ライフサイエンスコーパス: double mutation

1 n in the context of the original lethal NS4B double mutation.

2 nished in plants carrying the cca1-11/lhy-21 double mutation.

3 h conditions is significantly reduced by the double mutation.

4 the deletion was combined with the E12A/D13A double mutation.

5 r the impaired GTP binding of the T192AT193A double mutation.

6 resistance, notably against the G140S-Q148H double mutation.

7 cking of CaValpha2delta was disrupted by the double mutation.

8 a activity was attenuated by the S511A/S515A double mutation.

9 ption factor E2F1, was enhanced by Cry1/Cry2 double mutation.

10 on for the reported nonadditivity effect for double mutations.

11 HP2 by introducing Asp to Ala and Cys to Ser double mutations.

12 d other tumor types, most of which (95%) are double mutations.

13 samples with a wide range of rpoB single and double mutations.

14 ene-positive patients were published to have double mutations.

15 guide clinical decision making in cases with double mutations.

16 n on prevalence and outcome of patients with double mutations.

17 BL/6 mice accumulated significant amounts of double mutations.

18 single NFkappaB mutations and synergistic in double mutations.

19 ; single mutation, 134+/-8 beats per minute; double mutations, 111+/-6 beats per minute; P<0.0001), a

20 fter hepatectomy were worse in patients with double mutation (3-year RFS, 3.1% vs 20% [P < 0.001]; 3-

21 acids (residue 73, 94, 135, 136, or 138 or a double mutation, 93/244) that are not found in circulati

22 Results showed double mutation A1762T/G1764A (TA) combined with other m

23 in activity, while a histidine and tyrosine double mutation abolished cleavage.

24 tion plus MyD88 inhibitor, but not flaA cdtB double mutations, abolished the ability of the supernata

25 mutation and the rpoD3 rpoD4 and rpoD2 rpoD3 double mutations affected expression from the kaiB promo

26 However, a double mutation affecting both serine-219 and the adjace

27 n uptake from both sides of the protein by a double mutation affecting the D pathway and the proton b

28 163E and S177E mutations and the S163E/S177E double mutation all inhibit the ability of PRH to regula

29 The double mutation alpha1(N407A/Y410F) prevents potentiatio

30 112, reduced proton sensitivity, whereas the double mutation alpha1(T112A)beta(T135A) almost complete

31 ubstrate binding and catalysis), or with the double mutations alpha1E525K,C594D, the pseudosymmetric

32 g region in the alpha1 subunit Q242W and the double mutation alpha1N408A/Y411F, previously shown to a

33 The mutation alphaH282D or double mutations alphaH282R/gammaH239R eliminated Ni(2+)

34 This double mutation also creates a binding site for the tran

35 The D58G/D65G double mutation also failed to stimulate CYP2C19-catalyz

36 Vhl/Kif3a double mutation also increased the frequency of cysts th

37 Strikingly, no single or double mutation among the mutations tested had an apprec

38 screen, we identified 22 single mutations, 4 double mutations, and 2 triple mutations of IAPP that ap

39 APP (APP(695)), or APP harboring the Swedish double mutation (APP(swe)) triggers increased ryanodine

40 th conditions, embryos containing homozygous double mutations are lethal, demonstrating that, as expe

41 cted to be common, so we ask here how common double mutations are likely to be and why PI3Kalpha with

42 Single mutations can be potent, but double mutations are stronger: their combination is spec

43 , the computational model did predict that a double mutation at i, i+4 positions at the center of the

44 tations affected viral fusogenicity, but the double mutation at Ng2 and Ng5 in HR1 and HR2 increased

45 The frequent double mutation at the -5 and -2 positions moderately re

46 fold specificity enhancement achieved with a double mutation at the loop/active site interface is acq

47 Single and double mutations at residues D239 and F251 were made in

48 Double mutations at Ser(213) and Ser(217) suppressed NFA

49 Results suggest that single and double mutations at T104 and I196 produce a significant

50 Introduction of single and double mutations at the equivalent arginine (Arg(283)) a

51 es Glu724 and Asp725 were most affected with double mutations at these sites showing approximately 10

52 increases, there is a tendency for computed double mutations at these sites to drive CAP toward nonc

53 ation of LQTS, particularly for fetuses with double mutations, at high risk of early life-threatening

54 fied the effects of all single mutations and double mutations between all positions in the IgG-bindin

55 The mdh1Deltaaat1Delta double mutation blocks CR-mediated life span extension a

56 an intermediate phenotype compared with the double mutation, both in terms of surface expression and

57 nhibitory activity against EGFR(L858R/T790M) double mutations but also exhibited tenfold potency bett

58 The fdm1 and fdm2 double mutations but not the fdm1 or fdm2 single mutatio

59 and 110 KCNQ1 mutation carriers, whereof 13 double mutation carriers).

60 orphology was assessed in mutation carriers, double mutation carriers, and nonmutation carriers by ap

61 In contrast, the (P722H and N724K) double mutation causes structural perturbations in TKD,

62 In plants carrying the cca1-11/lhy-21 double mutation, cold induction of CBF1, CBF2, and CBF3

63 Single and double mutation combinations exhibited a decrease in cat

64 The D58G/D65G double mutation competes poorly with wild-type b(5) for

65 Moreover, the double mutation completely switched the semiprocessive m

66 L529 and I544 and particularly the L529 I544 double mutation compromised viral entry and fusion.

67 M154I, as well as T66I-S153Y and T66I-M154I double mutations, confer resistance to diketo acids.

68 Single and double mutations conferred a competitive advantage over

69 f (-D523N, -E529K) and found that single and double mutations conferred mlh3-null-like defects with r

70 A double mutation, D132A/R481K, is even more strongly inhi

71 The double mutation D27H/E28H causes a pH-dependent fraction

72 Despite increasing GD2 affinity, the double mutation D32H (L-CDR1) and E1K (L-FR1) did not fu

73 recombinant vaccinia viruses with single or double mutations (D68N and H181L) in the uracil DNA glyc

74 However, for all three kinases, the double mutation designed to restore the salt bridge inte

75 uencing method, we found that 70% (33/47) of double mutations detected by direct sequencing were comp

76 The NA-F144C/T342A double mutation did not substantially affect virion morp

77 While beta-interface R41E/K42D double mutations did not interfere with dimerization, th

78 Mice with an allelic series of Lis1 and Nde1 double mutations displayed a striking dose-dependent siz

79 A form of the SRF gene with a double mutation (dmSRF) was generated.

80 n which NA carries the mutation E119V or the double mutations E119V I222V) have similar in ovo growth

81 rgest increase in current is produced by the double mutation E1D and R1S.

82 ctrostatic interaction was eliminated by the double mutation E403C + E758C, the kinetics of recovery

83 In sharp contrast, the b5 double mutation E48G/E49G did not form cross-linked comp

84 s study we explore the mechanisms by which a double mutation (E59D/D75Y) in cardiac troponin C (CTnC)

85 s extracellular domain, the CaS(S875A/T888A) double mutation elicited maximal activation even under c

86 ions of pericentromeric H3K9me levels, but a double mutation eliminates this mark.

87 rk phosphorylation and a mimicking S405,418D double mutation enhanced cortactin binding and activatio

88 Several other combinations of double mutations, especially sigE sigF, caused a transie

89 f magnitude, whereas the Tyr166Phe/His252Ala double mutation exhibited 4.4% of the wild-type activity

90 Results from double-mutation experiments further demonstrate that F13

91 The electrical properties of the single and double mutations further indicate a possible interaction

92 the different medical cohort, patients with double mutation had worse 3-year OS of 18%, compared wit

93 Immunostaining revealed that smyd1a; smyd1b double mutations had no effect on myosin gene expression

94 Parasites with the double mutations have both reduced resistance and dimini

95 ents with a single mutation and those with a double mutation; however, FMF patients of both types sho

96 021), RAS mutation (HR 1.74; P = 0.015), and double mutation (HR 3.09; P < 0.001).

97 Double mutation (i.e. S345A/S346A) within this motif abo

98 .5-fold (540 microm), and EchA variants with double mutations, I219L/C248I and F108L/C248I, increased

99 We introduced a double mutation (I38A/I40A) into the N-terminal domain o

100 or loss-of-function have been identified: a double mutation I48D/S49P inactivates MscS, whereas the

101 a form of P-glycoprotein (Pgp1) containing a double mutation, I837L and N839I, in transmembrane domai

102 atrbohD/F double mutations impair ABA-induced stomatal closing, AB

103 nding was eliminated by an E379A-E381A ADPRT double mutation, implying that residual ADPRT activity,

104 The double mutation in Act variant weakens the hydrophobic i

105 mutants was significantly reduced, whereas a double mutation in both motifs abolished the release of

106 We have introduced a single or a double mutation in conserved amino acid residues contain

107 enotypes of hypocotyl vasculatures caused by double mutation in ERECTA (ER) and ER-LIKE1 (ERL1) recep

108 ntly identified ligand-dependent S252L/A315S double mutation in FGFR2 was shown to cause syndactyly i

109 We found that a double mutation in it, R2140A-K2143A, abolishes bending

110 A double mutation in MHD2 (F1000A/K1002A) disrupts the UNC

111 Double mutation in pilQ1 and tonB had no effect on these

112 y, the temporality of an HBV 1762(T)/1764(A) double mutation in plasma and tumors.

113 Double mutation in RAS/TP53, identified in 31.4% of pati

114 lved in the binding of Sam68 to Rev, while a double mutation in Rev, L78D and E79L, like those in the

115 raphical differences were found, including a double mutation in rpoB found in 37.1% of isolates from

116 The double mutation in SCN5A, V232I, and L1308F alters the a

117 tis B virus (HBV) carrying the A1762T/G1764A double mutation in the basal core promoter (BCP) region

118 T758M) that abolish CD98hc association and a double mutation in the corresponding residues in the bet

119 A double mutation in the distal C-terminal CaM-binding sit

120 The constructs carried either an S4S5/S6 double mutation in the first alpha-subunit and no mutati

121 A double mutation in the hallmark GxxG loop (GxxG-to-GDDG)

122 The double mutation in the peptide reduces the interaction w

123 ariant that contains the Lys103Asn/Tyr181Cys double mutation in the RT enzyme.

124 A natural double mutation in this binding site, which changes nucl

125 We next identified single and double mutations in AcrA and MexB that enabled the compl

126 iviruses, viruses carrying complementary the double mutations in both the 5'- and the 3'-CS were indi

127 east Saccharomyces cerevisiae, we found that double mutations in Cdc48 and its adaptor Ubx4 cause mit

128 We identified 2 ET patients harboring double mutations in cis in MPL, namely, L498W-H499C and

129 rmed by introducing the analogous single and double mutations in cotton activase.

130 Single or double mutations in domain I (with the exception of P60/

131 Double mutations in flh-2 and a third FLYWCH Zn-finger-c

132 SSOCIATED1 (NOA1) and completely restored by double mutations in NOA1 and either of the nitrate reduc

133 Mutants carrying double mutations in nonequivalent genes (i.e., motA motD

134 Available data suggests that double mutations in patients with hypertrophic cardiomyo

135 Double mutations in patients with hypertrophic cardiomyo

136 Double mutations in plp and either vah1 or llpA resulted

137 altering replacement, T257S, and analyze the double mutations in several gp120 envelope glycoprotein

138 However, double mutations in SRS 5 and SRS 6 successfully introdu

139 Double mutations in substrate recognition sites (SRS) 1

140 ic genetic mutants, in this case, single and double mutations in synthesis of carbon storage molecule

141 The results indicated that single and double mutations in the 5' end of the PPT had modest eff

142 ns, we identified variants having single and double mutations in the 5'- and 3'-CS components that co

143 nosoma brucei We have designed 26 single and double mutations in the active site, including residues

144 8A, vR129A, and vRR129AA, carrying single or double mutations in the GKYLQRRLQ motif were characteriz

145 We have characterized single and double mutations in the M1-M2 segment of an inwardly rec

146 Four mutants showed closely spaced double mutations in the new sequence, and analysis of th

147 Interestingly, however, double mutations in the plastid K(+) exchange antiporter

148 We used single and double mutations in the pore of the Shaker K(+) channel

149 Double mutations in the t-RNA barrier and these complexe

150 is further supported by the observation that double mutations in TTD that abolished its interaction w

151 s of codon 409 or 411 mutations evolved into double mutations including V353A.

152 e D321A PS mutation, but not the S252L/A315S double mutation, increase the binding affinity of FGFR2c

153 by 4- and 8-fold, respectively, whereas the double mutation increased binding affinity 10-fold.

154 Both double mutations increased downstream phosphorylated Akt

155 for the previously reported mCG-->TT tandem double mutations induced by Fenton-type reagents.

156 siological experiments demonstrated that the double mutations interchange the phosphate and diphospha

157 The introduction of the double mutation into the S2 subsite of cathepsin K rende

158 he N205C/Y266C, F204C/Y266C, and N205C/A265C double mutations into wild-type and P258L/S259L constitu

159 esis is tested by construction of single and double mutations involving the residues of the Quad muta

160 The V82F/I84V double mutation is considered as the key residue mutatio

161 However, the stt3a-1 stt3b-1 double mutation is gametophytic lethal.

162 sence of E2F binding sites, while an E2F+Inr double mutation is not repressed at all by these gene pr

163 that the dominating effect of the V82F/I84V double mutation is to distort the geometry of the bindin

164 e proceeded with a single, fast phase in the double mutation K-1317R, K-1318R.

165 l activity was detected in channels with the double mutation K188A/R189A, a mutant which exhibits ext

166 The most severe effect was caused by a double-mutation, K48A-R49A.

167 A novel and replication-deficient double mutation (L159F/L320F) confers low-level resistan

168 ed by a single mutation, L213Asp --> Ala, or double mutations, L213Asp-L212Glu --> Ala-Ala (AA), the

169 proves catalytic efficiency using Gly, but a double mutation (L487A/P488A) is required to convert the

170 o alanines partially rescued fusion, and the double mutation L539, 548A completely rescued syncytium

171 It was shown previously that the double mutation L55S/L56S in the flexible region of RhoG

172 A double mutation lacking both dronc and strica impaired P

173 Various double mutations largely eliminate both gene conversion

174 the Appswe transgenic mouse model bearing a double mutation leading to higher Abeta production.

175 o address this, we generated transgenic (TG) double mutation mouse lines (alpha-TM DM) expressing mut

176 le: PML-RARA rearrangement (n = 29) or CEPBA double mutations (n = 42; overall survival [OS] at 3 yea

177 Single mutation N663Q and double mutations N348Q/N468Q, N348Q/N812Q, and N468Q/N81

178 Double mutations neutralizing adjacent basic residues at

179 e abolishes I domain activation, whereas the double mutation of alpha(L)-E310C with either beta(2)-A2

180 Survival after hepatectomy in patients with double mutation of APC and PIK3CA and others was analyze

181 Double mutation of APC and PIK3CA predicts inferior resp

182 Forty-five patients had double mutation of APC and PIK3CA; 351 did not.

183 eurons ADF, but neither the mutation nor the double mutation of both channel genes affects other type

184 The double mutation of both residues further compromises cat

185 HSF1, the mutation of C3 (amino acid 153) or double mutation of C4 and C5 (amino acids 373 and 378, r

186 cy to form dimers and ADPase activity, while double mutation of D228T/K224N largely restored the abil

187 demonstrate that expression of a K35A Y235A double mutation of dadX in E. coli suppresses bacterial

188 Null mutation of IRE1, double mutation of ERAD components (hrd1Delta and ubc7De

189 or flh-2 genes result in a viable phenotype, double mutation of flh-1 and flh-2 results in early larv

190 vR mutant phenotypes could be rescued with a double mutation of irvA or complemented by introducing a

191 ons did not affect packaging, a YFC with the double mutation of leucine 81 and valine 88 was nonfunct

192 Consistently, a double mutation of lgtG and misR in strain NMB yielded a

193 omes close to the HMBPP diphosphate, whereas double mutation of nonconserved residues (Ser/Thr(296/29

194 phorylation of Akt (Ser473), indicating that double mutation of PIK3CA and PTEN has an additive effec

195 We characterized a novel double mutation of SCN5A (V232I in DI-S4+L1308F in DIII-

196 We find that individual mutation or double mutation of Ser-1916 or Ser-1943 to alanine poten

197 Single or double mutation of Sp1 binding sites abolished PKG-Ialph

198 Double mutation of the AhR and CREB-binding sites showed

199 However, double mutation of the HD motif only abolished the DNase

200 d Tyr L162 was also implicated in binding by double mutation of the two residues.

201 Double mutation of these sites caused total loss of CKR'

202 Double mutation of TM2 and 7(D79N/N422D) reveals phenoty

203 In an earlier study, a double mutation of two arginine residues located in a sh

204 is the unique phosphorylation site, and that double mutation of tyrosines 19 and 27 to alanine abroga

205 Double mutations of (1038)LL(1039) or (1048)LI(1049) to

206 5a, with potent inhibition against single or double mutations of c-KIT developed in GISTs.

207 However, double mutations of either serines 15 and 20 or serines

208 In addition, double mutations of IRX9 and IRX14 were shown to cause a

209 However, deletions and mutations or double mutations of other transcription factor binding s

210 Single and double mutations of PKC consensus sites reduced Pcyt2alp

211 Double mutations of R95 and R102 lead to desilencing of

212 Double mutations of S-->A at S300 and S302 completely di

213 utationally suggested mutations reveals that double mutations of the leucine residues (L317D+L335D) m

214 Single or double mutations of these residues caused marked reducti

215 Double mutations of these residues increased nuclear CII

216 (POR) in reconstituted assays, the D58G/D65G double mutation, of residues located in a hydrophilic al

217 s, the D321A PS mutation and the S252L/A315S double mutation on FGFR2 ligand binding affinity and spe

218 the present work we study the effect of this double mutation on the conformational and dynamic proper

219 Furthermore, the effects of double mutations on both simulated integration efficienc

220 LCIA compared with the effects of LCIB-LCIA double mutations on growth and inorganic carbon-dependen

221 The effects of double mutations on k(a), k(lim), and k(app) were small

222 The double mutation, on the other hand, seems to cause a maj

223 o CTG codons either directly by simultaneous double mutations or indirectly through an intermediary c

224 ype RAS (OR 2.00; P = 0.003), and absence of double mutation (OR 2.91; P = 0.002).

225 naling is strongly disrupted by Hs2st; Hs6st double mutation, or by overexpression of 6-O sulfatase,

226 3-year OS of 18%, compared with 35% without double mutation (P = 0.023).

227 gle mutations (P64, P67, P70, and P71 to A), double mutations (P64/67A, P64/70A, and P67/70A), and tr

228 The F39A/L12F double mutation partially regained the affinity for DsrA

229 paired the formation of D'D3 dimers, and the double mutation prevented dimerization.

230 (alpha1Q241L mutation and alpha1N407A/Y410F double mutation) produce similar effects on macroscopic

231 utations (K104A, K105A, and E106A) and three double mutations (R101A/K102A, K104A/K105A, and K102A/R1

232 In contrast, the double mutation R101S-R105S was synergistic and resulted

233 more weakly to FliI carrying the N-terminal double mutation R7C/L12P than it did to wild-type FliI,

234 to the terminal phosphodiester; a S37A-T80A double mutation reduced kinase activity 50-fold.

235 alpha-interface K101D/R102E double mutations reduced dimerization but only slightly

236 Importantly, the pawh1 pawh2 double mutation reduces the protein abundance of EBS7 an

237 e chains is essential per se, an N140A/T142A double mutation reduces the rate of supercoil relaxation

238 ions are likely to be and why PI3Kalpha with double mutations responds effectively to inhibitors.

239 l with the corresponding V558Delta;V653A Kit double mutation restricted (a) spatially to ETV1(+) cell

240 The Y185F/E272Q double mutation resulted in a synergistic decrease in th

241 Furthermore, the cca1-11/lhy-21 double mutation resulted in impaired freezing tolerance

242 nvestigation showed that the dgat1-1 pdat1-2 double mutation resulted in sterile pollen that lacked v

243 Previous studies have shown that this double mutation results in replacement of the wild-type

244 and further investigations revealed that the double mutation results in the arrest of embryo developm

245 In sharp contrast, the D58G/D65G double mutation retained the full ability to stimulate t

246 The double mutation S184A/S374A caused an increase in MIPS a

247 The double mutation S503A/Q504L increased the affinity for A

248 he Y501DSQ loop, mutations S503A, Q504L, and double mutation S503A/Q504L, was made to test the predic

249 The double mutation S729T/N732K rendered Na(v)1.4 more "brai

250 nt for nuclear accumulation, as shown by the double mutation S883D/nuclear localization signal.

251 Single mutations in four amino acids or double mutations (serines 276 and 293 or threonine 300 a

252 It has been shown that the to double mutation severely affects the viability of bacter

253 ely block DNA binding whereas an S163E/S177E double mutation severely inhibits DNA binding.

254 Only the presence of a CEBPA-double mutation should be used for therapy risk stratifi

255 We expressed a double mutation shown previously to interfere with lipid

256 k2Delta, enhanced TPE, whereas the effect of double mutation, sir1Delta hek2Delta, did not exceed tha

257 and 30-fold, respectively, and a K133A/K135A double mutation slows relaxation and cleavage by 120- an

258 tallization strategy in which the G65R/K206E double mutation stabilizes a single closed pink conforme

259 ytoin 4-hydroxylation, whereas the E48G/E49G double mutation stimulated these activities of CYP2C19 a

260 gly, wild-type and attenuated (by single and double mutations) strains of S. enterica serovars Typhim

261 We show that neither single nor double mutations structurally perturbed recombinant prot

262 Mice with AIRE/Foxp3 double mutations suffer from exacerbated autoimmunity wh

263 lly the same for either mutation, or for the double mutation, suggesting that the alpha beta dimer be

264 The double mutation suppresses myofibril assembly defects in

265 The double mutation swapping Asp(203) and Arg(678) rescues A

266 lation, whereas this is not the case for the double mutation swapping Ser(186) and Glu(439).

267 p120 glycoproteins with the T257S-plus-S375W double mutation (T257S+S375W) have a superior antigenic

268 The double mutation T381A-V382P, which was predicted by mode

269 The double mutation T381V-V382P, on the other hand, did not

270 ions significantly reduces repression, and a double mutation that prevents interaction with both CtIP

271 A double mutation that removes the disulfide bridge betwee

272 educe the enzyme activity and finally design double mutations that are aimed at restoring the activit

273 However, double mutations that deleted the hydroxyl group from th

274 tic and structural effects of the T97I/P101S double mutation, the molecular basis for GA21 corn, usin

275 probes for detecting consecutive nucleotide double mutations, the kinetics and thermodynamics aspect

276 pondingly enhanced cADPR production, and the double mutation, Thr-221 to Phe and Glu-146 to Ala, effe

277 loid beta-protein precursor with the Swedish double mutation, to C57BL/6 mice, a strain susceptible t

278 te C32 or C35 (Trx-C32S or Trx-C35S) and the double-mutation (Trx-CS).

279 On multivariable analysis, RAS/TP53 double mutation was an independent predictor of shorter

280 The prognostic value of double mutation was evaluated with a separate cohort of

281 This double mutation was previously found to be associated wi

282 llen tube growth in syp124/syp125 single and double mutations was quantitatively predicted based on t

283 cell line stably expressing an AD-associated double mutation, we report that EGb761 inhibits formatio

284 anning analysis of 81 single and 32 pairwise double mutations, we show that the hormone's two spatial

285 GISTs driven by the V558Delta;V653A Kit double mutation were resistant to imatinib, while caboza

286 Both single and double mutations were constructed to study the molecular

287 Single and double mutations were made in the fluorophore of EGFP to

288 When double mutations were present, the conformational equili

289 responding to the supply rates of single and double mutations, where mu denotes the mutation rate.

290 ciency virus type 1 (HIV-1), the C(377,409)S double mutation, which disrupts both CCHC zinc fingers i

291 processing were phenotypically silent, but a double mutation, which further increased the rate of bou

292 s containing Y181C-, K103N-, and K103N-based double mutations, which account for a significant propor

293 A double mutation with a putative plastid ADS3 paralog exa

294 ing activities is not as severe as R70A, its double mutation with Asp-181 had a synergistic effect.

295 that a recombinant virus (YD12) containing a double mutation within conserved exonuclease motif III o

296 A number of double mutations within helices 3, 9, and 10 reduced pho

297 Clonal sequencing identified single or double mutations within the 3CLpro coding sequence of in

298 ne mutant enzyme species that have single or double mutations within this putative internal propeptid

299 Interestingly, the double mutation Y(747)F/Y(759)F, which disrupts platelet

300 s of biochemical experiments showed that the double mutations Y120F/Q125H, but not either single muta