ライフサイエンスコーパス: electron tomography

1 significant progress towards this goal using electron tomography.

2 unprecedented level of 3D information using electron tomography.

3 overall 3D distribution of nanotunnels using electron tomography.

4 sed on the pore space topology determined by electron tomography.

5 opy, nuclear magnetic resonance imaging, and electron tomography.

6 of addressing this major challenge is atomic electron tomography.

7 ls in Xenopus laevis egg extracts using cryo-electron tomography.

8 ion beam milling and then visualized by cryo-electron tomography.

9 f these domains are investigated by means of electron tomography.

10 ze substitution (HPF/FS) in combination with electron tomography.

11 -polarity organization, as confirmed by cryo-electron tomography.

12 nt angles in vitreous ice: cryo-Transmission Electron Tomography.

13 olution transmission electron microscopy and electron tomography.

14 le onto the growing axoneme tip using (cryo) electron tomography.

15 aged using Zernike phase contrast (ZPC) cryo-electron tomography.

16 ve study of CENP-A nucleosome arrays by cryo-electron tomography.

17 nal structure of a native HIV-1 core by cryo-electron tomography.

18 matrix factorization, compressed sensing and electron tomography.

19 cations in materials at atomic resolution by electron tomography.

20 ture of RIM1alpha knockout (KO) mice by cryo-electron tomography.

21 endently by atomic force microscopy and cryo-electron tomography.

22 freeze-substituted cells, as well as by cryo-electron tomography.

23 ts spectrin network from proteomics and cryo-electron tomography.

24 plexes, using X-ray crystallography and cryo-electron tomography.

25 gth primary cilia obtained by serial section electron tomography.

26 Finally, we show by electron tomography [3D transmission electron microscope

27 Three-dimensional imaging using electron tomography allowed us to count the number of ac

28 Cryo-electron tomography allows the imaging of macromolecular

29 Electron tomography analyses indicated that chloroplast

30 Electron tomography analysis indicated that ribosome den

31 tion at the subcellular scale as informed by electron tomography and 2) meshing a protein with a stru

32 Using three-dimensional electron tomography and analysis of spindle dynamical be

33 haracterization of the products by multimode electron tomography and analysis of the NC morphologies

34 In this work, we used electron tomography and confocal microscopy to reconstru

35 lation of low-resolution structure data from electron tomography and cryo-electron microscopy, here w

36 aging membranes by both high-resolution cryo-electron tomography and diffraction-limited optical micr

37 a three-dimensional structure model through electron tomography and direct imaging of surface topogr

38 Electron tomography and genetic analysis indicate that E

39 tochores were examined in diverse species by electron tomography and image analysis.

40 to structural data on spindles obtained from electron tomography and laser ablation.

41 Integrating techniques in electron tomography and molecular virology, we defined a

42 E. coli minicells as a host, along with cryo-electron tomography and mutant phage virions, to visuali

43 Here we utilize electron tomography and other methods to investigate the

44 ned to inpaint the missing-wedge sinogram of electron tomography and reduce the residual artifacts in

45 In this study, we imaged T8I virions by cryo-electron tomography and showed that T8I mutants, like MI

46 Here we use cryo-electron tomography and sub-tomogram averaging to derive

47 ion machine obtained by high-throughput cryo-electron tomography and sub-tomogram averaging.

48 unit at 11.3 A resolution, obtained by cryo-electron tomography and sub-tomogram averaging.

49 We used cryo-electron tomography and subsequent subtomogram averaging

50 We used electron tomography and subtomogram averaging to derive

51 applied cryo-focused ion beam milling, cryo-electron tomography and subtomogram averaging to determi

52 Here we apply cryo-electron tomography and subtomogram averaging to determi

53 We have used cryo-electron tomography and subtomogram averaging to determi

54 Here, we deploy cryo-electron tomography and subtomogram averaging to determi

55 We used the techniques cryo-electron tomography and subtomogram averaging to obtain

56 We use cryo-electron tomography and subtomogram averaging to provide

57 Here, we have used cryo-electron tomography and subtomogram averaging to study i

58 Using cryo-electron tomography and subtomogram averaging, we determ

59 Using cryo-electron tomography and subtomogram averaging, we visual

60 complex branch junction in cells using cryo-electron tomography and subtomogram averaging.

61 ating live cell imaging with high-resolution electron tomography and super-resolution microscopy.

62 Cellular cryo-electron tomography and thin-section EM studies uncovere

63 ptospira by integrating high-resolution cryo-electron tomography and X-ray crystallography.

64 We used cryo-electron tomography and Zernike phase contrast cryo-elec

65 focal imaging of Ca(2+) sparks and dual-tilt electron tomography) and dSTORM imaging of permeabilized

66 tron microscopy (TEM) using serial sections, electron tomography, and focused ion beam scanning elect

67 lar structures, three-dimensional imaging by electron tomography, and improved image-processing metho

68 oss-linking mass spectrometry, cellular cryo-electron tomography, and integrative modeling to determi

69 Using confocal microscopy, electron tomography, and large volume focused ion beam/s

70 fluorescence recovery after photobleaching, electron tomography, and model convolution simulation of

71 , small-angle x-ray scattering, transmission electron tomography, and nanoscale x-ray computed tomogr

72 light and electron microscopy, in situ cryo-electron tomography, and subtomogram analysis, we reveal

73 ualized by cryo-electron microscopy and cryo-electron tomography, and the network of protein domain i

74 yo-electron microscopy and negative-staining electron tomography approaches to image, and three-dimen

75 e studied haloarchaeal virus His1 using cryo-electron tomography as well as biochemical dissociation.

76 we use a combination of live microscopy and electron tomography, as well as computer simulations, to

77 Electron tomography based on tilted projections also exh

78 Using cryo-electron tomography, Bauerlein et al. reveal the fibrill

79 resolution light microscopy of mutants, cryo-electron tomography, bioinformatic predictions and prote

80 Using electron tomography, CaMKII holoenzymes are clearly iden

81 SPH1 PCD phenotype and demonstrate that cryo-electron tomography can be applied to human disease by d

82 as they are imaged and reconstructed by Cryo-Electron Tomography (CET) and returns densities and coor

83 lution fluorescence microscopy and cryogenic electron tomography (CET) are powerful imaging methods f

84 However, the technology advances in cryo-electron tomography (CET) have made methods to identify

85 Cryo-electron tomography (CET) produces three-dimensional ima

86 Here we use cryo-electron tomography (CET) to study the structure of the

87 the vicinity of HIV-1 budding sites by cryo-electron tomography (cET).

88 tion transmission electron microscopy and 3D electron tomography confirm their structure.

89 aments, whose presence was confirmed by cryo-electron tomography, contributes to endocytic internaliz

90 olution, suggesting that individual-particle electron tomography could be an expected approach to stu

91 ize Bacillus subtilis sporulation using cryo-electron tomography coupled with cryo-focused ion beam m

92 Cryo-electron tomography coupled with epitope tagging and gol

93 n-hydrated biological specimens enabled cryo-electron tomography (cryo-ET) analysis in unperturbed ce

94 Cryo-electron tomography (cryo-ET) can give unprecedented ins

95 Cryo-electron tomography (cryo-ET) enables 3D imaging of macr

96 Cryo-electron tomography (cryo-ET) enables the 3D visualizati

97 y using both negative-staining (NS) and cryo-electron tomography (cryo-ET) experimental data.

98 Cryo-electron tomography (cryo-ET) has reached nanoscale reso

99 Cryo-electron tomography (Cryo-ET) is an imaging technique us

100 Cryo-electron tomography (cryo-ET) is emerging as a revolutio

101 nuclei of eukaryotic cells, achieved by cryo-electron tomography (cryo-ET) of re-vitrified cell secti

102 Cryo-electron tomography (cryo-ET) provides 3D visualization

103 sion electron microscopy (TEM) and cryogenic electron tomography (cryo-ET) results indicate that the

104 iated restriction of HIV-1, we utilized cryo-electron tomography (cryo-ET) to directly visualize HIV-

105 Here we used cryo-electron tomography (cryo-ET) to image the interplay bet

106 Here, we used single particle cryo-electron tomography (cryo-ET) to investigate cyanophage-

107 Here we combined recent developments in cryo-electron tomography (cryo-ET) to produce three-dimension

108 In this study, cryo-electron tomography (cryo-ET) was used to compare pathog

109 f HA-mediated membrane remodeling, here cryo-electron tomography (cryo-ET) was used to image the thre

110 complete fusion products observed using cryo-electron tomography (cryo-ET).

111 individual DNA minicircles observed by cryo-electron tomography (cryo-ET).

112 th high-resolution structural data from cryo-electron tomography (cryo-ET).

113 lied to human adipocytes, and imaged by cryo-electron tomography (cryo-ET).

114 Cryo-electron tomography (cryoET) has become a powerful techn

115 mediate steps can only be resolved with cryo-electron tomography (cryoET).

116 reconstructed from cryoscanning transmission electron tomography (CSTET), namely a dramatic in-plane

117 alian cells using cryo-scanning transmission electron tomography (CSTET).

118 In addition, electron tomography data from intact heart illustrate th

119 ion trajectory with the primary experimental electron tomography data identified regions were snapsho

120 reconstruct 3D volumes at the nanoscale from electron tomography datasets of inorganic materials, bas

121 Electron tomography demonstrated three modes of HIV-1 di

122 Finally, electron tomography demonstrates that the ends of microt

123 perforation, combined with video microscopy, electron tomography, electron energy loss spectroscopy,

124 e dimensions, to produce scanning precession electron tomography, enables the 3D orientation of nanos

125 Cryo-EM data generated by electron tomography (ET) contains images for individual

126 Electron tomography (ET) is a technique that retrieves 3

127 Electron tomography (ET) is a widely used technology for

128 We performed electron tomography (ET) of immature and mature Ty3 part

129 Transmission electron microscopy and 3D electron tomography (ET) of the egress zones revealed cl

130 Electron tomography (ET) provides a tool for imaging a s

131 Electron tomography (ET) studies revealed continuity of

132 tissue clearing, 3D-immunofluorescence, and electron tomography (ET) to longitudinally assess early

133 Here, we utilized advances in electron tomography (ET) to study the antibody flexibili

134 We generated images of CVCs in 3-D using electron tomography (ET), and used immuno-ET to show PHI

135 ural comparison using three-dimensional (3D) electron tomography (ET), determined that desmotubule st

136 ubilized complex is consistent with previous electron tomography experiments and suggests that monome

137 t into the nature of priming, we searched by electron tomography for structural relationships correla

138 g weak-beam dark-field TEM and scanning TEM, electron tomography has been used to image 3D dislocatio

139 of three-dimensional reconstruction of cryo-electron tomography has greatly improved.

140 embranes by atomic force microscopy and cryo-electron tomography has revealed that the visual pigment

141 s, cross-linking mass spectrometry, and cryo-electron tomography, has facilitated the determination o

142 y experiments supported by electron and cryo-electron tomography have provided fresh insights into Ch

143 Imaging nine additional species with cryo-electron tomography, here, we show that this subcomplex

144 Electron tomography, high angle annular dark field scann

145 in clinical medicine through to advancing 3D electron tomography images of nanoparticle catalysts and

146 ctures with complex geometry identified from electron tomography images.

147 By cryo-electron tomography imaging and functional assays, we ob

148 Recent advancements in electron tomography imaging have enabled researchers to

149 Positron electron tomography imaging using radiolabeled antibody

150 associated LRRK2 built using a reported cryo-electron tomography in situ structure(5).

151 We used axial (bright-field) electron tomography in the scanning transmission electro

152 itative transmission electron microscopy and electron tomography in vivo were used to study MDV produ

153 and distribution of such domains analyzed by electron tomography indicate that this is a common pheno

154 m in 2D electron microscopy and 3.3 nm in 3D electron tomography indicates a genuine signalling micro

155 Through individual-particle electron tomography (IPET) 3D reconstruction from negati

156 Individual-particle electron tomography (IPET) is an approach for obtaining

157 ere, we use our reported individual-particle electron tomography (IPET) method with optimized negativ

158 Dual-axis electron tomography is an important 3 D macro-molecular

159 Cryo-electron tomography is an important and powerful techniq

160 Cryo-electron tomography is an important tool to study struct

161 Electron tomography is an increasingly powerful method t

162 As x-ray and electron tomography is pushed further into the nanoscale

163 -dimensional (3D) structure of thin samples, electron tomography is the method of choice, with cubic-

164 Using quantitative light microscopy, electron tomography, laser-mediated ablation, and geneti

165 oach combining atomic force microscopy, cryo-electron tomography, network analysis, and molecular dyn

166 information from 3D electron diffraction and electron tomography of a single nanocrystal, X-ray powde

167 us in cryo-preserved infected cells, by cryo-electron tomography of cellular lamellae.

168 Using cryo-electron tomography of Ebola virus-like particles, we de

169 Cryo-electron tomography of Ede1-containing condensates, at t

170 ons of dividing yeast cells were analyzed by electron tomography of freeze-substituted cells, as well

171 By cryo-electron tomography of frozen-hydrated endothelial cells

172 Electron tomography of high-pressure frozen synapses rev

173 We used cryo-electron tomography of intact Myxococcus xanthus cells t

174 Cryo-electron tomography of liposomes with bound MACA showed

175 Cryo-electron tomography of mia mutant axonemes revealed that

176 Electron tomography of negatively stained Afp revealed r

177 Cryo-electron tomography of phage 'infecting' outer membrane

178 Here we used electron tomography of rigor muscle swollen by low ionic

179 patient) cilia at high-resolution using cryo-electron tomography of samples obtained noninvasively by

180 Cryo-electron tomography of the vitrified sections revealed t

181 We have used electron tomography of well-preserved metaphase cells to

182 Here we combine the first large-scale serial electron tomography of whole mitotic spindles in early C

183 Three-dimensional transmission electron tomography on four KC corneas showed the degene

184 Using electron tomography on mouse trachea, we show that basal

185 performed cryo-electron microscopy and cryo-electron tomography on the self-same specimens.

186 Electron tomography on tissue sections from fixed and st

187 netic resonance imaging [fMRI], and positron electron tomography [PET]) to explain the functional eff

188 Three-dimensional (3D) reconstructions from electron tomography provide important morphological, com

189 Cryogenic electron tomography provides complementary nanometer sca

190 A study was made by a combination of 3D electron tomography reconstruction methods and N2 adsorp

191 By individual-particle electron tomography reconstruction, we obtain 14 density

192 Dsg2 structure has an excellent fit with the electron tomography reconstructions of human desmosomes.

193 se were obtained from the inspection of cryo-electron tomography reconstructions of individual human

194 Cryo-electron tomography resolved the acuna inner structure a

195 Electron tomography retrieves three-dimensional structur

196 Electron tomography revealed HIV-1 virions bound to TZM-

197 Electron tomography revealed that immunogold particles s

198 Electron tomography revealed that the binding of Sec23 i

199 Cryo-electron tomography revealed that the drc3 mutant lacks

200 Electron tomography revealed that these structures consi

201 elative image analyses and three-dimensional electron tomography revealed that this symbiont resides

202 Electron tomography reveals preferential association of

203 In poc1 cells, electron tomography reveals subtle defects in the organi

204 Cryo-electron tomography reveals that microtubules assembled

205 Electron tomography reveals that the nanocrystals have a

206 Electron tomography reveals that the trafficking defects

207 ional organization of these "nano-machines." Electron tomography reveals the internal structure of sy

208 Complementary live-cell imaging and electron tomography show that beta-CTT is necessary to p

209 3D electron tomography showed that cortical actin bundled b

210 Data from fluid-phase HRP electron tomography showed that fibricarriers could orig

211 Cryo-electron tomography showed that in the absence of FAP206

212 Cryo-electron tomography shows ordered binding of part of the

213 microscopy (cryoEM) and single particle cryo-electron tomography (SPT) we characterize the growth of

214 e fully automatic data process for dual-axis electron tomography still remains a challenge due to thr

215 Here we report cryo electron tomography structures of the 96-nm repeat from

216 Genetics and cryo-electron tomography studies reveal that deletion of BB02

217 Second, immunofluorescence and cryo-electron tomography studies suggested that both CheW(1)

218 The XEDS electron tomography study presented here can be generall

219 Confocal microscopy and electron tomography suggest that emerging LDs are nuclea

220 from its cell envelope when examined by cryo-electron tomography, suggesting that SecA2 is important

221 Using different electron tomography techniques, we found that the grana

222 and subsequent transfer to cryo-Transmission Electron Tomography, the resulting tomograms have excell

223 Here, using cryo-electron tomography, TIRF microscopy, and cell membrane-

224 o outline further research needed for atomic electron tomography to address long-standing unresolved

225 Here, we have used cryo-electron tomography to analyze HTLV-1 particle morpholog

226 We used cryo-electron tomography to capture T7 virions at successive

227 We also used cryo-electron tomography to characterize HIV-1 particles prod

228 biochemical and genetic approaches with cryo-electron tomography to compare the CA of wild-type Chlam

229 Here, we use cryo-electron tomography to demonstrate that the microtubule

230 We used light microscopy and electron tomography to elucidate mechanisms of HIV-1 dis

231 e, we have used electron microscopy and cryo electron tomography to elucidate the structural basis of

232 We used electron tomography to examine FcRn transport of Nanogol

233 pulations at postsynaptic sites, we utilized electron tomography to examine GABAergic synapses in dis

234 Our work demonstrates the power of cryo-electron tomography to generate models of previously uns

235 By leveraging in situ cryo-electron tomography to image the native cellular environ

236 Leveraging in situ cryo-electron tomography to image the native molecular landsc

237 In this study, we used electron tomography to investigate stress-induced ultras

238 combined light microscopy and serial-section electron tomography to measure the amount of dimeric and

239 neutron scattering, electron microscopy, and electron tomography to measure the structure of ovalbumi

240 ith cross-linking mass spectrometry and cryo-electron tomography to obtain a composite structure of t

241 Here we use cryo-electron tomography to obtain a detailed view of the org

242 Here, we used cryo-electron tomography to obtain three-dimensional images t

243 We used dual-tilt electron tomography to produce en-face views of dyads, e

244 We used cryo-electron tomography to reveal many previously unrecogniz

245 Previously, we used in situ cryo-electron tomography to reveal the native architecture of

246 Here, we use cryo-electron tomography to show that ER-PM tethers are key d

247 Here we use immuno-electron microscopy and electron tomography to show that rhodopsin is transporte

248 Here, we use cryo-electron tomography to show that, like MIs, the T8I muta

249 use it combines the ability of the classical electron tomography to solve 3D structures and the chemi

250 Here we use atomic electron tomography to study early-stage nucleation in f

251 Here, we used cryo-electron tomography to study the 3D architecture of E-Sy

252 We use cryo-electron tomography to study the infection initiation of

253 roteins, cryofixation, and three-dimensional electron tomography to study the mechanism of synaptic v

254 We used electron tomography to study the presynaptic density in

255 We used cryo-electron tomography to study virion/receptor/liposome co

256 We used cryo-electron tomography to visualize HIV-1 tethered to human

257 We have used cryo-electron tomography to visualize influenza A virus at pH

258 n tomography and Zernike phase contrast cryo-electron tomography to visualize populations of purified

259 We used cryo-electron tomography to visualize the 3D structure of the

260 We used cryo-electron tomography to visualize the binding of CD4 and

261 Here we employed cryo-electron tomography to visualize the intact flagellar mo

262 Electron tomography unexpectedly revealed spermatocyte a

263 In fixed ventricular myocardium, dual-axis electron tomography was used for three-dimensional recon

264 Using electron tomography we have resolved the three-dimension

265 Finally, using electron tomography we reveal that mammalian distal appe

266 Using cryo-electron tomography we show that PACRG and FAP20 form th

267 By Volta phase plate cryo-electron tomography we show that VP5 is monomeric on the

268 ng of vitreous Chlamydomonas cells with cryo-electron tomography, we acquired three-dimensional struc

269 Using electron tomography, we carried out a high-resolution an

270 Using in situ cryo-electron tomography, we confirmed this localization by d

271 genetics, cell biology, proteomics and cryo-electron tomography, we demonstrate that the PilY1 prote

272 Using electron tomography, we demonstrate that this sheet arch

273 Using electron tomography, we explore the 3-dimensional nanost

274 Using cryo-electron tomography, we extended these findings by chara

275 Using fluorescence microscopy and electron tomography, we find that centrioles degenerate

276 combining yeast genetics, biochemistry, and electron tomography, we find that ESCRT-III assembly on

277 Here, using cryo-electron tomography, we have compared the structure of C

278 Using electron tomography, we have examined the 3-dimensional

279 Here, using cryo-electron tomography, we identified virus particles that

280 orrelative light and electron microscopy and electron tomography, we investigated WPB biogenesis in t

281 Using electron tomography, we probed the effects of the heat s

282 Using cryo-electron tomography, we provide structural information o

283 Using electron tomography, we quantitatively defined the uniqu

284 Using cryo-electron tomography, we show that clustered protocadheri

285 nce microscopy, electron microscopy and cryo-electron tomography, we show that dynamin bundles actin

286 Using real-time quantitative imaging and electron tomography, we show that formation of mLDs in c

287 ing real-time imaging and chemical-sensitive electron tomography, we show that it is possible to char

288 mitosis(5)), genetics, live-cell imaging and electron tomography, we show that nuclear fission is ach

289 Using fluorescence microscopy and cryo-electron tomography, we showed that Pseudomonas chlorora

290 Using cryo-electron tomography, we visualize the near-native three-

291 Cryopreservation combined with electron tomography were used to investigate the role of

292 studied at low pH by Volta phase plate cryo-electron tomography, which improves the signal-to-noise

293 th each of the five VMAP deletion mutants by electron tomography, which is necessary to validate memb

294 hin the spherules were also reconstructed by electron tomography, which showed diverse structures.

295 al atoms and a point defect in a material by electron tomography with a precision of approximately 19

296 Here we combine cryo-electron tomography with mass spectrometry, biochemical

297 t for fully automatic alignment of dual-axis electron tomography, with a simultaneous reconstruction

298 spectroscopy, cryo-electron microscopy, cryo-electron tomography, X-ray crystallography, computation,

299 ical measurements on the microscale, whereas electron tomography, x-rays, and NMR have provided insig

300 ly hydrated, vitrified biological samples by electron tomography yields structural information about