ライフサイエンスコーパス: env gene

1 RF13_cpx, each of which contains a subtype A env gene.

2 probes complementary to the V1 region of the env gene.

3 s mediated by the gp120 coding region of the env gene.

4 ial sequence of the gp41 region of the HIV-1 env gene.

5 cine using a deduced ancestral state for the env gene.

6 mbination event may have occurred within the env gene.

7 tution (S84A) in the VRA region of the viral env gene.

8 cates that interferon resistance maps to the env gene.

9 des 752 to 2818 which encompasses the entire env gene.

10 es, and an internal promoter (IP) within the env gene.

11 murine B cells transfected with the HERV-K18 env gene.

12 of soluble trimers based on the clade B B41 env gene.

13 ines revealed second-site changes within the env gene.

14 HIV-gpt proviruses, which lack a functional env gene.

15 ion of the transmembrane (TM) protein of the env gene.

16 resence of unique sequences within the viral env gene.

17 utant containing a point mutation within the env gene.

18 ions and cells transfected with the complete env gene.

19 ent paradigm is based on the BG505 subtype A env gene.

20 ve-like SOSIP.664 trimers based on the BG505 env gene.

21 ions, and recombination events involving the env gene.

22 d polymerase chain reaction specific for the env gene.

23 mutation or recombination of the subgroup A env gene.

24 emerged by mutation or recombination of the env gene.

25 less stringent negative selection within the env gene.

26 SIV(mac251) (ALVAC-SIV/CD40L) gag, pol, and env genes.

27 transmembrane domain, similar to retroviral env genes.

28 d PI and TCLA viruses and molecularly cloned env genes.

29 make recombinant trimers from many different env genes.

30 ed by DNA sequence analysis of pol, gag, and env genes.

31 al fluid (CSF)-derived, R5 macrophage-tropic env genes.

32 t-generation sequencing of the viral gag and env genes.

33 most of them directed against the envelope (env) gene.

34 pression of an interfering MCF MLV envelope (Env) gene.

35 d to be as high as that reported for gag and env genes (13-17%).

36 e sheep retrovirus (JSRV) encodes within the env gene a trans-acting factor (Rej) necessary for the s

37 gammaretroviruses with concentration in the env gene across the GALV strains that were particularly

38 ssing retroviral gag-pol (AdGagPol) and 10A1 env genes (Ad10A1).

39 icate that a vaccine expressing a mismatched Env gene alone can prevent SIV infection in NHPs and ide

40 encoding a murine leukemia virus amphotropic env gene and a green fluorescent protein (GFP) reporter

41 d a large pool of insertion mutations in the env gene and analyzed the fitness of each mutant in comp

42 and tissue tropisms controlled by the HIV-1 env gene and for the analysis of mechanisms of viral imm

43 dependent on sequences at the 3' end of the env gene and not on the polytropic regions or on the 585

44 e evolution of the C2-V5 region of the HIV-1 env gene and of T-cell subsets in nine men with a modera

45 ctivation element [CTE]) located between the env gene and the 3' long terminal repeat of simian retro

46 ptible, full-length, biologically functional env genes and characterized viruses pseudotyped with the

47 human immunodeficiency virus type 1 (HIV-1) env genes and exhibit distinct phenotypes.

48 We transfected various combinations of these Env genes and investigated Env-mediated cell fusion and

49 eficiency virus type 1 (HIV-1) pol, vif, and env genes and the 3' long terminal repeat (LTR) sequence

50 a sequence was also found in analysis of the env gene, and linkage by long-distance reverse transcrip

51 minor HIV-1 variants in the V3 region of the env gene, and to identify minor drug-resistant variants

52 ced by a ribozyme directed against the HIV-1 env gene, and whether the antiviral activity was affecte

53 inant multimeric proteins based on the HIV-1 env gene are current candidate immunogens for vaccine tr

54 Recombinant trimeric proteins based on HIV-1 env genes are being developed for future vaccine trials

55 Recombinant trimeric proteins based on HIV-1 env genes are being developed for vaccine trials in huma

56 Thus, the computer-generated "consensus" env genes are capable of expressing envelope glycoprotei

57 be an experimental approach to analyze HIV-1 env genes as intact genetic units amplified from plasma

58 ct open reading frames for the gag, pol, and env genes, as well as nearly identical flanking long ter

59 ew SOSIP.664 trimer derived from a subtype B env gene, B41, including how to make this protein in low

60 mpared the pattern of evolution of the HIV-1 env gene between individuals with recent HIV infection w

61 V-A, PERV-B, and PERV-C) which have distinct env genes but have highly homologous sequences in the re

62 on values were similar for the gag, pol, and env genes but these genes differed significantly from th

63 mapped to specific regions in the envelope (env) gene by using a cloning-hybridization technique and

64 one, HIV-Du151.2env-NLuc, which expresses an env gene (C.Du151.2) cloned from an acute heterosexually

65 t, substitution of both the FeLV-945 LTR and env gene changed the disease outcome entirely.

66 ess of the primary B5 HIV-1 isolates and its env gene cloned into the NL4-3 laboratory strain had sim

67 able to that seen with several primary HIV-1 env genes cloned from individuals with disease progressi

68 Primary env genes cloned from sequential isolates from two seroc

69 ested and was exclusively R5 tropic, and its env gene clustered with HIV-C by phylogenetic analysis;

70 synthesized an artificial group M consensus env gene (CON6 gene) to be equidistant from contemporary

71 have generated a synthetic group M consensus env gene (CON6) for induction of cross-subtype immune re

72 the numerous copies of endogenous retroviral env genes conserved within mammalian genomes act as rest

73 and gp41 was demonstrated in that a chimeric env gene consisting of a gp120 coding sequence from an N

74 All three env genes contained 3' regions identical to that of SFFV

75 n resulting in acquisition of a heterologous env gene could in theory facilitate cross-species transm

76 ent study, we investigated whether envelope (env) genes could be amplified from proviral DNA or RNA d

77 constructing mutants with either the pol or env gene deleted.

78 Cloning of this endogenous retroviral env gene demonstrated fusogenicity in an ex vivo cell-ce

79 he HERV-W env gene represents the first HERV env gene demonstrated to encode the functional propertie

80 enesis of multiple primary and prototypic R5 env genes demonstrated that the GPG motif was necessary

81 vaccination with a mismatched SIV envelope (Env) gene, derived from simian immunodeficiency virus SI

82 antiretroviral env, we argue that many more env gene-derived restriction factors await discovery in

83 leukosis virus (ALV) that includes a unique env gene, designated J, was identified recently in Engla

84 with selective deletion of the gag, pol, and env genes developed extensive vasculopathy.

85 To determine if changes in env gene diversification occur with NNRTI therapy, we us

86 sign is designated SOSIP.664, but many HIV-1 env genes do not yield fully native-like trimers efficie

87 -tropic proteins, while the remaining cloned env genes encoded R5 T cell-tropic proteins.

88 The Friend spleen focus-forming virus (SFFV) env gene encodes a 409-amino-acid glycoprotein with an a

89 The Friend spleen focus-forming virus (SFFV) env gene encodes a glycoprotein with apparent Mr of 55,0

90 The env gene encodes a protein that is over 40% identical in

91 al might be an artifact of the region of the env gene evaluated and that regions other than V3 might

92 Viral env genes evolving from individual transmitted or founde

93 unodeficiency virus type 1 (HIV-1) envelope (env) gene exhibits a high level of genetic heterogeneity

94 ormation following transfection with primary env gene expression vectors.

95 To study the properties of these env genes, expression plasmids for the three env genes w

96 erythropoietin receptor (EpoR) by the virus env gene (F-gp55), aberrant over-expression of the trans

97 ino acids of the N-terminal mature SU of FRA env gene, followed by eight amino acids from the framesh

98 n be adapted for use with multiple different env genes for both vaccine and structural studies.

99 ve paired blood-derived, T cell-tropic HIV-1 env genes, four of which are CCR5-using (R5) and one of

100 -R) activation, such as interaction with the env gene Friend virus envelope glycoprotein (F-gp55) of

101 ction with a eukaryotic vector expressing an env gene from a cell culture-adapted strain of virus cor

102 n with HIV-1 env DNA plus HIV-1 Env protein (env gene from HXBc2 clone of HIV IIIB; Env protein from

103 In the analysis of full env genes from 22 early seroconverters, the Trinidad iso

104 uencing (UDS) of partial HIV-1 gag, pol, and env genes from 32 recently infected individuals.

105 inant vaccinia vectors (vv) expressing HIV-1 env genes from early isolates of four vertically infecte

106 gh frequency of G-to-A hypermutations in the env genes from HIV-1BaL-infected MDDCs treated with low

107 HIV-1 env genes from macaque m584 (env(m584)) and from inocula

108 mined the functional properties of the HIV-1 env genes from six long-term survivors.

109 ed phenotypic and genetic variation in HIV-1 env genes from subjects in acute/early infection and sub

110 We cloned genetically divergent env genes from the plasma of two wild-infected sabaeus A

111 We have now cloned the env genes from the the parental and escape mutant isolat

112 Furthermore, evaluation of env genes from three chimpanzees infected with the same

113 In this study, we analyzed 37 full-length env genes from uncultured brain biopsy and blood samples

114 ing sequences derived from the viral gag and env genes fused to the myb coding region.

115 ency virus type 1 (HIV-1) tat, rev, vpu, and env genes generated pathogenic viruses (SHIV-89.6P) indu

116 Although the product of the env gene, gp160/gp120, is known to play a role in cell d

117 was shown that almost the entire retroviral env gene had been replaced by a sequence of cellular ori

118 Notably, proteins produced from envelope (env) genes have been shown to act as restriction factors

119 We found that HIV-1 lacking the env gene (HIVDeltaenv) still induced apoptosis in T-cell

120 s responsible have been mapped to within the env gene; however, it has remained unclear how env media

121 RV-T provirus in hominid genomes includes an env gene (hsaHTenv) that has been uniquely preserved.

122 t range, and sequence analysis of the CasE#1 env gene identifies segments related to PMVs and XMVs.

123 of the viruses, including the Vif, OrfA, and Env genes; (ii) the 5' end extending from the 5' long te

124 in the V1/V2 and C2/V3 regions of the HIV-1 env gene in 30 late-stage and 6 early-stage subjects.

125 evolution in the C2V3C3 region of the viral env gene in 8 HIV-2-infected individuals from Dakar, Sen

126 cation-competent MLV by replacing the native env gene in a full-length viral genome with that of anot

127 ly variable V3 or V4-V5 regions of the HIV-1 env gene in eight subjects, we found that all detectable

128 cellular receptor CD21, induces the HERV-K18 env gene in resting B lymphocytes.

129 inal repeat (LTR), a region that harbors the env gene in retroviral genomes.

130 as inserted after the last nucleotide of the env gene in the ecotropic FeLV-A Rickard (FRA) provirus.

131 Conversely, inactivation of the HIV-2 env gene in the original ROD10 clone resulted in a decre

132 Evolution of the env gene in transmitted R5-tropic human immunodeficiency

133 virus, we constructed chimeras encoding 81T env genes in a 61E background.

134 phenotype was transferable by expressing the env genes in an isogenic proviral DNA backbone, indicati

135 Moreover, clonally amplified virus env genes in milk produced functional virus Envs that we

136 nd new clonal amplification of evolved virus env genes in milk.

137 del for analyzing the functions of subtype-E env genes in viral transmission and pathogenesis and for

138 id resistance gene gpt in place of the viral env gene) in permissive cells, and we used them to gener

139 man cells in SCID-hu mice to a region of the env gene including the three most amino-terminal variabl

140 on of rev) open reading frames, the complete env gene (including the first exon of rev), and the 3' l

141 us vectors (Ad5 or Ad35) and HIV-1 envelope (Env) gene inserts (clade A or B) in a prime-boost regime

142 Transfer of the entire SHIV(DH12R-CL-7) env gene into the genetic background of nonpathogenic SH

143 One such env gene is CZA97.012 from a neutralization-resistant (t

144 ous studies of men have shown that the HIV-1 env gene is homogeneous early in infection, leading to t

145 The EnvD gene is carried on the P. noertemanii genome but co

146 opic SHIV carrying a primary pediatric HIV-C env gene isolated from a 2-month-old Zambian infant, who

147 se data led to the suggestion that the ALV-J env gene might have arisen by multiple recombination eve

148 HIV CCR5 antagonists select for env gene mutations that enable virus entry via drug-boun

149 This new env gene, not present in any other carnivoran, is a like

150 ts expressing molecularly cloned full-length env genes obtained from patient-derived HIV-1 isolates b

151 HIV-1 env genes obtained from seven mothers and their perinata

152 genetic events that led to truncation in the env gene occurred de novo in FeLV lymphomagenesis and th

153 These represent the only cases in which the env gene of a retrovirus has been traced back to its ori

154 ty and myeloid leukosis induction map to the env gene of ALV-J.

155 Interestingly, we find that the env gene of an anemic strain of Friend virus, Rauscher v

156 virus (EBV) transcriptionally activates the env gene of an endogenous retrovirus, HERV-K18, that pos

157 Analysis of the env gene of eight other HERV families indicated that rei

158 e gag/pol genes and the other expressing the env gene of FeLV-A/Glasgow-1.

159 The env gene of gammaretroviruses encodes a glycoprotein con

160 s in hypervariable regions (V1 to V3) of the env gene of HIV type 1 (HIV-1) 89.6.

161 A naturally arising point mutation in the env gene of HIV-1 activates the aberrant inclusion of th

162 s begun with a chimeric virus containing the env gene of HIV-1 HXBc2 and the gag and pol genes of sim

163 n vitro or in vivo, were introduced into the env gene of HIV-1(NL4-3) by site-directed mutagenesis an

164 d the complete variable region 3 (V3) of the env gene of HIV-1(YU2) or HIV-1(Ccon) to yield the chime

165 chimeric simian-human virus that encodes the env gene of HIV-IIIB.

166 x RNA stem-loop structure located within the env gene of HIV.

167 The env gene of human immunodeficiency virus type 1 (HIV-1)

168 cted by PCR a 660-bp sequence similar to the env gene of MMTV but not to the known endogenous viruses

169 an ITAM signaling domain encoded within the env gene of murine mammary tumor virus (MMTV).

170 tutions accumulated over time throughout the env gene of SHIVSF33A; some of them coincided with the a

171 the Rev responsive element (RRE) within the env gene of the HIV-1 RNA genome and is involved in tran

172 the Rev responsive element (RRE) within the env gene of the HIV-1 RNA genome, activating the switch

173 The env gene of the KU-1 virus was used to create a molecula

174 n expression levels of plasmid clones of the env gene of the MCF13 and noncytopathic NZB-9 MLV strain

175 gle transmembrane helix that is coded by the env gene of the polycythemic strain of the spleen focus

176 ied and sequenced the full-length RNA of the env gene of the type 1 and 2 HERV-K (HML-2) viruses coll

177 ng an SI phenotype and was restricted by the env gene of the virus.

178 Thus, the env gene of transmitted HIV-1 confers resistance to a la

179 ted by recombination of ecotropic MuLVs with env genes of a family of endogenous proviruses in mice,

180 presence of the same lethal mutation in the env genes of both FeLV and murine ERV provides a common

181 When the recombinant trimers based on the env genes of isolates 92UG037.8 and CZA97.012 were made

182 The sites of recombination between the env genes of M-MuLV and endogenous proviruses were confi

183 egulatory regions are located in the pol and env genes of MMTV.

184 2 cell line L5178Y and closely resembles the env genes of modified polytropic proviruses.

185 In the present study, the gag, pol, and env genes of SIV(K6W) were expressed in the NYVAC vector

186 Furthermore, genetic sequencing of the env genes of strains infecting the vaccinees did not rev

187 Our study aimed to analyze env genes of subtype-E viral strains, prevalent in Asia

188 e regions that are related to those found in env genes of the A to E subgroups, the majority of the s

189 uences of a simple retrovirus and gag-pol or env genes of the complex BLV.

190 (SHIV) that contained the tat, rev, vpu, and env genes of the HXBc2 strain of HIV-1 in a genetic back

191 e common laboratory strains contain proviral env genes of the xenotropic/polytropic subgroup of mouse

192 ke soluble, recombinant trimers based on the env genes of two isolates of human immunodeficiency viru

193 env genes of viruses transmitted to infants IP, but not

194 We used the HIV-1 env genes of well-defined, subject-matched M-tropic and

195 mbinant FeLVs in which the LTR and envelope (env) gene of FeLV-945, or the LTR only, was substituted

196 Here we show that the envelope (env) gene of JSRV has the unusual property that it can i

197 ncytin genes are fusogenic envelope protein (env) genes of retroviral origin that have been captured

198 Syncytins are fusogenic envelope (env) genes of retroviral origin that have been captured

199 ults suggest that the alteration maps in the env gene or in a gene whose product affects the maturati

200 These findings indicate that HIV-1 env genes, other viral genes, and even full-length viral

201 unodeficiency virus genome where the rev and env genes overlap, resulting in changes in the predicted

202 nv gene sequence was expressed in 66% of the env gene-positive human breast cancers.

203 ic MuLV with two distinct sets of polytropic env genes present in the genomes of inbred mouse strains

204 ) in addition to the canonical gag, pol, and env genes, presumably reflecting its limited tropism to

205 sition 427 in the C4 region of the HIV-2/vcp env gene product (VCP) gp120 as a key determinant for th

206 iple SRV antigens representing gag, pol, and env gene products by Western immunoblotting.

207 In CV-1 cells, palmitate was added to env gene products containing single mutations but was ab

208 ing target cells infected with vv-expressing env gene products from early isolates and HIV-1 IIIB wer

209 of target cells expressing laboratory strain env gene products might limit the detection of HIV-1 env

210 The env gene products, gp52 and gp36, have been positioned o

211 d with those stimulated with the full-length env gene products.

212 nant vaccinia virus (rVV) delivering the BLV env gene ranged from 30 to 65%.

213 late, higher fitness mapped to the subtype B env gene rather than the subtype C gag and pol genes.

214 leukemogenesis in mice is the appearance of env gene recombinants known as mink cell focus-inducing

215 itional 31 sexually transmitted Kenyan HIV-1 env genes, representing several recent infections with s

216 Thus, the HERV-W env gene represents the first HERV env gene demonstrated

217 ypical retroviruses, whereas others lack the env gene required for virions to enter cells and thus be

218 xpressing SIVmac239 or SIVmac251 gag/pol and env genes, respectively.

219 e subunit (TM) of the envelope glycoprotein (env) gene result in a different topology for some retrov

220 Our data showed that exchange of the env gene resulted in altered T-cell transformation tropi

221 CR analyses of plasma viral RNA indicated an env gene segment containing the V3 region from the inocu

222 of plasma HIV-1 RNA > 50 copies/ml and virus env gene sequence changes.

223 660-bp mouse mammary tumor virus (MMTV)-like env gene sequence in approximately 38% of human breast c

224 viral-experienced patients and that based on env gene sequence of the viruses in the patients.

225 This MMTV-like env gene sequence was expressed in 66% of the env gene-p

226 Phylogenetic analysis of C2-V3 env gene sequences confirmed that 94CY032.3 was closely

227 However, the three env gene sequences did not contain any polytropic sequen

228 exogenous ecotropic MuLVs are replaced with env gene sequences from an endogenous polytropic retrovi

229 In this study, env gene sequences from SHIV(SF162-p3)-infected rhesus m

230 cular, is limited since only about 400 bp of env gene sequences have been determined for just two epi

231 In these recombinants the env gene sequences of exogenous ecotropic MuLVs are repl

232 proviruses that are potential donors of the env gene sequences of polytropic MuLVs; however, the pre

233 gical studies, the HIV-1 tat and part of the env gene sequences of the longitudinal isolates at four

234 HIV gag, pol, and env gene sequences were consistent with a clonal relatio

235 vestigate their validity for HIV, samples of env gene sequences were tested for departure from neutra

236 the pol gene and various amounts of gag and env gene sequences.

237 Env gene sequencing from NAb-resistant viruses was used

238 sociated with the central region of the Fr98 env gene showed no upregulation of cytokines or chemokin

239 thern China, where partial sequencing of the env gene showed subtype C and circulating recombinant fo

240 affinity for IgG1b12, and sequencing of its env gene showed that amino acid substitutions had occurr

241 iruses and their acquisition of an envelope (env) gene, similar independent events may have occurred

242 ogenous feline leukemia virus (FeLV)-related env gene species from lymphosarcomas induced by intrader

243 verse an imbalance in splicing caused by the env gene substitution.

244 Phylogenetic analyses of gag and env genes suggest that chains of transmission frequently

245 rmined for the gag and pol genes but not the env gene, suggesting that a recombination event may have

246 rs: the LTR and a second promoter within the env gene termed the internal promoter (IP).

247 -fold lower genetic diversity (<0.01 s/n) in env gene than other EC.

248 nt, SIV/17E-Cl, contained the portion of the env gene that encodes the surface glycoprotein and a sho

249 ha secretion mapped to a region of the HIV-1 env gene that includes the third hypervariable domain.

250 enotypic and phenotypic changes in the viral env gene that occur in the viral populations of DKO-hu-H

251 Escape virus contained mutations in the env gene that were unexpectedly sparse, did not map gene

252 tribution of V3 sequence variation, chimeric Env genes that contained consensus V3 sequences from sev

253 promoter, located towards the 3' end of the env gene, that directs expression of the viral auxiliary

254 e virulence of these viruses maps within the env gene, the mechanism of fusion enhancement is not ful

255 RV lineages, we show that when ERVs lose the env gene their proliferation within that genome is boost

256 the methods may have wider utility for other env genes, thereby further guiding immunogen design.

257 /V2) and variable region 3 (V3) of the HIV-1 env gene to analyze the effect of a genetic bottleneck c

258 in order to deliver the neurovirulent CasBrE env gene to endogenous CNS cells.

259 ion and sequence analysis of the full-length env gene to identify CSF-compartmentalized variants and

260 ate the evolutionary potential of retroviral env genes to alter receptor usage in response to appropr

261 Here, we used the CZA97.012 and 92UG037.8 env genes to compare some of these designs and determine

262 endpoint dilution PCR followed by cloning of env genes to create pseudotyped virus to explore the lin

263 region of the surface envelope glycoprotein (env) gene to investigate the replication and cellular tr

264 cine candidate vector expressing HIV gag and env genes, vCP205, was modified by the introduction of a

265 roteins (gp140UNC-Fd-His), based on the same env genes, very rarely form native-like trimers, a findi

266 A full-length ancestral subtype B HIV-1 env gene was constructed and shown to produce a glycopro

267 sequencing of the V1/V3 region of the HIV-1 env gene was done for paired blood and semen samples fro

268 specific sequence in the C2-V3 region of the env gene was found in 46 HIV-1-infected women.

269 Single genome amplification for the HIV env gene was performed on genomic DNA extracts from diff

270 Deep sequencing of the HIV DNA partial env gene was performed, and the dynamics of viral divers

271 ated with the N-terminal portion of the Fr98 env gene was preceded by upregulation of cytokines and c

272 the United States and a 620 nt region of the env gene was sequenced for each sample.

273 A series of chimeras from NS and NR env genes was constructed, and each was presented on pse

274 immunodeficiency virus sm (SIVsm) envelope (env) gene was analyzed in relation to route of virus cha

275 To characterize this env gene, we examined resistance in crosses between Rmcf

276 e phosphoribosyl transferase in place of the env gene, we generated cell lines resistant to mycopheno

277 contains multiple copies of xenotropic MuLV env genes, we suggest that these resistance genes contro

278 eterogeneity in protection, fragments of the env gene were amplified from peripheral blood mononuclea

279 on, the V1/V2 and V3 variable regions of the env gene were examined by using a heteroduplex tracking

280 and secondary protease cleavage sites of the env gene were identified that resulted in the stable sec

281 fact that the endogenous subgroup J-related env genes were associated with long terminal repeats (LT

282 kine receptor utilization, full-length HIV-1 env genes were cloned from latently infected cells and a

283 Full-length functional env genes were cloned longitudinally from these subjects

284 env genes, expression plasmids for the three env genes were constructed and used to generate retrovir

285 hritis; for a subset of samples, full-length env genes were generated for sequence analysis and to te

286 Full-length gag and env genes were recovered directly from peripheral blood

287 A series of 22 chimeric envelope (env) genes were generated between the ecotropic Moloney

288 In this study, HIV envelope (env) genes were sequenced from virus variants amplified

289 g-pol coding region and/or the viral nef and env genes, were designed.

290 We constructed a series of mutant MuLV env genes which express Env proteins with serial truncat

291 me indicates that neurovirulence maps to the env gene, which encodes the surface glycoprotein respons

292 Our results suggest that the env gene, which is linked to a myriad of viral character

293 We show that all genes, including the env gene, which is necessary only for movement between c

294 ngle nucleotide polymorphism in the ERV-DC14 env gene, which results in a replication-defective produ

295 ovirus genome and entirely overlapped by the env gene with reading frame -2.

296 and screened for the presence of retroviral env genes with a full-length ORF.

297 In silico search for env genes with full coding capacity identified several c

298 An in silico search for env genes with full coding capacity within a Tenrecidae

299 rent factor-independent cell clones revealed env genes with open reading frames encoding 644, 449, an

300 can be recovered in vivo by mutations in the env gene, without an associated pathogenic phenotype, an