ライフサイエンスコーパス: fluorescent substrate

1 rojections as a serotonin transporter (SERT) fluorescent substrate.

2 ow one single enzyme when acting on a non-UV-fluorescent substrate.

3 al detection of GrB with an enzyme-activated fluorescent substrate.

4 luding ADAM12 for which there is no reported fluorescent substrate.

5 for CS activity based on incorporation of a fluorescent substrate.

6 were prepared and over-laid with a quenched fluorescent substrate.

7 ymatic activity as revealed using a quenched fluorescent substrate.

8 e of both a specific cellular activity and a fluorescent substrate.

9 tide nucleic acids, flipper force probes, or fluorescent substrates.

10 ed and synthesized a series of absorbent and fluorescent substrates.

11 hthaldehyde (2-NA) as cytochrome P450 (P450) fluorescent substrates.

12 utant showed the most effect on transport of fluorescent substrates.

13 esterase (S-depalmitoylation) activity using fluorescent substrates.

14 (HRP) enzyme is used for catalyzing the non-fluorescent substrate, 10-Acetyl-3,7-dihydroxyphenox-azi

15 his assay, we investigated substitution of a fluorescent substrate, 2'-(2-benzothiazol)-6'-hydroxyben

16 The fluorescent substrate 4-(4-(dimethylamino)phenyl)-1-meth

17 h could attenuate 35% of FAP cleavage of the fluorescent substrate Ala-Pro-7-amido-4-trifluoromethylc

18 efficiently quenched ( approximately 99.9%) fluorescent substrates also permit assessment of GCase i

19 of kinetic experiments and the behavior of a fluorescent substrate analog are consistent with nonidea

20 There is currently no evidence that the fluorescent substrate analog is turned over by the toxin

21 enzymes tightly bound trinitrophenyl-ATP, a fluorescent substrate analog, suggesting that there are

22 Displacement of the fluorescent substrate analogue methylanthraniloyl ADP (m

23 ues to other transferase enzymes that accept fluorescent substrate analogues.

24 ion of MRP1 both by monitoring the efflux of fluorescent substrate and by their ability to confer res

25 ssessed by a novel assay using a purchasable fluorescent substrate and thermal shift.

26 ssay of the SIRTs has been devised using the fluorescent substrate and these reagents.

27 degradation in cell extracts of model Q-rich fluorescent substrates and peptides containing 10-30 Q's

28 rate of N-phenyl-1-naphthylamine (MexAB-OprM fluorescent substrate) and extracellular exopolysacchari

29 rding uptake of endogenous substrates or the fluorescent substrate APP(+)(4-(4-dimethylamino)phenyl-1

30 However, methods based on monitoring fluorescent substrates are limited.

31 These results show that these fluorescent substrates are very valuable tools in FAAH a

32 iological substrate and a synthetic quenched fluorescent substrate as well as binding of the specific

33 s in the medium was assayed using a quenched fluorescent substrate, as well as with a collagen fibril

34 hibition of MMP-9 activity, verified using a fluorescent substrate assay, prevented the increase in p

35 n the medium was determined using a quenched fluorescent substrate assay, while specific collagenases

36 Catalase activities were measured with a fluorescent substrate assay.

37 tect ASM activity and diagnose NPD using the fluorescent substrate BODIPY C12-SPM and reverse-phase h

38 ically monitor the uptake of radiolabeled or fluorescent substrates but face limitations regarding th

39 were found to be similar using two quenched fluorescent substrates, but Delta249-451 collagenase-3 f

40 ld be identified in intact myocytes with the fluorescent substrate C12-fluorescein di-beta-galactopyr

41 By preloading target cells with the betaLa fluorescent substrate CCF2-AM, we obtained viral entry k

42 In vivo interstitial MMP activity (fluorescent substrate cleavage; 943+/-59 versus 1210+/-7

43 limited to the colorimetric G-quadruplex or fluorescent substrate cleaving NAzymes.

44 The use of a fluorescent substrate combined with CE separation has en

45 both rat and human mEH was obtained with the fluorescent substrate cyano(6-methoxy-naphthalen-2-yl)me

46 ity to aid the design of internally-quenched fluorescent substrates derived from bradykinin.

47 Also the fluorescent substrate, DEVD-AFC, is cleaved 2-4-fold mor

48 It hydrolyzes the fluorescent substrate Dns-PLALWAR at the Ala-Leu bond wi

49 nd imaging properties of a range of SNAP-tag fluorescent substrates, enabling the selection of optima

50 Fluorescent substrate excretion assays indicate that Bse

51 the cellular accumulation of acriflavine, a fluorescent substrate for a number of resistance-nodulat

52 omic fluorescence, DCF), we discovered a new fluorescent substrate for Akt, also known as protein kin

53 Here we report a red-shifted fluorescent substrate for ALDH, AldeRed 588-A, for label

54 ehydrogenase (ALDH) activity, we developed a fluorescent substrate for ALDH, termed BODIPY aminoaceta

55 A novel fluorescent substrate for beta-galactosidase (Fluor-X-Ga

56 Here we identify Nile red as a new fluorescent substrate for CaCdr1p, CaCdr2p, and CaMdr1p.

57 a-D-galactopyranoside (FDG), a commonly used fluorescent substrate for enzymological studies.

58 h (lambda(ex) = 600 nm, lambda(em) = 700 nm) fluorescent substrate for the chymotrypsin-like active s

59 cterial MTANs and use 2,6-diaminopurine as a fluorescent substrate for yeast adenine phosphoribosyltr

60 r identification and characterization of new fluorescent substrates for ABCB1, ABCC1, and ABCG2.

61 Fluorescent substrates for each of these active sites ha

62 clude that these two peptides can be used as fluorescent substrates for high-throughput screening for

63 ethylfluorescein diacetate (CMFD), which are fluorescent substrates for the bile acid and the nonbile

64 In this paper we describe novel fluorescent substrates for the human ADAM family members

65 nsport, which was followed by extrusion of a fluorescent substrate from the cells.

66 trate to product, CE is used to separate the fluorescent substrate (*GTP) from the fluorescent produc

67 Quenched fluorescent substrates have been used to analyze the rat

68 in and can compete with the transport of the fluorescent substrate Hoechst 33342.

69 icant loss of Ran-mediated nuclear uptake of fluorescent substrate in digitonin-permeabilized HeLa ce

70 it loses linearity as the absorbance of the fluorescent substrate increases with concentration.

71 sphatase, that catalyzes the hydrolysis of a fluorescent substrate leading it to fluoresce.

72 , thus allowing the selective release of the fluorescent substrates MB(+) or Th(+).

73 ke activity as assessed by the cleavage of a fluorescent substrate N-acetyl-Asp-Glu-Val-Asp-aminometh

74 diated proteolysis of the membrane-permeable fluorescent substrate N-succinyl-L-leucyl-L-leucyl-L-val

75 the design strategy, a potential two-photon fluorescent substrate (NN) for CYP3A4 was effectively se

76 of the mutant proteins was able to transport fluorescent substrates nor had detectable basal nor drug

77 Using a near-infrared fluorescent substrate of beta-galactosidase (9H-{1,3-dic

78 ted by enzymatic cleavage of the CCF2 dye, a fluorescent substrate of beta-lactamase (BlaM), loaded i

79 dies to the surface antigen Ariel and with a fluorescent substrate of cysteine proteinases.

80 ime monitoring of transport function using a fluorescent substrate of DAT, 4-(4-(dimethylamino)styryl

81 loyl] derivative of ATP (mant-ATP) is a good fluorescent substrate of Rho and is hydrolyzed with a K(

82 Capillary luminal accumulation of fluorescent substrates of P-glycoprotein and Bcrp was de

83 ecessary for blocking transport of the known fluorescent substrate rhodamine 123.

84 By flow cytometric analysis using the fluorescent substrates rhodamine 123 and mitoxantrone, w

85 d that Q2 inhibited the efflux of a range of fluorescent substrates (rhodamine 123, doxorubicin, mito

86 y MRK-16) and P-gp function (measured with a fluorescent substrate, rhodamine 123) was characterized

87 Previously we introduced a fluorescent substrate similar to MPP+, which allowed sep

88 Y1044W, or Y401F/Y1004F mutants transported fluorescent substrates similar to the wild-type protein.

89 A novel fluorescent substrate (termed FRET-HA) to quantitatively

90 or allowed us to develop a new assay using a fluorescent substrate to measure the phospholipase activ

91 e we use a mutational approach and synthetic fluorescent substrates to define the boundaries of the h

92 imultaneously with multiple counterselection fluorescent substrates to isolate rare enzyme variants t

93 Analysis of multiple fluorescent substrates together with molecular dynamics

94 An optimized assay based on a fluorescent substrate was applied to measure the ASM act

95 A novel fluorescent substrate was devised for the sirtuin (SIRT)

96 MMP activity measured using a quenched fluorescent substrate was negligible during the first 2

97 Moreover, the fluorescent substrate was shown to be readily taken up b

98 oscopy and measuring luminal accumulation of fluorescent substrates, we assessed the transport activi

99 When these fluorescent substrates were applied to human liver micro

100 Fluorescent substrates with these reporters displayed a

101 we show that guanidinium-rich siCPDs grow on fluorescent substrates within minutes under the mildest