ライフサイエンスコーパス: digitonin

1 -3 by surface membrane permeabilization with digitonin.

2 assayed in situ after permeabilization with digitonin.

3 ved after permeabilization of the cells with digitonin.

4 embranes were selectively permeabilized with digitonin.

5 ibroblasts that have been permeabilized with digitonin.

6 nophore combinations (nigericin and CCCP) or digitonin.

7 orescence microscopy and catalase release by digitonin.

8 abilization of membrane proteins by CHAPS or digitonin.

9 DNA synthesis induced by another detergent, digitonin.

10 in B or nonselectively to small solutes with digitonin.

11 xtractions based on the weak ionic detergent digitonin.

12 ry alphabeta T cells after solubilization in digitonin.

13 eased from infected cells permeabilized with digitonin.

14 and SDS-lysed cells or from cells lysed with digitonin.

15 entrifugation and membrane solubilization by digitonin.

16 assayed in situ after permeabilization with digitonin.

17 ed hypersensitivity of the outer membrane to digitonin.

18 epithelial cells (HLE-B3) permeabilized with digitonin.

19 After permeabilization with digitonin, 50% of total glucokinase remained bound intra

20 dysenteriae with different concentrations of digitonin, a steroid glycoside that interacts with chole

21 On-column treatment with digitonin allowed for the separation of the plasma membr

22 ormational change is blocked in solutions of digitonin, although changes in optical absorbance accomp

23 complex by using the nondenaturing detergent digitonin and a one-step immunoaffinity technique.

24 lasma membrane permeabilizing agents such as digitonin and acetone/methanol, while cholesterol deplet

25 mbrane-permeable cholesterol-binding agents (digitonin and nystatin), but not the lipid-binding agent

26 the exported complexes by being stable in 1% digitonin and unstable in 1% Nonidet P-40.

27 face in solutions of dodecyl maltoside (DM), digitonin, and phospholipid bilayers of two compositions

28 The nonionic detergent, digitonin, and the anionic detergent, sodium dodecyl sul

29 s in state II, whose binding is sensitive to digitonin, and which are absent in DeltaLhca4.

30 Treating cells with digitonin at 5 micrograms/ml permeabilizes the plasma me

31 -treated 143B.TK(-) cells permeabilized with digitonin at a concentration not affecting the mitochond

32 ddress the intracellular functions of p22, a digitonin-based "bulk microinjection" assay was develope

33 Two of these compounds, amiodarone and digitonin, can act synergistically with all-trans-retina

34 rin from Bacillus subtilis QST713 as well as digitonin, CHAPS, and lysophosphatidylcholine solubilize

35 The sterol-containing agent digitonin completely inactivated IPC synthase.

36 conditions that differed in temperature and digitonin concentration (200-1200 ug/ml), we consistentl

37 C and 800 ug/ml digitonin, i.e., the lowest digitonin concentration that extracted cytosolic GAPDH t

38 nsition pore, whereas dimers obtained at low digitonin concentrations from the Arg-8 variants display

39 ear-atomic resolution structures resolved in digitonin consistent with all principle functional state

40 cells that were fixed and permeabilized with digitonin, demonstrating that they were not membrane bou

41 Cells are permeabilized using saponin (SAP), digitonin (DIG) or recombinant perfringolysin O (rPFO) (

42 ingle cell trimodal omics measurements after digitonin (DIG) permeabilization were generally better t

43 el electrophoresis in the presence of 0.003% digitonin displayed a supercomplex composed of homodimer

44 ria that may have been also permeabilized by digitonin do not contribute to ATP consumption by the ex

45 DTrc8 expression increased the level of digitonin-extractable CSN complex, consistent with eleva

46 evidence for specific binding of cytoplasmic digitonin-extractable glucokinase.

47 g (gGlu-83) with Ala or Lys destabilized the digitonin-extracted F-ATP synthase, resulting in decreas

48 icle sorting and isolated cytosolic mRNAs by digitonin extraction.

49 er photolysis measurements were conducted on digitonin extracts of artificial pigments prepared from

50 Digitonin extracts of mitochondria from cardiolipin-cont

51 es of similar composition were identified in digitonin extracts of ribosome-free membranes, indicatin

52 Using digitonin fractionation and fluorescence microscopy of F

53 Mis-localisation was confirmed by digitonin fractionation experiments.

54 e purification of PSII and PSI combined with digitonin fractionation of thylakoid membranes indicated

55 p proteins by tyeA mutant yersiniae with the digitonin fractionation technique.

56 Using immunoblotting, digitonin fractionation, immunofluorescence, and electro

57 hicken stomach membranes were solubilized in digitonin, glycoproteins were separated from solubilized

58 ells, indicated that the cells responding to digitonin had already been primed for apoptosis, and tha

59 hat purified active human gamma-secretase in digitonin has a total molecular mass of approximately 23

60 most relevant conditions (37 C and 800 ug/ml digitonin, i.e., the lowest digitonin concentration that

61 permeabilization of the plasma membrane with digitonin; (ii) insertion of Factor Xa cleavage sites; (

62 Use of the mild detergent digitonin in immunofluorescence also allowed centrin 2 t

63 es of complexes solubilized in the detergent digitonin, in which bivalency was not observed.

64 s retained after treatment of the cells with digitonin, indicating that it may interact with a membra

65 n, the majority of the transfected beta1D is digitonin-insoluble and is strongly associated with the

66 lack of membrane association and revealed a digitonin-insoluble nuclear fraction of S100A2, which wa

67 ain, Cys(39) and Cys(40), localized Syt11 to digitonin-insoluble portions of intracellular membranes

68 ither 10 microM amphotericin B or 10 micro M digitonin led to an aqueous-to-serosa-positive ISC.

69 n the plasma membrane was permeabilized with digitonin or in membrane preparations but not in intact

70 e permeabilization of transfected cells with digitonin or Triton X-100, respectively.

71 ere permeabilized without detergents such as digitonin or Triton X-100.

72 pt the mitochondrial outer membrane, such as digitonin, or maintain outer membrane exchange of adenin

73 Our enzymatic assay suggests that digitonin- or DDM-solubilized enzyme has the most PS syn

74 ral/centrilobular hepatocytes isolated using digitonin perfusion and on hepatic tissue using RNAscope

75 estruction of PP or PV sites was achieved by digitonin perfusion into the portal or inferior vena cav

76 After digitonin permeabilization of these cells, we found that

77 Cells depleted of ARF and Rho by digitonin permeabilization showed a significant reductio

78 nt study, submitochondrial fractionation and digitonin permeabilization studies indicated that both c

79 ods (detergent lysis, Dounce homogenization, digitonin permeabilization, and sonication) and three pr

80 s abolished Ca2+ -evoked secretion following digitonin permeabilization, compared with partial inhibi

81 ined to be exclusively within peroxisomes by digitonin permeabilization, immunofluorescence, protease

82 Interestingly, we found that digitonin permeabilization, which selectively releases s

83 Cytosolic calnuc was released by mild digitonin permeabilization.

84 Ran, which is released from cells during the digitonin permeabilization.

85 Digitonin-permeabilization studies of SINC-GFP-transfect

86 glucokinase overexpression combined with the digitonin-permeabilization technique.

87 es not stimulate nuclear protein import in a digitonin permeabilized cell assay and also inhibits wil

88 , that exhibited unexpected behavior both in digitonin-permeabilized and microinjected mammalian cell

89 nylenediamine as substrate, oxygen uptake by digitonin-permeabilized apoptotic mitochondria was great

90 is nucleotide inhibited cGMP accumulation in digitonin-permeabilized Caco-2 human colon carcinoma cel

91 se peptides for nuclear import activity in a digitonin-permeabilized cell assay.

92 Using digitonin-permeabilized cell assays to measure nuclear i

93 In a digitonin-permeabilized cell docking assay, RanBP1 stabi

94 Using a digitonin-permeabilized cell in vitro nuclear import sys

95 By using the digitonin-permeabilized cell system for protein import i

96 e requirements for plasmid nuclear import, a digitonin-permeabilized cell system was adapted to follo

97 lasmic fibers of the nuclear pore complex in digitonin-permeabilized cells and RanBP1 competition con

98 ity to stimulate the nuclear export of GR in digitonin-permeabilized cells and that the inhibition is

99 nses elicited from populations of intact and digitonin-permeabilized cells as well as from cells unde

100 Pax6 by Kap13 was shown to occur by using a digitonin-permeabilized cells assay.

101 in malate/glutamate-dependent respiration in digitonin-permeabilized cells by approximately 90% and a

102 1) and was also imported into the nucleus in digitonin-permeabilized cells by these factors.

103 th the binding data, nuclear import of L2 in digitonin-permeabilized cells could be mediated by eithe

104 mbly assay using purified COPII proteins and digitonin-permeabilized cells has been applied to demons

105 Similarly, KCN titration assays on digitonin-permeabilized cells have revealed a COX capaci

106 r nuclear import on a non-nuclear protein in digitonin-permeabilized cells in a temperature- and ATP-

107 Nuclear import in digitonin-permeabilized cells in the absence of added fa

108 at the nuclear envelope (NE) was observed in digitonin-permeabilized cells in the absence of cytosoli

109 hovanadate (Na(3)VO(4)) to medium containing digitonin-permeabilized cells inhibits all ADP-ATP-using

110 wever, mitochondrial respiration measured in digitonin-permeabilized cells is impaired in unstable cl

111 Nuclear export of GFP-NFAT in digitonin-permeabilized cells occurs in a temperature- a

112 ies with RanGDP-conjugated colloidal gold in digitonin-permeabilized cells showed a large number of R

113 We have used digitonin-permeabilized cells to examine in vitro nuclea

114 uclear accumulation of the conjugated DNA in digitonin-permeabilized cells via the classical pathway

115 ous nuclear karyopherin alpha from nuclei of digitonin-permeabilized cells was quantitatively monitor

116 lucokinase and of phosphoglucoisomerase from digitonin-permeabilized cells was slower when cells were

117 import of HIV-1 pre-integration complexes in digitonin-permeabilized cells was used to demonstrate th

118 In digitonin-permeabilized cells, beta2 was able to dock A1

119 s reacted with the biotinylating reagents in digitonin-permeabilized cells, demonstrating that the ab

120 Using digitonin-permeabilized cells, it was shown that the tra

121 In digitonin-permeabilized cells, Kap beta2B mediates TAP-G

122 lassical nuclear localization signal, and in digitonin-permeabilized cells, nuclear accumulation of J

123 Addition of IP3 to digitonin-permeabilized cells, or agonist treatment of i

124 In digitonin-permeabilized cells, the Kap but not Crm1 stim

125 genase-dependent respiration, as measured in digitonin-permeabilized cells, was specifically decrease

126 Using digitonin-permeabilized cells, we show that SMN import d

127 The linking reaction was also shown in digitonin-permeabilized cells, with UDP-N-acetylglucosam

128 ort the import of pA-RCC1 into the nuclei of digitonin-permeabilized cells.

129 TF2 can stimulate the accumulation of Ran in digitonin-permeabilized cells.

130 ion signal (NLS)-dependent protein import in digitonin-permeabilized cells.

131 ersion and induced nuclear import of NEMO in digitonin-permeabilized cells.

132 oorly with [3H]myristate or [3H]stearate) in digitonin-permeabilized cells.

133 y was studied using pulse-chase labeling and digitonin-permeabilized cells.

134 ycocyanin (APC) in standard import assays in digitonin-permeabilized cells.

135 TP) activity in isolated mitochondria and in digitonin-permeabilized cells.

136 luorescence recovery after photobleaching in digitonin-permeabilized cells.

137 adhesive and lateral dimers was observed in digitonin-permeabilized cells.

138 nsulin receptor in a cell-free system and in digitonin-permeabilized Chinese hamster ovary (CHO) cell

139 e epidermal growth factor (EGF) receptors in digitonin-permeabilized CHO cells that overexpress human

140 % of the ATP-dependent secretion observed in digitonin-permeabilized chromaffin cells.

141 80 was efficiently imported in the nuclei of digitonin-permeabilized COS-7 cells in the presence of r

142 Analyses of cathepsin B and D activities in digitonin-permeabilized cultures and the monitoring of c

143 e been directly compared after expression in digitonin-permeabilized fibroblastic (CV-1) cells, provi

144 ochondrial Ca2+ uptake capacity using either digitonin-permeabilized GT1-7 neural cells or isolated G

145 ier to gene transfer, was undertaken using a digitonin-permeabilized HeLa cell assay.

146 was effectively imported into the nucleus of digitonin-permeabilized HeLa cells after incubation with

147 uclear import of the HPV16 E6 oncoprotein in digitonin-permeabilized HeLa cells could be mediated by

148 ed E1 protein failed to enter the nucleus of digitonin-permeabilized HeLa cells in vitro.

149 In vitro import assays in digitonin-permeabilized HeLa cells reveal that ORF29p is

150 When digitonin-permeabilized HeLa cells were incubated with H

151 streptavidin is imported into the nuclei of digitonin-permeabilized HeLa cells.

152 mic determinants of pDNA nuclear import into digitonin-permeabilized HeLa cells.

153 d the nuclear import pathways of HPV16 E6 in digitonin-permeabilized HeLa cells.

154 d nuclear uptake of fluorescent substrate in digitonin-permeabilized HeLa cells.

155 in the mitochondrial intermembrane space of digitonin-permeabilized hepatocytes, indicative of VDAC

156 tion assays of apolipoprotein B100 (apoB) in digitonin-permeabilized HepG2 cells indicated that multi

157 were concentrated in perinuclear regions of digitonin-permeabilized HepG2 cells.

158 We used digitonin-permeabilized human cells to analyze the mecha

159 tivity of anti-7A6 was observed in normal or digitonin-permeabilized human peripheral blood lymphocyt

160 dk2/cyclin E and Cdc2/cyclin B1 complexes in digitonin-permeabilized mammalian cells and also examine

161 02) inhibited NE uptake in intact as well as digitonin-permeabilized PC12 cells, but had no effect on

162 NE uptake into large dense core vesicles in digitonin-permeabilized PC12 cells, which indicates that

163 e-8 or caspase-3 stimulates OPA1 cleavage in digitonin-permeabilized rat brain mitochondria, suggesti

164 Furthermore, cell-free incubation of digitonin-permeabilized TC7 cells with the nido-OPDs res

165 In addition, a digitonin-permeabilized, cell-free transport in vitro as

166 t to restore agonist-induced PLD activity to digitonin-permeabilized, cytoplasm-depleted cells.

167 In addition, both intact and digitonin-permeablized COS-7 cells transfected with CD39

168 SOAT2-CHO cells prepared by a treatment with digitonin (plasma membrane permeabilized), BeauIII selec

169 subjected to saponification, extraction, and digitonin precipitation.

170 Treatment of mitochondria with digitonin resulted in a 2-fold higher release of cytochr

171 solubilization of rough microsomes (RM) with digitonin, ribosomes co-sedimented in complexes containi

172 Digitonin solubilization of mitochondria demonstrated th

173 Digitonin solubilization of thylakoid membranes releases

174 recipitated by immobilized streptavidin from digitonin-solubilized cells that had been treated with N

175 ontaining the RC-LH1 and -LH2 complexes from digitonin-solubilized chromatophores revealed high level

176 Pull-downs of HA-tagged Cho1 from the digitonin-solubilized fraction reveal an apparent MW of

177 Separation of digitonin-solubilized mitochondrial extracts in one- and

178 biotinylated-Coq3 and Coq4 polypeptide from digitonin-solubilized mitochondrial extracts shows their

179 The digitonin-solubilized OT complex was catalytically activ

180 hown that Cu(2+)-phenanthroline treatment of digitonin-solubilized preparation provides the most effi

181 aled that PDH binds to CL, and supplementing digitonin-solubilized TAZ-KO mitochondria with CL restor

182 electrophoresis and coimmunoprecipitation of digitonin-solubilized thylakoids showed that Hcf106 and

183 crylamide gel electrophoresis (BN-PAGE) from digitonin-solubilized thylakoids were similar in the wil

184 osome does not define the composition of the digitonin-soluble translocon.

185 Whereas in digitonin solution a highly electrostatic ribosome-trans

186 otein-coupled receptor (RGR) was isolated in digitonin solution from bovine RPE microsomes and copuri

187 Association produces digitonin stable complexes with an estimated mass of 950

188 g-8 with Glu and of gGlu-83 with Lys rescued digitonin-stable F-ATP synthase dimers.

189 tochondria with increasing concentrations of digitonin suggested that the NADH dehydrogenase is loose

190 f cellular beta1A integrin is extractable in digitonin, the majority of the transfected beta1D is dig

191 different techniques: permeabilization with digitonin to allow extracellular ATP to equilibrate with

192 ation of the eukaryotic plasma membrane with digitonin to measure Yop targeting during Yersinia infec

193 yed in situ using very low concentrations of digitonin to render their plasma membrane permeable to s

194 Using a low concentration of digitonin to selectively permeabilize the plasma membran

195 use of the ability of low concentrations of digitonin to selectively permeabilize the plasma membran

196 croscopy of cells treated with the detergent digitonin to selectively permeabilize the plasma membran

197 Selective permeabilization of cells with digitonin (to permeabilize the plasma membrane) or Trito

198 In digitonin-treated cells, the electropherograms consisted

199 ration 1, 2, and 5 days posthepatectomy when digitonin-treated microsomes were used.

200 es, p-nitrophenol and estrone, in intact and digitonin-treated microsomes.

201 re, the striking observation that controlled digitonin treatment caused a massive and very rapid rele

202 d to this meshwork, being extracted by brief digitonin treatment.

203 and timed exposure to the pore-forming agent digitonin, we controlled the plasma membrane permeation

204 basal respiration was slightly increased by digitonin when the cells were incubated in medium contai