245 etions are potentiated, whereas PAR-mediated dense granule secretions are inhibited.

246 atelets lacking Lyn or SHIP-1, GPVI-mediated dense granule secretions are potentiated, whereas PAR-me

247 TgLCAT is stored in a subpopulation of dense granule secretory organelles, and following secret

248 n rhoptry proteins, but not in proteins from dense granule secretory organelles; (c) when mutated in

249 or GTP-activated Rab6(Q70L) rerouted soluble dense granule secretory proteins to the Golgi and endopl

250 surrounding the parasite is remodeled by the dense granules, secretory organelles that release an arr

251 me ultrastructure, and in levels of platelet dense granule serotonin, the corresponding phenotypes of

252 t adenosine 5'-diphosphate (ADP) released by dense granules serves as an autocrine signal to potentia

253 ferent organelles--micronemes, rhoptries and dense granules--serves to establish and maintain a paras

254 mbers of dense granules as enumerated by the dense granule-specific fluorescent dye mepacrine.

255 We found no role of MRP4 in ADP dense-granule storage, but MRP4 redistributed cAMP from

256 Human platelet dense granules strongly resemble acidocalcisomes, and we

257 The role of dense granule substances in mediating platelet adhesion

258 anules (delta-SPD) have suggested a role for dense granule substances, in all likelihood adenosine di

259 nd associated with micronemes, rhoptries, or dense granules, the three identified secretory organelle

260 in mice diminishes polyphosphate in platelet dense granules, thereby reducing hemostasis and protecti

261 ogenous secretion systems, the rhoptries and dense granules, to deliver multiple large (>100 kDa) the

262 tein release from secretory vesicles, called dense granules, to maintain the parasite's intracellular

263 All of these characteristics of the platelet dense granules, together with their known acidity and hi

264 adenosine 5'-diphosphate (ADP) secreted from dense granules, trigger platelet activation.

265 through site-specific serotonin release from dense granules, triggering proliferative signaling in he

266 s confirmed by visualization of polyP in the dense granules using 4',6-diamidino-2-phenylindole and b

267 ll as release of adenosine triphosphate from dense granules was also defective in Akt-1-null platelet

268 Release from dense granules was completely ablated and that from alph

269 +)-pyrophosphatase activity of isolated mass-dense granules was stimulated by potassium ions and inhi

270 Dense granules were also shown to contain large amounts

271 tural alterations other than those involving dense granules were detected.

272 sion of serotonin concentrations of platelet dense granules were likewise more severe in double than

273 ive mature protease (p47) is concentrated in dense granules, which are secretory organelles located t

274 Rhoptries, micronemes, and dense granules, which are secretory organelles unique to

275 merous proteins delivered from rhoptries and dense granules, which are secretory organelles unique to

276 urons containing distinctive large, electron-dense granules, which could reliably be used to identify

277 ctron dense deposits at E15 to more electron dense granules with complex patterns of internal structu

278 , known as polyphosphate (polyP), which form dense granules within the cell.