ライフサイエンスコーパス: enteroid

1 ian clock in 3D murine intestinal organoids (enteroids).

2 n Mtgr1(-/-) whole intestines and Mtgr1(-/-) enteroids.

3 pattern was also observed in differentiated enteroids.

4 response to cAMP and elevated calcium in T1D enteroids.

5 the bacterium to infect 3D bovine basal-out enteroids.

6 ently and inhibited GII.3 cellular uptake in enteroids.

7 tivation in vivo and in both mouse and human enteroids.

8 riants with high potency in human intestinal enteroids.

9 ary human airway cells and stem cell-derived enteroids.

10 n cell lines, nonhuman cell lines, and human enteroids.

11 nted HAstV infection in cell lines and human enteroids.

12 ifferentiation and maturation versus healthy enteroids.

13 of BA transporters was attenuated in NT(-/-) enteroids.

14 uration, and longevity of tubular intestinal enteroids.

15 re rhythmically expressed in mouse and human enteroids.

16 a median phase shift of 6.2 hours in murine enteroids.

17 nthetic lethality in HIF-2a-expressing tumor enteroids.

18 out cell differentiation in human intestinal enteroids.

19 man intestinal epithelial cell monolayer and enteroids.

20 of the BA-dependent strain GII.3 in jejunal enteroids.

21 functional Na+/H+ exchange activity in MVID enteroids.

22 hogenesis using human colonoids and duodenal enteroids.

23 UROG3 protein levels in BON4 cells and human enteroids.

24 d enhanced CFTR-dependent fluid secretion in enteroids.

25 control mice, and established 3-dimensional enteroids.

26 inking the circadian clock and cell cycle in enteroids.

27 observed in cell lines and human intestinal enteroids.

28 ition in undifferentiated and differentiated enteroids.

29 KO of Fzd5, not Fzd8, in enteroids ablated responsiveness to dual-specificity FZD

30 < .05) and conditional knockout of HDAC3 in enteroids abrogated butyrate effects on Per2 expression.

31 In primary 3-dimensional enteroids, administration of excess Cu or Cu chelators i

32 eta-catenin target gene expression in murine enteroids and colonoid cultures and TNF-induced beta-cat

33 encing (RNA-seq) of metabolite-exposed human enteroids and duodenal biopsies post-FMT confirmed trans

34 embryonic fibroblasts from mice, along with enteroids and human IEC lines, we found that induction o

35 on utilization of three-dimensional primary enteroids and organoids for mechanistic studies of intes

36 Using untransformed ex situ human intestinal enteroids and transformed Caco-2 cells, we report that E

37 fferocytosis, ex vivo intestinal organoids ("enteroids"), and in vivo Cre-mediated deletion of Paneth

38 Here, we use human and mouse enteroids, and a mouse model of pathogenesis, to explore

39 inactivated by TcdB, between mouse and human enteroids, and disruption of Rac1 abolishes clock-depend

40 RNA expression were assessed in ileum, mouse enteroids, and human cell lines.

41 elopment and maintenance of upper intestinal enteroids, and these effects were rescued by acetate tre

42 milar in undifferentiated and differentiated enteroids, and was affected by known inhibitors, second

43 Biopsy-derived human intestinal enteroids are a biomimetic of the intestinal epithelium

44 Human intestinal crypt-derived enteroids are a model of intestinal ion transport that r

45 Enteroids are a physiologically relevant model to examin

46 When enteroids are cocultured with CD90(+) mesenteric lymph n

47 is very limited but increases ~10-fold when enteroids are differentiated to include M cells.

48 Enteroids are three-dimensional structures of primary sm

49 Our data supports the use of pediatric enteroids as an ex-vivo model to advance studies of GI c

50 y askew in human small intestinal organoids (enteroids) as compared to that in paired tissue from whi

51 Utilizing primary human duodenal organoids (enteroids) as in vitro models of the functional GI epith

52 ions, efficient phagocytosis, and stabilized enteroid barrier function revealed a coordinated respons

53 ced the expression of alpha-defensin mRNA in enteroids by >100,000-fold, restoring DEFA5 and DEFA6 to

54 ndritic cells, B cells and stem-cell-derived enteroids can all support infection of certain norovirus

55 While the enteroid cell culture system became widely used in HuNoV

56 s using an expanded repertoire of intestinal enteroid cell lines.

57 and characterized a primary human macrophage-enteroid co-culture model for in-depth studies of epithe

58 tablished the first primary human macrophage-enteroid co-culture system, defined conditions that allo

59 eltaLyz) ) mouse model and used a macrophage/enteroids coculture system to examine tissue-specific VD

60 were reproducible in human Bmal1-luciferase enteroids, colonoids, and Per2-luciferase Caco-2 cells.

61 ated intestinal crypts or stem cells (termed enteroids/colonoids) and from inducible pluripotent stem

62 In mouse and human normal and tumor-derived enteroids/colonoids, those that expressed SPDEF for 3 da

63 surement of kinetically labile iron pools in enteroids competent or blocked for iron efflux indicated

64 Live imaging of Myo5b KO-derived enteroids confirmed the formation of inclusions from the

65 Modifications to enteroid culture conditions facilitated robust human cyt

66 Intestinal enteroid culture has enabled long-term propagation of un

67 This new human enteroid culture methodology refines the ability to stud

68 developed-the B cell culture system and the enteroid culture system.

69 d showed it neutralizes noroviruses in human enteroid cultures and abrogates or reduces infection in

70 re formed in HuNoV-infected human intestinal enteroid cultures and are the sites for genome replicati

71 baseline, WNV poorly infects human and mouse enteroid cultures and enterocytes in mice.

72 We infected nontransformed human intestinal enteroid cultures from multiple individuals with human r

73 o measure norovirus inhibition in intestinal enteroid cultures in vitro.

74 Intestinal enteroid cultures provide a relevant system to evaluate

75 colonic epithelial cells and planar colonic enteroid cultures showed a robust secretory response fol

76 In developing intestine enteroid cultures, NRG1, but not EGF, permitted increase

77 acid (BA) to infect human jejunal intestinal enteroid cultures.

78 rticles (VLPs) and modified human intestinal enteroid cultures.

79 human intestinal tissue-derived organoid (or enteroid) cultures, advances in structural biology techn

80 Intriguingly, mouse and human enteroids demonstrate anti-phasic necrotic cell death re

81 Ex vivo culture of PCBP1-depleted enteroids demonstrated no defects in hepcidin-mediated f

82 Enteroids derived from control and ATP7B-knockout mice w

83 ing and replicating intracellularly in human enteroids derived from different segments of the intesti

84 Enteroids derived from IDO1-TG intestine had increased m

85 Human intestinal enteroids derived from intestinal stem cells allow the s

86 Using intestinal organoids (colonoids and enteroids) derived from PWH and controls, we assessed th

87 onella infection, by using murine epithelial enteroid-derived monolayers (EDMs).

88 Salmonella infection of murine enteroid-derived monolayers decreased DRA expression.

89 c biopsy specimens, primary cells, and human enteroid-derived monolayers from healthy human stomach,

90 T(84) cells and murine enteroid-derived monolayers were employed to assess HG e

91 the receptor, both in T(84) cells and murine enteroid-derived monolayers.

92 Enteroids differentiated upon withdrawal of WNT3A, yield

93 Studies in NCI-H716 cells and human jejunal enteroids engineered to make more enteroendocrine cells

94 ed studies with SKCO-15 and human intestinal enteroids established from biopsies from different intes

95 Enteroids established from pediatric T1D duodenum identi

96 However, in vitro, the knockout enteroids exhibit significantly weakened growth that can

97 MVID enteroids exhibited altered differentiation and maturati

98 regulation of alpha-defensins, yet in human enteroids exogenous stimulation of WNT signaling appears

99 pharmacological inhibition of CFTR abrogated enteroid fluid secretion, providing proof of concept for

100 Importantly, DNAJB9 deficiency upregulated enteroids' fluid secretion in CF mice (homozygous for De

101 mucin glycoprotein Muc2 were observed in the enteroids following S. flexneri infection.

102 s demonstrate the utility of patient-derived enteroids for developing therapeutic approaches to MVID.

103 In vitro IGF1 enhanced enteroid formation by Sox9-EGFP(High) facultative ISCs b

104 icantly higher LGR5 expression (p < .01) and enteroid forming efficiency (p < .0001) compared with LG

105 patients and compared them with aged-matched enteroids from healthy subjects (HS) using bulk RNA sequ

106 ocked fluid secretion in primary cultures of enteroids from human small intestine and anion current i

107 Here, we used stem cell-derived enteroids from human small intestines to study enterovir

108 f the intestinal epithelium but establishing enteroids from individuals with long-standing T1D has pr

109 Intestinal tissues and derived enteroids from MYO5B(P663L) piglets had reduced apical l

110 However, intestinal tissues and derived enteroids from MYO5B(P663L) piglets maintained CFTR on a

111 cretion by group 2 innate lymphoid cells and enteroid gene expression consistent with goblet cell dif

112 ock-controlled genes in both mouse and human enteroids governing organism-specific, circadian phase-d

113 The emphasis is on studies with human enteroids grown either as three-dimensional spheroids or

114 cture, ASM induced a significant decrease of enteroid growth without affecting their number.

115 vitro, cultured ErbB4(-/-) ileal epithelial enteroids had reduced Paneth cell markers and were highl

116 urable cytokine response of human intestinal enteroids has been limited following exposure to host or

117 lopment of animal models and patient-derived enteroids, has provided critical insights into the enter

118 Human enteroids have emerged as a physiologically relevant mod

119 Since the development of human intestinal enteroid (HIE) cell culture systems, significant advance

120 rovirus replication using a human intestinal enteroid (HIE) culture system and/or could block norovir

121 Human intestinal enteroid (HIE) cultures contain multiple intestinal epit

122 CRISPR-Cas9 modification of human intestinal enteroid (HIE) cultures, and functional studies with two

123 emiology and infectivity in human intestinal enteroid (HIE) monolayers and clinical characteristics o

124 The development of the human intestinal enteroid (HIE) system has revolutionized norovirus resea

125 infection in biopsy-derived human intestinal enteroids (HIE) has been described: 3D-HIE are first dis

126 Human intestinal enteroids (HIEs) are primary epithelial cell cultures th

127 ey [P16] HuNoV strain using human intestinal enteroids (HIEs) from adults and children.

128 However, human intestinal enteroids (HIEs) have emerged to offer unique ex vivo mo

129 ion of two HuSaV strains in human intestinal enteroids (HIEs) known to support the replication of HuN

130 fected cells and DGAT1(-/-) human intestinal enteroids (HIEs) show a decrease in expression of nutrie

131 we generated cell lines and human intestinal enteroids (HIEs) stably expressing cytosolic and/or ER-t

132 Here, using nontransformed human jejunal enteroids (HIEs) that recapitulate the physiology of the

133 w enable the cultivation of human intestinal enteroids (HIEs), which are derived from human intestina

134 the replication of HuSaV in human intestinal enteroids (HIEs), which are nontransformed cultures orig

135 ce factors and adherence to human intestinal enteroids (HIEs).

136 Human intestinal tissue-derived enteroids (HIEs; also called organoids) are a powerful e

137 ve to epithelial cytokine responses by human enteroids, HIEs can facilitate exploration of many exper

138 Murine enteroids, however, recapitulate the expression profile

139 induce goblet cell differentiation in murine enteroids; however, IL-13, a cytokine induced by IL-33,

140 es and 55 (49 stool and six human intestinal enteroids) HuNoV positive samples encompassing major sub

141 L-33-induced Muc2 expression is dependent on enteroid Il4ra expression, demonstrating a requirement f

142 We developed an enteroid-immune cell coculture model to determine the me

143 We cultured enteroids in media supplemented with sterile supernatant

144 4/Sydney/2012-like virus in human intestinal enteroids in samples collected before and 4 weeks after

145 also highlights the use of human intestinal enteroids in the study of enteric diseases.

146 e, we reveal that mouse and human intestinal enteroids in three-dimensional ex vivo cultures express

147 ntestinal crypts from C57BL/6 mice, cultured enteroids, incubated these with TNF (50 ng/mL, 24 hours)

148 RNA sequence (RNA-seq) analysis of human enteroids indicated that the effects of 1,25(OH)(2)D(3)

149 ary to an immortalized intestinal cell line, enteroids induced antiviral and inflammatory signaling p

150 Enteroid (intestinal epithelial organoid)-based studies

151 denosine monophosphate with forskolin caused enteroid intracellular acidification in HCO3(-)-free buf

152 Cyclic adenosine monophosphate-induced enteroid intracellular pH acidification as part of duode

153 ation quantified simultaneously in scores of enteroid lumens, recapitulating ETEC-induced intestinal

154 The human enteroid model promises to bridge some of the gaps betwe

155 The duodenal enteroid model showed that electrogenic Na(+)/HCO3(-) co

156 The human intestinal enteroid model, derived from LGR5(+) stem cell-containin

157 a physiologically relevant human intestinal enteroid model.

158 Use of enteroid models revealed ASM and ceramide-mediated delet

159 In mouse and human enteroid models, NHERF4 peptides dose-dependently reduce

160 , both adhesins contribute to adhesion in an enteroid monolayer colonization model.

161 ell-derived, nontransformed human intestinal enteroid monolayer cultures.

162 ing a high-throughput approach that combines enteroid monolayers and quantitative imaging, we identif

163 es enhanced barrier function and maturity of enteroid monolayers as indicated by increased transepith

164 Human intestinal stem cell-derived enteroid monolayers co-cultured with human monocyte-deri

165 ctions with human intestinal epithelia using enteroid monolayers on permeable supports (such as Trans

166 IEC (Caco-2BBe/HT-29.cl19a cells) and mouse enteroid monolayers were cocultured with human macrophag

167 bility in cocultures with human IEC or mouse enteroid monolayers, which were restored by anti-IL6.

168 m human small intestine and anion current in enteroid monolayers.

169 Intestinal enteroids offer a unique model, integrating the GUCY2C s

170 to mice and patient-derived human intestinal enteroids possess circadian rhythms and demonstrate circ

171 Further, Kaiso knockdown in intestinal enteroids reduced stem- and WNT-associated phenotypes, t

172 Human patient-derived enteroids represent a model for investigation of monogen

173 ke, and differentiated or villus-like, human enteroids represent distinct points along the crypt-vill

174 neutralization assay using human intestinal enteroids reveal that the GII-specific mAb NORO-320, med

175 Enteroid secretion depended on canonical molecular signa

176 Moreover, Gal9(-/-)-derived enteroids showed impaired growth ex vivo.

177 The shp85alpha enteroids showed increased mRNA expression of Wnt target

178 The p85alpha-deficient enteroids showed reduced HES1 mRNA and increases in secr

179 DeltaIEC)) mice and p85alpha-deficient human enteroids (shp85alpha).

180 gfbeta signaling in cultured mouse and human enteroids supports further the in vivo data and reveals

181 d HuNoV infectivity via the human intestinal enteroid system and HuNoV persistence via reverse transc

182 robust alternative model of infection in 3D enteroids that undergoes differentiation and spontaneous

183 in epithelial cell lines and murine colonic enteroids through mechanisms possibly involving histone

184 ly the same methods to patient-derived ileal enteroids to explore the utility of using this experimen

185 We used human small intestinal enteroids to study neutral Na(+) absorption and stimulat

186 r intracellular pH and luminal dilatation in enteroids under basal and regulated conditions.

187 We successfully established duodenal enteroids using endoscopic biopsies from pediatric T1D p

188 In vitro cleavage assays and studies of enteroids verified that HMGB1 protects beclin 1 and ATG5

189 knockdown gene and miRNA expression ex vivo enteroids, we demonstrate that we can knock down gene ex

190 Using transgenic mice and cultured enteroids, we now demonstrate that TLR4 induces ER stres

191 formed in human cell lines and human-derived enteroids, we show that IL-22 signaling regulates expres

192 formed in human cell lines and human-derived enteroids, we show that IL-22 signaling regulates expres

193 m tissues were collected from mice; IECs and enteroids were cultured and analyzed by histology, immun

194 thogenic intestinal bacterium, human jejunal enteroids were cultured as monolayers in microengineered

195 Enteroids were generated from pigs with MYO5B(P663L) and

196 Enteroids were induced to differentiation after incubati

197 Crypts were isolated, enteroids were propagated in culture, induced to undergo

198 Recently, human intestinal enteroids were shown to be permissive for norovirus infe

199 3D bovine gut enteroids were susceptible to infection by all NSP3 muta

200 We found that enteroids were susceptible to infection by diverse enter

201 h to engineer vascularized, perfusable human enteroids, which can be used to model innate immune resp

202 We developed human enteroids with different loss-of function MYO5B variants

203 Treatment of intestinal explants and enteroids with Dyngo resulted in accumulation of inclusi

204 +/H+ exchange (NHE) activity in MVID patient enteroids with near-normal chloride secretion.

205 Challenge of Caco2 cells and enteroids with proinflammatory cytokines as well as dext

206 mice with TNF or incubation of crypt-derived enteroids with TNF reduced their expression of DRA messe

207 Coculture of enteroids with VDR(-/-) macrophages increased the deloca

208 crine, and tuft cells, compared with control enteroids, with a concomitant decrease in markers of abs

209 duced the number of intestinal crypt-forming enteroids without affecting their structure, ASM induced

210 affected by the presence of fungi in jejunal enteroids, yet the immune landscape in white adipose tis