ライフサイエンスコーパス: derivatization

1 thout any previous sample preparation (i.e., derivatization).

2 metry data set with postcolumn Paterno-Buchi derivatization.

3 cal activity, or intermediates for synthetic derivatization.

4 in high yields and selectivities for further derivatization.

5 he number of (13)C atoms incorporated during derivatization.

6 e compared with HPLC coupled with pre-column derivatization.

7 uires a combination of methods and/or sample derivatization.

8 unds an intriguing class of dyes for further derivatization.

9 ples), including examples of late-stage drug derivatization.

10 strated equivalent results with conventional derivatization.

11 acids were analysed after automated on-line derivatization.

12 ese critical intermediates without synthetic derivatization.

13 tive for many alkene classes and facilitated derivatization.

14 HP) in human urine is possible without prior derivatization.

15 -tandem mass spectrometry (LC-MS/MS) without derivatization.

16 highly reactive fluorescent tags for glycan derivatization.

17 of other lipids during liquid-phase analyte derivatization.

18 thyltrifluoroacetamide (MTBSTFA) for analyte derivatization.

19 coproteins and tissue samples after chemical derivatization.

20 alogy to the classic procedure of heavy-atom derivatization.

21 to distinguish PCs and PSs without chemical derivatization.

22 not have an easy "handle" for bioreversible derivatization.

23 ion in comparison with phenylhydrazine (PHN) derivatization.

24 nal handles and vectors suitable for further derivatization.

25 sis, and one of the products is utilized for derivatization.

26 enzyme deconjugation and precolumn chemical derivatization.

27 eed for time-consuming sample preparation or derivatization.

28 n further into the NIR region through select derivatizations.

29 the conformational modulation induced by the derivatizations.

30 uantum mechanical calculations, and chemical derivatizations.

31 for the glycodiversification through further derivatizations.

32 representative stereoretentive postcatalytic derivatizations.

33 On-tissue analyte derivatization addresses these issues by selectively tag

34 olylbenzoic acid (TBBA) was used as a chiral derivatization agent for the assignment of the absolute

35 ated electrospray deposition of liquid-phase derivatization agents as a means of on-tissue analyte de

36 ut derivatization, compared to methods using derivatization agents.

37 Random derivatization alters the properties of the bulk materia

38 rum GC-MS metabolomics in fruits using a new derivatization and a two dimensional peak deconvolution

39 s (including iodinated HAAs) by diazomethane derivatization and analysis using a GC-triple quadrupole

40 Moreover, post-synthetic derivatization and applications are presented.

41 The efficient derivatization and cleanup of alditol acetate derivative

42 While derivatization and complexation workflows have aimed to

43 " peptide to spike into the sample, of which derivatization and digestion benchmarks the quality of t

44 l includes 4 h of histone extraction, 3 h of derivatization and digestion, and only 1 min of MS analy

45 t by using 2,4-dinitrophenylhydrazine (DNPH) derivatization and electrospray ionization-tandem mass s

46 ine by ion-pair HPLC method with post-column derivatization and fluorimetric detection is proposed.

47 hich include pseudouridine-specific chemical derivatization and gas phase dissociation of RNA during

48 tent saponin adjuvants after simple chemical derivatization and identify VSA-2 (5b) as another MS-bas

49 acid epimerization were analyzed by chemical derivatization and LC-MS/MS.

50 tion reagent and parameters affecting in the derivatization and microextraction steps were studied an

51 Under optimum conditions of derivatization and microextraction steps, the method yie

52 The compositions of the derivatization and separation buffers were optimized to

53 the development and evaluation of a chemical derivatization and tandem mass spectrometry method that

54 w covers recent advances on the preparation, derivatization, and analysis of lipids.

55 paration that includes histone purification, derivatization, and digestion.

56 ly derivatized metabolites and chemicals for derivatization, and in a complete cell incubated medium.

57 ny specific UV/vis chromophores, no chemical derivatization, and no growth of suitable crystals.

58 croreactor; ii) "reversible" electrochemical derivatization; and iii) in situ mechanistic study and c

59 The presently developed NPB-based derivatization approach is expected to be essential in t

60 This derivatization approach overcomes the need for costly la

61 sis has been developed by combining a chiral derivatization approach with high-resolution trapped ion

62 yzed enyne cyclization reactions and further derivatization are described.

63 The optimum conditions of derivatization are: 500muL of isopropanolic eluate obtai

64 silylation techniques as well as in further derivatizations are also described.

65 provides numerous opportunities for further derivatization as well as site-selective functionalizati

66 cted by the C-3 fluorine stereochemistry and derivatization at C-5 and/or C-9 of the inhibitor.

67 ded the first analogs fit for human use, and derivatization at C12 led to 12-epi-pleuromutilins with

68 The rapid and robust diacetyl derivatization at low temperatures (e.g., -20 degrees C,

69 nge, we reported a universal, user-friendly, derivatization-based strategy, MELDI (mCPBA Epoxidation

70 alkyne-labeled 1c can be employed for toxin derivatization by click chemistry with an azide-containi

71 ore developed a linkage-specific sialic acid derivatization by dimethylamidation and subsequent amida

72 Direct access to 2-enoic acids allows derivatization by numerous approaches.

73 orward biomolecular attachment and multistep derivatization capabilities to these nanoparticles and,

74 Through strategic chemical derivatization, chiral chromatographic separation, and m

75 ificity was achieved by measuring E2 without derivatization, compared to methods using derivatization

76 pectral complexity is achieved by separation/derivatization, comprehensive molecular characterization

77 Derivatization conditions for 8 amines analysis were opt

78 aracterized using thermal desorption in situ derivatization coupled to a GCxGC-ToF/MS system.

79 yzed via a procedure based on injection-port derivatization coupled to gas chromatography-tandem mass

80 alkynes, SNO-OCTs contain multiple sites for derivatization, display stability under a variety of com

81 paration is a single-step protocol with high derivatization efficiency.

82 This 2-AA on-MALDI-target glycan derivatization eliminates tedious sample preparation and

83 The derivatization employs the inexpensive, environmentally

84 Critically, diacetyl derivatization enables the differentiation of discrete m

85 require many steps including extraction and derivatization ending in GC/MS or HPLC analysis, which m

86 The derivatization enhanced the ionisation efficiency of vit

87 tions, in vitro kinetic studies and chemical derivatization experiments, we characterize the interact

88 Derivatization, extraction and rinsing steps are not req

89 involves deproteination of milk followed by derivatization/extraction of the HAAs in the supernatant

90 Automated online derivatization facilitates to overcome the main obstacle

91 abolites in human plasma, based on precolumn derivatization followed by HPLC-inductively coupled plas

92 ratrol, along with glucose, were released by derivatization followed by reductive cleavage, a chemica

93 ol could be released from these lignins upon derivatization followed by reductive cleavage, a degrada

94 Polar metabolites were analyzed using derivatization followed by two-dimensional gas chromatog

95 products and pharmaceuticals, allowing rapid derivatization for investigation of structure-activity r

96 mycin and maduramicin directly without prior derivatization for the first time.

97 sotope-ratio mass spectrometry (LC-IRMS) and derivatization gas chromatography-IRMS (GC-IRMS), respec

98 mers and their transformation products using derivatization gas chromatography-mass spectrometry.

99 In this work, we refined a derivatization gas chromatography-mass spectroscopy tech

100 The amount of NaBPr4 added for derivatization has been found to be the main factor resp

101 membered rings, highlights the effects these derivatizations have on the macrocycle conformation, and

102 with phosphate buffer and sodium cyanide for derivatization in alkaline conditions.

103 we have developed on-tissue enzyme-assisted derivatization in combination with microliquid extractio

104 d using 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization in comparison with phenylhydrazine (PHN)

105 12) with no sample carryover and with inline derivatization in the case of poorly ionizing compounds.

106 In addition, by employing chiral derivatization in the sample preparation, the platform e

107 or modifications by late-stage semisynthetic derivatization in ways that enhance their biological pot

108 The validated method includes derivatization, in which FAs are sent through esterifica

109 harides were notably improved after chemical derivatization, in which signals were enhanced by 230-fo

110 urrent status of microbial BIA synthesis and derivatization, including rapid developments in the past

111 These results indicate FMOC derivatization is a suitable alternative for fumonisins

112 The derivatization is carried out on a 50-100 mg mineral sam

113 are known to show several derivatives, since derivatization is incomplete as well as highly matrix de

114 Derivatization is often required via chemical reaction.

115 Solid-phase extraction coupled with derivatization leads to selective identification of keto

116 Subsequent derivatization led to a fragment collection featuring re

117 C10 amide of lead compound 2 and subsequent derivatization led to novel carbon- and nitrogen-linked

118 The derivatization method enables one to identify various ca

119 A newly developed derivatization method revealed that the level of carbamo

120 come this problem, we developed an on-tissue derivatization method with Girard's reagent T (GirT) in

121 Herein, we described a sequential selective derivatization method, by which alpha-2,6- and alpha-2,3

122 ion as a special class of late-stage peptide derivatization method.

123 ate reader to analyze a chlorocoumarin amine derivatization methodology for the ee determination of a

124 However, previously developed on-fiber derivatization methods have been hindered by poor loadin

125 Solid phase microextraction (SPME) on-fiber derivatization methods have facilitated the achievement

126 We used sialic acid linkage-specific derivatization methods to improve separation and the lev

127 that cannot be determined accurately by DNPH derivatization methods.

128 zation techniques and stable isotope labeled derivatization methods.

129 In addition, we explored compatible sample derivatization methods.

130 <0.4 per mille was achieved by GC-IRMS after derivatization of >100 ng of DPC with 160-fold excess of

131 The late-stage derivatization of a bromine-substituted, tetracyclic ind

132 Here, we report the discovery and derivatization of a series of pyrazolo[3,4-b]pyridines t

133 of unnatural amino acids and the late-stage derivatization of a tripeptide.

134 atalytic step is employed for the asymmetric derivatization of alpha,beta-unsaturated aldehydes to 4,

135 ize enzymatic DNA repair, followed by direct derivatization of AP sites in the nuclear lysate with O-

136 the synthesis of -CD(3)-containing products, derivatization of bioactive molecules, and chemoselectiv

137 Derivatization of biologically active peptides by conjug

138 This research showed that in situ derivatization of cannabinoids through addition of an N-

139 ell-suited for the aza-functionalization and derivatization of complex organic molecules.

140 mophthalic anhydride (TBPA) was used for the derivatization of free NH2-groups.

141 We performed in situ derivatization of free thiol groups from thiol-containin

142 Without chemical derivatization of glycan samples, glycan visualization b

143 te, was used as a convenient reagent for the derivatization of histamine, tyramine, tryptamine and 2-

144 on the surface of HSA molecules due to prior derivatization of HSA molecules with appropriate functio

145 PAAC) enables high resolution 3-D photoclick derivatization of hydrogels and tissues.

146 eview provides a comprehensive survey of the derivatization of hydrogen-terminated, oxide-free silico

147 roduction, defined as purposeful biochemical derivatization of intended target molecules.

148 , we developed a straightforward approach by derivatization of MAGs with 3-nitrophenylboronic acid (3

149 -like small molecules, as well as late stage derivatization of natural compounds.

150 ource for the discovery of therapeutics, the derivatization of natural products has been extensively

151 Besides, synthetic application in both the derivatization of obtained diene products and the on-DNA

152 cycle toxin conjugate (BTC) was generated by derivatization of one of these Bicycles with the potent

153 ce a more flexible methodology for selective derivatization of phenolic moieties in complex biomolecu

154 chromatography in metabolomics, namely, the derivatization of polar metabolites prior to analysis.

155 r the synthesis of chlorins has entailed the derivatization of porphyrins.

156 gies for preparation of the ring and further derivatization of preformed oxetane-containing building

157 Building on our previously reported derivatization of resorcylate natural products to produc

158 ntageous over classical methods as clean up/ derivatization of samples is not required for formaldehy

159 thod to quantify SCFAs by coupling amidation derivatization of SCFAs with paper-loaded direct analysi

160 otocol is highlighted by its application for derivatization of several amine-containing drugs.

161 nal groups, as exemplified by the late-stage derivatization of several complex pharmaceutical compoun

162 ed pyrrolidines and indolines, without prior derivatization of the alcohol or solvolytic conditions.

163 derstanding of the mechanism underlying pEtN derivatization of the cellulose exopolysaccharide remain

164 this method is further demonstrated through derivatization of the chiral aziridine products to obtai

165 thine ylide, and dipolarophile), and further derivatization of the cycloadduct are reported.

166 Chemical derivatization of the hydrogenolysis products showed tha

167 Synthetic derivatization of the initial hit led to a more potent a

168 ve also been discovered, commonly leading to derivatization of the internal carbon atom.

169 Simple derivatization of the ligands yields larger spectral cha

170 These new routes allow for the derivatization of the methoxyphenyl and chlorophenyl rin

171 erse nucleophiles, as well as the late-stage derivatization of the natural product k252a.

172 cation was made possible through SuFEx click derivatization of the pendant sulfonyl fluoride group in

173 The derivatization of the phosphonic acid functionality of F

174 Decomplexation and further derivatization of the products provide access to several

175 n the preparation of mixed bis-triazoles and derivatization of the protein BSA with fluorescent dye a

176 f natural peptides, like Magainin 2, and the derivatization of the protein Ubiquitin.

177 hloric acid hydrolysis, followed by chemical derivatization of the released monomer sugars and quanti

178 blished via the nitrocatechol ring, allowing derivatization of the side chain for future lead optimiz

179 Reduction of the nitro group followed by derivatization of the so formed anilines 14 gave the N-a

180 Derivatization of the target amines required minimal sam

181 Chemical derivatization of these diterpenoids has demonstrated th

182 Derivatization of thiol-containing metabolites with CHC-

183 Derivatization of this intermediate affords lignans feat

184 Rational systematic derivatization of this scaffold to maximize Eis inhibiti

185 ol that enables a particular straightforward derivatization of unsaturated lipids in tissue sections.

186 trolled choices of highly diastereoselective derivatizations of 1.

187 The synthetic utility has been shown in derivatizations of complex bioactive compounds and an op

188 luorenyne core, which pave the way for novel derivatizations of motifs relevant to materials chemistr

189 desirable for enabling late-stage oxidative derivatizations of pharmaceuticals and medicinally impor

190 Facile derivatizations of the azidoamine group demonstrate the

191 Furthermore, two possible orthogonal derivatizations of the enantioenriched aldehydes were pe

192 hydroboration and subsequent stereospecific derivatizations of the secondary boronates.

193 method) and a total analysis time (including derivatization) of less than 15 min.

194 d, many of which rely on the use of chemical derivatization or molecular assemblies, resulting in UV/

195 available starting materials without needing derivatization or the use of protecting groups.

196 tive profiling of these metabolites, without derivatization or use of ion-pairing agents, are lacking

197 Such differences in SA derivatization, particularly O-acetylation, could have c

198 Prior to derivatization, plant ITCs were isolated and purified us

199 tudies were carried out using five different derivatizations possessing two NO2, five fluoro, two flu

200 id phase extraction chromatography, and FMOC-derivatization preceded analysis by reverse phase HPLC w

201 zinc in acidic solution, hydrophobization by derivatization, preconcentration on C18 cartridge, and L

202 es with guanidine avoiding its NH-protection/derivatization prerequisite for electronic modulation.

203 ed a cross-validation of the newly developed derivatization procedure and quantification based on mon

204 re, we present a sensitive and simple matrix derivatization procedure using propionic anhydride that

205 e enzymatic products during the trapping and derivatization processes.

206 Carboxylic acid derivatization produces a broad range of previously unkn

207 tabase can be rapidly modified to search for derivatization products, oxidation products, in-source f

208 mmol scale reaction, a sequential allylation/derivatization protocol and multiple examples of site-se

209 A practically simple derivatization protocol for determining the enantiopurit

210 A practically simple three-component chiral derivatization protocol has been developed to determine

211 tion, sample clean-up, preconcentration, and derivatization protocols.

212 ducts are amenable to a multitude of further derivatizations ranging from olefin chemistry to C-H act

213 The derivatization reaction was performed to pinpoint the do

214 ence of experimental parameters effective on derivatization reaction yield and extraction performance

215 d, and a correspondingly large collection of derivatization reactions are possible on the products, i

216 Three derivatization reactions have been carried out with N-me

217 is rationale has established that the use of derivatization reactions widens the scope of targeted mo

218 iophenes participate in a variety of in situ derivatization reactions, showcasing that these borylate

219 Xanthydrol was used as a derivatization reagent and parameters affecting in the d

220 reproducibility and standard lifetime due to derivatization reagent reactivity.

221 or a reactive, toxic, and otherwise unstable derivatization reagent standard.

222 tion/ionization and simultaneously serves as derivatization reagent that allows functionalization of

223 Here, we report the first derivatization reagent, (E)-2-cyano-N-(2-(2,5-dioxo-2,5-

224 E fibers can now be reproducibly loaded with derivatization reagent, from an easy to use and safe pla

225 opment of two novel pyridinium fixed-charged derivatization reagents that incorporate a photolabile a

226 In plasma, derivatization resulted in stable derivatives and increa

227 rmination in conjunction with a novel sample derivatization scheme in which glucose oxidase and lacta

228 yme immobilization procedure, and the online derivatization scheme, the available sampling frequency

229 In optimized reaction conditions, high derivatization selectivity (~99%) was achieved for both

230 Here, through extraction, derivatization, spectroscopy, chromatography, and mass s

231 y mass spectrometry with no prior separation/derivatization step has been developed.

232 nol in finfish using an off-line dansylation derivatization step to enhance signal intensity.

233 ectrometry detection (GC-TQ/MS) with a prior derivatization step with O-(2,3,4,5,6-pentafluorobenzyl)

234 ofile in mussel samples, avoiding a previous derivatization step, required when gas chromatographic a

235 tiplexed HRMS (MSX) analysis without using a derivatization step.

236 sed on permethylation in DMSO as the initial derivatization step.

237 The method requires no derivatization steps or sample extraction procedures tha

238 tion ionization (MALDI), typically requiring derivatization steps to overcome their low ionization ef

239 d does not require hydrolysis, extraction or derivatization steps, but rather a simple "dilute and sh

240 This application broadens the scope for derivatization strategies on tissue surfaces to elucidat

241 better functional group compatibility, novel derivatization strategies were developed.

242 xy Gdm, and thereby provides a new synthetic derivatization strategy and implicates a potential uniqu

243 Herein, an on-tissue chemical derivatization strategy based on positively charged hydr

244 Nevertheless, no derivatization strategy for free sulfhydryl groups in ti

245 three extracts by GC/MS using the silylation derivatization technique revealed 53 compounds, 17 of th

246 enhancement using diazomethane (TrEnDi) is a derivatization technique that significantly enhances the

247 mprove the accessibility and utility of this derivatization technique.

248 osite design was implemented to optimize the derivatization temperature and extraction time, which we

249 pid analysis platform by coupling online C=C derivatization through the Paterno-Buchi reaction with l

250 terms of optimal injection port temperature, derivatization time and sample: N-methyl-N-(trimethylsil

251 ring high resolving power instrumentation or derivatization to circumvent this issue.

252 ucleosides and nucleotides generally require derivatization to induce gelation, guanosine and its cor

253 migration, a facile low temperature diacetyl derivatization to stabilize regiospecificity, and tandem

254 Natural water experiments, including derivatization to transform primary and secondary amines

255 The derivatization treatment leads to characteristic band sh

256 6%, 346.6% as measured by o-phthaldialdehyde derivatization, trichloroacetic acid precipitation and h

257 After 4-methyl-1,2-benzoquinone derivatization, UHPLC-Q-ToF MS analyses specifically ide

258 ation agents as a means of on-tissue analyte derivatization using 2-picolylamine; we were able to det

259 (MSI) is combined with an on-tissue chemical derivatization using diethyldithiocarbamate (DDTC).

260 lcohols at room temperature with no chemical derivatization using Mg(II)bisporphyrin as a host.

261 and G1 was enhanced by post-column chemical derivatization using pyridinium bromide perbromide.

262 e mosquito body was conducted without sample derivatization using selected reaction monitoring of mas

263 The most convenient method seems to be derivatization via direct metalation, but synthesis of p

264 a GC-MS injector to simulate on line in situ derivatization-volatilization-transfer steps.

265 Complete derivatization was achieved in minutes without overoxidi

266 Furthermore, on-tissue derivatization was applied for MALDI-MSI of N-glycans fr

267 Manual and in situ derivatization was assayed and the chromatographic respo

268 The derivatization was carried out at 45 degrees C in 15 min

269 HS-SPME coupled with in-situ derivatization was more straightforward (three steps) an

270 Furthermore, in-port derivatization was optimized in terms of optimal injecti

271 Sites selected for Alexa 488 (C5) derivatization were in the alpha-helical domain (residue

272 lently premixed with d(0)-NPB to perform MAG derivatization, which resulted in rapid identification o

273 bstitution of ETP alkaloids offers versatile derivatization while maintaining potent anticancer activ

274 matography system was used for inline chiral derivatization with (S)-naproxen chloride and subsequent

275 (CE-LIF) method that analyzes Ado and Ino by derivatization with 2,4,6-trinitrobenzenesulfonic acid t

276 y been detected spectrophotometrically after derivatization with 2,4-dinitrophenylhydrazine (DNPH) to

277 re improved through ion/ion-mediated peptide derivatization with 4-formyl-1,3-benezenedisulfonic acid

278 ds in human plasma and serum using precolumn derivatization with 6-aminoquinolyl-N-hydroxysuccinimidy

279 Pre-column derivatization with 9-fluorenylmethyl chloroformate (FMO

280 a solid-phase extraction method followed by derivatization with a charged tag to characterize ketone

281 was established, after total hydrolysis, by derivatization with a new chiral reagent, (5-fluoro-2,4-

282 Reactions involving hydroxyl group derivatization with a variety of electrophilic reagents

283 The specific detection via derivatization with an ethanolic potassium hydroxide sol

284 ) is presented, and its subsequent selective derivatization with Bronsted acids, MeOTf, Ph2ECl (E = P

285 physically separate both enantiomers, a SPG derivatization with camphorsulfonic acid and Mosher's ac

286 :25% ammonium hydroxide (70:30:4:2 v/v/v/v), derivatization with Carr-Price reagent and video densito

287 A new and sensitive pre-column derivatization with dabsyl chloride followed by dispersi

288 Derivatization with dabsyl chloride leads to improving s

289 tone using a tissue homogenizer, followed by derivatization with dansyl chloride and analysis by liqu

290 % ammonium hydroxide (75:15:10:1.6 v/v/v/v), derivatization with Dragendorff reagent and scanning den

291 Here, derivatization with Girard T reagent was used to charge-

292 13)C-isotope labeling of polyphenols through derivatization with isotopic dansyl chloride reagents an

293 and alpha2,6-linked sialic acids by covalent derivatization with methylamine.

294 ocyanates (ITCs) determination that involves derivatization with N-acetyl-l-cysteine (NAC) and separa

295 alencia sweet orange (Citrus sinensis) after derivatization with N-methyl-N-trimethylsilyltrifluoroac

296 After its derivatization with ortho-phenylenediamine, we analyzed

297 as achieved by forming diastereomers through derivatization with the chiral agent (+)-1-(9-fluorenyl)

298 ent tryptic digestion and N-terminal peptide derivatization with the ultraviolet chromophore AMCA yie

299 need for pre-functionalization or substrate derivatization, would therefore be valuable.

300 The derivatization yield was higher than 90%, and it could t