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1 and is consistent with a significant primary deuterium isotope effect.
2  dehydrogenation reaction exhibited the same deuterium isotope effect.
3  dependence, and a larger than usual upfield deuterium isotope effect.
4 inding specificity was assessed by using the deuterium isotope effect.
5 alysis and inhibition, we determined solvent deuterium isotope effects.
6  postulated on the basis of spectroscopy and deuterium isotope effects.
7 sidered unlikely given the lack of a solvent deuterium isotope effect above the breakpoint in the pH
8  Ru and zero order in phosphine, and kinetic deuterium isotope effects all point to a mechanism invol
9                                      Kinetic deuterium isotope effects along with K(m) values permitt
10           The non-competitive intramolecular deuterium isotope effect, an estimate of the intrinsic i
11 r, the observation in N694C of a significant deuterium isotope effect, anaerobic reduction of iron by
12                       Intramolecular kinetic deuterium isotope effect and (18)O labeling experiments
13 .0064 (assuming 5.7 as the intrinsic primary deuterium isotope effect and 1.054 as the product of the
14                           Results on kinetic deuterium isotope effect and quenching studies are in co
15 ental steps were associated with an hydrogen/deuterium isotope effect and that glycolate alpha-deprot
16                An analysis of noncompetitive deuterium isotope effects and competitive tritium isotop
17                                      Solvent deuterium isotope effects and maps of the electrostatic
18                     Furthermore, comparative deuterium isotope effects and the relative rates of inac
19 values of the theoretically relevant kinetic deuterium-isotope effect and its dependence on temperatu
20  decreases in activity, the measured solvent deuterium isotope effects, and changes in the pH depende
21 ith the NADPH-supported P450 reactions, high deuterium isotope effects ( approximately 7) were seen i
22 while a small pH-independent primary kinetic deuterium isotope effect (approximately 1.3) is observed
23                           Because of a large deuterium isotope effect, approximately 7, the quantum a
24   For Mg(2+)-assisted reactions, the solvent deuterium isotope effects are 1.23 and 0.25 for general
25              Values of substrate and solvent deuterium isotope effects are consistent with the kineti
26     Furthermore, we demonstrate that solvent deuterium isotope effects are involved in the thermal de
27                                The enzymatic deuterium isotope effects are lower by a factor of 2, bu
28                                         V/Km deuterium isotope effects are observed for both substrat
29                                  Significant deuterium isotope effects are obtained in these reaction
30 cal shifts, a Steiner-Limbach correlation, a deuterium isotope effect as well as quantitative values
31                           Apparent intrinsic deuterium isotope effects as high as 15 were measured.
32 ed analysis of the pH dependence and solvent deuterium isotope effects associated with the reaction u
33 onsistent with a stepwise mechanism with the deuterium isotope effect at C3 being only on the decarbo
34                       The observed 'inverse' deuterium isotope effect at pH < 8 can be explained by a
35                                    Secondary deuterium isotope effects at C-3 were 2.5% at pH 7 and 3
36                                              Deuterium isotope effects at C2 of aspartate and heavy a
37                                   With NADP, deuterium isotope effects at C3 of 1.17 and 1.08 for di-
38 ean lipoxygenase 1 have indicated very large deuterium isotope effects, but have not been able to dis
39                    According to a pronounced deuterium isotope effect (CH3OD), this motion of heavy a
40  reduced values with deuterium substitution (deuterium isotope effect) characteristic of MAO B.
41          This relatively high intramolecular deuterium isotope effect confirmed the initial hydrogen
42                                          The deuterium isotope effects (D)(V/K) for (4R)-[4-(2)H]-NAD
43                                          The deuterium isotope effects (D)V and (D)(V/K) for (4R)-[4-
44 kylation of DMN had a high intrinsic kinetic deuterium isotope effect ((D)k(app) approximately 10), w
45 method yielded an apparent intrinsic kinetic deuterium isotope effect ((D)k) of 15.
46 tions of the intrinsic primary and secondary deuterium isotope effects ((D)k = 2.7, (alpha)(-D)k = 1.
47                                      Primary deuterium isotope effect data were measured for the wild
48                               Solvent pH and deuterium isotope-effect data are also used to evaluate
49                                              Deuterium isotope effects demonstrate that solvent proto
50                        The intrinsic primary deuterium isotope effect determined from single-waveleng
51                A trend of decreasing kinetic deuterium isotope effect for E225I > wild-type > mutant
52                        The pH dependence and deuterium isotope effect for reduction of isolated compo
53                              A large kinetic deuterium isotope effect for the amine activation proces
54                                          The deuterium isotope effect for the reaction was determined
55 to solvent accessibility by detection of the deuterium isotope effect for Y(Z) oxidation and by 2H ES
56 ly fast in the catalytic cycle; high kinetic deuterium isotope effects for all four lauric acid hydro
57  In contrast, significant suppression of the deuterium isotope effects for CYP2B1 occurred only with
58                                 (ii) Solvent deuterium isotope effects for hydrolysis of Z-Gln-Gly by
59            Inter- and intramolecular kinetic deuterium isotope effects for phenacetin O-deethylation
60                               Intramolecular deuterium isotope effects for the benzylic hydroxylation
61 )/k(D) = 9.7 and 6.8, respectively), whereas deuterium isotope effects for the naphthyl and biphenyl
62 type of KR is found to exhibit a significant deuterium isotope effect (for 9 vs d(1)-9).
63 r oxidation of malate, while the equilibrium deuterium isotope effect from deuteration at C-2 of the
64   Noncompetitive measurements of the primary deuterium isotope effect give a value of ca. 40 which is
65 , high noncompetitive intermolecular kinetic deuterium isotope effects (&gt;/= 5.5) were observed for al
66                       In addition, a solvent deuterium isotope effect has been taken as evidence agai
67 2-(2)H4]choline ((18)F-D4-FCH), based on the deuterium isotope effect, has been developed.
68               Kinetic parameters and primary deuterium isotope effects have been determined for wild-
69 er conditions of catalytic turnover, kinetic deuterium isotope effects have been measured as a functi
70              15N isotope effects and solvent deuterium isotope effects have been measured for the hyd
71 ms and in the P-O(R) ester bond, and solvent deuterium isotope effects, have been measured for the hy
72                                    Secondary deuterium isotope effects (IEs) on acidities have been a
73                                    Secondary deuterium isotope effects (IEs) on basicities of isotopo
74 liable temperature dependence of the kinetic deuterium isotope effect in a 1,5-hydrogen shift, the ti
75                                     From the deuterium isotope effect in the FVP of PhCD(2)CH(2)OPh,
76                                  The overall deuterium isotope effect in the presence of AdoCbi-GDP (
77        Here, we show that the conformational deuterium isotope effect, in combination with Saunders'
78                             Modest substrate deuterium isotope effects indicate that hydride transfer
79 lack of significant kinetic and partitioning deuterium isotope effects indicates that the isomerizati
80 ind that the magnitude of the conformational deuterium isotope effect is 252.1, 28.3, and 7.1 J/mol (
81        However, the magnitude of a substrate deuterium isotope effect is almost identical for wild-ty
82                          The solvent kinetic deuterium isotope effect is also unity at low AcCoA, but
83 e results it is estimated that the aldehydic deuterium isotope effect is approximately 1.9 after form
84   With TEMPOH as sacrificial H atom donor, a deuterium isotope effect is observed (k(H)/k(D) = 3.5),
85                                    A solvent deuterium isotope effect is observed for the azide-accel
86               Remarkably, a primary hydrogen-deuterium isotope effect is readily detected at the sing
87         This study demonstrates that (a) the deuterium isotope effect is useful in assessing the bind
88                              A large primary deuterium isotope effect (k(H)/k(D) = 18.9 at 295 K) ind
89 traction mechanism, in line with the kinetic deuterium isotope effects, k(H)/k(D), of 2.0 and 3.1 mea
90                    These experiments yield a deuterium isotope effect, kH/kD approximately 3 for ABLM
91            Here we report results of kinetic deuterium isotope effect (KIE) measurements on ETp throu
92 CHO showed a high apparent intrinsic kinetic deuterium isotope effect (KIE), >/=8.
93                    On the basis of (13)C and deuterium isotope effects, L-ribulose-5-phosphate 4-epim
94                                  Theoretical deuterium isotope effects match well with those from exp
95  microM, respectively, and a primary kinetic deuterium isotope effect of 1.3 and 1.8 on V/ K AcCoA an
96                               Furthermore, a deuterium isotope effect of 1.9 and a linear proton inve
97 )]farnesylcysteine as a substrate, a primary deuterium isotope effect of 2 was observed on the steady
98 of a pL (L being H or D)-independent solvent deuterium isotope effect of 2.
99                            An intermolecular deuterium isotope effect of 2.0-2.5 was observed under s
100  respectively, giving a calculated intrinsic deuterium isotope effect of 3.3 +/- 0.9, consistent with
101 eavage of the oxo linkage exhibits a solvent deuterium isotope effect of 3.6, but a similar effect is
102                                  A substrate deuterium isotope effect of 32 was measured for the k(ca
103 icroM, respectively, while a primary kinetic deuterium isotope effect of about 1.4 was obtained on V,
104                In the steady-state a solvent deuterium isotope effect of about 2 was measured on (V/K
105 ocus on determining if the unusual aldehydic deuterium isotope effect of approximately 1.5 observed i
106  is asynchronous, however, with an intrinsic deuterium isotope effect of approximately 5 and a 13C is
107                                    A solvent deuterium isotope effect of three to seven was observed
108                   Reaction of CD3O* showed a deuterium isotope effect of ~6.5.
109                                      Smaller deuterium isotope effects of 1.03-1.04 from dideuteratio
110            Using 1D (13)C NMR, we have found deuterium isotope effects of 1.043 +/- 0.004, 1.027 +/-
111                                      Solvent deuterium isotope effects of 1.3 and 2.6 on V/K and V(ma
112                       Noncompetitive kinetic deuterium isotope effects of 2-3 were measured for all O
113               Intermolecular non-competitive deuterium isotope effects of 3.1-3.8 were measured for k
114                               Normal primary deuterium isotope effects of 5.0(1.2) and 6.0(2.0) in pa
115 omparison of the pH profiles and the solvent deuterium isotope effects of A. thaliana GS and the Arg-
116 50) 2E1 substrates are known to show kinetic deuterium isotope effects of approximately 5 on Km (DK =
117           d(3)-Cholesterol showed no kinetic deuterium isotope effect on C-22, indicating that C-H bo
118 SB hydrolysis in the dark state and a strong deuterium isotope effect on dark state SB hydrolysis.
119               There was no selective solvent deuterium isotope effect on enzyme catalysis.
120 ansfer and the presence of a kinetic solvent deuterium isotope effect on hydride transfer.
121 n is the finding that [3-(2)H]-10 exhibits a deuterium isotope effect on inactivation of 3.3, suggest
122                              A small primary deuterium isotope effect on k(cat) (1.5) and a slightly
123                                  The solvent deuterium isotope effect on k(cat) is 2.7 +/- 0.2 and 1.
124                      A small solvent kinetic deuterium isotope effect on k(cat) of 1.76 +/- 0.25, ind
125 the origin of the relatively small substrate deuterium isotope effect on k(cat)/K(m)(O(2)).
126                                  The primary deuterium isotope effect on K1 for flavin reduction at h
127                                  The primary deuterium isotope effect on Kcat for cytochrome c reduct
128                     The absence of a solvent deuterium isotope effect on product distribution in the
129                                          The deuterium isotope effect on the consumption of [1-2H,1,2
130                           An analysis of the deuterium isotope effect on the two rapid-mix reaction s
131  oxidation and reduction rates for Y(Z), the deuterium isotope effect on these rates, and the Y(Z)* -
132                                          The deuterium isotope effect on this hydrogen bridge is 2.2
133 , lungs, kidneys, and spleen showed a robust deuterium isotope effect on uptake, IQ, k3, and lambdak3
134                                          The deuterium isotope effect on V/K(aspartate) is pH indepen
135                               Secondary beta deuterium isotope effects on acidity constants of ammoni
136                               Secondary beta-deuterium isotope effects on amine basicities are measur
137                                      Solvent deuterium isotope effects on binding were observed for b
138  High intermolecular non-competitive kinetic deuterium isotope effects on both kcat and kcat/Km, from
139                                          The deuterium isotope effects on k(cat) and k(cat)/K(m) in n
140                          Significant primary deuterium isotope effects on kcat (Dkcat) and kcat/KPt (
141                          Analysis of solvent deuterium isotope effects on NCS-chrom degradation and D
142                                              Deuterium isotope effects on nitrogen and proton are of
143 imental and theoretical investigation of the deuterium isotope effects on the bacterial luciferase re
144                        Interestingly, double-deuterium isotope effects on the Cys130Ser mutant also s
145 hy, (1)H NMR, site-directed mutagenesis, and deuterium isotope effects on the geometry and chemical s
146 d to explore the active site topography; and deuterium isotope effects on the hydrogen atom abstracti
147     Primary deuterium [NADPH(D)] and solvent deuterium isotope effects on the kinetic parameters were
148                                  The primary deuterium isotope effects on the V(max) and the V/K(lact
149   (R)-NADPD but not (S)-NADPD showed kinetic deuterium isotope effects on V and V/K of about 1.9 and
150                                Large primary deuterium isotope effects on V and V/K using 3-APADPH in
151                               Larger primary deuterium isotope effects on V and V/K were observed for
152                                      Solvent deuterium isotope effects on V and V/K(MgHIc) were near
153 th substrate and coenzyme, together with the deuterium isotope effects on Vmax and Vmax/Km, have been
154                                      Also, a deuterium-isotope effect on the burst rate constant of p
155                                  The primary deuterium-isotope effect on V/K6PG for both enzymes is c
156                                              Deuterium-isotope effects on V, V/KNADP, and V/K6PG are
157 d by solvation changes that generate solvent deuterium isotope effects originating from hydrogen ion
158 tly different isotope scrambling and kinetic deuterium isotope effect patterns.
159                                    A product deuterium isotope effect (PIE) of 1.0 was determined as
160             A similar experiment involving a deuterium isotope effect previously returned the same vo
161     The combination of solvent and substrate deuterium isotope effects rules out solvent deuterium ex
162                                      Solvent deuterium isotope effect studies indicate that transfer
163 uted tetrahydropyridines, we have undertaken deuterium isotope effect studies on the substrate and in
164                        Viscosity and solvent deuterium isotope effects studies suggest the isomerizat
165                                          The deuterium isotope effect study suggested that substituti
166 howed a pH 6 activity maximum but no kinetic deuterium isotope effect, suggesting protons are not tra
167 o acetaldehyde is characterized by a kinetic deuterium isotope effect that increases K(m) with no eff
168          Catalysis is modulated by a kinetic deuterium isotope effect that reduces the overall interc
169 00Lys mutant does not exhibit the very large deuterium isotope effects that are observed for homolysi
170 er, pressure increases the primary intrinsic deuterium isotope effect, the opposite of what was obser
171 C-clorgyline ((11)C-clorgyline-D2) using the deuterium isotope effect to assess binding specificity.
172 IE results and the lack of a kinetic solvent deuterium isotope effect together provide strong evidenc
173                                              Deuterium isotope effects, typical of a concerted metala
174                                  The primary deuterium isotope effect using L-serine 2-D is one on (V
175 , (iv) the lack of a non-competitive kinetic deuterium isotope effect, (v) the lack of a kinetic burs
176 atter compound, a substantial intermolecular deuterium isotope effect was observed for N-demethylatio
177                                     A normal deuterium isotope effect was observed for the hydrogenol
178     A prominent secondary four-bond hydrogen/deuterium isotope effect was observed from proton NMR at
179                                 A pronounced deuterium isotope effect was observed in alternating d(G
180                                    A primary deuterium isotope effect was observed under single-turno
181  homoisocitrate as the substrate, no primary deuterium isotope effect was observed, and a small (13)C
182 % of that for the wild-type complex, and the deuterium isotope effect was significantly decreased.
183 he solvent, primary, secondary, and multiple deuterium isotope effects were most consistent with a ch
184 p is not rate-limiting, while larger primary deuterium isotope effects were observed for poor ketoaci
185 rs at 107.5 +/- 3 s-1 and exhibits a 10-fold deuterium isotope effect when (4R)-[2H]NADH is substitut
186 k of a kinetic burst, and (vi) the lack of a deuterium isotope effect when the reaction was initiated
187 ments also indicate that there is no primary deuterium isotope effect with L-serine 2-D.
188  mutations increase the value of the primary deuterium isotope effect with tryptophan as a substrate,
189 re observed in the magnitudes of the primary deuterium isotope effects with NADPD, consistent with de

 
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