ライフサイエンスコーパス: enzymology

1 represent a new paradigm in natural product enzymology.

2 chemical bonds is an evolving perspective in enzymology.

3 molecular evolution, molecular biology, and enzymology.

4 to elucidate the N,C-bipyrrole homocoupling enzymology.

5 s an exciting and challenging area of modern enzymology.

6 nsic reaction-cycle time of about 0.4 s from enzymology.

7 M is powerful for structural and mechanistic enzymology.

8 nts a fundamental challenge in assembly line enzymology.

9 cusing on their biochemistry, structure, and enzymology.

10 prominent position in the field of helicase enzymology.

11 identified that parallel those encounterd in enzymology.

12 tG) presents a new challenge in heme protein enzymology.

13 irst proposal of microenvironment effects in enzymology.

14 wer has been one of the challenges of modern enzymology.

15 tion assay that requires no amplification or enzymology.

16 , metabolite screening, complementation, and enzymology.

17 uctures, have led to real insight into their enzymology.

18 polyketide synthase and fatty acid synthase enzymology.

19 interaction mechanism in NRPS assembly line enzymology.

20 hydrogen-transfer reactions in chemistry and enzymology.

21 is one of the major challenges in nonaqueous enzymology.

22 is unprecedented in ADP-ribosyl transferase enzymology.

23 abolism and have been paradigms of classical enzymology.

24 ive-site with only modest affect on myosin's enzymology.

25 ature and opens new avenues in chemistry and enzymology.

26 general use in cell biology, metabolism, and enzymology.

27 in terms of both structural diversities and enzymology.

28 has been a long-standing goal of structural enzymology.

29 to parameters influencing single nanodroplet enzymology.

30 hat is rare in conventional two-electron PLP enzymology.

31 nting the way to future frontiers in radical enzymology.

32 are not known in either organic chemistry or enzymology.

33 ar or greater sensitivity to the traditional enzymology.

34 ues to drive advances in protein science and enzymology.

35 enabling new approaches to study Integrator enzymology.

36 tions has been a fundamental focus in modern enzymology.

37 matics, computational biology, and molecular enzymology.

38 tigate fundamental and practical problems in enzymology.

39 ading domain in the context of assembly-line enzymology.

40 ave been made toward understanding their (1) enzymology, (2) role as critical components of biologica

41 To investigate DNA replication enzymology across the nuclear genome of budding yeast, d

42 The results of steady-state enzymology analysis were consistent with allostery and s

43 To improve our understanding of the enzymology and activation mechanism of Syk, we character

44 ht into analogous behavior in biophysics and enzymology and aims to inform the design of efficient se

45 regulated modifications that alter PKCdelta enzymology and allow for stimulus-specific control of ca

46 More generally, comparative enzymology and analysis of catalytic promiscuity can pro

47 As synthetic biologists continue to improve enzymology and analytical chemistry to support AEGIS-LIV

48 es such regions based on the presence of new enzymology and based on patterns of gene cluster and pre

49 has opened a wealth of new opportunities in enzymology and biocatalysis research.

50 While the enzymology and biological consequences have been extensi

51 entified and characterized, both in terms of enzymology and biological function.

52 e used in synthetic biology, single-molecule enzymology and biophysical analysis, as well as portable

53 Through comprehensive structural, enzymology and cellular characterization of DNMT3A and D

54 Using a combination of genomics, enzymology and crystallography, we show that the mucin-d

55 al condensates focusing on basic concepts in enzymology and discussing some context-dependent roles o

56 w provides a comprehensive overview of their enzymology and distinct roles in glucose homeostasis.

57 enzymes is a fundamental challenge for both enzymology and drug design.

58 ed for high-throughput screening methods for enzymology and drug discovery.

59 ions and drive new advances in the fields of enzymology and dynamic processes.

60 Based on a combination of enzymology and gene-analysis, a new degradative pathway

61 Although the enzymology and genetics of PHB metabolism in S. meliloti

62 Here we describe the crystal structure, enzymology and in vitro activity on human substrates of

63 adical chemistry common to both coenzyme B12 enzymology and its known photochemistry.

64 A description of the enzymology and mechanism of terpenoid cyclization is fol

65 entury witnessed many advances in scientific enzymology and microbiology that laid the foundations fo

66 Our results illustrate how classical enzymology and modern protein design can each inform the

67 Progress in enzymology and molecular biology of SA biosynthesis and

68 Finally, enzymology and molecular dynamics simulations indicate t

69 The enzymology and molecular genetics of biosynthesis of pht

70 nificant changes in our understanding of the enzymology and molecular mechanisms of these DMSP cyclin

71 een directly demonstrated by single molecule enzymology and NMR studies recently.

72 sive functional experiments advance membrane enzymology and propel the development of treatments for

73 ntly discovered peptides, review advances in enzymology and protein engineering, and discuss the regu

74 important information for understanding the enzymology and regulation of L- and S-SMase and for expl

75 rve as an excellent model for studies of the enzymology and regulation of this protein.

76 n an enzyme very similar to wild type in its enzymology and secondary structure, indicating that this

77 sults expand our understanding of telomerase enzymology and should encourage the development of paras

78 tudies of lambda biology, lambda exonuclease enzymology and the availability of the X-ray crystallogr

79 sing knowledge about nuclear DNA replication enzymology and the rate and specificity with which misma

80 broadening our understanding of biosynthetic enzymology and the regulation of BGCs.

81 reflections on these studies that began with enzymology and the use of cultured mammalian cells, and

82 ion of the newly generated FMS constructs by enzymology and thermoshift assays demonstrated similar a

83 able fundamental breakthroughs in chemistry, enzymology and translational applications of enediyne na

84 s advanced our knowledge of isoform-specific enzymology and will facilitate the discovery of novel PI

85 Distinct functions shape distinct enzymologies, and this is illustrated by comparing kines

86 familiar arithmetic concepts from classical enzymology, and also about connections between modeling

87 ompelling problem in fundamental mechanistic enzymology, and as part of our mechanistic studies of th

88 We draw inspiration from this structural enzymology, and design a two-component metal-sulfur-carb

89 heric sensing, chemical reaction monitoring, enzymology, and live-cell imaging.

90 e the advantages of heterogeneous catalysis, enzymology, and molecular catalysis represents the next

91 -molecule catalysis and synthetic chemistry, enzymology, and molecular evolution to discover or creat

92 he challenges in protein design, mechanistic enzymology, and molecular evolution.

93 rotein-protein interaction assays, G protein enzymology, and morphological analysis, we demonstrate h

94 combination of virology, molecular biology, enzymology, and protein structural and modeling studies

95 dvance studies in terpenoid biosynthesis and enzymology, and provide model systems to probe the ecolo

96 a combination of computational predictions, enzymology, and structural biology.

97 Using a large-scale "genomic enzymology" approach, we (i) assigned novel ATP-dependen

98 which limits the utility of well-established enzymology approaches developed under the assumption of

99 Biochemical, super-resolution imaging, and enzymology approaches establish that this Gravin scaffol

100 ation of cell biology, chemical biology, and enzymology approaches to analyze this processing event.

101 that exemplify how key tools in mechanistic enzymology are valuable time and again.

102 form has high potential impact in label-free enzymology as well as in drug discovery.

103 lar dynamic (MD) simulations, phylogeny, and enzymology assays to provide molecular insights into Mg(

104 ubstrate and product peptides, combined with enzymology assays, show that this enzyme targets the N-t

105 etazoa raises the prospect of an alternative enzymology based on covalently activated 3' ends.

106 ta-reductase is a unique reaction in steroid enzymology because hydride transfer from NADPH to the be

107 apidly recycled to inositol, and uncover the enzymology behind an alternative "soluble" route to synt

108 tors and their far-reaching implications for enzymology, biocatalysis, and biotechnology.

109 t have challenged fundamental assumptions in enzymology, biochemistry and cell metabolism regarding t

110 terdisciplinary fields such as biochemistry, enzymology, biofuels, bioengineering and drug discovery.

111 ylation is an ancient process with conserved enzymology but diverse biological functions that include

112 hanism of sulfoxide synthases by comparative enzymology, but also raises the question as to why so ma

113 pping is an important mechanism in molecular enzymology, but its study is limited by the lack of an a

114 established a new mode of radical pyridoxal enzymology by leveraging open-shell enamine catalysis, o

115 ry-holds significant promise for mechanistic enzymology: by providing atomic-resolution characterizat

116 Biologists working in topological enzymology can use this program to compute and visualize

117 ly pose fascinating questions of metabolism, enzymology, cell biology, and regulation; the answers ha

118 unprecedented view of lactate dehydrogenase enzymology, confirming the timescale of substrate-induce

119 e histology, contractile protein content and enzymology contribute to exercise intolerance in CHF.

120 ructure-based drug design (SBDD), synthesis, enzymology data, and X-ray crystallography results are d

121 lustrate the application of ABPs to protease enzymology, discovery and development of protease-target

122 that pseudomonads utilize sulfite reduction enzymology distinct from that of E. coli and possibly si

123 ding experiments helped illuminate the novel enzymology driving ecteinamine assembly as well the role

124 a powerful tool for protein engineering and enzymology, drug discovery, and proteomics.

125 r, KIEs have not come into widespread use in enzymology, due in large part to the requirement for pro

126 the practical development and application of enzymology, e.g., in the interpretation and prediction o

127 he need for in-depth research in solid-state enzymology, especially in multi-step enzymatic cascades,

128 and postdoctoral training in metabolism and enzymology eventually led me to study the short- and lon

129 Sequence alignments, enzymology experiments and inhibitor studies on p300/CBP

130 n was overexpressed in Escherichia coli, and enzymology experiments demonstrated its unsaturated beta

131 Enzymology experiments show that the MDDEF performs a se

132 Bulk enzymology experiments verify that the enzyme is noncomp

133 The predictions were tested by enzymology experiments, and Arad3529 deaminated many pte

134 with free energy simulations, virology, and enzymology, explain the mechanisms of DTG resistance inv

135 netics of this molecular motor to see if its enzymology explains this remarkable degree of processivi

136 attention through discoveries concerning its enzymology, extent, and function.

137 sights into how these two motors adapt their enzymologies for their distinct functions.

138 nd untapped potential of targeting bacterial enzymology for improvements in human health.

139 cleic acid pre-amplification with CRISPR-Cas enzymology for specific recognition of desired DNA or RN

140 ich may have applications in many aspects of enzymology, from the design of novel enzymes to the pred

141 Entry into lignan enzymology has been gained by the approximately 3000-fol

142 Membrane protein enzymology has been hampered by the inherent insolubilit

143 Single-molecule enzymology has come a long way since the early demonstra

144 Radical S-adenosylmethionine (RS) enzymology has emerged as a major biochemical strategy f

145 However, PBP-transpeptidation enzymology has evaded detailed analysis, because of the

146 In contrast, bipyrrole-coupling enzymology has not been observed.

147 The study of membrane protein structure and enzymology has traditionally been hampered by the inhere

148 In vitro studies of lasso peptide enzymology have been hampered by difficulties in obtaini

149 A cherished tenet of nucleic acid enzymology holds that synthesis of polynucleotide 3'-5'

150 me function and demonstrates single-particle enzymology in a system of more than two components.

151 er, our work revealed the chemical logic and enzymology in extending the biosynthetic pathway of a we

152 erest in biological chemistry proceeded from enzymology in vitro to the study of physiological chemis

153 e-binding C2 domain that controls PKCdelta's enzymology indirectly by decreasing phosphorylation in t

154 In classical enzymology, intermediates and transition states in a cat

155 ribed to the acetamide group and the unusual enzymology involved, we enzymatically interrogated diver

156 even less has been established regarding the enzymology involved.

157 A significant contemporary question in enzymology involves the role of protein dynamics and hyd

158 uncover a new catalytic motif in radical SAM enzymology involving the use of two 5'-deoxyadenosyl rad

159 Crystallography and enzymology involving WT and Ala190 uPA were used to calc

160 Computational enzymology is a rapidly maturing field that is increasin

161 defense, an understanding of the underlying enzymology is lacking.

162 evidence for their catalytic significance in enzymology is lacking.

163 The main focus of enzymology is on the enzyme rates, substrate structures,

164 s particularly applicable to single-molecule enzymology is presented.

165 A substantial challenge for genomic enzymology is the reliable annotation for proteins of un

166 A central goal of enzymology is to understand the physicochemical mechanis

167 e of the most intriguing questions in modern enzymology is whether enzyme dynamics evolved to enhance

168 utionizing structural biology, its impact on enzymology is yet to be fully demonstrated.

169 proaches uncovered unique aspects of Mtb Clp enzymology, its essentiality complicates in vivo studies

170 ty has grown from many directions: genetics, enzymology, kinetics, physical biochemistry, and thermod

171 This is achieved by adopting an enzymology-like framework, where transfer functions are

172 approach of substrate heat pre-treatment and enzymology may be used to influence proteolysis with att

173 nation of stereoselective organic synthesis, enzymology, microbiology, and X-ray crystallography was

174 position of this internal clock by combining enzymology, molecular biology, genetics, and modeling ap

175 ding paradigm in modular polyketide synthase enzymology, namely the definition of a module, has been

176 plications as diverse as structural biology, enzymology, nanotechnology and systems biology.

177 dings from this study are expected to impact enzymology, natural product biosynthesis, and future eff

178 The enzymology of 135 assembly lines containing primarily ci

179 biosynthesis, the recycling of NADH, and the enzymology of 2-hydroxyacid dehydrogenases are discussed

180 We now report the synthesis, structure, and enzymology of a set of 20 smSNAREs (small molecule SNAr-

181 amplification of RNA targets is based on the enzymology of a single thermostable DNA polymerase and t

182 ss in the understanding of the chemistry and enzymology of abasic DNA largely relies upon the study o

183 We show here that the enzymology of an AARS urzyme*TPsiC-minihelix cognate pai

184 dation for quantitatively characterizing the enzymology of any number of clinically relevant LPLAT pr

185 The enzymology of bacterial lignin breakdown is currently no

186 However, little is known about the enzymology of BER of altered in-tandem CpG dinucleotides

187 The enzymology of CD39L2 shows characteristic features of a

188 sary forerunner to defining the genetics and enzymology of cell wall polymer-peptidoglycan ligation i

189 hich integrated cellular coordination to the enzymology of clot formation and was conceptualized duri

190 In efforts to understand the complex enzymology of CYP17, structure/function relationships ha

191 tida KT2440 to elaborate on the genomics and enzymology of d-amino acid metabolism.

192 the role of a flavin reductase, DszD, in the enzymology of desulfurization, and to the use of new too

193 cellular dysfunction and tissue damage, the enzymology of FAEE metabolism remains poorly understood.

194 To better understand the enzymology of hemoglobin degradation, it is necessary to

195 solve a long-standing riddle about the basic enzymology of IDE with important implications for the et

196 ith other nuclear hormone receptors, yet the enzymology of its physiological generation remains uncle

197 While the enzymology of lambda-terminase has been well described,

198 ubstituents is necessary for elucidating the enzymology of lipid A biosynthesis and for characterizin

199 However, little is known about the enzymology of mitochondrial O-GlcNAcylation.

200 d level of genetic complexity underlying the enzymology of N-terminal myristoylation and suggest that

201 le tools to study the structural biology and enzymology of NAD+ binding and consuming enzymes, such a

202 To date, the polarity and enzymology of NMD in mammalian cells is unknown.

203 To study the enzymology of OGT, independent of potential regulatory p

204 However, the biological functions and enzymology of p68 RNA helicase are not well characterize

205 The cellular functions, regulation and enzymology of phosphatidylinositol (PtdIns) 5-P, the new

206 f PKS domains that expands the chemistry and enzymology of PKSs and might be exploited to incorporate

207 The emphasis in this review is on the enzymology of prenyl protein processing and the function

208 loration of intein structure and the unusual enzymology of protein splicing.

209 In this study, the enzymology of replication past site-specific 1,N(6)-gamm

210 The enzymology of RNase YI*, a recently discovered endoribon

211 n major advances in our understanding of the enzymology of sirtuins, their regulation, and their abil

212 lable and their mechanistic details, (e) the enzymology of some of the key reactions required for rep

213 neous fluorescence-based method to study the enzymology of specialized DNA polymerases in real time.

214 chain dehydrogenases/reductases and into the enzymology of steroid and retinoid metabolism.

215 Understanding the enzymology of such enzymes should provide the informatio

216 ral approaches are now shedding light on the enzymology of the APC.

217 an important gap in our understanding of the enzymology of the assembly of coenzyme B(12).

218 The genetics and enzymology of the biosynthesis of wall teichoic acid hav

219 past few years, much knowledge of the basic enzymology of the Cdc25 phosphatases that may aid in the

220 Although the enzymology of the DD-carboxypeptidases has been studied

221 versify the linear peptide both to probe the enzymology of the macrocyclizing enzyme, TycC thioestera

222 spired many total synthesis efforts, but the enzymology of the okaramine biosynthetic pathway remains

223 ts, but much remains to be learned about the enzymology of their formation.

224 ned and opens the way to direct study of the enzymology of this complex biosynthetic pathway.

225 In addition, we examine the basic enzymology of this enzyme through molecular modeling stu

226 r location of Ii degradation, as well as the enzymology of this event, are important in determining t

227 To study the enzymology of this interesting process we have expressed

228 lmost nothing is known about the function or enzymology of this modification.

229 Understanding the enzymology of this pathway will be key to success for th

230 Here we study the enzymology of three human RNA methyltransferases that me

231 Recent advances in the enzymology of transcription and chromatin regulation hav

232 In the present study we have examined the enzymology of ubiquitination of endogenous InsP3Rs in mu

233 l utility in our global understanding of the enzymology of virus assembly.

234 The enzymology of VLDL triglyceride synthesis is still poorl

235 tensively studied, little is known about the enzymology of volatile aldoxime formation.

236 nds for NTE inhibition and investigating the enzymology of wild-type and mutant forms of NTE.

237 phy, molecular dynamics simulations, and DNA enzymology on the Saccharomyces cerevisiae Rev1 protein.

238 Detailed enzymology on this enzyme has been limited, due to diffi

239 ck, novel approaches based on new chemistry, enzymology or next generation sequencing have emerged.

240 etic reaction, which has little precedent in enzymology or organic synthesis, expands the catalytic c

241 Little is known about the enzymology or regulation of Mg-chelatase, as it has been

242 es in fields as varied as organic chemistry, enzymology, or protein folding.

243 recent advancements in characterizing their enzymology, our knowledge about the dynamic processes of

244 We present enzymology, pathway organization, and evolutionary analy

245 Single molecule enzymology provides an opportunity to examine details of

246 Single-molecule enzymology provides an unprecedented level of detail abo

247 nd energetic analysis, fluorescence studies, enzymology, pulse proteolysis, X-ray crystallography, an

248 omerase, fundamental questions regarding its enzymology remain unanswered.

249 Decades of enzymology research has established general themes regar

250 major issue for both structural genomics and enzymology research.

251 of unusual bioactive compound structure and enzymology, respectively.

252 Enzymology revealed that the Psd2(C2Delta)p and Psd2(GRD

253 Here, we use single-molecule enzymology (SME) to monitor the catalytic recovery of ur

254 Here, by combining enzymology, structural biology, and activity-based metab

255 t understanding of the microbial physiology, enzymology, structural biology, and folding of the proka

256 Recent advances in enzymology, structural biology, and protein chemistry ha

257 SIRT6 amino acid substitutions identified in enzymology studies and activity-selective compounds coul

258 utagenesis of the triad residues followed by enzymology studies of mutant and wild-type hBATs.

259 onstrate the utility of AMPL for mechanistic enzymology studies, an (18)O-labeled substrate was gener

260 o understand the changes in biochemistry and enzymology that accompanied adaptation to O2, we integra

261 logical opportunities afforded by nonaqueous enzymology, the issue of often drastically diminished en

262 ocomposites straddle materials chemistry and enzymology, this review provides an assessment of the ch

263 structure and regulation, protein structure, enzymology, tissue distribution and pathophysiological r

264 , and computational tools brings mechanistic enzymology to a new era, one poised for novel fundamenta

265 as been accompanied by a shift of focus from enzymology to a search for substrates as well as the fir

266 e the last frontier in protein chemistry and enzymology to be conquered.

267 m natural products discovery and mechanistic enzymology to elucidate the structure and biosynthesis o

268 lecular dynamics simulations, synthesis, and enzymology to explore potential pharmacophoric features

269 In this study, we use cryo-EM and enzymology to explore SIRT6 preference and adaptability

270 avior requires specific adaptations of motor enzymology to meet these unique physiologic demands.

271 nt form due to the application of industrial enzymology to solve process related problems.

272 Our work suggests use of the nanopore as an enzymology tool and provides a means to identify single

273 clude multiple hosts and expression systems, enzymology tools for in vitro study, and ways to enginee

274 Despite great interest in CYP enzymology, two in vitro aspects of CYP3A4 catalysis are

275 stingly, aminomalonyl-ACP formation involves enzymology typically found in nonribosomal peptide synth

276 ies have contributed to our knowledge of the enzymology underlying acetylation, including efforts to

277 ps and suggests some interesting features of enzymology using a surface-bound substrate.

278 Based on RNase H enzymology, we enhanced single nucleotide discrimination

279 (EFI) provides a web resource with "genomic enzymology" web tools to leverage the protein (UniProt)

280 r to the kinase by x-ray crystallography and enzymology while demonstrating potency, selectivity, and

281 Using in vitro enzymology with soluble kinase constructs, we establishe

282 Here, in crystallo enzymology with the catalytically active bacterial cellu

283 a mechanism distinct from ubiquitylation in enzymology yet analogous to ubiquitylation in targeting