ライフサイエンスコーパス: endoglycosidase

1 Heparanase is a heparan sulfate degrading endoglycosidase.

2 ns are first deglycosylated with a wild-type endoglycosidase.

3 in SDS-PAGE before and after treatment with endoglycosidase.

4 e supernatant, indicating the presence of an endoglycosidase activity.

5 lglucosamine (GlcNAc) of IgG, using a mutant endoglycosidase (also called endoglycosynthase) that lac

6 Finally, N-glycan profiling and endoglycosidase analyses showed that the vast majority o

7 ompounds were identified by a combination of endoglycosidase and exoglycosidase digestions, anion-exc

8 ng advantage of the specificity of different endoglycosidases and isotope dimethyl labeling, six N-gl

9 ere determined by treating each protein with endoglycosidases and then subjecting it to sodium dodecy

10 Protein endoglycosidases are useful for biocatalytic alteration

11 The Endo-F3 mutants represent the first endoglycosidase-based glycosynthases capable of transfer

12 o mutants described here represent the first endoglycosidase-based glycosynthases enabling a highly e

13 In this article, we report studies of two endoglycosidase-based glycosynthases, EndoM-N175A and En

14 from Streptococcus pneumoniae (Endo-D) is an endoglycosidase capable of hydrolyzing the Fc N-glycan o

15 fringens ATCC10543 were contaminated with an endoglycosidase capable of releasing the disaccharide Gl

16 Glycosylation remodeling using the endoglycosidase-catalyzed deglycosylation and transglyco

17 We describe herein the optimization of an endoglycosidase-catalyzed glycosylation of the best-sell

18 ha-mannosidase inhibitor kifunensine, and an endoglycosidase-catalyzed glycosylation of the deglycosy

19 Endoglycosidase-catalyzed in vitro glycoengineering tech

20 in vitro enzymatic glycosylation, using the endoglycosidase-catalyzed transglycosylation as the key

21 e containing two GlcNAc residues and a novel endoglycosidase-catalyzed transglycosylation that simult

22 the GlcNAc-primer in the IgG1-Fc through an endoglycosidase-catalyzed transglycosylation, using suga

23 is of novel N-glycan clusters using a tandem endoglycosidase-catalyzed transglycosylation.

24 Heparanase, an endoglycosidase, colocalized with perlecan in the baseme

25 eral hours to overnight) and relatively high endoglycosidase concentration (from 1:250 to 1:500 enzym

26 d here provides a framework from which novel endoglycosidases could be engineered for additional clin

27 utant TREM2 becomes sensitive to cleavage by endoglycosidase D under conditions that inhibit recyclin

28 Hyaluronidase (HAase), an endoglycosidase, degrades HA into small angiogenic fragm

29 Heparanase is an endoglycosidase degrading heparan sulfate, of the baseme

30 sylation, in contrast to previously reported endoglycosidases derived from Endo-S, Endo-M, Endo-D, an

31 Surface biotinylation and endoglycosidase digestion experiments showed that TcGP63

32 Endoglycosidase digestion followed by SDS/PAGE, lectin a

33 ither tunicamycin treatment of the cells nor endoglycosidase digestion of calreticulin resulted in an

34 lation process inhibitor kifunensine and the endoglycosidase Endo H, and intramuscularly immunized mi

35 We identified an 88-kDa secreted protein, endoglycosidase (Endo) E, which is most likely responsib

36 nes , EndoS, is complementary to other known endoglycosidases (EndoA, EndoH) used for current protein

37 ities of the bacterial protease IdeS and the endoglycosidase EndoS, upregulated during infection.

38 The IgG-specific endoglycosidases EndoS and EndoS2 from Streptococcus pyo

39 analysis using a combination of IdeS and the endoglycosidases EndoS and EndoS2 to comprehensively map

40 ost common human pathogens, secretes a large endoglycosidase, EndoS, which removes carbohydrates in a

41 he immune system, a process catalyzed by the endoglycosidase EndoS2.

42 icken erythroid AE1 anion exchangers receive endoglycosidase F (endo F)-sensitive sugar modifications

43 CD38 HL-60 ADPR-cyclase to inactivation by N-endoglycosidase F and to thermal inactivation at 45 degr

44 ectants after removal of sugar residues with endoglycosidase F or following tunicamycin treatment to

45 und to be resistant to digestion with either endoglycosidase F or H but sensitive to peptide N-glycos

46 with high concentrations of N-glycosidase F, endoglycosidase F, endoglycosidase H, and endo-beta-gala

47 retic mobility was altered by treatment with endoglycosidase F.

48 Da which are converted to 155 and 115 kDa by endoglycosidase F.

49 We modified the N-glycan of SZ21 by endoglycosidase F.

50 Deglycosylation of PHF tangles by endoglycosidase F/N-glycosidase F converts them into bun

51 protein) which was sensitive to tunicamycin, endoglycosidase F/N-glycosidase, and endoglycosidase H b

52 cted by treatment of the cells with trypsin, endoglycosidase F/peptide N-glycosidase F, Vibrio choler

53 o CNS-type NMDAR1 after deglycosylation with endoglycosidase F/peptide-N-glycosidase F.

54 Treatment of platelet membranes with endoglycosidase-F causes the P2X1 receptor band to migra

55 ceptor (obtained by enzymatic treatment with Endoglycosidase-F/N-glycosidase-F).

56 NPR-ECD deglycosylated with endoglycosidase F2 and endoglycosidase H retained ANP-bi

57 sidase assisted analysis using sialidase and endoglycosidase F2/F3, respectively, to improve resoluti

58 labeled with 2-aminobenzamide, digested with Endoglycosidases F2 and H followed by normal phase HPLC

59 Digestion of monoclonal antibodies with Endoglycosidases F2 and H, which cleave the glycosidic b

60 derived from Elizabethkingia meningoseptica endoglycosidase F3 (Endo-F3) of the GH18 family, which a

61 n was determined for core fucosylation using endoglycosidase F3, and differentiation of sialic acid l

62 ed by site-directed mutagenesis of EndoS (an endoglycosidase from Streptococcus pyogenes ) and were f

63 Here we reveal that a bacterial endoglycosidase from Streptococcus pyogenes , EndoS, is

64 a systematic site-directed mutagenesis of an endoglycosidase from Streptococcus pyogenes of serotype

65 We also find that two HM-processing endoglycosidases from the human gut-resident Alistipes f

66 poptotic factors (i.e. Smac/DIABLO, AIF, and endoglycosidase G).

67 Treatment with PNGase F, but not Endoglycosidase H (Endo H) (specific for high-mannose N-

68 was determined by proteinase K digestion and endoglycosidase H (Endo H) cleavage.

69 lex (pgCIII) was sensitive to treatment with endoglycosidase H (endo H), while the mature gCIII compl

70 ted with the presence of a limited amount of endoglycosidase H (Endo H)-resistant HLA-F.

71 pike protein (S) was newly synthesized as an endoglycosidase H (endo H)-sensitive glycoprotein (gp170

72 n-19 is not utilized; Asn-39 is linked to an endoglycosidase H (Endo H)-sensitive oligosaccharide, an

73 alnexin), yet surface TPO molecules remained endoglycosidase H (endo H)-sensitive.

74 s, the N-linked sugars remained sensitive to Endoglycosidase H (Endo H).

75 dases peptide-N-glycosidase F (PNGase F) and endoglycosidase H (Endo H).

76 ficantly reduced in cd81(-/-) B cells and is endoglycosidase H (endo-H) resistant.

77 Endoglycosidase H (endo-H) treatment of particles demons

78 However, endoglycosidase H (endoH) digestion of the viral envelop

79 onnatural sugars at N-glycan core positions, endoglycosidase H (endoH)-digested peptides from a purif

80 nt molecular weights and remained completely endoglycosidase H (endoH)-sensitive, suggesting incomple

81 val, (ii) all detectable processed mMDC15 is endoglycosidase H -resistant, and (iii) a recombinant so

82 Digestion of gp120 with endoglycosidase H abrogates the binding of SP-A, indicat

83 Pulse-chase and endoglycosidase H analysis demonstrate that the iHeps re

84 Cell surface biotinylation and endoglycosidase H analysis revealed a 128-kDa precursor

85 Endoglycosidase H analysis revealed that the modified En

86 cognized by this antiserum were sensitive to endoglycosidase H and associated with calnexin, indicati

87 igomers; however, they remained sensitive to endoglycosidase H and did not undergo endoproteolytic cl

88 were established by pulse-chase analysis and endoglycosidase H and glycopeptidase F digestions.

89 Digestion with N-glycosidase F, endoglycosidase H and lectin blotting did not reveal any

90 Endoglycosidase H and peptide N-glycosidase treatment an

91 nous parasite glycoproteins using sequential endoglycosidase H and peptide:N-glycosidase-F digestions

92 e and mature GC-C glycoforms on the basis of endoglycosidase H and PNGase sensitivities.

93 US7, US9, and US10 remained sensitive to endoglycosidase H and were exclusively or largely presen

94 CPD increases to 180 kDa and is resistant to endoglycosidase H as a result of carbohydrate maturation

95 PP I proenzyme with both N-glycosidase F and endoglycosidase H as well as treatment of the cells with

96 amycin, endoglycosidase F/N-glycosidase, and endoglycosidase H but not to O-glycosidase.

97 , and both mature proteins were resistant to endoglycosidase H but sensitive to glycopeptidase F.

98 Treatment with endoglycosidase H caused an increase in electrophoretic

99 Endoglycosidase H cleaved the 45-kDa band to approximate

100 Effects of tunicamycin and endoglycosidase H confirmed that these novel isoforms re

101 y limited trypsin digestion and treated with endoglycosidase H deglycosidase to reduce heterogeneity

102 ecular mass in SDS-PAGE and was resistant to endoglycosidase H deglycosidase.

103 Peptide N-glycosidase F and endoglycosidase H deglycosylate hENT1 to 37 kDa and hENT

104 but in alpha1(A322D)beta2gamma2S receptors, endoglycosidase H deglycosylated 91 +/- 4% of alpha1(A32

105 In alpha1beta2gamma2S receptors, endoglycosidase H deglycosylated only 26 +/- 5% of alpha

106 However, cell surface biotinylation and endoglycosidase H digestion assays appear to under-repre

107 Endoglycosidase H digestion assays indicated that SALM1D

108 n sensitivity to both mannan competition and endoglycosidase H digestion compared to that of the 124m

109 Pulse-chase analysis and endoglycosidase H digestion of surface-biotinylated MMP-

110 Endoglycosidase H digestion of the mutated TfRs indicate

111 Endoglycosidase H digestion showed that a significant fr

112 on of flow cytometry, patch clamp recording, endoglycosidase H digestion, brefeldin A treatment, and

113 Endoglycosidase H digestion, immunocytochemistry, and su

114 elet Mpl, PV platelet Mpl was susceptible to endoglycosidase H digestion, indicating defective Mpl pr

115 gglutinin at a stage preceding resistance to endoglycosidase H digestion, thus impairing hemagglutini

116 Treatment with endoglycosidase H indicated that Rem-CT does not traffic

117 Experiments employing endoglycosidase H indicated that the early slow processi

118 The acquisition of resistance to endoglycosidase H indicated trafficking of S to the medi

119 Yet, endoglycosidase H insensitivity indicates an aberrant ol

120 By monitoring acquisition of endoglycosidase H resistance after metabolic labeling, w

121 tution mutant S proteins displayed levels of endoglycosidase H resistance and cell surface expression

122 se media reversibly inhibited development of endoglycosidase H resistance and mannose-binding activit

123 ldin A required more than 120 min to acquire endoglycosidase H resistance and maximal binding activit

124 slational processing of these two mutants to endoglycosidase H resistance and very low cell surface e

125 e cell surface have the potential to acquire endoglycosidase H resistance as a consequence of Golgi-b

126 he ER is confirmed by the failure to acquire endoglycosidase H resistance at 37 degrees C and cannot

127 al approach, we found that the onset of VSVG endoglycosidase H resistance in Rab6 depleted cells was

128 d with a 45-min half-time for development of endoglycosidase H resistance.

129 D deglycosylated with endoglycosidase F2 and endoglycosidase H retained ANP-binding activity and show

130 leavage site mutant I was N glycosylated and endoglycosidase H sensitive, indicating that ER transloc

131 cular chaperones BiP and GRP94, and remained endoglycosidase H sensitive, suggesting retention in the

132 emonstrated that the M(r) 85,000 species was endoglycosidase H sensitive, suggesting targeting of the

133 Endoglycosidase H sensitivity assays showed that N-linke

134 munofluorescence microscopy and constitutive endoglycosidase H sensitivity in metabolically labeled c

135 between the two cell lines as determined by endoglycosidase H sensitivity of newly sensitized P-glyc

136 al immature glycosylation, manifest by their endoglycosidase H sensitivity.

137 dopsin as judged by mobility on SDS/PAGE and endoglycosidase H sensitivity.

138 We used the glycosidase endoglycosidase H to determine that this difference in m

139 tor N-butyldeoxynojirimycin and treated with endoglycosidase H to remove all but the terminal N-acety

140 We have used endoglycosidase H to remove the Asn-linked glycans from

141 d material to dense lysosomes was delayed in endoglycosidase H treated cells, but the rate of degrada

142 Pulse-chase experiments in combination with endoglycosidase H treatment determined that the mutant m

143 Endoglycosidase H treatment of microsomes containing nas

144 d be inhibited by d-mannose and abolished by endoglycosidase H treatment of THP, suggesting that the

145 Endoglycosidase H treatment of ts045VSVG-GFP confirmed t

146 Endoglycosidase H treatment resulted in the removal of o

147 In addition to transcription/translation, endoglycosidase H treatment was used to verify glycosyla

148 class I molecules exhibiting sensitivity to endoglycosidase H treatment.

149 ion, we determined that N-glycan removal via endoglycosidase H(f) treatment of TconTS1 led to a decre

150 and only the 93-kDa wt C2 were sensitive to endoglycosidase H, a marker for transport from the endop

151 tions of N-glycosidase F, endoglycosidase F, endoglycosidase H, and endo-beta-galactosidase.

152 ith neuraminidase, resistance to cleavage by endoglycosidase H, and GalNAc-independent labeling with

153 mutant receptor to peptide-N-glycanase F and endoglycosidase H, and insensitivity to sialidase indica

154 138 and Asn-175 were completely sensitive to endoglycosidase H, and they were phosphorylated.

155 porate the J chain and resist degradation by endoglycosidase H, arguing for IgM passage through the G

156 from mPmel17 not only in its sensitivity to endoglycosidase H, but also in the content of core 1 O-g

157 Peptide N-glycosidase F, but not endoglycosidase H, digestion converted all beta1 to an a

158 treatment of target cells with tunicamycin, endoglycosidase H, or protease (pronase).

159 ome processed fertilin alpha is sensitive to endoglycosidase H, suggesting cleavage occurs prior to t

160 lted in some enzyme that lost sensitivity to endoglycosidase H, suggesting that the first disulfide b

161 TR-infected cells, gO remained sensitive to endoglycosidase H, suggesting that the protein was not e

162 toreceptors, P/rds was robustly sensitive to endoglycosidase H, which is consistent with its bypassin

163 In this study, we describe an endoglycosidase H-deglycosylated form of TPP1 containing

164 The structures, determined at 1.85 A for the endoglycosidase H-deglycosylated protease domain produce

165 n the Cbeta domain, was thrombin-cleaved and endoglycosidase H-digested, and the two derivatives were

166 ecules are delayed in their maturation to an endoglycosidase H-resistant form after biosynthetic labe

167 ed ADAM3 exhibited an increased amount of an endoglycosidase H-resistant form that may be related to

168 nverted to the Golgi-processed and therefore endoglycosidase H-resistant form, and the endoglycosidas

169 he Ostalpha subunit to a mature glycosylated endoglycosidase H-resistant form, suggesting that co-exp

170 ed medium, these chains were converted to an endoglycosidase H-resistant form.

171 SP-B and SP-BDeltaC remained in cells in an endoglycosidase H-resistant form.

172 dified by N-linked glycosylation to a mature endoglycosidase H-resistant form.

173 t product being a relatively unstable 24-kDa endoglycosidase H-resistant glycoprotein.

174 pancies of numerous N-glycosylation sites by endoglycosidase H-resistant N-glycans originating from M

175 rporated into POMC predominantly on N-linked endoglycosidase H-resistant oligosaccharides.

176 alpha subunit, but not non-cleaved alpha, is endoglycosidase H-resistant.

177 cosylation sites and unknown numbers of both endoglycosidase H-sensitive and -resistant oligosacchari

178 Endoglycosidase H-sensitive class I molecules were detec

179 l cytoplasmic domain show that they exit the endoglycosidase H-sensitive compartment at a slower rate

180 x glycosylation of wild-type AQP2 but mainly endoglycosidase H-sensitive core glycosylation of AQP2-T

181 re endoglycosidase H-resistant form, and the endoglycosidase H-sensitive ER form has a half-life of 2

182 retained in the endoplasmic reticulum in an endoglycosidase H-sensitive form associated with the mol

183 rbohydrate chains of SCAP were mostly in the endoglycosidase H-sensitive form when cells were grown i

184 s of S and MHVR via coimmunoprecipitation of endoglycosidase H-sensitive forms of the two proteins.

185 within the endoplasmic reticulum as immature endoglycosidase H-sensitive forms.

186 ells, CPD is initially produced as a 170-kDa endoglycosidase H-sensitive glycoprotein.

187 crystals were composed of correctly folded, endoglycosidase H-sensitive IgG.

188 sed surface IgM coupled with accumulation of endoglycosidase H-sensitive IgM intracellularly, resembl

189 NAc2-PP-dolichol transferred by TbSTT3A, and endoglycosidase H-sensitive N-glycans originating from M

190 ocol; describes the presence of full-length, endoglycosidase H-sensitive NSP4 in plasma membrane cave

191 The enzyme contains endoglycosidase H-sensitive oligosaccharides that contai

192 d-type gE were synthesized as N-glycosylated endoglycosidase H-sensitive precursors with approximate

193 This Man(6)GlcNAc(2) is the endoglycosidase H-sensitive product of the Alg3p step.

194 peared mostly as a 70-kDa core-glycosylated, endoglycosidase H-sensitive, immature form bound to the

195 ome cells, it is sensitive to digestion with endoglycosidase H.

196 as assessed by acquisition of resistance to endoglycosidase H.

197 ecular weight form and gained sensitivity to endoglycosidase H.

198 mmature form was sensitive to digestion with endoglycosidase H.

199 se and O-glycanase but not to treatment with endoglycosidase H.

200 s a 45-kDa glycoprotein that is sensitive to endoglycosidase-H and is reduced to 37-kDa after N-glyca

201 Endoglycosidase-H digests and colocalization with endopl

202 ling studies demonstrate that acquisition of endoglycosidase-H resistance of CD1d is observed in the

203 cells, however, Glyco-PDIA1 is released with endoglycosidase-H sensitive glycans, implying that it do

204 and gamma(c) chains associated with p12I are endoglycosidase-H sensitive, suggesting that their inter

205 is expressed on the cell surface as a 48-kDa endoglycosidase-H-resistant glycoprotein.

206 es, but the currently limited selectivity of endoglycosidases has prevented effective manipulation of

207 The endoglycosidase heparanase specifically cleaves the hepa

208 ell-associated molecules leads to sequential endoglycosidase (heparanase) fragmentation of the chains

209 dels to investigate the contributions of the endoglycosidase HYAL1 and the exoglycosidase beta-hexosa

210 Introduction of both the engineered mAb and endoglycosidase in serum leads to a dramatic increase in

211 Inhibition of heparanase (an HS endoglycosidase) in in vitro cultured HFs has been shown

212 ectivity for amylases over related retaining endoglycosidases is validated by structural studies.

213 cellular production of functional vIL-6, but endoglycosidase-mediated removal of N-linked glycans fro

214 eless, neither Endo-A nor the Mucor hiemalis endoglycosidase mutants (EndoM-N175A and EndoM-N175Q) we

215 pact on antibody effector function, with the endoglycosidases of Streptococcus pyogenes deglycosylati

216 to short glycosaminoglycans by the action of endoglycosidases or heparanases.

217 Application of an endoglycosidase, peptide N-glycosidase F (PNGaseF), dire

218 ed peptides, and the glycans released by the endoglycosidase PNGase F.

219 The endoglycosidase promotes infected cell survival and supp

220 rious glycosyltransferases, also includes an endoglycosidase, PslG.

221 Endoglycosidase S (EndoS) is a glycoside-hydrolase secre

222 -chain deglycosylation with bacteria-derived endoglycosidase S (EndoS).

223 Specifically, we show that (i) EndoS (endoglycosidase S) cleaves only complex-type glycans of

224 First, we expressed endoglycosidase S2 in Expi293F GnT1- cells to trim all N

225 st in two distinct glycoforms with different endoglycosidase sensitivities.

226 dentified by molecular weight determination, endoglycosidase sensitivity, and microsequencing analysi

227 Endoglycosidase studies demonstrated that both products

228 The actions and specificities of endoglycosidases such as a peptide-N-glycosidase F (PNGa

229 Heparanase is an endoglycosidase that cleaves heparan sulfate side chains

230 Heparanase (the only known mammalian endoglycosidase that cleaves heparan sulfate) is essenti

231 Heparanase (HPSE) is the sole mammalian endoglycosidase that degrades heparan sulfate (HS) prote

232 Gs can be cleaved by HPR1 (heparanase-1), an endoglycosidase that is overexpressed in many types of m

233 Hyaluronidase is a hyaluronic acid-degrading endoglycosidase that is present in many toxins and the l

234 GAS secretes EndoS, an endoglycosidase that specifically cleaves the conserved

235 Heparanase-1 (HPR1) is an endoglycosidase that specifically degrades the heparan s

236 Heparanase is an endoglycosidase that specifically degrades the unbranche

237 based proteomics strategy that uses specific endoglycosidases to introduce mass signatures that disti

238 using mass spectrometry in conjunction with endoglycosidase treatment and tryptic digestion.

239 Endoglycosidase treatment demonstrated presence of an in

240 Endoglycosidase treatment of the full-length ANP recepto

241 mutagenesis of the glycosylation site or by endoglycosidase treatment, reduced receptor activation.

242 Tunicamycin and endoglycosidase treatments determined the extent of rNKp

243 Heparanase (HPSE), a host endoglycosidase, when up-regulated by HSV-1 infection di