ライフサイエンスコーパス: endogenous protein

1 cells and recapitulates the behavior of the endogenous protein.

2 ype protein and are able to compete with the endogenous protein.

3 scribe new PMEL17 antibodies that detect the endogenous protein.

4 al autoimmune encephalomyelitis (EAE) to the endogenous protein.

5 recover through open states from block by an endogenous protein.

6 e biochemical tools capable of targeting the endogenous protein.

7 t to an open-channel block and unblock by an endogenous protein.

8 using newly developed antibodies against the endogenous protein.

9 overexpressed by approximately 14-fold over endogenous protein.

10 completely dependent upon knocking down the endogenous protein.

11 nous Fis1 was expressed in yeast lacking the endogenous protein.

12 little is known about the properties of the endogenous proteins.

13 precipitation assays with both exogenous and endogenous proteins.

14 new perspectives for in-cell EPR studies of endogenous proteins.

15 on and the nonspecific interactions with the endogenous proteins.

16 e are no systematic studies of ileal digesta endogenous proteins.

17 s from a variety of cellular contexts and to endogenous proteins.

18 R), for sensitive and multiplexed imaging of endogenous proteins.

19 ntracellularly and bacterially expressed and endogenous proteins.

20 expressed at levels several fold that of the endogenous proteins.

21 precipitation assays with both exogenous and endogenous proteins.

22 ronal and non-neuronal cells expressing only endogenous proteins.

23 sitivity to drugs but also interactions with endogenous proteins.

24 affect the interactions of microtubules with endogenous proteins.

25 cross talk, autofluorescence, and unlabeled, endogenous proteins.

26 ges in the dynamic nanoscale organization of endogenous proteins.

27 sed that methamphetamine covalently glycates endogenous proteins.

28 ells were consistent with the interaction of endogenous proteins.

29 le new tool for the conditional labelling of endogenous proteins.

30 ally and efficiently label multiple distinct endogenous proteins.

31 ting with and activating as-yet-unidentified endogenous proteins.

32 e fused activation or repression domain with endogenous proteins.

33 irmed by coimmunoprecipitation of tagged and endogenous proteins.

34 and specific degradation of a wide range of endogenous proteins.

35 ibe a versatile toolset for rapid tagging of endogenous proteins.

36 100s to 1000s of simultaneous separations of endogenous proteins.

37 enoxyl radicals that covalently tag proximal endogenous proteins.

38 ectable from the frequencies associated with endogenous proteins.

39 od to measure biomarkers, protein drugs, and endogenous proteins.

40 ele encoding an epitope-tagged version of an endogenous protein, (3) alleles expressing reporter prot

41 al and enzymatic functions of two non-tagged endogenous proteins, actin and RAS GTPase, involved in c

42 1alpha reporter constructs as surrogates for endogenous protein activity and target expression, respe

43 Biosensors that report endogenous protein activity in vivo can be based on envi

44 lows direct, fast, and reversible control of endogenous protein activity, with spatiotemporal resolut

45 we present Trim-Away, a technique to degrade endogenous proteins acutely in mammalian cells without p

46 nce (RNAi) resulted in complete knockdown of endogenous protein after 48 h, followed by cell death af

47 ished that protein aggresome-a collection of endogenous protein aggregates-is an important indicator

48 gregation in budding yeast, identifying >170 endogenous proteins aggregating within minutes of heat s

49 Transfer among labeled subunits revealed the endogenous protein alignment.

50 me-resolved quantitative confocal imaging of endogenous proteins along with a fluid-phase marker to a

51 vector may be used to fluorescently label an endogenous protein (alpha-tubulin).

52 haromyces cerevisiae, a eukaryote that lacks endogenous protein AMPylation.

53 N endogenous loss values could underestimate endogenous protein and AA losses by ~6% (the proportion

54 s verified by confocal immunofluorescence of endogenous protein and confirmed by AROS depletion using

55 Co-immunoprecipitation of synthetic and endogenous proteins and co-elution with gel filtration s

56 h generates full-length Citrine fusions with endogenous proteins and conditional mutants from a singl

57 s unclear precisely how macromolecules (e.g. endogenous proteins and exogenous immunotherapeutics) ac

58 f protein methylation from SDS-PAGE isolated endogenous proteins and identified complications for ass

59 Reciprocal immunoprecipitation (IP) of endogenous proteins and microscopically apparent overlap

60 e respiratory activity prevents digestion of endogenous proteins and RNA, which yields bacteria that

61 Here, we reveal that misfolded endogenous proteins and the human amyloid beta peptide t

62 However, current methods do not utilize endogenous proteins and, therefore, do not quantify the

63 l-length MbSrc4 displays low activity toward endogenous proteins, and it cannot functionally substitu

64 IIC, isoform-specific immunostaining of the endogenous proteins, and two-color total internal reflec

65 sis of the rates at which newly synthesized, endogenous proteins appear within mammalian nucleoli.

66 beling reveals that even severely aggregated endogenous proteins are disaggregated without degradatio

67 The endogenous proteins are expressed in separate chromaffin

68 of the Ov-SPI proteins demonstrates that the endogenous proteins are localized to the basal layer of

69 cherichia coli biotin ligase (BirA), whereas endogenous proteins are not modified.

70 ble in 500 mM NaCl, whereas rods formed from endogenous proteins are significantly less stable in hig

71 tracted neuron recruitment has promoted this endogenous protein as a popular indirect tool to monitor

72 reveal a unique molecular code that defines endogenous proteins as inflammatory stimuli by marking t

73 neuploid strains are prone to aggregation of endogenous proteins as well as of ectopically expressed

74 orozoite arising from epitope tagging of the endogenous protein) as a key regulator for adhesion duri

75 interest to apply native MS to the study of endogenous protein assemblies-i.e., those wherein the co

76 oichiometry and intersubunit connectivity of endogenous protein assemblies-information that is key to

77 nd tissue provides precise quantification of endogenous proteins at the approximately 10 pg/mL level

78 rged residue is a SecYEG determinant for the endogenous proteins ATP synthase subunits a and b and th

79 nformational changes or other alterations in endogenous protein autoantigens are required for recogni

80 atinib-directed NEDDylation occurs for known endogenous protein binders in complex cell lysates.

81 acids are disguised from recognition by the endogenous protein biosynthetic machinery, effectively p

82 e colors for imaging interaction networks of endogenous proteins, but also hold the potential to prov

83 nt of the fluorescent protein is fused to an endogenous protein by in-frame exon traps in the enhance

84 protein is predominately cytoplasmic, as is endogenous protein by subcellular fractionation.

85 The technique, single-cell labeling of endogenous proteins by clustered regularly interspaced s

86 We also observed phosphorylation of endogenous proteins by endogenous IP7 in yeast.

87 mice and factor V Leiden mice), in which the endogenous protein C anticoagulant pathway is disrupted

88 scular blood coagulation (thrombosis) by the endogenous protein C system.

89 In addition, the endogenous proteins can be isolated as a complex by immu

90 ugh autoantibodies are generated against the endogenous protein, CD4 cells are activated through a ne

91 ce nanopores of a size comparable to that of endogenous protein channels, but replicating their affin

92 Endogenous protein citrullination was abundant in P ging

93 or example, acetylation and methylation) and endogenous protein cleavages, especially when combinatio

94 ubunit of the mitochondrial ribosome and the endogenous protein co-fractionated with the 28S subunit

95 Only minor amounts of endogenous proteins coaggregated with IMiQ deposits and

96 Endogenous protein-coding gene and transposon transcript

97 mental approach, we identified and validated endogenous protein-coding transcripts that regulate PTEN

98 Quantitative analysis of endogenous protein colocalization in growth cones after

99 ol, membrane and cellular organelles, and to endogenous protein complexes from animal tissue extracts

100 plication of native MS to the elucidation of endogenous protein complexes has been limited by the dif

101 es and mass spectrometry, allow the study of endogenous protein complexes in Drosophila.

102 Targeting endogenous protein complexes of higher eukaryotes, parti

103 hod allowing simultaneous global analysis of endogenous protein complexes that begins with intact lea

104 es rapid and efficient affinity isolation of endogenous protein complexes with a sensitive native MS

105 of either tagged fusion protein complexes or endogenous protein complexes, illustrated here using the

106 purification and identification of extended endogenous protein complexes.

107 the host protein in the context of different endogenous protein complexes.

108 pportunities for the isolation of sensitive, endogenous protein complexes.

109 Endogenous protein concentrations were determined by com

110 , these workflows allow the determination of endogenous protein concentrations.

111 Here, we repurpose an endogenous protein degradation system to rapidly remove

112 D-tagged protein can functionally replace an endogenous protein depleted by RNAi, leading to an induc

113 e we show, by isoform-specific knockdown and endogenous protein detection, along with transgene expre

114 hat cell type-specific nanoscale analysis of endogenous protein distribution is possible in brain cir

115 these ORF proteins were used to localize the endogenous proteins during chlamydial infection.

116 t preventing crosslinking within and between endogenous proteins during hydrogel-tissue hybridization

117 em was used to ablate expression of specific endogenous proteins (e.g., TRAF6; Rb), and was shown to

118 However, only a handful of endogenous proteins encoded by putative lncRNAs have bee

119 -expression leads to increased levels of the endogenous proteins encoded by this co-bound subset of m

120 odent malaria parasite, P. berghei, in which endogenous proteins engineered to contain the auxin-indu

121 The purpose of this study was to identify endogenous protein enhancers using yeast two-hybrid, co-

122 mph DAFPs as "bait" in an effort to identify endogenous protein enhancers.

123 e also introduce a new method for decreasing endogenous protein expression in neurons.

124 we identified multiple miRNAs that repressed endogenous protein expression of both genes, seed-based

125 t these same sites are phosphorylated in the endogenous protein extracted from Limulus lateral eye, a

126 ntial sources, as follows: newly synthesized endogenous proteins for direct presentation on the surfa

127 hnique, we are able to examine low levels of endogenous protein from cell extracts without the need f

128 ulation is accompanied by a depletion of the endogenous protein from its normal localization.

129 lowed by MS for their ability to capture the endogenous protein from mammalian cell lysates.

130 was demonstrated by coimmunoprecipitation of endogenous proteins from brain tissue and shown to be di

131 jA was confirmed by coimmunoprecipitation of endogenous proteins from flies, and the endogenous prote

132 Using specific antibodies to detect endogenous proteins from human and mouse cells, we found

133 as confirmed by coimmunoprecipitation of the endogenous proteins from human HepG2 or CaSki cells, alt

134 Interaction proteomics analysis of endogenous proteins from the cellular model, mainly in h

135 inducible, and allele-targeted inhibition of endogenous protein function, and the ability to titrate

136 n or transiently (within 1 h) by tagging the endogenous protein genomically with the auxin degron.

137 Drug covalent binding to endogenous proteins (haptenation) is thought to be requi

138 The endogenous protein has 88.0% sequence homology with the

139 thods faithfully report on the properties of endogenous proteins has not been studied comparatively.

140 owever, extracellular morphogen gradients of endogenous proteins have not been directly visualized in

141 Proteomic analysis of rods formed from endogenous proteins identified other potential component

142 otting, corresponding to the localization of endogenous protein in cytoplasmic vesicular structures b

143 The presence of endogenous protein in dinosaur bone may validate hypothe

144 correlates with retarded degradation of the endogenous protein in wild-type plants and prolonged epi

145 or analysis and the ability to work with the endogenous proteins in a given sample (e.g., stabile iso

146 s show relatively little cross-reaction with endogenous proteins in a variety of monocotyledonous and

147 In this study, by fluorescently labeling endogenous proteins in Caenorhabditis elegans embryos, w

148 scribe a method for absolute quantitation of endogenous proteins in cell lysates using an automated c

149 tags could allow superresolution analysis of endogenous proteins in discrete conformational states.

150 ins the measured robustness of synthesis for endogenous proteins in E. coli.

151 confirmed the interaction between these two endogenous proteins in endothelial cells.

152 The interactions with endogenous proteins in human cell extracts were confirme

153 dy the physical and biological properties of endogenous proteins in human cells without the need for

154 o-immunoprecipitation in Cos7 cells and with endogenous proteins in human neuroblastoma cells.

155 an embryonic kidney (HEK) 293 cells and with endogenous proteins in human neuroblastoma cells.

156 unoprecipitation in mammalian cells and with endogenous proteins in JEG3 placental cells, neuroblasto

157 ormat, small-molecule target engagement with endogenous proteins in live cells can be quantified.

158 The ability to visualize endogenous proteins in living neurons provides a powerfu

159 munoprecipitation/Western blot analysis with endogenous proteins in MEL cells and heterologous protei

160 s strategy, we achieved efficient tagging of endogenous proteins in primary and organotypic cultures

161 quilin 1 interaction can be detected between endogenous proteins in primary neurons in vitro as well

162 periments demonstrate an interaction between endogenous proteins in rat brain cytosol.

163 e absolute quantification of therapeutic and endogenous proteins in the biopharmaceutical industry ov

164 with the finding that association of the two endogenous proteins in the cell extract chromatin fracti

165 We found that interaction of the two endogenous proteins in the differentiated muscle fibers

166 ion of the localization and dynamics of many endogenous proteins in various cell types, regions, and

167 Wnt activity is antagonized by endogenous proteins including dickkopf-1 (DKK-1).

168 opposite chirality and thus protection from endogenous proteins including proteases.

169 egral sarcolemmal phosphoprotein, is a novel endogenous protein inhibitor of cardiac NCX1.

170 TopAI is the first endogenous protein inhibitor specific for topoisomerase

171 tivity is posttranscriptionally regulated by endogenous protein inhibitors (PMEIs).

172 e association of PP1 catalytic subunits with endogenous protein inhibitors and regulatory subunits.

173 A family of endogenous protein inhibitors of calcineurin was recentl

174 d reporter polypeptides as well as misfolded endogenous proteins inside mitochondria takes place via

175 ting enzyme UBE2D3 with substrate PCNA), and endogenous proteins interacting with thioredoxin in E. c

176 eins of interest, enabling the monitoring of endogenous protein interactions or detection of a single

177 complementation to be expanded to monitoring endogenous protein interactions.

178 enerative disease caused by misfolding of an endogenous protein into an alternative pathogenic confor

179 aggregation-prone proteins can convert other endogenous proteins into drivers of aggregation and toxi

180 The conversion of exogenous and endogenous proteins into immunogenic peptides recognized

181 ers characterized by the aberrant folding of endogenous proteins into self-propagating pathogenic con

182 novel mouse strain, RanBP9-TT, in which the endogenous protein is fused with a double (V5-HA) epitop

183 express GFP in the normal tissues where the endogenous protein is present.

184 identify precise subcellular localization of endogenous proteins is essential for integrative underst

185 d peptides with phosphopeptides derived from endogenous proteins isolated from cells in which the kin

186 induce the rapid degradation of the specific endogenous protein itself.

187 The data show further that endogenous protein kinase A is a primary catalyst of cel

188 ase C competitively with diacylglycerol, the endogenous protein kinase C regulator, and plant-derived

189 ice, including: differences in regulation by endogenous protein kinase C, differential sensitivity to

190 Endogenous protein kinase CK2 purifies with Bdf1 and pho

191 regulation of PKA signaling, members of the endogenous protein kinase inhibitor (PKI) family may be

192 eals that the proteasome can be activated by endogenous protein kinases, making it possible to pharma

193 nts from the nanoparticle surface results in endogenous protein knockdown simultaneously with exogeno

194 Endogenous protein levels for these Wnt antagonists are

195 nteractions are of functional consequence at endogenous protein levels has remained unclear so far.

196 A method that directly alters endogenous protein levels is currently not available.

197 ow that Pyk2 increases promoter activity and endogenous protein levels of involucrin, a marker of ker

198 or NHERF2 caused a significant reduction in endogenous protein levels of SR-BI.

199 In muscles from Cmpt mice, endogenous protein levels of STIM1 and Orai1 were reduce

200 ciate with Aph-1 at overexpressed as well as endogenous protein levels.

201 Drosophila Toll receptor is activated by the endogenous protein ligand Spatzle in response to microbi

202 es at the proximal heme face in place of the endogenous protein ligand.

203 nstrated as a promising tool for identifying endogenous protein-lipid interactions.

204 , using quadruple immunolocalization against endogenous proteins, live imaging of dendritic endosomes

205 Analysis of endogenous protein localization, function, and dynamics

206 versal genome engineering (HiUGE) method for endogenous protein manipulation that is straightforward,

207 co-immunoprecipitation of overexpressed and endogenous proteins, maps to the amino-terminal 70 resid

208 convenient probe to study the activities of endogenous protein methyltransferases.

209 Our findings raise the possibility that endogenous protein modifications that disrupt the M80-Fe

210 ate the mechanisms and the kinetics of these endogenous protein modifications, we used a dynamic arti

211 While many of the endogenous proteins modified by O-Fuc are important for

212 relevant to unravel mechanisms making other endogenous proteins more vulnerable to undergo conformat

213 techniques can reliably image the changes in endogenous protein nanoclustering dynamics associated wi

214 he molecular mechanism of TLR9 activation by endogenous protein-nucleic acid complexes, which are ass

215 st, lysosomal trafficking of endocytosed and endogenous proteins occur via different routes and/or ar

216 ty, we studied the effects of overexpressing endogenous proteins on disulphide bond formation in the

217 med cells must present peptides derived from endogenous proteins on MHC class I molecules to be recog

218 ty biotinylation, we captured and identified endogenous proteins on the outer mitochondrial membrane

219 yBL is extremely sequence-specific, as endogenous proteins on the surface of yeast and HeLa cel

220 gs without inhibiting the Ag presentation of endogenous protein or peptides.

221 lication to the absolute quantitation of any endogenous protein or protein-based drug in tissue.

222 Here we introduce new approaches to quantify endogenous protein organization at single-molecule resol

223 Cytosolic peptidases degrade endogenous proteins originating from self or pathogens,

224 BSco) has a histidine (H135) and an unknown endogenous protein oxygen ligand in a distorted tetragon

225 Unexpectedly, delivery of endogenous proteins, p67 and TbCatL, were unaffected ind

226 eriments were used to confirm several of the endogenous protein partners of BRD7 and BRD9, which form

227 uggest that, despite the lack of full-length endogenous protein, patients with PTC-inducing non-sense

228 (flg22), elongation factor-Tu (elf18), or an endogenous protein (pep1).

229 Understanding why the endogenous protein PF4 becomes immunogenic when complexi

230 pha-4 coprecipitated hemagglutinin-MEK3 plus endogenous protein phosphatase 2A (PP2A) and selectively

231 ls to the extracellular matrix, and we found endogenous protein phosphatase-1alpha isoform (PP1alpha)

232 in a hippocampal cell line, with >60% of all endogenous protein phosphorylated at this residue.

233 synthesis and receptor components with yeast endogenous protein phosphorylation elements and new resp

234 The snake venom protein rhodocytin and the endogenous protein podoplanin have been identified as li

235 uorescent dye, to label and accurately count endogenous proteins present in very low numbers (<7) in

236 -[(32)P]GMP detected an approximately 47-kDa endogenous protein, presumably c-di-GMP downstream recep

237 hroughput quantitative mass spectrometry and endogenous protein-protein interaction revealed an NLRX1

238 of selectively altering the distribution of endogenous proteins, providing a link between intronic S

239 Endogenous protein quality control machinery has long be

240 by biotin ligase added to the medium, while endogenous proteins remain unmodified.

241 e Erv1 into erv1-ts mitochondria lacking the endogenous protein restores import and assembly of the s

242 orbital-lowering intermediate similar to the endogenous protein rhodopsin effectively catalyzes isome

243 pid immunoprecipitation mass spectrometry of endogenous protein (RIME) assay, we identified RuvB-like

244 pid immunoprecipitation mass spectrometry of endogenous protein (RIME) is a method that allows the st

245 for a sufficient time to reduce the level of endogenous protein, should be generally useful for targe

246 cently tagged proteins and immunostaining of endogenous proteins showed that NM2 and M18A are present

247 rinary elimination, based on manipulating an endogenous protein:siderophore:iron clearance pathway.

248 , whereas the latter can detect thousands of endogenous proteins simultaneously, but only in lysed sa

249 metics, because they mimic the action of the endogenous protein Smac/Diablo that antagonizes cIAP fun

250 cross-reactions between externally added and endogenous protein species.

251 e-preserving ExCel, which enables imaging of endogenous proteins stained by antibodies, and iterative

252 e predicted factor as a new 1,277-amino-acid endogenous protein (STAMP).

253 were found to prevent BoNT/A cleavage of the endogenous protein substrate, SNAP-25, even at low muM c

254 e site, thus impeding its proteolysis of the endogenous protein substrate, synaptosomal-associated pr

255 Diseases associated with the misfolding of endogenous proteins, such as Alzheimer's disease and typ

256 r medically relevant imaging because certain endogenous proteins, such as muscle myosin lattices with

257 Gel filtration of the endogenous protein suggested that its predominant struct

258 man cryosectioned podocytes may reflect both endogenous protein synthesis and APOL1 uptake from the c

259 We visualized endogenous protein synthesis and demonstrated that seizu

260 This method allows for robust analysis of endogenous protein synthesis in a cell-type-specific man

261 imaging also revealed specific inhibition of endogenous protein synthesis in the SAT1 overexpressing

262 nthesis, it has not been possible to measure endogenous protein synthesis levels in vivo in an entire

263 ter, as well as a small-molecule reporter of endogenous protein synthesis, we show that dopamine D1/D

264 are not substrates for SAT1 restored GFP and endogenous protein synthesis.

265 These peptides can be generated from either endogenous proteins synthesized by the biosynthetic mach

266 In an endogenous protein tagging assay, lon mutants accumulate

267 We have demonstrated dual-color endogenous protein tagging with sfCherry211 and GFP11, r

268 RNA tag and extensive ladder-like pattern of endogenous protein-tagging events in F. tularensis that

269 We use this to determine the effects of endogenous protein tags or drugs on the shape dynamics o

270 High molecular weight kininogen (HK) is an endogenous protein that is proteolytically cleaved in pl

271 The existence of endogenous proteins that act as "anti-chaperones" uncove

272 of mitochondria, with cell death executed by endogenous proteins that act to increase the permeabilit

273 itylation of AgDD itself, along with that of endogenous proteins that are components of the ribosome

274 e catalyzes the biotinylation of neighboring endogenous proteins that can then be isolated and identi

275 nase-2 (TIMP-2) belongs to a small family of endogenous proteins that inhibits a group of enzymes, th

276 embly of a synthetic peptide for trafficking endogenous proteins, this work provides insights for und

277 Interactions are confirmed for chimeric and endogenous proteins through quantitated in vitro and cel

278 ntercellular movement of GFP and a subset of endogenous proteins through RCs during spermatogenesis,

279 reporter constructs and Western blotting of endogenous protein to verify that miR-125b targets Suv39

280 HPV type 16 (HPV16) E6 interacts with endogenous proteins to activate hTERT, the catalytic sub

281 ave quantified the responses of thousands of endogenous proteins to clinical PARP inhibitors Olaparib

282 A-I in humans) present peptides derived from endogenous proteins to CTLs.

283 ncentration is a function of dietary intake, endogenous protein turnover and catabolism of the three

284 further supported by co-localization of the endogenous proteins using confocal microscopy.

285 ecipitation and proximity ligation assays on endogenous proteins, we demonstrate that Parkin interact

286 In this study using purified endogenous proteins, we examined the interaction between

287 s from WT and telokin KO mice as a source of endogenous proteins, we found that only in the presence

288 In previous work with endogenous proteins, we identified that Sac3, a phosphat

289 n of endogenous proteins from flies, and the endogenous proteins were found to colocalize at the trac

290 alance between immunological clearance of an endogenous protein with acquired toxic properties and th

291 These two models were tested by replacing endogenous protein with ZP2 that cannot be cleaved (Zp2(

292 This study explores whether tagging endogenous proteins with a reporter is a scalable strate

293 enome-editing strategy to systematically tag endogenous proteins with fluorescent tags in human induc

294 simple and generalizable technique to image endogenous proteins with high specificity, resolution, a

295 on of protein signaling, as they can replace endogenous proteins with minimal perturbation of native

296 D), which can be used to fluorescently label endogenous proteins with near ideal properties in all ne

297 receptors and protease-activated receptors, endogenous proteins with protective effects such as the

298 It combines RNAi-mediated silencing of two endogenous proteins with the expression of two exogenous

299 tform to map the subcellular localization of endogenous proteins with the resolution of micro- to nan

300 ly and temporally resolved proteomic maps of endogenous proteins within living cells.