ライフサイエンスコーパス: electroporation

1 her physical gene delivery approaches (e.g., electroporation).

2 ing negative dielectrophoresis (nDEP) and AC electroporation.

3 rve can be accomplished by 12-ns PEF without electroporation.

4 n tumor models and normal tissues to calcium electroporation.

5 healthy volunteers who received ADVAX DNA by electroporation.

6 ateral potential field and thus facilitating electroporation.

7 irus-like replicon particles (VRP) or direct electroporation.

8 mperature-dependent process that facilitates electroporation.

9 ric field to magnitudes sufficient to induce electroporation.

10 nificantly smaller threshold field to induce electroporation.

11 viding neuronal progenitors through in utero electroporation.

12 ing based on nonthermal partial irreversible electroporation.

13 mosaic mice were generated through in utero electroporation.

14 lis anterior muscle of adult rats by in vivo electroporation.

15 ld threshold required for inducing bacterial electroporation.

16 nt cells utilizing mechanical penetration or electroporation.

17 the increase in fluorescence intensity after electroporation.

18 n mammalian cells that had been subjected to electroporation.

19 as delivery by needle injection followed by electroporation.

20 erve and in spinal nerve axons after in vivo electroporation.

21 siRNA was then encapsulated into the EMs by electroporation.

22 ic delivery, agitation with glass beads, and electroporation.

23 ents including in vivo electrophysiology and electroporation.

24 permeability of cells, a phenomenon known as electroporation.

25 on, radiofrequency ablation and irreversible electroporation.

26 ul shRNA transfection inside embryos through electroporation.

27 uent reuptake confirming cell viability post electroporation.

28 ecular payloads into chytrid zoospores using electroporation.

29 cytes/fibroblasts co-culturing technique and electroporation.

30 pic pores in cell membranes-a process called electroporation.

31 DEP-based trapping and another DC source for electroporation.

32 r joule heating at electrodes compared to DC electroporation.

33 ed immediately by co-localised intramuscular electroporation.

34 hout the need for transfection agents and/or electroporation, allowing cell-tracking by MRI in both p

35 ) into L2/3 pyramidal neurons using in utero electroporation also results in a hyperexcitable phenoty

36 endogenous environment, using mouse in utero electroporation and a conditional genetic strategy to mi

37 ed delivery of zinc finger nuclease mRNA via electroporation and adeno-associated virus (AAV) 6 deliv

38 ble of continuous, as opposed to batch-wise, electroporation and cell analysis.

39 ning challenges to our full understanding of electroporation and electropermeabilization.

40 need for terminological distinction between electroporation and electropermeabilization.

41 ls, mammalian antibiotic selection, a second electroporation and gene network activation.

42 es, into the mouse retina by ex vivo plasmid electroporation and into the mouse cerebral cortex by in

43 Compared with bulk electroporation and other exosome-production strategies,

44 sely controls the operation (trap, trap with electroporation and release).

45 Neonatal electroporation and shRNA mediated knockdown of Rab27a i

46 orods (AuNRs) were first loaded into PLTs by electroporation and the resulting AuNR-loaded PLTs (PLT-

47 siently express HBV-specific TCRs using mRNA electroporation and to assess their antiviral and pathog

48 echnique called iTRAP that combined in utero electroporation and translating ribosome affinity purifi

49 rio requires the loss of membrane integrity (electroporation) and resulting depolarization as an inte

50 inations were delivered biweekly via in vivo electroporation, and both humoral and CD8 T cell respons

51 ffected cells by reversible and irreversible electroporation, and quantified the uptaken amount of na

52 pandable lattice-tip radiofrequency ablation/electroporation, and ultra-low temperature cryoablation.

53 An electroporation apparatus hyphenated with stopped-flow s

54 The electroporation approach also has the potential to be tr

55 The implementation of this new electroporation approach may benefit many biology studie

56 Moreover, an in utero electroporation approach showed that PNH-related mutants

57 We evaluated three electroporation approaches: (1) a square-wave electropor

58 As MAE works like many single cell electroporation are carried out in parallel, the electro

59 , the underlying mechanisms of cell membrane electroporation are not sufficiently elucidated, in part

60 genous materials, electrical methods such as electroporation, are preferred over chemical and viral d

61 Here, we surveyed batch electroporation as a delivery tool for single polypeptid

62 s success might promote the wide adoption of electroporation as a safe and effective non-viral gene d

63 delivery of CRISPR/Cas9 machinery via zygote electroporation as an alternative to the conventional de

64 nated with a DNA-based vaccine using in vivo electroporation-assisted delivery.

65 NA delivered intramuscularly by Biojector or electroporation at baseline and week 4 followed by intra

66 in mouse embryos of either sex via in utero electroporation at low, intermediate, and high concentra

67 lectroporation (MAE) platform to advance the electroporation-based delivery of DNA and RNA probes int

68 Here, we describe a simple and economic electroporation-based strategy to deliver Cas9/sgRNA rib

69 Additional electroporation-based technologies that may reach clinic

70 Here, we ask if the electroporation behavior of a reduced cell membrane is c

71 vector concentration, cell type/density, and electroporation buffer properties.

72 change of pH by modifying the composition of electroporation buffer.

73 -fold in tumor and skin tissue after calcium electroporation but decreased in skin tissue 4 hours aft

74 ve of the hydrophilic toroidal pore model of electroporation, but also reveal additional complexity i

75 Electroporation by nanosecond electric pulses (nsEP) is

76 Overall, our results suggest that calcium electroporation can elicit a rapid and selective necrosi

77 romising vaccine platform that together with electroporation can elicit significant systemic Ab respo

78 itu transgenesis methods such as viruses and electroporation can rapidly create somatic transgenic mi

79 Here, we demonstrate that on-line electroporation can serve as a method for membrane perme

80 ll analyses but do not provide for effective electroporation capabilities due to the planar arrangeme

81 d by nanostraw treatment, while conventional electroporation changed the expression of more than 2,00

82 s by purified transposase can be achieved by electroporation, chemical transfection or Lipofection of

83 ic postnatal knockdown of DISC1 via in utero electroporation combined with an inducible knockdown exp

84 Furthermore, electroporation, compared to microinjection, results in

85 across multiple gene loci and conclude that electroporation compares very favourably with convention

86 n exposing them to electric fields mimicking electroporation conditions.

87 dback, the structure is suitable for precise electroporation control in single-cells.

88 tion and we show that commercially available electroporation cuvettes of 1 mm gap size reveal higher

89 In utero, electroporation demonstrates that activation of the Nrp2

90 Here we present a flow-through cell electroporation device integrating large-sized flow tube

91 ee injection system (Biojector) or CELLECTRA electroporation device.

92 nts required to porate the membrane, current electroporation devices deliver voltage pulses in the kV

93 igh voltage, while the emerging microfluidic electroporation devices is still limited by their low ce

94 AdC7), Vaccinia virus (VV), and DNA given by electroporation (DNA/EP), all expressing Simian immunode

95 Unlike alternative ablative therapies, electroporation does not affect the structural integrity

96 ntegrated and self-powered system for active electroporation drug delivery shows great prospect for s

97 Using in utero electroporation during corticogenesis, we show that incr

98 gap, we used calcium imaging and single-cell electroporation during oculomotor behaviors to map VPNI

99 In all tumor types tested in vivo, calcium electroporation effectively induced necrosis, with a ran

100 The present study compared electroporation efficiency of bipolar and unipolar nanos

101 Use of AC fields for electroporation eliminates the unwanted side effects of

102 thetic consensus DNA HIV envelope vaccine by electroporation (EP) are better able to maintain durable

103 DNA vaccines delivered with electroporation (EP) have shown promising results in pre

104 Reversible electroporation (EP) using high intensity electric field

105 e response to IM injection administered with electroporation (EP).

106 Mouse in utero electroporation experiments reveal that Nckap1 loss of f

107 nalysis, cell migration assays, and in utero electroporation experiments to probe the importance of t

108 s an electric pulse-generating nano-probe in electroporation experiments with a potential application

109 Determining the critical electric field for electroporation facilitates the development of electropo

110 enesis efficiency when delivering CRISPR via electroporation for the generation of simple knock-in al

111 aring intradermal administration followed by electroporation generated strong immunogenicity and was

112 h more immediate, but transient, pain in the electroporation group.

113 Electroporation has been a widely established method for

114 Electroporation has emerged as a preferred method for de

115 delivery methods for nucleic acids, such as electroporation, have advanced the field, but have negat

116 c protocol screening process in current bulk electroporation (i.e., electroporation to a large popula

117 Twenty-one dogs underwent injection+electroporation in the posterior left atrium of plasmid

118 PRG-2 electroporation in the PRG-2(-/-) thalamus restored the

119 tion of the biphasic pulse conditions during electroporation increased the survival rate.

120 were designed to unambiguously separate the electroporation-induced sensitization and desensitizatio

121 Conventional electroporation infrastructure is typically immobile, no

122 y elevated Notch signaling, induced by N1ICD electroporation, inhibited gliogenesis in wildtype anima

123 full-genome cDNA was highly infectious after electroporation into cells, producing 2.9 x 10(6) plaque

124 After electroporation into human cells, recombinant fluorescen

125 address these issues, we performed in utero electroporation into the developing rat brain to interfe

126 At 2 days after in utero electroporation into the ventricle of the developing bra

127 s) electric fields, suggesting that cellular electroporation involves additional structures and proce

128 logy to treat solid tumors with irreversible electroporation (IRE) and electrochemotherapy (ECT).

129 tion in bone marrow cells after irreversible electroporation (IRE) and to evaluate the antitumoral ef

130 Irreversible electroporation (IRE) as a non-thermal tumor ablation te

131 Irreversible electroporation (IRE) as newer ablation modality has bee

132 gate the safety of percutaneous irreversible electroporation (IRE) for locally advanced pancreatic ca

133 focal ablative technique called irreversible electroporation (IRE) has been shown to modulate this en

134 d electroporative ablation with irreversible electroporation (IRE) in appropriately selected animal m

135 gate the efficacy and safety of irreversible electroporation (IRE) in the treatment of hepatic tumors

136 sess the safety and efficacy of irreversible electroporation (IRE) in the treatment of patients with

137 Background Irreversible electroporation (IRE) is a nonthermal ablative method ba

138 Irreversible electroporation (IRE) is a promising new nonthermal abla

139 Irreversible electroporation (IRE) is a promising non-thermal treatme

140 Irreversible electroporation (IRE) is an emerging focal therapy which

141 Irreversible electroporation (IRE) is an energy delivery system, effe

142 ose To determine the effects of irreversible electroporation (IRE) on the neural tissues after ablati

143 ve modulation of that stroma by irreversible electroporation (IRE), a local ablation technique that h

144 Irreversible electroporation (IRE), a nonthermal ablative technique,

145 adiofrequency (RF) ablation and irreversible electroporation (IRE), Bulvik et al demonstrated substan

146 lls treated with high-frequency irreversible electroporation (IRE).

147 Electroporation is a basic yet powerful method for deliv

148 Electroporation is a biophysical phenomenon that has sho

149 though transport of molecules into cells via electroporation is a common biomedical procedure, its pr

150 Irreversible electroporation is a novel, nonthermal ablation modality

151 Electroporation is a widely used technique to permeabili

152 Continuous cell electroporation is an appealing non-viral approach for g

153 Electroporation is an electro-physical, non-viral approa

154 Electroporation is commonly used to deliver molecules su

155 The potential application of CSMEN in electroporation is confirmed by analyzing crystallograph

156 In this way, cell size specific electroporation is conveniently carried out, contributin

157 tical step for initiating vesicle opening by electroporation is diffusion of membrane proteins away f

158 These data support the hypothesis that electroporation is the primary force for pore opening in

159 Electroporation is used to create pores within the membr

160 Our data show that electroporation is well-suited to deliver synthetic and

161 adult rat cerebral cortex following in utero electroporation (IUEP) at embryonic stage E14.

162 enpj during cortical development by in utero electroporation knockdown and found that silencing Cenpj

163 n of Fat1 in cortical precursors by in utero electroporation leads to overproliferation of radial gli

164 ells, delivery of the BH4-Bcl-XL peptide via electroporation limits agonist-induced mitochondrial Ca(

165 Many techniques such as electroporation, lipofection or microinjection have been

166 challenge remaining is that most commercial electroporation machines are built for research and proc

167 Here we developed a new micropillar array electroporation (MAE) platform to advance the electropor

168 Calcium electroporation may offer a simple general tool for anti

169 Electroporation-mediated chemotherapy, known as electroc

170 body-based prophylaxis/therapy entailing the electroporation-mediated delivery of synthetic DNA plasm

171 We have previously reported that electroporation-mediated transuterine gene transfer of C

172 lectroporation phenomenon, or magneto-elasto-electroporation (MEEP), is discovered while studying the

173 The transfection using an electroporation method and genetic manipulation of B. mi

174 describe development of a robust and simple electroporation method in S. oneidensis that allows an e

175 within the intact mouse oviduct by a simple electroporation method, and result in the desired geneti

176 ducible factor-1 alpha (HIF-1alpha) by using electroporation method, specifically optimized for CSCs

177 f cytoreagents.Current widely used viral and electroporation methods for creating therapeutic cell-ba

178 However, the viral and electroporation methods used to create cytoreagents are

179 Compared to lipofection and electroporation methods, plasma transfection showed a be

180 uring scaling down the size of electrodes in electroporation microchips.

181 put, or generate fluorescent vesicles (e.g., electroporation, microinjection, or membrane transductio

182 Moreover, using an in utero electroporation model, we observed that neurons with blo

183 cryoablation, hyperthermia, or irreversible electroporation) (n = 13).

184 We present a simple nanopore-electroporation (NanoEP) platform for delivery of nuclei

185 encoding green fluorescent protein following electroporation of 3T3 fibroblasts.

186 y defective upon loss of either Lhx2 or Ldb1 Electroporation of a chimeric construct that encodes the

187 e-mediated recombination as well as in utero electroporation of a Cre-expression construct identified

188 lencing in mouse embryonic brain by in utero electroporation of a specific Lmnb1 sh-RNA results in ab

189 manufactured ex vivo by cotransfection using electroporation of Cas9 and single guide RNA plasmids.

190 Here, using electroporation of Cas9 nuclease/single-guide RNA ribonu

191 We performed electroporation of CD34+ hematopoietic stem and progenit

192 of LRRTM4 in BCs by subretinal injection and electroporation of CRISPR/Cas9, LRRTM4 was abolished or

193 ence-based methods to demonstrate successful electroporation of E. coli.

194 k-in mutagenesis can be further increased by electroporation of embryos derived from Cas9-expressing

195 Finally, targeted electroporation of Fgfr3 siRNA to prechordal mesoderm in

196 Electroporation of Frmpd1 promoter region, -505 to +382

197 For the first 3 d after electroporation of HCV RNA, intracellular virus predomin

198 -BE3 base editor for ribonucleoprotein (RNP) electroporation of human-peripheral-blood-mobilized CD34

199 In utero electroporation of L1-80 into reeler embryos normalised

200 n follow-up of 20 months, focal irreversible electroporation of localized prostate cancer was associa

201 -HSC editing at single-cell resolution using electroporation of modified synthetic gRNAs and Cas9 pro

202 riants was assessed by luciferase assays and electroporation of mouse retinal explants with reporter

203 Conversely, overexpression of miR-29 by electroporation of mouse tibialis anterior muscle decrea

204 Here using in utero electroporation of NDE1 short hairpin RNA (shRNA) in emb

205 Electroporation of Nfia promoted the formation of cells

206 After electroporation of oncogenic plasmids, mice developed a

207 Furthermore, in vivo electroporation of PRCD C2Y mutant in the mouse retina d

208 tured primary naive murine CD8(+) T cells by electroporation of recombinant Cas9/sgRNA ribonucleoprot

209 sferase activity, in vitro farnesylation and electroporation of recombinant Spindly faithfully result

210 This research aims to evaluate whether the electroporation of Rhodotorula glutinis fresh biomass im

211 primary equine fetal liver cultures or after electroporation of selectable replicons.

212 Our detailed protocol for electroporation of shRNAs in H. symbiolongicarpus embryo

213 Here, we used in utero electroporation of shRNAs or LIC functional domains to d

214 AICD delivery or APP knock-down by in utero electroporation of shRNAs with whole-cell electrophysiol

215 nt knockdown of USP14 or UCHL5 expression by electroporation of siRNA reduced the viability of multip

216 indel and knock-in allele can be achieved by electroporation of small single-guide RNAs and ssODN rep

217 strate that simple intramuscular delivery by electroporation of synthetic DNA plasmids engineered to

218 rs to find the optimal set of conditions for electroporation of target species.

219 Here we show that electroporation of transcription activator-like effector

220 the glucocorticoid receptor (GR) gene using electroporation of transcription activator-like effector

221 Here we report that combining electroporation of zinc finger nuclease (ZFN) mRNA with

222 Our methodology, designated as CRISPR RNP Electroporation of Zygotes (CRISPR-EZ), enables highly e

223 eleventh strand of the GFP protein either by electroporation or by cell-penetrating peptide-mediated

224 ed with guide RNA complementary to omega1 by electroporation or by transduction with lentiviral parti

225 Since it does not require electroporation or microinjection, this tool has the pot

226 hed transposon-based protocols which require electroporation or microinjection.

227 , the system enables on-chip optimization of electroporation parameters for cells with varying proper

228 The optimized electroporation parameters reported here provide a usefu

229 For traditional electroporation parameters, larger cells experience a gr

230 The cell electroporation phenomenon and its correlation with the

231 A magnetically controlled elastically driven electroporation phenomenon, or magneto-elasto-electropor

232 ection procedure was developed that involves electroporation, plasmids replication in mammalian cells

233 CAPZB2 expressed by in utero electroporation prevented normal elongation of vestibula

234 In conclusion, DNA vaccination by electroporation primed for TCR clonotypes that were asso

235 is a key parameter for the liposome membrane electroporation process and hence for the release and ox

236 Our electroporation protocol allows for the transfection of

237 Our electroporation protocols result in payload delivery to

238 ectroporation facilitates the development of electroporation protocols that minimize Joule heating an

239 compositions are critical for the design of electroporation protocols to maximize viability and eTE.

240 nd frequency-dependent effects in developing electroporation protocols, and our approach provides, to

241 eadily take up pyruvate, the addition of the electroporation pulse to the D-DNP experiment increases

242 ion threshold than conventional irreversible electroporation pulse trains, at the expense of larger a

243 ates the cell death response to conventional electroporation pulsed-electric fields ( approximately 1

244 gement of patients with atrial fibrillation: electroporation (pulsed-field ablation), expandable latt

245 mains unaffected 2 months after irreversible electroporation, purposely targeting the adventitia.

246 g(2+)-based buffers expanding the reversible electroporation range.

247 plasmid expressing Cav-1 using transthoracic electroporation reduced infiltration of neutrophils and

248 of cell membranes by pulsed electric fields (electroporation) remain obscure despite decades of inves

249 etween cell morphology, pulse frequency, and electroporation response.

250 es in developing mouse brains using in utero electroporation resulted in abnormal pyramidal neuron mo

251 ates blind (non-visually guided) single-cell electroporation (SCE) and extracellular electrophysiolog

252 Irreversible electroporation seems to be a safe modality for catheter

253 Electroporation serves as a promising non-viral gene del

254 We therefore leveraged the in vivo electroporation strategy used in utero in rodents and in

255 taneously using a CRISPR/Cas9 in vivo retina electroporation strategy.

256 ed triboelectric nanogenerator (TENG)-driven electroporation system is developed for intracellular dr

257 eveloped a versatile on-chip vortex-assisted electroporation system, engineered to conduct sequential

258 ation in mouse corticogenesis using in utero electroporation targeted to projection neurons.

259 design of CRISPR donor with the new oviduct electroporation technique i-GONAD.

260 NA delivery largely outperforms the standard electroporation technique, opening a new avenue for func

261 eported a delayed increase of sensitivity to electroporation (termed "electrosensitization") in mamma

262 erties but to date has not been addressed by electroporation theory or MD simulations.

263 The use of electroporation therefore extends the applicability of D

264 e TMP of tightly packed cells to a simulated electroporation threshold than conventional irreversible

265 Reversible electroporation thresholds were 54% lower than LTs for s

266 eveals a new, to our knowledge, mechanism of electroporation through membranes containing VGICs.

267 ocess in current bulk electroporation (i.e., electroporation to a large population of cells).

268 s 1.5 d post conception, followed by in vivo electroporation to deliver the components into the zygot

269 increase the cell membrane permeability via electroporation to deliver therapeutic molecules into th

270 e data support the feasibility of using mRNA electroporation to engineer HBV TCR-redirected T cells i

271 ction of NOX2 short hairpin RNA (followed by electroporation to facilitate gene delivery) in atria of

272 We combined the imaging window with in utero electroporation to label and track cell division and mov

273 Using in utero electroporation to manipulate the sumoylation state of F

274 Here, we use in utero electroporation to restrict the DISC1 knock-down to pref

275 species (both sexes) by applying sparse cell electroporation to trace single olfactory receptor neuro

276 parameters inspired by clinical irreversible electroporation treatments.

277 We found that partial irreversible electroporation using 200 pulses of 250 V and 70 mus dur

278 ease of intact yeast proteins for LESA-MS by electroporation using a home-built high-voltage device d

279 tes promise for individual cell trapping and electroporation using low voltage AC fields.

280 y electrode minimizes cellular damage during electroporation via enhancing the localized electrical f

281 fine balance between high flow rate and low electroporation voltage were steered clear.

282 Electroporation was achieved by bursts of 300-ns, 9 kV/c

283 administered intramuscularly and followed by electroporation was assessed in mice.

284 val with CT-guided percutaneous irreversible electroporation was exceeded in participants with locall

285 date labeling of granule neurons via in vivo electroporation, we find that early- and late-born granu

286 Using in utero electroporation, we here report that MBG3 mouse models c

287 Using in utero electroporation, we show here that the IGF-1R is essenti

288 Furthermore, through in utero electroporation, we show that downregulation of TBC1D23

289 by intramuscular administration followed by electroporation, were 100% protective against lethal EBO

290 bution and the toxicity of pH changes during electroporation, which significantly decreases cell viab

291 Co-electroporation with a constitutively active form of PI3

292 rate that conditioning of mammalian cells by electroporation with nanosecond pulsed electric field (n

293 enetic probes into cells, while irreversible electroporation with nanosecond pulses is explored to al

294 Standard electroporation with pulses in milliseconds has been use

295 e cortical upper layers was also affected by electroporation with shRNA targeting IGF-1 receptor.

296 he first on-CMOS demonstration of controlled electroporation with the goal of transfection using huma

297 gold-microtube membranes that can accomplish electroporation with voltage pulses that are orders of m

298 and HPV-18 E6 and E7 proteins, delivered by electroporation, would cause histopathological regressio

299 efficacy was optimized by i.m. delivery via electroporation, yet it remained modest compared with Ad

300 cnidarian Hydractinia symbiolongicarpus via electroporation, yielding effective gene-targeted knockd