ライフサイエンスコーパス: duramycin

1 (formerly YbfM) have increased resistance to duramycin.

2 ox status of the lung tissue whereas (99m)Tc-duramycin, a new marker of cell injury, senses cell deat

3 Duramycin, a peptide that binds phosphatidylethanolamine

4 s) mutant became extremely hypersensitive to duramycin, although the sensitivity to papuamide A was u

5 ven the unique physicochemical properties of duramycin and the availability of PtdE in acute cell dea

6 this study was to develop and evaluate 99mTc-duramycin as a novel molecular probe for imaging PtdE.

7 high-dose radiation exposure, using (99m)Tc-duramycin as a phosphatidylethanolamine-binding radiopha

8 e potential use of (99m)Tc-HMPAO and (99m)Tc-duramycin as redox and cell-death imaging biomarkers, re

9 on, followed by a gradual decline in (99m)Tc-duramycin binding accompanied by extensive thymic atroph

10 Finally, 99mTc-duramycin binding to acute cell death in vivo was demons

11 motherapy and radiotherapy increased (99m)Tc-duramycin binding to COLO205 cells in a concentration/do

12 Duramycin binds phosphatidylethanolamine (PtdE) at a 1:1

13 enously with either (99m)Tc-HMPAO or (99m)Tc-duramycin (both 37-74 MBq), and planar images were acqui

14 We demonstrated that (99m)Tc-duramycin can be used to image induction of cell death e

15 (99m)Tc-duramycin cell binding and the levels of cell death were

16 apoptosis was assessed using (99m)Tc-linear duramycin control radiotracer.

17 Blocking with unlabeled duramycin demonstrated specific binding of the radiotrac

18 PE-specific cyclic peptide lantibiotic agent Duramycin efficiently inhibits the entry of West Nile, d

19 g toxins that target PS (papuamide A) or PE (duramycin) exposed in the extracellular leaflet.

20 The specificity of (99m)Tc-duramycin for apoptosis was assessed using (99m)Tc-linea

21 , our aim was to validate the use of (99m)Tc-duramycin for imaging the early response of tumors to tr

22 Intravenously injected 99mTc-duramycin has favorable pharmacokinetic and biodistribut

23 (99m)Tc-duramycin holds promise as a noninvasive imaging radiotr

24 The specific uptake of 99mTc-duramycin in apoptotic cells, compared with that in viab

25 (99m)Tc-duramycin is a SPECT tracer for cell death imaging.

26 99mTc-duramycin is a stable, low-molecular-weight PtdE-binding

27 99mTc-duramycin is completely unmetabolized in vivo, and the i

28 Duramycin is covalently modified with succinimidyl 6-hyd

29 Sensitivity to duramycin is restored by expression of B. subtilis PetA

30 The inhibitory effect of Duramycin is specific: it inhibits TIM1-mediated, but no

31 With only 19 amino acids, duramycin is the smallest known polypeptide that has a d

32 (99m)Tc-duramycin lung uptake was also maximal at 7 d of exposur

33 Analysis of duramycin-mediated killing upon induction of PE synthesi

34 Planar whole-body (99m)Tc-duramycin scanning (n = 4 per time point) was conducted

35 We have demonstrated that (99m)Tc-duramycin specifically accumulates in apoptotic tumors i

36 onclusion: We have demonstrated that (99m)Tc-duramycin specifically accumulates in apoptotic tumors i

37 Therapy response was evaluated by (99m)Tc-duramycin SPECT 24 h after the last dose of therapy.

38 response evaluation was assessed by (99m)Tc-duramycin SPECT and (18)F-FDG PET imaging in treatment-s

39 response evaluation was assessed by (99m)Tc-duramycin SPECT and (18)F-FDG PET imaging in treatment-s

40 Here, using the antimicrobial peptide duramycin that targets outward-facing PE, we show that B

41 epatic background, the avid binding of 99mTc-duramycin to the infarcted myocardium quickly becomes co

42 tment, indicating specificity of the (99m)Tc-duramycin tumor signal.

43 No change in (99m)Tc-linear duramycin uptake could be detected in COLO205 tumors aft

44 In vivo, (99m)Tc-duramycin uptake in COLO205 xenografts was increased 2.3

45 After tumor irradiation with 4.5 Gy, (99m)Tc-duramycin uptake in tumors increased significantly (1.24

46 and 3 wk after receipt of 15 Gy, and (99m)Tc-Duramycin uptake was more than doubled at 2 and 3 wk.

47 Results: COLO205 tumor uptake of (99m)Tc-duramycin was increased 7-fold from baseline in conatumu

48 COLO205 tumor uptake of (99m)Tc-duramycin was increased 7-fold from baseline in conatumu

49 Finally, using fluorescently labeled duramycin we demonstrate that cells lacking PetA have re

50 armacokinetics, and biodistribution of 99mTc-duramycin were measured in rats.

51 A second probe, (99m)Tc-labeled Duramycin, which binds to apoptotic cells, was used to m

52 HYNIC-derivatized duramycin with 1:1 stoicheometry was synthesized and con

53 ve power of cell death imaging using (99m)Tc-duramycin with the current gold standard (18)F-FDG for t