ライフサイエンスコーパス: double mutant

1 ating phase of seed development in the e2fab double mutant.

2 nation defects observed in the mpk4RNAi/mpk3 double mutant.

3 hickening promoting factor1-1 (nst1-1)nst3-3 double mutant.

4 rginine deiminase genes were observed in the double mutant.

5 ion were partially restored in an rsmB, rsmS double mutant.

6 rotein extract prepared from the pir1-1/pir2 double mutant.

7 ible than WT plants, as was the gh3.7/gh3.12 double mutant.

8 ures that is more extreme than the uch1 uch2 double mutant.

9 selected for revertants of the E2-H348/352A double mutant.

10 0 value of 1 nM against the EGFR L858R/T790M double mutant.

11 olonged longevity, which was enhanced in the double mutant.

12 these effects are exacerbated in a mneP mneS double mutant.

13 utant and was also increased in an opaR aphA double mutant.

14 egraded, in neurons infected with a gE-/US9- double mutant.

15 ial Q(6) content in the coq10Deltacoq11Delta double mutant.

16 hen EXECUTER1 (EX1) is absent in the flu ex1 double mutant.

17 ion whereas spores were nearly absent in the double mutant.

18 tant but partially restored in an rsmB, rsmS double mutant.

19 and the phenotypes of cry1a/cry2 single and double mutants.

20 nes in the CD model that are not seen in the double mutants.

21 n ipk1Delta kcs1Delta or ddp1Delta kcs1Delta double mutants.

22 aCRY and created the chr1 chr2 and uvr8 phot double mutants.

23 in genes, generating all possible single and double mutants.

24 mes in the sensory axons of spectrin and tau double mutants.

25 Ps, and BPs were entirely lost in Jag1; Dll1 double mutants.

26 reiterated defects observed in adult Bmp9/10 double mutants.

27 2/3/4/5 quintuple, yda single, and mkk4 mkk5 double mutants.

28 ssion and cell death, increase in older crwn double mutants.

29 Mutation of UIEF1, UIEF2, or both in the double-mutant 2xuief caused modest growth defects.

30 Double-mutant 5xF:pGB mice displayed improvements in cog

31 In addition, mice expressing double-mutant A20 proteins in A20's ZF4 and ZF7 motifs d

32 Roots of the double mutant aar1-1 tir1-1 also showed enhanced resista

33 Here we show that gE(-) US9(-) double mutants accumulate large quantities of unenvelope

34 m, DeltaBbSre1 or the DeltaBbOhmmDeltaBbSre1 double mutant accumulated high levels of heme and mitoch

35 on, and when coexpressed with KIN10 the WRI1 double mutant accumulated to 2- to 3-fold higher levels

36 ramatic synergistic effect was found for the double mutant across all parameters.

37 The DeltainvDeltayadA double mutant adhered least to cells and so was not sign

38 In yeast, the pol3-01,L612M double mutant allele, which causes defects in DNA polyme

39 The double mutant also failed to proliferate in medium suppl

40 s are required for survival of both types of double-mutant AML.

41 Double-mutant analyses support a specific functional int

42 Together with transcriptomic and double-mutant analyses, our data revealed that the HUB1

43 on (i.e., over hundreds of micrometers), and double mutant analysis supports that FP-netrin1 and Shh

44 e sensitivity of germination to low N in the double mutant and differential expression of nitrate tra

45 iptomic analyses on Arabidopsis gpa1-5gcr1-5 double mutant and identified 656 differentially expresse

46 s impaired the infectivity of both the F-MLV double mutant and the wild-type F-MLV strain, suggesting

47 restored to wild-type levels in a luxO opaR double mutant and was also increased in an opaR aphA dou

48 phloem formation are impaired in smxl4;smxl5 double mutants and that the additional cambium-derived c

49 also significantly enhanced in the nos srrAB double mutant, and its aerobic growth defect could be pa

50 tion was restored in the DeltaflbA DeltamcrA double mutant, and overexpression of mcrA completely blo

51 dopsis, we compared phenotypes of single and double mutants, and analyzed the effect of ABA4 overexpr

52 n transport, as shown by transport-deficient double mutants, and depended on Cl(-)/H(+) exchange, as

53 d the structure of vegetative SAMs in ltm sp double mutants, and late flowering was partially suppres

54 n complement the severe phenotype of brx ops double mutants, and the most active variants eventually

55 In these Apc-Grp78 double mutant animals, stem cells were rapidly lost and

56 ding of amyloid-beta pathology in transgenic double-mutant APPSwePSEN1dE9 mice.

57 regulation of nodulation and Ljein2a Ljein2b double mutants are hypernodulating and hyperinfected.

58 atal movements of agb1 mutants and agb1/gpa1 double-mutants, as well as those of the agg1agg2 Ggamma

59 The double mutant badc1 badc2 phenocopied wri1-1 with respec

60 isplay altered ABA response, the pir1-1/pir2 double mutant became more insensitive to ABA than the wi

61 gan failure in kl/kl mice was rescued in the double-mutant BK/BK;kl/kl mice exhibiting lower plasma P

62 Thyrocyte-specific double-mutant BRAF(V600E) PIK3CA(H1047R) mice were treat

63 A wdr92-1 tpg1-2 double mutant builds ~7-mum immotile flaccid cilia that

64 The C201S, C296S double-mutant C/EBPbeta prevented protein S-glutathionyl

65 lting free energies associated with pairwise double mutants can be predicted with quantitative accura

66 Interestingly, one of our predicted double mutants catalyzes dehalogenation of 1,2-dibromoet

67 Double mutant cells are able to grow under excess illumi

68 Yet the double mutant cells grow, recruit MCM2-7 normally to chr

69 ion and IDH2(R140Q), with only recipients of double mutant cells succumbing to leukemia.

70 IDH2 and SRSF2 double-mutant cells exhibited aberrant splicing and redu

71 However, double-mutant cells fail to complete HR, as excessive sh

72 ong-term tracking of Tet2 mutant or Tet2/Id3 double-mutant cells in our MASCOT model revealed a dynam

73 a channel recordings, the conductance of the double-mutant channel was unaffected by extracellular ac

74 ong and broad loss of neural APA in elav/fne double mutant CNS, the first genetic background to large

75 total EMB genes in Arabidopsis; document 83 double mutant combinations reported to disrupt embryo de

76 Characterization of all double mutant combinations revealed that although the mt

77 although the mtacp1 mtacp3 and mtacp2 mtacp3 double mutant combinations showed no observable growth d

78 Different double mutant combinations showed that a low amount of C

79 ingle mutant, and phenotypic observations in double mutants combining bk4 with bk2 or null alleles fo

80 Finally, the double mutant, combining both mutations, showed a synerg

81 arget genes was strongly reduced in eol1 clf double mutants compared with clf single mutants.

82 ptotic cell density in Foxg1(-/-);Wnt8b(-/-) double mutants compared with the Foxg1(-/-) single mutan

83 he wild type, while those of the ptst2 ptst3 double mutant contained even fewer granules than ptst2 T

84 find that myp2Delta and myp2Delta myo51Delta double mutants contract actomyosin rings at approximatel

85 Growth defects of the double mutants could be eliminated by overexpressing PNP

86 In contrast, a spe2Delta alr1Delta double mutant cultured in SDC exhibited little increase

87 Double mutant cycle analysis revealed a strong relations

88 eltaDeltaGo values >4.2 kJ/mol obtained from double mutant cycle analysis.

89 Double-mutant cycle analyses revealed that C(10) substit

90 lar dynamics simulations as well as chemical double-mutant cycle analysis.

91 -vis titrations in combination with chemical double-mutant cycles (DMCs) have been used to study the

92 vir-terminated primers, as compared with the double-mutant D67N/K70R.

93 99 with T43I and observed that the T43I/A21G double mutant decreases Abeta40 formation.

94 Infection with double-mutant DeltaM36/M45mutRHIM virus reveals a signal

95 7A1 failed to recognize two Der p 2 epitope double mutants designed to abolish the allergen-Ab inter

96 velopment in accessions, and cyp79b2 cyp79b3 double mutants developed fewer and shorter lateral roots

97 Arabidopsis double mutants devoid of functional PRX9 and PRX40 are m

98 ll for pRb, p107, p130 or any combination of double mutants did not develop melanoma.

99 D1 prod double mutants die during embryogenesis, exhibiting enha

100 g USP11-WT versus a binding pocket-deficient double mutant disclosed that this binding site modulates

101 A insertion mutant erf74 and the erf74;erf75 double mutant displayed higher sensitivity.

102 ortholog mbl-1 and the ELAVL ortholog exc-7, double mutants displayed a severely shortened lifespan.

103 Many double mutants displayed fitness defects, revealing synt

104 Syt1/Syt7 double mutants displayed more release than Syt1 mutants

105 The double mutant (DM) L546A/L754A is considered a dramatic

106 However, we show that the BM3 A82F/F87V double mutant (DM) variant binds substantially tighter t

107 One of these cultivars was a brown-midrib double mutant (DM) which had reduced levels of lignin co

108 e of markedly low tissue levels of iron, the double mutant does not up- and down-regulate iron defici

109 We show that the DRM methyltransferase double mutant drm1drm2 also presents ectopic enlarged ce

110 C60U) that is efficiently trapped by a TrmA* double mutant (E49R;R51E) but not by the wild-type TrmA*

111 3 and WRINKLED 1 is upregulated in the e2fab double mutant embryo.

112 lial adhesions in the most severely affected double mutant embryos ( Irf6(+/-);Tg(KRT14::Spry4)).

113 masses present in the epaxial region of the double mutant embryos and are able to divide and contrib

114 At E14.5, the number of Thm1;Thm2 double mutant embryos is lower than that for a Mendelian

115 The pmt1 pmt2 double mutant enhanced the defects in root growth, cell

116 bpm lines, bpm235 triple mutants, and cul3ab double mutants enhances MYC2 and MYC3 stability and accu

117 rystallography, we show that the P167S/D240G double mutant enzyme exhibits decreased ceftazidime hydr

118 Using a genetics approach with the double mutants era1 abi1-1 and era1 ost1, we show that w

119 Results from tmc single and double mutants evince a principle role for Tmc2a and Tmc

120 The fact that wss1A mus81 and tdp1 mus81 double mutants exhibit growth defects and an increase in

121 llg2 llg3 double mutants exhibit severe fertility defects.

122 The CaS(S875A/T888A) double mutant exhibited even greater Ca(2+) (o) sensitiv

123 Double mutants exhibited a more severe phenotype, with i

124 ormal rhythmicity, whereas Dh31(#51);Pdf(01) double mutants exhibited a severe arrhythmic phenotype c

125 Surprisingly, the gpa-3;nlp-24 double mutants exhibited much higher dauer formation tha

126 The double mutant exhibits a delay in cohesin removal during

127 A DeltarecGDeltaradD double mutant exhibits an almost complete growth defect,

128 However, the osvoz1 osvoz2 double mutant exhibits strong dwarfism and cell death, a

129 but chorions from eggs laid by larp6a;larp6b double mutant females were more defective than those fro

130 he grin1 knockout is embryonic lethal, grin1 double-mutant fish (grin1a (-/-) ; grin1b (-/-)), which

131 number was not affected either in single or double mutants for the activator-type E2FA and E2FB Acco

132 d in conjunction, generating both single and double mutants for the putative siderophore systems.

133 n of an M. tuberculosis sigE fadD26 unmarked double mutant fulfilling the criteria of the Geneva Cons

134 Phenotypes of the double mutant fvemir164a fvecuc2a indicated that the two

135 developed normal spikelets, but ap2l2 ap2l5 double mutants generated spikelets with multiple empty b

136 gametophytes could transmit the dgat1 plip1 double-mutant genotype.

137 In contrast, the trt1Delta blmDelta double mutant gives rise to survivors as readily as the

138 DeltauroS mutant and the DeltauroS DeltayifB double mutant had a severe survival defect compared to t

139 logy to wild-type granules, but those of the double mutant had an aberrant morphology.

140 ce with combined mutations in Zeb2 and Edn3 (double mutants) had more severe enteric anomalies and in

141 recycling can also be observed in single and double mutants harboring vps35Delta.

142 ice1 or zou increases seed dormancy and the double mutant has an additive phenotype.

143 The fnrS arcZ double mutant has phenotypes in a mouse oral infection m

144 We show that tan1 air9 double mutants have a synthetic phenotype consisting of

145 The tan1 air9 double mutants have significant defects in division plan

146 mug) administered with the Escherichia coli double mutant heat-labile toxin (dmLT) adjuvant afforded

147 e toxin B subunit (LTB) chimera admixed with double mutant heat-labile toxin (LT) LT-R192G/L211A (dmL

148 oxoid (LCTBA) administered with or without a double-mutant heat-labile enterotoxin (dmLT) as an adjuv

149 The smyd1a and smyd1b double mutants, however, showed a stronger muscle defect

150 To conclude, we developed long-living double mutant hSOD1/rag2 mice, which could be a promisin

151 solic loop of NCKX4 and was abolished by the double-mutant I328D/F334D.

152 substrate specificity are not rescued in the double mutant, implying that functional sequence variati

153 ISPR mutagenesis, we obtained an nrg1a-nrg1b double mutant in two Arabidopsis accessions, and an nrg1

154 f flowers to leafy shoots, mimicking lfy ap1 double mutants in A. thaliana.

155 WT, med12, aux1-7 and med12 aux1 single and double mutants in response to sucrose and/or N-1-naphthy

156 in decreased proteolytic stability of the Rz double mutants in vivo Unlike the wild type, in which ly

157 scle defects in smyd1a and smyd1b single and double mutants in zebrafish.

158 es of the monolignol pathway, as well as one double mutant, in the model legume Medicago truncatula T

159 This was revealed by genetic studies with double mutants including carotenoid isomerase (yofi), a

160 The adg1suc2 double mutant increases glucose plus sucrose content in

161 idopsis (Arabidopsis thaliana) ctl1-1 ctl2-1 double mutants indicated that Ctl1 might have a conserve

162 s substantially reduced in atpat21-1 spo11-1 double mutants, indicating that AtPAT21 is required for

163 g synergistic effect with the PR-D212N,F234S double mutant, inducing an astonishing 200 nm red shift

164 mployed in previous studies, a RanT24N, T42A double mutant inhibits RanGEF without perturbing cargo b

165 notypes of acs or acn1 mutants, the acs acn1 double mutant is delayed in growth and sterile, which is

166 tive diversity follows, caused by highly fit double-mutant 'jackpot' clones that are fed from exponen

167 tively active K-Ras4B as our control and two double mutants (K101D and R102E; and R41E and K42D) in t

168 lso seems to be involved, since dpe2-1/phs1a double mutants lacking both PHS1 and the cytosolic DISPR

169 Phenotypic analysis of a suite of double mutants lacking both SS5 and other proteins impli

170 Double mutants lacking receptor tyrosine phosphatases (P

171 Hence, we could select double mutants lacking the T-DNA already in the first of

172 Many behavioral defects in the grin1 double-mutant larvae, including abnormal evoked response

173 the sensor in a conditional loss-of-function double-mutant line for the Arabidopsis MAPK genes MPK3 a

174 Importantly, double-mutant Lmna(G609G/G609G)Mmp13(-/-) mice or Lmna(G

175 transfer DNA insertion mutant lpeat2 and the double mutant lpeat1 lpeat2 showed impaired growth, smal

176 While castration of dystrophin and utrophin double mutant (mdx-dm) mice to mimic pre-pubertal nadir

177 pathology in the severe dystrophin/utrophin double mutant (mdx:utr (-/-)) mouse model of DMD.

178 ar cancer stem cell population in Pten/Trp53 double mutant medulloblastomas.

179 Further, Thm1;Thm2 double-mutant MEF show enhanced cilia disassembly, and i

180 The obtained hSOD1/rag2 double mutant mice have been characterized.

181 Although in Apc-Grp78 double mutant mice the Wnt signature was lost, these int

182 mphatic vascular defects seen in Fgfr1/Fgfr3 double mutant mice, while HK2 overexpression partly resc

183 The resultant double-mutant mice 5xF:pGB mice displayed a full rescue

184 The Abcc6(-/-)Alpl(+/-) double-mutant mice showed 52% reduction of mineralizatio

185 We found that Six1 (-/-) Six2 (-/-) double-mutant mice were born with severe craniofacial de

186 In this study, we generated Camk2a/Camk2b double-mutant mice, and observed that loss of CAMK2, as

187 electro-olfactogram (EOG) recordings on the double-mutant mice, NCKX4(-/-);CNGB1(DeltaCaM), which ar

188 Both double-mutant models developed high-penetrance AML, alth

189 elf elevation-80% of Pax9(del/del);Wise(-/-) double-mutant mouse embryos exhibit rescued palatal shel

190 Of note, the double mutant msn2Deltamsn4Delta exhibited a severe grow

191 Here, we used PA as a double mutant (N682A, D683A; mPA) which cannot bind to t

192 ultured SJ1(RQ)KI neurons was more severe in double-mutant neurons.

193 An S144A-S146A double mutant not only bound inappropriately to mitotic

194 Double-mutant NPCs also overexpressed the SIX family mem

195 ur analyses indicate that the defects of the double mutant occur mainly at stage I of lateral root de

196 Recently, a double mutant of Arabidopsis (Arabidopsis thaliana) PHOS

197 ) in a murI (racemase) DeltadapF (epimerase) double mutant of E. coli rescues the d-glutamate auxotro

198 ith STTM165/166, we successfully generated a double mutant of miR160 and miR165/166.

199 sticity of the high-efficiency frameshifting double mutant of the 26 nt potato leaf roll virus RNA ps

200 est this, we generated Foxg1(-/-);Wnt8b(-/-) double mutants of either sex and found that the morpholo

201 transcriptomic analyses using the single and double mutants of gcr1-5 and gpa1-5 identified 194, 139

202 Genetic analyses reveal that phyB1 phyB2 double mutants of maize exhibit abnormal cuticle composi

203 The double mutants of PGK3 and the triose-phosphate transpor

204 e cleavage rates for all possible single and double mutants of this ribozyme across a series of ligan

205 In particular, the double-mutant of paps1 and rna-dependent rna polymerase

206 f expression of BnROD1.A3 and BnROD1.C3 in a double mutant, or by RNA interference, reduced the PUFA

207 essed in tan1 air9 significantly rescued the double mutant phenotype in all three respects.

208 acids, TAN1-DeltaI-YFP, failed to rescue the double mutant phenotype, while TAN1 missing a conserved

209 ion antagonistically affect the alpha Aurora double mutant phenotypes.

210 In the grik1-2 grik2-1 double mutant, phosphorylation of SnRK1.1 was reduced, b

211 Analysis of double mutant plants defective in different combinations

212 on, as SL-deficient and ethylene-insensitive double mutant plants display essentially additive phenot

213 The double mutant plants exhibited a series of compromised p

214 re partially reversed when the mtacp1 mtacp2 double mutant plants were grown in a nonphotorespiratory

215 We found that the ala1/ala2 single- and double-mutant plants exhibited enhanced disease suscepti

216 However, tex1 mos11 double-mutant plants show marked defects in vegetative a

217 In contrast, the double mutant pph1;pbcp appeared strongly locked in stat

218 The ptaA/pvdN double mutant produced exclusively the glutamic acid for

219 ysis of the non-leukemic single and leukemic double mutant progenitors, isolated from these mice, sug

220 aralog, and of trigenic interactions for the double mutant, provides insight into their roles and a q

221 Ttc7(fsn/fsn)) mice were crossed to generate double-mutant (Rag2(-/-)Ttc7(fsn/fsn)) and triple-mutant

222 Analysis of double-mutant rates and associated mutational interactio

223 Mesoderm-specific Fgf4/Fgf8 double-mutants recapitulated anterior mesoderm defects a

224 by the severe attenuation of virulence in a double mutant relative to the single individual mutation

225 ESC with a Wnt inhibitor, but not ESRRB/FLCN double mutant, rescues the cells, allowing the exit from

226 e during mouse infection, while compensatory double mutants restored virulence to WT levels.

227 he individual mtrP and tmaT mutants and of a double mutant revealed strikingly similar changes across

228 alysis of ubr-5 and ubr-7 single mutants and double mutants revealed genetic interactions with the No

229 Examination of tmc double mutants revealed major contributions from Tmc2a a

230 e tail point mutants with those of symmetric double mutants revealed that a single methylated H3K36 p

231 ck-resistant and loss-of-function single and double mutants revealed that each AHAS and IPMS isoenzym

232 e spxA1 strain as background for creation of double mutants revealed that four of the five genes inac

233 Analysis of the lls1 sgl1 and lls1 palm1 double mutants revealed that SGL1 is epistatic to LLS1,

234 FM labeling of lateral cristae in tmc double mutants revealed the presence of two distinct cel

235 s they were expressed in similar tissues and double mutants revealed the wide-angled lazy1 branch phe

236 insensitive to CPT, and only the wss1A tdp1 double mutant reveals a higher sensitivity than the wss1

237 with the synergistic growth phenotype of the double mutant rh50-1 gun1-102, suggest that RH50 and GUN

238 her number of negative interactions than the double mutant SGA screens and uncovered additional genet

239 nrp1-1 nrp2-2 double mutants show an over-accumulation of H2A.Z genome

240 -of-function ubc26 alleles and the rfa1 rfa4 double mutant showed enhanced sensitivity to ABA and acc

241 alysis of a flowering locus t3 (hvft3) hvcen double mutant showed that HvCEN interacts with HvFT3 to

242 eral constructed mutants, Ala96Leu/Asn212Lys double mutant showed the highest dye-mediated dehydrogen

243 sR and lasR pqsR mutants was found, in which double mutants showed less pyocyanin and protease produc

244 The double-mutants showed no gross morphological changes of

245 the pathogen Pseudomonas syringae, with the double mutant showing a stronger phenotype.

246 ugar/energy sensing, and the grik1-1 grik2-1 double mutant shows growth retardation under regular gro

247 ies validate these attributes as Arabidopsis double mutants singularly expressing BADC2, BADC3, or BA

248 r increased Mg(2+) accumulation, as all four double mutants (spe1Delta alr2Delta, spe2Delta alr2Delta

249 ped recombination, suggesting that Apc-Grp78 double mutant stem cells had lost self-renewal capacity.

250 pment, the roots of ein3 and eil1 single and double mutants still respond to ethylene in light-grown

251 he severe arrhythmicity of Dh31(#51);Pdf(01) double mutants, suggesting that DH31 and PDF may act on

252 all three point mutants and >90% for the WRN double mutant (T1024G/T1086G) relative to normal B-form

253 terization of three single mutants and three double mutants targeting the sugar-binding pockets ident

254 ats and after genetic elimination of TGR5 in double mutant TGR5(-/-) ;Pkhd1(del2/del2) mice.

255 4 ABA receptors were higher in the rfa1 rfa4 double mutant than in wild-type plants.

256 s We obtained a L. japonicus Ljein2a Ljein2b double mutant that exhibits complete ethylene insensitiv

257 wild-type reversal frequency are restored in double mutants that are defective in both EPS production

258 Double mutants that combined a stabilizing substitution

259 identify a Chimaerin (CHIN-1)- Furin (KPC-1) double-mutant that severely disrupts assembly.

260 In the S200V/S201V double mutant, the proton affinity of E286 is increased,

261 Nevertheless, in tex1 mos11 double-mutants, the mRNA export defect was clearly enhan

262 We use the tan1 air9 double mutant to discover new functions for TAN1 and AIR

263 We generated an elf3 gi double mutant to study their genetic relationship in clo

264 In addition, we generated a double mutant to test genetic interactions of the CRA2 s

265 WT, med12, aux1-7 and med12 aux1 single and double mutants to sucrose and application of auxin trans

266 We next used single and double mutants to test whether mutating both TFs will mo

267 The double mutant (TxGT) showed reduced growth in air but no

268 the Arabidopsis hypersensitive bzip19 bzip23 double mutant under Zn deficiency.

269 e were unable to generate fkp40(-)/afkp80(-) double mutants, unless one of the A/FKPs was expressed e

270 urther analysis of the DEGs exclusive to the double mutant using protein-protein interaction networks

271 Moreover, an analysis of ahk double mutants using CycB1;1:GUS/ahk introgressed lines

272 ore mRNAs are reduced in a ded1-ts dbp1Delta double mutant versus either single mutant, becoming high

273 macrophages is dramatically increased during double-mutant virus infection and correlates with faster

274 During germination, the camta6 hkt1 double mutant was as sensitive as the wild type and hkt1

275 The pgk1.1 pgk3.2 double mutant was bigger than pgk3.2 and displayed an in

276 A DeltaciaRDeltaargB double mutant was completely restored for the gtfP gene

277 emical levels, whereas the ndufs8.1 ndufs8.2 double mutant was devoid of detectable holo-CI assembly/

278 The G112D/M230I double mutant was less susceptible to NNRTIs than was M2

279 hese cells, the Deltafkp40(-)/Deltaafkp80(-) double mutant was now readily obtained.

280 Growth of a sitA mntH double mutant was severely reduced under Mn(2+) limitati

281 Growth and viability of phr1phl1 double mutant was significantly reduced in phosphate-dep

282 elX In line with this, a DeltapelX DeltapgnE double mutant was substantially impaired in its ability

283 observable growth defect, the mtacp1 mtacp2 double mutant was viable but displayed delayed growth, r

284 e-specific rescue of ga20ox triple and ga3ox double mutants was shown.

285 By comparing cgl160 single with cgl160 kea3 double mutants, we demonstrate that in the cgl160 backgr

286 In connection with the characterized double mutants, we discuss the generation of starch gran

287 utant or an endocytic-defective Y658F-VE-Cad double mutant were both able to rescue TEER independentl

288 and ABA signaling genes in the STTM-directed double mutant were compromised compared to the two singl

289 s4 triple mutant and various combinations of double mutants were generated and metabolically analyzed

290 ishable from that of the WT protein, and the double mutants were secreted to varying extents.

291 Despite this, maternal zygotic single and double mutants were viable and fertile.

292 nts, as well as those of the agg1agg2 Ggamma double-mutant, were insensitive to Ca(o) .

293 hese effects were distinctly pronounced in a double mutant, where the TRXo1 mutation was combined wit

294 e epistasis of exo70A1 in the exo70A1 syp121 double mutant, which shows decreased growth similar to e

295 Further, pair wise comparison of DEGs of double mutant with single mutants of gcr1-5 or gpa1-5 sh

296 Furthermore, early flowering3 (hvelf3) hvcen double mutants with high HvFT1 expression levels under s

297 By making double mutants with the pro-apoptotic genes Bax and Trp5

298 sed in xth9 and reduced in xth4, whereas the double mutant xth4x9 displayed an intermediate number of

299 A ScCBS double mutant (Y158F/Y226V) did not display activity wit

300 terize an Arabidopsis (Arabidopsis thaliana) double mutant, yellow stripe1-like yellow stripe3-like (