ライフサイエンスコーパス: filament

1 reversible rotary motor at the base of each filament.

2 Ca(2+)-saturated states of the cardiac thin filament.

3 subsequent incorporation into the archaellum filament.

4 n of discrete defects in the nanometer-sized filament.

5 tely leading to full decoration of the actin filament.

6 large macromolecular assembly like the thin filament.

7 d attached to the end of the nucleated actin filament.

8 1-RAD51 interaction within the nucleoprotein filament.

9 including the rearrangement of cytoskeletal filaments.

10 ctile activity of sparsely distributed NMIIA filaments.

11 requires forces generated by MYO7B and actin filaments.

12 ort, initiated by nearby elongation of actin filaments.

13 anical stimulation of the face with Von Frey filaments.

14 ar and, for smooth muscle myosin, side-polar filaments.

15 ular framework regulating the behavior of MT filaments.

16 en by generic physical forces acting between filaments.

17 fragment that remains associated with thick filaments.

18 ks by stimulating branching from preexisting filaments.

19 anch junction and its interaction with actin filaments.

20 ed, contain rod bodies, and have longer thin filaments.

21 by binding to and moving along actin of thin filaments.

22 5-nanometer-long threefold-symmetric amyloid filaments.

23 uggesting that both proteins stabilize actin filaments.

24 tin, thereby inhibiting assembly of vimentin filaments.

25 bules, and cytokeratin 19-based intermediate filaments.

26 skin cells contained both actin and keratin filaments.

27 ric particles, which must polymerize to form filaments.

28 tabilized zirconia (YSZ), toward eliminating filaments.

29 n with rays highly modified into specialized filaments.

30 of protein synthesis, into large bundles of filaments.

31 ies to disseminate compared with long hyphal filaments.

32 at promotes polymerization of branched actin filaments.

33 A-ATPases that cut microtubules into shorter filaments.

34 ich both 3R and 4R isoforms are found in the filaments)(16).

35 ccurs at early times in both femtosecond and filament ablation plumes, although with different tempor

36 motor detaches from the track and binds the filament again in the leading position.

37 thick filaments that organized into complex filament aggregates and fibrillar sheets.

38 s that conformational characteristics of tau filaments, along with regional vulnerability to tau path

39 associated with the thick, myosin-containing filament and exhibits a complex pattern of immunoglobuli

40 ssential for substrate channeling within the filament and for the regulation of enzyme activity.

41 ted end is flatter than the remainder of the filament and has a conformation distinct from G-actin, m

42 ion and extension of the RAD51 nucleoprotein filament and that the entropic penalty associated with r

43 e role of titin as a blueprint for the thick filament and the arrangement of the myosin motor domains

44 stable annealing between the recombinogenic filament and the donor template in yeast, limiting stran

45 creasing dwell times of individual myosin II filaments and a global change from a remodeling to a con

46 tin cytoskeleton, a dynamic network of actin filaments and associated F-actin-binding proteins, is fu

47 and disassembly kinetics of Tpm1.8 on single filaments and at the single-molecule level.

48 a are propelled by rigid, helical, flagellar filaments and display distinct swimming patterns to expl

49 In vivo, colocalization of actin filaments and divalent ions are suppressed, and cells re

50 ruitment of mDia1 to SPIN90-Arp2/3 nucleated filaments and formation of a ternary SPIN90-Arp2/3-mDia1

51 in the simplifying context of cultured cell filaments and in protoplasts before and during regenerat

52 We find that SMTNL2 binds to actin filaments and is required to slow down the turnover of a

53 howed that direct interactions between actin filaments and lipid bilayers are possible and that the n

54 ts showed shortened sarcomeres with no thick filaments and M-lines in slow fibers of the mutant embry

55 arose independently from other actin-related filaments and may allow yeast to rapidly modulate glucok

56 We also performed the packing experiment of filaments and membranes to demonstrate the simulation ab

57 Bundles of cytoskeletal filaments and molecular motors generate motion in living

58 lationships among bacterial and archaeal T4P filaments and provide insights into archaeal virus-host

59 gest that the interactions between ESCRT-III filaments and the membrane could proceed through multipl

60 lipodia, depends on the barbed ends of actin filaments, and requires both the LIM domain and the nebu

61 with cytoplasmic giantin-based intermediate filaments, and such cells showed antiviral activity towa

62 Eye blinks evoked by von Frey filaments applied on the cornea were lower in Piezo2-def

63 ections, thus stabilizing those cytoskeletal filament architectures that result in shear-like pulling

64 We show that these filaments are coated by a highly asymmetric, multi-compo

65 king it difficult to determine whether actin filaments are directly associated with specific membrane

66 are well-captured by a model in which actin filaments are dynamically connected by a single dominant

67 ScDmc1 nucleoprotein filaments are less stable than the ScRad51 ones because

68 Filaments are regulated by actin-binding proteins, but t

69 We conclude that alpha-synuclein filaments are the main driving force for amplification a

70 Vertebrate striated muscle thin filaments are thought to be thermodynamically activated

71 works, and changes in microtubules and actin filaments are well studied.

72 n is Msp300/Nesprin-1, which organizes actin filaments around the new synapse.

73 they drive retrograde extension of an actin filament array that specifies anterograde microtubule po

74 ructurally and biochemically different actin filament arrays.

75 This establishes intact titin filaments as critical force-transmission networks, buffe

76 de in the field of spinning of nanocellulose filaments, as well as outline necessary steps for effici

77 Here we show that intermediate filaments assembled by keratins function as asymmetrical

78 uctural-mechanical characterization of lamin filaments assembled in situ remains elusive.

79 dynamical processes in cytoskeletal bundles: filament assembly and disassembly, the attachement-detac

80 ers, suggesting that the major place of thin filament assembly is an M-line-centered narrow domain wh

81 e, modulate cell shape by accelerating actin filament assembly locally and slowing filament capping.

82 ence that N-Wasp, a protein regulating actin filament assembly through Arp2/3 complex-dependent actin

83 ng which allowed us to determine that septin filament assembly was a diffusion-driven process, while

84 The structures define the mechanism of filament assembly, and reveal how filament-dependent all

85 is for the selectivity necessary for correct filament assembly.

86 M at the cell cortex and catalysed NM myosin filament assembly.

87 tion experimentally determined structures of filament-associated troponin are not available.

88 domains are regularly distributed along the filament at 4-nm intervals and we can determine the doma

89 desmin aggregates and the absence of desmin filaments at intercalated discs.

90 he interaction between the paired and single filaments at the outer zones of the transitional hourgla

91 This filament-based mechanism of enhanced cooperativity demon

92 opment, only about 10% of the motors in each filament bear the peak force, and these are confined to

93 nsion in the fused tube of petals and stamen filaments beneath the anther insertion point; by contras

94 e tropomyosin cable that fits onto the actin filament between the tip of the myosin head and a cleft

95 We propose that intrinsic properties of the filament-binding FH2 domain tune the efficiency of FH1-m

96 rces contribute to disassembling "old" actin filament branches in cells.

97 ion occurs before chemical adaptation during filament bundle formation: actin filaments first align i

98 whose coalescence was restricted by keratin filament bundles.

99 undling them and antagonized UNC-87-mediated filament bundling.

100 ding residues in FL villin that regulate its filament-bundling activity.

101 t divisome proteins follow treadmilling FtsZ filaments by a diffusion-and-capture mechanism, which ca

102 ICAL1, an enzyme known to depolymerize actin filaments by direct oxidation.

103 ASP), and contraction of the resultant actin filaments by myosin II.

104 n vitro Tau can be induced to form fibrillar filaments by oxidation of its two cysteine residues, gen

105 ro of nonphosphorylated smooth muscle myosin filaments by the addition of MgATP is the reverse of pol

106 est that polar and nematic patterns of actin filaments can interact and dynamically transform into ea

107 actin filament assembly locally and slowing filament capping.

108 vitro(1), as well as in live cells, in which filaments circle around the cell division site(2,3).

109 and highly twisted yarns made of continuous filaments, compared with those with a looser structure (

110 approaches to deduce global dynamics of thin filament components by energy landscape determination an

111 ns to scrutinize the structural details of a filament composed of Vps32 protomers, a major component

112 The core of a CBD filament comprises residues lysine 274 to glutamate 380

113 ing requires associations between other thin filament constituents.

114 ated, bound cofilin compromises longitudinal filament contacts of 1 protofilament, consistent with a

115 that, although myosin motors throughout the filament contribute to force development, only about 10%

116 Here, we used EM to show that actin filaments convert directly into globular material.

117 ment are identified and resolved in both the filament core and reservoir regions.

118 we present the cryo-EM structure of the UMOD filament core at 3.5 angstrom resolution, comprised of t

119 tic actin networks through rapid binding and filament crosslinking.

120 parasite plasma membrane and an intermediate filament cytoskeleton called the inner-membrane complex

121 chanism of filament assembly, and reveal how filament-dependent allosteric regulation of IMPDH2 makes

122 Here we show that keratin intermediate filaments directly regulate the morphogenesis of microri

123 onin and Aip1 promote cofilin-mediated actin filament disassembly, but the mechanism is somewhat cont

124 ogeneous distribution of mechanically linked filaments displayed by branched networks.

125 (2016) atomistic model of the thin filament displays a paucity of salt bridges and hydropho

126 We further demonstrate that RecA filaments disrupt G4 structures and remove G4 ligands in

127 we show that damage-induced Escherichia coli filaments divide asymmetrically, producing short daughte

128 he peak force, and these are confined to the filament domain containing myosin binding protein-C, the

129 Arp2/3-nucleated actin filaments drive crawling motility and phagocytosis in an

130 by promoting the degradation of intermediate filaments driving EMT, resulting in cell death.

131 rks, buffering the forces observed by myosin filaments during contraction.

132 the interdigitating thin (actin-containing) filaments during cyclical ATP-driven interactions toward

133 We quantify myosin II filament dwell times and processivity as functions of AT

134 A central open question is how collective filament dynamics emerges from interactions between indi

135 ndividuals with MSA are made of two types of filament, each of which consists of two different protof

136 This formin mediates slow filament elongation owing to a high probability of profi

137 ent biochemical activities of WAVE1 on actin filament elongation.

138 class-I TCP transcription factors in stamen filament elongation.

139 rate by modulating profilin occupancy at the filament end.

140 er actin filaments, increasing the number of filament ends available for polymerization or depolymeri

141 domains deliver profilin-actin complexes to filament ends.

142 to a high probability of profilin binding at filament ends.

143 ked' ground state where the FliJ coiled coil filament experiences angular fluctuations in an asymmetr

144 the complex structure of CPEB3 and an actin filament (F-actin).

145 Extrusion-based approaches including fused filament fabrication (FFF), jetting technologies includi

146 tion during filament bundle formation: actin filaments first align in the direction of the external f

147 y remain in an activated state near the thin filaments following relaxation, accounting for the delay

148 Keratin intermediate filaments form dynamic intracellular networks, which spa

149 DNA condensation protein that inhibits RAD51 filament formation and may antagonize other ssDNA-bindin

150 c cellular functions of NMII, such as myosin filament formation and nascent adhesion assembly, but no

151 ation in the BM without compromising bipolar filament formation but led to divergent adhesion phenoty

152 n of VPS28 helical interface residues blocks filament formation in vitro and autophagosome closure an

153 Filament formation transformed Orb2 from a translation r

154 ind to globular (G-)actin and regulate actin filament formation.

155 Filament-forming proteins in bacteria function in stabil

156 Here we present two novel filament-forming proteins in cyanobacteria.

157 DNA-bridging mode of H-NS proteins), whereas filament-forming proteins significantly increase the sti

158 Filament-free, bulk-RRAM cells instead store analogue st

159 c structure of the C. jejuni G508A flagellar filament from a 3.5- angstrom-resolution cryo-EM reconst

160 locate titin domains axially along the thick filament from its tip to the edge of the bare zone.

161 By comparing the structures and PTMs of tau filaments from CBD and Alzheimer's disease, it is found

162 Cryo-EM and mass spectrometry of tau filaments from CBD reveal that this conformer is heavily

163 In striated muscles, Tmods prevent actin filaments from overgrowing, whereas in non-muscle cells,

164 ansfer, metabolism, energy conservation, and filament growth in cable bacteria remains enigmatic.

165 mens, indicating that class-I TCPs stimulate filament growth.

166 process by considering the energy barrier a filament has to overcome for joining a bundle.

167 To our knowledge, similar filaments have not been observed in any other archaeal v

168 Intermediate filament (IF) cytoskeletal networks simultaneously suppo

169 The abundance and diversity of intermediate filaments (IFs) in the C. elegans intestine indicate imp

170 ogleins and desmocollins - link intermediate filaments (IFs) rather than actin to the plasma membrane

171 (i) thicker fibers of vimentin intermediate filaments, (ii) clusters of integrin alpha(5)beta(1), (i

172 slinks nonpolymerizing MT plus ends to actin filaments in axonal GCs, preventing MT depolymerization

173 suggest that conformational changes of actin filaments in cells could help to direct accessory bindin

174 in detail how consecutive depletion of thick filaments in individual sarcomeres within a myofibril af

175 successfully adopted to probe the 2-um-thick filaments in situ, being embedded inside the skeletal el

176 etal networks, but the roles of intermediate filaments in this process are poorly understood.

177 sure the mechanical response of single lamin filaments in three-dimensional meshwork.

178 ola PCC 7414 polymerized in vitro and formed filaments in vivo in several organisms.

179 ing and assembly of myosin into muscle thick filaments in vivo.

180 e cofilin/ADF family of proteins sever actin filaments, increasing the number of filament ends availa

181 Distinct sheath proteins localize to the filament inner and outer curvatures to define the superc

182 The filaments inside the bundle cannot be continuously actua

183 rminant CcfM (curvature-inducing coiled-coil filament interacting with the magnetoskeleton), which li

184 antagonistic forces establishes the range of filament interaction, which determines how the local fil

185 tion-induced alterations in tropomyosin-thin filament interactions underlie the altered regulatory ph

186 Tau aggregation into insoluble filaments is the defining pathological hallmark of tauop

187 Most of the enzyme filaments known to date have only been observed in cells

188 Here, we show that the nuclear lamina filament Lamin B2 (Lmnb2), whose expression decreases in

189 ters of contiguously bound cofilin along the filament lattice.

190 ged network geometry as well as reduction of filament length and number that was accompanied by abnor

191 ectural remodeling during cytokinesis at the filament level.

192 of endogenous LPL revealed that LPL adopts a filament-like distribution in vesicles.

193 siological conditions to form paired helical filament-like fibres in vitro in the absence of additive

194 ion of crvA, a gene encoding an intermediate filament-like protein necessary for curvature formation

195 l Tau isoform fibrils exhibit paired-helical-filament-like structures consisting of two protofibrils

196 ts suffering from Alzheimer's disease, while filaments made of the 3R-tau isoform, which contains onl

197 Filaments made up of different isoforms of tau protein a

198 Filaments made up of the 4R-tau isoform, which has four

199 Without MYO7B or actin filaments, many clathrin-coated pits fail to be severed

200 stacle is that cMyBP-C localization on thick filaments may be a key factor defining its interactions,

201 undles which do not contain tangled pairs of filaments, may appear surprising given that flagella are

202 We also packed filaments, membranes and macromolecules together, to obt

203 novel cytoplasmic giantin-based intermediate filament meshwork and in cytoplasmic bodies.

204 9-5.85 um, median 1.92 um) and contain actin filaments, microtubules, and cytokeratin 19-based interm

205 ." Myosin motors in domains further from the filament midpoint are likely to be activated and inactiv

206 ing A-bands become split and adjacent myosin filaments move in opposite directions while also sheddin

207 barbed ends and retain pointed ends of actin filaments near beads and we identified Spire's barbed-en

208 14-3-3 colocalizes with keratin filaments near cell-cell junctions in migrating mesendod

209 in (Arp)2/3 complex nucleates branched actin filament networks pivotal for cell migration, endocytosi

210 Here we show that branched actin filament networks, the main pushing machinery in cells,

211 anged by modification of the molecular actin filament nucleation and assembly rates.

212 observations on the kinetic pathway of actin filament nucleation and polymerization and possibilities

213 0-Arp2/3-mDia1 complex that greatly enhances filament nucleation.

214 The actin filament nucleator Arp2/3 complex is activated at cortic

215 tant and wild-type desmin generated chimeric filaments of seemingly normal morphology but with occasi

216 ch '4R' tauopathies from Pick's disease (the filaments of which are made of three-repeat (3R) tau iso

217 positions for tropomyosin cables along thin filaments on actin dominated by stereo-specific head-to-

218 e FtsZ, which was found to form treadmilling filaments on supported bilayers in vitro(1), as well as

219 Type IV pili are flexible filaments on the surface of bacteria, consisting of a he

220 nds on the local density of the cytoskeletal filaments on which motors operate.

221 e external force by initializing anisotropic filament orientations, then the chemical evolution of th

222 Endogenous lamin filaments portray non-Hookean behavior - they deform rev

223 In accordance with the force-from-filament principle, they may provide a connection to the

224 SF responsiveness relies on the "force-from-filament" principle involving extracellular tethers and

225 tion to stimulate robust and localized actin filament production in vivo.

226 In particular, lamin A/C, an intermediate filament protein critical for the interphase nuclear arc

227 GNIFICANCE STATEMENT Nestin, an intermediate filament protein highly expressed in neural progenitors,

228 d against the neuronal type III intermediate filament protein peripherin.

229 Nestin, an intermediate filament protein widely used as a marker of neural proge

230 by directly phosphorylating an intermediate filament protein, vimentin, thereby inhibiting assembly

231 trin regulates the placement of intermediate filament proteins forming a terminal web around the lume

232 In contrast, the roles of intermediate filament proteins in this process are poorly understood,

233 fluorescent labels attached to single actin filaments, provides precisions within tens of nanometers

234 e, myosin cross-bridges extending from thick filaments pull the interdigitating thin (actin-containin

235 A model based on polymerizing actin filaments pushing against mitochondria, thus generating

236 Upon addition of MMP-2, these filaments rapidly break into fragments prior to reassemb

237 ium and the linear nature of the effect, the filaments remain collimated throughout their paths.

238 molecular basis for its interaction with the filament, remains elusive.

239 opomyosin, likely disrupting the mutant thin filament response to calcium.

240 single-copy gene isolated from single hyphal filaments revealed nuclear heterogeneity both among and

241 defined sequence of intermediates, including filaments, rods, helices, and 2D rectangular plates, bef

242 We also find that the filament's pointed end is flatter than the remainder of

243 consistent with cooperativity between actin filament severing by myosin-induced forces and by gelsol

244 ent, consistent with a single cofilin having filament-severing activity.

245 t polymerizes into a single-stranded helical filament, shaping membranes into moderate-curvature tubu

246 multiscale modeling correlates cytoskeletal filament size with conformational changes inferred from

247 generic model for a nematic network in which filament sliding is driven by the action of motor protei

248 hat captures cortical MT plus ends to enable filament stabilization, as a host factor that enables HI

249 effects on actin, apparently increasing thin-filament stiffness and ultimately depressing contractile

250 providing a paradigm for connecting protein filament structure and mechanics to cellular organizatio

251 ophysical model for dynamic bundles of actin filaments submitted to an external load.

252 domains that associate with features of the filament, such as the 11 stripes of accessory proteins.

253 ponent CHMP4B and pUL51 forms ESCRT-III-like filaments, suggesting a direct role for pUL51 in promoti

254 nding and allow tropomyosin binding to actin filaments, suggesting that both proteins stabilize actin

255 genic amyloids, the hydrophilic core of Orb2 filaments suggests how some neuronal amyloids could be a

256 (2020) show that Lamin B2, a nuclear lamina filament supporting cardiomyocyte karyokinesis, also fac

257 ds of myosin interact with each other on the filament surface to form the interacting-heads motif (IH

258 troponin-tropomyosin complexes over the thin filament surface, which uncovers or blocks myosin bindin

259 Leaks are prevented by contractile actin filaments surrounding the diapedesis pore, keeping this

260 This extended network of neutral and ionized filaments surrounds the Large Magellanic Cloud (LMC) and

261 he precise role of the vimentin intermediate filament system in regulating the migration of amoeboid

262 ration entails networks and bundles of actin filaments termed lamellipodia and microspikes or filopod

263 isordered potential, focusing the light into filaments that display the features of branched flow: sc

264 assembly of two distinct bundles of helical filaments that have the same helical path but bind the m

265 n vitro, R406W-desmin formed unusually thick filaments that organized into complex filament aggregate

266 n emerin, and thereby controls nuclear actin filaments that spatially segregate viral DNA from inacti

267 RecA binds to ssDNA, forming filaments that stimulate proteolytic cleavage of the Lex

268 The actin cytoskeleton is a dynamic array of filaments that undergoes rapid remodeling to drive many

269 is determined by the electric charge of the filaments, the number of hydrophobic residues in the con

270 In the myosin-saturated state of the thin filament, there is a large additional shift in tropomyos

271 ding of RePRP reduces the abundance of actin filaments, thus diminishing noncellulosic polysaccharide

272 nsition from flat spiral polymers to helical filament to drive the formation of membrane protrusions,

273 contrast, because Wsp1 requires preexisting filaments to activate, it has been assumed to function e

274 n-muscle cells, Tmods bind actin-tropomyosin filaments to protect them from depolymerizing, not elong

275 ither talin nor vinculin alone recruit actin filaments to the membrane.

276 oskeletal components, microtubules and actin filaments, together with a microtubule motor, kinesin-1,

277 ious shapes, or adapt the orientation of the filaments towards the membrane during membrane remodelin

278 The filaments treadmill and show periods of rapid growth and

279 is required for CAP1 functions in both actin filament turnover and adhesion, and the novel mechanisti

280 fers the capability of interacting with both filament types onto melanophilin.

281 re parallel, longitudinal arrays by inducing filament unbundling within minutes.

282 y of interacting with microtubules and actin filaments unique to mammalian melanophilin or did it evo

283 k for incident development of HCM-LVSD (thin filament variants).

284 interaction, which determines how the local filament velocity depends on the polarity of the surroun

285 scribe a novel function for the intermediate filament vimentin in proteostasis as a spatial coordinat

286 A ultrastructure, such as membrane and filament, was approximated using multiple balls with fle

287 These filaments were identical between cases, but distinct fro

288 yses suggest that Bud3 stabilizes the single filaments, whereas Bud4 strengthens the interaction betw

289 In vitro, UNC-87 bundled actin filaments, whereas CLIK-1 bound to actin filaments witho

290 g of a torque-generating motor and a helical filament, which acts as a propeller.

291 Elastic energy stored in bent filaments, whose presence was confirmed by cryo-electron

292 RecA family of ATPases, which form a helical filament with single-stranded DNA (ssDNA) and ATP.

293 eletal protein titin that connects the thick filament with the sarcomere end, working as an I-band sp

294 spontaneously assembles into supramolecular filaments with a 100% CPT loading.

295 However, in vitro measurements on filaments with a sparse complement of heads detected a d

296 ient fabrication of such nanocellulose-based filaments with controlled and predictable properties.

297 Both mechanisms yield rapidly elongating filaments with mDia1 at their barbed ends and SPIN90-Arp

298 Cofilin binds actin filaments with positive cooperativity, forming clusters

299 s cerevisiae glucokinase, forms two-stranded filaments with ultrastructure that is distinct from that

300 tin filaments, whereas CLIK-1 bound to actin filaments without bundling them and antagonized UNC-87-m