ライフサイエンスコーパス: filopodial

1 Given that this filopodial aberration is similar to the phenotype observ

2 ce coiling and hence axial shortening of the filopodial actin bundle.

3 M10(Full)LZ moves on filopodial actin bundles of cells with a mean step size

4 lysis revealed that M10(Full)LZ meandered on filopodial actin bundles to both x- and y- directions.

5 G-actin diffusion by the porous structure of filopodial actin filament bundle, we used a particle-bas

6 owever, is limited by the connection between filopodial actin filaments and the membrane at the tip.

7 in family, which facilitates the assembly of filopodial actin filaments that are bundled by Fascin.

8 the rapid movement of myosin molecules along filopodial actin filaments.

9 rdination between elongation and bundling of filopodial actin filaments.

10 fluorescently tagged protein complexes along filopodial actin filaments.

11 Our previous work found that the filopodial actin polymerase VASP and consequently filopo

12 nism by which a cell can use rotation of the filopodial actin shaft to induce coiling and hence axial

13 omplex to maintain RhoA activity and promote filopodial actin-spike formation and invasive migration.

14 to promote lamellipodia formation and oppose filopodial actin-spike formation, and led to activation

15 in-containing 3 (FHOD3) pathway and generate filopodial actin-spike protrusions which drive invasion.

16 ssue asymmetry, but neglect the preferential filopodial activity along the convergent axis observed i

17 (W650A) or UAS-IR-EcR (core) showed moderate filopodial activity and normal, albeit reduced, adult-li

18 hr APF, when growth is characterized by high filopodial activity at both terminal and interstitial po

19 absent from filopodia, and thus its role in filopodial activity has remained unexplored.

20 Later in development, filopodial activity remains high but is confined to term

21 tenance of normal growth cone morphology and filopodial activity.

22 egrin alpha5beta1 interaction is involved in filopodial adhesion to the astrocytic matrix.

23 gration and PI3K/AKT signalling, but impairs filopodial alignment along AC processes, suggesting that

24 ogether with frictional coupling between the filopodial and cortical actin networks as the main retra

25 d morphogenesis and promote assembly of both filopodial and lamellipodial actin networks.

26 ons to probe their cellular environment with filopodial and lamellipodial activity.

27 on microscopy revealed that CXCL8-stimulated filopodial and microvilli-like protrusions that interact

28 e find that the growth cone is almost purely filopodial, and that it extends by a protrusive mode of

29 mplex dependent or independent, can initiate filopodial assembly by specific formins.

30 10 localizes in filopodia, and BMP-dependent filopodial assembly decreases when Myo10 expression is r

31 pose an extension of the existing models for filopodial assembly in which any cluster of actin filame

32 Surprisingly, filopodial assembly requirements differ between suspensi

33 ension cells, Arp2/3 complex is required for filopodial assembly through either formin.

34 ells only, mDia1 and VASP also contribute to filopodial assembly, and filopodia are disproportionatel

35 dicating that in addition to its function in filopodial assembly, Myo10 also participates in a requis

36 in, which enter the filopodial tube from the filopodial base and diffuse toward the filament barbed e

37 s eventually retract all the way down to the filopodial base and disappear.

38 motions to reach endocytic hot spots at the filopodial base.

39 e, whereas bleb initiation was biased toward filopodial bases, where the cytoskeleton exhibited local

40 ed that Diaphanous and Enabled each regulate filopodial behavior in vivo and defined a quantitative "

41 ls in filopodia that directly correlate with filopodial behavior.

42 and substrate adhesion gives rise to various filopodial behaviors.

43 MIIB-KD lowers migration speed and increases filopodial branching.

44 infected cells, either as membrane sheets or filopodial bridges, are present and may be involved in H

45 ganization from the lamellipodial network to filopodial bundle during bridge formation occurs in a pr

46 r order structures, such as lamellar meshes, filopodial bundles, and stress fibers.

47 actin cross-linker, providing stiffness for filopodial bundles, and that its dynamic behavior allows

48 These data demonstrate that filopodial Ca(2+) signals regulate axon outgrowth and gu

49 We show that filopodial cAMP transients regulate spinal axon guidance

50 n assembles into parallel bundles, and known filopodial components localize to the tip and shaft.

51 rporating Delta-Notch signaling by transient filopodial contacts between nonneighboring cells.

52 naptic terminals, and more putative efferent filopodial contacts onto inhibitory neurons.

53 odia, selects the fascin-actin bundle at the filopodial core for motility.

54 reduced Myo3A tip localization was decreased filopodial density along the cell periphery, identifying

55 e, Src2 or cortactin up-regulation increased filopodial density, length, and protrusion time, whereas

56 el prevented the dioxin-induced reduction in filopodial density.

57 to glutamate) was associated with increased filopodial density.

58 n-alpha leads to a loss of this suppression, filopodial destabilization, and reduced synapse formatio

59 nown filopodial markers (MyoX/Cdc42) and the filopodial disrupter, low-dose cytochalasin-B, we demons

60 dvance of the actin, and thus of the dynamic filopodial domain of the growth cone, while maintaining

61 xon drives the forward motion of the dynamic filopodial domain that defines the growth cone.

62 e, we developed a new 4D analysis method for filopodial dynamics and a data-driven computational mode

63 sin-10 function and a subsequent increase in filopodial dynamics and cell migration.

64 N-Cadherin controls both fast filopodial dynamics and growth cone stabilization.

65 f filopodia, and Src activity regulates both filopodial dynamics and local PY signaling.

66 te our technique in living cells, we measure filopodial dynamics and quantify spatiotemporal localiza

67 how it controls the spatial localization of filopodial dynamics and thus axon extension.

68 Filopodial dynamics are thought to control growth cone g

69 rvations suggest that neurotrophins regulate filopodial dynamics by depressing the activation of RhoA

70 Thus, intracellular control of filopodial dynamics converged on PI3K activation and PIP

71 skeletal cross-talk necessary for regulating filopodial dynamics during dorsal closure.

72 the intermittent signaling induced by these filopodial dynamics generates a type of structured noise

73 Farp1 regulates dendritic filopodial dynamics in immature neurons, indicating role

74 Analysis of motor axon filopodial dynamics in live embryos revealed that mutant

75 g spinning disk confocal microscopy to image filopodial dynamics in mouse resident Lifeact-EGFP macro

76 We propose that Ena/VASP proteins control filopodial dynamics in neurons by remodeling the actin n

77 In vivo imaging reveals that the dendritic filopodial dynamics of motoneurons map onto their recrui

78 However, filopodial dynamics were affected independently of polar

79 s failed to undergo the 52 hpf transition in filopodial dynamics, leading to axon stalling.

80 of ADF/cofilin mimics the effects of BDNF on filopodial dynamics, whereas ADF/cofilin inactivity bloc

81 ength, altered filopodial shape, and reduced filopodial dynamics.

82 ilopodia and that mutant HTT interferes with filopodial dynamics.

83 rtners with Dyn1, Mena, and Eps8 to regulate filopodial dynamics.

84 ved in transducing BDNF-induced increases of filopodial dynamics.

85 e, p75NTR regulates RhoA activity to mediate filopodial dynamics.

86 5NTR is necessary and sufficient to regulate filopodial dynamics.

87 in cytoskeletal regulation directs dendritic filopodial emergence or their subsequent maturation into

88 ytic fusion plays a relatively minor role in filopodial expansion.

89 ing, growth cones transition from stochastic filopodial exploration to the formation of a limited num

90 lso identify a transient period of MF bouton filopodial exploration, followed by refinement of sites

91 how in epithelial cells that the dynamics of filopodial extension and retraction are determined by th

92 the CC are highly dynamic, undergoing rapid filopodial extension and retraction.

93 cell adhesion and membrane blebbing precede filopodial extension and the onset of migration.

94 leading front of actin polymerization at the filopodial extension and thus could potentially enhance

95 e would be a highly efficient way to control filopodial extension dynamics.

96 Thus, filopodial extension is regulated by at least two indepe

97 Thus, Slit locally stimulates directional filopodial extension, a process that is required for sub

98 9 small-interfering RNA resulted in enhanced filopodial extension, decreased cell adhesion, increased

99 CTGF by specific antibody affected vascular filopodial extension, growth of the superficial vascular

100 ngiogenic effects by stimulating directional filopodial extension, whereas matrix metalloproteinase i

101 of filopodia-associated proteins during the filopodial extension-retraction cycle in a variety of ce

102 posure leads to a reduction in the number of filopodial extensions at the medial epithelial edge of t

103 Within the synapse, filopodial extensions emanating from CD4+ T cells make c

104 gnaling through Arp2/3 and Diaph2, decreased filopodial extensions on dendritic cells, and inhibited

105 formation of neurites and lamellipodial and filopodial extensions similar to those induced by activa

106 Cytonemes are actin-based filopodial extensions that have been found to orient tow

107 ntiful microtubules and the presence of fine filopodial extensions that penetrated the ending.

108 estrogen can also stimulate the formation of filopodial extensions, an early step in the formation of

109 ing cell shape changes, cell rearrangements, filopodial extensions, and convergent extension movement

110 s displayed giant mossy fiber terminals with filopodial extensions, demonstrating that not all mossy

111 co-localize in specific micro-domains within filopodial extensions, far from the cell body.

112 located around the cell periphery and within filopodial extensions.

113 changes in cell shape and the elaboration of filopodial extensions.

114 d along actin filaments in lamellipodial and filopodial extrusions.

115 addition, MAYP colocalized with cortical and filopodial F-actin in vivo.

116 ments, we demonstrate that lamellipodial and filopodial filament breaking contribute equally to the r

117 evealed that fascin rapidly dissociates from filopodial filaments with a kinetic off-rate of 0.12 s(-

118 ein, fascin, undergoes rapid cycling between filopodial filaments.

119 lar cell type expresses the lamellipodial or filopodial form of the actin machinery is essential to u

120 ordingly, Ena/VASP function was required for filopodial formation from the growth cone in response to

121 We also report that EIIIA promotes filopodial formation in alpha9beta1-expressing cells acc

122 lated genes 3 and 5 (PRG3 and PRG5) increase filopodial formation in various cell lines, independentl

123 , the mechanism by which fascin functions in filopodial formation is not clear.

124 Cdc42 induces filopodial formation through IRSp53, an Inverse-Bin-Amph

125 Dyn1 actin binding domain mutant inhibits filopodial formation, suggesting a role in actin elongat

126 , we demonstrate that it specifically blocks filopodial formation, tumour cell migration and invasion

127 Dyn1 knockdown reduces filopodial formation, which can be rescued by overexpres

128 ransfer analysis, and we explore its role in filopodial formation.

129 vented sprout extension but had no impact on filopodial formation.

130 he molecular mechanism of fascin function in filopodial formation.

131 es impair the cellular function of fascin in filopodial formation.

132 bundling actin, which is required for proper filopodial formation.

133 ne localization of Myosin-X, an initiator of filopodial formation.

134 or neurons, regulating calcium signaling and filopodial formation.

135 Ang II induced Cdc42 activation and filopodial formation.

136 toward BMP6 gradients via the regulation of filopodial function and amplification of BMP signals.

137 When we simulated filopodial growth in the presence of capping proteins, q

138 We also discovered that filopodial growth is strongly diminished upon increasing

139 ent with experiments, in terms of predicting filopodial growth retraction cycles and the average filo

140 ty by suppressing Abl signaling to stimulate filopodial growth while presumably reducing substratum a

141 mber of filaments are needed to generate net filopodial growth.

142 urons with amphetamine increased mobility of filopodial HA-DAT and accelerated HA-DAT endocytosis in

143 33 act downstream of the Rac-like GTPases in filopodial inhibition.

144 nce cytoskeletal function during growth cone filopodial inhibition.

145 e performed a kinetic-structural analysis of filopodial initiation in B16F1 melanoma cells.

146 ng and FH3 domains in cortical localization, filopodial initiation, and lengthening.

147 between cells and investigate how cell-level filopodial interactions drive tissue-level CE.

148 ne filopodia, coincident with an increase in filopodial L1 and beta-integrin.

149 Our results indicate that BDNF regulates filopodial length and number through a Rho kinase-depend

150 ed filaments, approximately 30, at which the filopodial length can reach a few microns.

151 n binding and unbinding leads to macroscopic filopodial length fluctuations, compared with minuscule

152 and relative protein concentration along the filopodial length for a broad range of signal distributi

153 The filopodial length increases as the membrane fluctuations

154 eals that one of the key limiting factors of filopodial length is diffusional transport of G-actin mo

155 vercome the membrane resistance and that the filopodial length is limited by buckling for 10-30 filam

156 CP depletion decreased filopodial length, altered filopodial shape, and reduced

157 osin-10 (Myo10) and its expression increases filopodial length, filopodial number, and Myo10-dependen

158 though inhibition of myosin II also enhances filopodial length, our results indicate that BDNF signal

159 del generates testable predictions about how filopodial length, rate of growth, and interfilopodial d

160 hoA blocks neurotrophin-induced increases in filopodial length, whereas inhibition of RhoA enhances f

161 led to approximately threefold increases in filopodial length, with the transport being mainly limit

162 e two pathways result in additive effects on filopodial length.

163 mediating neurotrophin-induced increases in filopodial length.

164 related with lamellipodial area but not with filopodial length.

165 sitive to neurotrophins but display enhanced filopodial lengths comparable with neurotrophin-treated

166 e counterparts, consistent with the enhanced filopodial lengths observed on mutant growth cones.

167 length, whereas inhibition of RhoA enhances filopodial lengths, similar to neurotrophin treatment.

168 Measurements of filopodial lifespan and length revealed that most filopo

169 ial growth retraction cycles and the average filopodial lifetimes.

170 ampal neurons with a concomitant increase in filopodial-like outgrowths, suggesting an effect on syna

171 on of mature dendritic spines to an immature filopodial-like phenotype in primary hippocampal culture

172 te migration off vessel, with rapid pericyte filopodial-like process formation between adjacent vesse

173 tinotectal synapses are formed on developing filopodial-like processes to a circuit in which RGC axon

174 ptic reticulum volume, with the emergence of filopodial-like protrusions from synaptic boutons of the

175 Myosin X filopodial localization is perturbed in fascin-depleted

176 sponds to a transition from lamellipodial to filopodial localization.

177 rich cellular protrusions containing VASP, a filopodial marker.

178 Using known filopodial markers (MyoX/Cdc42) and the filopodial disru

179 Filopodial matching also occurs during repair of laser w

180 The biological significance of our filopodial model and avenues for future development are

181 and that Ena/VASP regulates mDia2-initiated filopodial morphology, dynamics, and function.

182 of the growth cone generates an asymmetry in filopodial motility and PY signaling that promotes repul

183 ough the signaling cues underlying dendritic filopodial motility are mostly unknown, brain-derived ne

184 Positive guidance cues stimulate filopodial motility by locally increasing tyrosine phosp

185 e imaging of radial glial cells and measured filopodial motility in the intact albino Xenopus laevis

186 dritic protrusions and accelerates dendritic filopodial motility through an Abl kinase-dependent path

187 ndritic growth cones and filopodia, mediates filopodial motility, and does so via the phosphoinositid

188 ventional myosin with important functions in filopodial motility, cell migration, and cell adhesion.

189 To study filopodial motility, we collected and analyzed image dat

190 and Nf1 (neurofibromatosis type 1), enhanced filopodial motility.

191 the tips of extending filopodia and controls filopodial motility.

192 rmed a significant reduction in radial glial filopodial motility.

193 es PKG1 activation in glial cells to control filopodial motility.

194 Our results suggest that filopodial movement is not random, but responsive to syn

195 re, an inhibitor of Dyn GTPase, also reduced filopodial number and increased their lifetime.

196 Ena modulates filopodial number and length, thus influencing the speed

197 its expression increases filopodial length, filopodial number, and Myo10-dependent cell motility in

198 Silencing of Daam1 led to severe defects in filopodial number, integrity, and architecture, similar

199 Loss of TrpML leads to increased tracheal filopodial numbers, growth, and increased CNS ROS.

200 model for selection of lamellipodial versus filopodial organization in which CP is a negative regula

201 -RYK acts as a navigation system to instruct filopodial pathfinding, a process that is crucial for co

202 ve (post-UVR) conditions, which we call the "filopodial-phagocytosis model." This model also provides

203 Moreover, filopodial phospho-ERM levels are increased by incorpora

204 by increasing the formation of cytoskeletal filopodial precursors (patches) through localized microd

205 llary sprout tips (P < 0.02) and endothelial filopodial processes (P = 0.001).

206 Motile dendritic filopodial processes are thought to be precursors of spi

207 ogenous ephrinAs (EphAs) induce outgrowth of filopodial processes from astrocytes within minutes in r

208 cells continuously change shape and project filopodial processes in their direction of motion, sugge

209 Notably, MsEphrin could be detected on the filopodial processes of the EP cells that extended up to

210 horylation, decreased motility or eliminated filopodial processes respectively.

211 eporting in Nature, Sanders et al. implicate filopodial projections in Sonic hedgehog (Shh) patternin

212 within cells confounds attempts to identify filopodial proteins.

213 length and then contract over time; and that filopodial protrusion and expansion were affected by PAR

214 CC receptor signaling stimulates growth cone filopodial protrusion and that repulsive UNC-40-UNC-5 he

215 owth cones and for inhibition of growth cone filopodial protrusion caused by activated MYR::UNC-40 an

216 repulsive UNC-40-UNC-5 heterodimers inhibit filopodial protrusion in C. elegans.

217 s were required for the normal limitation of filopodial protrusion in developing growth cones and for

218 es required for UNC-6-mediated inhibition of filopodial protrusion involved in axon repulsion.

219 amily GTPase, Rif, as a potent stimulator of filopodial protrusion through a mechanism that does not

220 pendent processes such as membrane ruffling, filopodial protrusion, and cell motility.

221 ughter cells extend a long, basally oriented filopodial protrusion, building a de novo path along whi

222 h a rigid barrier, mimicking the geometry of filopodial protrusion.

223 mulation and inhibition of growth cone (e.g. filopodial) protrusion.

224 DOCK4 signalling is necessary for lateral filopodial protrusions and tubule remodelling prior to l

225 HS was present on filopodial protrusions appearing as a meshwork on the ce

226 g that the Xena/XVASP family of proteins and filopodial protrusions are non-essential for pathfinding

227 Lamellipodial and filopodial protrusions from the growth cone underlie mot

228 ascade for the formation of endothelial cell filopodial protrusions necessary for tubule remodelling,

229 mulated a marked induction of CK2-containing filopodial protrusions possessing budding viral particle

230 eveloping optic tectum extend highly dynamic filopodial protrusions within the tectal neuropil, the m

231 fascin are required for the organization of filopodial protrusions, Rac-dependent migration, and tum

232 growing actin filaments in lamellipodial and filopodial protrusions, thus corresponding to the tips o

233 uires motile, invasive behaviour and extends filopodial protrusions.

234 emporal protein concentration along flexible filopodial protrusions.

235 ave disorganized F-actin and display reduced filopodial protrusive activity at their leading edge.

236 ve turning, suggesting that local changes in filopodial PY levels may underlie growth cone pathfindin

237 We also explored filopodial regulation in cultured Drosophila cells and e

238 ligases are required for netrin-1-dependent filopodial responses, axon turning and branching, and fi

239 halasin D to disrupt F-actin assembly led to filopodial retraction and growth cone collapse and resul

240 ese microdomain transients promotes tracheal filopodial retraction and in turn modulate CNS gas excha

241 Using optical tweezers, we show that filopodial retraction occurs at a constant speed against

242 Netrin-1 causes endothelial filopodial retraction, but only when UNC5B is present.

243 The force exerted by filopodial retraction, however, is limited by the connec

244 y correlated with the initiation of tracheal filopodial retraction.

245 whereas activating neuronal MsEphrin led to filopodial retraction.

246 a coli) particles by (i) capturing along the filopodial shaft and surfing toward the cell body, the m

247 binding kinetics between integrins along the filopodial shaft and the ligands on the surrounding ECM

248 s known about how the actin filaments in the filopodial shaft are spatially organized to form a bundl

249 bundling protein and localizes all along the filopodial shaft, which differs from other formins that

250 to a striking loss of Daam1 localization to filopodial shafts, but not tips.

251 d centripetal flow, drove a lamellipodial-to-filopodial shape change in suspended cells, and induced

252 everal methods exist that analyze changes in filopodial shape, a software solution to reliably correl

253 pletion decreased filopodial length, altered filopodial shape, and reduced filopodial dynamics.

254 context at least, the analysis supports the filopodial signaling hypothesis.

255 fibronectin-rich 3D ECM, driven by RhoA and filopodial spike-based protrusions, not lamellipodia.

256 ment, both beta-actin immunofluorescence and filopodial spines were increased (from 70.57 +/- 1.09% t

257 odial actin polymerase VASP and consequently filopodial stability are negatively regulated via nondeg

258 s the barbed-end polymerase VASP to modulate filopodial stability during netrin-dependent axon guidan

259 M9-mediated ubiquitination of VASP creates a filopodial stability gradient during axon turning.

260 ases is a critical element of VASP dynamics, filopodial stability, and axon guidance.

261 ed, which promotes VASP tip localization and filopodial stability.

262 tip localization, VASP dynamics at tips, and filopodial stability.

263 conditions occurred through an intermediate filopodial stage, whereas bleb initiation was biased tow

264 n the CVP of bcar1-/- embryos produced fewer filopodial structures and did not detach efficiently fro

265 ing cell biological analysis of the delicate filopodial structures.

266 in to interact with and facilitate dendritic filopodial targeting of FGF22, triggering presynaptic ma

267 Since SDC2 also enhances filopodial targeting of NMDAR via interaction with the C

268 To examine the mechanisms of DAT filopodial targeting, we used quantitative live-cell flu

269 se a cell-based CE model based on asymmetric filopodial tension forces between cells and investigate

270 Filopodial-tension CE is robust to relatively high level

271 uits the Ena/WASP family protein Mena to the filopodial tip and protects elongating actin filaments f

272 Arp2/3 complex activators, self-assembly of filopodial tip complexes on the membrane, and outgrowth

273 In cultured cells, we observed that the filopodial tip localization of Myo3A lacking the kinase

274 the kinetic properties and the effect on the filopodial tip localization of the recombinant mouse myo

275 mediated ubiquitination of VASP reduces VASP filopodial tip localization, VASP dynamics at tips, and

276 Adhesion of a bead to the filopodial tip locally reduces actin polymerization and

277 The polymerization reactions at the filopodial tip require transport of G-actin, which enter

278 el by which myosin 10 rapidly targets to the filopodial tip via a sequential reduction in dimensional

279 were found to retract beads attached to the filopodial tip, and retraction was found to correlate wi

280 ticles of GFP-Myo5a can also move toward the filopodial tip, but at a slower characteristic velocity

281 e in a rapid and directed fashion toward the filopodial tip.

282 gineered to localize high levels of CDHR2 at filopodial tips acquired interfilopodial adhesion and ex

283 The resulting molecular traffic jams at filopodial tips amplify fluorescence intensities and all

284 quired for the delivery of fascin to growing filopodial tips at sufficient rates.

285 cking the motor region failed to localize to filopodial tips but still bound transiently at the plasm

286 llel actin filaments, which are elongated at filopodial tips by formins and Ena/VASP proteins.

287 in capping protein, is seen most strongly at filopodial tips during disassembly.

288 merization module became translocated to the filopodial tips in the presence of cargo complex, i.e.,

289 Indeed, the translocation to the filopodial tips was hampered by the diminished motor fun

290 yosin that transports the specific cargos to filopodial tips, and is associated with the mechanism un

291 ntact dynamics, phosphotyrosine signaling at filopodial tips, and lamellipodial protrusion.

292 r-forming module does not translocate to the filopodial tips.

293 a, whereas virtually no exchange occurred at filopodial tips.

294 oad-dependent processivity of myosin-10 as a filopodial transport motor.

295 hich leads to smaller G-actin flux along the filopodial tube compared with the prediction using the d

296 equire transport of G-actin, which enter the filopodial tube from the filopodial base and diffuse tow

297 During dendritic growth, both filopodial types undergo a process of stage-specific mor

298 nctions through development, or do different filopodial types with distinct functions exist?

299 tors, which may have differential effects on filopodial versus lammelipodial actin-based protrusions.

300 Our model provides a filopodial "winner-takes-all" mechanism that ensures the