ライフサイエンスコーパス: endonuclease IV

1 ale for the modulation of the recognition by endonuclease IV.

2 bed exonuclease activity of Escherichia coli endonuclease IV.

3 ely 2-fold higher by the addition of E. coli endonuclease IV.

4 f the 3'-phosphate processing enzyme E. coli endonuclease IV.

5 that contains uracil N-glycosylase (UNG) and endonuclease IV.

6 th 32% sequence identity to Escherichia coli endonuclease IV.

7 atment of E. coli uracil-DNA glycosylase and endonuclease IV.

8 a coli AP endonucleases, exonuclease III and endonuclease IV.

9 lates inversely with the in vivo activity of endonuclease IV.

10 e including the essential AP endonuclease TK endonuclease IV.

11 cts, bulges, abasic sites and the binding of endonuclease IV.

12 ng: (1) 5'-DNA strand incision of the DPC by endonuclease IV; (2 to 4) strand-displacement DNA synthe

13 However, in vitro treatment with purified endonuclease IV activated subsequent DNA synthesis with

14 Oxidized abasic site repair by endonuclease IV and endonuclease III (C4-AP only) is app

15 The crystal structures of Endonuclease IV and its AP-DNA complex at 1.02 and 1.55

16 By combining the activity of endonuclease IV and T4 endonuclease V on highly purified

17 condary structure similar to that of E. coli endonuclease IV and that the T. maritima endonuclease IV

18 A sequence was incised by exonuclease III or endonuclease IV approximately 6-fold more efficiently th

19 Exonuclease III and endonuclease IV are the major enzymes in E. coli respons

20 i 3'-phosphodiesterases, exonuclease III and endonuclease IV, are readily detected in crude cell extr

21 scherichia coli enzymes, exonuclease III and endonuclease IV, are used.

22 Endonuclease IV belongs to a class of important apurinic

23 Apn1 is related to Echerichia coli endonuclease IV, both in its enzymatic properties and it

24 vity at the positions of exonuclease III and endonuclease IV but retain activity in the position of a

25 ase IV structure is more stable than E. coli endonuclease IV by almost 20 degrees C, beginning to rap

26 ves the uracil base from the nucleotide, and endonuclease IV cleaves the phosphodiester bond at this

27 Repair in the absence of endonuclease IV could be attributed to hydrolysis of the

28 er a 1 h bleomycin treatment from wild-type, endonuclease IV-deficient (nfo-) and endonuclease IV-ove

29 lase-deficient, ung-, or exonuclease III and endonuclease IV-deficient, xth-nfo-) and luciferase acti

30 ining 8-oxoG opposite a single strand break, endonuclease IV DNA polymerase I and Escherichia coli DN

31 of Fpg, repair of the single strand break by endonuclease IV, DNA polymerase I and DNA ligase occurre

32 Here we describe a novel endonuclease IV (Endo IV) based assay utilizing a substr

33 he 5'-terminal deoxyribose phosphate from an endonuclease IV (endo IV) pre-incised AP site.

34 The ability of endonuclease IV (Endo IV) to efficiently incise alpha-de

35 Endonuclease IV (Endo IV), a member of the base excision

36 The effect of the AP endonucleases endonuclease IV (Endo IV), exonuclease III (Exo III), an

37 with the Escherichia coli DNA repair enzyme endonuclease IV (endo IV), which recognizes apurinic/apy

38 ic activities that are characteristic of the endonuclease IV family of DNA repair enzymes, including

39 ed by digestion of plasmid DNA with apurinic endonuclease IV, followed by primer extension and/or PCR

40 The T. maritima endonuclease IV gene encodes a 287-amino-acid protein wi

41 oism spectroscopy indicates that T. maritima endonuclease IV has secondary structure similar to that

42 An endonuclease IV homolog was identified as the product of

43 R requires Apn1 protein, an Escherichia coli endonuclease IV homolog.

44 nce of a substrate, suggesting that it is an endonuclease IV homologue possessing intrinsic metal cof

45 Endonuclease IV incision efficiency of 2-deoxyribonolact

46 dideoxynucleoside triphosphates lowered the endonuclease IV-independent priming activity, but did no

47 Endonuclease IV is the archetype for a conserved apurini

48 Similarly, endonuclease IV (Nfo) does not incise L or F when they a

49 es associated with exonuclease III (xth) and endonuclease IV (nfo), indicating for the first time tha

50 Endonuclease IV of Escherichia coli has been implicated

51 imulation was observed with Escherichia coli endonuclease IV or an N-terminal truncated APE1 fragment

52 pecialized 3'-end processing enzymes such as endonuclease IV or exonuclease III are not absolutely re

53 d-type, endonuclease IV-deficient (nfo-) and endonuclease IV-overproducing (p-nfo; approximately 10-f

54 We further demonstrated that endonuclease IV plays a major role in incising an AP-pep

55 ved by Escherichia coli endonuclease III and endonuclease IV (prototype AP endonucleases) and S.POMBE

56 ia coli Fpg protein and E. coli Nfo protein (endonuclease IV), respectively, as well as double-strand

57 Thus, endonuclease IV-specific damage can be detected after in

58 .coli DNA polymerase I, to determine whether endonuclease IV-specific damage could be detected in the

59 Addition of E. coli endonuclease IV stimulated Dug activity by enhancing the

60 oli endonuclease IV and that the T. maritima endonuclease IV structure is more stable than E. coli en

61 ised less efficiently by exonuclease III and endonuclease IV than are other abasic lesions.

62 t affect the amount of activation seen after endonuclease IV treatment.

63 f the reaction the trinuclear active site of endonuclease IV underwent dramatic local conformational

64 a quantitative comparison demonstrated that endonuclease IV was > or = 5-fold more active in this as

65 Catalytically active endonuclease IV was apparently required to mediate Dug t

66 f L and C4-AP lesions by exonuclease III and endonuclease IV was determined under steady-state condit

67 lease shows a striking similarity to E. coli endonuclease IV, which provides clues regarding the mech