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1 ng like time-of-flight, gated detection, and fluorescence lifetime.
2 e speed of raster-scanned imaging imposed by fluorescence lifetime.
3 ics of their direct surroundings, with their fluorescence lifetime.
4 ned intracellular localization of MB and its fluorescence lifetime.
5 and GAF show blue-shifted emission with long fluorescence lifetimes.
6 rinsic fluorophores, based on their distinct fluorescence lifetimes.
7 ts ((13)C-AKIE) and decrease of 4-Cl-aniline fluorescence lifetimes.
8 ed substantial intermolecular variability in fluorescence lifetimes.
9 orescence quantum yields, Stokes shifts, and fluorescence lifetimes.
11 nt component (qE) and stable (1) chlorophyll fluorescence lifetime; (2) amplitude of the fluorescence
12 ts strong fluorescence ( F = 0.48) with long fluorescence lifetime (5.6 ns) and large Stokes' shift,
15 The instrument is capable of near real-time fluorescence lifetime acquisition in multiple spectral b
16 concentration information is encoded in the fluorescence lifetime across multiple spectral bands.
17 nced fluorescence emission, and an increased fluorescence lifetime, all indicating strong excitonic c
22 ication of the phasor approach to study NADH fluorescence lifetime and emission allowed us to identif
23 Independent classification analysis based on fluorescence lifetime and on Raman spectra discriminated
27 urement that captures information about both fluorescence lifetime and spatial position of the probes
29 e of plasma membrane tension by changing its fluorescence lifetime and thus allows tension imaging by
30 T spectroscopy that includes measurements of fluorescence lifetime and two- and three-color FRET effi
31 ree' approach, combining 2-photon excitation fluorescence lifetimes and emission spectral imaging wit
32 pic methods such as fluorescence anisotropy, fluorescence lifetimes and fluorescence quenching measur
34 ch is supported by an overall increased mean fluorescence lifetimes and significantly reduced water a
35 rption in a wide range of UV/vis, acceptable fluorescence lifetime, and effective intramolecular char
36 he combination of a high quantum yield, long fluorescence lifetime, and emission above 600 nm is poss
38 steady-state fluorescence, phosphorescence, fluorescence lifetime, and phosphorescence lifetime meas
39 tween phycobilisome components, (ii) shorter fluorescence lifetimes, and (iii) red shift in the emiss
40 emissive substitute for G with good QY, long fluorescence lifetimes, and exquisite sensitivity to loc
41 alize AuNPs with CdTe/CdS QDs to modulate QD fluorescence lifetimes, and nucleate the formation of fl
42 absorption and emission spectra, luminosity, fluorescence lifetimes, and two-photon absorptivity.
44 g a large emission cross-section and a short fluorescence lifetime as the gain medium, a stable LGS Q
48 brane leakage was separately measured by the fluorescence lifetime-based calcein leakage assay and th
50 e have designed a cell-permeable T-sensitive fluorescence lifetime-based nanoprobe based on lipophili
51 and mechanism of leakage is measured by the fluorescence lifetime-based vesicle leakage assay using
54 analysis of the correlation function of the fluorescence lifetime by improving the estimation of the
57 chlorophyll fluorescence quantum yields and fluorescence lifetimes clearly indicate that the presenc
59 e, we take advantage of previously published fluorescence lifetime correlation spectroscopy which rel
60 properties and to use them for single-color fluorescence lifetime cross-correlation spectroscopy (sc
66 n, multi-modal - two-photon fluorescence and fluorescence lifetime (FLIM) - microscopy and imaging fl
67 ingle-cell imaging methods for metabolism by fluorescence lifetime (FLIM) of NADH and signaling by ki
69 of ex vivo viable epidermis showed a stable fluorescence lifetime for unpatched areas of ~1000 ps up
70 myosins tested, we found two populations of fluorescence lifetimes for individual myosin molecules,
72 pmental, or "D-trajectory", that consists of fluorescence lifetime from different stages of mouse pre
74 As minocycline and tazarotene have distinct fluorescence lifetimes from the lifetime of the skin's a
75 ave a large fluorescence quantum yield, long fluorescence lifetime, good photostability, and an emiss
76 y limited class of fluorophores, with a long fluorescence lifetime (>10 ns) and fluorescence beyond 5
77 spectrometry, immunoelectron microscopy and fluorescence lifetime image microscopy based on Forster
79 nent analysis to analyze pairs of two-photon fluorescence lifetime images of stratum basale and strat
80 ual colour FLIM method we are able to detect fluorescence lifetime images of two donors to simultaneo
81 M and a viable route towards multi-megapixel fluorescence lifetime images, with a proof-of-principle
82 Here we present a technique using two-photon fluorescence lifetime imaging (2pFLIM) with new FRET bio
84 veloped an analytical strategy based on FRET-fluorescence lifetime imaging (FLIM) and fluorescence cr
86 to perturb NAD(P)H metabolism, we find that fluorescence lifetime imaging (FLIM) differentiates quan
89 mer to its micelle displays that time-domain fluorescence lifetime imaging (FLIM) is able to rapidly
90 ascular ultrasound (IVUS) with multispectral fluorescence lifetime imaging (FLIm) that enables label-
91 ion was characterized nondestructively using fluorescence lifetime imaging (FLIm) to identify regions
93 Using multiangle light scattering (MALS), fluorescence lifetime imaging (FLIM), and FRET analyses,
94 tilization, measured with two-photon NAD(P)H fluorescence lifetime imaging (FLIM), was matched in the
96 ctions in situ Here, we used high-resolution fluorescence lifetime imaging (FLIM)-FRET of HeLa cells
100 resonance energy transfer (FRET) measured by fluorescence lifetime imaging (FLIM-FRET) and identified
101 eveloped to perform quantitative macroscopic fluorescence lifetime imaging (MFLI) over a large field
102 advanced quantitative-time-resolved imaging (Fluorescence Lifetime Imaging and Fluorescence Correlati
103 fields of biomedical sensors, spectroscopy, fluorescence lifetime imaging and in the design of many
109 s been delineated through spatially resolved fluorescence lifetime imaging microscopy (FLIM) and fluo
111 -factors NADH and FAD with quantitation from Fluorescence Lifetime Imaging Microscopy (FLIM) as a mea
114 sment of processes such as fibrosis, whereas fluorescence lifetime imaging microscopy (FLIM) enables
117 phases could be imaged with high contrast by fluorescence lifetime imaging microscopy (FLIM) on giant
119 Subsequently, we evaluated the probe and fluorescence lifetime imaging microscopy (FLIM) techniqu
122 rescent molecular rotors in combination with Fluorescence Lifetime Imaging Microscopy (FLIM) to image
123 l two-photon microscopy and frequency-domain fluorescence lifetime imaging microscopy (FLIM) to map c
125 e use of pulsed interleaved excitation (PIE)-fluorescence lifetime imaging microscopy (FLIM) to measu
128 ssected into its components, and chlorophyll fluorescence lifetime imaging microscopy (FLIM) was used
129 t of microscopic viscosity in live cells via fluorescence lifetime imaging microscopy (FLIM) while al
130 time property could be visually mapped using fluorescence lifetime imaging microscopy (FLIM), allowin
132 ed the fluorescence decay of anthocyanins by fluorescence lifetime imaging microscopy (FLIM), in both
134 ET imaging advantages normally attributed to fluorescence lifetime imaging microscopy (FLIM), such as
135 or image-correlation spectroscopy of histone fluorescence lifetime imaging microscopy (FLIM)-Forster
137 ed a novel Forster resonance energy transfer-fluorescence lifetime imaging microscopy (FRET-FLIM)-bas
138 e, we used Forster resonance energy transfer/fluorescence lifetime imaging microscopy (FRET/FLIM) com
140 ng the first single-shot spectrally resolved fluorescence lifetime imaging microscopy (SR-FLIM).
144 rmore, our Forster resonance energy transfer-fluorescence lifetime imaging microscopy analysis indica
147 scopy techniques, including FRET detected by fluorescence lifetime imaging microscopy and single-cell
150 tracing, histone mass spectrometry, and NADH fluorescence lifetime imaging microscopy in these cells,
151 des an additional channel for multiparameter fluorescence lifetime imaging microscopy of green fluore
154 ing two- and three-color dSTORM supported by fluorescence lifetime imaging microscopy we identified h
156 , using fluorescence confocal microscopy and fluorescence lifetime imaging microscopy with the phasor
157 ologic evaluation, we apply the phasor-FLIM (Fluorescence Lifetime Imaging Microscopy) method to capt
159 rmed "molecular rotors", in combination with Fluorescence Lifetime Imaging Microscopy, for monitoring
160 raction with beta2 integrins, as revealed by fluorescence lifetime imaging microscopy, leading to int
162 vo by fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy, mediated throu
166 ransfer-based sensor for TrkB and two-photon fluorescence lifetime imaging microscopy, we monitor Trk
167 Along with cell cycle progression, utilizing fluorescence lifetime imaging microscopy-based Forster r
168 ing a fluorescence resonance energy transfer/fluorescence lifetime imaging microscopy-based Ras imagi
169 minescence-based mammalian interactome), and fluorescence lifetime imaging microscopy-fluorescence re
177 identification of submicroscopic domains by fluorescence lifetime imaging microscopy; 3), elucidatio
178 eep neural network (DNN) architecture, named fluorescence lifetime imaging network (FLI-Net) that is
179 y for minimizing the background influence in fluorescence lifetime imaging of live cells and sub-cell
180 sfunction and oxidative stress determined by fluorescence lifetime imaging of NADH and kidney fibrosi
183 this study was to investigate the benefit of fluorescence lifetime imaging ophthalmoscopy (FLIO) for
185 ore specialized microscopy techniques, e.g., fluorescence lifetime imaging or two-photon excited fluo
190 le and can be applied to many multicomponent fluorescence lifetime imaging targets that require cellu
191 its activity directly in single cells using fluorescence lifetime imaging to detect Forster resonanc
192 by fluorescence resonance energy transfer by fluorescence lifetime imaging to interact directly with
195 obtained by the phasor approach to confocal fluorescence lifetime imaging, a graphical method that d
197 , brightfield microscopy, histochemistry and fluorescence lifetime imaging, these autofluorescent par
198 les a diverse range of applications, such as fluorescence lifetime imaging, time-of-flight depth imag
201 ed via fluorescent ratiometric detection and fluorescence lifetime imaging; it was found that lysosom
203 ty in visualizing intracellular processes by fluorescence-lifetime imaging microscopy (FLIM) measurem
204 or can be used to image PI4KB in cells using fluorescence-lifetime imaging microscopy (FLIM) microsco
205 ally, fluorescence-resonance energy transfer-fluorescence-lifetime imaging microscopy experiments ind
206 arbons on the external surface, evidenced by fluorescence-lifetime imaging microscopy, are principall
207 findings are complemented experimentally by fluorescence-lifetime imaging microscopy/fluorescence re
208 study addressed this requirement by joining fluorescence-lifetime imaging microscopy/phasor multipho
209 n reaction,(13)C-nuclear magnetic resonance, fluorescence-lifetime imaging, mass spectrometry-based m
211 chondria of cancer cells, as well as shorter fluorescence lifetime in cancer relative to normal cells
215 GFP and EGFP can be clearly distinguished by fluorescence lifetime in various models, including mamma
218 s well suited to accurately quantify complex fluorescence lifetimes in cells and, in real time, in in
219 f 7-aminocoumarin dyes that have distinctive fluorescence lifetimes in different solvation environmen
222 o show that the origin of PIFE is the longer fluorescence lifetime induced by the local protein envir
224 ore the possibilities of using the available fluorescence lifetime information in PIE-FI experiments.
225 " embedded in the microbubble surface, whose fluorescence lifetime is directly related to the viscosi
227 tly labeled drugs, using fluorophores with a fluorescence lifetime larger than the rotational correla
228 ee Ag-In-S ternary quantum dots (t-QDs) with fluorescence lifetimes (LTs) of several hundred nanoseco
229 h high-performance liquid chromatography and fluorescence lifetime measured at 380-400 nm (R = -0.76,
233 is the demonstration of how high-throughput fluorescence lifetime measurements correlate well to cha
234 fluorescence anisotropy decay and picosecond fluorescence lifetime measurements for the flavin reveal
236 ic methods listed above and by time-resolved fluorescence lifetime measurements using a complementary
237 ques (UV, both steady state fluorescence and fluorescence lifetime measurements, circular dichroism (
242 phoresis, quantitative mass spectrometry and fluorescence lifetime microscopy to characterise a serie
243 vel microscopic technique, comparable to the fluorescence lifetime microscopy, enables its applicatio
244 -pull probes with the mechanosensitivity and fluorescence lifetime needed for practical use in biolog
245 ted single photon counting revealed that the fluorescence lifetime of (ts)T (tau = 4-11 ns) was short
247 er by biophysical studies: the excited-state fluorescence lifetime of a complex between ReAsH and a p
248 onitored by atomic force microscopy, and the fluorescence lifetime of Alexa-labeled Tau (time-correla
249 phagosomes become acidified and the average fluorescence lifetime of EGFP is known to be affected by
250 the simultaneously recorded images based on fluorescence lifetime of LHCII and fluorescence anisotro
251 uantifying FRET is to measure changes in the fluorescence lifetime of the donor fluorophore using FLI
255 room-temperature emission efficiency and the fluorescence lifetime of the restrained cyanine are not
260 in the absorption and emission spectra with fluorescence lifetimes of 1.3 ns, indicating the formati
263 demonstrate that mCherryTYG is an excellent fluorescence lifetime pH sensor that significantly expan
265 ty of responding to low pH by decreasing the fluorescence lifetime, present in the wild-type protein,
268 are combined to correlate the dependence of fluorescence lifetime reduction on the spectral overlap
269 narrow defined tunable emission peak, longer fluorescence lifetime, resistance to photobleaching and
271 1038 cm(-1)) bands in the Raman spectrum and fluorescence lifetime shortened by 0.4 ns compared to un
274 cP at 40 degrees C was completed within the fluorescence lifetime, so that the rotational time const
276 inding isotope effects (BIEs), time-resolved fluorescence lifetimes, Stokes shifts, and extended grap
279 s high as 0.93 in nonpolar solvents, and the fluorescence lifetimes (tau(F)) vary from 1.50 to 3.01 n
280 g average homo-FRET rates (k(FRET)), average fluorescence lifetimes (tau), and average anisotropies o
281 hin plant cells, the amplitude-weighted mean fluorescence lifetime (taum ) correlated with distinct s
282 how in response to doxorubicin, NAD(P)H mean fluorescence lifetime (taum) and enzyme-bound (a2%) frac
284 frared region (730 nm) is observed with long fluorescence lifetimes that range from 30 to 860 ns, dep
285 which exploits high sensitivity of the OGB-1 fluorescence lifetime to nanomolar Ca(2+) concentration
286 , a technique which utilizes a fluorophore's fluorescence lifetime to probe changes in its environmen
288 he CH3NH3SnI3 film effectively increases the fluorescence lifetime up to 10 times and gives diffusion
289 to-switchable nanogel that exhibits variable fluorescence lifetime upon photoisomerization-induced en
290 nditions are associated with altered NAD(P)H fluorescence lifetimes, use a simple cell model to confi
291 , followed by measurement and imaging of the fluorescence lifetime using multiphoton excitation.
293 presentation to analyze changes in Laurdan's fluorescence lifetime we obtain two different phasor tra
294 th a time constant >10 ns, comparable to the fluorescence lifetime, we used electron spin resonance s
295 progressive internalization of EGFP-E. coli, fluorescence lifetimes were acquired and compared to con
297 ncreases from 0.22 to 0.96) while long-lived fluorescence lifetimes were observed between 1.8-2.4 ns.