1 n VLM and NTS, 74% and 42% respectively were
double labeled.
2 Significantly, very few neurons (<2%) were
double-labeled.
3 n is deuterated and the C-terminal domain is
double-labeled.
4 enalidomide treatment using CD138(+)/IRF4(+)
double labeling.
5 he IC and auditory cortex did not reveal any
double labeling.
6 ected to both the AI and the LO as judged by
double-labeling.
7 on the synthesis and use of an isotopically
double-labeled Abeta1-15 glycopeptide, carrying the core
8 oat protein gene of CTV (CTV-p25) and detect
double labeled amplicons on a sandwich immunoassay by de
9 assay was performed with a primary antibody,
double-labeled amplicons, and fluorophore-labeled strept
10 Our
double-labeling analyses of intraneuronal Abeta and CD40
11 Finally,
double-labeling analysis of CTb + Fos showed that contex
12 Evidence from both
double label and single axon studies, however, suggests
13 Combining
double labeling and confocal microscopy analyses, we fou
14 Fifteen of these were confirmed with
double labeling and microscopy, including the transcript
15 otope probing (Raman-SIP) using a single and
double labeling approach.
16 nstrated by cell lineage tracing and mineral
double-labeling approaches that murine PDL progenitor ce
17 Hyperpolarized,
double-labeled aspirin was well tolerated in mice and co
18 hree taste cell types expresses this enzyme,
double-label assays were performed with antisera directe
19 Some
double-labeled BDA/VGAT varicosities were seen apposed t
20 content and the numbers of insulin and BrdU
double-labeled beta cells in the islets.
21 To prove this hypothesis, we tracked
double-labeled BMSCs in implantation sites created in nu
22 Some BrdU-labeled cells were also
double labeled by antibodies to glial-specific (antikera
23 When
double labeled by in situ hybridization, these neurons c
24 18-19%) of dorsal root ganglion neurons were
double labelled by dye tracers injected into either blad
25 Double-labeled cancer cells were imaged at the cellular
26 treated animals by the increasing number of
double labeled CD68+/CD206+ cells in the cranial and cen
27 nt topographically organized zones, with few
double-labeled cells (~4-6%).
28 Double-labeled cells are injected by various methods.
29 hat, for all three primary sensory cortices,
double-labeled cells were extremely rare, indicating tha
30 Generally,
double-labeled cells were in expected high-frequency ton
31 abeled cells in LH co-expressed SS, while no
double-labeled cells were observed in IC.
32 Elsewhere,
double-labeled cells were very scarce, making up approxi
33 cells, there is a considerable population of
double-labeled cells with approximately 30% of each popu
34 ess can be observed to emanate from BrdU/DCX
double-labeled cells.
35 To do this, we used
double-labeling combinations of Fos immunocytochemistry
36 melatonin receptor localization in the OPL,
double-label confocal immunohistochemistry for Mel1a or
37 Extensive
double-labeling crossover studies have been conducted.
38 e mechanism was obtained through an isotopic
double-labeling crossover study.
39 g to bones through the use of differentially
double-labeled derivatives.
40 BrdU staining, providing a valuable tool of
double labeling DNA synthesis, e.g., for tracking the tw
41 Conventional molecular beacons require
double-labeled DNA sequences, which are costly and time-
42 ch DNA molecule, thereby establishing short,
double-labelled DNA molecules as probes of 3D orientatio
43 lowing the principle of proportionality, and
double-labeled duplex DNA was synthesized.
44 Double label experiments showed that all orcokinin-immun
45 Double label experiments with b-sCD44 or -p b-sCD44 and
46 ochemical methods to test the feasibility of
double labeling experiments during development.
47 Double labeling experiments indicate that the neurochemi
48 Double labeling experiments, by either in situ hybridiza
49 bone formation rates in in vivo fluorochrome
double labeling experiments.
50 Double-label experiments did not confirm colocalizations
51 Double-label experiments showed that the SIFamide-immuno
52 Double-label experiments showed that these bipolar cells
53 Double-labeling experiments (biocytin backfill x seroton
54 Double-labeling experiments confirm that NADPH-d, the mo
55 Double-labeling experiments further demonstrate that V1
56 c gut-associated lymphoid tissue at 14 days;
double-labeling experiments indicated that 93.5% of the
57 Double-labeling experiments reveal NMB is expressed in a
58 Double-labeling experiments showed that the dendritic an
59 in the control of food intake, we performed
double-labeling experiments to evaluate the potential ov
60 Double-labeling experiments using probes for previously
61 Single- and
double-labeling experiments were performed to establish
62 Double-labeling experiments with ArINTs and ArINTstBu (T
63 When mAb-GlyRbeta was used in
double-labeling experiments with GlyRalpha1-, alpha2-, a
64 y 15, we assessed PKCdelta and SOM, Fos, and
double-labeled expression in CeL and central amygdala me
65 then determining the relative proportions of
double-labeled FF and FB neurons in an area intermediate
66 the lateral septum (LS), whereas only a few
double-labeled fibers were found in other brain areas in
67 Double-label fluorescence for Abeta42 and pT231 revealed
68 Rats had
double-label fluorescence IMHC for localization of Abeta
69 Double-label fluorescence in situ hybridization reveals
70 Using
double labeling fluorescent histochemistry with confocal
71 Analysis of
double-label fluorescent images showed a decline in Iba1
72 Third, using the technique of
double-labeled fluorescent immunocytochemistry, the rela
73 amined using myeloperoxidase (MPO) assay and
double-labeling fluorescent immunohistochemical analysis
74 They were also
double labeled for GFP and RPE65 or MITF.
75 As expected,
double labeling for ATF-3, a marker of cell bodies with
76 Double labeling for GAD67 and GHRHR in vitro and in vivo
77 The current investigation used
double labeling for NADPHd and Fos-like immunoreactivity
78 Double labeling for Prox1 and cell-type-specific markers
79 bserved in dendritic profiles, verified with
double labeling for the dendrite-specific marker microtu
80 al cells were identified as keratinocytes by
double labeling for WNV antigen and keratin 10.
81 The proportions of TH cell profiles
double-labeled for ChAT or VIP significantly increased b
82 -estradiol, the number of perirhinal neurons
double-labeled for ER-beta/GABA was reduced by 28% (P<0.
83 ctory organs were cryosectioned (10 microm),
double-labeled for Galpha(olf), Galpha(q), or PLC140, an
84 cells in the inner nuclear layer (INL) were
double-labeled for TH immunoreactivity.
85 The majority of FG-filled cells
double-labeled for TH were found in the dorsocaudal A10
86 Double-labeling for GAD and synaptophysin confirmed that
87 samples that are generally different from a
double-labeled FRET sample, or by the use of sophisticat
88 Double labeling further reveals that the majority of Cck
89 Light and electron microscopic
double labeling further showed that the VGLUT1 subtype o
90 cumented using the characteristic pattern of
double-labeled gene trees.
91 ive glycoproteomic approach described, using
double-labeled glycopeptide standards, will undoubtedly
92 and protein immunofluorescence (IF)-RNA FISH
double labeling (
IF/FISH).
93 Double-label IHC experiments with tyrosine hydroxylase a
94 Using
double-labeled immune-fluorescence confocal microscopy,
95 Double labeling immunocytochemical studies confirmed tha
96 using retrograde tract tracing combined with
double label immunocytochemistry and found that neuron p
97 specimens were analyzed by Western blot and
double label immunocytochemistry.
98 Double-label immunocytochemistry detected EAAT2 in OLs b
99 epileptic cortical samples were analyzed by
double-label immunocytochemistry for coexpression of neu
100 Single- and
double-label immunocytochemistry showed a significantly
101 We utilized
double-label immunocytochemistry to identify the phenoty
102 We applied single- and
double-label immunocytochemistry to normative frontal or
103 alogs and also determined their phenotype by
double-label immunocytochemistry using type-specific mar
104 e cell populations and connectivity, we used
double-label immunocytochemistry with antisera to differ
105 ped in a teleost (zebrafish, Danio rerio) by
double-label immunocytochemistry.
106 Double label immunofluorescence analysis of 3T3-L1 adipo
107 hat controls feeding and energy expenditure,
double label immunofluorescence studies were employed.
108 by using a combination of routine histology,
double-label immunofluorescence and in situ hybridizatio
109 Double-label immunofluorescence confocal studies show th
110 yelin unwinding and intramyelinic cysts, and
double-label immunofluorescence for 4-hydroxy-2-nonenal
111 Double-label immunofluorescence for BrdU and the protein
112 alpha-smooth muscle actin(+)) as assessed by
double-label immunofluorescence microscopy.
113 of hypocretin neurons was analyzed by using
double-label immunofluorescence of FosB/DeltaFosB with h
114 esame oil-treated animals were processed for
double-label immunofluorescence of GAD (a marker for GAB
115 Double-label immunofluorescence staining demonstrated th
116 In
double-label immunofluorescence staining, CD11c+, a mark
117 We used immunohistochemistry and
double-label immunofluorescence to characterize CD163(+)
118 We used
double-label immunofluorescence to visualize the distrib
119 Examining SIVE lesions, using
double-label immunofluorescence with antibodies against
120 Using
double-label immunofluorescence, GLT-1 was shown to colo
121 Using
double-labeling immunofluorescence analysis of intraneur
122 Double-labelling immunofluorescence showed a 95% co-loca
123 With
double-labeling immunogold electron microscopy (EM), we
124 Two
double-label immunohistochemical experiments were conduc
125 Double-label immunohistochemical procedures demonstrated
126 Double label immunohistochemistry revealed that hemorrha
127 Using
double label immunohistochemistry we found that DsRed fl
128 We demonstrate using
double labeling immunohistochemistry that Group II metab
129 of GLP-1R expressing cells in the CNS, using
double-label immunohistochemistry and in situ hybridizat
130 of stress-responsive LC neurons we performed
double-label immunohistochemistry for TH and Fos.
131 d precursor protein (APP) has been observed,
double-label immunohistochemistry revealed no evidence o
132 Double-label immunohistochemistry revealed that acute re
133 In the present study, we used single- and
double-label immunohistochemistry to investigate the rel
134 Double-label immunohistochemistry using antibodies again
135 tacts between identified cell types, we used
double-label immunohistochemistry, in vivo retrograde tr
136 By
double-label immunohistochemistry, we found close apposi
137 of AD and control patients using single- and
double-label immunohistochemistry.
138 utamate) immunoreactivity (FOS/PAG-IR) using
double-label immunohistochemistry.
139 ne (BrdU) labeling of newly generated cells,
double-labeling immunohistochemistry and TUNEL labeling
140 Double-labeling immunohistochemistry indicated that PR-i
141 Double-labeling immunohistochemistry was performed with
142 Double-labelling immunohistochemistry and confocal micro
143 ing to identify corneal afferent neurons and
double label in situ hybridization and/or immunohistoche
144 Protein
double labeling in aqueous buffer at physiological pH, t
145 lateral lemniscus (VNLL and INLL), with some
double labeling in ipsilateral lateral and medial superi
146 ents to clarify the causes of BrdU-gammaH2AX
double labeling in mouse and human beta-cells.
147 tion neurons exhibited a higher incidence of
double labeling in the superficial dorsal horn.
148 s before sacrifice, we have used single- and
double-label in situ hybridization.
149 r postnatal stages of C57BL/6J mice by using
double-label in situ hybridization.
150 e projecting to accumbens shell, with higher
double-labeling in the ipsilateral projection than in th
151 In both species, lack of overt
double labeling indicated that the ChAT(+) and urocortin
152 Less than 1% of cells were
double-labeled,
indicating that the populations of cells
153 Mouse LGs were processed for single- and
double-labeled indirect immunofluorescence studies and e
154 Different sets of
double labels initially located 26-27 A apart in the min
155 When duplex TAR DNA-TAR RNA formed,
double labels initially located 27.5 A apart at the 3'-
156 We
double labeled Ipc axons and their presumptive postsynap
157 now applied single-molecule FRET to Cy3, Cy5
double-labeled LacI-DNA loops diffusing freely in soluti
158 In the absence of complementary TAR RNA,
double labels located in both the upper and the lower st
159 of fine processes called telodendria and, in
double-labeled material, Cx36 plaques were located preci
160 study femurs were isolated from genetically
double-labeled mBSP9.0Luc/beta-ACT-EGFP transgenic mice
161 We employed a
double-label method to map the ascending projections of
162 ural level, we develop and apply a "two-tag"
double-labeling method to label LT11's dendrites and the
163 eparate populations of synapses, we employed
double-labeling methods.
164 f these compounds was readily converted to a
double-labeled mixed-chain phosphatidylcholine applicabl
165 abeled motoneurons and higher proportions of
double-labeled motoneurons than untransected rats.
166 The distance distributions between three
double-labeled mutants, in the collapsed transient state
167 co-expressed enkephalin (Enk), and Ucn 3/Enk
double-labeled nerve fibers and terminals were observed
168 ope, and scored for the number of single and
double labeled neurons.
169 ed with no laminar segregation, we found few
double-labeled neurons ( approximately 5% of each singly
170 However,
double-labeled neurons also exhibited a preferential dis
171 fferent tracers were injected in SII and MI,
double-labeled neurons appeared above and below the laye
172 tral PBN resulted in a greater percentage of
double-labeled neurons in BNST and CeA compared to cauda
173 d in significant increases in the numbers of
double-labeled neurons in both the NST and RF, suggestin
174 These differential distributions of
double-labeled neurons in the NST and RF suggest a role
175 Double-labeled neurons in the NTS were located primarily
176 Minor PACAP projections with scattered
double-labeled neurons originated from the parabrachial
177 ime-lapse confocal microscopy of individual,
double-labeled neurons revealed a coincident, activity-d
178 sker representations of SII and MI to detect
double-labeled neurons that would indicate that some SI
179 of cortical GABA, ER-beta, and ER-beta/GABA
double-labeled neurons was examined.
180 For SS, the percentage of
double-labeled neurons was more forebrain site specific
181 Sparse populations of
double-labeled neurons were found in both V1 and V2 but
182 Double-labeled neurons were found in the reticular, raph
183 Overall,
double-labeled neurons were most numerous in the caudal
184 In the NST,
double-labeled neurons were most numerous in the rostral
185 Moderate numbers of
double-labeled neurons were observed following combined
186 However, only a few
double-labeled neurons were occasionally observed after
187 ric acid stimulation, there was a cluster of
double-labeled neurons with distinctive large soma in th
188 response to quinine, there was a cluster of
double-labeled neurons with much smaller soma in the int
189 ese animals was examined for the presence of
double-labeled neurons, i.e., those whose axons had rege
190 trogen-mediated differences in the number of
double-labeled neurons.
191 NST and CeA exhibited significant numbers of
double-labeled neurons.
192 d neurons in ipsilateral DEn, including many
double-labeled neurons.
193 colocalized and contained some percentage of
double-labeled neurons.
194 ed neurons in the claustrum, as well as many
double-labeled neurons.
195 eased at mRNA levels in bladder afferent and
double-labelled neurons vs. non-labelled neurons, and sl
196 a tripeptide tag CIS that allows facile N, S-
double labeling of a protein of interest with >90% yield
197 We hypothesized that the
double labeling of an adenovirus with fluorescent protei
198 Double labeling of CB2R and glutamine synthetase shows t
199 4-5-day hatchling chicks by using single and
double labeling of fibers and terminals with biocytin co
200 al surgery activated brain CRF neurons using
double labeling of Fos/CRF in naive rats.
201 The
double labeling of ganglion neurons indicates their site
202 Double labeling of JO and ocellar afferents revealed tha
203 a coli OM B(12) transport protein, BtuB, the
double labeling of many cysteine pairs is not possible i
204 Indeed,
double labeling of miR156 showed a meristem-specific pat
205 Transgenesis and
double labeling of NTS and HCRT neurons showed that NTS
206 Double-transgenic mice showed 55%
double labeling of periurethral neuroendocrine cells exp
207 ectly in blood and plasma, demonstrating the
double labeling of platelet EVs with CD61 and CD9, as we
208 rviving ganglion cells were quantified after
double labeling of retinal tissue with TUNEL and Brn3a.
209 incorporated at distinct sites, enabling the
double labeling of ScFv with distinct probes, through mu
210 Double labeling of sGC with neuronal nitric oxide syntha
211 Moreover, we demonstrate that the
double labeling of sites on the periplasmic-facing surfa
212 g context-induced renewal tests by measuring
double labeling of the retrograde tracer cholera toxin s
213 Double labeling of TSH4 with the ramosa2, branched silkl
214 Although
double-labeling of microglia with Iba1 and ED1 revealed
215 Double-labeling of neurons in which GFP was driven by C3
216 First, we measured
double-labeling of the neuronal activity marker Fos with
217 pha-CreER(T2) : R26R-YFP transgenic mice, we
double labeled OPCs and traced their fate in the postnat
218 process through crossover experiments using
double-labeling (
oxo and phosphine).
219 The
double-labeled peptides have amide I' IR spectra that sh
220 From triple-labeled tissue, we found that
double-labeled PHA-L (+)/VGluT2 (+) axon terminals forme
221 These mGluR1alpha
double-labeled populations are not likely to overlap sin
222 A dense plexus of
double-labeled prodynorphin/proNKB-ir fibers was found w
223 nation of methods allows for rapid access to
double-labeled proteins with a minimum of unnecessary ch
224 We have used both single- and
double-label protocols to investigate the relation betwe
225 triple-labeled PV(+) /CR(+) /SMI32(+) ; (b)
double-labeled PV(+) /CR(+) ; and (c) single-labeled CR(
226 movement was quantified by image analysis of
double-labeled retinas examined with confocal microscopy
227 Double-label retrograde anatomical tracing techniques we
228 Double labeling revealed that 30.2% of nodose neurons ex
229 Double-labeling revealed that only two regions of the mo
230 By means of
double-labeling RNA in situ hybridization in mice, we sh
231 n a wide system of conditions using the same
double-labeled sample from which the FRET efficiency its
232 Analysis of the
double-labeled sections indicates that NTPDase2 immunore
233 alitative abnormalities, with bone turnover (
double labeling)
seen in all specimens.
234 Double labeling showed that neuronal nitric oxide syntha
235 Double labeling showed that the thalamic valop populatio
236 Double labeling showed that type 5b cone bipolar cells e
237 Double labeling showed these to be both astrocytes and o
238 r, immunofluorescence of BRCA1 and nucleolin
double-labeling showed colocalization in both nucleoli a
239 Double labeling shows coincidence of STIM1 and STIM2 wit
240 Double labeling shows that primarily separate population
241 gher density of synaptic PKMzeta labeling in
double-labeled spines correlated with both faster task a
242 These
double-labeled spines had larger synapses, as measured b
243 Within this population of
double-labeled spines, aged monkeys compared with young
244 Here, we used a
double-labeling strategy (varying both the distance betw
245 Immunofluorescent
double labeling studies additionally reveal the prominen
246 Double labeling studies confirmed that the alpha-syn::GF
247 Double labeling studies showed that nicotine induced c-f
248 Immunohistochemical
double labeling studies with YFP and serotonin antisera
249 Double-label studies show that all cells immunoreactive
250 Double-label studies showed these inputs ended directly
251 Double-labeling studies demonstrated the localization of
252 Double-labeling studies revealed that early OL progenito
253 In
double-labeling studies using confocal microscopy, fluor
254 Double-labeling studies with antibodies to phosphorylate
255 coefficients (slopes) between serum PTH and
double-labeled surface (P=0.02) or osteoblast surface (P
256 rmone (PTH), which is indicated by decreased
double-labeled surface and osteoblast surface (P<0.001).
257 Immunohistochemical
double-labeling technique with Fos and markers for norad
258 Y-NB (Lucifer yellow-Neurobiotin) retrograde
double-labeling technique, in conjunction with specific
259 Immunohistochemistry performed using
double labeling techniques with several neuronal markers
260 This was investigated with immunofluorescent
double-labeling techniques to coregister PV- and CB-expr
261 Using
double-labeling techniques, we established that these in
262 Double labeling the brainstem sections revealed that irI
263 ons of the RVM and caudal pons and performed
double labeling to evaluate the expression of alpha-7 an
264 En face
double labeling using Ki-67 and progenitor markers revea
265 ions were processed for immunohistochemistry/
double labeling using patient sera/cerebrospinal fluid a
266 la, and midbrain as CRH neurons, although no
double labeling was found.
267 Much less
double-labeling was associated with injections into eith
268 function (bone formation) after tetracycline
double-labeling was performed by fluorescence microscopy
269 Commissural divergence, assessed by
double labeling,
was less than 3% in each area.
270 ot in the MICG, and some of these cells were
double labeled with an antiserum to the glial protein S-
271 readily identified in the aorta and could be
double labeled with antibodies to CD11c and antigen-pres
272 Brains were
double labeled with fluorescence in situ hybridization o
273 tures that in the human VZ/SVZ, cells can be
double labeled with RG markers and calretinin (CalR) and
274 Cell death was quantified by
double labeling with a membrane impermeable dye and 4',6
275 Double labeling with anti-PECAM1 antibody and anti-place
276 Double labeling with anti-PECAM1 antibody and one of thr
277 ype of BrdU-positive cells was identified by
double labeling with antibodies to neuronal or glial mar
278 In
double labeling with calretinin and parvalbumin, few neu
279 We used
double labeling with fluorescent retrograde tracers to i
280 ricular nucleus of the hypothalamus (PVH) by
double labeling with markers expressed in viruses inject
281 Double labeling with neuropeptide Y (NPY), a marker for
282 Double labeling with other molecular markers confirmed t
283 Double labeling with parvalbumin antibodies in monkey re
284 Double labeling with SNAP25 and calbindin antibodies dem
285 Double labeling with the ganglion cell marker RBPMS demo
286 esence of small, clear synaptic vesicles and
double labeling with the presynaptic markers synaptophys
287 Double labeling with the vesicular glutamate transporter
288 o functional recovery, sequential retrograde
double labeling with two fluorescent dextran amines was
289 Double labeling with various glutamate receptor subunit
290 , ii) single-stained with anti-APP, and iii)
double-labeled with anti-APP and AT8.
291 rols (n=5), c-Fos-positive cells and neurons
double-labeled with c-Fos and beta-endorphin, enkephalin
292 Moreover, neurons
double-labeled with c-Fos and choline acetyltransferase
293 on (n=5), c-Fos immunoreactivity and neurons
double-labeled with c-Fos and either enkephalin or 5-HT
294 ulation), c-Fos immunoreactivity and neurons
double-labeled with c-Fos, an immediate early gene and t
295 % of the remaining Rbpms-positive cells were
double-labeled with FG.
296 Immunohistochemistry was performed by
double-labeling with anti-human MMP1 and collagen type I
297 Double-labeling with CTB and NPY was observed in the Arc
298 t 14.6% of CT-beta-labelled PVN neurons were
double-labelled with AVP.
299 Double labeling (
with NeuN and GFAP) immunohistochemistr
300 pale and thick stripes; 10-27% of cells were
double labeled,
with most located in interpatches.