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1 lectroporate, but CEM cells were efficiently electroporated.
2 fraction of the cell's surface area that is electroporated.
3 alculate the approximate current required to electroporate a cell with an electrode of a particular s
7 ette filled with DNA or other macromolecules electroporates a single cell at the tip of the micropipe
11 efficiently captured, in the current work we electroporated an I-SceI expression plasmid along with H
12 , where small sample volumes are top-loaded, electroporated and bottom-ejected into 96-well plates.
17 Newly fertilized dwarf surfclam eggs were electroporated at 700 V in the presence of 1 x 10(4) col
20 ing indicates that in osmotically shocked or electroporated bacteria, proteins are strained through a
22 his study develops a model for a single cell electroporated by an external electric field and uses it
23 previously developed model of a single cell electroporated by an external electric field by explicit
25 transiently active anti-CD123 messenger RNA-electroporated CART (RNA-CART123); (2) T-cell ablation w
26 as9 screen of the HBG1 and HBG2 promoters by electroporating CD34+ cells obtained from healthy donors
28 ylene oxide)-poly(ethylene oxide) helps seal electroporated cell membranes, arresting the leakage of
31 sp2 and SARSDeltansp2 viruses recovered from electroporated cells had 0.5 to 1 log10 reductions in pe
36 SL-O permeabilized cells when compared with electroporated cells, suggesting that, after SL-O permea
48 ile viral RNA transcripts were produced from electroporated expression vectors, as determined by reve
50 ase studies of exocytosis, including a large electroporated granule of the mast cell, a small and cle
51 donors assessed in technical triplicates, we electroporated high-fidelity Cas9 protein targeted to th
52 erase (CAT) or luciferase, was detected when electroporating in the presence of 50 microg closed-circ
53 ed animal growth occurred following a single electroporated injection of a mixture of two plasmids (1
54 ere cloned into the shuttle vector pLS88 and electroporated into 4074Deltacps1N to produce 4074Deltac
56 e cloned STb gene (in the plasmid pRAS1) was electroporated into a nonenterotoxigenic strain (226M) w
61 ially produced nanobody-RING fusion proteins electroporated into cells induce degradation of target w
62 hmania RNA virus 1-4 (LRV1-4) sequences were electroporated into cells of Leishmania strain M4147, a
64 alysis, ligated into pre-prepared vector and electroporated into competent Escherichia coli cells.
65 he cpe open reading frame of NCTC 8239, were electroporated into cpe-negative C. perfringens type A,
67 f 222 resultant fusion genes were separately electroporated into fertilized eggs, and their regulator
69 A plasmid containing this construction was electroporated into H. ducreyi 35000HP, and an isogenic
70 tance cassette (aphA) and this construct was electroporated into H. pylori ATCC 43504; allelic exchan
73 cleic acids with very high affinity, were co-electroporated into immortal human cells along with a se
74 ed upstream of inhA in a plasmid vector, was electroporated into M. tuberculosis, and the resulting c
75 z2 promoter-driven luciferase reporter genes electroporated into maize protoplasts together with R an
78 coding region were synthesized in vitro and electroporated into protoplasts that were cultured at 40
79 MB102 DNA, and the adducted viral genome was electroporated into repair-proficient and repair-deficie
81 nd RING-H2 domain truncated rapsyn-EGFP were electroporated into sternomastoid muscles, a strong raps
83 e P210bcrabl32D cell line, we transiently co-electroporated into the growth factor dependent 32D cell
84 otactic in vivo, dominant negative FGFR1 was electroporated into the premigratory cardiac neural cres
85 isolated from these clones, and TCR RNA was electroporated into the same non-MART-1-reactive allogen
86 ology to the integrated 3' portion, was then electroporated into the target cell lines, and selection
88 M13MB102 DNA, and the adducted genomes were electroporated into wild-type or mutS-deficient Escheric
90 on was significantly impaired in Dok-7-siRNA-electroporated legs compared with the contralateral cont
92 lies functions that protect and recombine an electroporated linear DNA substrate in the bacterial cel
95 ort, we have developed a simple technique to electroporate mammalian cells with high throughput on a
96 selectively adsorbs into damaged portions of electroporated membranes, thereby localizing its effect.
100 expression of 5HT6 and SSTR3 was achieved by electroporating mouse embryo cortex in utero with vector
101 hesis and direct measurement of radiolabeled electroporated mRNA indicate that the primary effect of
103 junction (approximately 25%) in Dok-7-siRNA-electroporated muscles, compared with contralateral cont
104 oth spiny and aspiny neurons surrounding the electroporated neuron in each case, in addition to intri
107 , assessing axon complexity and outgrowth of electroporated neurons, comparing wild-type and Ddx17 kn
108 y microarray characterization of FACS-sorted electroporated neurons, were significantly enriched in p
110 The slh1Delta ski2Delta strains translate electroporated nonpoly(A) mRNA with the same kinetics as
111 of GABA(A)-induced calcium responses in most electroporated NPCs led to a 70% decrease in the number
112 e engineering in which Redbeta can anneal an electroporated oligonucleotide to a complementary target
113 nduce formation of pancreatic tumor foci, we electroporated oncogenic plasmids into pancreata of LSL-
114 Using a novel system for microinjecting and electroporating plasmid expression constructs into murin
116 nditional regulation of gene expression from electroporated plasmids in the postnatal rat retina and
119 tripped porcine stratum corneum following an electroporating pulse were studied by time-resolved free
121 recombinant Mycobacterium bovis BCG (rBCG), electroporated recombinant DNA (rDNA) along with an inte
128 application of short, high-voltage pulses to electroporate skin has recently been shown to make it re
129 requent genetic alterations of human ICC, we electroporated Sleeping Beauty-based oncogenic transposo
133 r cell line, large cells should be easier to electroporate than small ones: the minimum electric fiel
134 mV) rectangular voltage pulses were used to electroporate the bilayers, followed by a prolonged, low
135 scribe RNA from the genome-length cDNA, (iv) electroporate the genome-length RNA into cells to recove
136 t the end of the first recording session, we electroporated the neuron with a DNA plasmid to drive GF
139 membrane of all cells within the sample are electroporated, the presented micromotor can perform, vi
140 s protocol involves isolating mouse T cells, electroporating them with a ribonucleoprotein complex an
141 lasts with integrating lentiviral vectors or electroporating them with CRISPR/Cas9 expressing plasmid
142 (17328) or PS/PD/ME- (17359) IL-2 oligo was electroporated to mouse T cell lymphoma cells (TIB 155)
143 Isolated rat left ventricular myocytes were electroporated using single monophasic EP of different d
151 18 +/- 81 RGCs/mm(2) were present in retinas electroporated with alphaA and alphaB expression constru
152 ctivity was significantly inhibited in VSMCs electroporated with anti-Gbeta antibody (56 +/- 5%) and
155 and protoplasts from each tobacco line were electroporated with both DNA encoding the nuclease and d
156 inducible GFP EV reporter (TIGER) mouse was electroporated with CAG-CRE plasmids or crossed with tam
157 sors of these cortical neurons were in utero electroporated with CBSH3- or CBSH3+ DNAs, migrated to t
159 organisms, embryonic chick spinal cords were electroporated with either construct, allowing the frequ
165 es on the acquisition of NMR data from cells electroporated with preformed DNA-ligand complexes.
167 ate olfactory bulb granule cell neurons were electroporated with SLC7A5 or Slc7a5 short hairpin RNA e
168 n resistance marker, and this strain is then electroporated with suicide plasmids containing mariner
169 ogous monocyte-derived dendritic cells (DCs) electroporated with synthetic mRNA (TriMixDC-MEL) are im
171 DSBs occurring in as many as 1 of 100 cells electroporated with the endonuclease expression vector.
176 iously transfected with the SV40 vector were electroporated with the TD-TFOs, frequencies of base pai
177 C. psittaci 6BC infectious particles were electroporated with various concentrations of circular o