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1 to explain the efficient bioluminescence of fireflies.
2 st predators as well as specialist predatory fireflies.
3 f biological oscillators such as neurons and fireflies.
5 lication of both viruses, generated suitable firefly and Renilla luciferase reporter constructs, resp
7 t-regulated shRNA transgenic lines targeting Firefly and Renilla luciferases, Oct4 and tumor suppress
8 PDAC cells genetically engineered to express firefly- and Gaussia luciferase, simplifying the ability
9 Many key advances in our understanding of firefly biology and signaling have been made over the pa
11 the physics underlying the varied colors of firefly bioluminescence remains elusive because it is di
12 l-1,2-dioxetanone, a more adequate model for firefly bioluminescence, and found a singlet quantum yie
13 ial for only one of the partial reactions of firefly bioluminescence, supporting the proposal that th
16 animal model to determine the ability of the FireFly camera system to detect fluorescent SLNs after a
17 s, were dissected with the assistance of the FireFly camera system, a fluorescence-capable endoscopic
20 bservation of selective DYRK1A inhibition by firefly d-luciferin, we have explored static and dynamic
23 ond time-resolved spectroscopic study of the firefly emitter oxyluciferin and two of its chemically m
24 hin the family Lampyridae, new insights into firefly flash control, and the discovery of firefly nupt
25 This review provides new insight into how firefly flash signals have been shaped by the dual evolu
26 excited-state keto-enol tautomerization of a firefly fluorophore, and it could be important in resolv
30 knockin mouse were genetically modified with firefly luciferase (APL(luc)) to allow tracking by biolu
31 the bioluminescent fusion reporters beta-cat firefly luciferase (beta-cat-FLuc) and beta-cat click be
32 rine CT26 colorectal cancer cells expressing firefly luciferase (CT26-Luc), and the ACE-CD vector was
34 yc activity in living subjects using a split Firefly luciferase (FL) complementation strategy to dete
35 etween the three luciferases [mtfl, tfl, and firefly luciferase (fl)] both in cell culture and in liv
36 s (SkMb), and fibroblasts (Fibro) expressing firefly luciferase (Fluc) and green fluorescence protein
37 ter with orthogonal substrate specificity to firefly luciferase (FLuc) and its derivatives such as Ak
38 for in vivo delivery using saRNA coding for firefly luciferase (fLuc) as a reporter protein in vivo.
39 very frequently use reporter enzymes such as firefly luciferase (FLuc) as indicators of target activi
40 g of mice infected with parasites expressing firefly luciferase (FLUC) driven by the SAG2D promoter,
41 noninvasively, we developed a chimeric EGFR-firefly luciferase (FLuc) fusion reporter to directly mo
45 m cells (gADSCs) were transduced with either Firefly luciferase (Fluc) or Gaussia luciferase (Gluc) r
46 la luciferase (RLuc), beta-galactosidase and firefly luciferase (FLuc) ORFs linked in frame and separ
49 LK1 confers MAPK specificity by activating a firefly luciferase (FLuc) reporter gene when the Ets-lik
50 its activity to an off-target effect on the Firefly luciferase (FLuc) reporter used in the developme
51 mouse model that constitutively expresses a firefly luciferase (FLuc) split reporter complementation
53 or VEEV (subtype IC strain 3908) expressing firefly luciferase (fLUC) to simulate mosquito infection
54 nsgenic reporter mouse strain that expresses firefly luciferase (Fluc) under the regulatory control o
55 imaging reporter mice in which expression of firefly luciferase (FLuc) was placed under the control o
56 at by combining stabilised colour mutants of firefly luciferase (FLuc) with the luciferin (LH(2)) ana
57 to synthesize d-luciferin, the substrate for firefly luciferase (Fluc), along with a novel set of ele
59 r finding that d-luciferin, the substrate of firefly luciferase (fLuc), is a specific substrate of AB
60 tation biosensor based on optical imaging of Firefly luciferase (FLuc), to quantitatively image p53 s
63 ic promoter virus expressing either a large, firefly luciferase (fLuc; 1,650 nucleotides), or small,
64 stably express green fluorescent protein and firefly luciferase (GFP(+)/Luc(+)) were used for the tra
67 ct organisms, we engineered an IkappaB alpha-firefly luciferase (IkappaB alpha-FLuc) fusion reporter.
68 ismate synthase (ICS1) promoter was fused to firefly luciferase (luc) and a homozygous transgenic lin
70 second construct was generated in which the firefly luciferase (Luc) gene was inserted in place of H
71 the red fluorescent protein (mCherry) or the firefly luciferase (Luc) genes are inserted into the RSV
73 2 strain D39, we transcriptionally fused the firefly luciferase (luc) to competence-specific genes an
74 ter mouse model that predominantly expresses firefly luciferase (luc2p) in the paw epidermis--the reg
75 st created a mutant (mtfl) of a thermostable firefly luciferase (tfl) bearing the peroxisome localiza
76 simplex virus type I thymidine kinase [tk], firefly luciferase [fl], and Renilla luciferase [rl]) pl
78 elongation errors by monitoring the level of firefly luciferase activity from a mutant allele inactiv
80 en complementary non-functional fragments of firefly luciferase allows direct detection of IP(3) in p
81 izing two different split-protein reporters, firefly luciferase and beta-lactamase, while also testin
83 inhibitor of metalloproteinases 3 (TIMP3) or firefly luciferase and designated them rQT3 and rQLuc, r
84 An intravenous injection of AAV8 containing firefly luciferase and eGFP under transcriptional contro
86 background) that constitutively express both firefly luciferase and enhanced green fluorescence prote
87 ng double-fusion reporter gene consisting of firefly luciferase and enhanced green fluorescent protei
88 asts were lentivirally transduced to express firefly luciferase and green fluorescent protein (GFP).
89 ts were genetically modified to express both firefly luciferase and green fluorescent protein (mH9c2)
90 FVB mice with a beta-actin promoter driving firefly luciferase and green fluorescent protein double
91 by sectioning mouse hearts (n=40) expressing firefly luciferase and green fluorescent protein into sl
92 ell line transduced with a vector expressing firefly luciferase and green fluorescent protein were tr
95 sion (TF; monomeric red fluorescent protein, firefly luciferase and herpes simplex virus thymidine ki
96 lar bioenergetics and the signal obtained by firefly luciferase and human sodium-iodide symporter lab
99 ransduced with an ubiquitin promoter driving firefly luciferase and monomeric red fluorescence protei
100 ally brighter signals from deep tissues than firefly luciferase and other bioluminescent proteins.
102 attached to the C-terminal fragment of split-firefly luciferase and the coiled-coil Fos, which is spe
103 i.v. administration of Ad vectors expressing firefly luciferase and to retarget virus to hepatic colo
105 ect effect on the FLuc reporter, implicating firefly luciferase as a molecular target of PTC124.
107 introduction of green fluorescent protein or firefly luciferase as fusions with replicase proteins.
110 l BRET-FRET energy transfer process based on firefly luciferase bioluminescence can be employed to as
112 e molecules are hydrolyzed to substrates for firefly luciferase by the enzyme fatty acid amide hydrol
116 new crystal structures of the product-bound firefly luciferase combined with the previously determin
120 Analogues of dihydrofolate reductase and firefly luciferase containing glycosylated amino acids a
123 elii YM strain (PyLuc) that stably expresses firefly luciferase driven by a constitutive promoter.
124 the constitutive hsp70 promoter, as well as firefly luciferase driven by the schizont-specific lisp2
127 harge-coupled device camera, the analysis of firefly luciferase fragment complementation in transient
128 mbinations of nonoverlapping and overlapping firefly luciferase fragments that can be used for studyi
129 ic islets (200 per recipient) expressing the firefly luciferase from FVB/NJ strain (H-2q) mice were t
130 binant CII proteins more efficiently protect firefly luciferase from insolubilization during heating
133 novel transcriptionally coupled IkappaBalpha-firefly luciferase fusion reporter and characterized the
135 herpesvirus 68) was constructed to express a firefly luciferase gene driven by the viral M3 promoter
136 veloped a transgenic mouse model wherein the firefly luciferase gene expression was dependent on the
137 -based methodology for rapidly integrating a firefly luciferase gene in somatic cells under the contr
138 this end, the APP1 promoter was fused to the firefly luciferase gene in the C. neofor-mans GAL7:IPC1
139 transfected with a reporter comprised of the firefly luciferase gene interrupted by an abnormally spl
140 explants from transgenic mice containing the firefly luciferase gene luc controlled by the mPer1 prom
142 g the IFN-stimulated gene 56 promoter-driven firefly luciferase gene stably integrated in a TLR3-expr
143 nstructed a di-cistronic reporter in which a firefly luciferase gene was linked to a chloramphenicol
144 ation of an adenovirus vector containing the firefly luciferase gene was measured serially and noninv
145 ression of the coding region of the enhanced firefly luciferase gene were employed to identify region
147 c and Cfluc are the N and C fragments of the firefly luciferase gene, respectively): Nfluc (1-475)/Cf
150 ic region was cloned between the Renilla and firefly luciferase genes, which acted as reporters of OR
153 translation of a downstream cistron encoding Firefly luciferase in a dicistronic expression vector.
155 to achieve specific and robust expression of firefly luciferase in the prostate glands of transgenic
161 by using dual-luciferase reporters, in which firefly luciferase is used as the retrotransposition ind
162 and resolubilization of thermally denatured firefly luciferase occurred independently of NEF activit
163 n HIV-1-derived lentiviral vector expressing firefly luciferase permitting the use of bioluminescence
165 erization state of each mutant using a split firefly luciferase protein fragment-assisted complementa
166 vity, as well as the bio distribution of the firefly luciferase protein in a series of three heart tr
167 ansfected with nonoxidized mRNA encoding the firefly luciferase protein were cultured in the presence
168 Plasmodium falciparum strain expressing the firefly luciferase protein, we present a luminescence-ba
170 Hence, we constructed biotinylated fused firefly luciferase proteins, immobilized the proteins on
171 on proteins that could lead to split Renilla/firefly luciferase reporter complementation in the prese
172 oying parasites with an integrated copy of a firefly luciferase reporter gene and a secondary flow cy
173 gnificantly reduce the expressions of both a firefly luciferase reporter gene and an ectopic MLH1 gen
175 ULTR1;2 promoter (2.2 kb) was fused with the firefly luciferase reporter gene to quantitatively repor
177 iana mutants for deregulated expression of a firefly luciferase reporter gene under the control of th
181 lated region and regulated the expression of firefly luciferase reporter in a dose-dependent manner.
183 ng furans situated 2, 5 or 12 bps apart in a firefly luciferase reporter plasmid caused a decrease in
185 because this factor inhibits expression of a firefly luciferase reporter under the control of the BAG
186 bioluminescence imaging using a constitutive firefly luciferase reporter, while TGFbeta signaling in
194 estern Reserve vaccinia virus that expresses firefly luciferase to infect wild-type C57BL/6 and TLR3-
195 tein fragment complementation assay based on firefly luciferase to investigate dimerization of chemok
196 nal siRNAs targeting a different site on the firefly luciferase transcript or endogenously expressed
197 A piggyBac donor plasmid modified to encode firefly luciferase under control of schistosome gene pro
198 to contain like green fluorescent protein or firefly luciferase under control of the cytomegalovirus
201 fusion proteins consisting of a thermostable firefly luciferase variant that catalyze yellow-green (5
203 0(13) total viral particles of an Adenoviral firefly luciferase vector with a cytomegalovirus (CMV) p
204 As a proof-of-principle, photoregulation of firefly luciferase was achieved in live cells by caging
206 ured fibroblasts, in which the expression of firefly luciferase was driven by the promoter of the cir
210 nd carboxy-fragment (395-550 amino acids) of firefly luciferase were fused to amino-terminal of Apaf-
212 n (FVB/NJ-luc) that constitutively expressed firefly luciferase were transplanted to various implanta
213 the fusion of green fluorescent protein and firefly luciferase with either nonstructural protein 2 o
214 strategy to identify a novel split site for firefly luciferase with improved characteristics over pr
215 rter mouse line that pairs the expression of firefly luciferase with quantifiable expression of a hum
216 vity in HeLa cells of siRNA duplexes against firefly luciferase with substitutions in the guide stran
217 colons of mice administered an mRNA encoding firefly luciferase with ultrasound and the D-luciferin s
218 (DF; enhanced green fluorescent protein and firefly luciferase) or triple fusion (TF; monomeric red
219 ATP as substrate, pyruvate kinase (PK), and firefly luciferase) to generate ATP, with measurement of
221 hermoprotection against heat inactivation of firefly luciferase, and (iv) sequence homology analysis
223 ontaining monomeric red fluorescent protein, firefly luciferase, and herpes simplex virus thymidine k
224 nterfering RNA (siRNA) against a model gene, firefly luciferase, and PEI25 or PEI87 afforded a 77% an
225 sHSPs with three different model substrates, firefly luciferase, citrate synthase, and malate dehydro
226 hat D-luciferin, the endogenous substrate of firefly luciferase, is a specific substrate for ABCG2.
227 e-fusion (TF) reporter gene that consists of firefly luciferase, monomeric red fluorescence protein,
228 ple-fusion reporter, fluc-mrfp-ttk (encoding firefly luciferase, monomeric red fluorescent protein, a
229 ate-forming enzyme superfamily that includes firefly luciferase, nonribosomal peptide synthetases, an
230 on-ribosomal peptide synthetases (NRPS), and firefly luciferase, perform two half-reactions in a ping
231 h luminescence levels that are comparable to firefly luciferase, thus enabling autonomous bioluminesc
232 cules from the murine Hmox1 locus, including firefly luciferase, to allow long-term, non-invasive ima
233 nto the cytoplasm, measured by expression of firefly luciferase, was increased more than 10-fold by e
234 transgenic T. cruzi Y luc strain expressing firefly luciferase, we prioritized the biaryl and N-aryl
236 ord chemiluminescence (CL) from an optimized firefly luciferase-ATP bioluminescence reaction system,
241 th the growth dynamics in our experiments of firefly luciferase-expressing Hep3B tumor xenografts and
242 on henipavirus pathogenesis, we generated a firefly luciferase-expressing NiV and monitored virus re
243 selective accumulation of i.v. administered firefly luciferase-expressing T cells in intracerebral x
260 siRNA in skin was tested by co-delivering a firefly luciferase/mutant K6a bicistronic reporter const
261 ntiviral vectors encoding the reporter genes firefly-luciferase and murine interleukin-10 were admini
263 trate that both the engineered and wild-type firefly luciferases tolerate much greater steric bulk at
264 f the C-terminal domain and conserved in all firefly luciferases, are each essential for only one of
266 They share 19 to 21% sequence identity with firefly luciferases, which produce light using ATP and t
268 ng animals that employs in situ formation of firefly luciferin from two complementary caged precursor
269 Once released, HCBT and D-cysteine form firefly luciferin in situ, giving rise to a bioluminesce
273 selectively reacts with H(2)O(2) to release firefly luciferin, which triggers a bioluminescent respo
276 ascades, design of peroxide-based medicines, firefly luminescence, and reductive repair of DNA photod
282 he steady-state and time-resolved spectra of firefly oxyluciferin complexed with luciferase from the
283 pectroscopy to compare the absorption by the firefly oxyluciferin lumophore isolated in vacuo and com
291 he ATP-dependent luciferase derived from the firefly Photuris pennsylvanica that was optimized using
292 system to examine the expression profiles of firefly reporter mRNAs bearing alternative combinations
293 we developed a dual-luciferase (Renilla and Firefly) reporter system for high-throughput screening (
294 luciferases obtained from beetle, including fireflies, result in novel properties and offer opportun
297 tion between S1P1 and beta-arrestin2 via the firefly split luciferase fragment complementation system
299 lampyrids, including evidence from Photinus fireflies that females choose their mates on the basis o