ライフサイエンスコーパス: editing

1 icircles bearing guide RNAs (gRNAs) for mRNA editing.

2 ntaining minimal Cas9-independent off-target editing.

3 th in meiotic and post-meiotic germ cell RNA editing.

4 ibited normal expression patterns after gene editing.

5 ere modified, with no evidence of off-target editing.

6 otecting pre-mRNA upon KPAF3 displacement by editing.

7 ng including checkpoint inhibitors, and gene editing.

8 he arsenal of enzymes used in precise genome editing.

9 similar to known negative regulators of RNA editing.

10 A-mediated or Csy4-mediated multiplex genome editing.

11 e, constraining the sequences accessible for editing.

12 platform for eukaryotic genome and epigenome editing.

13 enough to be packaged into an AAV for genome editing.

14 ign of guide RNAs for ADAR-mediated RNA base editing.

15 ction, RNA interference and potentially gene editing.

16 ing corrected lines by genome- and epigenome-editing.

17 nal mapping, gene expression, silencing, and editing.

18 e, dogs, and human cells through CRISPR/Cas9 editing.

19 he proper use of CRISPR-Cas9-mediated genome editing.

20 ibition, gene activation and programmed gene editing.

21 AR inhibitors and new tools for directed RNA editing.

22 f the MHC I-chaperone complex and to peptide editing.

23 anonical active sites for aminoacylation and editing.

24 ed pluripotent stem cells by CRISPR/Cas9gene editing.

25 DNA target site during genome and epigenome editing.

26 2) depends on adr-1, a gene important to RNA editing.

27 ns in Arabidopsis At2OGO by CRISPR/Cas9 gene editing.

28 to T*A, in cellular DNA for precision genome editing.

29 ble, drug loadable, and suitable for genetic editing.

30 significantly muffle the single-nucleic-acid editing ability of the dCas13a RNA-editing system.

31 king of edited cells (BAR-Seq) and show that editing activates p53, which substantially shrinks the H

32 shown to have reduced off-target RNA and DNA editing activities(5,6).

33 hes a critical role for ADAR1 and its A-to-I editing activity during cell fate transitions and deline

34 cer (CRC) cells, thus displaying high genome-editing activity in vitro.

35 Creating accurate maps of A-to-I RNA editing activity is vital to improving our understanding

36 Furthermore, loss of Adar RNA editing activity leads to innate immune induction, indic

37 plored many approaches to improve the genome editing activity of the CRISPR-Cas system and deliver it

38 We propose that the cross-editing activity of ThrRS is evolutionarily conserved an

39 te that these ADAR2 variants lead to reduced editing activity on a known ADAR2 substrate.

40 Editing activity was sensitive to addition of zinc aceta

41 ning of Cas9 expression to achieve high gene editing activity without detectable toxicity.

42 eaminase domain which is important for ADAR1 editing activity.

43 rdant results for sites with high off-target editing activity.

44 rt palindromic repeat (CRISPR)-mediated gene editing and acetate supplementation of the culture media

45 and to identify potential targets for genome editing and breeding.

46 unintended consequences including off-target editing and chromosomal translocations.

47 reduced repertoire diversity, decreased BCR editing and developmental arrest of immature B cells, re

48 Here, we have combined CRISPR gene editing and engineered separation-of-function mutants to

49 his version does not include post-acceptance editing and formatting.

50 al dementia patient using CRISPR/Cas9 genome editing and homology-directed repair (HDR), resulting in

51 - and development-specific regulation of RNA editing and identify a molecular mechanism that regulate

52 dvances, such as the establishment of genome editing and improved phylogenetic resolution, are paving

53 ain proteins, ADAD1 and ADAD2, on testis RNA editing and male germ cell differentiation.

54 CRISPR-Cas gene editing and messenger RNA-based protein replacement ther

55 s the recent applications of tRNAs in genome editing and microbiome sequencing.

56 from functional experiments, such as genome editing and reporter assays.

57 ts and a combination of CRISPR-mediated gene editing and RNAi-mediated gene silencing in human cells,

58 Using both locus-specific genetic editing and simultaneous epigenetic silencing of multipl

59 also discuss the importance of NMD for gene editing and tumor evolution, and how inhibiting NMD may

60 h 3' adenylation, uridine insertion/deletion editing, and 3' A/U-tailing.

61 extends dCas9 residence times, delays genome editing, and alters the balance between indel formation

62 s9-dependent and -independent DNA off-target editing, and in transcriptome-wide RNA off-target editin

63 NNGG PAM, displays high activity for genome editing, and is compact enough to be packaged into an AA

64 er-free DNA in rice using CRISPR-Cas9 genome editing, and offer a promising strategy for genetic impr

65 mental immunology, genetic engineering, gene editing, and synthetic biology exponentially expand oppo

66 efSaCas9 can be broadly used in genome-editing applications that require high fidelity.

67 ion of regulatory regions and multiplex base editing applications.

68 system has been widely used for various gene editing applications.

69 We show here, using a gene-editing approach, that a point mutation that eliminates

70 NPs, could not be reached with a single gene-editing approach.

71 This protocol thus expands CRISPR-based gene editing approaches beyond models of robust T cell activa

72 Future trials should use superior gene editing approaches to improve therapeutic efficacy.

73 ew focuses on new state-of-the-art epigenome editing approaches to modify the epigenome of neoplasms

74 Using a combination of gene-editing approaches, quantitative imaging, and biochemica

75 d flexibility, assays to identify off-target editing are becoming more comprehensive and sensitive.

76 icipating and verifying the result of genome editing are essential for the success for all applicatio

77 Advances in gene editing are leading to new medical interventions where p

78 requisites necessary for CRISPR/Cas-mediated editing as well as the current challenges.

79 argets of RNA-binding proteins identified by editing) as an approach to identify signaling pathways t

80 reased in adr-1 mutants due to deficient RNA editing at a single adenosine in their 3'-UTR.

81 discoveries illuminate base editing, enable editing at previously intractable targets, and provide n

82 e, this model is also not amenable to genome-editing based therapeutic approaches.

83 study reports widespread differences in RNA editing between epithelial and mesenchymal tumors and a

84 ing to as high as 50% and improves multiplex editing by 5- to 10-fold in E. coli, while PapRecT enabl

85 robust inhibition of SauCas9-induced genome editing by AcrIIA13 and moderate inhibition by AcrIIA14

86 nucleolytic processing by TREX1 and cytosine editing by APOBEC3B.

87 Genome editing by CRISPR (clustered regularly interspaced short

88 ng, and in transcriptome-wide RNA off-target editing can be ameliorated by the introduction of an add

89 l CRISPR-Cas9 homology-directed repair, base editing can correct point mutations without supplying a

90 Gene editing can rapidly improve a range of crop traits, incl

91 ions, and major efforts have broadened their editing capabilities, expanded their targeting scope and

92 , and provide new base editors with improved editing capabilities.

93 ed deaminase (AID) and apolipoprotein B mRNA editing catalytic polypeptide-like (APOBEC) mutagenesis

94 While editing concludes in the 5' region, KPAF1/2 dimer induce

95 roduction of this minor allele SNP by genome editing confirmed its functionality in depressing GATA6

96 Adenosine (A) to inosine (I) RNA editing contributes to transcript diversity and modulate

97 redited in thyroid tumors, and its levels of editing correlate with a worse progression-free survival

98 Using CRISPR/Cas9-mediated gene editing, coupled with endocrine cell differentiation str

99 editors are useful tools for precise genome editing, current base editors can only convert either ad

100 cyl flux around the phosphatidylcholine acyl editing cycle was the largest acyl flux reaction in wild

101 ucible conditional mice, or CRISPR/Cas9 gene editing decreased cell migration due to the longer durat

102 The oxidized Pol gamma becomes editing-deficient, displaying a 20-fold elevated mutatio

103 rkably, CRISPR/Cas9-guided single-nucleotide editing demonstrated the direct effect of rs7198799 on Z

104 mesenchymal tumors and a novel mechanism of editing-dependent regulation of mRNA abundance.

105 The present CRISPR/Cas9 gene editing dogma for single guide RNA (sgRNA) delivery is b

106 genes, ribosomal protein S12 (RPS12), the 5' editing domain of NADH dehydrogenase subunit 7 (ND7 5'),

107 licing, while the second major mechanism-RNA editing due to post-transcriptional changes of individua

108 rus that significantly improve citrus genome editing efficacy.

109 programmable A*T to G*C point mutations but editing efficiencies can be low at challenging loci in p

110 in cells and allows for titratable levels of editing efficiency and spatial patterning via selective

111 cles with polyglutamic acid further improves editing efficiency by approximately twofold, reduces tox

112 are 46 RNA editing loci with an average RNA editing efficiency of 63%.

113 single gene, the lipoMSN achieved a 54% gene-editing efficiency, besting the state-of-art Lipofectami

114 be optimized, and challenges regarding their editing efficiency, specificity and immunogenicity must

115 , but this comes at the expense of on-target editing efficiency.

116 hat regulates ADAR substrate recognition and editing efficiency.

117 crRNA for Cas12a and obtained a much higher editing efficiency.

118 e for its cognate PAM promotes higher genome-editing efficiency.

119 These discoveries illuminate base editing, enable editing at previously intractable target

120 Prime editing enables diverse genomic alterations to be writte

121 Apolipoprotein B editing enzyme, catalytic polypeptide 3 (APOBEC3) family

122 DYW-deaminase domains act as the editing enzyme.

123 In addition, the genome editing enzymes themselves need to be optimized, and cha

124 Non-canonical and C-to-U RNA-editing events are enriched inside and/or adjacent to MN

125 djacent to MNRs, while all categories of RNA-editing events are under-represented in DNRs.

126 However, elucidation of RNA editing events at transcriptome-wide level requires incr

127 discusses how to anticipate and detect those editing events by a combination of assays to capture all

128 he details of uridine insertion and deletion editing events upon the kDNA transcriptome.

129 scellaneous genomic features, especially RNA editing events.

130 is an important consideration for any genome editing experiment, and a number of Cas9 variants have b

131 SAMMI also offers a wide array of manual map editing features.

132 as) nucleases have revolutionized the genome editing field.

133 ation of MTV in 105 (76%) scans, with simple editing for a satisfactory result in additionally 20% of

134 ++) and HiFi-Sc(++) extend the use of CRISPR editing for diverse applications.

135 -cell RNA-sequencing and CRISPR-Cas9 barcode editing for elucidating developmental lineages at the wh

136 HSPCs as a feasible alternative to nuclease editing for HSC-targeted therapeutic genome modification

137 eptibility target has implications in genome editing for novel plant resistance against devastating H

138 tensive uridine insertion/deletion (U-indel) editing for their maturation.

139 therefore prove beneficial for ex vivo gene editing, for enhanced platelet production, and for the i

140 R33A]) that display comparable DNA on-target editing frequencies, whilst eliciting a 12- to 69-fold r

141 ted later and retained relatively higher RNA editing frequency.

142 RNA editing generates modifications to the RNA sequences, th

143 nCas9 mouse allows robust and tunable genome editing granting flexibility, speed and uniformity at le

144 transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalia

145 mutant jellyfish generated using CRISPR-Cas9 editing had severe defects in gamete development or in s

146 The clinical application of CRISPR-Cas9 gene editing has been eagerly awaited since the first descrip

147 Genome editing has powerful applications in research, healthcar

148 CRISPR/Cas9 genome editing has revolutionized functional genomics in verteb

149 Recent advances in gene editing have been enabled by programmable nucleases such

150 Although recent improvements in genome editing have made it possible to directly modify the tar

151 of the NANOS2 gene generated by CRISPR-Cas9 editing have testes that are germline ablated but otherw

152 recent advances, including CRISPR-based gene editing, have made possible systematic screens for synth

153 Proteomic analysis and CRISPR/Cas9 gene editing identified the inflammatory glycoprotein PTX3 en

154 that short guide RNAs can also support base editing if they contain cytosines within the deaminase a

155 We found that A-to-I editing impedes MDA5 sensing and sequestration of dsRNAs

156 ation in the dimerization interface inhibits editing in an RNA substrate-dependent manner for both AD

157 t implications for the application of genome editing in both basic research and clinical practice.

158 ted miRNA-binding site by CRISPR-Cas9 genome editing in C. elegans We developed a multiplexed negativ

159 icism to levels that make single-step embryo editing in cattle commercially feasible.

160 increased the speed and precision of genetic editing in cells and animals.

161 escribe a platform for efficient Cas12a gene editing in Drosophila We show that Cas12a from Lachnospi

162 lopment and the first to interrogate U-indel editing in EMF and MCF life cycle stages.

163 d variants mediated indel formation and base editing in human cells and enabled A*T-to-G*C base editi

164 of human association analysis, CRISPR genome editing in mice, animal behavioural analysis and cell cu

165 ll as extremely efficient and precise genome editing in P patens Additionally, careful phylogenetic s

166 rmore, our highly efficient CRISPR/Cas9 gene editing in primordial germ cells represents a substantia

167 ere we establish CRISPR/Cas9-mediated genome editing in S. rosetta by engineering a selectable marker

168 stance conferred by precise CRISPR/Cas9 gene editing in the chicken.

169 enario where levels of mature RNA species or editing in the single T. cruzi mitochondrion are linked

170 incorporated zebularine also did not disrupt editing in vitro, suggesting that PPR65 cannot bind modi

171 ogy for memory CD8 T cells to undertake gene editing in vivo, for the first time, to our knowledge.

172 idaminococcus spec., can mediate robust gene editing in vivo.

173 R/Cas9 has become a powerful tool for genome editing in zebrafish that permits the rapid generation o

174 In vitro genome editing indicated that the SRF enhancer CArG box regulat

175 lian bloodstream forms (BSF) at the level of editing initiation and/or edited mRNA stability.

176 CRISPR-Cas9-associated base editing is a promising tool to correct pathogenic single

177 Within the testis, RNA editing is catalyzed by ADARB1 and is regulated in a cel

178 Genome editing is-or will soon be-in the clinic for several dis

179 Impaired ADAR2 editing leads to early-onset epilepsy and premature deat

180 ells that loss of RAZUL by CRISPR-based gene editing leads to loss of E6AP at proteasomes.

181 papaya chloroplast genome, there are 46 RNA editing loci with an average RNA editing efficiency of 6

182 PTEN silencing with CRISPR/dCas9 epigenetic editing may provide a new option for promoting axon rege

183 Due to breakthroughs in RNAi and genome editing methods in the past decade, it is now easier tha

184 Using genome editing methods, we disrupt uridine monophosphate synthe

185 mapping-by-sequencing and CRISPR/Cas9 genome editing methods, we isolated EXCESSIVE NUMBER OF FLORAL

186 h the ability to precisely target by genomic editing most areas of the genome, is producing important

187 ding screens that harness CRISPR/Cas9 genome editing, natural genetic variation, proteomics, and tran

188 apeutics and the improvement of precise gene editing now pave the way to applications such as cardiac

189 vergence including alternative splicing, RNA editing, nuclear pore composition, RNA-binding protein m

190 Gene editing nuclease represented by Cas9 efficiently generat

191 AR substrates and the features governing RNA editing observed in our study will assist in the rationa

192 g in human cells and enabled A*T-to-G*C base editing of a sickle cell anemia mutation using a previou

193 Gene editing of a small deletion in the first coding exon sup

194 RNA libraries, which can be used for genome editing of coding and non-coding genomic regions effecti

195 pression in mouse cells and CRISPR/Cas9 base editing of endogenous AGS loci revealed causal roles of

196 Here, we describe CRISPR/Cas9-based editing of exon 1 of the HVT079 and HVT096 genes from th

197 esults demonstrate the potential of RNP base editing of human HSPCs as a feasible alternative to nucl

198 aced short palindromic repeats (CRISPR)-Cas9 editing of immune checkpoint genes could improve the eff

199 Congruently, gene editing of LAYN in human CD8+ T cells reduced direct tum

200 ion processes has enabled precise, multiplex editing of microbial genomes and the construction of bil

201 Precise gene editing of mitochondrial DNA (mtDNA) is essential for th

202 A-to-I editing of RNA is a widespread posttranscriptional proce

203 ADAR1), responsible for adenosine-to-inosine editing of RNA, is required for regulating the developme

204 few approaches have been developed for gene editing of these cell types, likely owing to their sensi

205 stence of fetal hemoglobin (HPFH) mutations, editing of transcriptional HbF repressors or their bindi

206 injection) to detect off-target mutations by editing one blastomere of two-cell mouse embryos using e

207 te whether a mutation has resulted from gene editing or from traditional breeding techniques; (ii) it

208 e with no signs of clonal deletion, receptor editing, or B cell anergy.

209 typic penetrance due to the mosaic nature of editing outcomes after double strand break (DSB) repair.

210 y efficiencies, and suppression of imprecise editing outcomes at the on-target site as key design par

211 hes can be used to forecast CRISPR/Cas9 gene editing outcomes in Xenopus tropicalis, Xenopus laevis,

212 In this study, we analyzed the editing patterns of three putative dual-coding genes, ri

213 We briefly introduce three epigenome-editing platforms: zinc-finger proteins, transcriptional

214 or genotoxicity was associated with the gene editing process, paving the way for an alternative, yet

215 alternative to the most commonly used genome editing protein Streptococcus pyogenes Cas9 (SpyCas9), w

216 Successful development of IVF and CCR5 editing protocols in MCM embryos lays a foundation for t

217 We were able to achieve an editing rate of > 99% for multiple genes that functional

218 to produce an inconsistent range of genomic editing rates between 0.03-3%.

219 oligodeoxynucleotides mediated precise gene editing rates, and effectively reduces on-target inserti

220 An alternative is to introduce genome editing reagents and a homologous recombination (HR) don

221 Developmental regulators and gene-editing reagents are delivered to somatic cells of whole

222 ng computational algorithms to design genome editing reagents can mitigate off-target edits in plants

223 Here, we introduced genome editing reagents into single-cell bovine embryos to comp

224 d the safety and efficacy concerns of genome editing remain.

225 Efficient base editing requires cellular exposure to levels of base edi

226 ver, traditional CRISPR/Cas9-mediated genome editing requires plant tissue culture that is both time-

227 However, CRISPR-mediated gene editing revealed that PKA and AMPK are not required for

228 CRISPR-mediated gene editing shows promise to cure genetic pathologies, altho

229 uption of GAL5.1 in mice using CRISPR genome editing significantly reduced GAL expression in the amyg

230 esponse, epithelial-mesenchymal differential editing sites are enriched in genes involved in immune a

231 NA-seq) enables global identification of RNA-editing sites in biological systems and disease.

232 salient step in many studies is to identify editing sites that statistically associate with treatmen

233 1) in DLD-1 CRC cells using CRISPR/Cas9 gene editing; some cells were transfected with plasmids that

234 expanded their targeting scope and improved editing specificity.

235 formation to facilitate the design of genome-editing strategies, showcase the past and future transge

236 Here we show that a CRISPR/Cas9-based genome editing strategy allows the precise correction of WAS mu

237 analyze differences between ADAR1 and ADAR2 editing substrates.

238 nosoma brucei possesses a highly complex RNA editing system that uses guide RNAs to direct the insert

239 leic-acid editing ability of the dCas13a RNA-editing system.

240 PR/dCas9-based enhancer-targeting epigenetic editing systems, enCRISPRa and enCRISPRi, for efficient

241 ced short palindromic repeats -based genetic editing systems, immunotherapy, microbiome restoration,

242 domains: vaccine research, viral transcript editing, T-cell effector response targeting including ch

243 -membrane-coating nanotechnology and genetic editing technique offers a safe and robust strategy in a

244 Gene-editing techniques are currently revolutionizing biology

245 Herein, we exploited spectral editing techniques to identify and quantify the lipid mo

246 SORT is compatible with multiple gene editing techniques, including mRNA, Cas9 mRNA/single gui

247 aracterizing new varieties generated by gene-editing techniques.

248 With the rapid development of new genome-editing technologies and the availability of increasing

249 S. viridis research, highly efficient genome editing technologies are needed to create genetic variat

250 uman beta-cell lines, and advances in genome-editing technologies coupled with improved protocols dif

251 emonstrations, and recent advances in genome editing technologies may enable the use of reporters in

252 me sequencing along with development of gene editing technologies.

253 cal step in the future translation of genome editing technologies.

254 The advent of genome editing technology provides new opportunities to correct

255 d BMI1 as the direct target of AR using gene-editing technology.

256 ADR-1 promotes SLO-2 function not by editing the transcripts of slo-2 but those of scyl-1, wh

257 Using CRISPR/Cas9 genome editing, the enhancer cluster or parts thereof, Nppb and

258 ead to the ultimate realization of molecular editing: the freedom to modify organic molecules at any

259 osing organisms, designing robots capable of editing their own structure to more efficiently perform

260 development of efficient, combinatorial gene-editing therapeutics.

261 important in the development of directed RNA editing therapeutics.

262 been subjected to CRISPR/Cas9-mediated gene editing, there is no evidence of success in genetic alte

263 With recent advances in genome editing, this type of genetic mutation can be precisely

264 ecT increases the efficiency of single-locus editing to as high as 50% and improves multiplex editing

265 ene-edited cell populations and expands gene editing to chromosomal tracts previously not possible to

266 Genome editing to correct a defective beta-globin gene or induc

267 This work establishes that precise gene editing to correct multiple distinct gene variants could

268 Using genome editing to delete candidate REs, we showed that a strong

269 We then use genome editing to disrupt the coding sequence of a S. rosetta C

270 Employing CRISPR/Cas9 gene editing to disrupt the Pdgfra gene in two different muri

271 Using CRISPR/Cas9 editing to endogenously tag receptors with fluorescent p

272 Here we use genome editing to engineer a general platform to improve the sa

273 We used genome editing to generate a Gdf15 (nuGFP-CE) mouse line, remov

274 The potential of genome editing to improve the agronomic performance of crops is

275 We previously used gene editing to introduce a dual epitope tag into the endogen

276 cobalamin metabolic enzymes, we used genome editing to study the loss of mmachc function and to deve

277 chnological approaches, mainly based on gene editing, to produce allogeneic CAR T cells with limited

278 describe an optimized Cas9-AAV6-based genome editing tool platform for site-specific mutagenesis and

279 CRISPR/Cas9 is a programmable genome editing tool widely used for biological applications and

280 Thus, REPAIRx markedly expands the RNA editing toolkit and illustrates a novel strategy for bas

281 Here, we have developed multiplex genome editing toolkits for citrus including PEG-mediated proto

282 We have developed multiplex genome editing toolkits for citrus that significantly improve c

283 The development of new CRISPR-Cas genome editing tools continues to drive major advances in the l

284 the applicability of CRISPR-associated gene editing tools in vitro and in vivo.

285 ceosome, with potential applications as gene-editing tools.

286 tial for application as modulators of genome editing tools.

287 an steers a tapasin loop involved in peptide editing toward the binding groove.

288 Genome editing typically involves recombination between donor n

289 rt and promising perspective of in vivo gene editing using non-viral nano-vectors.

290 Genome editing using programmable nucleases is revolutionizing

291 Expressed from the P gene through mRNA editing, W shares a common N-terminus with P and V but h

292 combining quantitative genetics with genome editing, we show how multiple SVs that changed gene dosa

293 With CRISPR-Cas9 genome editing, we validated Less Shattering1 (SvLes1) as a gen

294 levels of successful heritable plant genome editing were addressed using simple case studies in Arab

295 Ajugoideae, and are generally devoid of RNA editing, whereas moderately diverged genes accelerated l

296 ergence of high-throughput assays and genome editing, which are switching the paradigm from bottom-up

297 ominant Best disease iPSC-RPE models to gene editing, which produced premature stop codons specifical

298 y a homology-directed repair template during editing with Cascade-Cas3, but not Cas9.

299 Cas12i could potentially be used for genome editing with high specificity.

300 ation of ribonucleoprotein attenuates genome editing within cells and allows for titratable levels of

301 ) reveal a novel mechanism of stereochemical editing within peptidoglycan transpeptidation, (c) asses