ライフサイエンスコーパス: ecto-ATPase

1 o identify non-cross-linked and cross-linked ecto-ATPase.

2 200 kDa band indeed represents the trimeric ecto-ATPase.

3 ecto-ATPase homooligomers would inhibit the ecto-ATPase.

4 adenosine A(1) receptors, ATP receptors, and ecto-ATPase.

5 ted by the corresponding region of the human ecto-ATPase.

6 ockers of nitric oxide, GABAA, glutamate, or ecto-ATPase.

7 a cells confirm that the new gene encodes an ecto-ATPase.

8 bile acid transport mechanism distinct from ecto-ATPase.

9 le library to identify the cDNA encoding the ecto-ATPase.

10 new opportunity to identify the canalicular ecto-ATPase.

11 more potent inhibitor of ecto-apyrases than ecto-ATPases.

12 eins, is observed only in the membrane-bound ecto-ATPases.

13 In the sequence of the CR1 of human ecto-ATPase, 58WPADKENDTGIV69, 65DTG67 is similar to the

14 In addition, cellular ecto-ATPase activities were measured following prolonged

15 cells transfected with CD39 possess similar ecto-ATPase activities, further supporting the conclusio

16 in aggregation is distinct from its role in ecto-ATPase activity and the aggregating properties cann

17 s was developed to explain the modulation of ecto-ATPase activity by a variety of agents, including d

18 and P2X receptors reduced-and inhibition of ecto-ATPase activity enhanced-the survival and prolifera

19 restingly, under nonreducing conditions, the ecto-ATPase activity in rat and pig (unlike chicken and

20 Human ecto-ATPase activity is decreased by NP-40 and at temper

21 consensus sequence completely eliminates the ecto-ATPase activity of CBATP.

22 Despite the intrinsic ecto-ATPase activity of CHO cells, the effective concent

23 onditions, was observed only when endogenous ecto-ATPase activity was pharmacologically inhibited by

24 ry staining, we also demonstrated functional ecto-ATPase activity within the odontoblast layer, subod

25 In addition to the existence of an ecto-ATPase activity, our results suggest a major scaven

26 racellular domain binds eATP and displays an ecto-ATPase activity, thus controlling eATP concentratio

27 amino acid protein (NTPDase2 alpha exhibited ecto-ATPase activity.

28 contain both constitutive and TCDD-inducible ecto-ATPase activity.

29 s actual release as the cells also displayed ecto-ATPase activity.

30 Transgenic upregulation of ecto-ATPase alone confers resistance to organisms that h

31 of either the C- or N-terminus of the human ecto-ATPase alone in the chicken ecto-ATP-diphosphohydro

32 they are promptly degraded into adenosine by ecto-ATPase and 5'-nucleotidase, which have been identif

33 absence of ATP was inhibited by apyrase, an ecto-ATPase and by suramin, an antagonist of P2 receptor

34 The sequence homology between the gizzard ecto-ATPase and CD39 was confirmed by Western blots demo

35 endothelial cell membrane protein with both ecto-ATPase and ecto-ADPase activities.

36 ed the in vivo localization of extracellular ecto-ATPase and ecto-apyrase (ATPDase) in adult chicken

37 Purification and molecular cloning of ecto-ATPase and other canalicular proteins are complicat

38 In contrast to Mrp2, protein expression of ecto-ATPase and P-gp remained unchanged in endotoxin- an

39 clonal antibodies raised against the chicken ecto-ATPase and two commercially available monoclonal an

40 ary structures, the results suggest that the ecto-ATPases and ecto-apyrases are part of, or closely r

41 ST programs demonstrated homology with other ecto-ATPases and ecto-apyrases, including those from the

42 ases have been divided into two subfamilies, ecto-ATPases and ecto-ATP-diphosphohydrolases (ecto-ATPD

43 yrase conserved regions, are present in both ecto-ATPases and soluble E-type ATPases.

44 their ability to cross-react with mammalian ecto-ATPases and were used as specific immunochemical pr

45 f canalicular ecto-adenosine triphosphatase (ecto-ATPase) and mdr P-glycoproteins (P-gp).

46 in that is thought to have bile acid efflux, ecto-ATPase, and cell adhesion properties.

47 ctional protein possessing bile acid efflux, ecto-ATPase, and intercellular aggregating properties.

48 millimolar concentrations of azide, whereas ecto-ATPases appear to lack these two properties.

49 However, the recent cloning of an ecto-ATPase (apyrase) from potato tubers provides a new

50 However, the structural features of this ecto-ATPase are not those anticipated for an in-to-out A

51 degradation: Pannexin1, TRPM5, and NTPDase2 (ecto-ATPase) are indistinguishable between WT and DKO mi

52 ber (Solanum tuberosum) apyrase and parasite ecto-ATPase, are not affected by detergents.

53 that ATP release sites are colocalized with ecto-ATPases at the astrocyte cell surface.

54 y is abolished by prior cross-linking of the ecto-ATPase by lectin and chemical cross-linking agents.

55 ATP is rapidly hydrolyzed by the ecto-ATPase CD39 into adenosine monophosphate (AMP), and

56 The ecto-ATPase CD39 is expressed on exhausted CD8+ T cells

57 sine generation from pericellular ATP is the ecto-ATPase CD39, we next show that inhibition of CD39 a

58 Ecto-ATPase (CD39L1) corresponds to the type 2 enzyme of

59 A full-length E(ecto)-ATPase cDNA was cloned from a human brain cDNA lib

60 rat liver canalicular bile acid transporter/ecto-ATPase/cell CAM 105 (CBATP) is a 110-kDa transmembr

61 rat liver canalicular bile acid transporter/ecto-ATPase/cell CAM 105 (CBATP), a member of the carcin

62 ecent studies have established that dominant ecto-ATPases consist of enzymes now called nucleoside tr

63 , sensitivity to alphabeta-methylene ATP and ecto-ATPase-dependent recovery from endogenous desensiti

64 TM cells expressed ecto-ATPases E-NPP1-3, E-NTPD2, E-NTPD8, and CD73.

65 Human ecto-ATPase (E-NTPDase 2) and chicken ecto-ATP-diphospho

66 converted the eNTPDase-3 ecto-apyrase to an ecto-ATPase (eNTPDase-2), mainly by decreasing the hydro

67 ount for the observed species differences in ecto-ATPase enzymatic properties.

68 lts obtained with the rabbit skeletal muscle ecto-ATPase enzyme, cross-linking the chicken gizzard sm

69 he presence of one or more calcium-dependent ecto-ATPases (enzymes that hydrolyze extracellular 5'-tr

70 Bile acid transport activity and ecto-ATPase expression were analyzed in primary rat hepa

71 This is the second ecto-ATPase family member and the first ecto-ATPDase to

72 iphosphohydrolase (ATPDase), a member of the ecto-ATPase family, was purified to homogeneity previous

73 sed the existence and functional activity of ecto-ATPase for extracellular ATP degradation.

74 se could effectively compete with endogenous ecto-ATPases for released ATP.

75 The ecto-ATPase from chicken gizzard (smooth muscle) was sol

76 Ecto-ATPases from transformed human trabecular meshwork

77 Induction of ecto-ATPase gene expression by TCDD is direct and occurs

78 30 kDa immunoreactive band, proposed to be a ecto-ATPase homodimer, and a concomitant decrease in a a

79 whereas agents and conditions destabilizing ecto-ATPase homooligomers would inhibit the ecto-ATPase.

80 d to be an intermolecularly disulfide-linked ecto-ATPase homotrimer.

81 d RNA analyses showed very low expression of ecto-ATPase in HTC and HTC-R cells compared with hepatoc

82 The presence of ecto-ATPase in taste buds likely reflects the importance

83 e canalicular ecto-adenosine triphosphatase (ecto-ATPase) in adenosine triphosphate (ATP)-dependent b

84 was readily measured even in the absence of ecto-ATPase inhibition suggesting that the spatially col

85 opylxanthine) (10 nm) and was reduced by the ecto-ATPase inhibitor ARL-67156 (6-N,N-diethyl-D-beta,ga

86 use intravesical perfusion of apyrase or the ecto-ATPase inhibitor ARL67156 altered reflex bladder ac

87 entiated by alphabetam-ATP as well as by the ecto-ATPase inhibitor ARL67156 and were depressed in the

88 e receptor antagonist, MRS1754, ARL67156, an ecto-ATPase inhibitor, and A2A receptor siRNA, suggestin

89 wild-type mice of either exogenous ATP or an ecto-ATPase inhibitor, ARL-67156, and by exposure of hep

90 The chicken muscle ecto-ATPase is a slightly basic (predicted pI = 7.93) 49

91 ted by the corresponding region of the human ecto-ATPase is also inhibited by NP-40 and is less activ

92 cto-ATPDase with other reported ATPDases and ecto-ATPases is discussed.

93 The extracellular ATPase (ecto-ATPase) is a divalent cation-dependent nucleoside t

94 The results suggest that the muscle ecto-ATPase may be involved in cell adhesion, since the

95 es that recognize the 66 kDa chicken gizzard ecto-ATPase monomer strengthened the hypothesis that thi

96 pproximately 66 kDa immunoreactive band, the ecto-ATPase monomer.

97 After reduction, ecto-ATPase monomers were found to be approximately 66 k

98 The human ecto-ATPase (NTPDase 2) contains conserved motifs includ

99 s correspond to splice variants of the human ecto-ATPase (NTPDase2 alpha,-2 beta, and -2 gamma).

100 posed that agents and conditions stabilizing ecto-ATPase oligomers stimulate enzyme activity, whereas

101 The presence of ecto-ATPases on ARPE-19 cell membranes was suggested by

102 Cell surface ATPases (ecto-ATPases or E-ATPases) hydrolyze extracellular ATP a

103 D1, and ecto-5'-nucleotidase, but not NTPD2 (ecto-ATPase, or CD39L1), in the rabbit NPE cells.

104 as solubilized, and the 66-kDa cell membrane ecto-ATPase protein was purified.

105 pecific ways to stabilize the native, active ecto-ATPase quaternary structure (the homotrimer).

106 The differences in ecto-ATPase quaternary structure stability may account f

107 The chicken ecto-ATPase showed considerable amino acid sequence homo

108 11b, alpha1, alpha4, and beta1) and CD39 (an ecto-ATPase), suggesting that the in vivo mechanisms of

109 d sequence suggests that the gene encodes an ecto-ATPase that contains multiple glycosylation sites,

110 ytes, of one or more proteins other than the ecto-ATPase that mediate ATP-dependent transport of bile

111 in determining the sensitivity of the human ecto-ATPase to NP-40 and high temperatures; (2) incorpor

112 However, ecto-ATPase was clustered in the sarcolemma of the organ

113 In chicken gizzard, the ecto-ATPase was distributed in discrete clusters restric

114 Ecto-ATPase was immunochemically identified in chicken,

115 the activity and cellular trafficking of the ecto-ATPase were examined.

116 ies, both raised against the chicken gizzard ecto-ATPase, were evaluated for their ability to cross-r

117 re, and substrate resemble that of the human ecto-ATPase, which donates the C-terminus including the

118 ecto-ATPDase is equally distant from the two ecto-ATPases, which exhibit low activity toward ADP, and

119 ss-linking the chicken gizzard smooth muscle ecto-ATPase with 3,3'-dithiobis(sulfosuccinimidylpropion