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1 were only present in co-cultures with mouse feeder cells.
2 ing in the absence of exogenous cytokines or feeder cells.
3 m in the presence of mitomycin C-treated 3T3 feeder cells.
4 ed and used to generate embryonic fibroblast feeder cells.
5 rum in the presence of mitomycin-treated 3T3 feeder cells.
6 stead of IL-2, in the presence of engineered feeder cells.
7 liminates the requirement for animal-derived feeder cells.
8 xtension to human iPS cells cultured without feeder cells.
9 d for expanding these cells in vitro without feeder cells.
10 lated NGFR-expressing cells were free of PA6 feeder cells.
11 le factor (or factors) released by apoptotic feeder cells.
12 ot lead to immortalization in the absence of feeder cells.
13 without the need for supporting mesenchymal feeder cells.
14 tin (perlecan, fibulin-2), in the absence of feeder cells.
15 ated with radiation-induced apoptosis of the feeder cells.
16 e Notch ligand Dll4 on hESC-derived vascular feeder cells.
17 with cytokines alone or in combination with feeder cells.
18 B-CLL cells died after removal of macrophage feeder cells.
19 rent to human stem-cell xenoculture on mouse feeder cells.
20 nating potential variability caused by using feeder cells.
21 genitors through coculture of hESCs with OP9 feeder cells.
22 ith Jagged-1 knockdown mesothelial and tumor feeder cells.
23 occurred in both the absence and presence of feeder cells.
24 oribosyltransferase (Hprt) gene and grown on feeder cells.
25 culture conditions with embryonic fibroblast feeder cells.
26 onic bodies in the absence of murine stromal feeder cells.
27 reactive proliferative response to syngeneic feeder cells.
28 d expanded by mitogen and IL-2 on allogeneic feeder cells.
29 the infected cells were grown on fibroblast feeder cells.
30 unction derived from autologous or xenogenic feeder cells.
32 varian cancer cells with Jagged-1-expressing feeder cells activated the promoter activity of candidat
35 yos were transplanted onto neonatal cortical feeder cells and assessed for their ability to generate
38 monstrated that physical contact between the feeder cells and keratinocytes is not required for induc
40 The combination of irradiated fibroblast feeder cells and Rho kinase inhibitor, Y-27632, conditio
43 tory culture without the use of heterologous feeder cells and their viability was demonstrated in viv
44 nued cell proliferation is dependent on both feeder cells and Y-27632, and the conditionally reprogra
45 and anti-CD28 monoclonal antibodies, CD4(+) feeder cells, and interleukin 2, provided for marked exp
46 crodrops seeded with mitotically inactivated feeder cells, and then connected with neighboring microd
47 e been established decades ago, a long-term, feeder cell- and serum-free culture system recapitulatin
48 major advantages of senescent fibroblasts as feeder cells are (i) the need to establish primary cultu
50 n in co-culture with post-mitotic fibroblast feeder cells as compared with keratinocytes grown on tis
51 gene-corrected cells were obtained free from feeder cells at a "purity" of >30%, enriched >2,000-fold
53 otoxicity in hypoxia, but preactivation with feeder cells bearing IL-21 and 4-1BBL was even better.
54 s that differentiate in this time or require feeder cells, because the feeders must be drug resistant
55 kines IL-2, IL-21 and irradiated 3T3-msCD40L feeder cells can successfully stimulate switch-memory B
56 cient source of autologous iPS cells and, as feeder cells, can also maintain iPS and ES cell pluripot
57 ematopoietic progenitors was determined in a feeder cell coculture system and assayed by quantitating
58 d by using various empirical combinations of feeder cells, conditioned media, cytokines, growth facto
61 nic stem cells (ES cells) in vitro depend on feeder cell-derived growth factors that are largely unid
63 hat keratinocytes cultured in the absence of feeder cells exhibit a migratory phenotype and suggest t
64 mal differentiation was triggered on stromal feeder cells followed by regional specification by means
65 tocol also describes how ASCs can be used as feeder cells for maintenance of other pluripotent stem c
66 th ESCs grown on feeder cells, ESCs grown in feeder cell-free conditions exhibited decreased immunosu
67 em cell-based, chemically-defined, serum and feeder cell-free culture system, we show that the AhR is
68 y human B cell progenitors, we established a feeder cell-free in vitro system allowing the developmen
69 Here we report establishment of an in vitro feeder-cell-free LSC expansion and three-dimensional cor
70 ith other methods, our protocol does not use feeder cells, has a minimum dependence on proteins (purm
71 inocytes grown in co-culture with fibroblast feeder cells have an extended in vitro lifespan and dela
74 urified progenitors were plated onto stromal feeder cells in the presence of a large excess of differ
75 nitial TCR stimulation, but neither the PBMC feeder cells in the REP or the activated TIL expressed 4
77 or (Y-27632), in combination with fibroblast feeder cells, induces normal and tumor epithelial cells
78 ures from experimental animals for preparing feeder cells is obviated; (ii) the risk of contamination
82 ce of interleukin-2 (IL-2) and an allogeneic feeder cell layer, or IL-2 and other hematopoietic growt
83 ansion in vitro in the absence of serum or a feeder cell layer, suggesting that additional signals ar
86 d) transgenic fish using a zebrafish ovarian feeder cell line (OFC3) that was engineered to express z
90 of the cell culture system was the use of a feeder cell line that was initiated from ovaries of a tr
93 ined culture conditions and the avoidance of feeder cells or embryoid bodies allowed synchronous and
94 ES) cells rely on growth factors provided by feeder cells or exogenously to maintain their pluripoten
96 ccinated donors, we optimized SCCs with NB21 feeder cells, R848, and IL-2, achieving efficient clonal
99 us telomerase expression and co-culture with feeder cells results in efficient extension of lifespan
101 hematopoietic precursors in vitro use either feeder cell, serum, conditioned culture medium or embryo
102 mically defined conditions in the absence of feeder cells, serum, and leukemia inhibitory factor.
103 gers-which we collectively refer to as cross-feeder cells-slowed down the growth of degrader cells.
104 e MEF and human placental stromal fibroblast feeder cells, some proteins were only expressed in suppo
105 activity alone when grown in co-culture with feeder cells, suggesting that loss of the p16(INK4a)/Rb
106 growth in culture, whether in the fibroblast feeder cell system or in the specialized K-sfm medium fo
107 induce pluripotency, and requires the use of feeder cells that add complexity and variability to the
108 ated every 2 weeks with irradiated alogeneic feeder cells, that had similar functional properties thu
109 ep for 1 month were transferred onto Sertoli feeder cells, they differentiated into functional sperm
110 ly immunodeficient children were cultured on feeder cells, they well supported R5, but not X4 HIV-1 r
113 LSC expansion setting, we generated NIH-3T3 feeder cells to overexpress different levels of Wnt6.
114 are then dispersed and plated on irradiated feeder cells to propagate and isolate individual iPSC cl
115 m cell typically rely on protein matrices or feeder cells to support attachment and growth, while mec
116 helial cells cultured in the presence of 3T3 feeder cells undergo biochemical differentiation, as evi
117 d colonies separated from one another by the feeder cells were detached as a sheet from the dish and
118 ree culture system, devoid of animal-derived feeder cells, were sorted by relative cell size and char
119 actors, we have established sub-lines of STO feeder cells which exhibit variable ability in supportin
120 ves coculture of irradiated mouse fibroblast feeder cells with normal and tumor human epithelial cell