ライフサイエンスコーパス: formyltetrahydrofolate

1 folates as N5-methyltetrahydrofolate and N5-formyltetrahydrofolate.

2 pemetrexed activity in growth medium with 5-formyltetrahydrofolate.

3 a complex with uridine monophosphate and N-5-formyltetrahydrofolate.

4 conversion of methenyltetrahydrofolate to 5-formyltetrahydrofolate.

5 ersion of 5,10-methenyltetrahydrofolate to 5-formyltetrahydrofolate.

6 as catalytically inactive and did not bind 5-formyltetrahydrofolate.

7 lysis of 5, 10-methenyltetrahydrofolate to 5-formyltetrahydrofolate.

8 s the reversible ATP-driven production of 10-formyltetrahydrofolate (10-formyl-H(4)PteGlu(n)) from fo

9 (MTF) located in mitochondria and uses N(10)-formyltetrahydrofolate (10-formyl-THF) as the formyl don

10 hydrofolate dehydrogenase (FDH), converts 10-formyltetrahydrofolate (10-formyl-THF) to tetrahydrofola

11 explained by the rapid oxidation of (6S)-10-formyltetrahydrofolate (10-HCO-THF), which is produced b

12 ed in over a 6-fold increase in [(13)C(5)]-5-formyltetrahydrofolate ([(13)C(5)]-5-formylTHF) concentr

13 ligase (5-FCL) catalyzes the conversion of 5-formyltetrahydrofolate (5-CHO-H(4)PteGlu(n)) to 5,10-met

14 hermodynamic parameters for the binding of 5-formyltetrahydrofolate (5-CHO-H4PteGlun) and its polyglu

15 With 5-formyltetrahydrofolate (5-CHO-THF) as the folate source

16 tant L1210 clonal variant in which MTX and 5-formyltetrahydrofolate (5-CHO-THF) influx was markedly d

17 5-Formyltetrahydrofolate (5-CHO-THF) is formed by a side r

18 5-Formyltetrahydrofolate (5-CHO-THF) is formed via a secon

19 5-Formyltetrahydrofolate (5-formylTHF) is the only folate

20 of the tight-binding dimer are occupied by 5-formyltetrahydrofolate (5-formylTHF), whose N5-formyl ca

21 Transport of 5-formyltetrahydrofolate (5-FTHF) into primary cultured ce

22 inistered unnatural carbon-6 isomers, (6R)-5-formyltetrahydrofolate (5-HCO-THF) and (6S)-5,10-metheny

23 respiration of mitochondria, whereas (6S)-5-formyltetrahydrofolate (5-HCO-THF) was inactive.

24 mide ribonucleotide transformylase (GART; 10-formyltetrahydrofolate:5'-phosphoribosylglycinamide form

25 ured by 5-methyltetrahydrofolate (5MeTHF), 5-formyltetrahydrofolate (5FoTHF), and folic acid concentr

26 ate-mediated one-carbon metabolism (FOCM), 5-formyltetrahydrofolate (5fTHF), a one-carbon substituted

27 ahydrofolate dehydrogenase (FDH) converts 10-formyltetrahydrofolate, a precursor for nucleotide biosy

28 dihydrofolate reductase activity, lowered 10-formyltetrahydrofolate abundance, downregulation of DHFR

29 Binding of an N-10-formyltetrahydrofolate analogue was modeled into the str

30 rms of folates - 5-methyltetrahydrofolate, 5-formyltetrahydrofolate and 10-formyltetrahydrofolate - t

31 e domain that removes a formyl group from 10-formyltetrahydrofolate and a NADP(+)-dependent dehydroge

32 FS) is the only enzyme known to metabolize 5-formyltetrahydrofolate and catalyzes the conversion of 5

33 approximately 50% decrease in the EC50 for 5-formyltetrahydrofolate and folic acid and the MTX IC50 r

34 The GART activity of GART requires 10-formyltetrahydrofolate and has been a target for anti-ca

35 for (6S)-N5-methyltetrahydrofolate, (6S)-N5-formyltetrahydrofolate and methotrexate compared to the

36 ic production of 5-methyltetrahydrofolate, 5-formyltetrahydrofolate and tetrahydrofolate after 48 h o

37 ormylfolic acid, 5-methyltetrahydrofolate, 5-formyltetrahydrofolate and tetrahydrofolate were determi

38 xymethyltransferase-catalyzed formation of 5-formyltetrahydrofolate, and 5, 10-hydroxymethylenetetrah

39 greater proportions of pteroylmonoglutamate, formyltetrahydrofolate, and 5,10-methenyltetrahyrofolate

40 resulted in augmentation of methotrexate, 5-formyltetrahydrofolate, and 5-methyltetrahydrofolate ini

41 E45K all 1) increased carrier affinity for 5-formyltetrahydrofolate approximately 4-fold, 2) increase

42 The Km values for AICAR and (6R,6S)10-formyltetrahydrofolate are 16.8 microM +/- 1.5 and 60.2

43 ormyltransferase, which, like MTF, use N(10)-formyltetrahydrofolate as a formyl group donor.

44 ther antifolates in HepG2 cells grown with 5-formyltetrahydrofolate at physiological pH.

45 nal Rossmann fold domain containing the N-10-formyltetrahydrofolate binding site and a C-terminal sub

46 (GAR binding region) and the C-terminal (N10-formyltetrahydrofolate binding) region of PurU.

47 ling studies, is known to be the site of N10-formyltetrahydrofolate binding.

48 hile the formyl group is transferred from 10-formyltetrahydrofolate by direct nucleophilic attack by

49 ndent inhibition by the substrate analog, N5-formyltetrahydrofolate (CHO-H(4)folate).

50 with MTHFD1, an enzyme which supplies the 10-formyltetrahydrofolate cofactor essential for DNPB.

51 rahydrofolate synthase (MTHFS; also called 5-formyltetrahydrofolate cyclo-ligase; EC 6.3.3.2) activit

52 5-Formyltetrahydrofolate cycloligase (5-FCL) catalyzes the

53 We have identified the 5-formyltetrahydrofolate cycloligase gene (FTL_0724) as be

54 10-Formyltetrahydrofolate dehydrogenase (10-FTHFDH) is a fo

55 The liver cytosolic enzyme, 10-formyltetrahydrofolate dehydrogenase (FDH) (EC 1.5.1.6)

56 10-Formyltetrahydrofolate dehydrogenase (FDH) catalyzes an

57 The enzyme 10-formyltetrahydrofolate dehydrogenase (FDH) catalyzes con

58 10-Formyltetrahydrofolate dehydrogenase (FDH) catalyzes the

59 10-Formyltetrahydrofolate dehydrogenase (FDH) consists of t

60 10-Formyltetrahydrofolate dehydrogenase (FDH) converts 10-f

61 10-Formyltetrahydrofolate dehydrogenase (FDH) is composed o

62 s identified as an additional activity of 10-formyltetrahydrofolate dehydrogenase (FDH) protein.

63 The enzyme, 10-formyltetrahydrofolate dehydrogenase (FDH), converts 10-

64 one of the enzymes of folate metabolism, 10-formyltetrahydrofolate dehydrogenase (FDH), requires a 4

65 the multidomain, multifunctional enzyme, 10-formyltetrahydrofolate dehydrogenase (FDH), using a bacu

66 The C-terminal domain (C(t)-FDH) of 10-formyltetrahydrofolate dehydrogenase (FDH, ALDH1L1) is a

67 Cytosolic 10-formyltetrahydrofolate dehydrogenase (FDH, ALDH1L1) is a

68 We conclude that the 10-formyltetrahydrofolate dehydrogenase activity of FDH is

69 th alanines resulted in total loss of the 10-formyltetrahydrofolate dehydrogenase activity, whereas t

70 viously purified COOH-terminal domain had 10-formyltetrahydrofolate dehydrogenase activity.

71 ctivity, and the full-length FDH produces 10-formyltetrahydrofolate dehydrogenase activity.

72 zyme catalytic centers but is crucial for 10-formyltetrahydrofolate dehydrogenase activity.

73 rity between the amino-terminal domain of 10-formyltetrahydrofolate dehydrogenase and methionyl-tRNA-

74 Passage of a reaction mixture of 10-formyltetrahydrofolate dehydrogenase down a size exclusi

75 e model have shown a crucial role for the 10-formyltetrahydrofolate dehydrogenase enzyme in serine-to

76 10-Formyltetrahydrofolate dehydrogenase is also known to ex

77 Coupling the 10-formyltetrahydrofolate dehydrogenase reaction to an exce

78 A new rapid procedure for purifying 10-formyltetrahydrofolate dehydrogenase results in 90 mg of

79 lysis of the product inhibition curve for 10-formyltetrahydrofolate dehydrogenase shows that H4-PteGl

80 There is a several-fold excess of 10-formyltetrahydrofolate dehydrogenase subunits in liver r

81 FDH (10-formyltetrahydrofolate dehydrogenase) is strongly downre

82 ALDH1L1 (10-formyltetrahydrofolate dehydrogenase), an enzyme of fola

83 ependent crystallographic support in work on formyltetrahydrofolate dehydrogenase, work which further

84 how much of a physiologic dose of [(13)C5]5-formyltetrahydrofolate delivered in a pH-sensitive enter

85 residue N-terminal domain catalyzes the N-10-formyltetrahydrofolate-dependent formylation of the 4''-

86 id to UDP-4-keto-arabinose and (ii) the N-10-formyltetrahydrofolate-dependent formylation of UDP-4-am

87 -pentapyranosyl-4' '-ulose] and (2) the N-10-formyltetrahydrofolate-dependent formylation of UDP-Ara4

88 f [(3)H]5-methyltetrahydrofolate and [(3)H]5-formyltetrahydrofolate (di- through heptaglutamates).

89 transport, subsequent pemetrexed and (6S)-5-formyltetrahydrofolate export into the cytosol was marke

90 The folate derivative 5-formyltetrahydrofolate (folinic acid; 5-CHO-THF) was dis

91 in bears a folate binding site, possesses 10-formyltetrahydrofolate hydrolase activity, and exists as

92 ino-terminal domain (residue 1-310) bears 10-formyltetrahydrofolate hydrolase activity, the carboxyl-

93 he Escherichia coli genes purU and purN, N10-formyltetrahydrofolate hydrolase and glycinamide ribonuc

94 N10-formyltetrahydrofolate hydrolase uses water to cleave N1

95 enyltetrahydrofolate is hydrolyzed to only 5-formyltetrahydrofolate if reducing agents are present or

96 yl group of serine and the formyl group of 5-formyltetrahydrofolate in complexes of these species wit

97 drofolate, 5'-methyltetrahydrofolate, and 5'-formyltetrahydrofolate in human plasma.

98 In order to address the function of 5-formyltetrahydrofolate in mammalian cells, intracellular

99 a likely source of CO(2) production from 10-formyltetrahydrofolate in mitochondria and plays an esse

100 anisms, the addition of p-aminobenzoate or 5-formyltetrahydrofolate in the external medium restored t

101 olysis of 5,10-methenyltetrahydrofolate to 5-formyltetrahydrofolate in vivo, and there is no requirem

102 ce large quantities of folate, and [(13)C5]5-formyltetrahydrofolate infused during colonoscopy is abs

103 These results suggest that 5-formyltetrahydrofolate inhibits serine hydroxymethyltran

104 drofolate hydrolase uses water to cleave N10-formyltetrahydrofolate into tetrahydrofolate and formate

105 ha-(32)P]UDP-l-Ara4N is formylated when N-10-formyltetrahydrofolate is included.

106 The metabolic role of 5-formyltetrahydrofolate is not known; however, it is an i

107 Finally, 10-formyltetrahydrofolate is susceptible to iron-catalyzed

108 hereas folE thyA mutants supplemented with 5-formyltetrahydrofolate (lacking pterins and depleted in

109 rofolate in mammalian cells, intracellular 5-formyltetrahydrofolate levels were depleted in human 5Y

110 indicated that HypX releases CO using N(10)-formyltetrahydrofolate (N(10)-formyl-THF) as the substra

111 nt in mouse liver and kidney does not bind 5-formyltetrahydrofolate, nor does it oligomerize with the

112 mined the effects of depleting cytoplasmic 5-formyltetrahydrofolate on cellular folate concentrations

113 e N terminus of slr0642) enabled growth on 5-formyltetrahydrofolate or folic acid but not on 5-formyl

114 in the presence and absence of glycine and 5-formyltetrahydrofolate pentaglutamate.

115 at results from the binding of glycine and 5-formyltetrahydrofolate polyglutamate, a slow tight-bindi

116 replaced with the more physiological 25 nM 5-formyltetrahydrofolate, R5 cells were 2-fold collaterall

117 nd was purified from the culture medium by 5-formyltetrahydrofolate-Sepharose affinity chromatography

118 Formyltetrahydrofolate synthetase (FTHFS) from the therm

119 wed that the abundance of genes encoding the formyltetrahydrofolate synthetase (fthfs, homoacetogens)

120 nase/methenyltetrahydrofolate cyclohydrolase/formyltetrahydrofolate synthetase (MTHFD1) for an associ

121 nase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase (MTHFD1) R653Q, may mo

122 nase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase (MTHFD1) rs2236225, in

123 drofolate dehydrogenase, cyclohydrolase, and formyltetrahydrofolate synthetase 1 (MTHFD1), we discove

124 ydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1).

125 n of the methyl groups from choline requires formyltetrahydrofolate synthetase encoded by fhs in R. p

126 The subunit of N(10)-formyltetrahydrofolate synthetase is composed of three d

127 antitative PCR of the functional marker gene formyltetrahydrofolate synthetase showed that its expres

128 hylenetetrahydrofolate dehydrogenase, and 10-formyltetrahydrofolate synthetase were also evaluated.

129 luciferase mRNA binds to the cSHMT.glycine.5-formyltetrahydrofolate ternary complex with an apparent

130 hydrofolate, 5-formyltetrahydrofolate and 10-formyltetrahydrofolate - their stabilities during microw

131 ydrofolate and catalyzes the conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate.

132 Uniquely, they preferred 10-formyltetrahydrofolate to any physiological tetrahydropt

133 lyzes the NADP(+)-dependent conversion of 10-formyltetrahydrofolate to CO(2) and tetrahydrofolate (TH

134 se catalyses the transfer of formyl from N10-formyltetrahydrofolate to GAR to yield formyl-GAR and te

135 that catalyzes the oxidative catabolism of 5-formyltetrahydrofolate to p-aminobenzoylglutamate and a

136 sm highly expressed in liver, metabolizes 10-formyltetrahydrofolate to produce tetrahydrofolate (THF)

137 It converts 10-formyltetrahydrofolate to tetrahydrofolate and CO(2) in

138 ion, i.e. NADP(+)-dependent conversion of 10-formyltetrahydrofolate to tetrahydrofolate and CO(2), co

139 actions: the NADP+-dependent oxidation of 10-formyltetrahydrofolate to tetrahydrofolate and CO2 and t

140 mains of FDH and allows the conversion of 10-formyltetrahydrofolate to tetrahydrofolate and CO2.

141 enase reaction resulting in conversion of 10-formyltetrahydrofolate to tetrahydrofolate and CO2.

142 2 and the NADP+-independent hydrolysis of 10-formyltetrahydrofolate to tetrahydrofolate and formate.

143 hydrogenase (FDH) catalyzes conversion of 10-formyltetrahydrofolate to tetrahydrofolate in either a d

144 talyzes transfer of a formyl group from N-10-formyltetrahydrofolate to the 4'-amine of UDP-L-Ara4N.

145 ltetrahydrofolate or folic acid but not on 5-formyltetrahydrofolate triglutamate, demonstrating that

146 e site of ArnA transformylase and other N-10-formyltetrahydrofolate-utilizing enzymes suggests that t

147 hares some sequence identity with several 10-formyltetrahydrofolate-utilizing enzymes.

148 olic acid was a much better substrate, and 5-formyltetrahydrofolate was a poorer substrate for transp

149 acid, (6S)5-methyltetrahydrofolate, or (6S)5-formyltetrahydrofolate was not detected.

150 drofolate, 5'-methyltetrahydrofolate, and 5'-formyltetrahydrofolate were 1250, 400, and 360 pmol/L of

151 degradation in vitro with the exception of 5-formyltetrahydrofolate, which may be a storage form of f

152 folinic acid (also known as leucovorin or 5-formyltetrahydrofolate), whose metabolic function remain