ライフサイエンスコーパス: endonuclease I

1 iday junctions by T4 endonuclease VII and T7 endonuclease I.

2 or 10-nucleotide flap DNA substrate by Flap Endonuclease I.

3 hanism of the amyloidogenic conversion of T7 endonuclease I.

4 volved in this process is structure-specific endonuclease-I.

5 However, the Mms4-Mus81 endonuclease is 25 times more active on branched duplex

6 structures, suggesting that deoxyinosine 3'-endonuclease is a bacterial functional homologue of huma

7 The tRNA splicing endonuclease is a highly evolutionarily conserved protei

8 The PvuII restriction endonuclease is a homodimer that recognizes and cleaves

9 Apurinic/apyrimidinic endonuclease is a key enzyme in the process of base exci

10 The FokI restriction endonuclease is a monomeric protein that recognizes an a

11 The primary target of SgrAI restriction endonuclease is a multiple sequence of the form 5'-CPu/C

12 side following incision at its 5' side by AP endonuclease is a prerequisite to completion of these re

13 The SfiI endonuclease is a prototype for DNA looping.

14 The SfiI restriction endonuclease is a tetramer in which two subunits form a

15 dual recognition sites 4-6 bp long, the SfiI endonuclease is a tetrameric protein that binds to two c

16 The SfiI endonuclease is a tetrameric protein with two DNA-bindin

17 Bacterial RNA-directed Cas9 endonuclease is a versatile tool for site-specific genom

18 APE1, the major human AP endonuclease, is a druggable target in cancer and its bi

19 p, a member of the RNase III family of dsRNA endonucleases, is a key component of the Saccharomyces c

20 Here we show that T7 endonuclease I, a naturally domain-swapped dimeric prote

21 When the T7 gene 3-encoded DNA debranching endonuclease is absent during in vitro T7 DNA concatemer

22 Internal cleavage by this endonuclease is accelerated by the presence of a monopho

23 owever, the mechanism by which the Mlh1-Mlh3 endonuclease is activated is unknown.

24 tion and chemical crosslinking indicate that endonuclease I also exists in free solution as a dimer t

25 Crenarchaeal XPF, a 3'-flap endonuclease, is also stimulated by PCNA in vitro.

26 The influenza endonuclease is an essential subdomain of the viral RNA

27 The S. pyogenes (Sp) Cas9 endonuclease is an important gene-editing tool.

28 Serratia endonuclease is an important member of a class of magnes

29 The EcoRI endonuclease is an important recombinant DNA tool and a

30 intriguing hypothesis in which the splicing endonuclease is an intermediate in the transition from t

31 This proved that the endonuclease is an intrinsic activity of baculovirus AN.

32 Dicer, an RNase III endonuclease, is an essential component of the microRNA

33 ta show that the form of the complex between endonuclease I and a DNA junction depends on the core of

34 e have constructed a model of the complex of endonuclease I and a DNA junction.

35 complex with a junction-resolving enzyme, T7 endonuclease I, and indeed, one intermediate forms a sta

36 rmined the specificity profile of the homing endonuclease I-AniI and compared it to the conservation

37 dentified homologues of the LAGLIDADG homing endonuclease I-AniI and their putative target insertion

38 The monomeric homing endonuclease I-AniI cleaves with high sequence specifici

39 dditional depletion of apurinic/apyrimidinic endonuclease I (APE1) confers hypersensitivity above tha

40 human uracil-DNA glycosylase 1 (UNG1) and AP endonuclease I (APE1) have optimal activities at specifi

41 myloid-like fibrils in which molecules of T7 endonuclease I are linked by intermolecular disulfide bo

42 We find that ErrASE and T7 Endonuclease I are the most effective at decreasing aver

43 r genome, strand discrimination via the Pms1 endonuclease is as important for MMR as is initial misma

44 eavage activities observed for the native T7 endonuclease I (as distinct from the resolution activity

45 cally evaluated by low throughput assays (T7 endonuclease I assay, target amplification followed by h

46 was confirmed at the genome level with a T7-endonuclease-I assay; protein level with immunocytochemi

47 he continuous strands of a junction bound by endonuclease I, at sites close to (but not identical wit

48 This endonuclease is believed to function in the initial step

49 C1-XPF heterodimer, a structure-specific DNA endonuclease, is best known for its function in the nucl

50 n (H144), consisting of Lac repressor and T7 endonuclease I, binds at the lac operator and cleaves re

51 The endonuclease is blocked by ATP and is thus experimentall

52 Here, we describe a GIY-YIG family homing endonuclease, I-BmoI, that possesses an unusual recognit

53 ate we demonstrate that an active subunit of endonuclease I can act as a junction-specific nuclease i

54 hat the HsdR subunit of a type I restriction endonuclease is capable of independent enzyme activity,

55 Using mass spectrometry we have shown that endonuclease I catalyzes the breakage of the P-O3' bond,

56 RNA interference of structure-specific endonuclease-I caused persistence of a single ribonucleo

57 e III family of double-stranded RNA-specific endonucleases is characterized by the presence of a high

58 In mice, this endonuclease is coded by the Apex gene, disruption of wh

59 The discussion of type II restriction endonucleases is comprised of a description of the gener

60 Finally, the evolution of homing endonucleases is considered, both at the structure-funct

61 ere site-specific targetable activity of Cas endonucleases is coupled with the reverse transcriptase

62 cture of the LAGLIDADG intron-encoded homing endonuclease I-CreI bound to homing site DNA has been de

63 Both heatshock-inducible endonuclease I-CreI expression and X-ray irradiation can

64 es in which the homodimeric LAGLIDADG homing endonuclease I-CreI is altered at individual side-chains

65 etic approach in analyzing individual homing endonuclease I-CreI protein/DNA contacts, and describe h

66 The homing endonuclease I-CreI recognizes and cleaves a particular

67 We used the endonuclease I-CreI to generate acentric chromosomes in

68 ARCUS nucleases, engineered from the homing endonuclease I-CreI, have programmable sequence specific

69 The homing endonuclease, I-CreI, was originally isolated from the c

70 rget sites of the canonical LAGLIDADG homing endonucleases I-CreI and I-MsoI.

71 vidence that integrity of the Integrator RNA endonuclease is critical for brain development.

72 report that P. aeruginosa type I restriction endonuclease is degraded and the methyltransferase is pa

73 ence of the amyloidogenic conformation of T7 endonuclease I depends sensitively on solution condition

74 as also supported by their sensitivity to T7 endonuclease I digestion.

75 ural variant of the CEL I family of mismatch endonucleases is discussed.

76 t AAV, the DNA sequence cleaved by the viral endonuclease is distinct from all other described seroty

77 Ty1ADE2 elements also carried a site for the endonuclease I-DmoI, which we demonstrate is not present

78 fic endonuclease by fusing domains of homing endonucleases I-DmoI and I-CreI and creating a new 1400

79 te, although some restoration of activity in endonuclease I E65D was observed in the presence of Mn2+

80 The I-PpoI endonuclease is encoded by a group I intron found in the

81 The N-terminal region of EcoRI endonuclease is essential for cleavage yet is invisible

82 unit of ERCC1-XPF nucleotide excision repair endonuclease, is essential for meiosis and important for

83 raction before the engineering of new homing endonucleases is essential for further enzyme modificati

84 Digestion of Holliday junctions with T7 endonuclease I establishes that the junction is not trap

85 restricting phage lambda infection when the endonuclease is expressed in E. coli.

86 The yeast HO endonuclease is expressed in late G1 in haploid mother c

87 of one of the DNA replication factors, flap endonuclease I (FEN1), in regulating telomerase activity

88 gmentation by a methyl-sensitive restriction endonuclease is followed by size fractionation and hybri

89 Previously we showed that T7 endonuclease I forms amyloid-like fibrils.

90 We show that T7 endonuclease I forms fibrils at pH 6.8, but not at pH 6.

91 lting covalently linked fibrils show that T7 endonuclease I forms fibrils by a runaway domain swap.

92 igestion of linear DNA by type I restriction endonucleases is generally activated following the head-

93 The cellular concentration of this endonuclease is governed by a feedback mechanism in whic

94 the use of mesophilic pAgos as programmable endonucleases is hampered by their limited action on dou

95 The crystal structure of endonuclease I has been solved both as free protein and

96 he mature mRNA, and thus the activity of the endonuclease is highly regulated.

97 The introns encode the DNA endonucleases I-HmuI and I-HmuII, respectively, which be

98 We have studied the structure-specific endonuclease-I homolog from Trypanosoma brucei, showing

99 The Mus81-Eme1 endonuclease is implicated in the efficient rescue of br

100 homology to the XPF subunit of the ERCC1-XPF endonuclease, is important for replicational stress tole

101 ordinator of multiple DNA structure-specific endonucleases, is important for several DNA repair pathw

102 re of the junction-resolving enzyme phage T7 endonuclease I in complex with a synthetic four-way DNA

103 nterica serovar Paratyphi A, the restriction endonuclease is inactive, apparently due to a mutation i

104 In contrast to the exonuclease, the endonuclease is inhibited completely by 32 protein bindi

105 The human Mus81-Eme1 endonuclease is involved in recombination repair, but th

106 The ERCC1-XPF endonuclease is involved in this unhooking step and in t

107 Endonuclease I is a 149 amino acid protein of bacterioph

108 T7 endonuclease I is a dimeric nuclease that is selective f

109 Endonuclease I is a DNA junction-selective resolving enz

110 Endonuclease I is a junction-resolving enzyme encoded by

111 T7 endonuclease I is a nuclease that is selective for the s

112 Phage-encoded resolvase T7 endonuclease I is a structure-specific endonuclease.

113 Endonuclease I is active in the presence of magnesium, m

114 The junction-resolving enzyme endonuclease I is selective for the structure of the DNA

115 obtain hydroxyl radical footprinting data on endonuclease I-junction complexes that are not complicat

116 age specificity of the intron-encoded homing endonuclease I-MsoI using a physically realistic atomic-

117 f-target analysis was performed using the T7 endonuclease I mutation detection assay and Sanger seque

118 The gene encoding the vsr endonuclease is next to the gene specifying the E. coli

119 r the cleavage reaction, the identity of the endonuclease is not known.

120 Endonuclease I of bacteriophage T7 is a DNA junction-res

121 Endonuclease I of phage T7 is a member of the nuclease s

122 Further analysis shows that this endonuclease is of chimeric origin, incorporating a DNA-

123 Here we track fluorescent dimers of endonuclease I on DNA, presenting the complete single-mo

124 iday junctions, whereas the Mus81-associated endonuclease is one order of magnitude more active upon

125 However, the endonuclease is poorly expressed in the cytosolic fracti

126 e in the specificity enhancement of the Cas9 endonuclease is poorly understood.

127 s of DSBs induced by either radiation or the endonuclease I-PpoI in human somatic cells.

128 The homing endonuclease I-PpoI severely bends its DNA target, resul

129 aracterize variants of the eukaryotic homing endonuclease I-PpoI that were able to bind a mutant, cle

130 comparison to one other nuclease, the homing endonuclease I-PpoI, that has recently been shown, in sp

131 by exploiting the specificity of the homing endonuclease I-PpoI, which is able to selectively cleave

132 of direct protein transduction of the homing endonuclease, I-PpoI, into human cells to generate site-

133 zed cleavage sites for the eukaryotic homing endonucleases I-PpoI and I-CreI were constructed, and si

134 tially corrects C/G transversions whereas T7 Endonuclease I preferentially corrects A/T transversions

135 Remarkably, no homolog of this key endonuclease is present in many bacterial species, such

136 rk forms when the gene 3-encoded debranching endonuclease is present.

137 nism for catalysis of DNA cleavage by I-PpoI endonuclease is proposed and tested by creating six vari

138 The yUtp24/hUTP24 PIN endonuclease is proposed to cleave at sites A1/1 and A2/

139 inding and cleavage by the SgrAI restriction endonuclease is proposed.

140 RNase III, a double-stranded RNA-specific endonuclease, is proposed to be one of Escherichia coli'

141 and DNA repair enzyme apurinic/apyrimidinic endonuclease I protect smooth muscle cells against oxida

142 in Saccharomyces cerevisiae, encoding the HO endonuclease, is regulated by two distinct PUF proteins,

143 property of the exonuclease III family of AP endonucleases is remarkably conserved from Archaea to hu

144 Our results also suggest that the Ercc1/Xpf endonuclease is required for efficient removal of non-ho

145 ERCC1-XPF endonuclease is required for nucleotide excision repair

146 The Fan1 endonuclease is required for repair of DNA interstrand c

147 The nucleolytic activity of MUS81 endonuclease is required for replication fork restart un

148 strand crosslink (ICL) repair, the XPF-ERCC1 endonuclease is required for the incisions that release,

149 Further, the Apn2 endonuclease is required for the viability of smc6 mutan

150 The Rad1/Rad10 endonuclease is required to trim intermediates formed du

151 The endonuclease is resistant to RNase A inhibitors, sensiti

152 Digestion of the dHJ substrate by T7 endonuclease I resolves the substrate into crossover and

153 The SP82-encoded endonuclease is responsible for exclusion of the SPO1 in

154 In yeast, the tRNA splicing endonuclease is responsible for identification and cleav

155 The RNA splicing endonuclease is responsible for recognition and excision

156 tails after treatment with the rare-cutting endonuclease I-SCE:I.

157 initiated by DSBs induced by a rare cutting endonuclease (I-SceI) into one of two direct DNA repeats

158 an 18-base pair recognition site of a homing endonuclease (I-SceI), which is found by chance only onc

159 of two tandem noncohesive DSBs generated by endonuclease I-SceI and the 3' nonprocessive exonuclease

160 rate containing a recognition site for yeast endonuclease I-SceI embedded within a functional tk gene

161 rate containing a recognition site for yeast endonuclease I-SceI embedded within a functional tk gene

162 A genomic DSB was induced by introducing endonuclease I-SceI into cells containing a stably integ

163 romosomal double-strand break created by the endonuclease I-SceI or multiple breaks created by treatm

164 system is based on the stable integration of endonuclease I-SceI recognition sites flanked by bacteri

165 This method uses the mitochondrial endonuclease I-SceI that recognizes and cuts an 18-bp re

166 hen electroporated with a plasmid expressing endonuclease I-SceI to induce a DSB, and clones that had

167 The same result was obtained when the homing endonuclease I-SceI was used to initiate HDR at the targ

168 that combines the specificity of the homing endonuclease I-SceI with the type IIS cleavage pattern o

169 ere electroporated with a plasmid expressing endonuclease I-SceI, and clones that had lost tk functio

170 ining a cleavage site for the intron-encoded endonuclease I-SceI, which is not otherwise found in the

171 le strand break introduced by a rare cutting endonuclease I-SceI.

172 pted by the 18-bp recognition site for yeast endonuclease I-SceI.

173 ne, or no genomic sites for the rare-cutting endonuclease I-SceI.

174 de containing the 18-bp recognition site for endonuclease I-SceI.

175 Bacteriophage T7 endonuclease I selectively binds and cleaves DNA four-wa

176 ined metastable conformational states within endonuclease I, showing how these states can be influenc

177 Unlike most ribonucleases, this viral endonuclease is shown to catalyze the hydrolysis of the

178 L1 endonuclease is specific for the unusual DNA structural

179 The homing endonuclease I-Ssp6803I causes the insertion of a group

180 inding and cleavage by the PD-(D/E)XK homing endonuclease I-Ssp6803I.

181 his intronic ORF encodes a double-strand DNA endonuclease, I-Ssp6803I.

182 that the N-terminal two-thirds of the I-CreI endonuclease is sufficient for homing site recognition a

183 ed into cells, suggesting that any apoptotic endonuclease is sufficient to induce gamma-H2AX formatio

184 targeted, specific DNA invasion and used T7 endonuclease I (T7EI) to recognize and cleave the gammaP

185 ion between protein and enzyme activity, the endonuclease is tentatively identified as a approximatel

186 After the td intron endonuclease I-TevI cleaves the intronless recipient 23

187 volution experiments with the GIY-YIG homing endonuclease I-TevI that targets the thymidylate synthas

188 td intron is initiated by the intron-encoded endonuclease I-TevI, which cleaves the intronless allele

189 ce of the phage T4 td group I intron and its endonuclease, I-TevI, for which the distance separating

190 The phage T4 td intron endonuclease, I-TevI, is responsible for this infidelity

191 We have also constructed a mutant of endonuclease I that lacks nine amino acids (six of which

192 The CRISPR-Cas-derived RNA-guided Cas9 endonuclease is the key element of an emerging promising

193 The nuclear localization of this endonuclease is the unique feature that distinguishes it

194 EcoRV endonuclease is thus not a "new paradigm" for site-speci

195 nts to analyze induced fit in the binding of endonuclease I to a DNA four-way junction.

196 ude that the function of the retrotransposon endonucleases is to define and cleave target site DNA.

197 the Hol75 DNA by T4 endonuclease VII and T7 endonuclease I, two enzymes known to cleave such junctio

198 Intron 2 encodes a DNA endonuclease, I-TwoI, with similarity to homing endonucl

199 The BisI family of restriction endonucleases is unique in requiring multiple methylated

200 eature between an intronic and free-standing endonuclease is unprecedented.

201 I-TevI, the T4 td intron-encoded GIY-YIG endonuclease, is very similar to I-BmoI, but each endonu

202 orrectly folded catalytic domain (SCD) of T7 endonuclease I was produced by means of a trans-splicing

203 phosphorylation sites, recombinant human AP endonuclease is weakly phosphorylated in vitro (4% at be

204 cture of the DNA bound in the active site of endonuclease I, where the proS oxygen atom is coordinate

205 in other tissues, suggesting that the repair endonuclease is widely utilized for repair of DNA damage

206 We have solved the crystal structure of endonuclease I with a wild-type active site.