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1 or the inhibition of biofilm formation by V. fischeri.
2 urring, non-bioluminescent strains of Vibrio fischeri.
3 ated to promote symbiotic colonization by V. fischeri.
4 rum sensing proteins LuxR and LuxI of Vibrio fischeri.
5 n OMV production and biofilm formation by V. fischeri.
6 a scolopes and the luminous bacterium Vibrio fischeri.
7 opes and the bioluminescent bacterium Vibrio fischeri.
8 a second anthranilate-activating NRPS in N. fischeri.
9 rahaemolyticus, Vibrio vulnificus and Vibrio fischeri.
10 milar to that triggered by infection with V. fischeri.
11 ted to RscS-mediated biofilm formation in V. fischeri.
12 ironment to regulate biofilm formation by V. fischeri.
13 e loci contribute to biofilm formation in V. fischeri.
14 omonas aeruginosa; the model symbiont Vibrio fischeri.
15 for two CsrA-regulating small RNAs in Vibrio fischeri.
16 clic di-GMP in the control of motility of V. fischeri.
17 quorum sensing signaling pathway from Vibrio fischeri.
18 inally studied in the marine symbiont Vibrio fischeri.
19 modulating other symbiotic functions, in V. fischeri.
20 promoting colonization of E. scolopes by V. fischeri.
21 ls relative to that achieved by wild-type V. fischeri.
22 factor similar to the LuxR protein of Vibrio fischeri.
23 high density by the marine bacterium Vibrio fischeri.
24 Euprymna scolopes, the symbiotic host of V. fischeri.
25 and characterized a glnD:mTn5Cm mutant of V. fischeri.
26 an elevated bacterial toxicity to Aliivibrio fischeri.
27 ortant insights into biofilm dispersal by V. fischeri.
28 tes the aphrodisiac-like activity of live V. fischeri.
29 on the acute toxicity of OSPW toward Vibrio fischeri.
30 CRP, may help control cAMP-CRP activity in V.fischeri.
31 ts specific, bioluminescent symbiont, Vibrio fischeri.
32 positively control bioluminescence in Vibrio fischeri.
36 d Eisenia fetida, the marine bacteria Vibrio fischeri, a suite of ten prokaryotic species, and a euka
38 ted intracellular citrate levels in a Vibrio fischeri aconitase mutant correlate with activation of t
39 ould establish symbiosis, suggesting that V. fischeri acquires nutrients related to this compound wit
40 We have investigated the impact of the V. fischeri acyl-HSL synthase AinS on both luminescence and
41 st that the two quorum-sensing systems in V. fischeri, ain and lux, sequentially induce the expressio
42 ful colonization of E. scolopes, i.e. the V. fischeri ainS mutant failed to persist in the squid ligh
45 his system include the genome sequence of V. fischeri, an expressed sequence tagged library for E. sc
48 s important for symbiotic colonization by V. fischeri and establishes a paradigm for evaluating two-c
50 f the symbiosis between the bacterium Vibrio fischeri and its squid host, which can be observed direc
52 and bioluminescent (luxCDABE from Aliivibrio fischeri and Photorhabdus luminescens, NanoLuc) reporter
53 utualism between the marine bacterium Vibrio fischeri and the Hawaiian bobtail squid, we characterize
55 athed flagellum in both the mutualist Vibrio fischeri and the pathogen Vibrio cholerae promotes relea
56 n the symbiosis between the bacterium Vibrio fischeri and the squid Euprymna scolopes, which proceeds
59 oserine lactone synthase gene luxI of Vibrio fischeri and three genes that resemble the acylhomoserin
60 uorum sensing systems such as LuxR of Vibrio fischeri and TraR of Agrobacterium tumefaciens, there is
61 otein necessary for biofilm maturation by V. fischeri and, based on the conservation of bmp, potentia
62 ction between the Lux and Syp pathways in V. fischeri, and furthers our understanding of how the Lux
63 philus influenzae, Proteus mirabilis, Vibrio fischeri, and Pasteurella multocida are all cleaved by R
64 ADase activity in culture supernatants of V. fischeri, and this mutant initiated the light organ symb
65 a scolopes forms a natural symbiosis with V. fischeri, and utilizes the symbiont-derived bioluminesce
67 l regulation of TMAO reductase operons in V. fischeri appears to differ from that in previously studi
68 NADPH-utilizing flavin reductase from Vibrio fischeri are quite similar to that of the wild-type FRP(
69 This study lays the foundation for using V. fischeri as a model system for studying TMAO reductases
72 mbiosis between Euprymna scolopes and Vibrio fischeri As the juvenile host matures, it develops compl
73 work suggests that the tissue with which V. fischeri associates not only can detect bioluminescence
80 factor CysB is shown to be necessary for V. fischeri both to grow on several sulfur sources in vitro
81 ecologically distinct bioluminescent Vibrio fischeri by colonization and growth within the light org
82 strate that SypE controls biofilms in Vibrio fischeri by regulating the activity of SypA, a STAS (sul
83 popolysaccharide (LPS) or free lipid A of V. fischeri can trigger morphological changes in the juveni
84 ggregation, suggest a two-step process of V. fischeri cell engagement: association with host cilia fo
85 ctly and in real time, approximately five V. fischeri cells aggregate along the mucociliary membranes
86 rrounding mucus, the environment in which V. fischeri cells aggregate before migration into the organ
87 h inocula demonstrated that environmental V. fischeri cells aggregate during a 3 h period in host-she
90 -Seq dataset representing host-associated V. fischeri cells from colonized juvenile E. scolopes, as w
91 glycerol/tryptone-based medium, wild-type V. fischeri cells initially excrete acetate but, in a metab
96 a galactose-utilization mutant (galK) of V. fischeri colonized juvenile squid to wild-type levels, i
98 by SypE is a critical mechanism by which V. fischeri controls biofilm development and symbiotic colo
99 thus reveals a novel group of genes that V. fischeri controls through a sigma54-dependent response r
102 y demonstrated that oxygen consumption by V. fischeri CydAB quinol oxidase is inhibited by NO treatme
103 imitation or (ii) a mutation that renders V. fischeri defective in the synthesis of a homolog of the
104 id Euprymna scolopes by the bacterium Vibrio fischeri depends on bacterial biofilm formation on the s
107 Here, we show that the repressor NagC of V. fischeri directly regulates several chitin- and N-acetyl
108 t cAMP-CRP is active and important in Vibrio fischeri during colonization of its host squid Euprymna
112 rio symbiosis, the bacterial symbiont Vibrio fischeri encounters host-derived NO, which has been hypo
115 s model, we found that bioluminescence in V. fischeri ES114 is modulated by glucose and stimulated by
116 ble for this phenomenon, the lipid A from V. fischeri ES114 LPS was isolated and characterized by mul
118 ture fluids from the marine bacterium Vibrio fischeri ES114 prevent the growth of other vibrio specie
119 conditions, aerobactin production allows V. fischeri ES114 to competitively exclude Vibrio harveyi,
120 t known relatives, flrA mutant strains of V. fischeri ES114 were completely abolished in swimming cap
121 vnA and HvnB are proteins secreted by Vibrio fischeri ES114, an extracellular light organ symbiont of
124 Building on the genome project of Vibrio fischeri ES114, we used a comparative approach to identi
125 Here we report the genome sequence of V. fischeri ES114, which enters into a mutualistic symbiosi
127 nescent bacteria inhibition test with Vibrio fischeri exhibited that the TPs irgarol sulfoxide and te
130 Magnesium-dependent induction of Vibrio fischeri flagellar (Mif) biogenesis depends upon two dig
132 (i) selection of the specific partner Vibrio fischeri from the bacterioplankton during symbiosis onse
135 osition in a 1 mM bulk solution above Vibrio fischeri (gamma-Protebacteria-Vibrionaceae) bacterial bi
137 -Mbp genome sequence represents the first V. fischeri genome from an S. robusta symbiont and the firs
140 edicted, in the presence of NO, wild-type V. fischeri grew more slowly on hemin than a hnoX deletion
143 ion of the lux operon (luxICDABEG) of Vibrio fischeri has been intensively studied as a model for quo
146 the luminescence (lux) genes of symbiotic V. fischeri have been shown to be highly induced within the
150 n colonizing the light organ of the model V. fischeri host, the Hawaiian bobtail squid Euprymna scolo
151 id not compromise symbiotic competence of V. fischeri; however, levels of colonization of an ainS lux
152 reduced the inhibition of luminescence to V. fischeri, i.e., were beneficial for the bacteria, with P
154 rmed with a truncated lux operon from Vibrio fischeri, in the presence of 1-100 nM exogenous acyl-hom
155 discovered that the marine bacterium Vibrio fischeri induces sexual reproduction in one of the close
156 s, this work reveals a mechanism by which V. fischeri inhibits cellulose-dependent biofilm formation
158 lay a role in the normal process by which V. fischeri initiates its colonization of the nascent light
165 these results show that the flagellum of V. fischeri is a complex structure consisting of multiple f
166 cence emitted by the marine bacterium Vibrio fischeri is a particularly striking result of individual
168 During the early stages of colonization, V. fischeri is exposed to host-derived nitric oxide (NO).
169 uxICDABEG) of the symbiotic bacterium Vibrio fischeri is regulated by the transcriptional activator L
170 Genetic arrangement of the flrA locus in V. fischeri is similar to motility master-regulator operons
176 fied TMAO reductase activity in symbiotic V. fischeri isolates associated with the light organs of ad
179 it is possible that these features of the V. fischeri lipid A may underlie the ability of E. scolopes
181 ild-type and waaL strains showed that the V. fischeri LPS has a single O-antigen repeat composed of y
184 , we identified three novel regulators of V. fischeri luminescence and seven regulators that altered
191 ein and a number of other homologs of Vibrio fischeri LuxR that function as receptors for N-acylhomos
192 Taken together, these data indicate that V. fischeri LuxS affects both luminescence regulation and c
193 this study, we investigated the impact of V. fischeri LuxS on luminescence and colonization competenc
194 e closely related sexual species Neosartorya fischeri, many of which may have roles in the pathogenic
197 new subgroup of PTase genes from Neosartorya fischeri, Microsporum canis, and Trichophyton tonsurans
199 ly identified several non-Lux proteins in V. fischeri MJ-100 whose expression was dependent on LuxR a
200 ablishing that there is a LuxR regulon in V. fischeri MJ-100 whose genes are coordinately expressed d
201 re fully characterize the LuxR regulon in V. fischeri MJ-100, real-time reverse transcription-PCR was
203 To understand environmental influences on V. fischeri motility, we investigated migration of this org
209 scence reactions using FRP(H) and the Vibrio fischeri NAD(P)H-utilizing flavin reductase G (FRG(F)) i
210 ganisms and toxicity end points using Vibrio fischeri (nonspecific), specific fish macrophage antimic
212 acetylaszonalenin synthetase of Neosartorya fischeri NRRL 181 activates anthranilate to anthranilyl-
213 These studies indicate that the unusual V. fischeri O-antigen sugars play a role in the early phase
214 ent with that activity, introduction into V. fischeri of medium-copy plasmids carrying these genes in
216 a scolopes and the luminous bacterium Vibrio fischeri offers the opportunity to decipher the hour-by-
219 lation and to contribute to the growth of V. fischeri on cystine, which is the oxidized form of cyste
222 to be differentially expressed among the V. fischeri populations occupying the various colonization
223 these results suggest the biogeography of V. fischeri populations within the squid light organ impact
227 lar Microbiology, Dunn et al. report that V. fischeri produces an NO-inducible and NO-resistant alter
228 e here that the flavohaemoglobin, Hmp, of V. fischeri protects against NO, both in culture and during
230 h a recently developed metabolic model of V. fischeri provides us with a clearer picture of the metab
234 enes, we examined the protein patterns of V. fischeri quorum-sensing mutants defective in luxI, ainS,
239 ymna scolopes by the marine bacterium Vibrio fischeri requires the symbiosis polysaccharide (syp) gen
240 ilm formation by the marine bacterium Vibrio fischeri requires the Syp polysaccharide, but the involv
241 whether the net release of PG monomers by V. fischeri resulted from lytic transglycosylase activity o
242 gions of a number of flagellar operons in V. fischeri revealed apparent sigma(54) recognition motifs,
245 omonas shigelloides, Vibrio cholerae, Vibrio fischeri, Shewanella putrefaciens, and Pseudomonas aerug
247 arly stage of symbiotic initiation in the V. fischeri-squid model symbiosis, and more broadly it adds
248 describe the draft genome sequence of Vibrio fischeri SR5, a squid symbiotic isolate from Sepiola rob
249 tivity associated with whole cells of Vibrio fischeri strain ES114 was identified as the product of a
250 ation of juvenile E. scolopes; however, a V. fischeri strain lacking TMAO reductase activity displays
253 pared and analyzed the ain locus from two V. fischeri strains and a Vibrio salmonicida strain to expl
256 promotes successful squid colonization by V. fischeri, supporting its potential role in initiation of
261 is specifically colonized by cells of Vibrio fischeri that are obtained from the ambient seawater.
262 606 bp open reading frame was cloned from V. fischeri that encoded a protein, which we named LitR, th
263 have cloned a gene, designated flrA, from V. fischeri that encodes a putative sigma(54)-dependent tra
264 o high density, we identified a mutant of V. fischeri that exhibited an apparent defect in symbiosis
266 na scolopes and its bacterial partner Vibrio fischeri, the bacteria induce dramatic morphogenesis of
269 derivatives (1-4) isolated from Neosartorya fischeri, three of which had significant antimicrobial a
271 e understanding of the matrix produced by V. fischeri to enhance cell-cell interactions and promote s
273 accharide locus, syp, is required for Vibrio fischeri to form a symbiotic association with the squid
276 host-like pH increased the resistance of V. fischeri to the cationic antimicrobial peptide polymixin
277 s and its luminous bacterial partner, Vibrio fischeri, to define the impact of colonization on transc
278 on mediated by the syp gene cluster helps V. fischeri transition from a dispersed planktonic lifestyl
279 his association, we searched a library of V. fischeri transposon insertion mutants for those that fai
282 a component of the host defense, and that V. fischeri uses a cytotoxin-like molecule to induce host d
286 -scale mutagenesis of a class of genes in V. fischeri using a genomic approach and emphasizes the imp
287 iofilm formation and host colonization by V. fischeri via its impact on transcription of the symbiosi
290 Complete removal of toxicity toward Vibrio fischeri was achieved after ozonation followed by 28-day
291 The acute toxicity of OSPW toward Vibrio fischeri was reduced after the solar/chlorine treatment.
292 genetic tractability of the bacterium Vibrio fischeri, we explore the regulation of aox expression an
293 sion of LitR, the global regulator in Vibrio fischeri, we have performed a series of genetic and phen
295 other regulators of biofilm formation in V. fischeri, we screened a transposon library for mutants d
296 de molecules released by the bacteria Vibrio fischeri when it rotates its flagella prompts its host,
297 have focused on the LuxR protein from Vibrio fischeri, which activates gene transcription in response
298 r the marine bioluminescent bacterium Vibrio fischeri, which exists either in a free-living state or
299 scolopes and its beneficial symbiont Vibrio fischeri, which form a highly specific binary mutualism.
300 f the bioluminescent marine bacterium Vibrio fischeri with its animal host, the Hawaiian bobtail squi