ライフサイエンスコーパス: exonucleolytic

1 e endonucleolytic DNA incisions, followed by exonucleolytic 3' -> 5' degradation of the individual DN

2 components of the 60S ribosomal subunit, and exonucleolytic 5' processing of 5.8S rRNA was strongly i

3 unction with various metal ions at the 3'-5' exonucleolytic active site of the Klenow fragment (KF) o

4 e-stranded DNA substrates bound to the 3'-5' exonucleolytic active site of the large fragment of DNA

5 Eukaryotic 3'-->5' exonucleolytic activities are essential for a wide varie

6 MRN catalyzes sequential endonucleolytic and exonucleolytic activities on both 5' and 3' strands of D

7 Degradation by exonucleolytic activities was temperature sensitive in e

8 replication, relying on endonucleolytic and exonucleolytic activities, respectively.

9 and junction-specific endonuclease and ssDNA exonucleolytic activities.

10 ifunctional RNases possessing both endo- and exonucleolytic activities.

11 h has a key role in determining endo- versus exonucleolytic activity across the SNM1 family.

12 ease, encoded by the smx gene, that exhibits exonucleolytic activity and is regulated as part of the

13 They possess both 5'-3' exonucleolytic activity and the ability to cleave bifurc

14 activity at branched DNA structures and its exonucleolytic activity at nick and gap structures.

15 al NF-kappaB pathway is essential for normal exonucleolytic activity during human V(D)J recombination

16 merases from the A and B families with 3'-5' exonucleolytic activity have exonuclease domains with si

17 e consequence of endonucleolytic or 5'-to-3' exonucleolytic activity is unclear.

18 A model explaining the exonucleolytic activity of FEN-1 in terms of its endonuc

19 teins define termini by blocking the 5'-->3' exonucleolytic activity of ribonuclease J (RNase J).

20 The assay exploits the 5'-->3' exonucleolytic activity of Taq DNA polymerase to increas

21 eam of either primer during PCR, the 5'-->3' exonucleolytic activity of Taq polymerase degrades it, a

22 The role of the exonucleolytic activity of the calf 5' to 3' exo/endonuc

23 P-1 may play a role in the regulation of the exonucleolytic activity of WRN.

24 hFEN1 endonucleolytic activity versus hEXO1 exonucleolytic activity on 5'-flap substrates.

25 s blocked by a terminal dU; there was slight exonucleolytic activity on a 3'-terminal A or C but no a

26 because reverse transcriptases (RT) lack an exonucleolytic activity that can remove incorporated nuc

27 ro with synthetic RNAs, displays both 5'->3' exonucleolytic activity, as well as robust endonucleolyt

28 n DNA polymerase beta, which lacks intrinsic exonucleolytic activity, primer extension proceeds to th

29 a potent 3' to 5' single-strand DNA-specific exonucleolytic activity.

30 lacking the nuclease domain does not display exonucleolytic activity.

31 s of these RNAs and protects them against 3'-exonucleolytic activity.

32 tary nucleotides using Kpom1 lacking 3'-->5' exonucleolytic activity.

33 nation intermediates are substrates for both exonucleolytic and 5' flap endonucleolytic cleavage.

34 Human FEN1 exhibits both a 5' to 3' exonucleolytic and a structure-specific endonucleolytic

35 e demonstrated the presence of both 5'-to-3' exonucleolytic and endonucleolytic activities on the Bac

36 1 is a dual-specificity enzyme, with both 5' exonucleolytic and endonucleolytic activities.

37 alent cation requirements and possessed both exonucleolytic and endonucleolytic functions.

38 n EXO-1 and dramatically stimulates both the exonucleolytic and endonucleolytic incision functions of

39 added to the assay, the balance between the exonucleolytic and endonucleolytic modes of hydrolysis s

40 Exonucleolytic and fork-gap-endonucleolytic reactions we

41 e rRNAs may provide a buffer zone against 3' exonucleolytic attack, thereby preserving the encoded se

42 yadenylate (polyA) tail to sequester it from exonucleolytic attack.

43 tein stimulates both the endonucleolytic and exonucleolytic cleavage activity of FEN-1 and this funct

44 from the primer-templates indicate that the exonucleolytic cleavage activity of Klenow fragment is c

45 substrate for Taq DNA polymerase only after exonucleolytic cleavage had destabilized the hairpin.

46 cleavage of RNA by FttA, followed by 5'->3' exonucleolytic cleavage of RNA by FttA and concomitant 5

47 rophosphorolysis as well as in the endo- and exonucleolytic cleavage of the nascent RNA.

48 th RNA chain extension and RNA shortening by exonucleolytic cleavage or pyrophosphorolysis and increa

49 cular principles governing diverse endo- and exonucleolytic cleavage specificities of members of the

50 h on the ability of HMO2 to protect DNA from exonucleolytic cleavage suggests that more than one HMO2

51 markable ability of HMO2 to protect DNA from exonucleolytic cleavage, combined with reports that HMO2

52 hat NGD primarily proceeds via Xrn1-mediated exonucleolytic decay and Cue2-mediated endonucleolytic d

53 leolytic cleavage are most likely removed by exonucleolytic decay as well, but these events have not

54 sing fluorescence and UV melts, FRET, and an exonucleolytic decay assay we define a concerted mechani

55 ified CspE impedes poly(A)-mediated 3' to 5' exonucleolytic decay by PNPase by interfering with its d

56 lp explain how deadenylation, decapping, and exonucleolytic decay can all be independently activated

57 These data indicate that 3'-5' exonucleolytic decay is the major pathway of RNA degrada

58 enylation, decapping and subsequent 5' to 3' exonucleolytic decay of the transcript body.

59 Furthermore, 3'-5' exonucleolytic decay was stimulated dramatically by AU-r

60 ibonucleotide fragments generated by 3'-->5' exonucleolytic decay, and cleavage of m(7)GDP generated

61 se mRNA decay processes, as well as 5'-to-3' exonucleolytic decay, associate with the protein tristet

62 sitive to structures or sequences that block exonucleolytic decay, is required for efficient decay of

63 resulting in mRNA deadenylation followed by exonucleolytic decay, mRNA endonucleolytic cleavage, or

64 to probe pathways of endonucleolytic versus exonucleolytic decay, were measured in an RNase Y-defici

65 activities are linked and there is a coupled exonucleolytic decay-dependent decapping pathway.

66 volving deadenylation and subsequent 3'-->5' exonucleolytic decay.

67 nylation, followed by decapping and 5' to 3' exonucleolytic decay.

68 provokes rapid decapping followed by 5'-->3' exonucleolytic decay.

69 some complex is required for efficient 3'-5' exonucleolytic decay.

70 uires deadenylation, decapping, and 5'-to-3' exonucleolytic decay.

71 n precedes decapping and subsequent 5'-to-3' exonucleolytic decay.

72 adenylation followed by 5' end decapping and exonucleolytic decay.

73 d for both 5'-to-3' and 3'-to-5' pathways of exonucleolytic decay.

74 tivate deadenylation, decapping, or 3'-to-5' exonucleolytic decay.

75 ly dominant, suggesting that they carried an exonucleolytic defect but retained binding to the pol II

76 antly inhibited both the endonucleolytic and exonucleolytic degradation activities, while deletion of

77 ntial service by protecting chromosomes from exonucleolytic degradation and end-to-end fusions and by

78 sistent with protection of the DNA ends from exonucleolytic degradation and repair by the c-NHEJ path

79 modification exposes transcripts to rapid 5' exonucleolytic degradation by RNase J, which is absent i

80 pyrophosphohydrolase RppH triggers rapid 5'-exonucleolytic degradation by RNase J.

81 The P-element termini were protected from exonucleolytic degradation following the cleavage reacti

82 ation is a widespread process in which 5'-3' exonucleolytic degradation follows the last translating

83 adenylation, subjects substrate RNAs to slow exonucleolytic degradation from the 3' end in vitro.

84 Cytoplasmic mRNA decay is effected by exonucleolytic degradation in either the 5' to 3' or 3'

85 tides upstream of the intron insertion site, exonucleolytic degradation is required for recombination

86 Processive 3'-to-5' exonucleolytic degradation of an SP82 phage RNA substrat

87 Nbs1 inhibits Mre11/Rad50-catalyzed 3'-to-5' exonucleolytic degradation of clean DNA ends.

88 Exonucleolytic degradation of DNA is an essential part o

89 caps the cap structure generated by 3' to 5' exonucleolytic degradation of mRNA.

90 f1p, Nmd2p, and Upf3p regulate decapping and exonucleolytic degradation of nonsense-containing mRNAs.

91 The kinetics for exonucleolytic degradation of single-stranded, paired, a

92 RecJ requires interaction with SSB for exonucleolytic degradation of ssDNA but not dsDNA.

93 ivates the mRNA and that this is followed by exonucleolytic degradation of the cleavage products.

94 The resulting exonucleolytic degradation of the primer serves to move

95 n yeasts was shown to be lethal due to rapid exonucleolytic degradation of uncapped transcripts or fa

96 ction in cell extracts, which occurred by 3'-exonucleolytic degradation rather than endonucleolytic f

97 by promoting such rapid RNA cleavage and 5' exonucleolytic degradation that PPR10 had insufficient t

98 karyotic gene expression and often relies on exonucleolytic degradation to eliminate dysfunctional tr

99 nstream cleavage product, protecting against exonucleolytic degradation, and thereby limiting the ext

100 Since these ends are subject to exonucleolytic degradation, the assay may demand rapid r

101 eep-sequencing method to measure chromosomal exonucleolytic degradation, we demonstrate that the abse

102 nslation and protects the transcript against exonucleolytic degradation.

103 ion-dependent decapping followed by 5' to 3' exonucleolytic degradation.

104 pping that exposes the transcript to 5'-->3' exonucleolytic degradation.

105 thereby exposing the mRNA to rapid 5' to 3' exonucleolytic degradation.

106 ff the 5' cap to leave an end susceptible to exonucleolytic degradation.

107 ng, which exposes the transcript to 5' to 3' exonucleolytic degradation.

108 tion that exposes the transcript to 5' to 3' exonucleolytic degradation.

109 ss of deadenylation, decapping and 5' --> 3' exonucleolytic degradation.

110 ng, which exposes the transcript to 5' to 3' exonucleolytic degradation.

111 plex, which protected these transcripts from exonucleolytic degradation.

112 ed, antisense transcription-controlled 3'-5' exonucleolytic degradation.

113 and protects the mature 3'-end of TER1 from exonucleolytic degradation.

114 elomerase to the 3'-end and protects against exonucleolytic degradation.

115 he 5' end of nascent mRNA to protect it from exonucleolytic degradation.

116 s induced by DNA topoisomerase II, including exonucleolytic deletion and template-directed polymeriza

117 also participates in RNA degradation through exonucleolytic digestion and polyadenylation.

118 ow that the Ku complex shields DNA ends from exonucleolytic digestion but facilitates endonucleolytic

119 by the Mre11-Rad50-Xrs2 complex (MRX), then exonucleolytic digestion by Exo1.

120 e ability of damaged nucleo-tides to inhibit exonucleolytic digestion differs significantly between W

121 beta signal joints, and from N additions and exonucleolytic digestion for TCR-delta joints.

122 th defective helicase activity are active in exonucleolytic digestion of DNA.

123 ysis of chromosome fusion junctions revealed exonucleolytic digestion of dysfunctional ends prior to

124 hat progresses from removal of the 5' cap to exonucleolytic digestion of the body of the mRNA.

125 eadenylation followed by decapping and 5'-3' exonucleolytic digestion of the mRNA.

126 pre-microRNA/Exp5/Ran-GTP complex inhibited exonucleolytic digestion of the pre-miRNA in vitro.

127 Here we demonstrate requirements for exonucleolytic digestion of unpaired 3' tails before pol

128 the 5' cap structure (decapping) and 5'->3' exonucleolytic digestion, or by 3' to 5' degradation.

129 sence of RNase A, but not RNase H, inhibited exonucleolytic digestion, suggesting that a ribonucleopr

130 removal of the cap structure permits 5'-->3' exonucleolytic digestion.

131 denylation followed by decapping and 5'-->3' exonucleolytic digestion.

132 decay intermediate is generated by 5'-to-3' exonucleolytic digestion.

133 functional complex and create substrates for exonucleolytic digestion.

134 tion which exposed the transcript to 5'-->3' exonucleolytic digestion.

135 -3': XY = GG, GA, AG, GU) are found to block exonucleolytic digestion.

136 degraded through either 5' to 3' or 3' to 5' exonucleolytic digestion.

137 d in the cytoplasm by decapping and 5'-to-3' exonucleolytic digestion.

138 mbination and protecting genomic DNA against exonucleolytic DNA degradation.

139 berrant recombination and protecting against exonucleolytic DNA degradation.

140 The HNH active site of Cas9 catalyzes exonucleolytic DNA trimming by a mechanism that is indep

141 butions of Nbr's N-terminal domain (NTD) and exonucleolytic domain (EXO) in miRNA 3'-end trimming.

142 We also provide a genome-wide analysis of exonucleolytic DSB resection lengths and elucidate spati

143 Exonucleolytic editing of incorrectly incorporated nucle

144 Thus, exonucleolytic end resection, rather than chromosome fus

145 n and recruits decapping, deadenylation, and exonucleolytic enzymes to PBs for RNA turnover.

146 and the nuclear exosome, a large complex of exonucleolytic enzymes.

147 ely 50 bp) oligonucleotide substrates during exonucleolytic excision by the formation of a discontinu

148 ucleases (FENs) catalyze endonucleolytic and exonucleolytic (EXO) DNA hydrolyses.

149 on of Rad50 does not significantly alter the exonucleolytic function of Mre11.

150 ole of the PNPase-RNase Y interaction in the exonucleolytic function of PNPase needs to be clarified.

151 e motif selectively eliminated the 3' --> 5' exonucleolytic function of the purified mutant polymeras

152 This bidirectional exonucleolytic gap expansion ultimately promotes their c

153 the high mispair specificity of pol gamma in exonucleolytic hydrolysis is maintained, indicating that

154 Flap endonucleases (FENs) catalyse the exonucleolytic hydrolysis of blunt-ended duplex DNA subs

155 2)P at the 5' or 3' ends, *AN/L3 carried out exonucleolytic hydrolysis of both substrates exclusively

156 eled RNA in a manner consistent with a 3'-5' exonucleolytic mechanism.

157 a substrate recruitment platform to execute exonucleolytic miRNA maturation, catalyzed by the ribonu

158 lls confirmed a principal role of the NTD in exonucleolytic miRNA trimming, which depends on basic su

159 ning alternate secondary structures, with an exonucleolytic mode of action suggestive of RNaseD.

160 response factor 1, proteins that facilitate exonucleolytic mRNA, to exit SGs.

161 c pathway rather than the SMG5-SMG7-mediated exonucleolytic pathway.

162 ly(A) tails by what has been described as an exonucleolytic process that can be blocked by the presen

163 ved a coding-partner-dependent difference in exonucleolytic processing and an age-specific difference

164 tional mutagenesis in S.pombe is preceded by exonucleolytic processing and concatomerization of the t

165 P cleavage, while the 3' terminus undergoes exonucleolytic processing by a combination of 3' --> 5'

166 This was followed by 3' to 5' exonucleolytic processing by RRP6 and the exosome, an en

167 the 11-subunit cellular noncoding RNA 3'-5' exonucleolytic processing complex RNA exosome.

168 The subsequent exonucleolytic processing is carried out largely by RNas

169 In addition, the extent of exonucleolytic processing of coding ends was inversely r

170 Our results suggest that exonucleolytic processing of primary DNA lesion by hRAD9

171 Because models predict exonucleolytic processing of the cleaved recipient leadi

172 stream from the mature terminus, followed by exonucleolytic processing to a stem-loop within the 3'-u

173 rucei, mitochondrial pre-mRNAs undergo 3'-5' exonucleolytic processing, 3' adenylation and uridylatio

174 s by avoiding the widespread use of 3' -> 5' exonucleolytic processing, 3'-polyadenylation and subseq

175 eterogeneous 5' termini that may result from exonucleolytic processing, and occasionally robust decap

176 nascent transcript and protect it from 3'-5' exonucleolytic processing.

177 ctions, unifying the mechanisms of endo- and exonucleolytic processing.

178 With poly(dA):oligo(dT)50 as substrate, the exonucleolytic products formed a continuous ladder with

179 DNA polymerase beta has no exonucleolytic proof-reading ability, and exhibits high

180 cherichia coli dnaQ gene encodes the 3'-->5' exonucleolytic proofreading (epsilon) subunit of DNA pol

181 ranscriptase (RT) does not possess 3'- to 5'-exonucleolytic proofreading activity and because RT has

182 tes are removed by DNA polymerase-associated exonucleolytic proofreading activity and/or the postrepl

183 (epsilon) subunit of HE provides the 3'-->5' exonucleolytic proofreading activity for this complex.

184 on subunit of the HE complex provides the 3'-exonucleolytic proofreading activity for this enzyme com

185 h repair (MMR) machinery, rather than by the exonucleolytic proofreading activity of DNA polymerase.

186 has been hypothesized to enhance the 3'-->5' exonucleolytic proofreading activity of epsilon.

187 delta variants that harbor or lack 3' --> 5'-exonucleolytic proofreading activity were purified from

188 erichia coli DNA polymerase III possesses 3'-exonucleolytic proofreading activity.

189 Exonucleolytic proofreading and DNA mismatch repair (MMR

190 replication errors that are subject to both exonucleolytic proofreading and dnaE antimutator effects

191 fidelity, even in the absence of its 3'-->5' exonucleolytic proofreading and is significantly more ac

192 he corresponding frameshift intermediates by exonucleolytic proofreading and/or mismatch repair.

193 cribe the engineering of XNA RTs with active exonucleolytic proofreading as well as the directed evol

194 We suggest that the normal level of exonucleolytic proofreading associated with the mutant P

195 dium, where the mutD5 strain is defective in exonucleolytic proofreading but has a functional MMR sys

196 on by mammalian terminal transferase, blocks exonucleolytic proofreading by Escherichia coli DNA poly

197 Thus, both polymerase selectivity and exonucleolytic proofreading efficiency are diminished du

198 The influence of mutations in the 3' to 5' exonucleolytic proofreading epsilon-subunit of Escherich

199 the polymerase domain is more critical than exonucleolytic proofreading for the fidelity of pol I in

200 d in vivo by mutations that eliminate the 3'-exonucleolytic proofreading function.

201 merase, and the epsilon subunit contains the exonucleolytic proofreading function.

202 ce of these analogs in DNA and inhibition of exonucleolytic proofreading may also contribute to mitoc

203 ort the model that the rate-limiting step in exonucleolytic proofreading of DNA by epsilon subunit is

204 test the hypothesis that the contribution of exonucleolytic proofreading to frameshift fidelity durin

205 ce nucleotide selectivity, the efficiency of exonucleolytic proofreading, and the rate of forming err

206 ns of polymerase base selectivity, 3' --> 5' exonucleolytic proofreading, mismatch correction, and DN

207 to increase genome instability by disrupting exonucleolytic proofreading, the P286R variant was later

208 pe pol gamma despite the action of intrinsic exonucleolytic proofreading.

209 results from high nucleotide selectivity and exonucleolytic proofreading.

210 Both effects are classical hallmarks of exonucleolytic proofreading.

211 s are present to counteract the effect of 3'-exonucleolytic proofreading.

212 We show for the first time that the exonucleolytic Rat1.Rai1 complex can elicit the release

213 he enzymes that carry out the early 5' to 3' exonucleolytic reactions that generate the mature rRNAs.

214 e snoRNA genes and their processing involves exonucleolytic release of the snoRNA from debranched int

215 he editing site followed by U-specific 3'-5'-exonucleolytic removal of nonbase-paired Us prior to lig

216 e (pol gamma) by comparing the insertion and exonucleolytic removal of six antiviral nucleotide analo

217 e presence of dNTP and to a lesser extent to exonucleolytic removal of the 3'-phosphate-bearing termi

218 inal C (producing a base substitution), (ii) exonucleolytic removal of the C, or (iii), for the G-con

219 DNA-PK, Artemis catalyzed extensive 5'-->3' exonucleolytic resection of double-stranded DNA.

220 Exonucleolytic resection, critical to repair double-stra

221 o a futile cycle of abortive TLS followed by exonucleolytic reversal.

222 anscriptional RNA cleavage events, and 5'-3' exonucleolytic RNA degradation in the mammalian Pol II t

223 the potential of protocols incorporating an exonucleolytic snake venom phosphodiesterase (SVPD) dige

224 al processing requires a series of endo- and exonucleolytic steps for the production of mature riboso

225 by AGO4 and subsequently subjected to 3'-5' exonucleolytic trimming for maturation.

226 Has1 binding triggers exonucleolytic trimming of 27SA3 pre-rRNA to generate th

227 7p is specifically required for the 5' to 3' exonucleolytic trimming of the 27SA(3) into the 27SB(S)

228 piRNA 3' ends are probably formed by exonucleolytic trimming, after a piRNA precursor is load

229 sors by endonucleolytic cleavage followed by exonucleolytic trimming.

230 ase as 3' extended precursors, which undergo exonucleolytic trimming.

231 B. subtilis, a long-standing question on the exonucleolytic versus endonucleolytic nature of 16S rRNA