ライフサイエンスコーパス: ethidium bromide

1 after cell staining with acridine orange and ethidium bromide).

2 gand, and to the classic intercalating agent ethidium bromide.

3 ethidine in the extracellular environment to ethidium bromide.

4 mycin, bisphenol A, chlorinated phenols, and ethidium bromide.

5 ed by gel electrophoresis in the presence of ethidium bromide.

6 et by fluorescence after brief staining with ethidium bromide.

7 and measured mtDNA after 3-d treatment with ethidium bromide.

8 demyelinated by the intraspinal injection of ethidium bromide.

9 by x-ray irradiation and focal injections of ethidium bromide.

10 d after electrophoresis in 1.5% agarose with ethidium bromide.

11 oethidium, a membrane-permeant derivative of ethidium bromide.

12 lbenzene and isoeugenol were associated with ethidium bromide.

13 by agarose concentration or the presence of ethidium bromide.

14 x-irradiation and intraspinal injections of ethidium bromide.

15 sible DRB4 band in agarose gels stained with ethidium bromide.

16 equires UV radiation and the fluorescent dye ethidium bromide.

17 ly studied MDR substrates, Hoechst 33342 and ethidium bromide.

18 induced upon incubation with erythromycin or ethidium bromide.

19 ump (EP) in association with norfloxacin and ethidium bromide.

20 er rapidly, as revealed after treatment with ethidium bromide.

21 ntibiotics based on the fluorescent molecule ethidium bromide.

22 served fluorescently after labeling DNA with ethidium bromide.

23 thus eliminating the need for staining with ethidium bromide.

24 ophoresis on a 0.8% agarose gel stained with ethidium bromide.

25 agarose gel electrophoresis and stained with ethidium bromide.

26 d in increased accumulation of intracellular ethidium bromide.

27 t)DNA by passaging in a low concentration of ethidium bromide.

28 on uridine after eight passages in 50 ng/mL ethidium bromide.

29 nd intercalating ligands: DAPI, Hoechst, and ethidium bromide.

30 Of the 6 compounds that did not displace ethidium bromide, 2 also inhibited B-ZIP binding to DNA

31 The cation of the salt ethidium bromide (3,8-diamino-5-ethyl-6-phenylphenanthri

32 ansfected with hCx31.9-EGFP took up DAPI and ethidium bromide 5-10 times faster than wild-type cardio

33 More importantly, cytosine arabinoside, ethidium bromide, 5-azacytidine and aspirin all signific

34 dino-2-phenylindone (an AT-specific binder), ethidium bromide (a nonspecific binder), and chromomycin

35 A reduction in the MIC of ethidium bromide (a substrate for several efflux pumps)

36 oparticles capped with DNA intercalated with ethidium bromide, a fluorescent molecule.

37 optosis was determined by DNA fragmentation, ethidium bromide-acridine orange nuclear stain and TdT-m

38 PI viability was examined with ethidium bromide-acridine orange, and apoptosis was exam

39 DNA fragmentation and ethidium bromide/acridine orange (EB/AO) nuclear stainin

40 ted for apoptosis either by staining with an ethidium bromide/acridine orange mixture (AO/EB) or with

41 viable cell recovery (VCR) with trypan blue, ethidium bromide/acridine orange staining, and terminal

42 aternary ammonium on an aromatic ring (e.g., ethidium bromide, acriflavine hydrochloride, 2-N-methyle

43 PMNL were stained with acridine orange and ethidium bromide after 0, 3, 6, and 18 h in culture, and

44 ed products were visualized by staining with ethidium bromide after electrophoresis in 1.5% agarose.

45 ; staining at the single-molecule level with ethidium bromide after exhaustive deproteinization of ly

46 Ethidium bromide also inhibited NaeI-L43K, implying that

47 Unwinding the supercoiled DNA with ethidium bromide also made DNA resistant to AN/L3.

48 Ethidium bromide and 2',7'-dichlorofluorescein, fluoresc

49 The intercalating agents ethidium bromide and 9-aminoacridine enhanced oxopropeny

50 The system uses dye-based detection with ethidium bromide and a single DNA polymerase-based PCR o

51 nalyzed from single islet cells stained with ethidium bromide and acridine orange, apoptosis using a

52 ld decrease in sensitivity to quinolones and ethidium bromide and an increase in the level of norA tr

53 MDR-dependent efflux of ethidium bromide and berberine from S. aureus cells was

54 ycin, rifampin, novobiocin, and dyes such as ethidium bromide and crystal violet and increased accumu

55 For comparison, the unwinding angles of ethidium bromide and DA have been independently calculat

56 NA intercalating agents propidium iodide and ethidium bromide and enhanced by the presence of synthet

57 ical and B-DNA, displacement of intercalated ethidium bromide and facilitate cooperative binding of H

58 the displacement of DNA duplex intercalated ethidium bromide and gel electrophoresis.

59 ies of complexes with DNA in the presence of ethidium bromide and Hoechst 33258 revealed that minor g

60 binds to genomic DNA to a similar extent as ethidium bromide and Hoechst 33258.

61 Fluorescent quenching of ethidium bromide and of rhodamine covalently attached to

62 thod is at least 50-fold more sensitive than ethidium bromide and permits detection of </=0.25 ng dou

63 Decreased accumulation of ethidium bromide and rhodamine 6G in the hns mutant comp

64 -ethylmaleimide but is strongly inhibited by ethidium bromide and vanadyl ribonucleoside complexes.

65 ramphenicol), transcription and replication (ethidium bromide), and function (rotenone, rhodamine 6G)

66 ase in resistance to hydrophilic quinolones, ethidium bromide, and cetrimide and also to sparfloxacin

67 rmeable to Lucifer yellow, Alexa Fluor(350), ethidium bromide, and DAPI, which have valences of -2, -

68 cobacterium smegmatis is more susceptible to ethidium bromide, and drug resistance is restored by the

69 substrates such as N-phenyl-1-napthylamine, ethidium bromide, and Hoechst dye in K. pneumoniae overe

70 nts are more sensitive than the wild type to ethidium bromide, and K. lactis sir4 mutants are more re

71 rculosis iniA in BCG conferred resistance to ethidium bromide, and the deletion of iniA in M. tubercu

72 resensitization of Hsmr-expressing cells to ethidium bromide; and was non-hemolytic to human red blo

73 o oligonucleotides with model intercalators (ethidium bromide andactinomycin D) and minor groove bind

74 rphology, DNA fragmentation, acridine orange/ethidium bromide (AO/EB) staining, cell cycle arrest, mi

75 a competitive binding strategy that utilizes ethidium bromide as a nonspecific binder to competitivel

76 imal sample preparation is possible by using ethidium bromide as the intercalating dye.

77 the use of simple DNA intercalators, such as ethidium bromide, as tools to facilitate the error-free

78 lectrophoretic mobilities in the presence of ethidium bromide before and after relaxation by calf thy

79 th DNA in electrophoretic mobility shift and ethidium bromide binding assays.

80 interstrand cross-links were measured by the ethidium bromide binding fluorescence assay and quantita

81 More than one ethidium bromide binding site is found in the acceptor s

82 Metal ions also weaken ethidium bromide binding to IRE-RNA with no effect on IR

83 ns in the DNA helix as detected by decreased ethidium bromide binding.

84 Low (micromolar) concentrations of ethidium bromide block RNase III[DeltadsRBD] cleavage of

85 Unlike ethidium bromide, both eilatin and the eilatin-containin

86 with a significantly longer lifetime (e.g., ethidium bromide bound to DNA), multipulse pumping and t

87 from sodium dodecyl sulfate, novobiocin, and ethidium bromide but failed with other known substrates

88 der-surpassing the sensitivity achieved with ethidium bromide by 200-fold.

89 binding (measured as accessibility of DNA to ethidium bromide by electrophoresis and by fluorescence

90 Moreover, ethidium bromide can be readily removed using isoamyl al

91 We show that ethidium bromide can influence DNA self-assembly, decrea

92 hat DXR and other DNA intercalators, such as ethidium bromide, can rapidly intercalate into mtDNA wit

93 In addition to hypersensitivity to ethidium bromide, cells that lack the lprG-Rv1410c opero

94 e insertion efficiency, and to resistance to ethidium bromide collectively demonstrate that EmrE mono

95 o footprinting of the latter showed ATP- and ethidium bromide-dependent modifications that could be c

96 Ethidium bromide depleted both mutant and normal cells o

97 nding affinity of polyamines to DNA using an ethidium bromide displacement assay showed that homologu

98 o screened for DNA binding efficacy using an ethidium bromide displacement assay.

99 e, circular dichroism, linear dichroism, and ethidium bromide displacement assays, which demonstrated

100 formation (by 2-aminopurine fluorescence and ethidium bromide displacement); (ii) metal ions increase

101 contrast, other DNA-binding agents, such as ethidium bromide, distamycin, and doxorubicin, inhibit t

102 ransfer takes place between DNA-intercalated ethidium bromide (DNA-EB) and the electrostatically boun

103 Ethidium bromide does not promote the formation of any o

104 showed potent synergistic activity with the ethidium bromide dye in a strain overexpressing the MepA

105 ble to remyelinate demyelinated axons inside ethidium bromide (EB) demyelination lesion in adult spin

106 ircular dichroism (CD) spectroscopy, and the ethidium bromide (EB) displacement assay.

107 rbance we measured changes in geometric mean ethidium bromide (EB) fluorescence intensities in subpop

108 rescence resonance energy transfer (FRET) to ethidium bromide (EB) intercalated within double-strande

109 Ethidium bromide (EB) is known to inhibit cleavage of ba

110 ng; energy is transferred from the CCP to an ethidium bromide (EB) molecule intercalated into the dsD

111 a cells depleted of mtDNA via treatment with ethidium bromide (EB) were found to contain reduced stea

112 abeled with fluorescein amidite (FAM-ssDNA), ethidium bromide (EB), and graphene oxide (GO) are emplo

113 complexation with the phenanthridinium drug ethidium bromide (EB).

114 solutions by monitoring the amount of bound Ethidium Bromide (EB).

115 r previously by the intraspinal injection of ethidium bromide (EB).

116 TM4(85-105) sequence inhibits Hsmr-mediated ethidium bromide efflux from bacterial cells.

117 Because ethidium bromide efflux is an energy-dependent process a

118 The procedure developed here using bacterial ethidium bromide efflux pump activity may be a useful co

119 tions of the intercalative drugs, except for ethidium bromide, enhance production of topoisomerase--D

120 ed field gel electrophoresis (PFGE) and CsCl/ethidium bromide equilibrium centrifugation demonstrates

121 We utilize ethidium bromide (EtBr) as a model intercalator to demon

122 ine) lipids was quantified by an increase of ethidium bromide (EtBr) fluorescence.

123 tween genomic DNA and the intercalating drug ethidium bromide (EtBr) have been determined by use of a

124 Zn(2+) complexation inhibited ethidium bromide (EtBr) intercalation and stabilized FdU

125 al effects of binding the intercalating drug ethidium bromide (EtBr) to 160 base pair (bp) fragments

126 vo mtDNA polymerase activity assay utilizing ethidium bromide (EtBr) to deplete mtDNA, showed that po

127 hemichannel activity as evident by enhanced ethidium bromide (EtBr) uptake that could be blocked by

128 ility and a decreased ability to intercalate ethidium bromide (EtBr).

129 Currently, ethidium bromide (EthBr) is the cheapest and most used D

130 potential of several dyes [acridine orange, ethidium bromide, ethidium homodimer, bis-benzimide (DAP

131 as determined by mitochondrial function and ethidium bromide exclusion, was not inhibited by the bro

132 Gel electrophoresis and ethidium bromide experiments showed that 9a-9c associate

133 /mL culture produced significantly increased ethidium bromide fluorescence compared to nonexposed con

134 Ethidium bromide fluorescence intensities increased upon

135 roxide anion production was measured with an ethidium bromide fluorescence method.

136 tocol to quantify PCR products, by measuring ethidium bromide fluorescence of PCR products excised fr

137 Ethidium bromide fluorescence of the product DNA was use

138 measured by lucigenin chemiluminescence and ethidium bromide fluorescence) and impaired endothelium-

139 (measured by lucigenin chemiluminescence and ethidium bromide fluorescence) that was inhibited or red

140 s of A vessels produced O(2)(.-) (shown with ethidium bromide fluorescence).

141 Data from circular dichroism, inhibition of ethidium bromide fluorescence, interstrand cross-linking

142 DNA cross-linking, assayed by ethidium bromide fluorescence, was significantly inhibit

143 en the amplified products were visualized by ethidium bromide fluorescence.

144 polyamines retain their ability to displace ethidium bromide from calf thymus DNA and are rapidly ta

145 se polyamines retain the ability to displace ethidium bromide from calf thymus DNA and are rapidly ta

146 It displaces 50% of ethidium bromide from DNA at a charge ratio (+/-) of 0.9

147 grouped depending on whether they displaced ethidium bromide from DNA.

148 ed with Southern blot analysis compared with ethidium bromide gel electrophoresis (EtBr) for all mRNA

149 ed with PCR analysis as a 173-bp fragment on ethidium bromide gels.

150 and the bound plasmids were eluted using an ethidium bromide gradient.

151 These observations suggest that ethidium bromide has a preferred intercalation site clos

152 gels with the binding of the fluorescent dye ethidium bromide has been a basic experimental technique

153 Ethidium bromide has served as a classic DNA intercalato

154 drugs including echinomycin, actinomycin-D, ethidium bromide, Hoechst 33342, and cis-C1 were subject

155 Uptake of ethidium bromide (i) was faster in Cx43 and Cx43-EGFP th

156 rsed both tolerance to INH and resistance to ethidium bromide in BCG.

157 p inhibitor reserpine inhibits resistance to ethidium bromide in both wild-type M. smegmatis and the

158 Inclusion of ethidium bromide in the reaction mixture leads to a grea

159 An insertion mutant of the ethidium bromide-induced all7631 did not show any signif

160 lt rat sciatic nerves into X-irradiation and ethidium bromide-induced demyelinated dorsal column lesi

161 e investigated the effect of previous focal, ethidium bromide-induced demyelination of brain stem whi

162 Following ethidium bromide injection there was a decrease in secur

163 stranded DNA with hybridization detected via ethidium bromide intercalation, further establishing tec

164 Titration of ethidium bromide into the assay decreased activity to a

165 We used injections of the gliotoxin ethidium bromide into the dorsal funiculus of the cervic

166 is responsible for drug resistance and that ethidium bromide is a novel substrate for P55.

167 x and 543-nm excitation for the detection of ethidium bromide-labeled nucleic acids (i.e., RNA).

168 ed fluorescence (LIF) was employed to detect ethidium bromide-labeled RNA molecules under native cond

169 lls were transplanted into the X-irradiation/ethidium bromide lesioned dorsal columns of immunosuppre

170 embrane permeable DNA-associating vital dye, ethidium bromide monoacetate (visible wavelength single

171 Ethidium bromide no longer intercalates between base pai

172 cted by staining with either acridine orange/ethidium bromide or annexin-V-fluorescein/propidium iodi

173 ignals that could be reversed by addition of ethidium bromide or by DNA melting, suggesting that flav

174 of the protein association to treatment with ethidium bromide or micrococcal nuclease.

175 ed protein response in wild-type worms using ethidium bromide or paraquat triggered statin resistance

176 Fluorescence-based binding assays that use ethidium bromide or Rev peptide displacement are used to

177 Twisting was controlled using ethidium bromide or SYBR Green I as model intercalators.

178 luorescence derived from the displacement of ethidium bromide or thiazole orange from the DNA of inte

179 Animals received ethidium bromide plus photon irradiation producing discr

180 rophoresis in agarose gels and staining with ethidium bromide, produced DNA fragments in the 4.0- to

181 Films treated with ethidium bromide prompt switching of dsDNA to ssDNA befo

182 Further, acridine orange, ethidium bromide, propidium iodide and DAPI staining dem

183 g a DNA-targeting intercalating agent (i.e., ethidium bromide) resulted in a marked shift of the clea

184 tion between caffeine and acridine orange or ethidium bromide results in singlet-state lifetime incre

185 erine and palmatine and the DNA intercalator ethidium bromide, revealed a change in the absorbance an

186 Ethidium bromide selectively intercalates into the accep

187 caffeine and the nonplanar DNA intercalator ethidium bromide show optical shifts and steady-state fl

188 nes were confirmed as Salmonella specific on ethidium bromide-stained agarose gels by Southern hybrid

189 electrophoresis and uv transillumination of ethidium bromide-stained agarose gels we and others have

190 by BrdU uptake and cell counts of calcein AM/ethidium bromide-stained cells.

191 by visualizing 1.1- to 1.2- kb PAN RNA in an ethidium bromide-stained gel from poly(A)-selected RNA.

192 The ethidium bromide-stained gels are photographed or scanne

193 ) stain, confocal fluorescence microscopy of ethidium bromide-stained sections, electron microscopy,

194 transcription-PCR amplification followed by ethidium bromide staining (PCR-ETBr) or nucleic acid hyb

195 on of microsatellite changes: (a) silver and ethidium bromide staining of polyacrylamide gels; (b) ra

196 The results of gel electrophoresis and ethidium bromide staining of the DNA fingerprints obtain

197 n to orange/yellow shifts on acridine orange/ethidium bromide staining, and cell surface annexin V bi

198 s of total DNA in an agarose gel followed by ethidium bromide staining, and subsequent scanning of th

199 as 100 viable trophozoites as determined by ethidium bromide staining, while no signal was obtained

200 sualized after separation in agarose gels by ethidium bromide staining.

201 ll apoptosis as evidenced by acridine orange-ethidium bromide staining.

202 sis on 2 % agarose gels, and visualized with ethidium bromide staining.

203 aphy, SYBR Gold stain is more sensitive than ethidium bromide, SYBR Green I stain, and SYBR Green II

204 be detected with intercalating dyes such as ethidium bromide through a loss in the UV absorption sig

205 Binding of ethidium bromide to Escherichia coli tRNAVal and an RNA

206 ma COLO 16 cells were chronically exposed to ethidium bromide to inhibit mitochondrial DNA synthesis

207 Further studies using acridine orange and ethidium bromide to measure apoptosis revealed that mdr1

208 Binding ethidium bromide to one of these RNA fragments, which wo

209 Strikingly, both ethidium bromide transport and normal cell surface prope

210 se chain reaction of isolated total RNA from ethidium-bromide-treated and untreated cells.

211 educed mitochondrial DNA (mtDNA) contents by ethidium bromide treatment or myocytes treated with know

212 Reduction of mtDNA content in DRHEp2 by ethidium bromide treatment reduced the resistance.

213 everal mtDNA forms after severe depletion by ethidium bromide treatment showed that replication and m

214 that were rendered respiration deficient by ethidium bromide treatment.

215 Ethidium bromide uptake assays revealed increased envelo

216 Ethidium Bromide uptake in purified F4/80+/CD11b+ mdx ma

217 urrent, and, when expressed in HEK293 cells, ethidium bromide uptake was only approximately 5% that o

218 as indicated by BzATP-mediated Ca2+ influx, ethidium bromide uptake, and lactate dehydrogenase relea

219 nfrequent or brief opening could account for ethidium bromide uptake.

220 lity of pgs1Delta to grow in the presence of ethidium bromide was due to defective cell wall integrit

221 Ethidium bromide was used as the intercalating dye for l

222 proteins during recovery from treatment with ethidium bromide, when mtDNA replication is stimulated i

223 e to treatment of the organelles with ATP or ethidium bromide, which affects differentially the rates

224 Ethidium bromide, which binds reversibly to DNA via inte

225 elination in rodent CNS in the X-irradiation/ethidium bromide (X-EB) model.

226 emyelinating lesions had been produced using ethidium bromide/X-irradiation.