ライフサイエンスコーパス: furrow

1 ortical domains are bisected by the cleavage furrow.

2 E)-based vesicle delivery to the cytokinesis furrow.

3 at occurs several hours after passage of the furrow.

4 efects in contractile forces at the cleavage furrow.

5 express both NMIIA and NMIIB at the cleavage furrow.

6 (FAs) controls the symmetry of the cleavage furrow.

7 il resolution of DNA trapped in the cleavage furrow.

8 paired accumulation of actin in the cleavage furrow.

9 ly active RhoA induced NMIIA at the cleavage furrow.

10 ology is the ingression of a plasma membrane furrow.

11 alization of NMIIA but not of NMIIB from the furrow.

12 uring invagination of the Drosophila ventral furrow.

13 astral microtubules to position the cleavage furrow.

14 th determine the position of the cytokinetic furrow.

15 dle apparatus and ultimately the cytokinetic furrow.

16 spholipids to anchor anillin at the cleavage furrow.

17 le bundles out to the cortex to position the furrow.

18 rict the contractile ring to form a cleavage furrow.

19 erses direction toward the emerging cleavage furrow.

20 ives polar bodies and the ICSI site into the furrow.

21 brate neural tube and the Drosophila ventral furrow.

22 centralspindlin that instructs the cleavage furrow.

23 n and restricted to a narrow zone within the furrow.

24 tension are directed along the length of the furrow.

25 neuronal specification at the morphogenetic furrow.

26 an mobilise grains to form (i) pits and (ii) furrows.

27 and its loss resulted in short, disorganized furrows.

28 upts the hexagonal packing of the ingressing furrows.

29 tant even though it could form eisosomes and furrows.

30 nd distribution of Diaphanous and F-actin at furrows.

31 low and define the hexagonal symmetry of the furrows.

32 es for the Pkh protein kinases in regulating furrows.

33 d Buc-GFP, accumulates at embryonic cleavage furrows.

34 dorsal groove, and presence of deep pits and furrows.

35 additional mechanisms can recruit Steppke to furrows.

36 region was required for Sstn localization to furrows.

37 fracture analogue to explain the creation of furrows.

38 In frogs, only sister aster pairs induce furrows.

39 This explains why only sister pairs induce furrows.

40 ilaments that curve the membrane to form the furrows.

41 s the pattern of Rho activation in incipient furrows.

42 ntous actin to actin caps and pseudocleavage furrows.

43 regression rather than failed initiation of furrowing.

44 ty is responsible for asymmetric cytokinetic furrowing.

45 on of ingressing membrane during cytokinetic furrowing.

46 any cell shape changes including cytokinetic furrowing.

47 g it unlikely that an actomyosin ring drives furrowing.

48 oss-linkers affects the speed of cytokinetic furrowing.

49 th other factors, organize into the cleavage furrow [1].

50 y spermatocytes that fail to form a cleavage furrow [3] and during monopolar cytokinesis when myosin

51 model requires the presence of the cephalic furrow, a fold located anteriorly of the extending tissu

52 that RMD1 is required for myosin II cleavage furrow accumulation, acting in parallel with mechanical

53 identify factors involved in myosin cleavage furrow accumulation.

54 uirement for membrane trafficking to support furrow advancement.

55 activated if DNA is retained in the cleavage furrow after completion of anaphase.

56 hase and anaphase), spindle midzone/cleavage furrow (anaphase and telophase), and midbody (cytokinesi

57 cron-scale contours, such as the cytokinetic furrow and base of neuronal branches.

58 tructure that anchors the ingressed cleavage furrow and guides the assembly of abscission machinery.

59 odeling is thought to stabilize the cleavage furrow and maintain cell shape during cytokinesis [1-14]

60 ositioned to coordinate trafficking into the furrow and mark the center of the cell in lieu of a midb

61 omain protein that localizes to the cleavage furrow and midbody of mitotic cells, where it is require

62 The distribution of pPLK4 midzone/cleavage furrow and midbody positions pPLK4 to play a functional

63 In the Drosophila ventral furrow and other epithelia, apical constriction of hundr

64 ranslocation to the spindle midzone/cleavage furrow and prevents cellular abscission, leading to the

65 TP in epithelial cells--RhoA at the cleavage furrow and RhoA and Rac1 at cell-cell junctions.

66 lized myosin is thus critical to ensure that furrow and spindle midzone positions coincide throughout

67 s essential to inhibit myosin and coordinate furrow and spindle positions during asymmetric division.

68 n-scale curvature, including the cytokinetic furrow and the base of cell protrusions.

69 tile ring, actin was not concentrated in the furrow and was not directly required for furrow progress

70 Here, we investigate whether polarized furrowing and abscission in mouse NSCs are regulated dif

71 osin contractile ring that drives equatorial furrowing and separation into the two daughter cells.

72 severe rings, strictures, or combination of furrows and edema.

73 kif5Ba is required to enrich Buc at cleavage furrows and for the ability of Buc to promote excess PGC

74 Iris furrows and iris color were not associated with iris vol

75 efects, although pkh2 cells formed chains of furrows and pkh3 cells formed wider furrows, identifying

76 kif5Ba is required to recruit GP to cleavage furrows and thereby specifies PGCs.

77 MIIB]), only NMIIB localizes at the cleavage furrow, and its subsequent absence contributes to polypl

78 the ring, expand the plasma membrane in the furrow, and separate the daughter cell membranes.

79 ted to the adherens junction at the cleavage furrow, and that inhibiting recruitment of Vinculin by e

80 ssociation of microtubules with the cleavage furrow, and we used a fluorescently tagged EB1 protein t

81 tous fungi and dendritic spines, in cleavage furrows, and in retracting membrane protrusions in mamma

82 Synd localized to these furrows, and its loss resulted in short, disorganized fu

83 RalA localizes to syncytial furrows, and mediates the movement of exocytic vesicles

84 surface irrigation systems, long border and furrow are more adaptive to mechanized farming but may c

85 nd whether it acts through Galpha to promote furrowing are unclear.

86 Trilobate morphology and effaced transverse furrows are locally evident, but the predominant morphol

87 racterizing the mechanisms that position the furrow, assemble the contractile ring, anchor the ring t

88 mal-vegetal axis after ICSI, with asymmetric furrows assembling from the male pronucleus.

89 e plane displacement with mislocalization of furrow-associated markers, associated with actomyosin dy

90 sting that TOE-2 might position the cleavage furrow asymmetrically to generate daughter cells of diff

91 e isoforms, only NMIIB could localize at the furrow at low RhoA activity.

92 RhoA activation is sufficient to generate furrows at both the cell equator and cell poles, in both

93 Eisosomes are membrane furrows at the cell surface of yeast that have been show

94 r Arf1 to function at the Golgi for cleavage furrow biosynthesis.

95 nomically diverse eukaryotic cells divide by furrowing but have no type-II myosin, making it unlikely

96 provide the ingression force for cytokinetic furrows, but the role of membrane trafficking pathways i

97 the abundant NMIIA does not localize at the furrow by focusing on the RhoA/ROCK pathway that has a l

98 tion proteins are stabilized at the cleavage furrow by increased tension.

99 d graded for crypts (by number and size) and furrows (by number and circumferential extent) following

100 and water at the cell poles and the division furrow can also achieve the same type of shape change du

101 ssembly, fails to accumulate at the cleavage furrow, cannot rescue myoII-null cytokinesis, and has im

102 e base of pseudocleavage and cellularization furrows, closely mimicking Steppke loss-of-function embr

103 vature-mediated filament alignment speeds up furrow closure while promoting energy efficiency.

104 Here, we report that, after cleavage furrow closure, septins form a membrane-bound double rin

105 uatorial proteins was greatly reduced at the furrow compared to the interphase cortex, suggesting the

106 role of turnover on the rate and success of furrow constriction.

107 specifies the cleavage plane, and regulates furrow contractility.

108 r ring constriction, produce force to form a furrow, disassemble the ring, expand the plasma membrane

109 potential channel, localizes to the cleavage furrow during cytokinesis of the fission yeast, Schizosa

110 additionally, it accumulates in the cleavage furrow during cytokinesis.

111 required for internalization of the ventral furrow during gastrulation.

112 The geometry of the cleavage furrow during mitosis is often asymmetric in vivo and pl

113 nt cells initiate the ingression of cleavage furrows earlier than normal, shortening the stage of cyt

114 nd plays an important role in pseudocleavage furrow extension, and this role is also likely to be cru

115 erexpression in rhogef2 mutants reversed its furrow-extension phenotypes, Peanut and Diaphanous recru

116 the cytokinetic ring, and faster cytokinetic furrowing, following depletion of GCK-1 or CCM-3.

117 Furrows form within 30 degrees of the animal pole, but t

118 ial for cytokinesis in fission yeast, proper furrow formation also requires septum deposition.

119 ion, suggesting a relationship with cleavage furrow formation and abortive cytokinesis.

120 RalA function is absolutely required for furrow formation and initiation.

121 ty is not sufficient to recapitulate ventral furrow formation and reveal that additional, ventral-spe

122 both microtubules and proteins necessary for furrow formation and the completion of cytokinesis.

123 nted orthogonal to the folding axis promotes furrow formation at lower contractility levels.

124 Remarkably, furrow formation can be initiated in rounded interphase

125 tion during the lengthening phase of ventral furrow formation can be precisely described by viscous f

126 RhoA controls cleavage furrow formation during cell division, but whether RhoA

127 ole is also likely to be crucial in cleavage furrow formation during cell division.

128 It is widely believed that cleavage-furrow formation during cytokinesis is driven by the con

129 Cortical F-actin prior to furrow formation fails to exhibit a normal transition in

130 d Goltzer show that RhoA activity can induce furrow formation in all cell cortex positions and cell c

131 and the exocyst complex, that mediates rapid furrow formation in early Drosophila embryos.

132 Furrow formation in early syncytial Drosophila embryos i

133 pindle pole body help coordinate cytokinetic furrow formation in fission yeast.

134 Furthermore, furrow formation in response to RhoA activation is not t

135 When Rab8 function is disrupted, furrow formation in the early embryo is completely aboli

136 athway that is essential for plasma membrane furrow formation in vivo.

137 yosin ring as a mechanical structure driving furrow formation is better understood, its role as a spa

138 Plasma membrane furrow formation is crucial in cell division and cytokin

139 of membrane trafficking pathways in guiding furrow formation is less clear.

140 es to orchestrate spatiotemporal dynamics of furrow formation is unknown.

141 ompression by flow or active alignment drive furrow formation remains unclear.

142 improve the efficiency of a core process for furrow formation that was already present in ancestral e

143 est that Dop impinges upon the initiation of furrow formation through developmental regulation of cyt

144 level, extension is coincident with ventral furrow formation, and at a cellular level, extension occ

145 This is independent of furrow formation, centrosomes and microtubules and, inst

146 that during the lengthening phase of ventral furrow formation, hydrodynamic behaviour of the cytoplas

147 Here, we use 4D imaging to identify furrow formation, stabilization, and regression periods,

148 Ventral furrow formation, the first step in Drosophila gastrulat

149 uch rings, they are not always essential for furrow formation.

150 the cytoskeleton and tension during ventral furrow formation.

151 en Nuf and Polo with respect to Nuf-mediated furrow formation.

152 lity that the microtubules are important for furrow formation.

153 e for Synd in actin-regulated pseudocleavage furrow formation.

154 s essential for central spindle and cleavage furrow formation.

155 ile rings and that cell rounding facilitates furrow formation.

156 cytoplasm and plasma membrane during ventral furrow formation.

157 e-level behaviors that emerge during ventral furrow formation.

158 sophila embryos is exceptionally rapid, with furrows forming in as little as 3.75 min.

159 In the absence of RalA and furrow function, chromosomal segregation is aberrant and

160 that midzone MTs become highly stable after furrows have begun ingression [2], indicating that furro

161 hains of furrows and pkh3 cells formed wider furrows, identifying novel roles for the Pkh protein kin

162 es force to ingress the cytokinetic cleavage furrow in animal cells, yet its filament organization an

163 tacts also direct ingression of the cleavage furrow in coordination with FAs in epithelial cells-MDCK

164 How myosin II localizes to the cleavage furrow in Dictyostelium and metazoan cells remains large

165 ological problem-positioning of the cleavage furrow in dividing cells-to explain how and why DE and A

166 LET-99 localizes to the presumptive cleavage furrow in response to the spindle and myosin.

167 ial cell death anterior to the morphogenetic furrow in the eye disc and within wing pouch in the wing

168 The cleavage furrow in Xenopus zygotes is positioned by two large mic

169 Here we show that LET-99 contributes to furrowing in both asymmetrically and symmetrically divid

170 T-99 enhances myosin accumulation to promote furrowing in both symmetrically and asymmetrically divid

171 INCENP overexpression rescues furrowing in MKlp2-depleted cells in an INCENP-actin bin

172 t microtubules are still associated with the furrows in the absence of F-actin, consistent with the p

173 hich correspond to approximately 200-nm-long furrows in the plasma membrane.

174 complex subunit and localizes to ingressing furrows in wild-type embryos, becomes punctate and loses

175 narrow epithelial furrow, termed the ventral furrow, in which actomyosin fibres and tension are direc

176 t only sister asters recruited two conserved furrow-inducing signaling complexes, chromosome passenge

177 clude that if multiple signals contribute to furrow induction in echinoderm embryos, they likely conv

178 As furrows ingress, cytoplasmic Rab8 puncta are depleted an

179 e., substrate attached side) of the cleavage furrow ingressed less than the top (i.e., unattached sid

180 The first furrow ingresses through the egg midplane, which is dema

181 KIF20B depletion affects the speed of both furrow ingression and abscission.

182 nhibition of these calcium spikes slowed the furrow ingression and led to frequent lysis of daughter

183 ng that there is active coordination between furrow ingression and microtubule dynamics.

184 We developed methods to live image furrow ingression and midbody abscission in NSCs within

185 growth is rapid but regulated for cycles of furrow ingression and regression.

186 calcium spikes both at the start of cleavage furrow ingression and the end of cell separation.

187 Of interest, ring closure and hence the furrow ingression are nonconcentric (asymmetric) within

188 During early divisions, furrow ingression asymmetry and midbody inheritance is c

189 odel in which active Rab8 populations direct furrow ingression by guiding the targeted delivery of cy

190 promotes microvillar F-actin assembly, while furrow ingression controls microvillar F-actin disassemb

191 otein dynamics has allowed us to account for furrow ingression during cytokinesis, a model cell-shape

192 RhoA promotes contractile ring assembly and furrow ingression during cytokinesis.

193 ila embryo undergoes several cycles of rapid furrow ingression during early development that culminat

194 t link compartmental behaviors with cortical furrow ingression events are unclear.

195 served Arf GAP Asap is required for cleavage furrow ingression in the early embryo.

196 s-like protein A (RalA) is required for fast furrow ingression in the early fly embryo.

197 arization microscopy and found that cleavage furrow ingression initiates by contraction of an equator

198 on yeast Schizosaccharomyces pombe, cleavage furrow ingression is driven by polymerization of cell wa

199 We find that polarized furrow ingression occurs at a steady rate and completes

200 e overall reservoir is depleted in sync with furrow ingression over 60-70 min.

201 sensitize mto1Delta and mto2(S338N) cells to furrow ingression phenotypes.

202 le ring, and acute inhibition of CYK-1 after furrow ingression slows ring constriction rate, suggesti

203 ggests that the CPC is integral for coupling furrow ingression with midzone microtubule stabilization

204 art after mitosis, preparing a soft path for furrow ingression, and releasing G-actin from internal n

205 e sequential steps of cytokinesis, including furrow ingression, membrane resolution and cell separati

206 y at the division plane just before cleavage furrow ingression, suggesting that these calcium transie

207 ply from exocytosis and membrane demand from furrow ingression.

208 rvoir of microvilli unfolds to fuel cleavage furrow ingression.

209 s and for midzone MT stabilization following furrow ingression.

210 ted substrate attachment could be regulating furrow ingression.

211 minant-negative mutant increases the rate of furrow ingression.

212 e of two pathways that can initiate cleavage furrow ingression.

213 orks to build cortical networks that support furrow ingression.

214 hways converge to ensure successful cleavage furrow ingression.

215 e formation and defects in cytokinesis after furrow ingression.

216 nt for intracellular trafficking pathways in furrow ingression; however, the pathways that link compa

217 CYK-4, a centralspindlin component, promotes furrow initiation in C. elegans embryos and human cells.

218 ugh depletion of nonmuscle myosin II delayed furrow initiation, slowed F-actin alignment, and reduced

219 Synd and Peanut were both absent in furrow-initiation mutants of RhoGEF2 and Diaphanous and

220 By manipulating the cleavage furrow into different shapes, we show that the ring prom

221 Furrow irrigation may store slightly more water in the t

222 56.63, and significantly lower than that for furrow irrigation over the whole growth duration.

223 Border and furrow irrigation were tested using fertilizer rates of

224 der irrigation was 0.8% larger than that for furrow irrigation.

225 The inactivation of Rac1 at the cleavage furrow is controlled by MgcRacGAP.

226 During animal cell division, the cleavage furrow is positioned by microtubules that signal to the

227 ng constriction, but the rim of the cleavage furrow is the main site for endocytosis.

228 erogeneity along the length of the border or furrow is weak for soil water content and is moderate fo

229 nd nitrate along the length of the border or furrow is weak or medium, which can create non-uniform c

230 alian cells and demonstrate that cytokinetic furrowing is primarily regulated at the level of RhoA ac

231 Elevating Sstn furrow levels had no effect on the steppke phenotype, bu

232 the steppke phenotype, but elevating Steppke furrow levels reversed the sstn phenotype, suggesting th

233 ularity, allowing it to propagate, leaving a furrow-like scar in its wake.

234 erization, and its removal disrupted Steppke furrow localization and activity in vivo.

235 ane, we observed the recruitment of cleavage furrow markers, including an active RhoA reporter, at mi

236 aberrant cytoskeletal reorganization during furrow maturation, including abnormal F-actin enrichment

237 division cycles in Drosophila Pseudocleavage furrow membranes in the syncytial Drosophila blastoderm

238 al differentiation by blocking morphogenetic furrow (MF) progression and R8 specification.

239 x, lamellar actin networks, and the cleavage furrow of dividing cells--always together with myosin-II

240 ading edge of motile cells, and the cleavage furrow of dividing cells.

241 sma membrane and accumulated in the cleavage furrow of the Q.a and Q.p neuroblasts, suggesting that T

242 Even with longer borders or furrows of 90 m, the uniformity of water and nitrate alo

243 eton activity at the base of plasma membrane furrows of the syncytial embryo.

244 biquitous in nature and technology, from the furrows on our foreheads to crinkly plant leaves, from r

245 hales (Mysticeti) in possessing longitudinal furrows or grooves in the ventral skin that extend from

246 CO2 ice and demonstrate that these resemble furrow patterns on Mars, suggesting similar formation me

247 ry are key determinants for correct cleavage furrow placement and cortical expansion, thereby establi

248 c targeting factor Rab11 is recruited to the furrow plane normally at the tip of bundling microtubule

249 g pathway is known to regulate cell division furrow position, and Hippo molecules localize to BBs and

250 zation, and Sas4 loss disrupts cell division furrow positioning and DNA segregation.

251 ortical expansion, ensuring correct cleavage furrow positioning and the establishment of physical asy

252 icrotubules (MTs) are essential for cleavage furrow positioning during cytokinesis, but the mechanism

253 polarity appears normal, and chromosome and furrow positioning remains unchanged when nmy-2 is inact

254 ymmetric cortical extension and myosin basal furrow positioning.

255 cleavage, myosin recruitment to the cleavage furrows proceeds in temporally distinct phases of tensio

256 Inhibition of membrane partitioning blocked furrow progression, indicating a requirement for membran

257 the furrow and was not directly required for furrow progression.

258 ion mutants of RhoGEF2 and Diaphanous and in furrow-progression mutants of Anillin.

259 that although F-actin is associated with the furrow region, none of the three myosins (of types VIII

260 ate, active Rap1 becomes restricted from the furrow region, where the myosin contractile ring is subs

261 Accordingly, ALIX depletion leads to furrow regression in cells with chromosome bridges, a ph

262 sis; however, cytokinesis failure stems from furrow regression rather than failed initiation of furro

263 btle Golgi reorganization preceding cleavage furrow regression.

264 ch an ectopic myosin cap shifts the cleavage furrow relative to the spindle center.

265 h new membrane are deposited to the cleavage furrow relatively evenly during contractile-ring constri

266 ough a combination of a mutation and a drug, furrows still formed and the cells divided, although som

267 e determines the position of the cytokinesis furrow, such that the contractile ring assembles in an e

268 al cells generates a long, narrow epithelial furrow, termed the ventral furrow, in which actomyosin f

269 brane in the absence of ligand, into a polar furrow that becomes accessible to lipid headgroups in th

270 , which is slightly behind the morphogenetic furrow that marks the front of PR differentiation, the m

271 ane helices constituting a membrane-spanning furrow that provides a path for lipids in scramblases ha

272 entral ridge and regularly spaced transverse furrows that convey the appearance of discrete body segm

273 n actomyosin network at the base of membrane furrows that invaginate from the surface of the embryo.

274 activity, leading to assembly of cytokinetic furrows that partially ingress.

275 N-cadherin is enriched in the post-cleavage furrow; then one cell pivots around the other, resulting

276 leads to myosin II depletion at the membrane furrow, thereby resulting in decreased cell height and l

277 le lies directly in the path of the cleavage furrow, this delay may explain, at least in part, the de

278 t impairs NMIIA localization at the cleavage furrow through increased actin turnover.

279 ing cortical interactions, leading to random furrows through the animal pole.

280 The cleavage-furrow tip adjacent to the actomyosin contractile ring i

281 lization precedes F-actin recruitment to the furrow tip, suggesting that membrane trafficking might f

282 uently be repressed behind the morphogenetic furrow to allow for neuronal differentiation.

283 tion of select target genes posterior to the furrow to ensure properly timed mitotic exit.

284 f cellular appendages and/or at the cleavage furrow to help compartmentalize the plasma membrane and

285 the uniformity along the length of border or furrow to understand the spatial and temporal distributi

286 s have begun ingression [2], indicating that furrow-to-MT communication may occur.

287 tex and induces a strong displacement of the furrow toward the anterior, which can lead to DNA segreg

288 dinated cell-shape changes to form a ventral furrow (VF) and are subsequently internalized.

289 sis, animal and fungal cells form a membrane furrow via actomyosin ring constriction.

290 show that Myosin relocalizes to the cleavage furrow via two distinct cortical Myosin flows: at anapha

291 ing division, thylakoids inside the cleavage furrow were kinked and severed.

292 n anaphase, p120 is enriched at the cleavage furrow where it binds MKLP1 to spatially control RhoA GT

293 ealed asymmetry in the shape of the cleavage furrow, where the bottom (i.e., substrate attached side)

294 have focused on the formation of the ventral furrow, whereby approximately 1,000 presumptive mesoderm

295 reorientation along the cytokinetic cleavage furrow, which might have implications for diverse other

296 ubulovesicular structures along the cleavage furrow while the exocyst tethers vesicles at the rim of

297 luding oedema, granularity, white spots, and furrowing, while histology revealed oedema, immune cell

298 d typical endoscopic features include linear furrows, white plaques, and concentric rings.

299 uncovering a feedback mechanism that couples furrowing with microtubule dynamics.

300 ntromere region, midbody, and pseudocleavage furrows without DNA damage and in addition forms numerou