ライフサイエンスコーパス: divalent ion

1 nnel of the trimeric Dut where it chelates a divalent ion.

2 here with little preference for a particular divalent ion.

3 ne hydroxyl of proteins in the presence of a divalent ion.

4 pressed with increasing concentration of the divalent ion.

5 als that the binding affinity is enhanced by divalent ion.

6 ut a single monomer binds in the presence of divalent ion.

7 cules in a mixture containing monovalent and divalent ions.

8 63, and D65, all have decreased affinity for divalent ions.

9 mersed in a mixed solution of monovalent and divalent ions.

10 er ions selectively when compared with other divalent ions.

11 ch contain not only monovalent ions but also divalent ions.

12 regions through an "induced coalescence" of divalent ions.

13 the same whether supported by monovalent or divalent ions.

14 t cations, chloride, and to a lesser extent, divalent ions.

15 y complex but less so for the monovalent and divalent ions.

16 n their relative stability in monovalent and divalent ions.

17 tion in the nominal absence of extracellular divalent ions.

18 as a neutral pH optimum and does not require divalent ions.

19 unction of polyion charge in the presence of divalent ions.

20 ndent channel binding functions for external divalent ions.

21 tegrity and increased sensitivity to monoand divalent ions.

22 n the presence of 1.6 M NH4Cl and absence of divalent ions.

23 e C and N-terminal sites can also bind other divalent ions.

24 this transition was found to be enhanced by divalent ions.

25 ither activating (Pb2+) or inhibitory (Mg2+) divalent ions.

26 o mica and imaged in situ in the presence of divalent ions.

27 monovalent cations at low levels of external divalent ions.

28 ovided with KCl (< 100 mM) than tested other divalent ions.

29 residues, and its activity is stimulated by divalent ions.

30 ree of esterification and in the presence of divalent ions.

31 may help to understand what makes an MR pump divalent ions.

32 o monitor DNA structure fluctuations without divalent ions.

33 to GO functional group bridging with NOM and divalent ions.

34 the presence of Mg(2+) and in the absence of divalent ions.

35 folds into its tertiary structure with bound divalent ions.

36 ferentially stabilized by Mg(2+) and similar divalent ions.

37 e adsorption was reported in the presence of divalent ions.

38 hich we use to quantify the cross-linking by divalent ions.

39 terminal tail domain mediate crosslinking by divalent ions.

40 of defined concentrations of monovalent and divalent ions.

41 nserved nucleotides, acting independently of divalent ions.

42 ions, but large deviations are observed for divalent ions.

43 protein, both in the presence and absence of divalent ions.

44 hrough an anionic transition state, and that divalent ion activation is substrate dependent.

45 Surprisingly, although divalent ion activation of Nsp15 is widely considered to

46 This study compares alpha and beta divalent ion affinities in Na(+) and K(+) solutions.

47 ng calorimetry and chemical denaturation and divalent ion affinity by titration calorimetry.

48 ated within the E helix, strongly influences divalent ion affinity in the mammalian beta-PV isoform a

49 nts are conducted in K(+)-containing buffer, divalent ion affinity is markedly higher.

50 Interestingly, the divalent ion affinity of Phl p 7 is unexceptional.

51 e AB domain do not necessarily influence the divalent ion affinity of the CD-EF domain.

52 herefore, the impact of the L85F mutation on divalent ion affinity was examined in rat beta-PV, in th

53 These data suggest that parvalbumin divalent ion affinity, particularly that of rat alpha, c

54 lthough all four proteins display heightened divalent ion affinity, the increases are small.

55 whether Phl p 7 likewise exhibits anomalous divalent ion affinity, we have also characterized Bra n

56 placement of Ser-55 with aspartate heightens divalent ion affinity.

57 at alpha-parvalbumin substantially increases divalent ion affinity.

58 elix, which may contribute to the heightened divalent ion affinity.

59 s-suggesting that the AB domain can modulate divalent ion affinity.

60 However, in the absence of divalent ion, alterations at position 257 increase the e

61 complete absence of intra- and extracellular divalent ions, although shifted to higher osmolarities (

62 binding to simple dsRNAs is not regulated by divalent ion, analysis of the interaction of the isolate

63 ded cuts and were independent of pH, type of divalent ion and chromatin repeat length.

64 close vicinity can efficiently coordinate a divalent ion and hold the peptide in a favorable configu

65 In the presence of divalent ions and at low temperature, previous electron

66 ocytes were also sensitive to block by these divalent ions and by DIDS but the sensitivity of ClC-2 t

67 VrtC can function in the absence of divalent ions and can utilize similar naphthacenedione s

68 ndent docking versus approximately 1 for the divalent ions and Co(NH(3))(6)(3+).

69 e of a mutant in the presence and absence of divalent ions and compare it with previous divalent ion-

70 roup I ribozyme in the presence of mono- and divalent ions and PEG crowders of different molecular we

71 -charge density and the interactions between divalent ions and surface groups.

72 514) and Arg(517)-all coupled to active site divalent ions and the DNA motion.

73 This protease activity is regulated by divalent ions and thiol-disulfide conversion in vitro.

74 esting state of VP1 dimer is stabilized by a divalent ion, and chelation using EDTA increases capsid

75 observed depending on nucleotide substrate, divalent ion, and pH.

76 duced by separation of the C-terminal tails, divalent ions, and reducing agents.

77 nding/unbinding in high monovalent salt with divalent ions, and to further interpret noted chromatin

78 d for GTP hydrolysis by the Rho GTPases, the divalent ion apparently participates in the GTPase react

79 Our data confirm that divalent ions are dispensable for catalysis and show tha

80 In reactions with 5 mm Mg2+, other free divalent ions are not needed.

81 vivo, colocalization of actin filaments and divalent ions are suppressed, and cells rely on linker p

82 er and distribution of excess monovalent and divalent ions around a short RNA duplex.

83 letal CRC in experiments using both mono and divalent ions as current carriers.

84 er selenide nanocrystals using two different divalent ions as guest cations (Zn(2+) and Cd(2+)) and c

85 ce of protein structure, lipid demixing, and divalent ions, as well as the physiological implications

86 r biophysical experimentation suggested that divalent ions associate with the M-box RNA to promote a

87 lts may be extended to the emerging field of divalent ion-associated amyloidosis.

88 ence between reaction constants of different divalent ions at the ideal condition explains why not al

89 opposite ways, we postulate that binding of divalent ions at the two sites interact.

90 For divalent ions, at very high ion concentration, further a

91 smolarity, release events following entry of divalent ions (Ba2+ or Ca2+) were less frequent.

92 Formulating ASOs with divalent ions before injection and avoiding phosphate-ba

93 is suggested that a region of "intermediate" divalent ion binding affinity, in between highly ligated

94 differences beyond the binding site modulate divalent ion binding behavior.

95 have explored the linkage of monovalent and divalent ion binding in the folding of the P4-P6 domain

96 ant, the kappa-zeta element is shown to be a divalent ion binding pocket, indicating that this region

97 We have also examined the divalent ion binding properties of the alpha 94/98E vari

98 Using a gating mutant at the divalent ion binding site, we were able to characterize

99 the heavy and light chain of alpha(IIb), the divalent ion binding sites on alpha(IIb), and at a disul

100 from PB theory in competitive monovalent and divalent ion binding to a DNA duplex.

101 The effect of divalent ion binding to deionized bacteriorhodopsin (dI-

102 ely charged residues in the loop regions for divalent ion binding.

103 ion-binding motif contribute to the unusual divalent ion-binding behavior associated with the rat be

104 elds a K(Na) of 630 M(-1) and indicates that divalent ion-binding is positively cooperative.

105 f divalent ions and compare it with previous divalent ion-bound TmCorA structures.

106 low Km for ATP, (b) sensitivity to external divalent ions, (c) lack of desensitization/inactivation,

107 The presence of divalent ion Ca(2+) significantly hydrophobized biofilm,

108 he RNase H complexes formed with one or both divalent ions can be individually observed and character

109 Conversely, interactions with divalent ions can be used to tether headgroups in-plane,

110 ces in the system or a smaller radius of the divalent ions can cause a more abrupt compaction transit

111 interactions in AL-DNA systems are complex: divalent ions can mediate strong attractions between dif

112 under three states reveals that ligands and divalent ions can stabilize similar RNA global conformat

113 receptor potential melastatin 7 (TRPM7) is a divalent ion channel with a C-terminally located alpha-k

114 ikely to apply to other structurally similar divalent ion channels.

115 converges in the two buffer systems, as the divalent ion concentration approaches approximately 1 mM

116 urrent results suggest that changes in local divalent ion concentration in the ribosome could profoun

117 d solution properties, such as pH, salt, and divalent ion concentration on the turnover number of the

118 e fraction effect (AMFE) only when the total divalent ion concentration was 5 mM, consistent with a m

119 The divalent ion concentration was set at 100 mM, to compare

120 erplay between native tertiary interactions, divalent ion concentration, and non-native secondary str

121 vage was measured as a function of mono- and divalent ion concentration, temperature, and pH.

122 ions at the interface grows with increasing divalent ion concentration.

123 onformation as a function of either mono- or divalent ion concentration.

124 Changes in monovalent and divalent ion concentrations drive an abrupt switch betwe

125 lly active on its own at high monovalent and divalent ion concentrations, four protein subunits are a

126 ndirectly on the hFOB cells by reducing free divalent ion concentrations, whereas pamidronate and zol

127 eriments over a wide range of monovalent and divalent ion concentrations.

128 ing preference is amplified at physiological divalent ion concentrations.

129 g experiments over a range of monovalent and divalent ion concentrations.

130 d when folding is initiated by monovalent or divalent ions, consistent with equilibrium measurements

131 in and critically depends on the presence of divalent ions, consistent with results from small-angle

132 Divalent ions counteract this decondensation effect by m

133 tyrosine-containing substrate and Mn as the divalent ion defined a ternary complex mechanism with AD

134 proach would be similar to the inhibition of divalent ion dependent strand transfer by HIV integrase

135 MC-to-Ca(V)alpha(2)delta hand-off involves a divalent ion-dependent step and Ca(V)1.2 element orderin

136 induced in minimal essential media and under divalent ion-depleting conditions; it also participates

137 By comparison, free levels of the divalent ion do not influence maximum turnover (kcat) an

138 , and ESI-MS titrations confirmed that these divalent ions do not appreciably bind to P7.

139 w that lactate, acting through extracellular divalent ions, dramatically increases activity of an aci

140 o slow down the structure fluctuations using divalent ions (e.g., Mg(2+)).

141 To examine divalent ion effects over a wide concentration range, ur

142 In this current study, ionic strength and divalent ion effects were probed.

143 Decreasing the size of the divalent ions enhances the electrostatic bending attract

144 However, RED using feed streams containing divalent ions experiences lower power densities because

145 ns; and single-channel conductance to Na+ in divalent-ion-free conditions.

146 Divalent ions fulfill essential cellular roles and are r

147 Divalent ions further compact the fiber by promoting ben

148 crotiter wells in the presence of fetuin and divalent ions in a carbohydrate-dependent manner.

149 s universal reliance, the functional role of divalent ions in promoting RNA catalysis is manifold.

150 ge of the bilayer as well as the presence of divalent ions in the buffer play an important role.

151 the presence of carboxyl groups on ENPs and divalent ions in the solution plays a key role in contro

152 d conductance was inhibited by extracellular divalent ions including Ba(2+) (K(i) = 0.7 mM) and Ca(2+

153 We found that extracellular divalent ions, including Ca(2+), inhibit the permeation

154 However, it also binds other divalent ions, including Cd(2+), Cu(2+), Mn(2+), and Mg(

155 the HDV ribozyme can self-cleave by multiple divalent ion-independent and -dependent channels, and in

156 on ion-binding distribution reveals that the divalent ion-induced helix bending attraction may come f

157 ofibers are described, including pH control, divalent ion induction, and concentration.

158 In contrast to more acidic Me2+ divalent ion inhibitors of the high-affinity Mn2+ site,

159 ent carried by Na+ and Cs+ in the absence of divalent ions (Ins) also activated at more negative pote

160 nism, separate from fast adaptation, whereby divalent ions interacting with the local lipid environme

161 ripping voltammetric current response to the divalent ion is enhanced to achieve a subnanomolar detec

162 ke and release, but in this case the primary divalent ion is Zn(2+) rather than Ca(2).

163 Thus, the reduced affinity for divalent ions is evidently not the result of heightened

164 dsorption to negatively charged surfaces via divalent ions is extensively used in the study of biolog

165 The inclusion of divalent ions leads to a reversal of the binding affinit

166 RNA domain, with one monovalent and several divalent ions located in specific sites within the struc

167 anism for vimentin networks and suggest that divalent ions may help regulate the cytoskeletal structu

168 a 10-fold activity change of monovalent and divalent ions measured at room temperature, respectively

169 Divalent ions Mg(+2), Zn(+2), Co(+2), Hg(+2) and Cd(+2)

170 lent ions (K(+), Na(+) and Li(+)) as well as divalent ions (Mg(2+) and Ca(2+)), outperforming existin

171 monovalent ions (Na(+) and K(+)) compared to divalent ions (Mg(2+) and Ca(2+)).

172 ity of Gfh proteins depends on the nature of divalent ions (Mg(2+) or Mn(2+)) present in the reaction

173 teins 8 (K8) and 18 (K18) in the presence of divalent ions (Mg(2+)).

174 Divalent ions (Mg, Ca, and Zn) are being considered as c

175 ther globally or locally, in the presence of divalent ions, might constitute a mechanism for regulati

176 erence between AS-1 and AS-2.2) Although the divalent ion Mn(2+) enhances the ligand binding function

177 In the absence of divalent ions, NCS-1 unfolds and refolds reversibly in a

178 tructure, which retains many common sites of divalent ion occupation.

179 esolves the differential impact of mono- and divalent ions on chromatin conformations.

180 ed by determining the effects of seven other divalent ions on erythrocyte membrane properties.

181 or optimal activity; however, the effects of divalent ions on Nsp15 reaction kinetics have not been i

182 , we investigated the influence of different divalent ions on the activity of HtrA.

183 y of reversible binding effects of mono- and divalent ions on the chemical shift properties of the Le

184 tify the influence of diffuse monovalent and divalent ions on the dynamics of biomolecular assemblies

185 re generated by exposing alpha4-integrins to divalent ions or by inside-out activation using a chemok

186 ndent inward rectification in the absence of divalent ions or charged regulators such as spermine, in

187 ein-free phase separation in the presence of divalent ions or crowding agents.

188 he general acid does not appear to depend on divalent ions or the identity of the Bronsted base.

189 The preferred storage of divalent ions over their competing monovalent counterpar

190 The presence of divalent ions, particularly calcium, appears to be an im

191 tization of alpha7 and dramatically reducing divalent ion permeability relative to wild-type alpha7.

192 n this study, we demonstrate that calcium, a divalent ion, preferentially increases the activity of c

193 thin the PA channel pore and that H+ and the divalent ions probably act via similar mechanisms to aff

194 We find that divalent ions produce a fiber stiffening effect that com

195 s and find it to be lower in the presence of divalent ions rather than only monovalent ions.

196 ments illustrating the enhanced screening of divalent ions relative to monovalent ions at the same io

197 The concentration and specificity of divalent ions required to induce the fluorescence change

198 alpha 8 beta 1-AP chimera exhibited the same divalent ion requirements for activation and binding spe

199 telomerase was characterized with respect to divalent ion requirements, effect of salt and nonionic d

200 These sequence-dependent, divalent ion-sensitive, and structurally unique solution

201 ion with the lower affinity binding to the A divalent ion site.

202 performed to probe the nature of the various divalent ion sites and any specificity for Mg2+.

203 The pseudoknot binds divalent ions somewhat more tightly than a hairpin but s

204 dence of competitive binding between the two divalent ion species.

205 However, it is also likely that a subset of divalent ions specifically occupies cation binding sites

206 uctances for Ca(2+) than for Na(+) and other divalent ions (Sr(2+) and Mg(2+)) that are eliminated af

207 Mono- and divalent ions stabilize the native fold of RNA, whereas

208 Group II divalent ions stabilized the parallel G-tetraplexes, and

209 The role of cations, particularly divalent ions such as Ca(2+), in promoting eDNA adsorpti

210 Surprisingly, divalent ions such as Mg(2+), Ca(2+), and Zn(2+) act as

211 The specificity of this effect for different divalent ions suggests binding sites that are not an EF-

212 No other divalent ions tested could replace calcium.

213 In eag, these transitions are modulated by a divalent ion that binds in the gating pocket.

214 DNA and correct incoming nucleotide, and two divalent ions, the thumb subdomain of pol X undergoes a

215 In the absence of divalent ions, the tRNA core gains flexibility under con

216 atalyze the reaction in the absence of other divalent ions, they significantly modulate the reaction

217 mimicks the reactions of the highly reducing divalent ions Tm(II), Dy(II), and Nd(II), has been explo

218 )(py)(2), and (dpm)Co(II)(py)(2) reveal each divalent ion to be high-spin and pseudotetrahedral in th

219 Binding of the catalytic divalent ion to the ternary DNA polymerase beta/gapped D

220 Necessity of the divalent ions to retain the suspension signified the ele

221 Assuming specific binding of divalent ions to RNA, the Mg2+ dependence of the observe

222 Our data also show that, during unfolding, divalent ions together with LHs induce linker-DNA bendin

223 between membrane potential fluctuations and divalent ion transport.

224 Solute carrier 11A1 (SLC11A1) is a divalent ion transporter formerly known as the natural r

225 en tested using mutagenesis and two cellular divalent ion uptake assays.

226 carried out a series of experiments to test divalent ion usage and preferences.

227 Furthermore, divalent ion variations continuously tune the microenvir

228 e resultant peak potentials of the secondary divalent ion vary with its sample activity to yield an a

229 lular Ca(2+) , suggesting that influx of the divalent ion via more Ca(2+) -permeable normal MT channe

230 to block of outward current by intracellular divalent ions, we find that inward rectification is in f

231 significant level over a control in which no divalent ions were added to the media.

232 emerge from the binding energies of the six divalent ions with amino acids and dipeptides.

233 Delocalized electrostatic interactions of divalent ions with nucleic acids should be very weak in

234 endence emphasizes the strong interaction of divalent ions with the membrane and its effect on the me

235 IK(IR) is also blocked by other divalent ions, with Ba2+ >> Sr2+ > Mg2+ > Mn2+ = Ca2+, a

236 selectivity to the dehydration of the large divalent ions within the subnanometer pores.